but is it art? brandeis university 12.07.05. panel of artists…. and their art… will the real...
TRANSCRIPT
![Page 1: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/1.jpg)
But is it art?
Brandeis University
12.07.05
![Page 2: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/2.jpg)
Panel of artists….
and their art…
Will the real artist please stand up?
![Page 3: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/3.jpg)
![Page 4: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/4.jpg)
Genetically-encoded Oscillator
Period is ~120’ (two doublings)QuickTime™ and a
TIFF (LZW) decompressorare needed to see this picture.
Time (minutes)
![Page 5: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/5.jpg)
http://my.integritynet.com.au/purdic/oscillators.htm
Electronics 101
Hartley Oscillator Colpitts Oscillator
![Page 6: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/6.jpg)
1. Motivation
why engineer biology?
![Page 7: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/7.jpg)
1. Motivation
why engineer biology?
2. Plan
how to engineer biology?
![Page 8: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/8.jpg)
1. Motivation
why engineer biology?
2. Plan
how to engineer biology?
3. Execution
state of the art (stupid bacteria tricks)
![Page 9: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/9.jpg)
1. Motivation
why engineer biology?
2. Plan
3. Execution
… because we can
… because there’s so much to build
… because there’s so much to learn
![Page 10: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/10.jpg)
Genetically-Encoded Digital Logic
“transfer function”
time at 42°C
transcript level
3’
heat shock promoter
heat shock response gene
![Page 11: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/11.jpg)
Heat Shock Response: 32 induction
Straus, Walter, and Gross Nature (1987) 329: 347
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
![Page 12: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/12.jpg)
Genetically-Encoded Logic Functions
heat shock promoter
heat shock response gene
![Page 13: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/13.jpg)
Genetically-Encoded Logic Functions
heat shock promoter
repressor gene
repressible promoter
heat shock response gene
![Page 14: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/14.jpg)
Genetically-Encoded Logic Functions
heat shock promoter
repressor gene
repressible promoter
heat shock response gene
![Page 15: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/15.jpg)
Genetically-Encoded Logic Functions
heat shock promoter
repressor gene
repressible promoter
heat shock response geneX
![Page 16: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/16.jpg)
Logic Functions
Inverter input output
1 0
0 1
AND gate
ab
a b output
0 0 0
1 0 0
0 1 0
1 1 1
![Page 17: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/17.jpg)
Programming with DNA: hiding the details
repressor gene
repressible promoter
Genetic InverterLambda cI, P22 c2, lacI, TetR…
PRM, PR, Plac, Ptet…
![Page 18: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/18.jpg)
Programming with DNA: hiding the details
repressor gene
repressible promoter
Genetic InverterLambda cI, P22 c2, lacI, TetR…
PRM, PR, Plac, Ptet…
![Page 19: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/19.jpg)
Programming with DNA: hiding the details
Genetic Inverter
![Page 20: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/20.jpg)
Nature is source for code “Genetic Parts”
Synthetic Biology: programming with DNA
Make just about anything
Logically combined in a cell
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
http://www.nature.com/nature/comics/syntheticbiologycomic
![Page 21: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/21.jpg)
“There is a great satisfaction in building good tools for other people to use.”
-Freeman Dyson
Biology Engineering
![Page 22: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/22.jpg)
“There is a great satisfaction in building good tools for other people to use.”
-Freeman Dyson
Biology Engineering
“how can we help?”
![Page 23: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/23.jpg)
“There is a great satisfaction in building good tools for other people to use.”
-Freeman Dyson
Biology Engineering
“Cells are tiny machines”
“OK then, build me a cell”
![Page 24: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/24.jpg)
1. Motivation
why engineer biology?
2. Plan
3. Execution
![Page 25: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/25.jpg)
1. Motivation
2. Plan
how to engineer biology?
3. Execution
![Page 26: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/26.jpg)
1. Motivation
2. Plan
how to engineer biology?
3. Execution
…what foundational technologies are needed?
…are safeguards needed?
![Page 27: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/27.jpg)
Works of Art
“A good scientist is a person with original ideas. A good engineer is a person who makes a design that works with as few original ideas as possible. There are no prima donnas in engineering.”
-Freeman Dyson
![Page 28: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/28.jpg)
well characterized
predictable
modular
insulated
stable
Attributes for Foundational Technologies
![Page 29: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/29.jpg)
well characterized
predictable
modular….
insulated
stable
Attributes for Foundational Technologies
![Page 30: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/30.jpg)
http://parts.mit.edu
225 basic parts
454 composite parts
![Page 31: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/31.jpg)
http://parts.mit.edu
Standard Assembly
![Page 32: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/32.jpg)
Broadly consider two sources of risk
1. from the technology itself
2. from the people (hackers) who use it
http://biobricks.org/
G. Church, “Let us go forth and safely multiply” Nature (2005) 438:423
![Page 33: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/33.jpg)
1. Motivation
2. Plan
how to engineer biology?
3. Execution
![Page 34: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/34.jpg)
1. Motivation
2. Plan
3. Execution
state of the art
![Page 35: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/35.jpg)
1. Motivation
2. Plan
3. Execution
state of the art …SB accomplishments
…undergrad. contributions
![Page 36: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/36.jpg)
![Page 37: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/37.jpg)
Nature (2005) 434: 1130
![Page 38: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/38.jpg)
A new kind of biofilm: “coli-roid”
![Page 39: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/39.jpg)
condition lacZ result
light low light color
dark high dark color
Light Dependent Gene Expression
Phytochromes from Synechocystis
Osmoregulation system from E. coli
LacZ reporter strain from literature (1979)
![Page 40: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/40.jpg)
Light Dependent Gene Expression
![Page 41: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/41.jpg)
Biofilm v2.0
mRFP
condition mRFP result
light high red
dark low not red
![Page 42: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/42.jpg)
1. Motivation
why engineer biology?
2. Plan
how to engineer biology?
3. Execution
state of the art
![Page 43: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/43.jpg)
Three comic strips to conclude
Worst case scenario: total failure
![Page 44: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/44.jpg)
Three comic strips to conclude
Worst case scenario: total failure
Best case: total success, are we ready?
![Page 45: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/45.jpg)
Three comic strips to conclude
Worst case scenario: total failure
Best case: total success, are we ready?
Biological Engineers, an oxymoron?
![Page 46: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/46.jpg)
Credits
Tom Knight Drew Endy Randy Rettberg
![Page 47: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/47.jpg)
the end
![Page 48: But is it art? Brandeis University 12.07.05. Panel of artists…. and their art… Will the real artist please stand up?](https://reader036.vdocuments.net/reader036/viewer/2022062407/56649da05503460f94a8bbb5/html5/thumbnails/48.jpg)