Nanotech in Biomedicine:

Biodegradable Polymer Nanoparticles for drug delivery

through mucosal Membranes

By Group #3– Phillip Keller– Micheal Jones– Krista Melish– James Kancewick

Overview

• Background and Introduction– Nanotech in Biomedicine

• Problem and Basic Principles– Nano Particle Immobility/Transport Process

• Experiment and Results– Polymer Prep/Transport Characteristics

• Improvements and Future Research

Introduction

• Nanotechnology– Very Small– Very Powerful– Many Applications– Rapid Research

Growthhttp://www.redicecreations.com/ul_img/2353nanotechnology.jpg

Introduction: Biomedicine

• Biomedicine– Drug delivery– Biodiagnostics– Biosensors– Bioimaging

http://www.tlc2.uh.edu/Research/Institutes_Centers/visual_logo.jpg

Drug Delivery

• Selective Targeting– Organs– Pathogens– Cancer Cells

• Protects Deliverable– Less Medicine Wasted

• Controllable Releasehttp://web.mit.edu/newsoffice/2008/nanoparticles.jpg

Biodiagnostic

• Detection of Nucleic Acids– PCR (Polymerase chain reaction)

• Detection of Proteins– High levels of certain proteins

are indicative of various cancers and diseases

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Biosensors

http://life.nctu.edu.tw/~cslin/Tw/Research/Biosensing%20System.files/image003.gif

Bioimaging

http://www.feilab.org/pictures/facility/Flurosecence_Results.jpg

• Enhance Current Imaging Techniques– Compliment MRI/CT

– Provide contrast in previously unobtainable areas– Reduce need for exploratory surgery

Problems with Nano Particle Drug Delivery

http://www.tryingtoconceive.com/store/fern1.jpg

100X Salival Mucus

Mucus Penetration

• Cystic Fibrosis– Thickening of lung

mucosal layers

• Eye• Gut

– Drug delivery to impede cancer growth

• Female Reproductive Diseases

http://www.sciencedirect.com/science?_ob=ArticleURL

Cervicovaginal Mucus Fibers

Mucus Penetration

http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6T3R-4V4KPJ9-3&_image=fig6&_ba=6&_user

Particle Biodegradability and Drug Delivery

• No Interaction with Mucus Fibers• Dense PEGylation of particle surface allows mucus

penetration due to neutral charge distribution

http://www.sciencedirect.com/science?_ob=MiamiCaptionURL&_method=retrieve&_udi=B6T3R4V4KPJ93&_image=fig8&_ba=8&_user=952835&_coverDate=02%2F27%2F2009&_rdoc=1&_fmt=full&_orig=search&_cdi=4953&_issn=0169409X&_pii=S0169409X08002652&view=c&_acct=C000049198&_version=1&_urlVersion=0&_userid=952835&md5=be4fd7ee848e89aca12a6089c26869d2

Biodegradable Nanoparticle With Encapsulated Drug

Synthesis of PSA-PEG

•One Step Formulation Process

•Diblock Copolymers

•Covalently Bonded

Mucus-Penetrating Nanoparticle

• Dense Surface Coating of PEG

• Low Molecular Weight• Neutral Surface Charge

– Non-Penetrating particles have highly negative surface charge

Flexible Drug Delivery

•Controlled Drug Release

•Vary ratio of PEG to PSA to alter particle degradation rate

Physicochemical Properties of Nanoparticles

• Zai Potential• Surface charge characteristic

• Dw/Dm• Ratio of dispersion coefficients for particle in water

compared to human cervical-vaginal mucus

Results & Conclusions

•Rapid Transport

•Can Penetrate CVM Rapidly

Penetration in Cystic Fibrosis Sputum

•Rapid Penetration of CF Sputum

Improvements and Further Research

• Increase Particle Transfer Rates– Adapt to more specific

tissues

http://www.intel.com/technology/itj/2008/v12i3/3-paper/figures/Figure6.gif

Improvements and Further Research

• Develop Applications for a Wider Range of Diseases– Create nanoparticles capable of

accepting a wider array of drugs• Develop more chemically gentle

manufacturing processes for coating particles in PEG

http://www.labgrab.com/files/pictures/users/blogs/HIV.jpg

Improvements and Further Research

• Reduce Cost of Production– Make economically

viable• Increased targeting

reduces dosage and drug waste

http://www.maserati-rc.org/PicLib/Factory_new_production_line_3.jpg

Questions?

Resources

• Tang, Benjamin, Michelle Dawson, Samuel Lai, Ying-Ying Wang, Jung Suk, and Ming Yang. "Biodegradable polymer nanoparticles that rapidly penetrate the human mucus barrier." Proceedings of the National Academy of Sciences 106.46 (2009): 19268-9273.

