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Guide for newcomers to the Biopark 2015

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Page 1: Brochure with linked files 2015-03 copy

Guide for newcomers to the Biopark

2015

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Faculté des Sciences appliquées

Guide for newcomers to the Biopark

What is the Biopark?

Advanced training on the campus

Core facilities and scientific ressources on the campus

Practical information

p. 1

p. 3

p. 9

p. 17

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What is the Biopark?

Welcome in the

This document aims at welcoming newcomers on the Gosselies campus of the ULB.

The campus is located within the Biopark Charleroi Brussels South, a biotechnology excellence cluster which involves a variety of organisations involved in scientific innovation.

These include

• two universities: the Université Libre de Bruxelles (ULB) and the Université de Mons (Umons), and their joint imaging facility (the CMMI),

• a vaccinology research center (ImmuneHealth),

• a professional training center (Biopark Formation),

• about 20 biotech companies,

• two units helping scientists to turn their research findings into business: the technology transfer office (TTO) and the i-Tech Incubator.

For more information on the Biopark Charleroi Brussels South

and the organizations that it hosts:http://www.biopark.be

Faculté des Sciences appliquées

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Wha

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ULB in the BioparkTwo faculties of the ULB are present in the Biopark: the Faculty of Sciences and the Faculty of Medicine.

The Faculty of Sciences includes, amongst others, the Département de Biologie des Organismes (DBO) and the Département de Biologie Moléculaire (DBM). Nearly all members of the DBM work on the Gosselies campus, and one lab of the DBO—the Laboratoire de Biotechnologie végétale (LBV)—is also located there.

Two institutes of the Faculty of Medicine are present in Gosselies: the Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM) and the Institute for Medical Immunology (IMI). The Faculty also comprises many labs and a university hospital on the Erasme campus in Brussels.

The DBM and the IRIBHM in Gosselies are grouped together into the Institut de Biologie et de Médecine Moléculaires (IBMM).

IRIBHM

IRIBHMGosselies

DBM IMI

IBMM

DBO

LBV BioparkFormation

CMMI

Gosselies campus

Facultés des Sciences

Faculté de Médecine

Faculté des Sciences appliquées

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Advanced training on the G

osselies campus

Advanced trainingDoctoral trainingPhD students perform two parallel tasks. First, they carry out research that they will utimately summarize into a manuscript and defend in front of a committee of experts.

Second, they follow a doctoral training to consolidate their specialized skills within their discipline and to develop transferable skills that are attractive to both the academic and private sectors. Doctoral training represents 60 ECTS and entitles the student to a Research training certificate, which must be provided when submitting the thesis.

The progression of a PhD student in his/her research and in the completion of his/her doctoral training is overseen by his/her thesis supervisor, thesis co-supervisor (if applicable), and supervisory committee. The supervisory committee comprises the thesis supervisor and at least two other academics. It must be formed before the PhD student enrols for the second year of his/herPhD, and ideally when enrolling for the first time. The supervisory committee gives its opinion on the PhD student’s re-enrolment every year. In many cases, the supervisory committee also gives its opinion about whether the thesis can be submitted.

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A specific authority (with variable names, see below) organizes

• the enrolment of PhD students

• the constitution of their supervisory committees

• the doctoral training programme and its validation by the supervisory committee

• possible intermediate evaluations (usually during the 2nd year of the PhD)

• the constitution of thesis jurys and the final evaluation of the thesis

For the DBM, this authority is the Ecole Doctorale en Biochimie, Biologie Moléculaire et Cellulaire

President: Prof. Luc Vanhamme

http://www.ulb.ac.be/facs/sciences/biol/edoct-biombiotech.html

For the DBO: the Commission doctorale en Biologie des Organismes

President: Prof. Serge Aaron

Secretary: Prof. Olivier Hardy

http://www.ulb.ac.be/facs/sciences/biol/edoct-dbv-dba.html

For the Faculty of Medicine: the Commission facultaire des doctorats

President: Prof. Philippe Lebrun

Secretary: Prof. Magali Waelbroeck

http://www.ulb.ac.be/facs/medecine/comfacdoctorats.html

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Advanced training on the G

osselies campus

The Biopark Formation Training Centre provides a broad range of theoretical and practical hands-on trainings, for those involved at all levels of the Life Sciences sector (scientists, technicians, Ph.D students, professors, and jobseekers).

These specialization courses allow trainees to improve their knowledge and skills in different fields and techniques.

