catalogo alpco

Scientific Solutions for Life

Product Catalog

American Laboratory Products Company

(ALPCO) was founded in 1991 as an importer

and distributor of immunoassay based

products for the North American life science

markets. Our origin began through an exclusive

partnership with a European manufacturer,

Bühlmann Laboratories, located just outside

of Basel Switzerland. Over the past two

decades, ALPCO has become a premier channel

representing over 60 collaborating partners

from around the globe. In late 2008, we

launched our organically developed product

line focused in the area of diabetes and obesity

research, while also remaining true to our

roots by continuing to provide “Immunoassays

Beyond the Ordinary.”

As ALPCO has evolved, we’ve expanded our

offering into applications for HPLC, LC-MS/

MS, purified antibodies, recombinant proteins

and most recently flow cytometry based

reagents. This next year will prove exceptionally

productive as our offering will continue to

grow by over 400 new products, with a specific

development focus in our new STELLUX line

of chemiluminescent based detection systems.

While our vision has expanded to serve a

broader segment of life science research and

healthcare professionals dedicated to improving

patient outcomes, our mission to deliver

“Scientific Solutions for Life” remains the same.

- Sean G. Conley, President

Allergy

Bone Metabolism

CVD & Oxidative Stress

Diabetes & Obesity

Endocrinology

Gastroenterology

Immunology

Neurobiology

Toxicology

Flow Cytometry

Accessories & Equipment

Index

Ordering Information

4

20

26

36

46

56

64

90

98

106

124

126

132

Table of Contents

Website

ALPCO continually strives to provide you with

informative and resourceful content instantly via our

website. The Clinical and Research sections allow you to

download tailored information in your area of interest,

or learn more about featured products and biomarkers

in our “Did You Know?” segments. For more detailed

information on a product you can visit the product pages

for specifications, protocols, or MSDS as well as check

pricing and order online 24/7. You’ll also want to visit for

updates on News, Events, and promotional information.

Resources

Our knowledgeable and responsive technical support

team is here to help you. View the Resources section of

our website for answers to frequently asked questions,

technical tips and interactive tools. You can submit

an inquiry at any time and our dedicated technical

representatives will assist in finding an optimal solution to

your testing needs.

Gastroenterology

4

Allergy & Infectious Disease

Products are for Research Use Only unless otherwise noted * FDA Registered for In Vitro Diagnostic Use ^ Health Canada Licensed

The immune system protects our body against several factors including allergens,

harmful pathogens and foreign particles. Allergies involve an exaggerated response of

the immune system and are among the most common chronic conditions worldwide.

Infectious disease agents are also one of the leading contributors to debilitating

and sometimes fatal conditions. Therefore, researchers and practitioners look to

understand interactions between microbes and the immune system in order to

translate this knowledge into treatment, control and prevention of conditions caused by

allergies and infectious disease.

Allergy Testing

Identification of allergens causing immediate type

reactions can be a very difficult task for allergists.

The method of investigation usually starts with patient

anamnesis followed by skin and specific IgE tests.

However, results from these tests are often unclear and

inconclusive due to cross reactivity, low sensitivity, or

non-IgE mediated allergies, making a provocation test

necessary, bearing the risk of an anaphylactic event.

5


FlowCAST® Basophil Activation Assays

ALPCO is the exclusive distributor of BÜHLMANN

Laboratories’ FlowCAST® allergy assays and allergens.

FlowCAST® offers an easy, fast and reliable solution

for allergy determination utilizing basophil activation

measurement via flow cytometry. In vitro basophil

activation testing reduces the risk of an anaphylactic

event and patient discomfort while also providing a

sensitive, quantitative measurement of non-IgE mediated

reactions which serum IgE methods are

unable to achieve.

Upon allergen contact, sensitized basophils degranulate

and express the cell surface marker CD63. In addition,

the surface marker CD203c is upregulated. By labeling

specific antibodies against these surface markers with

fluorochromes, their relative number can be measured

with any four parameter flow cytometer.

Selection of Basophils: Basophils constitutively express

the eotaxin receptor CCR3 allowing for a robust and

accurate selection of cells. CCR3 is measured via a PE-

fluorescence labeled monoclonal antibody. This gating of

basophil cells is significantly easier than with any other

commercially available basophil activation test.

Description Catalog # Range Sensitivity Sample Size Sample Type Incubation

Adenovirus IgA ELISA 20-AVAHU-E01Cut-off: See Protocol

See Protocol 10 µL Serum 1h 45m

Adenovirus IgG ELISA 20-AVGHU-E01Cut-off: See Protocol


Adenovirus IgM ELISA 20-AVMHU-E01Cut-off: See Protocol


Anti-Cag-A IgG ELISA 27-GD33Cut-off: See Protocol

6.25 U/mL 10 µL Plasma, Serum 1h 10m

Anti-Candida IgA ELISA 27-GD19 12.5–100 U/mL 0.5 U/mL 5 µL Serum 1h 10m

Anti-Candida IgG ELISA 27-GD22 12.5–100 U/mL 0.5 U/mL 5 µL Serum 1h 10m

Anti-Candida IgM ELISA 27-GD20Cut-off: See Protocol


Anti-Casein IgA ELISA 27-GD42Cut-off: See Protocol

See Protocol 10 µL Plasma, Serum 1h 10m

Anti-Chlamydia IgG ELISA 20-CGGHU-E01 See Protocol See Protocol 10 µL Serum 1h 45m

Anti-Cytomegalovirus IgG ELISA

27-GD84Cut-off: See Protocol

See Protocol 5 µL Plasma, Serum 50m

Gastroenterology

6



Activation of Basophils: CD63 and CD203c are

currently the best validated basophil activation

markers directly linked to basophil degranulation.

The FlowCAST® assay detects CD63 via a FITC-

fluorescence labeled monoclonal antibody, while the

FlowCAST® High Sensitivity assay detects both CD63

and CD203c via a tandem dye PE-647 fluorescence

labeled monoclonal antibody.

Stimulation Controls: The FlowCAST® assays provide

two stimulation controls, anti-FcεRI and fMLP. The

first control, anti-FcεRI, is a highly specific monoclonal

antibody binding to IgE receptor. The second control,

a tripeptide fMLP, activates basophils by a non-

immunological method.

Allergens: BÜHLMANN offers a portfolio of over 150

quality controlled and standardized allergens for use with

the basophil activation assays.


Anti-Cytomegalovirus IgM ELISA



Anti-Gliadin IgA ELISA 27-GD17 6.25–100 U/mL 0.25 U/mL 10 µL Serum 1h 10m

Anti-Gliadin IgA/IgG ELISA 27-GD77 6.25–100 U/mL 0.5 U/mL 10 µL Plasma, Serum 1h 10m

Anti-Gliadin IgG ELISA 27-GD16 6.25–100 U/mL 0.25 U/mL 10 µL Serum 1h 10m

Anti-HAV ELISA 22-E10 10–50 mIU/mL 0.1 IU/mL 10 µL Plasma, Serum 4h

Anti-HAV IgG ELISA 68-4AGE3Cut-off: See Protocol

See Protocol 10 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

1h 30m

Anti-HAV IgM ELISA 68-4AME3Cut-off: See Protocol

See Protocol 5 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

2h

Anti-HCV ELISA 68-4NAE3Cut-off: See Protocol


Anti-Helicobacter Pylori IgA ELISA



View basophil activation assays 01-FK-CCR and 01-FK-HSBAT on page 10

7



Anti-Helicobacter Pylori IgG ELISA

27-GD01 6.25–100 U/mL 6.25 U/mL 10 µL Plasma, Serum 1h 10m

Anti-Helicobacter Pylori IgM ELISA



Anti-Helicobacter Pylori Qualitative IgG ELISA



Anti-Helicobactor Pylori ELISA

30-6920Cut-off: See Protocol

See Protocol 100 mg Stool 3h

Anti-Herpes-1 IgG ELISA 27-GD86Cut-off: See Protocol


Anti-Herpes-1/2 IgM ELISA 27-GD88Cut-off: See Protocol


Anti-Herpes-2 IgG ELISA 27-GD87Cut-off: See Protocol


Anti-Ovalbumin IgA ELISA 27-GD40Cut-off: See Protocol


Anti-Ovalbumin IgG ELISA 27-GD41Cut-off: See Protocol


Anti-Ps-Aeruginosa IgA ELISA

27-GD18 6.25–100 U/mL 1.2 U/mL 100 µL Serum 1h 10m

Anti-Ps-Aeruginosa IgG ELISA


Anti-Rubella IgG ELISA 27-GD82Cut-off: See Protocol


Anti-Rubella IgM ELISA 27-GD83Cut-off: See Protocol


Anti-Soya IgA ELISA 27-GD44Cut-off: See Protocol


Aspergillus IgG Antibody ELISA


Bordetella Pertussis IgA ELISA

20-BPAHU-E01Cut-off: See Protocol


Bordetella Pertussis IgG ELISA

20-BPGHU-E01 * Cut-off: See Protocol


Bordetella Pertussis IgM ELISA

20-BPMHU-E01Cut-off: See Protocol


Brucella IgG ELISA 20-BRGHU-E01Cut-off: See Protocol


Brucella IgM ELISA 20-BRMHU-E01Cut-off: See Protocol


C. Trachomatis IFA 50-580-01 See Protocol See Protocol 10 µL Serum 1h

Casomorphin ELISA 30-7010 1–120 ng/mL 1 ng/mL 2 mL Urine Overnight

CAST® ELISA (Leukotriene Function)

01-EK-CAST 50–3,200 pg/mL 20 pg/mL 2 mL Whole Blood 2h 30m

Gastroenterology

8



West NileExposure to West Nile Virus (WNV) causes a disease with a number of symptoms including encephalitis. It is a potentially

neuroinvasive agent that causes asymptomatic infection and fever in humans. ALPCO’s FDA registered West Nile Virus IgM

ELISA employs a recombinant WNV antigen and can be used as a rapid serological marker for WNV infection.


