GEt 25% OFF yOur FIrst OrDEr!Reference promotion code JTHGHR25 when placing your order. Offer valid through December 31, 2011.

INtrODuCtOry DIsCOuNt

Inhibitorselect™ signaling Pathway Panels

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

small Molecules, Inhibitors, and Biochemicals

What’s New from Calbiochem®

NOVEMBEr 2011

Small molecules offer a powerful approach to temporally and spatially modulate the activity of individual proteins that control biological processes. This includes identifying signaling molecules that define and maintain the extensive intracellular communication networks controlling critical cellular functions.

Researchers traditionally perform signal transduction analysis as a linear path, restricting to a specific pathway in an isolated cell, but more recently, have undertaken more of a “systems” approach, involving dynamic interaction networks. Such dynamic analysis integrates high throughput molecular profiling, database and literature mining, mechanistic modeling, and cell culture experiments.

Recognizing the opportunities and challenges in analyzing signal transduction, EMD Millipore, has developed an array of Signaling Pathway Inhibitor Panels that contain carefully selected, potent small molecule inhibitors in convenient, cost effective formats. These panels allow the researcher to modulate their pathway of interest and gain useful information by manipulating specific proteins or families.

Calbiochem®: Publish or Perish

Nucleus

Off-State On-State

Frizzled Frizzled Frizzled Frizzled

• Casein Kinase IIInhibitor III, TBCA

• LY 294002 • PP2

• U0126

• SB 202190

• Rapamycin

• PKG Ia Inhibitor,Cell-Permeable

• K-252a, Nocardiopsis sp.

• KN-93; K-252a, Nocardiopsis sp.

• JNKInhibitor

• H-89, Dihydrochloride

• (5Z)-7-Oxozeaenol,Culvaria sp.

• GSK-3 Inhibitor IX

• Casein Kinase I Inhibitor, D4476

• K-252a, Nocardiopsis sp.• H-89, Dihydrochloride

• β-Catenin/Tcf Inhibitor, FH535

NFAT

CaderinCer

Caderin

APCAXIN

DSH TAB DSH

Rac1

α-Cateninβ-Catenin

β-Catenin

β-Catenin

GSK-3β

CK1 CKII

GSK-3β

TAK1 PI3-K

Akt

Src

ERKS MAP3Ks

CaMKII

PLCMEKKS

p38

CalnPKC

PDE

PKG

MAPKK4/7Rho Kinase

JNK

mTORNLKPKA

Proteasome

ERK Pathway

CellPolarity

Cell Survival

NFATPathway

PKCPathway

MAPKCascade

Cell Survival/Protein Synthesis

Gene Expression Gene ExpressionCell Fate Proliferation,Differentiation,

Adhesion, and Survival

APCAXIN

β-Catenin

RhoA

β-Catenin

β-Catenin

β-Catenin Degradation

Ca2+

TCF/LEFCREB

ATF2

c-Myc

WNTWNT5A

WNT1/11 WNT5

G Proteins

EMD Millipore offers the Calbiochem® brand of high quality small molecules that have been cited in thousands of peer-reviewed publications. From libraries and pathway panels to individual small molecules, the Calbiochem® line of products offers the widest and most cited selection of inhibitors and activators worldwide.

New! Wnt signaling Pathway Panel and small Molecules The Wnt signaling pathway is evolutionarily conserved and involved in fate specification, developmental cell proliferation, cell migration, and polarity. Wnt genes encode a large family of secreted, cysteine-rich proteins that are important in the development and maintenance of adult tissues. See inside for details.

Featuring

NOV2011

Nucleus

Off-State On-State

Frizzled Frizzled Frizzled Frizzled

• Casein Kinase IIInhibitor III, TBCA

• LY 294002 • PP2

• U0126

• SB 202190

• Rapamycin

• PKG Ia Inhibitor,Cell-Permeable

• K-252a, Nocardiopsis sp.

• KN-93; K-252a, Nocardiopsis sp.

• JNKInhibitor

• H-89, Dihydrochloride

• (5Z)-7-Oxozeaenol,Culvaria sp.