Courses are divided in modules that last from 0,5 to a few days and include: general theoretical introductions, practical introduction (performed on the Biopark equipment) or more specialized modules that are often given in partnership with Biopark scientists. Each module can be followed separately.

All courses are given in the Biopark Charleroi Brussels South.

They are available at very reasonnable prices for academic workers (www.biophare.eu) and are free for the students of the Biopark campus thanks to the “Fonds de Formation”. The fund supports master students, PhD students and post-doctoral students. For more information:

Biopark Formation

www.biopark.be/bioparkformation/Fonds.html

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The following courses are organized in 2014-2015:

1) Molecular biology: PCR, q-PCR, NGS…

2) Cell biology: cell culture, GLP in cell biology techniques, stem cells…

3) Imaging fluorescence

4) Immunology: immunological techniques, cancer immunotherapies, ...

5) Flow cytometry

6) Biostatistics and Big Data mining in Life Sciences

7) Quality: a useful sets of tools for your research

The Biopark Formation catalogue increases regularly to meet the training needs of different stakeholders in the life sciences sector. The updated catalogue is available on our web site.

Biopark FormationIMI buiding (level 2)

071 37 86 [email protected]

www.biopark.be/bioparkformation

The Biopark Formation team. From left to right: Valérie Claras, Erika Baus, Christelle Debeys, Valérie Hertveldt, Béatrice Goxe, Arnaud Termonia.

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Advanced training on the G

osselies campus

English courses for PhD studentsEnglish courses are regularly organized on the campus by the graduate school in biochemistry and molecular and cellular biology. These courses focus on scientific communication in English and can therefore be recognized as part of the doctoral training.

Thanks to the financial contribution of the Fonds Brachet, these English courses are held in small groups at a reasonnable cost for the participants.

SeminarsLab meetings and journal clubs are regularly organized by each research unit, on its own or in conjunction with other teams, to discuss recent work or publications.

Monthly, 1 to 5 seminars are given by invited scientists or ULB researchers, either at the Point Centre buiding, in the IMI meeting room or in the Brachet auditorium at the IBMM.

Yearly in late May or June, students of the IBMM in the 2nd year of their PhD give an oral presentation of their work in the Brachet auditorium at the Point Centre buiding. Other PhD students are also encouraged to present a poster. These « Journées des doctorants » (PhD students’ days) end with a friendly lunch in the park of the Centre de Culture Scientifique owned by the ULB in Parentville.

For the complete list of upcoming seminars: http://www.biopark.be/biopark/news.html

You want to organize a seminar? Contact Mrs Caroline Lamy by phone (9789) or by mail at [email protected]

Contact: Prof. Luc Vanhamme

9664 - [email protected]

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Fonds Jean BrachetThe Fonds Jean Brachet is an independent, non-profit organization which was created in 1985 to honour Prof. Jean Louis Auguste Brachet as a Pioneer of Molecular Biology and a founder of the Department of Molecular Biology of the ULB. It is funded solely through donations and memberships.

The Fonds Jean Brachet (FJB) aims to support and encourage interdisciplinary research and collaboration within the Department of Molecular Biology.

The FJB contributes to research financing, technology development, equipment purchasing, but also to training and knowledge exchange. It awards grants to young researchers at the beginning of their independent careers to encourage novel approaches in research and to seed the future in science.

In particular, the FJB regularly contributes to financing the participation of young researchers to

• courses• national and international meetings• short stays in belgian laboratories

The deadlines to apply for travel grants are the 1st of February, 1st of June and 1st of October.

A short stay in a foreign laboratory may also be financed through the Paul Anciaux special grant. The annual deadline for this grant is the 1st of June.

For more information:

http://fondsbrachet.biopark-it.be

Academic contact: Dr. Sabrina Bousbata

9850– [email protected]

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Core facilities

Core facilities and scientific expertise on the campus

The proteomics facility of the DBMIBMM's proteomics section boasts a broad range of services to extract, separate and describe proteins from a large set of biological systems:

• Separation by SDS-PAGE, two-dimensional electrophoresis (2-DE), and differential gel electrophoresis (DIGE)

• 2-DE immunoblotting

• Protein digestion

• Protein/peptide sequencing by LC-MS/MS

• Protein identification through in-house Mascot

• Large-scale post-translational modifications analysis (phosphorylation, glycosylation, etc.)