CAST® ELISA Jumbo (Leukotriene Function)

01-EK-CAST5 50–3,200 pg/mL 20 pg/mL 2 mL Whole Blood 2h 30m

Chlamydia IgA IFA 50-513-01 See Protocol See Protocol 10 µL Serum 1h

Chlamydia IgG IFA 50-511-01 See Protocol See Protocol 10 µL Serum 1h

Chlamydia IgM IFA 50-512-01 See Protocol See Protocol 5 µL Serum 1h

Chlamydia Trachomatis IgA ELISA

20-CTAHU-E01 See Protocol See Protocol 10 µL Serum 1h 45m

Chlamydia Trachomatis IgM ELISA

20-CTMHU-E01 See Protocol See Protocol 10 µL Serum 1h 45m

DAO ELISA 30-8500 0–15.65 U/mL 0.052 U/mL 50 µL Serum 4h 20m

Dengue IgG ELISA 20-DEGHU-E01Cut-off: See Protocol


Dengue IgM ELISA 20-DEMHU-E01Cut-off: See Protocol


Dengue NS1 ELISA 65-DENHU-E01 See Protocol See Protocol 50 µL Serum 1h 51m

Echinococcus Granulosus IgG ELISA

20-EGGHU-E01 * Cut-off: See Protocol

See Protocol 10 µL Serum 20m

Epstein Barr Virus Nuclear Antigen IgG ELISA

20-ENGHU-E01Cut-off: See Protocol


Epstein Barr Virus Nuclear Antigen IgM ELISA

20-ENMHU-E01Cut-off: See Protocol


Epstein Barr Virus Viral Capsid Antigen IgA ELISA

20-EVAHU-E01Cut-off: See Protocol

See Protocol 10 µL Citrate Plasma, Serum 1h 45m

Epstein Barr Virus Viral Capsid Antigen IgG ELISA

20-EVGHU-E01Cut-off: See Protocol


Epstein Barr Virus Viral Capsid Antigen IgM ELISA

20-EVMHU-E01Cut-off: See Protocol


Fasciola Hepatica IgG ELISA 20-FHGHU-E01Cut-off: See Protocol


View west nile assays 65-WNGHU-E01 and 65-WNMHU-E01 on page 12

9



FlowCAST Highsens® High Sensitivity Basophil

Activation Test01-FK-HSBAT ^ See Protocol See Protocol 100 µL Whole Blood 1h

FlowCAST® Basophil Activation Test

01-FK-CCR See Protocol See Protocol 50 µL Whole Blood 30m

Herpes Simplex Virus 1 IgG ELISA

20-HSGHU-E01Cut-off: See Protocol


Herpes Simplex Virus 1 IgM ELISA

20-HSMHU-E01Cut-off: See Protocol


Herpes Simplex Virus 1/2 IgG ELISA

20-HG2HU-E01Cut-off: See Protocol


Herpes Simplex Virus 1/2 IgM ELISA

20-HM2HU-E01Cut-off: See Protocol


Herpes Simplex Virus 2 IgG ELISA

20-H2GHU-E01Cut-off: See Protocol



20-H2MHU-E01Cut-off: See Protocol


Histamine ELISA 17-HISHU-E01 0.3–30 ng/mL See Protocol 25 µL Plasma, Urine Overnight

Histamine ELISA 32-5890 0.39–50 nM 0.05 nM 200 µLBiological Fluids, Cell Culture, Plasma, Urine

Overnight

Histamine ELISA (Cell Culture)

17-HISCC-E01 0.5–50 ng/mL 0.2 ng/mL 20 µL Cell Culture, Urine 1h

Histamine ELISA (In Food) 17-HISHU-E01-FOOD See Protocol 0.15 µg/L See Protocol Food 2h

Histamine ELISA (Stool) 17-HISHU-E01-STL 0.5–50 ng/mL 6.1 ng/g See Protocol Stool 2h

Histamine Research ELISA 17-HISRT-E01-RES 0.3–30 ng/mL 0.2 ng/mL 10 µL EDTA Plasma Overnight

Influenza A IgA ELISA 20-IAAHU-E01Cut-off: See Protocol


Influenza A IgG ELISA 20-IAGHU-E01Cut-off: See Protocol


Influenza A IgM ELISA 20-IAMHU-E01Cut-off: See Protocol


Influenza B IgA ELISA 20-IBAHU-E01Cut-off: See Protocol


Influenza B IgG ELISA 20-IBGHU-E01Cut-off: See Protocol


Influenza B IgM ELISA 20-IBMHU-E01Cut-off: See Protocol


Japanese Encephalitis IgG ELISA

65-JEGHU-E01 See Protocol See Protocol 4 µL Serum 2h 16m

Japanese Encephalitis IgM ELISA

65-JEMHU-E01 See Protocol See Protocol 4 µL Serum 3h 16m

Measles IgM ELISA 20-MEMHU-E01Cut-off: See Protocol


Gastroenterology

10




Mumps IgG ELISA 20-MUGHU-E01Cut-off: See Protocol


Mumps IgM ELISA 20-MUMHU-E01Cut-off: See Protocol


Mycobacterium Tuberculosis IgG ELISA

65-TBGHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

Mycobacterium Tuberculosis IgM ELISA

65-TBMHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

Mycoplasma Hominis IgA ELISA

20-MHAHU-E01Cut-off: See Protocol


Mycoplasma Hominis IgG ELISA

20-MHGHU-E01Cut-off: See Protocol


Mycoplasma Hominis IgM ELISA

20-MHMHU-E01Cut-off: See Protocol


Orientia Tsutsugamushi IgG ELISA

65-OTGHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

Orientia Tsutsugamushi IgM ELISA

65-OTMHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

Parvovirus B 19 IgG ELISA 20-PBGHU-E01Cut-off: See Protocol


Parvovirus B 19 IgM ELISA 20-PBMHU-E01Cut-off: See Protocol


Respiratory Syncytial Virus IgA ELISA

20-RSAHU-E01Cut-off: See Protocol


Respiratory Syncytial Virus IgG ELISA

20-RSVHU-E01Cut-off: See Protocol

See Protocol 10 µL Serum 2h

Respiratory Syncytial Virus IgM ELISA

20-RSMHU-E01Cut-off: See Protocol


Syphilis IgG ELISA 20-SYGHU-E01Cut-off: See Protocol


Syphilis IgM ELISA 20-SYMHU-E01Cut-off: See Protocol


Syphilis Screen ELISA 20-SYSHU-E01Cut-off: See Protocol


Syphilis Screen IgG/IgM ELISA

20-TPSHU-E01Cut-off: See Protocol


Tetanus Toxin IgG ELISA 20-TETHU-E01Cut-off: See Protocol


Toxoplasma Gondii IgA ELISA

20-TGAHU-E01Cut-off: See Protocol


Toxoplasma Gondii IgM ELISA

20-TGMHU-E01Cut-off: See Protocol


ToxoPlasma IgG Ab ELISA 27-GD80Cut-off: See Protocol

See Protocol 5 µL Plasma, Serum See Protocol

11



Ureaplasma Urealyticum IgA ELISA

20-UUAHU-E01Cut-off: See Protocol


Ureaplasma Urealyticum IgG ELISA

20-UUGHU-E01Cut-off: See Protocol


Ureaplasma Urealyticum IgM ELISA

20-UUMHU-E01Cut-off: See Protocol


Varicella Zoster IgA ELISA 20-VZAHU-E01Cut-off: See Protocol


Varicella Zoster IgG ELISA 20-VZGHU-E01Cut-off: See Protocol


Varicella Zoster IgM ELISA 20-VZMHU-E01Cut-off: See Protocol


Visceral Leishmaniasis ELISA 65-VILHU-E01 See Protocol See Protocol 10 µL Serum 1h 16m

West Nile Virus IgG ELISA 65-WNGHU-E01 See Protocol See Protocol 4 µL Serum 2h 15m

West Nile Virus IgM ELISA 65-WNMHU-E01 * See Protocol See Protocol 4 µL Serum 3h 16m

Gastroenterology

12



Allergen Category Sub-Category Description Catalog Number

DRUGS Adjuvants Carboxymethylcellulose 05-BAG2-CE466

DRUGS Adjuvants PEG-4000 05-ABG2-CPEG

DRUGS Analgesic Acetaminophen 05-BAG2-C55

DRUGS Analgesic Diclofenac 05-BAG2-C52

DRUGS Analgesic Dipyrone/Metamizole 05-BAG2-C59

DRUGS Analgesic Ibuprofen 05-BAG2-C53

DRUGS Analgesic Indomethacin 05-BAG2-C54

DRUGS Analgesic Lys-Aspirin 05-BAG2-C51

DRUGS Analgesic Mefenamic Acid 05-BAG2-C56

DRUGS Analgesic Naproxen 05-BAG2-CNAP

DRUGS Analgesic Phenylbutazone 05-BAG2-C57

DRUGS Analgesic Propyphenazone 05-BAG2-C58

DRUGS Antibiotic Amoxicillin 05-BAG2-C204

DRUGS Antibiotic Ampicillin 05-BAG2-C203

DRUGS Antibiotic Benzylpenicilloyl + - Acid, MDM 05-BAG2-C12

DRUGS Antibiotic Benzylpenicilloyl -Polylysin, PPL 05-BAG2-C11

DRUGS Antibiotic Cefaclor 05-BAG2-C34

DRUGS Antibiotic Cefamandole 05-BAG2-C31

DRUGS Antibiotic Cefazolin 05-BAG2-C32

DRUGS Antibiotic Cefuroxime 05-BAG2-C33

DRUGS Antibiotic Cephalosporin C 05-BAG2-C3

DRUGS Antibiotic Cetriaxone 05-BAG2-C35

DRUGS Antibiotic Ciprofloxacin 05-BAG2-C81

DRUGS Antibiotic Clarythromycin 05-BAG2-CCLA

DRUGS Antibiotic Clavulanic Acid 05-BAG2-CCLAV

DRUGS Antibiotic Levofloxacin 05-BAG2-C36

DRUGS Antibiotic Penicillin G 05-BAG2-C1

DRUGS Antibiotic Penicillin V 05-BAG2-C2

DRUGS Antibiotic Rifampicin 05-BAG2-CRIF

13

Products are for Research Use Only unless otherwise noted * FDA Registered for In Vitro Diagnostic Use All Allergens are Health Canada Licensed


DRUGS Antibiotic Sulfamethoxazole 05-BAG2-C61

DRUGS Antibiotic Tetracycline 05-BAG2-C75

DRUGS Antibiotic Trimethoprim 05-BAG2-C62

DRUGS Antiseptic Chlorhexidine 05-BAG2-CCHX

DRUGS Contrast Media Iobitridol 05-BAG2-CIOBI

DRUGS Contrast Media Iodixanol 05-BAG2-CIODI

DRUGS Contrast Media Iohexol 05-BAG2-CIOHE

DRUGS Contrast Media Iomeprol 05-BAG2-CIOME

DRUGS Contrast Media Iopamidol 05-BAG2-CIOPA

DRUGS Contrast Media Iopromide 05-BAG2-CIOPR

DRUGS Contrast Media Ioxaglate 05-BAG2-CIOXA

DRUGS Contrast Media Ioxitalamate 05-BAG2-CIOXI

DRUGS Local Anesthetic Articaine 05-BAG2-CART

DRUGS Local Anesthetic Bupivacaine 05-BAG2-CBUP

DRUGS Local Anesthetic Lidocaine 05-BAG2-CLID

DRUGS Local Anesthetic Mepivacaine 05-BAG2-CMEP

DRUGS Myorelaxant Atracurium 05-BAG2-CATR

DRUGS Myorelaxant Cisatracurium 05-BAG2-CCAT

DRUGS Myorelaxant Mivacurium 05-BAG2-CMIV

DRUGS Myorelaxant Pancuronium 05-BAG2-CPAN

DRUGS Myorelaxant Propofol 05-BAG2-CPRO

DRUGS Myorelaxant Rocuronium 05-BAG2-CROC

DRUGS Myorelaxant Suxamethonium 05-BAG2-CSUX

DRUGS Myorelaxant Vecuronium 05-BAG2-CVEC

DRUGS Proton Pump Inhibitors Esomeprazole 05-BAG2-CESO

DRUGS Proton Pump Inhibitors Lansoprazole 05-BAG2-CLAN

DRUGS Proton Pump Inhibitors Omeprazole 05-BAG2-COMe

DRUGS Proton Pump Inhibitors Pantoprazole 05-BAG2-CPANT

DRUGS Proton Pump Inhibitors Rabeprazole 05-BAG2-CRAB

Gastroenterology

14




ENVIRONMENTAL/OCCUPATIONAL Environmental/Occupational Chloramine-T 05-BAG-K85

ENVIRONMENTAL/OCCUPATIONAL Environmental/Occupational Latex 05-BAG-K82

ENVIRONMENTAL/OCCUPATIONAL Environmental/Occupational Phtalic Anhydride/Acid 05-BAG-K79

