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Chinese Journal of Tissue Engineering Research October 8, 2017 Vol.21, No.28

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ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH

4505

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He Yu-tong, Studying for

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Laboratory of Construction

and Detection in Tissue

Engineering, Guangzhou

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Province, China

Zhang Ma-hui, Master,

Department of Neurology,

Zhujiang Hospital of

Southern Medical University,

Guangzhou 510515,

Guangdong Province, China

He Yu-tong and

Zhang Ma-hui contributed

equally to this work.

Corresponding author:

Wang Le-yu, M.D., Associate

professor, Department of

Human Anatomy, Southern

Medical University,

Guangdong Provincial Key

Laboratory of Construction

and Detection in Tissue

Engineering, Guangzhou

510515, Guangdong

Province, China

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MicroRNA expression profile in the process of cyclic mechanical stretch promoting

C2C12 myogenesis

He Yu-tong

�, Zhang Ma-hui

�, Song Chen

�, Ye Gen-lan

�, Yu Lei

�, Qiu Xiao-zhong

�, Wang Le-yu

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�Department of

Human Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Construction and

Detection in Tissue Engineering, Guangzhou 510515, Guangdong Province, China;

�Department of Neurology,

Zhujiang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China)

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Abstract

BACKGROUND: In recent years, the incidence of skeletal muscle injury becomes higher and higher, but the skeletal

muscle repair ability is limited; therefore, studies on the molecular mechanism of skeletal muscle repair play a positive

role in the treatment of skeletal muscle injury.

OBJECTIVE: To explore the role of microRNA in skeletal muscle regeneration.

METHODS: C2C12 myoblasts were cyclic stretched in vitro by the Flexercell-5000 flexible device, and the appropriate

stretch condition which could induce myogenesis was selected. The microRNA expression alteration during mechanical

stretch-induced myoblast myogenesis was explored using high-throughout sequencing, and the differentially expressed

microRNAs were further studied by the bioinformatics analysis.

RESULTS AND CONCLUSION: 10% deformation, 0.125 Hz cyclic mechanical stretch could promote myoblast

proliferation and increase MyoD and Myogenin expressions in C2C12 myoblasts. MicroRNA expression profile

alteration, including the downregulated miR-500-3p/1934-5p/31-3p/378a-5p/3473b/331-3p/5097 and upregulated

miR-340-5p/449c-5p/1941-3p, were all involved in the stretch-mediated myoblast myogenesis, and the MAPK signal

pathway seemed to participate in this process. These results suggest that the low frequency of the cyclic mechanical

stretch can upregulate the expression levels of myogenic regulatory factors through the alteration of MicroRNA

expression, further inducing myogenesis, which the MAPK signal pathway may be involved in.

Subject headings: MicroRNAs; Myoblasts; Tissue Engineering

Funding: the National Natural Science Foundation of China, No. 31100700; the Guangdong Provincial Medical

Research Foundation, No. A2015412

Cite this article: He YT, Zhang MH, Song C, Ye GL, Yu L, Qiu XZ, Wang LY. MicroRNA expression profile in the

process of cyclic mechanical stretch promoting C2C12 myogenesis. Zhongguo Zuzhi Gongcheng Yanjiu.

2017;21(28):4505-4511.

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c2c12 microrna

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