BRUCELLOSIS

byHASUTJI ENDAH NARUMI

DEPARTMENT OF MICROBIOLOGY; LAB OF BACTERIOLOGYFACULTY OF VETERINARY MEDICINE

AIRLANGGA UNIVERSITY

Brucellosis = Abortus Infections Disease

PRIMER attack to cow, sheep and pig

SECUNDAIR attack to other animalsand human being

COW Bang’s disease

HUMAN undulans fever (malta fever)

» Abortus» Give birth to meal» Reproduction disturbance

infertility» Milk product x» Low mortality» Treatment value» Zoonosis (important)

ECONOMIC COST

◙ - Stick- Gram (-)- Non motile- Non spora

◙ Intra seluler◙ Three species which attack to livestock

- B. militensis- B. abortus- B. suis

ETIOLOGY

Susceptible animal

EPIZOOTIOLOGY

» Cow and dog B. abortus infections abortus

» B. abortus attack horses, pigs

» B. suis pigs, dogs

» B. melitensis sheeps

Disseminating cow milk in JavaRescue institute of animal disease Bekasi, Bogor, Kediri & Jakarta Health office human which work at Denpasar

The transmission of disease

Through

Gastrointestinal tract Genitalia tract Skin, mucosa

PATHOGENESIS

Clinical symptom» Infectious abortus temporary / permanent of 5 -

8 months» Occur revers to normal» milk product» Health produce infectious vaginal liquor» Mindness of foetal liquor» Pituitary gland ≠ symptom, other side milk

contaminated againts bacteri nipple zoonosis» Epidydimitis (Male Epidydimitis) & orchitis

SYMPTOM OF THE DISEASE

‡ Thickening of placenta‡ Spreads over the chorions layers‡ Mindness of foetal of liquor, brown -

yellow, mixed with pus

2. POST MORTEM

Removal and Materials Delivery Put into ice (suggestion) Delivery to the lab Put on preserved solution

» Materials :- Milk : all nipple / not all nipples contaminated

with bacteria- Vol 20 ml < washing with alc sol 70%- 1st, 2nd out pouring should be throw among - In the cold condition

Male : Testis Inppurate Necrose

Using wire, with cotton point (absorb a thread) put the cotton in to the glass jar

Blood cultivate (from V. Jugularis)

- This area should be wash and rubbed this area using cotton talk 70%.

- Put 10 ml with spuit + heparin (anti coagulant)

Blood serologic test

Vagina : removal on 6 weeks

post partum / abortus

Placenta : infections cotyledone smears slide fictations using methanol sol

Fetus abortus :- Disposal of gatric- Pulmo- Lien- Fetal disposal- Take with sterile pasteur pipet

Carcass :- The predict of bact is different among the species

animals- Cow lgl supramamaria, lien, nipple, uterus

- Material samples- Base on :

» Clinical symptom» PA» Laboratory :

- Isolated and bacteria identification- Serologic test, blood serum thrid of mucosa vagina / milk, sperm

DIAGNOSIS

Abortus : infection camphylobacterfoetus and trichomonas foctos

C. Foetus abortus usually happenevery time

T. Foetus abortus happen earhide Brucella abortus happen after

6 months

DIFFRENTIAL DIAGNOSA

Route of administration :» to classified among

» report the sample

» to give the certificate for free Brucellosis

» to give a tag for each sample post vacc & reactor

PREVENTIVE OF THE DISEASE

Sanitation :Sanitation :» To burn the infectious material» To avoid a matting which suffer from a disease» Avoid for young animals from mother which

suffer from a disease to give milk» Crages should be disinfected to avoid suffer

from a disease» To refreshment a animals should be free from

brucellosis» To monitor the mobilitation or animals

» Standardization Dx / for Brucell (methods and reagent)

» To point out infect and healthy area from Brucellosis

» To point out infections cattles and healthy area from brucella

» To point a policy for use a vaccination» To give a certificate of health for a healthy care

ERADICATION

» Attacks animals and human

» Cause of spirochaeta bact, genus of leptospira

» The change subclinic of infectious were : abortion, hepatitis and nephritis death

Leptospirosis = Weil’s disease

F o r m : Long filament Gram (-) Aquatic saprofite apparently

ETHIOLOGY

» Resistant inside the water (1 month)» Inside the sea water, urine + rapidly

Incident in Indonesia

EPIZOOTIOLOGI

Isolation from human, dog, pig and micer / rat Serolog reactor + cow, buffalo and pig Pig at RPH Bogor, which contain bacteria

inside the ren At middle & East Java economic cost

at the cattle of pig and cow

Incident in Philippine

◙ Leptospira canicola dog ◙ Leptospira harjo cow◙ Leptospira ictero haemorrhagie mice / rat◙ Human infection through water, soil, flora which

contaminated of animal urine◙ Incubation periods 4 - 19 days

The Familiar Of Several Leptospira

» Bact body through mucosal layer multiple especially in : liver, ren, mammary gland, brain layer

» Leptospiremia 1st week post infect» Bact produce toxin and hemolysin

blood capilary disturbance tissue disturbances

» Bact grow at tubulus renalis release via urine» Bact still alive in the urine several hour at 150C (several

days at 40C)» Lethal because of septichemia, anemia, liver disorder and

uremia» State of infect depend on : age, species, bact reservoir

cause of infect.

PATOGENESIS

The Route of Disease Mechanisms

Bact inside the ren come out from urine

Cow (acute, subacute and chronic)Acute : » fever, anemia, T after 2 - 4 days

» Milk prod become normal after 2 weeks » Milk produce mixed with pus

Pig : » abortus» retentio secundinarum» + neonatal» bact vehicle for cow & human

Human Infected From Contaiminated Animals (Rats)

CLINICAL SYMPTOM

Cow : liver abses, ren coloredblack dark

Calf : icterus, liver absces, dark ren, petechial haemorrhagie,

VU contain clean red urine Chronic :absces ren, if slices contained

of liquid

POSTMORTEM DISORDER

Live animal under fever condition :- Urin without preserve- Aseptic blood + citrate- Aseptic milk and not preservative

From tetal animal- Urine, liver slices, ren, lien and blood cor

From healthy animals- Urine and serum

DIAGNOSIS

Material of Sample

Bacterial Isolation Inject animal

DIFFERENSIAL DIAGNOSIS

» Babesiosis with blood strike not effective againt of leptospirosis

» Because the symtomp is abortus until confused with the cause of the other abortus

» Clinical symptom, Hb uria, cause of clostridum, haemolyticum

Death of animal - which bring the leptospira especially rodentia mild

R/ Antibiotics (Penicilline) L. canicola- Streptomycine, Aureomycine

PREVENTION