ENVIRONMENTAL/OCCUPATIONAL Environmental/Occupational α-Amylase 05-BAG-K87

FOOD Egg Egg White 05-BAG-F1

FOOD Egg Egg Yolk 05-BAG-F75

FOOD Fish/Meat Anisakis 05-BAG-P4

FOOD Fish/Meat Beef 05-BAG-F27

FOOD Fish/Meat Codfish 05-BAG-F3

FOOD Fish/Meat Crab 05-BAG-F23

FOOD Fish/Meat Oyster 05-BAG-F290

FOOD Fish/Meat Pork 05-BAG-F26

FOOD Fish/Meat Shrimp 05-BAG-F24

FOOD Fish/Meat Squid 05-BAG-F258

FOOD Fruit/Vegetable Carrot 05-BAG-F31

FOOD Fruit/Vegetable Celery 05-BAG-F85

FOOD Fruit/Vegetable Orange 05-BAG-F33

FOOD Fruit/Vegetable Peach 05-BAG-F95

FOOD Fruit/Vegetable Tomato 05-BAG-F25

FOOD Grain Baker's Yeast 05-BAG-F45

FOOD Grain Barley Flour 05-BAG-F6

FOOD Grain Maize 05-BAG-F8

FOOD Grain Maize Starch 05-BAG-F81

FOOD Grain Oat Flour 05-BAG-F7

FOOD Grain Rice 05-BAG-F9

FOOD Grain Rye Flour 05-BAG-F5

FOOD Grain Wheat 05-BAG-F4

FOOD Milk/Milk Product Casein 05-BAG-F78

FOOD Milk/Milk Product Cow Milk 05-BAG-F2

15



FOOD Milk/Milk Product α-Lactalbumin 05-BAG-F76

FOOD Milk/Milk Product β-Lactoglobulin 05-BAG-F77

FOOD Seed/Bean/Nut Almond 05-BAG-F20

FOOD Seed/Bean/Nut Cashew Nut 05-BAG-F202

FOOD Seed/Bean/Nut Hazelnut 05-BAG-F17

FOOD Seed/Bean/Nut Peanut 05-BAG-F13

FOOD Seed/Bean/Nut Peanut nARA H 1 05-BAG2-ARAH1



FOOD Seed/Bean/Nut Sesame 05-BAG-F10

FOOD Seed/Bean/Nut Soybean 05-BAG-F14

FOOD ADDITIVES Food Additive Amaranth 05-BAG2-CE123

FOOD ADDITIVES Food Additive Brilliant Black BN 05-BAG2-CE151

FOOD ADDITIVES Food Additive Chromotrope B 05-BAG2-CE122

FOOD ADDITIVES Food Additive Erythosine 05-BAG2-CE127

FOOD ADDITIVES Food Additive Glutamate 05-BAG2-CE621

FOOD ADDITIVES Food Additive Indigo Carmine 05-BAG2-CE132

FOOD ADDITIVES Food Additive Methylene Blue 05-BAG2-C105

FOOD ADDITIVES Food Additive New Coccine 05-BAG2-CE124

FOOD ADDITIVES Food Additive Patent Blue V 05-BAG2-CE131

FOOD ADDITIVES Food Additive Potassium-Metabisulfite 05-BAG2-C113

FOOD ADDITIVES Food Additive Quinoline Yellow 05-BAG2-CE104

FOOD ADDITIVES Food Additive Salicylic Acid 05-BAG2-C114

FOOD ADDITIVES Food Additive Sodium-Benzoate 05-BAG2-C111

FOOD ADDITIVES Food Additive Sodium-Nitrite 05-BAG2-C112

FOOD ADDITIVES Food Additive Sorbic Acid 05-BAG2-CE202

FOOD ADDITIVES Food Additive Sunset Yellow FCF 05-BAG2-CE110

FOOD ADDITIVES Food Additive Tartrazine 05-BAG2-C103

INHALANTS Grass Bermuda Grass 05-BAG-G2

Gastroenterology

16




INHALANTS Grass Cultivated Rye Grass 05-BAG-G12

INHALANTS Grass Orchard Grass 05-BAG-G3

INHALANTS Grass Perennial Rye Grass 05-BAG-G5

INHALANTS Grass Timothy Grass 05-BAG-G6

INHALANTS Mite/Animal/Insect Acarus siro 05-BAG-D70

INHALANTS Mite/Animal/Insect Cat Epithelium 05-BAG-E1

INHALANTS Mite/Animal/Insect D. farinae 05-BAG-D2

INHALANTS Mite/Animal/Insect D. pteronyssinus 05-BAG-D1

INHALANTS Mite/Animal/Insect Dog Epithelium 05-BAG-E2

INHALANTS Mold Alternaria tenuis 05-BAG-M6

INHALANTS Mold Aspergillus fumigatus 05-BAG-M3

INHALANTS Mold Candida albicans 05-BAG-M5

INHALANTS Mold Cladosporium herbarum 05-BAG-M2

INHALANTS Mold Penicillium notatum 05-BAG-M1

INHALANTS Tree Common Birch 05-BAG-T3

INHALANTS Tree Hazel 05-BAG-T4

INHALANTS Tree Oak 05-BAG-T7

INHALANTS Tree Olive 05-BAG-T9

INHALANTS Weed Common Ragweed 05-BAG-W1

INHALANTS Weed Mugwort 05-BAG-W6

INHALANTS Weed Parietaria Officinalis 05-BAG-W19

INHALANTS Weed Ribwort/Plantain 05-BAG-W9

INSECT VENOMSCrossreactive Carbohydrate Determinants

Bromelain 05-BAG-K202

INSECT VENOMSCrossreactive Carbohydrate Determinants

HRP 05-BAG-K225

INSECT VENOMS Insect Venom European Hornet 05-BAG2-I75

INSECT VENOMS Insect Venom Honey Bee Venom 05-BAG2-I1

INSECT VENOMS Insect Venom Honey Bee Venom Check 05-BAG2-I1CHK

INSECT VENOMS Insect Venom Paper Wasp Venom 05-BAG2-I4

17



INSECT VENOMS Insect Venom Yellow Jacket Venom 05-BAG2-I3

INSECT VENOMS Insect Venom Yellow Jacket Venom Check 05-BAG2-I3CHK

RECOMBINANT ALLERGENS Recombinant Allergen FEL D 1 (Cat Major Allergen) 05-BAG2-FELD1

RECOMBINANT ALLERGENS Recombinant Allergen MAL D 1 (Apple Major Allergen) 05-BAG2-MALD1

RECOMBINANT ALLERGENS Recombinant Allergen TRI A 12 (Wheat Profilin) 05-BAG2-TRIA12

RECOMBINANT ALLERGENS Recombinant Allergen ZEA M 12 (Maize Profilin) 05-BAG2-ZEAM12

ALLERGEN MIXES Allergen Mix6 Grass Mix (Orchard, Meadow fescue, Perenial rye, Timothy, Meadow, Velvet grass)

05-BAG-GX1

ALLERGEN MIXES Allergen MixFood Colorants I Mix (Quinoline yellow, Sunset yellow FCF, Chromotrope B, Amaranth, New coccine)

05-BAG-C101

ALLERGEN MIXES Allergen MixFood Colorants II Mix (Erythrosine, Patent blue V, Indigo carmine, Brilliant black BN)

05-BAG-C102

ALLERGEN MIXES Allergen MixFood Mix (Egg white, Egg yolk, Milk, Codfish, Wheat, Peanut, Soybean, Hazelnut, Shrimp)

05-BAG-FX1

ALLERGEN MIXES Allergen Mix

Inhalant Mix (6-grass mix, Cultivated rye grass, Common birch, Hazel, Mugwort, Ribwort/Plantain, Alternaria tenuis, D. pteronissinus, D. farinae, Cat epithelium, Dog epithelium)

05-BAG-IX1

ALLERGEN MIXES Allergen MixNeuromuscular Blockers Mix (Atracurium, Cisatracurium, Mivacurium, Pancurium, Propofol, Rocuronium, Suxamethonium, Vecuronium)

05-BAG2-CNBX

ALLERGEN MIXES Allergen Mix Ragweed Mix (Common and Giant ragweed) 05-BAG-WX1

ALLERGEN MIXES Allergen MixStorage Mite Mix (Acarus Siro, Lepidoglyphus destructor, Tryophagus putrescentia, Glycyphagus domesticus)

05-BAG-DX1

Gastroenterology

18



Bone Metabolism

20


Bone Metabolism

Bone metabolism is a continual, cyclic interplay of bone growth and resorption that is

carefully orchestrated by the dynamic relationship between osteoclasts, osteoblasts

and an array of hormonal and regulatory influences. The relative levels of these

signaling molecules dictate whether healthy, balanced bone metabolism ensues.

Disturbances to this delicate equilibrium, where reabsorption is greater than growth,

can weaken the skeletal architecture and put one at risk for the development of chronic

and debilitating diseases such as osteoporosis.