• GSK-3 Inhibitor IX

• Casein Kinase I Inhibitor, D4476

• K-252a, Nocardiopsis sp.• H-89, Dihydrochloride

• β-Catenin/Tcf Inhibitor, FH535

NFAT

CaderinCer

Caderin

APCAXIN

DSH TAB DSH

Rac1

α-Cateninβ-Catenin

β-Catenin

β-Catenin

GSK-3β

CK1 CKII

GSK-3β

TAK1 PI3-K

Akt

Src

ERKS MAP3Ks

CaMKII

PLCMEKKS

p38

CalnPKC

PDE

PKG

MAPKK4/7Rho Kinase

JNK

mTORNLKPKA

Proteasome

ERK Pathway

CellPolarity

Cell Survival

NFATPathway

PKCPathway

MAPKCascade

Cell Survival/Protein Synthesis

Gene Expression Gene ExpressionCell Fate Proliferation,Differentiation,

Adhesion, and Survival

APCAXIN

β-Catenin

RhoA

β-Catenin

β-Catenin

β-Catenin Degradation

Ca2+

TCF/LEFCREB

ATF2

c-Myc

WNTWNT5A

WNT1/11 WNT5

G Proteins

Inhibitorselect™ Wnt signaling Pathway Inhibitor Panel (Catalogue No. 681666)

EMD Millipore’s Calbiochem® Wnt Pathway Panel contains 15 carefully selected, cell-permeable, potent small molecules to

help determine specific gene and protein functionality along Wnt signaling pathways.

FEAturED sIGnALInG PAtHWAy PAnEL

Phosphorylation

Ubiquitin

GTP

Calbiochem® InhibitorsFor more information visit www.emdbiosciences.com/inhibitorselect

nEW PrODuCts

Description Catalogue No.

Bax Channel Blocker II, iMAC3 196806

Caspase Modulator I, 1541 264157

DAPK Inhibitor 324788

Meriolin 2 445820

p53-Snail Binding Inhibitor, GN25 506170

PKCθ/δ Inhibitor 539649

RNAi Enhancer, Enoxacin Sodium Salt 557305

Description Catalogue No.

ACC2 Inhibitor, CD-017-0191 104551

Adipogenesis Inducers Panel 121000Autotaxin Inhibitor I, S32826 189510Autotaxin Inhibitor II, HA130 189511Dual Specificity Protein Phosphatase 1/6 Inhibitor, BCI 317496

Glucagon-Like Peptide 1 Receptor Agonist II 346007

GSK-3 Inhibitor IV, SB-216763 361566

GSK-3 Inhibitor XXII, Compound A 361563

GSK3β Inhibitor XIX, IM-12 361565HDL Receptor SR-BI Inhibitor, BLT-1 373210Hexokinase II Inhibitor II, 3-BP 376817

InhibitorSelect™ FGF Signaling Pathway Inhibitor Panel 341612

InhibitorSelect™ IGF Signaling Pathway Inhibitor Panel 407249

Phosphatase Inhibitor Cocktail Set III, Lyophilized  524631

PI 3-K inhibitor IX, PIK-90 528117

PI 3-K Inhibitor XI, HWT 528119

PI3Kδ Inhibitor, SW30 526559

PTP LYP Inhibitor 540217

SIRT2 Inhibitor II, AK-2 566331

Description Catalogue No.

CFTR Inhibitor IV, PPQ-102 219677

CFTR-F508del Corrector II, Corr-4a 219673

Description Catalogue No.

