B.Sc MICROBIOLOGY - SYLLABUS

SEMESTER - I

CORE : PAPER-I GENERAL MICROBIOLOGY

UNIT I

History of microbiology, microscopy including electron microscope; stains

and staining methods. Anatomy of prokaryotic and eukaryotic cells. Reproduction

of bacteria and fungi. Culture media and pure culture techniques. Anaerobic

culture techniques.

UNIT II

Sterilization and disinfectants; antimicrobial chemotherapy. Methods of

bacterial identification- morphological, physiological, biochemical and serological

properties.

UNIT III

Physiology of microbial growth and nutrition. Batch- continuous-

synchronous cultures; Growth Curve. Nutritional requirements. Transport of

nutrients by active and passive transport, concepts of free energy. Energy rich

bonds. Oxidative phosphorylation.

UNIT IV

Glycolysis, TCA cycle- peculiar pathways- HMP, ED, PPP, glyoxylate

pathway, glycolic acid cycle. Fermentation – lactic acid, propionic acid, acetic

acid and mixed acids.

UNIT V

Photosynthesis- oxygenic and anoxygenic. Nitrogen fixation; bio-geo

chemical cycles – metabolism during sporulation.

CORE : PAPER II- Practical -I

UNIT I

Cleaning of glass wares Sterilization principle and methods- moist heat- dry heat

and filtration methods. Media preparation: liquid media, solid media, agar slants,

agar plates, basal, enriched, selective media preparation- quality control of media,

growth supporting properties, sterility check of media.

UNIT-II

Pure culture techniques: streak plate, pour plate, decimal dilution. Culture

characteristics of microorganisms: growth on different media, growth

characteristics and description. Demonstration of pigment production.

UNIT-III

Microscopy: light microscopy, bright field microscopy, dark field

microscopy. Motility demonstration: hanging drop, wet mount preparation, dark

field microscopy, semi solid agar, Craigie’s tube method. Staining techniques:

smear preparation, simple staining, Gram’s staining, acid fast staining, staining of

Metachromatic granules.

UNIT-IV

Morphology of microorganisms: morphological variations in algae,

morphology of fungi, slide culture technique. Antibiotic sensitivity testing: Disc

diffusion test- quality control with standard strains. Micrometry: Demonstration

of size of yeast and fungal filaments.

UNIT-V

Physiology characteristics: IMViC test, H2S, Oxidase, catalase, urease test.

Carbohydrate fermentation test, maintenance of pure culture, paraffin method, stab

culture, maintenance of mold culture.

ALLIED –PAPER 1 CHEMISTRY I

UNIT I

Atomic structure and periodicity: Quantum chemistry; Planck’s quantum theory,

wave particle duality, uncertainty principle, quantum mechanical model of

hydrogen atom.

UNIT-II

Electronic configuration of atoms; periodic table and periodic properties;

ionization energy, electron affinity, electro negativity, atomic size.

UNIT III

Structure and bonding: Ionic and covalent bonding M.O. and V.B.

approaches for diatomic molecules, VSEPR theory and shape of molecules,

hybridization, resonance, dipole moment, structure parameters such as bond length

,bond angle and bond energy, hydrogen bonding, van der Waals interactions. Ionic

solids; ionic radii, lattice energy (Born-Haber Cycle).

UNIT IV

S.P and d Block Elements: Oxides, halides and hydrides of alkali and alkaline earth metals, B, Al, S,N,P and S, silicones, general characteristics of 3d elements.

UNIT-V

Coordination complexes: valence bond and crystal field theory, color, geometry

and magnetic properties.

SEMESTER II

CORE : PAPER – 3 IMMUNOLOGY & MICROBIAL GENETICS

UNIT I

History of immunology; Innate immunity, organs & cells in immune response.

Humoral & cell mediated immunity. Complement. Antigens – properties, types.

Vaccine,

UNIT II

Immunoglobulins – types, structure and functions of different classes of

IgG – theories of Antibody formation; Mechanism of immune response;

Generation of immunological diversity.

UNIT III

MHC in Mouse & Man; Immuno haematology; Hypersensitivity reactions;

Transplantation immunology; Immunological techniques – precipitation,

agglutination, CFT, RIA, ELISA, IFT, Western blot.

UNIT IV

Molecular concept of gene. Bacterial plasmids – structures & properties;

Bacteriophage – Lytic & Lysogenic cycle – phage λ . Structures of bacterial

Transposons – Types of bacterial Transposons; Mechanism of Antibiotic

resistance.

UNIT V

Gene transfer – transformation, Configuration & transduction; Gene

mapping Lac operon – trp operon. Mutations – various types & Mechanism; Ames

test, Role of mutation in evolution.

CORE : PAPER -4 – PRACTICAL-II

UNIT-I

Blood groups and typing - Coombs’s test. Precipitation reaction in Gel-

Outchelony double diffusion, Single Radial Immuno diffusion.VDRL, RPR.

Agglutination reactions: Slide and Tube methods RBC agglutination IHA, TPHA

Bacterial.