As we continue to gain a better understanding of the intricacies of bone metabolism and the key regulators involved,

we may also gain further insights into the underlying mechanisms of bone-related diseases such as:

• Osteopetrosis

• Paget’s Disease

• Rheumatoid Arthritis

• Cancer & Bone Metastases

• Immobilization-induced

bone loss

• Chronic Kidney Disease

21


1,25 (OH)2 Vitamin D ELISA

30-2112 5.1–200 pg/mL 4.8 pg/mL 500 µL Plasma, Serum Overnight

1,25 (OH)2 Vitamin D Immunotube Extraction Kit

30-KM1100 See Protocol See Protocol 500 µL Plasma, Serum 1h 8m

25-OH Vitamin D ELISA 30-2109 2–300 nmol/L 2 nmol/L 30 µL Serum Overnight

25-OH Vitamin D HPLC 30-3400 4–1,250 nmol/L 4 nmol/L 500 µL Plasma, Serum See Protocol

Anti-TNF α Inhibitor ELISA (Antibody

Against ENBREL®)30-9653

Cut-off: See Protocol

See Protocol 100 µL Serum Overnight

Anti-TNF α Inhibitor ELISA (Antibody Against Humira®)


See Protocol 100 µL EDTA Plasma, Serum Overnight

Bone Sialoprotein ELISA 30-4222 3.1–50 ng/mL 1 ng/mL 100 µL Serum 3h

Calcitonin ELISA 21-CALHU-E01 * 10–1,000 pg/mL 1 pg/mL 100 µL Serum 4h 30m

Calcitonin Mouse/Rat IRMA 31-CALRT-R100 12–1,800 pg/mL 0.6 pg/mL 200 µLCell Culture, Plasma, Serum

Overnight

Cathepsin K ELISA 04-BI-20432 11–300 pmol/L 1.1 pmol/L 50 µL Cell Culture, Serum Overnight

SclerostinSclerostin, a protein product of the SOST gene, inhibits

osteoblast activity via antagonism of the Wnt signaling

pathway and plays a key role in the regulation of bone

formation. Reports show that sclerostin expression

and/or circulating levels are elevated in osteoporosis,

immobilization-induced bone loss, rheumatoid

arthritis, multiple myeloma and bone metastases,

making it a therapeutic target of great interest for

the fields of bone and cancer research.1,2,3 There are

indications that sclerostin levels are also elevated with

chronic kidney disease, suggesting relevance in the

study of renal insufficiency. 4,5

Mouse Sclerostin ELISA ALPCO has developed a novel mouse sclerostin

immunoassay and confirmed its specificity through

validation via testing in a sclerostin knock out mouse.

This assay is a highly sensitive ELISA utilizing a polyclonal

antibody based sandwich format for detecting low

circulating levels of sclerostin in mouse samples. It

requires a small volume of serum or heparin plasma, which

facilitates time-course sampling and/or the measurement

of multiple biomarkers with one collection.

Bone Metabolism

22



CTGF ELISA 20-CTGHU-E01 1–500 ng/mL 3.24 ng/mL 25 µL Serum 3h 45m

DKK-1 ELISA 04-BI-20412 3.125–50 pmol/L 0.38 pmol/L 15 µL Serum Overnight

DPD ELISA (Urine) 55-DE8007 * 3–300 nmol/L 1.1 nmol/L 50 µL Urine 3h

Fetuin-A ELISA 43-FETHU-E01 * 0.5–3.7 ng/mL 5 ng/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h

Fetuin-A Ultrasensitive ELISA

43-FETHUU-E01 * 0.01–1.30 g/L 40 ng/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture

2h 45m

hCOMP ELISA 13-COMP-200 10–80 ng/mL 10 ng/mL 5 µL Serum Overnight

Hydroxy Pyridinium Crosslinks HPLC

Assay (PYD/DPD)30-3201

7.3–2,500 pmol/mL

7.3 pmol/mL 500 µL Urine See Protocol

IL-6 ELISA 45-IL6HU-E01 7.8–500 pg/mL 2 pg/mL 100 µLCell Culture, EDTA Plasma, Serum

3h


3h

Human Sclerostin ELISA The Human Sclerostin ELISA is a highly referenced kit as

a regulatory marker of bone turnover, requiring only a 20

µL sample of human plasma or serum. The measurement

of circulating sclerostin in conjunction with other

biomarkers of bone turnover is becoming increasingly

common in the study of bone disorders.

1 Rey JP, Ellies DL. Wnt modulators in the biotech pipeline. Developmental

Dynamics. Mar 2010; Vol 239 No 3: 102-114.

2 Gaudio et al. Increased sclerostin serum levels associated with bone

formation and resorption markers in patients with immobilization-induced

bone loss. J Clin Endocrinol Metabolism. May 2010; Vol 95 No 5: 2248-

2253.

3 Colucci S et al. Myeloma cells suppress osteoblasts through sclerostin

secretion. Blood Cancer. Jun 2011; Vol 1 No 6: e27.

4 Drüeke TB & Lafage-Proust. Sclerostin: just one more player in renal bone

disease? Clin J Am Soc Nephrology. Apr 2011; Vol 6, No 4: 700-703.

5 Cejka D et al. Sclerostin and Dickkopf-1 in renal osteodystrophy. Clin J Am

Soc Nephrology. Apr 2011; Vol 6 No 4: 877-882.

23


View sclerostin assays 04-BI-20492 on page 24, and 41-SCLMS-E01 on page 25


IL-6 Monkey ELISA 61-IL6MK-E01 1.9–120 U/mL 1.3 U/mL 50 µL Cell Culture, Serum 3h 10m

IL-6 Mouse ELISA 61-IL6MS-E0131.3–2,000 pg/mL

6.5 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Serum

3h 30m

IL-6 Rat ELISA 61-IL6RT-E01 31–2,000 pg/mL 12 pg/mL 50 µL Cell Culture, Serum 3h 10m

Intact PTH Bovine ELISA 31-IPTBO-E01 0–10,000 pg/mL 10 pg/mL 50 µLCell Culture, Plasma, Serum

3h 30m

Myostatin ELISA 30-1012 0.27–195 ng/mL 0.27 ng/mL 30 µL Plasma, Serum 5h

Nt-proCNP ELISA 04-BI-20872 2–40 pmol/L 0.55 pmol/L 50 µLCell Culture, Plasma, Serum

4h 30m

Osteocalcin ELISA (1-43/49) 43-OSNHU-E01 0–64 ng/mL 0.31 ng/mL 25 µL Serum 1h 20m

Osteocalcin Intact ELISA 38-OSTHU-E01 2–90 ng/mL 0.4 ng/mL 25 µL Serum 2h 30m

Osteocalcin Mouse ELISA 31-OSTMS-E01 3.2–95 ng/mL 0.4 ng/mL 20 µLCell Culture, Plasma, Serum

3h

Osteocalcin Rat ELISA 31-OSTRT-E01 0.3–35 ng/mL 0.05 ng/mL 20 µLCell Culture, Plasma, Serum

3h

Osteocalcin RIA 72-OSTEO-CT-US 0–300 ng/mL 0.4 ng/mL 50 µLEDTA Plasma, Heparin Plasma, Plasma, Serum

2h

Osteopontin ELISA 41-OPNHU-E01 0.31–20 ng/mL 0.05 ng/mL 10 µL Heparin Plasma, Urine 3h

Osteopontin Mouse ELISA 41-OPNMS-E01 0.03–2.0 ng/mL 0.015 ng/mL 5 µL Plasma 3h

Osteoprotegerin ELISA 04-BI-20403 25–400 pg/mL 1.4 pg/mL 20 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Serum

5h 30m

Osteoprotegerin Mouse/Rat ELISA

30-1020125–4,000 pg/mL

125 pg/mL 100 µLCell Culture, Plasma, Serum, Urine

Overnight

Procollagen III Peptide RIA 72-OCFK07-PIIIP 0.1–14 U/mL 0.1 U/mL 20 µL Plasma, Serum 5h

PTH Bioactive Intact Rat ELISA

31-BIPRT-E01 25–2,700 pg/mL 3 pg/mL 25 µL Cell Culture, Plasma 3h 30m

PTH High Sensitive ELISA (1-34)

31-IPTHUU-E01 3.2–143 pg/mL 0.3 pg/mL 150 µLCell Culture, EDTA Plasma, Plasma

3h 30m

PTH Intact ELISA (1-84) 21-IPTHU-E01 * 7–700 pg/mL 1.57 pg/mL 25 µL Serum 3h 30m

PTH Intact Mouse ELISA (1-84)

31-PTHMS-E0132–1,045 pg/mL or 336–2,616 pg/mL

4 pg/mL 20 µL Cell Culture, Plasma 3h 30m

PTH Intact Porcine ELISA 31-IPTPO-E01 0–1,000 pg/mL 1 pg/mL 50 µLCell Culture, Plasma, Serum

3h 30m

PTH Intact Rat ELISA (1-84) 31-IPTRT-E01 25–2,700 pg/mL 1.6 pg/mL 25 µLCell Culture, Plasma, Serum

3h 30m

Sclerostin ELISA 04-BI-20492 15–240 pM 2.6 pM 20 µLEDTA Plasma, Heparin Plasma, Serum

Overnight

Bone Metabolism

24



Sclerostin Mouse ELISA 41-SCLMS-E0137.5–1,200 pg/mL

17.4 pg/mL 15 µL Heparin Plasma, Serum 5h 20m

sRANKL Mouse/Rat ELISA 30-1019125–4,000 pg/mL

125 pg/mL 100 µLCell Culture, Serum, Urine

Overnight

sRANKL Total ELISA 30-1016 0–60 pg/mL 1.5 pg/mL 10 µLCell Culture, Plasma, Serum

Overnight

Vitamin D Binding Protein ELISA

30-2314 2.2–60 ng/mL 1.23 ng/mL 10 µL Plasma, Serum, Urine 2h 20m

Vitamin D Binding Protein Rat ELISA

41-VDBRT-E013.125–100 ng/mL

3.125 ng/mL 5 µL Plasma, Serum 1h 30m

Vitamin D2/D3 HPLC 30-3420 4–1,250 nmol/L 4 nmol/L 500 µL Plasma, Serum See Protocol

Vitamin K1 HPLC 30-2400 See Protocol 0.15 ng/mL 1 mL Plasma, Serum See Protocol

25


26



Cardiovascular Disease (CVD) encompasses a constellation of diseases related to the

heart and/or circulatory system. Despite efforts to understand and combat heart-

related disease, the first and fourth leading causes of death in the United States

are coronary disease and ischemic stroke, respectively.1 This is striking, considering

extensive campaigns to educate the public on diet and lifestyle factors that are, in

many cases, controllable. Two primary risk factors for CVD include obesity and Type

2 Diabetes, both of which have continued to be on the rise over the past few decades.

It is evident that regardless of the advancements in scientific knowledge of the disease

and the improvements in diagnosis and treatment, more research is necessary to

further our understanding of CVD.

The American Heart Association has dedicated $3.5 billion to the research of CVD and stroke since the late 1940’s,1

and it continues to fund a spectrum of research from community outreach to genomics. These efforts, combined

with federal and philanthropic funding, facilitate the research required for the identification of novel biomarkers and

testing their relevance in basic, preclinical and clinical research.

1 American Heart Association – Research. 2013. <http://my.americanheart.org/professional/Research/Research_UCM_316889_SubHomePage.jsp>


27


11,12-DHET ELISA 60-DH4 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

See Protocol

11,12-DHET ELISA (10 kits) 60-DH104 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

See Protocol


See Protocol


See Protocol

14,15-DHET ELISA 60-DH1 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

See Protocol


See Protocol


See Protocol


See Protocol

20-HETE ELISA 60-20H1 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

See Protocol

20-HETE ELISA (2 kits) 60-20H11 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

See Protocol

20-HETE ELISA (5 kits) 60-20H21 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

See Protocol

Angiotensin Converting EnzymeAngiotensin Converting Enzyme (ACE) catalyzes the conversion of angiotensin I to angiotensin II and inactivates

bradykinin during blood pressure regulation via the renin-angiotensin system. Measurement of ACE levels with ALPCO’s

FDA registered assay has been shown to aid in the diagnosis and monitoring of conditions such as sarcoidosis, diabetic

nephropathy and cardiovascular disease.1

1 Steven A. Atlas, MD. The Renin-Angiotensin Aldosterone System: Pathophysiological Role and Pharmacologic Inhibition. JMCP. Oct 2007; Vol 13 No 8.