Aminopeptidase N/CD13 Inhibitor  164606

APT1 Inhibitor, palmostatin B 178501

Autotaxin Inhibitor I, S32826 189510

Bax Channel Blocker II, iMAC2 196806

Bcr-abl Inhibitor III, GNF-5 197224

BCRP Inhbitor  197226

c-Met Kinase Inhibitor III 448105

c-Myc Inhibitor II 475957

C-Raf Inhibitor 475958

Caspase-3 Activator, BETT 235428

CDK Inhibitor II, LGR1406 219501

Ceramide Kinase Inhibitor II, NVP-231 219493

DAPK Inhibitor 324788

DNA Methyltransferase Inhibitor II, SGI-1027 260921

Dual Specificity Protein Phosphatase 1/6 Inhibitor, BCI 317496

Dvl-PDZ Domain Inhibitor II 322338

ESFTs Inhibitor, YK-4-279 330000

Fibroblast Growth Factor-2 Inhibitor, NSC37204 341598

Formin FH2 Domain Inhibitor, SMIFH2 344092

GAG Agonist, Adhesamine 362331

Glucagon-Like Peptide 1 Receptor Agonist II 346007

HDAC Inhibitor XXIV, OSU-HDAC-44 382181

Hesperadin 375680

Hexokinase II Inhibitor II, 3-BP 376817

Hh Signaling Pathway Modulators 373386

HIF Prolyl Hydroxylase Inhibitor 400084

HIF-Hydroxylase Inhibitor, DMOG 400091

Histone Acetyltransferase p300 Inhibitor, C646 382113

HMTase Inhibitor II, Chaetocin 382191

IM-412 401251

InhibitorSelect™ TGF-βR and BMPR Signaling Pathway Inhibitor Panel

616466

InhibitorSelect™ VEGF Signaling Pathway Inhibitor Panel 676502

JAK3 Inhibitor VII, AD412 420145

JMJD2 Inhibitor, 5-carboxy-8HQ 420201

MDMX Antagonist, SJ-172550 444155

Meriolin 3 445821

Mitaplatin 475851

MRP1 Inhibitor II, Reversan 475730

Neurotrophin Antagonist, Y1036 480870

NF-κB Activation Inhibitor VII, CID-2858522  480457

Nicotinamide Phosphoribosyltransferase Inhibitor, FK866 481908

Notch Transcription Factor Inhibitor SAHM1 491002

p53 Activator VII, STIMA-1 506168

PAD3 Inhibitor, Cl4-amidine 506281

PARP Inhibitor XV, UPF-1035 528825

Phosphatase Inhibitor Cocktail Set III, Lyophilized  524631

PI 3-K Inhibitor IX, PIK-90 528117

PI 3-K Inhibitor XI, HWT 528119

Description Catalogue No.

TNAP Inhibitor 613810

Apoptosis

Diabetes, Obesity, and Cardiovascular Disease

Cystic Fibrosis

Cancer

Bone Disorders

NOV2011

nEW PrODuCts

Description Catalogue No.

HDAC Inhibitor XXIV, OSU-HDAC-44 382181

Histone Acetyltransferase Inhibitor IV, CPTH2 382111

Histone Acetyltransferase p300 Inhibitor, C646 382113

HMTase Inhibitor II, Chaetocin 382191

LSD1 Inhibitor 489476

LSD1 Inhibitor II, S2101 489477

PAD3 Inhibitor, Cl4-amidine 506281

Protein Lysine Methyltransferase Inhibitor, UNC0321 476496

SIRT2 Inhibitor II, AK-1 566331

Description Catalogue No.

Dkk-1 Inhibitor, WAY-262611 317700

Dvl-PDZ Domain Inhibitor II 322338

GAG Agonist, Adhesamine 362331

Hesperadin 375680

Hh Signaling Pathway Modulators 373386

InhibitorSelect™ TGF-βR and BMPR Signaling Pathway Inhibitor Panel

616466

LSD1 Inhibitor 489476

LSD1 Inhibitor II, S2101 489477

Notch Transcription Factor Inhibitor SAHM2 491002

Pluripotin 540020

Protein Lysine Methyltransferase Inhibitor, UNC0321 476496

Pyrintegrin 544049

Rho Kinase Inhibitor VII 555556

Rho Kinase Inhibitor XI, Y-39983 555561

Tankyrase1/2 Inhibitor, XAV939 575545

V-ATPase Inhibitor, KM91104 672999

Wnt Antagonist III, Box6 681673

Wnt Antagonist III, CCT036477 681674

Description Catalogue No.

Cathepsin L Inhibitor, CAA0225 219502

γ-Secretase Inhibitor XXIII, Aβ42-Selective 565792

GSK-3 Inhibitor IV, SB-216763 361566

GSK-3 Inhibitor XXII, Compound A 361563

GSK3β Inhibitor XIX, IM-12 361565

LRRK2-IN-1 438193

Tau Aggregation Inhibitor, ATPZ, Cpd16 580222

Epigenetics

stem Cell Biology

neurodegeneration

Cancer (continued)Description Catalogue No.

