brucelle by franchesca grepo
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THE BRUCELLAEFlorencondia, Jean Loren O.Grepo, Franchesca D.BSP3D
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Identificationand
morphologyPathogensis Diagnostic
laboratory testPharmacologic
interventionPran
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▪Brucellosis, also known as “undulant“Mediterranean fever” or “Malta fevzoonosis and the infection is
invariably transmitted by direct or icontact with infected animals oproducts – (WHO)
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Important specie
Zoonosis
Br. Melitensis- Goat
Br. Abortus- abortion in Cattle
Br. ovis- sheep
Br. suis- pigs
Br. Canis - dogs
B. neotomae desert wood rat
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Identification and Morphology
Gram negative coccobacilli
Intracellular Parasite
Strict aerobic
Non motile
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Non spore
1.2 microm
Colonies ap
in 2-5 days
Small, convcolonies
Identification and Morphology
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Transmission
Skin TransmissionAccidental penetration and abrasion: Farmers and Veterinarians
Respiratory transmission
Laboratory workers
Oral transmissionunpasteurised milk & products, raw milk or meet.
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Pathogenesis
Enters the body
Macrophage activationPolymorph migration & Phagocy
Intracellular multiplication
Res organs
blood
Any organ
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Diagnostic laboratory test A. Specimens
Bloodlymph nodes, bonefor Culture
Serumserologic test
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Diagnostic laboratory test B. Culture
Brucella Agar
Trypticase soy agar with orwithout 5% sheep blood
Brain-heart infusion medium
Chocolate agar
All cultures should be incubated in 8-10% Carbon dioxide at 35-37 degrees. Ob
urea
• positive
oxida
• posit
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Diagnostic laboratory test C. Serology
• IgG agglutinin titers above 1:80 indicateactive infection.
• If negative, presence of “blockingantibodies”
Agglutinationtest
• IgA antibodies that interfere with agglutination by IgGand IgM, cause a serologic test to be negative.Blocking
antibodies
• IgG, IgA & IgM may be detected.
• Use of cytoplasmic protein as antigen
• More sensitive and specific than agglutination testELISA Assays
Ig
• 1st wk. of acpeak at 3mopersist durinillness.
Ig
• 3 weeks after ondisease, peak atremain high durdisease
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ELISA and Serologic test
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Culturing brucella
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Treatment
▪ Susceptible to tetracyclines, rifampin, trimethoprsulfamethoxazole, aminoglycoside and some quin
▪ Combined treatment with tetracycline and estreptomycin for 2-3 weeks or rifampin for 6
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Prevention and Control
Pasteurizationof milk or milk
products
reduction ofoccupational
hazards
In laboratorymust have a
strict biosafetyprecautions
Cdda
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References
WHO.INT &&
Jawetz, Melnick & Adelberg’s
MEDICAL MICROBIOLOGY 26th e
Submitted to:
Mr. Bryan de PaduaMicrobiology & Parasitology