View ACE assays 01-KK-ACK and 01-KK-ACF on page 2928




8-isoPGF2a ELISA 60-8ISO1 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

See Protocol

8-isoPGF2a ELISA (10 kits) 60-8ISO101 See Protocol 10 pg/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

Overnight


See Protocol


See Protocol

ACE Kinetic 01-KK-ACK * ^ 3–150 ACE units 5 ACE Units 25 µL Serum 15m

ACE Kinetic 01-KK-ACK2 * ^ 3–150 ACE Units 5 ACE Units 25 µL Serum 15m

ACE Kinetic High Sensitive 01-KK-ACF 1–30 U/L 1 U/L 80 µL Cerebrospinal Fluid See Protocol

ACE Kinetic Jumbo (1200) 01-KK-ACKX * ^ 3–150 ACE units 5 ACE Units 25 µL Serum 15m

AcSDKP ELISA (N-acetyl-Ser-Asp-Lys-Pro)

32-5881 0.19–25 nM 0.19 nM 150 µL Plasma, Serum, Urine Overnight

ACTH ELISA 21-ACTHU-E01 * 5–500 pg/mL 0.46 pg/mL 200 µL Plasma 5h

Adenosine HPLC 30-8000 0.78–6.84 µM 0.78 µM 500 µL Urine 1h 30m

ADMA ELISA 30-7828 0.1–2 µmol/L 0.05 µmol/L 50 µLCitrate Plasma, EDTA Plasma, Plasma, Serum

Overnight

ADMA Mouse/Rat ELISA 30-3001 0.1–3 µmol/L 0.05 µmol/L 100 µL EDTA Plasma, Serum Overnight

ANP (Atrial Natriuretic Peptide)

is released into circulation from

atrial myocytes in response to fiber

contraction and elevated levels can be

predictive of cardiac dysfunction.1,2

BNP (Brain-derived Natriuretic

Peptide) is expressed in the

ventricular myocardium and

released with contraction. Elevated

levels of BNP can be predictive of

heart failure.1,2

ALPCO offers several assays for the

direct measurement of natriuretic

peptides using small sample volumes

which do not require extraction.

1 Hoffmann U et al. Increased plasma levels of NT-proANP and NT-proBNP as markers of cardiac dysfunction in septic patients. Clin Lab. 2005; Vol 51: 373-379.

2 Ellinor PT, et al. Discordant atrial natriuretic peptide and brain natriuretic peptide levels in lone atrial fibrillation. J Am Coll Cardiology. Jan 2005; Vol 45 No 1:

82-86.

View ANP assays 32-5103, 13-BANI 1 and BNP assay 04-BI-20852W on page 30 29



ADMA Xpress ELISA 30-7860 0.1 µM–2 µM 0.1 µM 25 µL EDTA Plasma, Serum 3h

Angiotensin II ELISA 32-5880 1–125 pg/mL 1 pg/mL 100 µL Plasma Overnight

Angiotensin II RIA 01-RK-A22 * 2–500 pg/mL 1 pg/mL 1 mL Plasma Overnight

ANP Rat ELISA 32-510315.6–2,000 pg/mL

15.6 pg/mL 50 µLCitrate Plasma, EDTA Plasma

Overnight

ANP RIA 13-BANI 1 7.8–1,000 pg/mL 3.5 pg/mL 500 µL Plasma Overnight

Anti-GBM & ANCA Rapid Screen ELISA

13-GCP-100 * See ProtocolCut-off: See Protocol

25 µL Serum 30m

Antioxidative Capacity ELISA

30-5200 130–393 µmol/L 130 µmol/L 20 µL Plasma, Serum 15m

Antithrombin III Rat ELISA 41-AT3RT-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Serum 1h 40m

AOPP Colorimetric Assay 30-7811w 6.25–100 µmol/L 1.5 µmol/L 40 µL EDTA Plasma, Plasma 30m

AOPP Colorimetric Assay (Cuvette)

30-7811c 6.25–100 µmol/L 1.1 µmol/L 400 µL EDTA Plasma, Plasma 30m

Apolipoprotein A1 ELISA 41-APOHU-E01 15.6–500 ng/mL 15.6 ng/mL 100 µL Plasma, Serum 1h 40m

BNP Fragment ELISA (Nt-proBNP 8-29)

04-BI-20852W 5–1,000 fmol/mL 5 fmol/mL 35 µLCell Culture, Plasma, Serum

Overnight

Bradykinin ELISA 48-BRAHU-E017.2–5,000 pg/well

7.2 pg/mL 10 µL Plasma, Urine Overnight

ADMA (Asymmetric

Dimethylarginine) is a protein

found in blood plasma where

elevated levels inhibit synthesis

of nitric oxide thus resulting in

vasoconstriction and increased

blood pressure.1,2 The ADMA

Xpress ELISA allows for quantitative

measurement in human samples

with results in less than four hours.

1 Bouras G et al. Asymmetric Dimethylarginine (ADMA): a promising biomarker for cardiovascular

disease? Curr Top Med Chem. 2013; Vol 13 No 2: 180-200.

2 Böger RH et al. Asymmetric dimethylarginine (ADMA): a novel risk factor for endothelial dysfunction:

its role in hypercholesterolemia. Circulation. Nov 1998; Vol 98 No 18:1842-1847.

L-Arginine L-Citrullin + NO

has vasodilatory effects

inhibits monocyte adhesion

inhibits smooth musclecell proliferation

inhibits platelet aggregation

NO=Endogenous anti-arteriosclerotic molecule:

NO-Synthase

ADMAinhibits

View the ADMA Xpress assay 30-7860 on page 3030




Carbonylated Protein ELISA 30-7822156.25–2,500 pmol/mL

156.25 pmol/mL

4 µL

Biological Fluids, Bronchial Lavage, Cell Culture, Cerebrospinal Fluid, Plasma

4h

Ceruloplasmin Rat ELISA 41-CERRT-E0118.75–600 ng/mL

18.75 ng/mL 5 µL Serum 2h 10m

CETP ELISA 47-CETHU-E01 0.2–5 µg/mL 0.2 µg/mL 10 µL Plasma, Serum 3h 15m

Creatine Kinase ELISA 25-CKMHU-E01 7.5–200 ng/mL 7.5 ng/mL 20 µL Serum 1h 20m

CRF-HS Mouse/Rat ELISA 48-CRFMS-E01 0.078–2.5 ng/mL 0.078 ng/mL 50 µL Plasma, Tissue Culture 8h 30m

CRP Canine ELISA 41-CRPCA-E012.125–200 ng/mL

5 ng/mL 5 µL Plasma, Serum 25m

CRP ELISA 30-9710s 1.9–150 ng/mL 0.124 ng/mL 10 µLPlasma, Serum, Stool, Urine

2h 15m

CRP Equine ELISA 41-CRPEQ-E01 10–320 ng/mL 10 ng/mL 5 µL Plasma, Serum 1h 15m

CRP Feline ELISA 41-CRPFE-E013.125–200 ng/mL


CRP Mouse ELISA 41-CRPMS-E01 0.39–25 ng/mL 0.39 ng/mL 5 µL Plasma, Serum 25m

CRP Rabbit ELISA 41-CRPRB-E01 6.25–200 ng/mL 5 ng/mL 2 µL Plasma, Serum 25m

CRP Rat ELISA 41-CRPRT-E01 6.25–200 ng/mL 5 ng/mL 5 µL Plasma, Serum 25m

DAO REA 30-8220 2.1–80 U/mL 0.2 U/mL 100 µL Plasma, Serum 2h 30m

Endostatin ELISA 04-BI-20742 0–80 nmol/L 0.2 nmol/L 10 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Serum

4h 30m

Endothelin Big ELISA 04-BI-20082H 0–3 pmol/IL 0.02 pmol/IL 50 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

5h 30m

Endothelin ELISA (1-21) 04-BI-20052 0.02–10 fmol/mL 0.02 fmol/mL 50 µLCell Culture, Plasma, Saliva, Serum, Urine

Overnight

Erythropoietin ELISA 21-EPOHU-E01 * 3–500 mIU/mL 0.6 mIU/mL 400 µL Serum 2h 30m

Ferritin ELISA 25-FERHU-E01 * 15–1,000 ng/mL 5 ng/mL 20 µL Serum 1h 5m

Ferritin Mouse ELISA 41-FERMS-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Serum 1h 20m

Ferritin Rat ELISA 41-FERRT-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Biological Fluids 1h 20m

Fetuin-A ELISA 43-FETHU-E01 * 0.5–3.7 ng/mL 5 ng/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h

Fetuin-A Ultrasensitive ELISA

43-FETHUU-E01 * 0.01–1.30 g/L 40 ng/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture

2h 45m

Fibrinogen Canine ELISA 41-FIBCA-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

31



Fibrinogen ELISA 41-FIBHU-E01 12.5–400 ng/mL 12.5 ng/mL 5 µLBiological Fluids, Plasma, Serum

1h 40m

Fibrinogen Mouse ELISA 41-FIBMS-E01 25–800 ng/mL 25 ng/mL 5 µLCitrate Plasma, Plasma, Serum

1h 40m

Fibrinogen Porcine ELISA 41-FIBPO-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 15m

Fibrinogen Rat ELISA 41-FIBRT-E01 25–800 ng/mL 25 ng/mL 5 µLCitrate Plasma, Plasma, Serum

1h 40m

Fibronectin ELISA 61-FIBHU-E01 0.31–20 ng/mL 0.1 ng/mL 50 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum, Urine

3h 10m

Fibronectin Mouse ELISA 41-FNEMS-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 40m

Fibronectin Rat ELISA 41-FNERT-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 40m

Galectin-3 ELISA 61-GALHU-E01 0.39–25 ng/mL 0.12 ng/mL 50 µLCell Culture, EDTA Plasma, Serum

3h 10m

Glutathione HPLC (GSH/GSSG)

30-1800 620–970 µmol/L 6 µmol/L 100 µL EDTA Plasma, Plasma See Protocol

GPx Activity Assay 30-7031 70 mg/mL See Protocol 20 µL Biological Fluids 1h

GST Activity Assay 30-2631 See Protocol See Protocol 100 µL Biological Fluids 1h

GST-pi ELISA 30-7960 9.4–600 ng/mL 9 ng/mL 25 µL Serum, Tissue Culture Overnight

Haptoglobin Bovine ELISA 41-HAPBO-E0115.6–1,000 ng/mL

15.6 ng/mL 5 µL Plasma, Serum 40m

Haptoglobin Canine ELISA 41-HAPCA-E01 7.81–125 ng/mL 5 ng/mL 5 µL Plasma, Serum 40m