• Biostatistics and data mining

Contact: Dr. Sabrina Bousbata

9850 – [email protected]

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The Center for Microscopy and Molecular Imaging (CMMI)The Center for Microscopy and Molecular Imaging (CMMI) is a preclinical imaging platform co-founded by the Université Libre de Bruxelles and the Université de Mons. CMMI research facilities have state-of-the-art equipment and offer cutting-edge technologies for imaging, ranging from electron microscopy to in vivo animal imaging. The Center provides services and training for academic laboratories as well as industrial partners.

It is organized in four sections.

1) Diapath

Diapath is an immunohistochemistry platform, oriented towards the validation of biomarkers and the standardization of immunohistochemistry processes. The services it proposes to external users range from sample preparation to data analysis:

• Cell and tissue conditioning (paraffin embedding)

• Various stainings: H&E, PAS, Masson trichrome, …

• Tissue microarray production

• Slide scanning

• Data analysis and reporting:

• qualitative and semi-quantitative scoring (double blind test)• computer-assisted quantitative analysis (area, intensity,

morphometry, …)• tissue-based biomarker colocalization

Contact: Prof. Isabelle Salmon

02 555 31 15 – [email protected]

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Core facilities

2) In vivo imaging

Magnetic resonance imaging (MRI)

=> Anatomical studies in or ex vivo, with or without contrast agents

=> Dynamic MRI (e.g. for tumor perfusion studies)

=> Functional MRI (e.g. for heart monitoring)

=> Molecular MRI, using targeted contrast agents

Optical imaging in vivo

=> Bioluminescence imaging

=> Fluorescence imaging (red and near infra-red)

Nuclear molecular imaging (nuMIx)

• PET-(CT)-scan

• SPECT-(CT)-scan

• Quantitative numerical autoradiography (ex vivo)=> Imaging of functional/metabolic processes

(e.g. uptake/excretion of substrate tracers)=> Imaging of molecular targets (e.g. proteins

specifically expressed by a tumor or graft)=> Imaging of cell trafficking and homing

3) Multimodal image processing=> Development of ad-hoc image processing solutions

(e.g. segmentation, counting, detection, etc)

=> Process automation (e.g.high troughput)

=> Image processing training

=> Custom data analysis

=> Hardware accelerationContact: Dr. Ir. Olivier Debeir

2295 – [email protected]

Contact: Prof. Serge Goldman

[email protected]

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4) Microscopy

Fluorescence microscopy

• Wide-field • Laser-scanning confocal (CLSM)• Spinning-disk confocal (SD)• High-throughput microscope

=> Localization of molecules of interest at diverse scales=> 3D reconstruction=> live imaging (including of yeast and bacteria)=> With the CLSM: spectral imaging, FRAP, FRET

Digital holographic microscopy

=> Cell counting and evaluation of cell viability for adherent cells or cells in suspension

=> Counting of lipid droplets in adipocytes

Electron microscopy

• Transmission electron microscope (TEM)=> Ultrastructural analysis => Immunolocalization

• Scanning electron microscope (SEM)=> Surface morphology analysis

Forthcoming developments (2015-2016):

Cryo-TEM and TEM-assisted tomography

Contact: Prof. Véronique Kruys

9804 – [email protected]

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Scientific expertise on the campus

Technical and scientific expertise of the laboratories on the campus

Bacterial Genetics and Physiology

PI: Laurence Van Melderen (9778 – [email protected])

Themes: •Horizontal gene transfer •Toxin/antitoxin systems •Bacterial physiology

Techniques: •Time-lapse and single cell fluorescence microscopy •Molecular microbiology •Primer extension •Northern blot •qPCR •BL2 lab

Biology of Membrane Transport

PI: Anna Maria Marini (9707 – [email protected])

Themes: •Membrane transport proteins •Rhesus factors •Cell physiology

Techniques: •Yeast and mammalian cell culture •Protein biochemistry •Gene expression analysis •Yeast and mouse phenotypic analysis

Biology of the Nucleus

PI: Birthe Fahrenkrog (9793 – [email protected])

Themes: •Nucleoporins in cell cycle regulation and cancer •Epigenetic regulation of apoptosis

Techniques: •Fluorescence microscopy •Live cell imaging •Electron microscopy •Yeast, human, mouse cells •Molecular biology

IBMM presidency: Prof. Bruno André (9958 - [email protected])

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Developmental Genetics

PI: Eric Bellefroid (9732 – [email protected])

Themes: •Neural stem cells •Neuronal specification •Cerebral cortex, •Transcriptional and epigenetic regulation

Techniques: •Mouse genetic approaches, assays in chicken and frog embryos •in situ hybridization •immunohistochemistry •ChIP •RT-qPCR

