Anti-fungal of Marigold (Calendula ofcinalis) fower extractI INTRODUTION!ac"ground of t#e $tud%From hundreds of centuries ago, in ancient times, and from timeswhen the human being was active as today on the scene of life andfghting for life, along with his struggles to provide food and clothing,hewasthinkingabout hishealth, fndingwaystolivehealthyandrelieving physical ailments and illness which was later called themedicine and of course the herbal medicine which is now applicable forus.Flowers always make people better, happier, and more helpful;they are sunshine, food and medicine for the soul. A popular gardenplant, Marigold has been valued for many centuries for its' eceptionalhealingpowers andis particularly remarkableinthetreatment ofwounds. !hen used for medicinal purposes, it is commonly referred toas '"alendula'. #$unset !estern %arden &ook'(he )marigold* #Calendula ofcinalis' also called )ruddles*,common marigold, garden marigold, +nglish marigold, or $cottishmarigold is a plant in the genus "alendula of the family Asteraceae. ,tis probably native to southern +urope, though its long history ofcultivation makes its precise origin unknown, and it may possibly be ofgardenorigin. ,t is alsowidelynaturali-edfurther northin+urope#north to southern +ngland' and elsewhere in warm temperate regionsof the world.)Marigold*iswell knownasawound.healing, antisepticandstimulating remedy, but in fact modern herbalists rely upon this plantfor a wide rangeof uses. /nly Marigold's 0ower heads are usedmedicinally. (he bright petals contain powerful natural chemicals thatgivetheherbits' ama-ing healingproperties,soalwaysensurethatyou use the most vibrant orange 0owers available. Mindy %reen in herbook "alendula, has compiled an impressive list of some of Marigold'sother potential actions, includingalterative, analgesic, anthelmintic,anti.in0ammatory, antispasmodic, aperient, astringent, bactericide,carminative, styptic and tonic. Marigold has a long history of medicinaluse, stretching that one of its e1ective uses is to treat skin allergieswhich is the focus of the study in ensuring the fast cure of the ointmenton the skin of the a1ected organisms particularly dogs.)Marigold*isagenusof about 23to34speciesof annual orperennial herbaceous plants in the daisy family Asteraceae, native totheareafromMicronesia+ast throughtheMediterraneanregionto,ran. "alendula should not be confused with other plants that are alsoknown as marigolds, such as corn marigold, desert marigold, or marshmarigold or plants of the genus (agetes.#http566hmt7ournals.com6vol383629'Allergic skin reactions are very common, and it can be di:cult tofgure out what causes them. (here are hundreds of di1erent kinds ofrashes that can be caused by many things, such as plants like poisonivy, allergic reactions to a medication or a food, or a response to anillness. #;aniel More, M;'Allergic ec-ema #atopic dermatitis' and hives #urticarial' are twoof themost commonskinrashes. +c-emaa1ects24percent to34percent of children and 2 percent to 9 percent of adults. ,f you haveec-ema, your skinmaybecomered, irritatedanditchy. $ometimesthere are small, 0uid.flled bumps that oo-e. ear.roundor seasonal itchingislikely due to allergies. $igns of allergies often start as itching, and thefrst signs may be to chew, bite, scratch or lick. ?ed skin and hair lossarecommon. &lackenedandthickenedskinthatlookslikeelephantskin is symptoms of a long duration of uncontrolled disease. ,nfectionsfrombacteriaandyeast arecommonwithallergiesastheimmunesystemisnot respondingproperlytofght theorganisms normallyfound on the skin. ,n addition, dogs that chew, bite, scratch and lickdisturb the top layer of skin that protects against infection. @icking andchewingcan also lead to a warmmoist environment,which bacteriaand yeast thrive in.