Haptoglobin ELISA 41-HAPHU-E013.125–200 ng/mL


Haptoglobin Equine ELISA 41-HAPEQ-E0118.75–600 ng/mL


Haptoglobin Feline ELISA 41-HAPFE-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

Haptoglobin Mouse ELISA 41-HAPMS-E01 7.81–125 ng/mL 5 ng/mL 5 µL Plasma, Serum 35m

Haptoglobin Porcine ELISA 41-HAPPO-E01 25–400 ng/mL 5 ng/mL 5 µL Plasma, Serum 40m

Haptoglobin Rabbit ELISA 41-HAPRB-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 30m

Haptoglobin Rat ELISA 41-HAPRT-E01 7.81–125 ng/mL 5 ng/mL 5 µL Plasma, Serum 35m

Hemoglobin ELISA 30-7816A 3.3–100 ng/mL 1.8 ng/mL 100 mg Stool 2h 20m

Hemoglobin Equine ELISA 41-HEMEQ-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h

Hemoglobin Mouse ELISA 41-HEMMS-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 40m

Hemoglobin Rat ELISA 48-HEMRT-E01 5–160 ng/mL 5 ng/mL 100 µL Urine 3h

Hemoglobin Rat ELISA 41-HEMRT-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 30m

32




Hemopexin ELISA 41-HMPHU-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Serum 1h 10m

Hemopexin Mouse ELISA 41-HMPMS-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Serum 1h 30m

Hemopexin Rat ELISA 41-HMPRT-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Serum 1h 20m

Hepcidin 25 LC-MS 30-KM4000 See Protocol 1 ng/mL See Protocol Serum See Protocol

Hepcidin ELISA (25 C-Terminal)

20-HCTHU-E01 2–80 ng/mL 2 ng/mL 20 µL Plasma, Serum 1h 50m

Hepcidin Prohormone ELISA

20-HEPHU-E01 10–1,000 ng/mL 4 ng/mL 50 µL Serum, Urine 3h 30m

Homocysteine ELISA 66-HCYHU-E01 2–50 µmol/L 1 µmol/L 25 µL EDTA Plasma, Serum See Protocol

Homocysteine HPLC 30-2801 See Protocol 0.6 mol/L 50 µL Plasma 1h 50m

Krytopyrrole Colorimetric Assay

30-7823100–1,500 ng/mL

100 ng/mL 1 mL Urine 2h

LCAT ELISA 47-LCAHU-E01 0.14–35 µg/mL 0.14 µg/mL 10 µL Plasma, Serum 3h 15m

Lipoprotein (a) ELISA 55-LPAHU-E01 10–70 mg/dL < 5 mg/dL 5 µL Plasma, Serum 2h 30m

LPL ELISA 47-LPLHU-E01 9–500 ng/mL 9 ng/mL 50 µL Plasma, Serum 4h 15m

Malondialdehyde HPLC 30-1900 See Protocol 0.15 nmol/L 20 µL Plasma, Serum, Urine 1h

MPO ELISA 30-6630 1.2–100 ng/mL 1.6 ng/mL 100 mg Stool, Urine 2h 20m

MPO ELISA (Serum and Plasma)

30-6631A 1.9–30 ng/mL 1.08 ng/mL 10 µL Plasma, Serum 3h 20m

MPO Mouse ELISA 41-MPOMS-E01 0.31–20 ng/mL 0.31 ng/mL 5 µL Plasma, Serum 1h 30m

MRP 8/14 ELISA 01-EK-MRP8/14 0.4–24 µg/mL 0.4 µg/mL 10 µL Plasma, Serum 1h 15m

Myoglobin ELISA 25-MYOHU-E01 * 25–1,000 ng/mL 25 ng/mL 20 µL Serum 1h 5m

Myoglobin ELISA 24-MYOHU-E01 0–250 ng/mL 6.25 ng/mL 10 µL Serum, Urine 30m

Myoglobin Mouse ELISA 41-MYOMS-E01 4.69–300 ng/mL 4.69 ng/mL 30 µL Plasma, Serum 1h 40m

Myoglobin Rat ELISA 41-MYORT-E013.125–200 ng/mL


Nitrotyrosine ELISA 30-7824 32–1,250 nM 34.1 nM 100 mg Plasma, Serum, Stool 4h

NOS1 ELISA 48-NOSHU-E01 0.133–32.4 nM 0.133 nM 50 µL Tissue Culture Overnight

Nt-proCNP ELISA 04-BI-20872 2–40 pmol/L 0.55 pmol/L 50 µLCell Culture, Plasma, Serum

4h 30m

Osteoprotegerin ELISA 04-BI-20403 25–400 pg/mL 1.4 pg/mL 20 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Serum

5h 30m

Osteoprotegerin Mouse/Rat ELISA

30-1020125–4,000 pg/mL

125 pg/mL 100 µLCell Culture, Plasma, Serum, Urine

Overnight

33



Oxidized LDL Antibody ELISA

04-BI-20032 37–1,200 mU/mL 27 mU/mL 50 µL Serum 2h 20m

Oxidized LDL ELISA 30-7810 9–250 ng/mL 9 ng/mL 10 µLEDTA Plasma, Plasma, Serum

2h 20m

OxyStat ELISA 04-BI-5007Cut-off: See Protocol

7 µmol/L 10 µL Plasma, Serum 15m

Phospholipase 2 RIA 48-PLAHU-R50100–10,000 ng/dL

100 ng/dL 50 µL Plasma, Serum 2h 30m

Plasminogen Canine ELISA 41-PLACA-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 45m

Plasminogen ELISA 41-PLAHU-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Serum 2h 5m

Plasminogen Mouse ELISA 41-PLAMS-E016.25–1,900 ng/mL


Plasminogen Porcine ELISA 41-PLAPO-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

Plasminogen Rabbit ELISA 41-PLARB-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 30m

Plasminogen Rat ELISA 41-PLART-E013.125–200 ng/mL


proANP ELISA (1-98) 04-BI-20892 0.63–10 nmol/L 0.05 nmol/L 10 µLCell Culture, Plasma, Urine

4h

Renin Active RIA 72-RENINE * 0–320 pg/mL 1 pg/mL 300 mL Plasma 3h

Renin Activity ELISA 11-RENHU-E02 0.2–60 ng/mL 0.038 ng/mL 50 µL EDTA Plasma, Plasma 1h 45m

ReninRenin is a constituent of the Renin-Angiotensin-Aldosterone System (RASS) which controls blood pressure, renal blood

flux, glomerular filtration, and sodium/potassium homeostasis.1,2 Plasma renin is a reliable index for the activity of the

RAAS. In cases of dysfunction, renin testing with ALPCO’s FDA registered Active Renin RIA can aid in detection of

renin producing tumors, monitoring of glucocorticoid therapy and antihypertensive treatments and contribute to the

diagnosis of hypertension, hyperaldosteronism, and mineral corticoid deficiency.

1 Basso N, Terragno NA. History about the discovery of the renin-angiotensin system. Hypertension. Dec 2001. Vol 38 No 6: 1246–1249.

2 Chappell MC. Nonclassical Renin-Angiotensin system and renal function. Compr Physiology. Oct 2012; Vol 2 No 4: 2733-2752.

View renin assays 11-RENHU-E02 on page 34, and 20-RENHU-E01 and 11-RENHU-L02 on page 3534




Renin Activity LIA 11-RENHU-L02 0.2–25 ng/mL 0.08 ng/mL 500 µL EDTA Plasma, Plasma 1h 30m

Renin ELISA 20-RENHU-E01 0.81–28 pg/mL 0.81 pg/mL 50 µL EDTA Plasma 3h 15m

Serum Amyloid A ELISA 41-SAAHU-E01 0.157–10 ng/mL 0.157 ng/mL 10 µL Plasma, Serum 1h

Serum Amyloid A Mouse ELISA

41-SAAMS-E0131.25–2,000 ng/mL


t-PA ELISA 61-TPAHU-E0115.6–1,000 pg/mL

6 pg/mL 10 µL Cell Culture, Serum 2h 10m

Transferrin Canine ELISA 41-TRACA-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

Transferrin ELISA 41-TRAHU-E019.375–600 ng/mL


Transferrin Mouse ELISA 41-TRAMS-E01 1.56–100 ng/mL 1.56 ng/mL 5 µL Plasma, Serum 1h 10m

Transferrin Rabbit ELISA 41-TRARB-E013.125–100 ng/mL


Transferrin Rat ELISA 41-TRART-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Biological Fluids 40m

Troponin I ELISA 25-TR1HU-E01 * 1.5–75 ng/mL 1 ng/mL 100 µL Serum 1h 50m

Ubiquinone Coenzyme Q10 HPLC Kit

30-KC-1700 See Protocol 0.02 µg/mL 200 µLPlasma, Serum, Whole Blood

15m

Urotensin II ELISA 30-101332–20,000 pg/mL

32 pg/mL 150 µLCell Culture, Plasma, Serum

4h 10m

Vasopressin Direct RIA (ADH/AVP)

01-RK-VPD ^ 1.25–80 pg/mL 0.75 pg/mL 1 mL EDTA Plasma Overnight

Vasopressin Ultrasensitive RIA (ADH/AVP)

01-RK-AR1 0.63–80 pg/mL 0.4 pg/mL 1 mL EDTA Plasma, Urine Overnight

Vitamin A and Vitamin E HPLC Assay (Retinol and Tocopherol HPLC Assay)

30-1600 See Protocol 0.05 mg/mL 100 µL EDTA Plasma, Serum See Protocol

Vitamin B6 Enzymatic Assay

01-KK-VB6 9–250 nM 7 nM 25 µL EDTA Plasma 45m

Vitamin B6 HPLC 30-2100 4.3–17.9 ng/mL 0.2 ng/mL 200 µLEDTA Plasma, Plasma, Serum

See Protocol

35


Diabetes & Obesity

36


According to the 2011 National Diabetes Fact Sheet, diabetes affects 8.3% of the

U.S. population. It is the leading cause of kidney failure, nontraumatic lower-limb

amputations and new cases of blindness among adults and is a major cause of heart

disease and stroke.1 Globally, diabetes affects 347 million people, with Type 2 diabetes

accounting for approximately 90% of all diagnosed cases2 and is projected to be the

7th leading cause of death in 2030.3 Additionally, obesity is a major risk factor for Type

2 diabetes, and having excess visceral, or abdominal, fat is associated with more than a

twofold-increased risk of developing diabetes.4

With no cure for Type 1 or Type 2 diabetes, and a lack

of effective obesity treatments, investigators are

continually in need of new methods to advance their

research. ALPCO has been a leader in offering solutions

1 Centers for Disease Control and Prevention. National diabetes fact sheet: national estimates and general information on diabetes and prediabetes in the

United States, 2011. Atlanta, GA: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, 2011.