Embryology and Biotechnology

PI: Jacob Souopgui (9936 – [email protected])

Theme: •Molecular basis of epithelial-to-mesenchymal transition (EMT) in morphogenesis and diseases

Techniques: •Microinjection in developing xenopus embryos, •Transfection of specific cell lines •Profiling of biomarkers in tumor samples

Immunobiology

PIs: Muriel Moser (9863 – [email protected]), Oberdan Leo (9877 – [email protected]), Fabienne Andris (9880 – [email protected]), and Guillaume Oldenhove (9878 – [email protected])

Themes: •Antigen-presenting cells •Cancer immunotherapy •Tolerance •Helper and regulatory T cell development •Auto-immunity

Techniques: •In vivo and in vitro analyses of immune responses •Flow cytometry •In vivo inflammatory models •Fluorescence microscopy •Antibody production

Institute of Interdisciplinary Research in Human and Molecular Biology – Gosselies

PIs: Bernard Robaye (9822 – [email protected]) and Daniel Christophe (9828 – [email protected])

Themes: •Physiological roles of purinergic transmembrane receptors •Control of gene expression in the thyroid

Techniques: •Phenotypic analysis of K.O. mice •Immuno-histochemistry •Culture and differentiation of mesenchymal stem cells •Dendritic cells physiology •Gene promoter and transcription analysis (reporter gene assays, footprint, EMSA, ChIP)

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Scientific expertise on the campus

Microbiology

PIs: Louis Droogmans (9922 – [email protected]) and Sabrina Bousbata (9850 – [email protected])

Themes: •Enzymes from extremophilic organisms •tRNA modification enzymes • Host-parasite Interaction Proteomics

Techniques: •Microbial culture •Protein purification and crystallization •2DE •DIGE •LC-MS/MS •PTMs •Biostatistics

Molecular Biology of the Gene

PIs: Véronique Kruys (9804 – [email protected]) and Cyril Gueydan (9805 – [email protected])

Themes: •Post-transcriptional regulations •Nucleo-cytoplasmic transport •RNA-binding proteins •Translation

Techniques: •Mammalian and Drosophila cell culture •Northern blot •Western blot •Fluorescence microscopy •EMSA •RNAi •RT-qPCR

Molecular Cell Physiology

PI: Bruno André (9958 – [email protected])

Themes: •Intracellular trafficking, signaling function and ubiquitylation of membrane transporters in yeast and human cells

Techniques:•Yeast culture and molecular genetics •Western blot to study phosphorylation and ubiquitylation of proteins •Fluorescence microscopy •Human cell culture •RNAi

Molecular Parasitology

PIs: David Pérez-Morga (9854 – [email protected]), Luc Vanhamme (9758 – [email protected] ), Benoit Vanhollebeke (9761 – [email protected] ), and Étienne Pays (9759 – [email protected]),

Themes: •Host-parasite interactions in trypanosomiasis •Cell biology of trypanosomes •Apolipoprotein L function •Programmed cell death •Brain vascular development •Disease models in zebrafish

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Techniques: •Trypanosoma culture •Fluorescence microscopy •Electron microscopy •Functional assays in zebrafish •Genome editing (TALENs, CRISPRs) •Chemical and genetic screens •Analysis of transgenic mice phenotypes

Molecular Virology

PI: Carine Van Lint (9807 – [email protected])

Themes: •Retroviruses (HIV-1, BLV, HTLV-I) •Epigenetics •Transcriptional regulation •Viral latency •Chromatin structure •Signaling pathways

Techniques: •Molecular biology •Activity assays of chromatin-modifying enzymes (HDACs, DNMTs) •Gene promoter and transcription analysis (reporter gene assays, EMSA, ChIP) •ELISA •FACS •RT-qPCR •bisulfite sequencing •PBMC purification from human blood •Viral reactivation assays •MACS and RoboSep isolation •Western-blot •Viral DNA and RNA quantification •Work in BL2+ and BL3

RNA Metabolism

PI: Denis Lafontaine (9771 – [email protected])

Themes: •Molecular, cellular and pathophysiological aspects of eukaryotic ribosome biogenesis •Structure-function relationships in the nucleolus

Techniques: •RNA biochemistry (Northern blot, primer extension, metabolic labeling, RNase mapping) •q-PCR & Ch-IP •Fluorescence microscopy, including: Spin disc confocal, high-throughput and Live cell imaging •Yeast and mammalian cell culture •RNAi

Structural Biology and Biophysics

PI: Abel Garcia-Pino (6768 – [email protected])