PI 3-K/PDK-1 Inhibitor, NVP-BAG956 528121

PI3Kδ Inhibitor, SW30 526559

Polo-like Kinase Inhibitor IV, SBE13 528281

PP1 Analog III, 3-MB-PP1 529582

Proteasome Inhibitor X, PI-083 539187

PTP LYP Inhibitor 540217

Pyrintegrin 544049

Rac1 Inhibitor II 553511

RNAi Enhancer, Enoxacin Sodium Salt 557305

ROS Inhibitor, YCG063 557354

RXRα Inhibitor, K-80003 557451

Tankyrase1/2 Inhibitor, XAV939 575545

Topoisomerase IIα Inhibitor, TSC24 614852

Ursolic Acid  672315

USP7 Inhibitor, HBX 41,108 662084

Wnt Antagonist III, Box5 681673

Wnt Antagonist III, CCT036477 681674

NOV2011

Wnt Antagonist III, Box5 (Catalogue No. 681673)

A Wnt-5A-derived, N-terminally butyloxycarbonyl- (Boc) protected, hexa-peptide that effectively antagonizes Wnt-5A-

mediated cellular activities in A2058 and HTB63 melanoma cultures, including migration, invasion, calcium response, and

MARCKS phosphorylation. Replacing the N-terminal Boc with a formyl modification results in a peptide termed Foxy5 that

mimics, instead of antagonizes, Wnt-5A biological activity in a Frizzled-5- (Fzd-5/Frz-5) dependent manner. The peptide

sequence is conserved in multiple species, including human, mouse, and rat, and Box5 is expected to exert its effect via

direct FZD5 binding as is the case with Foxy5.

Wnt Pathway Inhibitor VI, Boc-MDGCEL-OH, Frizzled-5 Antagonist

Dvl-PDZ Domain Inhibitor II (Catalogue No. 322338)

A cell-permeable amidobenzanilide compound that disrupts Fz-Dvl (frizzled-dishevelled) interaction by targeting the PDZ

domain (Kd = 10.6 µM) of Dvl, blocking Wnt3A-induced (10 ng/mL) transcription activity (~50% inhibition at 3 µM in

293 cell SuperTOPflash reporter assays) and suppressing the Wnt pathway-dependent growth of prostate cancer PC-3

cells (by 16% in 72 h at ≥50 µM; IC50 = 12.5 µM). Shown to effectively prevent Wnt3A mRNA injection-induced xenopus

embryo deformation (effective conc. = 25 µM) and suppress Wnt pathway-mediated apoptosis of the hyaloid vescular

endothelial cells in the mouse eyes via vitreous injection (144 fmol/120 nL/eye) in vivo.

2-((3-(2-Phenylacetyl)amino)benzoyl)amino)benzoic acid, 3289-8625, Wnt Pathway Inhibitor V

ADDItIOnAL FEAturED PrODuCts FOr tHE InVEstIGAtIOn OF Wnt sIGnALInG

For more information visit www.emdbiosciences.com/inhibitors

O

O

HN N

HCO2H

H3C

t-boc-

CH3

CH3

CH3

CH3

CH3

0

O O

O

O

OO

O

O

OH

OHOH

SH

S

HN H

N

NH N

H

NH N

H

EMD MIllipore, the M logo, and InhibitorSelect are trademarks, and Calbiochem is a registered trademark of Merck KGaA, Darmstadt, Germany.Lit No. PR4100EN00 LS-SBU-11-04938 Printed in the USA. 10/2011. © 2011 Millipore Corporation. All rights reserved.

to Place an Order or receivetechnical AssistanceIn the U.S. and Canada, call toll-free 1-800-645-5476

For other countries across Europe and the world, please visit www.millipore.com/offices

For Technical Service, please visit www.millipore.com/techservice

Get Connected!Join EMD Millipore Bioscience on your favorite social media outlet for the latest updates, news, products, innovations, and contests!

facebook.com/EMDMilliporeBioscience

twitter.com/EMDMilliporeBio