2 World Health Organization. Diabetes Fact sheet N°312. Geneva, World Health Organization, 2013.

3 Global status report on noncommunicable diseases 2010. Geneva, World Health Organization, 2011.

4 de Lemos JA, et al. Dysfunctional adiposity and the risk of prediabetes and type 2 diabetes in obese adults. JAMA. Sep 2012; Vol 308 No 11: 1150-1159.

to researchers in the Diabetes & Obesity field and will

continue to support their efforts in gaining a greater

understanding of these conditions.

Diabetes & Obesity

37

Energy Homeostasis

Energy homeostasis is a well-regulated process

that depends on the coordination between feeding

behavior and energy expenditure. The control of energy

homeostasis in humans has received much attention in

recent years due to alterations caused by the onset of

conditions such as obesity and diabetes.

ALPCO offers numerous Research Use Only and

IVD assay kits to assist both academic and industry

researchers with quantification of analytes associated

with energy homeostasis. It is now recognized that there

are many central and peripheral factors involved in

energy homeostasis; understanding of these mechanisms

should lead to effective treatments for the control of

diabetes, obesity and other metabolic disorders.

STELLUX™ Chemiluminescence

Our commitment to support innovation in the Diabetes

and Obesity field has allowed us numerous opportunities

to respond to the changing demands in the market.

ALPCO’s STELLUX™ chemiluminescent detection system

is a result of that commitment.

STELLUX™ is a glow-based chemiluminescent

reaction that delivers rapid and sensitive detection of

analytes, broad dynamic ranges and shorter run times

compared to colorimetric ELISA systems. STELLUX™

chemiluminescence immunoassays will prove to set new

standards in the field of diabetes and obesity research.


AAI RIA 72-AAI See Protocol See Protocol 50 µL Plasma, Serum 2h 30m

Acylated Ghrelin ELISA 32-5106 1.95–250 pg/mL 0.8 pg/mL 100 µL Plasma 3h

Adiponectin HMW & Total ELISA

47-ADPHU-E01 0.075–4.8 ng/mL 0.019 ng/mL 10 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

3h

Adiponectin HMW & Total Mouse ELISA

47-ADPMS-E01 0.125–8 ng/mL 0.032 ng/mL 5 µLEDTA Plasma, Plasma, Serum

3h

Adiponectin Rat ELISA 22-ADPRT-E01 0.25–10 ng/mL 0.08 ng/mL 5 µL Plasma, Serum 2h 30m

Adiponectin Total ELISA 47-ADPHUT-E01 0.075–4.8 ng/mL 0.019 ng/mL 10 µLEDTA Plasma, Heparin Plasma, Serum

2h 40m

AFABP ELISA 32-5181 0.5–25 ng/mL 0.1 ng/mL 20 µLCell Culture, Plasma, Serum

2h 30m

Diabetes & Obesity

38



Albumin Canine ELISA 41-ALBCA-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 45m

Albumin ELISA 30-6330 12.5–800 µg/L 12.5 µg/L 100 mg, 10 µL Stool, Urine 2h 20m

Albumin Monkey ELISA 41-ALBMK-E01 12.5–400 ng/mL 12.5 ng/mL 100 µL Serum, Tissue Culture 1h 40m

Albumin Mouse ELISA 41-ALBMS-E01 7.8–500 ng/mL 5 ng/mL 2 µL Plasma, Serum, Urine 1h 10m

Albumin Porcine ELISA 41-ALBPO-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum, Urine 1h 40m

Albumin Rat ELISA 41-ALBRT-E01 6.25–400 ng/mL 5 ng/mL 5 µL Plasma, Serum, Urine 1h 10m

Anti-Insulin IgG ELISA 35-INAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

CCK RIA 13-CCKHU-R100 0.78–25 pmol/L 0.3 pmol/L 1 mL Plasma Overnight

C-Peptide ELISA 80-CPTHU-E01.1 * 20–3,000 pmol/L 2.95 pmol/L 25 µL Plasma, Serum 2h

C-Peptide ELISA Jumbo 80-CPTHU-E10 * 20–3,000 pmol/L 2.95 pmol/L 25 µL Plasma, Serum 2h

C-peptide ELISA Mouse Jumbo

80-CPTMS-E10 60–3,000 pM 7.6 pM 10 µL Serum 2h 15m

C-Peptide IRMA 72-C-Peptide See Protocol 0.036 ng/mL 50 µL Plasma, Serum, Urine 2h

C-peptide Mouse ELISA 80-CPTMS-E01 60–3,000 pM 7.6 pM 10 µL Serum 2h 15m

C-peptide Rat ELISA 80-CPTRT-E01 50–4,500 pM 10.8 pM 10 µL Serum 2h 15m

FGF-21 ELISA 62-FGFHU-E01 30–1,920 pg/mL 10.4 pg/mL 100 µLCell Culture, Plasma, Serum

2h 35m

FGF-21 Mouse ELISA 62-FGFMS-E0131.2–2,000 pg/mL

10.1 pg/mL 100 µLCell Culture, Plasma, Serum

2h 35m

GAD ELISA 21-GADHU-E01 0.6–2.99 U/mL See Protocol 10 µL Serum 2h 30m

Gastrin RIA 13-MD 302 * 15.6–500 pmol/L 5 pmol/L 100 µL Serum 1h 45m

Gastrin RIA 72-GASK-PR 0–500 µU/mL 10 µU/mL 100 µL Plasma, Serum 3h

Ghrelin Acylated Mouse/Rat ELISA

32-5117 1.95–250 pg/mL 0.7 pg/mL 100 µL Plasma 3h

Ghrelin Non-Acylated ELISA

32-5119 1.95–250 pg/mL 0.6 pg/mL 10 µL EDTA Plasma 3h

Ghrelin Non-Acylated Mouse/Rat ELISA

32-5118 1.95–250 pg/mL 0.8 pg/mL 10 µL EDTA Plasma 3h

Ghrelin Total RIA 22-GHR-R90200–6,400 pg/mL

40 pg/mL 100 µL Plasma, Serum Overnight

Glicentin Rat ELISA 48-GLIRT-E010.206–50 pmol/mL

0.206 pmol/mL 30 µL Plasma Overnight

GLP-1 Active ELISA (7-36) 43-GP1HU-E01 0.64–48 pmol/L 0.05 pmol/L 100 µL EDTA Plasma, Plasma Overnight

GLP-1 Total ELISA (7-36 and 9-36)

43-GPTHU-E01 1.8–54.5 pmol/L 0.6 pmol/L 100 µL EDTA Plasma, Plasma Overnight

39



GLP-2 ELISA 48-GP2HU-E01.10.412–100 ng/mL

0.412 ng/mL 25 µL Plasma, Serum Overnight

GLP-2 Mouse ELISA 48-GP2MS-E010.412–100 ng/mL

0.412 ng/mL 25 µL Plasma, Serum Overnight

GLP-2 Rat ELISA 48-GP2RT-E01 0.137–100 ng/mL 0.137 ng/mL 25 µL Plasma, Serum Overnight

Glucagon ELISA 48-GLUHU-E0150–10,000 pg/mL

50 pg/mL 50 µL Plasma Overnight

Glucagon RIA 13-GLUHU-R100 * 4.7–150 pmol/L 3 pmol/L 200 µL Plasma Overnight



IL-1ß Rat ELISA 61-I1BRT-E0131.3–2,000 pg/mL



3h


3h




3h 30m


IL-8 ELISA 61-IL8HU-E0115.6–1,000 pg/mL

2 pg/mL 50 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

3h 10m

IL-8 Monkey ELISA 61-IL8MK-E01 19.5–1,250 U/mL 3 U/mL 50 µL Cell Culture, Serum 3h 10m

IL-8 Porcine ELISA 45-IL8PO-E0131.2–2,000 pg/mL

10 pg/mL 50 µL Cell Culture, Serum 4h

IL-8 Ultrasensitive ELISA 45-IL8HUU-E01 0.39–25 pg/mL 0.1 pg/mL 100 µLCell Culture, Plasma, Serum, Urine

4h

Insulin Autoantibody ELISA 21-IAAHU-E01Cut-off: See Protocol


Insulin Bovine ELISA 80-INSBO-E01 0.15–6 ng/mL 0.10 ng/mL 25 µL Plasma, Serum 2h 15m

Insulin ELISA 80-INSHU-E01.1 * 3–200 µIU/mL 0.399 µIU/mL 25 µL Plasma, Serum 2h

Insulin ELISA Jumbo 80-INSHU-E10.1 * 3–200 µIU/mL 0.399 µIU/mL 25 µL Plasma, Serum 2h

Insulin High Range Mouse ELISA

80-INSMSH-E01 3–150 ng/mL 1.99 ng/mL 5 µL Plasma, Serum 2h 15m

Insulin High Range Mouse ELISA Jumbo

80-INSMSH-E10 3–150 ng/mL 1.99 ng/mL 5 µL Plasma, Serum 2h 15m

Insulin High Range Rat ELISA

80-INSRTH-E01 3–150 ng/mL 0.52 ng/mL 5 µL Plasma, Serum 2h

Diabetes & Obesity

40



Insulin High Range Rat ELISA Jumbo

80-INSRTH-E10 3–150 ng/mL 0.52 ng/mL 5 µL Plasma, Serum 2h

Insulin Mouse ELISA 80-INSMS-E01 0.188–6.9 ng/mL 0.121 ng/mL 10 µL Plasma, Serum 2h

Insulin Mouse ELISA Jumbo 80-INSMS-E10 0.188–6.9 ng/mL 0.121 ng/mL 10 µL Plasma, Serum 2h

Insulin Ovine ELISA 80-INSOV-E01 0.15–4 ng/mL 0.14 ng/mL 25 µL EDTA Plasma, Serum 2h 15m

Insulin Porcine/Canine ELISA

80-INSPO-E01 0.05–2 ng/mL 0.007 ng/mL 25 µL EDTA Plasma, Serum 2h 15m

Insulin Rat ELISA 80-INSRT-E01 0.15–5.5 ng/mL 0.124 ng/mL 10 µL Plasma, Serum 2h

Insulin Rat ELISA Jumbo 80-INSRT-E10 0.15–5.5 ng/mL 0.124 ng/mL 10 µL Plasma, Serum 2h

Insulin STELLUX™ Rodent ELISA

80-INSMR-CH01 0.1–150 ng/mL 0.096 ng/mL 5 µL Plasma, Serum 2h

Insulin Ultrasensitive ELISA 80-INSHUU-E01.1 * 0.15–20 µIU/mL 0.135 µIU/mL 25 µL Plasma, Serum 1h 30m

Insulin Ultrasensitive Mouse ELISA

80-INSMSU-E01 0.025–6.9 ng/mL0.115 ng/mL (5 µL), 0.019 ng/mL (25 µL)

5 µL, 25 µL Plasma, Serum 2h 30m

Insulin Ultrasensitive Mouse ELISA Jumbo

80-INSMSU-E10 0.025–6.9 ng/mL0.115 ng/mL (5 µL), 0.019 ng/mL (25 µL)


Insulin Ultrasensitive Rat ELISA

80-INSRTU-E010.15–5.5 ng/mL or 0.02–1 ng/mL

0.107 ng/mL (5 µL), 0.002 ng/mL (25 µL)


GlucagonGlucagon is the counter-regulatory hormone to insulin

and is secreted from pancreatic alpha-cells in response

to low blood glucose. Hyperglucagonemia has been

implicated in the pathophysiology of Type 2 Diabetes

Mellitus. Incretin-based treatments (GLP-1 receptor

agonists, DPP-4 inhibitors) have been shown to suppress

glucagon secretion from the alpha-cells, attributing to the

glucose-lowering effect of these treatments.1 As such, the

inhibition of glucagon may prove to be a viable target for

new drug therapies.