Themes: •Structural biology •Bacterial persistence •RelA •Bacterial transcription and translation •Toxin-antitoxin modules

Techniques: •Biochemistry and biophysics (ITC, DSC, SPR, spectroscopy) •Structural biology (X-ray cristallography and SAXS)

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Scientific expertise on the campus

Institute for Medical Immunology

PIs: Michel Braun (9550 – [email protected]), Véronique Flamand (9557 – [email protected]), Stanislas Goriely (9586 – [email protected]), Alain Le Moine (9551 – [email protected]), Arnaud Marchant (9588 – [email protected]), Florence Roufosse ([email protected]), and Fabienne Willems (9584 – [email protected])

Themes: •Human immunology and vaccinology •Neonatal immunity •Gene regulation and signaling in human and animal immune responses •Transplantation

Techniques: •Flow cytometry •Molecular analysis of immune responses:

Parasitology

PI: Eric Muraille (0478/96.17.82 - [email protected], [email protected])

Themes: •Brucellosis, Leishmaniasis, Listeriosis, Escherichia coli O18:K1 and immune response against these pathogens in mouse model

Techniques: •Animal handling •ELISA, bacteria and cell culture •Immunohistofluorescence •Flow cytometry

IMI presidency: Dr. Arnaud Marchant (9588 - [email protected])

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Plant biotechnology

PIs: Mondher El Jaziri ([email protected] – 9572) and Marie Baucher ([email protected] – 9579)

Themes: •Tree development and molecular biology •Plant-microorganism interactions •Isolation of pharmacological molecules

Techniques: •Plant, bacteria and nematode culture •Genetic engineering •qPCR •Western blot

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Practical information

Practical informationAccess cardsTo obtain an access card for the parking and buildings, contact Mrs Isabelle Dubray (Infrastructure Dept., AN106, 9612 - [email protected]).

IT servicesThe Biopark IT team provides support and maintenance but also various tools. To use these, you first need to be registered by the director of your research unit. Here is how he/she can proceed:

1) Go to http://biopark-it.be/ad2) Enter login and password 3) Click on the command "Add a user to <NAME OF EQUIPE>“4) Enter the requested information (Fields preceded by a * are required)5) Validate the request by clicking the "Submit" button.

Within 24 hours, a confirmation e-mail will be sent to you, together with the procedure to activate your account.

1) File server

The file server provides storage space for your data. Regular backups are performed to ensure the continuity of data stored therein.

Once you have activated your account, you have access to three separate storage spaces:• a private directory, accessible only to you• a group directory, accessible to all the members of your team• a public directory, accessible to all the members of the institute.

Additional storage space may be provided on demand in individual cases.

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2) FTP server

To exchange large files with external people, we can create FTP storage space on demand.

3) Biopark ResSoft

ResSoft is a web application that allows you to book rooms and equipment. It is available on http://ressoft.biopark-it.be. Note that to book some instruments, you need to be authorized to do so by the person in charge.

4) Mailing lists

To receive all campus information, reminders about seminars, etc., you can subscribe to dedicated mailing lists:

• IBMM list: go to http://listserv.vub.ac.be/mailman/listinfo/newibmm or contact Mrs Marylin Boutchon (9801 – [email protected])

• IMI list: contact Mrs Laurence Vilain (9564 – [email protected])

For more information, visit our http://www.biopark-it.beintranet (accessible only from the ULB network)

or contact us by phone or email: 9589 – [email protected]

Sébastien Coinne Cédric Verbelen

We are also at your disposal in our new IBMM office (AW2.101) on Monday and Wednesday

and in our Biopôle office (D2.108) on Tuesday, Thursday, and Friday

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Practical information

The SCPPT: Service central de prévention et de protection au travailThe SCPPT deals with all aspects of the well-being of university workers and the environnment. It is part of the central administration and is based in Brussels. However, several of its members regularly pay a visit to the Gosselies campus. The SCPPT includes the following.

1) Occupational medical services

Occupational medical services ensure that workers receive health surveillance appropriate to the health and safety risks they incur at work.

2) Internal department of prevention and protec-tion at work (SIPP)

The SIPP department has two missions:

• Risk management • Prevention of fire • Prevention of accidents at work• Management of dangerous waste

In case of emergency, call the 7 from any campus phone.

Chemical waste is collected on the 2nd and the last Thursday of each month, from 2 to 3 PM, in the SIPP locale, next to the IBMM supplier entrance.