UNIT-II

Complement fixation test. Titration of amboceptor and complement

(demonstration only). Immunofluorescene, (Demonstration only), ELISA

UNIT-III

Isolation of Buffy coat, using heparin lymphocytes (T cells, B cells),

Enumeration of different cell types, Perpheral blood cell counts, absolute cell

counts.

UNIT-IV

Antibody productions in rabbits against sheep RBC and its titration

(Demonstration). Anaphylactic reactions in guinea pigs. Arthus reaction in rabbits,

(Demonstration).

UNIT-V

Skin tests, both immediate and delayed hypersensitivity reactions to egg

proteins, bacterial, fungal antigens.

ALLIED: PAPER 2 -CHEMISTRY II

UNIT I

Chemical Equilibria: Colligative properties of solution, ionic Equilibria in

solution, solubility product, common ion effect, hydrolysis of salts, pH, buffer and

their applications in chemical analysis.

UNIT II

Electrochemistry: Conductance, Kohlrausch law, Half Cell potentials, emf,

Nernst equation, galvanic cells, thermodynamic aspects and their applications.

UNIT III

Reaction Kinetics: Rate constant, order of reaction, molecularity, activation

energy, zero, first and second order kinetics, equilibrium constants (Kc, KP and

Kx) for homogeneous reactions, catalysis and elementary enzyme reactions.

UNIT IV

Thermodynamics: First law, reversible and irreversible processes, internal

energy, enthalpy, Kirchhoff’s equation, heat of reaction, Hess law, heat of

formation, Second law, entropy, free energy, and work function. Gibbs-Helmholtz

equation, Clausius-Clapeyron equation, free energy change and equilibrium

constant, Troutons rule, Third law of thermodynamics.

UNIT V

Mechanistic Basis of Organic Reactions: Elementary treatment of SN1,

SN2,E1 and E2 reactions, Hoffmann and Saytzeff rules, Addition reactions,

Markonikoff rule and Kharash effect, Diels-Alder reaction, aromatic electrophilic

substitution, orientation effect as exemplified by various functional groups.

Structure-Reactivity Correlations: Acids and bases, electronic and steric

effects, optical ad geometrical isomerism, tautomerism, concept of aromaticity.

REFERENCE BOOKS

1. Prescott, L.M., Harley, J.P. and Klein, D.A. (1999) Microbiology. McGraw Hill, New Delhi

2. Ketchum, P.A. (1984) Microbiology: Concepts and Applications. John Wiley and Sons, New York.

3. Madigan, M.T., Martinko, J.M. and Parker, J. (1999). Brock’s Biology of Micro organisms, 9th Edn. Prentice Hall, New Jersey.

4. Salle, A.J. (1992) Fundamental Principles of Bacteriology 7th Edn. Tata McGraw Hill, New Delhi.

5. Mandelstam, J., McQuillen, K. and Dawes, L. (1992) Biochemistry of Bacterial Growth, 3rd Edn. Blackwell Scientific Publications, Oxford.

6. Doelle, H.W. (1975) Bacterial Metablism. 2nd Edn. Academic Press, London.7. Moat, A.G. and Foster, J.W. (1995) Microbial Physiology, 3rd Edn. John

Wiley and Sons, New York.8. Roitt, I.M.(1988) Essential Immunology. Blackwell Scientific

Publications,Oxford.9. Jewetz, E., Melnic, J.L. and Adelberg, E.A. (2000) Review of Medical

Microbiology ,19th Edn. Lange Medical Publications, U.S.A.

10. Ananthanarayan, R. and Jeyaram Paniker, C.K. (1994) Text Book of Microbiology,6th Edn. Orient Longman, Chennai.

11. Jeyaram Paniker, C.K. (2006) Text Book of Parasitology. Jay Pee Brothers, New Delhi.

12. Alexender, M. (1977) Introduction to Soil Microbilogy. John Wiley and Sons, New York.

13. Stanbury, P.F., Whitaker, A. and Hall, S.J. (1995) Principles of Fermentation Technology, 2nd Edn. Pergamon Press, Oxford.

14. Frazier, W.C. and Westhoff, D.C. (1988) Food Microbiolog,. 4th Edn. McGraw Hill, New York.

15. Old, R. and Primrose, S.B. (1995) Principles of Gene Manipulation: An Introduction to Genetic Engineering, 5th Edn. Blackwell Scientific Publications, Oxford.