+perimental animalAs models are one of the best strategies forthe understanding of pathophysiology of any disease in order to designanddevelopthedrugsfor itstreatment. =umerousanimal modelshavebeendevelopedfor the past fewdecades for studyingskinallergies and testing for the anti.itchy./n this premise, this research was undertaken to determine thepossible therapeutic e1ects of )marigold* #Calendula ofcinalis'etractsonallergicdogs. ,ftheresultofthestudyisfavorable, thiscouldgiveacheaperalternativefor thetreatment of skinallergiesinstead of buying epensive medicines.(he importance of allergy testing is to rule out possible allergens,especially when multiple allergens are present in daily life. ,t makes nosensetobeginavoidingonepotential allergenwhenthereactionisactually caused by something entirely di1erent.O&'ecti(e)(his study primarily aims to test the Antifungal Activities ofMarigold #Calendula ofcinalis) 0ower +tract.$pecifcally, it aims to52 (oproduceanalternativesourceof skinointmentfromtheetract of marigold 0owers.3 (o reduce body scars with the use of marigold etract.9 (odi1erentiatethee1ectivityofacommerciali-edointmentwith that of an alternative one.I*+ortance of t#e $tud%(he researcher came up with the interest to focus on thee1ectivity of Marigold etract on the reduction of Mold growth. Atpresent the cost of synthetic drugs is still high and it continues torise causing burden for allergic organisms considering the fact thatthisallergiesreBuiresserioustreatment. (hus, thereisaneedtoseekforthetreatmentofsuchdiseasetodecreasethemortalityand morbidity due to simple skin allergies. $co+e and Deli*itation of t#e $tud%(his study will be delimited to the e1ectivity of thephytochemical components of Marigold #Calendula ofcinalis'etracts in dogs su1ering from skin allergies.(odeterminetheanti.allergice1ectsof the0ower,Marigold etract is applied to the allergic dog together with theapplication of the commerciali-ed ointment to a respondent withthe same situation.(he materials and the plant are readily available assuringto produce cheaper ointment in the market.,lace and Ti*e of t#e $tud%(hephytochemical analysisofthe)marigold*#Calendulaofcinalis'etractwasconductedatthe,ndustrial (echnology;evelopment ,nstitute.;epartment of $cienceand(echnology,Crdaneta "ity, on April D, 3429 with the technical assistance of,(;, research specialists.R-.I-/ O0 1IT-RATUR-(he Elant Material.)Marigold*. Calendula ofcinalis!otanical De)cri+tion)Marigold*.Calendula ofcinalisalso calledruddles* or )spottishmarigoldisaplantinthegenus"alendulaofthefamilyAsteraceae. ,tisprobablynativetosouthern+urope, thoughitslonghistoryof cultivationmakes its precise origin unknown, and it may possibly be of garden origin. ,tis also widely naturali-ed further north in +urope #north to southern +ngland'and elsewhere in warm temperate regions of the world.Marigold* is widely cultivatedandcanbegrowneasily insunnylocations in most kinds of soils. Although perennial, it is commonly treated asan annual, particularly in colder regions where its winter survival is poor, orinhot summer locations whereit alsodoes not survive. "alendula areconsideredbymanygardeningeperts as amongtheeasiest andmostversatile0owerstogrowinagarden, especiallysincetheytoleratemostsoils. ,n temperate climates, seeds are sown in spring for blooms that lastthroughout the summer and well into the fall. ,n areas of little winter free-ing#C$;A -onesF.22',seeds are sownin autumn for winter color,plants willwither in subtropical summer. $eeds will germinate freely in sunny or half.sunny locations, but plants do best if planted in sunny locations with rich,well.drained soil. Eot marigolds typically bloom Buickly from seed #in undertwo months' in bright yellows, golds, and oranges.#http566en.wikipedia.org6wiki6"alendula8o:cinalis'#e*i)tr%(his study aims toBuantify theactivebiological compounds in C.ofcinalis 0owers. &ased on the active principles and biological properties ofmarigolds 0owers reported in the literature, we sought to obtain andcharacteri-e the molecular composition of etracts prepared using di1erentsolvents. (heantioidant capacities of etracts wereassessedbyusingspectrophotometrytomeasurebothabsorbanceof thecolorimetric freeradical scavenger3,3.diphenyl.2.picrylhydra-yl #;EE