In addition to the SIPP services, Jean-Philippe Mottoul, the IT & Biosafety director of ImmuneHealth, manages biosafety for all Biopark labs. You can contact him by mail at [email protected].

Campus physician: Dr. Patricia Windey

02 555 6664 – [email protected]

For more information: http://www.ulb.ac.be/sipp

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3) Health physics (Contrôle physique)

The mission of this service is to limit the risks associated with the use of ionizing radiation.

• Attribution of a pair of dosimeters to people who work in labs using radioisotopes; monthly exchange of dosimeters, measure of the radiation dose and transmission of the results to the labs

• Installation and control of the premises dedicated to the manipulation or storage of radioactive products

• Collect of radioactive waste

4) Psychosocial service

This service provides advice with regard to professional difficulties linked to the hardship of work or to disturbed work relations. It also plays a role of conciliation in cases of conflicts or harassment complaints.

For more information: http://www.controlephysique.beContact persons are Mrs Michèle Viste and Mr Cyril Fanchon

(2096 – [email protected])

For more information: http://www.ulb.ac.be/scppt/aspectspsychosociaux.html

• Environmental protection

For more information: http://www.ulb.ac.be/environnement

Campus restaurant: le Point BarLocated in the Point-Centre building, the restaurant proposes hot meals and sandwiches from Monday to Friday. Reduced rates are granted to ULB students and employees upon presentation of their ULB card.

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Practical information

How to arrive on the campus?

1) By the ULB shuttle busses

Shuttle busses run every day except on week-ends, public holidays, and certain days during the end-of-year holidays. The operator is the Eurobussing company. Arrivals and departures from rue Adrienne Bolland, at the back of IBMM.

Brussels => Gosselies campus

• Departure from the Solbosch campus (STIB stop « ULB », avenue de l’Université) at 8h15 sharp

• Brief stops at the three following STIB bus stops: • « Cimetière d’Ixelles » (avenue de la Couronne)• « Fraiteur » (boulevard du Triomphe, in front of the Renault garage)• « Delta » (avenue de Beaulieu, bus stop shared by the STIB, TEC and

De Lijn bus companies)

• Arrival on the Gosselies campus at 9h15

Gosselies campus=> Brussels

Departure from rue A. Bolland at 18h , same stops as above in reverse order.

Luttre train station => Gosselies campus

Departure from the TEC bus stop in front of the station at 7h30, 8h30, 9h30, and 13h (direct connections with trains arriving from Brussels)

Gosselies campus => Luttre train station

Departure from rue A. Bolland at 12h30, 16h05, and 17h05 (direct connections with trains leaving for Brussels)

Local contact person for shuttle busses:

Prof. Muriel Moser

9863 – [email protected]

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2) By public transportation

The TEC bus line 68 « Sud-Ransart-Aéropôle-Sud » stops in front of the IMI at the « Gosselies U.L.B.» stop. With this line you can directly come from or go to the Charleroi-Sud train station in Charleroi. On Saturday, you may want to consider walking to or from the “Gosselies aérogare” stop.

Charleroi-Sud train station => Gosselies ULB. (about 30 min)

• Monday to Friday: ≥ 1 departure per hour, first departure at 6h03• Saturday: departures at 7h15, 8h15, 9h15, and 10h15

Gosselies ULB => Charleroi-Sud train station (15-20 min)

• Monday to Friday: ≥ 1 departure per hour, last departure at 19h22 except on Wednesday (16h42)

• Saturday: departures at 7h44, 8h44, 9h44, and 10h44

On the weekdays, another possibility is to combine the tramway M3 line (which joins the Charleroi-Sud train station to the « Jumet-Madeleine » stop) with the bus line 62 (which joins the former to the « Gosselies ULB » stop).

Charleroi-Sud train station => Gosselies ULB (about 40 min)

• Monday to Friday: departures at 7h11, 7h41, 14h41, and 15h41

Gosselies ULB => Charleroi-Sud train station

• Monday to Friday: departures at 7h45, 8h15, and 16h45

3) By car

The ULB encourages people to share a ride and is affiliated to two online carpooling platforms: http://ulb.djengo.be and http://www.carpool.be.

Verify the day’s schedule on http://www.infotec.be

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DisclaimerThis document is not an official publication of the ULB or of the Biopark Charleroi Brussels South. Neither of them can be held responsible for the accuracy of its content, which was researched by volunteers working on the Gosselies campus. For any inquiries about this guide, please contact the secretary of the Départment de Biologie Moléculaire, Mrs Marylin Boutchon.