16. Freifelder, D. (1995) Molecular Biology. Narosa Publishing House, New Delhi.

17. Timbury, M.C. (1986) Medical Virology, 9th Edn. Churchill Livingstone, London.

18. Finegold, S.M. (2000) Diagnostic Microbiology, 10th Edn. C.V. Mosby Company, St. Louis

19. Jagadish Chander (1996) A Text Book of Medical Mycology. Interprint, New Delhi.

20. Greenwood, D., Slack, R.B. and Peutherer, J.F.(2002) Medical Microbiology, 16th Edn. Churchill Livingstone, London.

21. Arora, D.R. (2003) Text Book of Microbiology, 2nd Edn. CBS Publishers & Distributors, New Delhi.

22. Arora, D.R. and Arora, B.(2002) Medical Parasitology, 1st Edn. CBS Publishers & Distributors, New Delhi.

23. Dubey, R.C. and Maheswari, D.K. (2003) A Text Book of Microbiology, 1st Edn. S. Chand & Co. Ltd., New Delhi.

24. Dubey, R.C. and Maheswari, D.K. (2002) Practical Microbiology, 1st Edn. S. Chand & Co. Ltd., New Delhi

25. Sathyanarayana, U. (2002) Essentials of Biochemistry 1st Edn. Books and Allied (P) Ltd., Kolkata.

26. Deb, A.C., (1999) Concepts of Biochemistry, 1st Edn. Books and Allied (P) Ltd., Kolkata.

27. Collee, J.C., Duguid, J.P., Fraser, A.C. and Marimon, B.P. (1996) Mackie and McCartney Practical Medical Microbiology, 14th Edn. Churchill Livingstone, London.

28. Rose,A.H. (1976) Chemical Microbiology: An Introduction to Microbial Physiology, 3rd Edn.Plenum, New York.

29. Gottschalk, G. (1986) Bacterial Mrtabolosm, 2nd Edn. Springer-Verlag, New York.

30. Holt, J.S., Krieg, N.R., Sneath, P.H.A. and Williams, S.S.T. (1994) Bergey’s

Manual of Determinative Bacteriology, 9th Edn. Williams & Wilkins, Baltimore.

31. Davis, B.D., Delbecco, R., Eisen, H.N. and Ginsburg, H.S. (1990) Microbiology, 5th Edn. Harper & Row, New York.

32. Alexopolus, C.J. and Mims, C.W. (1979) Introductory Mycology, 3 rd Edn .John Wiley and Sons, New York.

33. Schmidt, G.D. and Roberts, L.S. (1981) Foundations of Parasitology, 2nd Edn, Mosby, St. Louis.

34. Tizard, R.I. (1983) Immunology: An Introduction. Saunders College Publishing, Philadelphia.

35. Kuby, J. (1994) Immunology, 2nd Edn. H.W.Freeman and Company, New York.

36. Elgert, K.D. (1996) Immunology:Understanding the Immune System. Wiley – Liss, New York.

37. Cambell, R. (1983) Microbial Ecology, 2nd Edn. Blackwell Scientific Publications, London.

38. Lynch, J.M. and Poole, N.J.(1979) Microbial Ecology: A. Conceptual Approach. Blackwell Scientific Publications, London.

39. Rheinheimer, G. (1980) Aquatic Microbilogy, 2nd Edn. John Wiley & Sons, New York.

40. Atlas, R.M. and Bartha, R. (1992) Microbial Ecology: Fundamentals and Applications, 2nd Edn. The Benjamin / Cummings Publishing Co.,Redwood City,CA.

41. Mitchell, R. (1974) Introduction to Environmental Microbiology. Prentice – Hall. Inc. New Jersey.

42. Subba Rao, N.S. (1995) Soil Microorganisms and Plant Growth. Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.

43. Paul, E.A. and Clark, F.E. (1989) Soil Microbiology and Biochemistry. Academic Press, London.

44. Subbha Rao, N.S. (1995) Biofertilizers in Agriculture and Forestry, 3rd Edn. Oxford & IBH Pub. Co. Pvt. Ltd., New Delhi.

45. Banwart, G.J. (1989) Basic Food Microbiology. CBS Publishers and Distributors, New Delhi.

46. Casida, J.E. (1968) Industrial Microbiology. Wiley Eastern, New Delhi.47. Adams, M.R. and Moss, M.O. (1995) Food Microbiology. Royal Society of

Chemistry, Cambridge.48. Winnacker, E.L. (1987) From Genes to Clones: Introduction to Gene

Technology. VCH, Weinheim.49. Glover, D.M. (1984) Gene Cloning:. The Mechanism of DNA Manipulation.

Chapman and Hall, London.50. Peppler, H.J. and Pearl Man, D. (1979) Fermentation Technology, Vol 1 & 2,

Academic Press, London.51. Brown, T.A. (1995) Gene Cloning. Chapman and Hall, London.

52. Maloy, S.R., Cronan, J.E., Jr. and David Freifelder. (1994) Microbial Genetics, 2nd Edn. Jones and Bartlett, Boston.

53. Walter Beck, J. and Davies, J.E.(1976) Medical Parasitology, 2nd Edn. C.V. Mosby Company, St. Louis.

54. Robert Desowitz (1980) Ova and Parasites. Harper and Row Publishers, New York.

55. Levanthal, R. and Cheadle, R.S. (1979) Medical Parasitology. S.A. Davies Co., Philadelphia.

56. Chatterjee (1986) Medical Parasitology. Tata McGraw Hill, Calcutta.57. Grierson, D. and Covery, S. (1989) Plant Molecular Biology, 2nd Edn.

Blackie, London.58. Luria, S.E., Darnel, J.E., Jr., Baltimore, D. and Campbell, A. (1978) General

Virology, 3rd Edn. John Wiley & Sons, New York.59. Fenner, F. and White, D.O. (1970) Animal Virology. Academic Press,New

York.60. Hayes, W. (1968) The Genetics of Bacteria and their Viruses. Blackwell

Scientific Publications, London.61. Bridge, E.A. (1994) Bacterial and Bacteriophage Genetics, 3rd Edn. Springer–

Verlag,New York.62. Lennette, E.H. (1974) Diagnostic Procedures for Viral and Rikettsial Diseases.