• Various websites listed in slides

Rebuttal From Group U3

Micheal JonesPhillip KellerKrista Melish

James Kancewick

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What we did well

– Confidence– Graphics– Well Dressed– Basic Intro– More in depth article http://www.ipcom.co.nz/tl_files/images/Diver-Busi

nessman-web.jpg

Areas For Improvement

• Elimination of filler words• Increasing presentation time

and content• Better eye contact with the

audience• Relabeling graphics with small

words • Increase group understanding

of the articlehttp://www.ipcom.co.nz/tl_files/images/Diver-Businessman-web.jpg

Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes

Group U1

http://science-hub.com/2009/06/platinum-particles-in-the-form-of-japanese-sweets/

• The overall presentation was very good and interactive with the audience.

•The nanoparticle PSA-PEG showed to be a very effective polymer since it has good diffusion through some specific organic membranes

•Further research can be done on improving the efficiency in delivering drugs• Its still a problem to power this particles.

•We still have to do work on targeting the right receptors because in body we may have several similar mucosal membranes with similar biological and chemical properties that are healthy.

Review of Group U3’s Presentation-Review of Group U3’s Presentation-

By Group U2:By Group U2: -Kyle Demel-Kyle Demel

-Keaton Hamm-Keaton Hamm

-Bryan Holekamp-Bryan Holekamp

-Rachael Houk-Rachael Houk

http://radio-weblogs.com/0105910/ images/nanoparticles.jpg

http://dipc.ehu.es/nano2006/Nanoparticles.jpg

The presenters could have improved in the following areas:

• Presentation too short:– 30-minute presentation only lasted 15– Needs elaboration on further research:

• Delivery with viral protein coatings

• Transfer through cellular membranes

– Explain the pictures on the slides; do not just add pretty pictures because you have to

• Oral delivery – Practice speech

• Several group members were not knowledgeable on article

• Excessive use of filler words

– Work on timing and transitions

http://www.ediblecomputerchips.com/Nano-Tech/Final/Seven-A.jpg

http://news.softpedia.com/images/news2/How-Dangerous-Are-Nanoparticles-2.jpg

Nanotech in Biomedicine:

Biodegradable Polymer Nanoparticles for drug delivery through mucosal Membranes

Reviewed by Group U4

PRESENTATION/ORAL REVIEW

Overall, the speakers sounded very confident, thus enabling the audience to stay attentive to the information presented as opposed to the flaws in speech.

Excellent slides with a good balance of text and graphics. Both were large enough for the audience in the back to see clearly

Nice hands-on example with the grapefruit/lemons for further explanation of the topic.

TECHNICAL REVIEW

Perhaps this is because this research is so new, but we would have liked to have a more in depth description on how the mucus penetration would work in a variety of applications.

Also, maybe devote a little time to the future of this technology(different diseases it could fight, and different barriers the nanoparticle could help break through other than the mucus)

Overall however, good presentation that left us with few question. The questions we did have were answered well.

Nanotech in Biomedicine : Biodegradable Polymer Nanoparticles

for drug delivery through mucosal Membranes

Review of Team U3 by Team U5 – Jaynesh Shah, Greg Pudewell, Edwin L. Youmsi Pete and John Pack.

Oral and Quality of Slides Review Speakers did a good

job in the oral aspect of the presentation. They were loud and clear confident. Implying they had done a good amount of research on the topic.

The slides clear, visible and not overcrowded. Team U5 made good use of several pictures, images and graphs.

Technical Review

The presentation was satisfying from a technical standpoint.

We would have however liked them to discuss the use of biosensors in a little more details with respect to the issue of drug delivery

Presented By Group #3Phillip KellerMicheal JonesKrista MelishJames Kancewick

Critiqued by: Group U6 - Pavitra Timbalia, Michael Trevathan, Jared Walker

The slides were well balanced with graphics and text.

All presenters were well dressed and maintained an appropriate tone.

The demonstration involving the fruit to describe the receptors of drugs helped to open the presentation and get the audiences

attention. A few of the graphs were difficult to read and

understand, and were not explained carefully. The evolution leading to the development of PEG wasn’t explained until someone asked

about it during the questions section.

This presentation dealt with the delivery of drugs through the use of nano-particles engineered specifically to be able to penetrate through thick mucus that was previously not possible.

Future research needs to be done to test the particles ability to deliver drugs◦ The tests done for the paper only showed

that the particles were able to penetrate the mucus - they didn’t contain any drug payload.

This technology could help to treat many diseases such as cystic fibrosis, provided future research is done to reduce the cost of production.

Review for U3from G6

Jung Hwan Woo

• The table on the top left shows the diffusivity of the nanoparticle in mucus compared to the diffusivity in water. It is used to indicate the rate at which the nanoparticle travels in the body through mucus.

• What are the factors that affects these rates?

• What can be done to improve the particle transfer rate as suggested on the bottom left?

Jung Hwan Woo