cama-1mda-mb-361 (her2+)hcc-1569 (her2+)bt474 (her2+)hs578tmda-mb-415...

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  • Densitometry UnitsSubtypeLMW-E LevelsABp=0.003Supplemental Figure 1Full Length Cyclin E Levels

  • Supplemental Table 1

    ProteinFC:Senp-valueI3C SensitiveCaspase 3 (active)1.73.31E-08p531.61.77E-10S6 pS235/pS2361.30.02p211.30.000111AMPK pT1721.30.00043Smac-1.20.0164E-BP1 pS65-1.26.38E-09PRAS40 pT246 -1.20.000488eEF2K-1.32.52E-06B-Raf-1.3

  • Supplemental Table 2

    I3C Sensitive Cellsp-valueNRF2-mediated Oxidative Stress Response2.00E-10Cell Cycle: G2/M DNA Damage Checkpoint Regulation2.14E-08Protein Ubiquitination Pathway1.07E-07Role of BRCA1 in DNA Damage Response3.55E-07Molecular Mechanisms of Cancer1.20E-06PI3K/AKT Signaling1.45E-06Role of CHK Proteins in Cell Cycle Checkpoint Control1.78E-06p53 Signaling2.40E-06Actin Nucleation by ARP-WASP Complex5.75E-06Mitotic Roles of Polo-Like Kinase1.00E-05ATM Signaling1.00E-05Purine Metabolism1.20E-05Aminoacyl-tRNA Biosynthesis3.31E-05Breast Cancer Regulation by Stathmin13.55E-05Hereditary Breast Cancer Signaling3.63E-05Fc Receptor-mediated Phagocytosis in Macrophages and Monocytes3.98E-05Apoptosis Signaling5.50E-05Inositol Phosphate Metabolism6.03E-05Glioma Signaling7.94E-05Rac Signaling9.55E-05Pancreatic Adenocarcinoma Signaling1.12E-04Aryl Hydrocarbon Receptor Signaling1.23E-04Induction of Apoptosis by HIV11.29E-04Estrogen Receptor Signaling1.35E-04Cell Cycle Control of Chromosomal Replication1.78E-04

    I3C Insensitive Cellsp-valueNRF2-mediated Oxidative Stress Response7.76E-0714-3-3-mediated Signaling1.15E-05Valine, Leucine and Isoleucine Degradation1.51E-05Integrin Signaling2.95E-05Fc Receptor-mediated Phagocytosis in Macrophages and Monocytes6.03E-05IL-8 Signaling7.76E-05VEGF Signaling9.77E-05One Carbon Pool by Folate1.58E-04Virus Entry via Endocytic Pathways1.95E-04Rac Signaling2.24E-04Axonal Guidance Signaling2.75E-04Actin Nucleation by ARP-WASP Complex2.82E-04Role of Tissue Factor in Cancer3.16E-04HER-2 Signaling in Breast Cancer3.16E-04Actin Cytoskeleton Signaling3.47E-04Signaling by Rho Family GTPases3.55E-04Thrombin Signaling4.17E-04Docosahexaenoic Acid (DHA) Signaling4.57E-04Breast Cancer Regulation by Stathmin15.50E-04Glioma Signaling5.50E-04Mechanisms of Viral Exit from Host Cells6.46E-04Cell Cycle: G2/M DNA Damage Checkpoint Regulation7.08E-04PI3K/AKT Signaling7.41E-04Macropinocytosis Signaling8.32E-04Germ Cell-Sertoli Cell Junction Signaling1.07E-03

  • Supplemental Figure 2MDA-MB-157BControlshAHR.1shAHR.2ActinAhRControl sh-AHR.1 sh-AHR.2 D 100 250 D 100 250 D 100 250 MA

    IC50 (M) Control490.8shAHR.1585.0shAHR.2570.0

  • Normalized Absorbance (590 nM)Log Tamoxifen concentration (M)ANormalized Absorbance (590 nM)Log I3C concentration (M)BParentalTAM-RSupplemental Figure 3

    IC50 (M) Parental0.9TAM-R5.2

    IC50 (M) Parental277.9TAM-R253.1

  • T47DZR75-1MCF-7MDA-MB-436MDA-MB-231MDA-MB-157I3C (M)p53Actin - + - + - + - + I3C (250 M) Control sh1 sh2 sh3 MCF-7ABp53CMCF-7Supplemental Figure 4ControlshP53.1shP53.2shP53.3p53 shRNA

    IC50 (M) Control265.2shP53.1254.2shP53.2243.8shP53.3222.3

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