American Public Health Association, New York.63. Hoeprich, P.D. (1977) Infectious Diseases, 2nd Edn. Harper & Row

Publishers, New York.64. Glick B.K. and Pasternak, J.J. (1999) Molecular Biotechnology. Principles and

Applications of Recombinant DNA. ASM Press, Washington, DC. 65. Stryer, L. (1995) Biochemistry. W.H. Freeman & Co., New York.66. Ingraham, J.L. and Ingraham, C.A. (2000) Introduction to Microbiology, 2nd

Edn. Books / Cole Thomson Learning, UK. 67. Lee, J.D. (2001) Inorganic Chemistry. Blackwell Science,Oxford.68. Sony, P.L. (2000) A Text Book of Inorganic Chemistry. S.Chand & Sons, New

Delhi.69. Mathews, P. (1996) Advanced Chemistry.Cambridge University Press,

Cambridge.70. Greenwood, N.N. and Earnshaw, A. (1989) Chemistry of the Elements.Mac

Millan Publication New York.71. Cotton, F.A and Wilkinson, G. (1989) Inorganic Chemistry. John Wiley and

Sons, NewYork.72. Finar, I.L. (1986) Organic Chemistry, Vol 1 & 2, Longman, London..73. Schelegel, H.G. (1993) General Microbiology,7th Edn. Cambridge University

Press, Cambridge.74. Lehninger, A.L., Nelson, D.L. and Cox, M.M. (1993) Principles of

Biochemistry,2nd Edn. CBS Publishers, New Delhi.75. Pelczar, M.J., Jr., Chan, E.C.S and Kreig, N.R. (1993) Microbiology. McGraw

Hill, New York.76. Negi, A.S. and Anand, S.C. (1997) A Text Book of Physical Chemistry, 5th

Edn. New Delhi.77. Castellan (1996) Physical Chemistry. Macmillan India Ltd.,Chennai.78. Atkins, P.W. (1997) Elements of Physical Chemistry. Oxford University

Press,Chennai.79. Sadava, D.E.(2004) Cell Biology- organelle structure and function. Panima Publishers Corporation, New Delhi.

80. Rastogi, S.C. (2002) Cell Biology, 2nd Edn. New Age International Publishers, New Delhi

81. Powar, C.B. (2004) Cell Biology,3rd Edn.Himalaya Publishing House, Mumbai. 82. Rastogi, S.C.(2003) Cell and Molecular Biology. New Age International Publishers, New Delhi

83. Gerald Karp,(2002) Cell and Molecular Biology :Concepts and Experiments,3rd Edn John Wiley, New York.

84. Twyman, R.M.(2001) Developmental Biology- Instant Notes.Viva Books (p) Ltd. Chennai.

85. Verma, P.S. and Agarwal, V.K. (2008) Cell Biology, Genetics, Molecular Biology, Evolution and Ecology,5th Edn. S.Chand & Company Ltd., New Delhi.

86.Arora, M.P., Gurdarshan and Sandhu, S. (2004) Genetics,5th Edn. New Age International Publishers, New Delhi.

87. Raven & Johnson (1990) Biology,4th Edn. Wm. C. Brown Publishers, London.

88.Pandey, S.N. and Sinha, B.K (2005) Plant Physiology,3rd Edn.Vikas Publishing House,New Delhi 89. Mukherji, S. and Gosh, A.K.(2004) Plant Physiology .Tata McGraw Hill Publishers, New Delhi.

90. Bidwell, R.G.S. (1979) Plant Physiology. Macmillon, New Delhi.

91. Data, S.C. (1989) Plant Physiology. Central Book Depot, Allahabad.

92. Hall, D.V. and Rao, K.K. Photosynthesis. Arnold, London.

93. Jacob, W.P. (1979). Plant Hormones and Plant Development. Cambridge University Press, Cambridge.

94. Salisbury, F.B. and Ross, C.W. (1991) Plant Physiology. Wassworth Publication. Co.. Belmont.

95. Ting, I.P. (1982) Plant Physiology. Addison Wesley Publication, Phillippines

96. Postgate, J. (1987) Nitrogen Fixation,2nd Edn. Cassel, London.

97. Bonner, J. and Varner, J.E. (1979) Plant Physiology. Macmillan, New Delhi.

98. Daniel, W.W. (2005) Biostatistics; A foundation for analysis in the health sciences, 7th Edn. Jhon Wiley & sons Inc, New York. 99. Zar, J.H.(2006) Biostatistical analysis, 4th Edn. Pearson education Inc. New Jersey.

100. Garumani, N. (2004) An introduction to Biostatistics. JP publishers, Chennai.

101. Wilson, K. and Walker, J. (2002) Practical Biochemistry: Principles & Techniques, 5th Edn. Cambridge University Press, Cambridge.

102. Sundar Rao, P.S.S. and Richard, J. (2006) Introduction to Biostatistics & Research methods. Prentice-Hall of India(P) Ltd, New Delhi.

X. B.Sc. MICROBIOLOGY

NON MAJOR ELECTIVES

NON MAJOR ELECTIVE 1- CELLULAR ORGANIZATION

UNIT I Membrane structure and function: Structure of model membrane, lipid bilayer

and membrane protein diffusion, osmosis, ion channels, active transport, ion pumps,

mechanism of sorting and regulation of intracellular transport, electrical properties

of membranes.

UNIT II

Structural organization and function of intracellular organelles: Cell wall, nucleus,

mitochondria, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes, plastids,

vacuoles, chloroplast, structure & function of cytoskeleton and its role in motility.

UNIT-III

Organization of genes and chromosomes: Operon, interrupted genes, gene families,

structure of chromatin and chromosomes, unique and repetitive DNA, heterochromatin,

euchromatin, transposons.

UNIT IV

Cell division and cell cycle: Mitosis and meiosis, their regulation, steps in cell cycle, and

control of cell cycle.

UNIT-V

Microbial Physiology: Growth, yield and characteristics, strategies of cell division,

stress response.

NON MAJOR ELECTIVE 2- CELL COMMUNICATION AND CELL SIGNALING

UNIT I

Host parasite interaction: Recognition and entry processes of different

pathogens like bacteria, viruses into animal and plant host cells, alteration of host

cell behavior by pathogens, virus-induced cell transformation, pathogen-induced

diseases in animals and plants, cell-cell fusion in both normal and abnormal cells.

UNIT-II

Cell signaling: Hormones and their receptors, cell surface receptor, signaling

through G-protein coupled receptors, signal transduction pathways, second

messengers, regulation of signaling pathways, bacterial and plant two-component

signaling systems, bacterial chemotaxis and quorum sensing.

UNIT III

Cellular communication: Regulation of hematopoiesis, general principles of cell

communication, cell adhesion and roles of different adhesion molecules, gap

junctions, extracellular matrix, integrins, neurotransmission and its regulation.

UNIT-IV

Cancer: Genetic rearrangements in progenitor cells, oncogenes, tumor suppressor

genes, cancer and the cell cycle, virus-induced cancer, metastasis, interaction of

cancer cells with normal cells, apoptosis, therapeutic interventions of uncontrolled

cell growth.

UNIT V

Innate and adaptive immune system: Cells and molecules involved in innate

and adaptive immunity, antigens, antigenicity and immunogenicity. B and T cell

epitopes, structure and function of antibody molecules, generation of antibody

diversity, monoclonal antibodies, antibody engineering, antigen-antibody

interactions, MHC molecules, antigen processing and presentation, activation and

differentiation of B and T cells, B and T cell receptors, humoral and cellmediated

immune responses, primary and secondary immune modulation, the

complement system, Toll-like receptors, cell-mediated effector functions,

inflammation, hypersensitivity and autoimmunity, immune response during

bacterial (tuberculosis), parasitic (malaria) and viral (HIV) infections, congenital

and acquired immunodeficiencies, vaccines.

NON MAJOR ELECTIVE 3- DEVELOPMENTAL BIOLOGY

UNIT I

Basic concepts of development: Potency, commitment, specification, induction,

competence, determination and differentiation; morphogenetic gradients; cell fate

and cell lineages; stem cells; genomic equivalence and the cytoplasmic

determinants; imprinting; mutants and transgenics in analysis of development.

UNIT-II

Gametogenesis, fertilization and early development: Production of gametes,

cell surface molecules in sperm-egg recognition in animals; embryo sac

development and double fertilization in plants; zygote formation, cleavage,

blastula formation, embryonic fields, gastrulation and formation of germ layers in

animals; embryogenesis, establishment of symmetry in plants; seed formation

and germination.

UNIT III

Morphogenesis and organogenesis in animals: Cell aggregation and

differentiation in Dictyostelium; axes and pattern formation in Drosophila,

amphibia and chick; organogenesis – vulva formation in Caenorhabditis elegans;

eye lens induction, limb development and regeneration in vertebrates;

differentiation of neurons, post embryonic development-larval formation,

metamorphosis; environmental regulation of normal development; sex

determination.

UNIT IV

Morphogenesis and organogenesis in plants: Organization of shoot and root

apical meristem; shoot and root development; leaf development and phyllotaxy;

transition to flowering, floral meristems and floral development in Arabidopsis

and Antirrhinum.

UNIT-V

Programmed cell death, aging and senescence.

NON MAJOR ELECTIVE 4- PLANT PHYSIOLOGY

UNIT I

Photosynthesis: Light harvesting complexes; mechanisms of electron transport;

photoprotective mechanisms; CO2 fixation-C3, C4 and CAM pathways.

Respiration and photorespiration: Citric acid cycle; plant mitochondrial

electron transport and ATP synthesis; alternate oxidase; photorespiratory

pathway.

UNIT-II

Nitrogen metabolism: Nitrate and ammonium assimilation; amino acid

biosynthesis.

Plant hormones: Biosynthesis, storage, breakdown and transport; physiological

effects and mechanisms of action.

UNIT III

Sensory photobiology: Structure, function and mechanisms of action of

phytochromes, cryptochromes and phototropins; stomatal movement;

photoperiodism and biological clocks.

UNIT-IV

Solute transport and photoassimilate translocation: Uptake, transport and

translocation of water, ions, solutes and macromolecules from soil, through cells,

across membranes, through xylem and phloem; transpiration; mechanisms of

loading and unloading of photoassimilates.

UNIT V

Secondary metabolites - Biosynthesis of terpenes, phenols and nitrogenous

compounds and their roles.

Stress physiology: Responses of plants to biotic (pathogen and insects) and

abiotic (water, temperature and salt) stresses; mechanisms of resistance to biotic

stress and tolerance to abiotic stress

SECOND SEMESTER

NON MAJOR ELECTIVE 1- INHERITANCE BIOLOGY

UNIT I

Mendelian principles: Dominance, segregation, independent assortment, deviation

from Mendelian inheritance.

Concept of gene: Allele, multiple alleles, pseudoallele, complementation tests.

Extensions of Mendelian principles: Codominance, incomplete dominance, gene

interactions, pleiotropy, genomic imprinting, penetrance and expressivity, phenocopy,

linkage and crossing over, sex linkage, sex limited and sex influenced characters.

UNIT II

Gene mapping methods: Linkage maps, tetrad analysis, mapping with molecular

markers, mapping by using somatic cell hybrids, development of mapping population

in plants.

Extra chromosomal inheritance: Inheritance of mitochondrial and chloroplast genes,

maternal inheritance.

UNIT III

Microbial genetics: Methods of genetic transfers – transformation, conjugation,

transduction and sex-duction, mapping genes by interrupted mating, fine structure

analysis of genes. Human genetics: Pedigree analysis, lod score for linkage testing, karyotypes, genetic disorders. Quantitative genetics: Polygenic inheritance, heritability and its measurements, QTL

mapping.

UNIT IV

Mutation: Types, causes and detection, mutant types – lethal, conditional,

biochemical, loss of function, gain of function, germinal verses somatic mutants,

insertional mutagenesis.

UNIT VStructural and numerical alterations of chromosomes: Deletion, duplication,

inversion, translocation, ploidy and their genetic implications.

Recombination: Homologous and non-homologous recombination, including

transposition, site-specific recombination.

NON MAJOR ELECTIVE 2-DIVERSITY OF LIFE FORMS

UNIT IPrinciples and methods of taxonomy:Concepts of species and hierarchical taxa,

biological nomenclature, classical and quantititative methods of taxonomy of

plants, animals and microorganisms.

UNIT II

Levels of structural organization: Unicellular, colonial and multicellular

forms; levels of organization of tissues, organs and systems; comparative

anatomy.

UNIT III

Outline classification of plants, animals and microorganisms:Important

criteria used for classification in each taxon; classification of plants, animals and

microorganisms; evolutionary relationships among taxa.

UNIT IV

Natural history of Indian subcontinent: Major habitat types of the

subcontinent, geographic origins and migrations of species; common Indian

mammals, birds; seasonality and phenology of the subcontinent.

UNIT V

Organisms of health and agricultural importance: Common parasites and

pathogens of humans, domestic animals and crops.

NON MAJOR ELECTIVE 3- EVOLUTION AND BEHAVIOUR

UNIT I

Emergence of evolutionary thoughts: Lamarck; Darwin–concepts of variation,

adaptation, struggle, fitness and natural selection; Mendelism; spontaneity of

mutations; the evolutionary synthesis.

Origin of cells and unicellular evolution: Origin of basic biological molecules;

abiotic synthesis of organic monomers and polymers; concept of Oparin and

Haldane; experiment of Miller (1953); the first cell; evolution of prokaryotes;

origin of eukaryotic cells; evolution of unicellular eukaryotes; anaerobic

metabolism, photosynthesis and aerobic metabolism.

UNIT II

Paleontology and evolutionary history: The evolutionary time scale; eras,

periods and epoch; major events in the evolutionary time scale; origins of

unicellular and multicellular organisms; major groups of plants and animals;

stages in primate evolution including Homo.

UNIT III

Molecular Evolution: Concepts of neutral evolution, molecular divergence and

molecular clocks; molecular tools in phylogeny, classification and identification;

protein and nucleotide sequence analysis; origin of new genes and proteins; gene

duplication and divergence.

UNIT IV

The Mechanisms: Population genetics – populations, gene pool, gene

frequency; Hardy-Weinberg law; concepts and rate of change in gene frequency

through natural selection, migration and random genetic drift; adaptive radiation

and modifications; isolating mechanisms; speciation; allopatricity and

sympatricity; convergent evolution; sexual selection; co-evolution.

UNIT V

Brain, Behavior and Evolution: Approaches and methods in study of

behavior; proximate and ultimate causation; altruism and evolution-group

selection, kin selection, reciprocal altruism; neural basis of learning, memory,

cognition, sleep and arousal; biological clocks; development of behavior; social

communication; social dominance; use of space and territoriality; mating

systems, parental investment and reproductive success; parental care; aggressive

behavior; habitat selection and optimality in foraging; migration, orientation and

navigation; domestication and behavioral changes.

NON MAJOR ELECTIVE 4-METHODS IN BIOLOGY

UNIT I

Molecular biology and recombinant DNA methods: Isolation and purification

of RNA , DNA (genomic and plasmid) and proteins, different separation

methods; analysis of RNA, DNA and proteins by one and two dimensional gel

electrophoresis, isoelectric focusing gels; molecular cloning of DNA or RNA

fragments in bacterial and eukaryotic systems; expression of recombinant

proteins using bacterial, animal and plant vectors; isolation of specific nucleic

acid sequences; generation of genomic and cDNA libraries in plasmid, phage,

cosmid, BAC and YAC vectors; in vitro mutagenesis and deletion techniques,

gene knock out in bacterial and eukaryotic organisms; protein sequencing

methods, detection of post-translation modification of proteins; DNA sequencing

methods, strategies for genome sequencing; methods for analysis of gene

expression at RNA and protein level, large scale expression analysis, such as

micro array based techniques; isolation, separation and analysis of carbohydrate

and lipid molecules; RFLP, RAPD and AFLP techniques

UNIT II

Histochemical and immunotechniques: Antibody generation, detection of

molecules using ELISA, RIA, western blot, immunoprecipitation, floweytometry

and immunofluorescence microscopy, detection of molecules in living cells,

in situ localization by techniques such as FISH and GISH.

UNIT III

Biophysical methods: Analysis of biomolecules using UV/visible, fluorescence,

circular dichroism, NMR and ESR spectroscopy, structure determination using

X-ray diffraction and NMR; analysis using light scattering, different types

of mass spectrometry and surface plasma resonance methods. Radiolabeling techniques: Properties of different types of radioisotopes

normally used in biology, their detection and measurement; incorporation of

radioisotopes in biological tissues and cells, molecular imaging of radioactive

material, safety guidelines.

UNIT IV

Microscopic techniques: Visulization of cells and subcellular components by

light microscopy, resolving powers of different microscopes, microscopy of

living cells, scanning and transmission microscopes, different fixation and

staining techniques for EM, freeze-etch and freeze-fracture methods for EM,

image processing methods in microscopy.

UNIT V

Electrophysiological methods: Single neuron recording, patch-clamp recording,

ECG, Brain activity recording, lesion and stimulation of brain,

pharmacological testing, PET, MRI, fMRI, CAT . Computational methods: Nucleic acid and protein sequence databases; data

mining methods for sequence analysis, web-based tools for sequence searches,

motif analysis and presentation.

List of Courses on Soft-Skills for UG Course

S. No. Course Code Course Title Credits

1 UOM S 001ESSENTIALS OF LANGUAGE AND COMMUNICATION

2

2 UOM S 003ESSENTIALS OF SPOKEN AND PRESENTATION SKILLS

2

3 UOM S 005 PERSONALITY ENRICHMENT 24 UOM S 007 COMPUTING SKILLS 2

UOM S 001 ESSENTIALS OF LANGUAGE AND COMMUNICATION

Objectives enable students to build a repertoire of functional vocabulary and to move from the

lexical level to the syntactic level. train students to summon words, phrases relevant to the immediate communication

tasks. enable students to comprehend the concept of communication. teach students the four basic communication skills- Listening, Speaking, Reading and

Writing.

UNIT 1: Recap of language skills – vocabulary, phrase, clause, sentence.

UNIT 2: Fluency building.

UNIT 3: Principles of Communication.

UNIT 4: Types of Communication.

UNIT 5: LSRW in Communication.

Recommended Texts

Monippally, Matthukutty. M. 2001. Business Communication Strategies. 11th Reprint. Tata McGraw-Hill. New Delhi.

Sasikumar. V and P.V. Dhamija. 1993. Spoken English: A Self-Learning Guide to Conversation Practice. 34th Reprint. Tata McGraw-Hill. New Delhi.

Swets, Paul. W. 1983. The Art of Talking So That People Will Listen: Getting Through to Family, Friends and Business Associates. Prentice Hall Press. New York.

Hewings, Martin. 1999. Advanced English Grammar: A Self-Study Reference and Practice Book for South Asian Students. Reprint 2003. Cambridge University Press. New Delhi.

Lewis, Norman. 1991. Word Power Made Easy. Pocket Books. Hall and Shepherd. The Anti-Grammar Grammar Book: Discovery Activiies for

Grammar Teaching. Longman

Websites www.tatamcgrawhill.com/digital_solutions/monippally www.dictionary.cambridge.org www.wordsmith.org

UOM S 003 ESSENTIALS OF SPOKEN AND PRESENTATION SKILLS

Objectives

train students to become aware of their thinking style and to enable them to convert thinking into performance.

prepare students to evolve metal models for intra-personal and inter-personal transactions.

make students reflect and improve their use of body language – posture, gesture, facial expression, tone.

UNIT I: Thinking and Articulation – cognitive, affect, critical, creative aspects of articulation.

UNIT II: Acquisition of Oral and Aural Skills.

UNIT III: Communication Boosters – body language.

UNIT IV: Function of Cultural Codes in Presentation – etiquette.

UNIT V: Models of Presentation.

Recommended Texts

Powell. In Company. MacMillan. Cotton, et al. Market Leader. Longman. Pease, Allan. 1998. Body Language: How to Read Others Thoughts by their Gestures.

Sudha Publications. New Delhi. Gardner, Howard. 1993. Multiple Intelligences: The Theory in Practice: A Reader.

Basic Books. New York. De Bono, Edward. 2000. Six Thinking Hats. 2nd Edition. Penguin Books. De Bono, Edward. 1993. Serious Creativity. Reprint. Harper Business.

UOM S 005 PERSONALITY ENRICHMENT

Objectives

1. To make students understand the concepts and components of personality, thereby to apply the acquired knowledge to themselves and to march towards excellence I their respective academic careers.

2. To enable students to keep themselves abreast of general knowledge and current information.

3. To bring out creativity and other latent talents with proper goal setting so that self-esteem gets enhanced.

4. To sharpen memory skills and other study skills which are vital for academic excellence.

5. To give training for positive thinking which will keep the students in a good stead at the time of crisis.

Unit I- Introduction Definition of Personality Components of Personality – structural and functional aspects. Determinants of Personality- biological, psychological and socio-cultural factors. Assessment of Personality – observation, interview and psychological tests. Misconceptions and Classifications. Need for personality development.

Unit II- Self-Awareness and Self Motivation Self analysis through SWOT and Johari widow. Elements of motivation. Seven rules of motivation. Techniques and strategies for self motivation. Motivation checklist and Goal setting based on the principle of SMART. Self motivation and life.

Unit III- General Knowledge and current affairs Regional, National and International events. Geographical, political and historical facts. Information on sports and other recreational activities. Basic knowledge with regard to health and health promotion.

Unit IV- Memory, decision making and study skills Definition and importance of memory. Causes of forgetting. How to forget (thought stopping), how to remember (techniques for improving

memory) The technique of passing exams. The rational decision making process. Improving creativity in decision making and components of creativity.

Unit V- Power of positive thinking Thinking power- seven steps for dealing with doubt. Traits of positive thinkers and high achievers,\ Goals and techniques for positive thinking. Enhancement of concentration through positive thinking. Practicing a positive life style.

PRACTICAL TRAININGThe course would include the following practical exercises.Ice-breaking, Brainstorming and stimulation exercises. Thought stopping. Memory and study skills training.

REFERENCES1. Mile, D.J. (2004). Power of positive thinking. Delhi: Rohan Book Company.2. Pravesh Kumar. (2005). All about self-motivation. New Delhi: Goodwill Publishing

House.3. Dudley, G.A. (2004). Double your learning power. Delhi: Konark Press. Thomas

publishing Group Ltd.4. Lorayne, H. (2004). How to develop a super power memory. Delhi: Konark Press.

Thomas publishing Group Ltd.5. Hurlock, E.B. (2006). Personality Development, 28th Reprint. New Delhi: Tata

McGraw Hill.

UOM S 007 COMPUTING SKILLS

Objective: The major objective in introducing the Computer Skills course is to impart training for students in Microsoft Office which has different components like MS Word, MS Excel, MS Access, Power point etc., at two levels based on their knowledge and exposure. It provides essential skills for the user to get adapted to any work environment, as most of the systems in any6 work place have MS Office installed for their day to day activities. The course is highly practice oriented rather than regular class room teaching.

Pre-requisite: NIL.

Unit I: Introduction to Computers – Classification of Computers; Role of Computers in society; Inside the Computers – Hardware (processing, memory, i/o, storage), Software (systems, application), CPU, OS, (DOS, Windows, Unix, Linux), Storage Devices; Programming – Overview, need for languages, skills; Networking Basics; Virus; Hacking.

Unit II: Word Processing – Open, Save and close word document; Editing text – tools, formatting, bullets; Spell Checker; Navigating in word – keyword, Mouse; document formatting – paragraph alignment, indentation, headers and footers, numbering; printing – preview, options.

Unit III: File Management – Understanding the importance of file management; backing of files, navigating thru My Computer and Windows Explorer; Files and Folders – editing, retrieving, deleting, renaming, subfolders – manipulate windows – maximize, minimize; Power point basics – terminology, templates, viewing.

Unit IV: Spreadsheets – MS Excel – opening, entering text and data, formatting, navigating; Formulas – entering, handling and copying; Charts – creating, formatting and printing, header and footer, centering data, printing.

Unit V: Networks – Internet Explorer – components; www – working, browsing, searching, saving – Bookmark – favorite, create, delete – Printing a web page; email – creating, receiving, reading and sending messages.

Note: Unit II to Unit V needs exposure thru practicals.

References:1. Introduction to Computers – Peter Norton, Tata McGraw-Hill.1. Microsoft 2003 – Jennifer Ackerman Kettel, Guy Hat-Davis, Curt Simmons, Tata McGraw-Hill.

Examination: 1. Internal assessment could be based on Theory and/or practicals. 2. End semester is based on practicals.