1 Morten Hansen, Kristine J. Hare, Jens J. Holst and Filip K. Knop. Inhibition of Glucagon Secretion by GLP-1 Agonists and DPP4 Inhibitors JCMD. Jun 2011; Vol 2

No 1: 7-13.

View glucagon assays 48-GLUHU-E01 and 13-GLUHU-R100 on page 40

41



Insulin Ultrasensitive Rat ELISA Jumbo

80-INSRTU-E100.02–1 ng/mL or 0.15–5.5 ng/mL

0.01 ng/mL (5 µL), 0.07 ng/mL (25 µL)


Islet Cell Antibody ELISA 21-ICAHU-E01Cut-off: See Protocol


Leptin ELISA 11-LEPHU-E01 1–100 ng/mL 0.42 ng/mL 20 µL Serum 1h 45m

Leptin Mouse/Rat ELISA 22-LEPMS-E01 15–1,600 pg/mL 10 pg/mL 5 µL Serum 3h

Leptin RIA (Coated Tube) 22-LEP-R44 1–64 ng/mL 0.1 ng/mL 25 µLCell Culture, Plasma, Serum

Overnight

Leptin Ultrasensitive ELISA 22-LEP-E077 0.05–5 ng/mL 0.01 ng/mL 10 µLBiological Fluids, Plasma, Serum

1h 45m

Leptin Ultrasensitive RIA 22-LEP-R40 0.25–16 ng/mL 0.04 ng/mL 33 µLCell Culture, Cerebrospinal Fluid, Plasma, Serum, Urine

Overnight

MCP-1 ELISA 61-MCPHU-E0115.6–1,000 pg/mL

2.3 pg/mL 20 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

2h 10m

MCP-1 Mouse ELISA 61-MCPMS-E0115.6–1,000 pg/mL

2.2 pg/mL 50 µL Cell Culture, Serum 3h 10m

MCP-1 Rat ELISA 45-MCPRT-E01 11.7–750 pg/mL 8 pg/mL 50 µLCell Culture, Plasma, Serum

2h 30m

Microalbumin ELISA 24-MABHU-E01 0.5–100 µg/mL 0.24 µg/mL 10 µL Urine 40m

AdiponectinAdiponectin circulates as multiple isoforms and is widely

recognized as a key protein involved in insulin regulation

and metabolic syndrome. Measurement of high molecular

weight (HMW) levels, or the ratio of HMW to total

adiponectin, has been shown to aid in predicting insulin

resistance and the development of metabolic syndrome.1

Our HMW & Total Adiponectin ELISAs were developed

for the selective quantification of human or mouse

adiponectin oligomers using a patented protease

pretreatment. These ELISAs directly measure both HMW

and total adiponectin, respectively.

1 Swarbrick MM, Austrheim‐Smith IT, Stanhope KL, et al. Circulating concentrations of high‐molecular‐weight adiponectin are increased following Roux‐en‐Y

gastric bypass surgery. Diabetologia. Nov 2006; Vol 49 No 11: 2552‐2558.

View adiponectin assays 47-ADPHU-E01, 47-ADPMS-E01, 22-ADPRT-E01, and 47-ADPHUT-E01 on page 38

Diabetes & Obesity

42




NGAL Canine ELISA 54-043 4–400 pg/mL 0.56 pg/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h 10m


3h 10m

NGAL ELISA 54-036 10–1,000 pg/mL 4 pg/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h 10m

NGAL Mouse ELISA 54-042 10–500 pg/mL 0.75 pg/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h 10m

NGAL Porcine ELISA 54-044 10–400 pg/mL 1 pg/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h 10m

NGAL Rapid ELISA 54-037 0.2–20 ng/mL 0.008 ng/mL 10 µLEDTA Plasma, Plasma, Serum, Urine

1h

NPY RIA 13-NPYHU-R100 9.4–300 pmol/L 6 pmol/L 200 µL Plasma, Serum 48h

Obestatin ELISA 48-OBEHU-E01 0.231–25 ng/mL 0.231 ng/mL 20 µL Plasma, Serum Overnight

Obestatin Mouse/Rat ELISA 48-OBEMS-E01 0.082–20 ng/mL 0.082 ng/mL 25 µL Serum Overnight

PAI-1 ELISA 61-PAIHU-E01 78–5,000 pg/mL 29 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

Pancreatic Amylase ELISA 30-6410440–28,000 mU/L

440 mU/L 100 mg Stool 2h 15m

Pancreatic Polypeptide RIA 13-PPTHU-R100 6.25–200 pmol/L 3 pmol/L 100 µL Serum Overnight

Pepsinogen I ELISA 43-PE1HU-E01 3–300 ng/mL 0.1 ng/mL 50 µL Serum 1h 20m

Pepsinogen II ELISA 43-PE2HU-E01 3–300 ng/mL 0.1 ng/mL 50 µL Serum 2h 20m

Proinsulin ELISA 80-PINHU-E01.1 * 2.5–180 pM 1.25 pM 50 µL Heparin Plasma, Serum 2h

Proinsulin Mouse ELISA 80-PINMS-E01 4–300 pM 1.5 pM 10 µL Serum 2h 30m

Proinsulin Rat ELISA 80-PINRT-E01 4–300 pM 1.75 pM 10 µL Serum 2h 30m

PYY ELISA 48-PYYHU-E01.1 0.082–20 ng/mL 0.082 ng/mL 50 µL Plasma, Serum Overnight

PYY Mouse/Rat ELISA 48-PYYRT-E01.1 0.15–12.5 ng/mL 0.31 ng/mL 25 µLEDTA Plasma, Plasma, Serum

Overnight

RBP ELISA 30-6110 5.5–165 µg/mL 0.9 µg/L 20 µL Plasma, Serum, Urine 2h 20m

RBP4 Mouse ELISA 41-RBPMS-E01 0.625–20 ng/mL See Protocol 5 µL Plasma, Serum 1h 10m

RBP4 Rat ELISA 41-RBPRT-E01 6.25–200 ng/mL See Protocol 5 µL Plasma, Serum, Urine 1h 30m

43



Resistin Rat ELISA 32-5179 0.25–20 ng/mL 0.05 ng/mL 10 µL Cell Culture, Serum 2h 30m

Resistin Ultrasensitive ELISA

22-RESHUU-E01 20–1,000 pg/mL 6 pg/mL 20 µLCell Culture, Plasma, Serum

4h

Sclerostin ELISA 04-BI-20492 15–240 pM 2.6 pM 20 µLEDTA Plasma, Heparin Plasma, Serum

Overnight

Sclerostin Mouse ELISA 41-SCLMS-E0137.5–1,200 pg/mL

17.4 pg/mL 15 µL Heparin Plasma, Serum 5h 20m



41-SAAMS-E0131.25–2,000 ng/mL


Somatostatin RIA 13-RB 306 0–125 pmol/L 6 pmol/L 1 mL Plasma Overnight

TNF-α ELISA 45-TNFHU-E05 15–1,500 pg/mL 1.7 pg/mL 100 µLCell Culture, Plasma, Serum

4h

TNF-α ELISA 45-TNFHU-E0115.6–1,500 pg/mL


4h

TNF-α Monkey ELISA 61-TNAMK-E01 28.1–1,800 U/mL 6 U/mL 50 µL Cell Culture, Serum 3h 10m

STELLUX™ Rodent Insulin ELISAALPCO’s STELLUX™ Rodent Insulin ELISA, has a

broad dynamic range that significantly reduces sample

preparation time by eliminating dilution steps and the

guesswork involved in determining ideal dilution factors.

The STELLUX™ Rodent Insulin ELISA can be run with

either mouse or rat samples, as it detects isoforms of both

rodent insulin genes. With no cross-reactivity to c-peptide

or proinsulin, this assay’s results are dependable and

accurate.

• Broad range of 0.1 – 150 ng/mL

• Small sample size of 5 µL

• 2 hour incubation

Inter-Lab Correlation Study with

STELLUX™ Rodent Insulin ELISA (r2 value>0.99)

Inter-Lab Correlation Study

0.1 1 10 100 1000

0.1

1

10

100

1000Lab A

Lab B

Lab C

ALPCO Sample Results (ng/mL)

Off

-sit

e La

bo

rato

ry S

amp

le R

esu

lts

(ng/

mL)

View the STELLUX assay 80-INSMR-CH01 on page 41

Diabetes & Obesity

44



TNF-α Mouse ELISA 61-TNFMS-E0115.6–1,000 pg/mL

3 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Serum

4h 10m

TNF-α Porcine ELISA 45-TNFPO-E01.123.4–1,500 pg/mL

4 pg/mL 50 µLCell Culture, EDTA Plasma, Serum

5h

TNF-α Rat ELISA 61-TNART-E0139.1–2,500 pg/mL


TNF-α Ultrasensitive ELISA 45-TNFHUU-E01 0.5–32 pg/mL 0.09 pg/mL 50 µLCell Culture, Plasma, Serum

3h


3h

Vaspin ELISA 22-VASHU-E01 10–1,000 pg/mL 4 pg/mL 70 µL Serum 3h 30m

45


Endocrinology

46


Endocrinology is the field of study which encompasses the functions of the endocrine

system, its complex array of hormones and associated disorders. The endocrine

system is comprised of a vast network of glands and chemically diverse messengers

that interact to regulate coordinated activities with the nervous system in virtually all

tissues. These chemical signaling molecules are called hormones, and they are classified

by their different molecular structures.

Endocrine glands such as sweat,

sebaceous, mucous and digestive,

secrete hormones into ducts which

deliver them to other parts of the

body. At the same time, exocrine

glands like the pituitary, thyroid,

parathyroid and adrenals, secrete

hormones into the extracellular

space and are transported via

capillaries to the blood. Organs that

have an endocrine function include

the pancreas, testes, ovaries,

kidneys, stomach, small intestine,

skin, heart and placenta.

Endocrine System

The endocrine system has five

broad functions:

• Maintain fluid and chemical

homeostasis

• Regulate energy metabolism

and balance

• Govern adaptation to

environmental stimuli such as

trauma and stress

• Integrate coordinated

development

• Support reproductive

processes, from fertilization

to birth and nourishment of

the newborn

Hormones

Hormones fall into three categories:

• Amines, such as the

catecholamines and thyroid

hormones, contain a tyrosine

backbone and are the simplest

type of hormone

• Protein and peptide hormones

are chains of amino acids

of varying length (including

oxytocin and insulin)

• Steroid hormones are derived

from cholesterol, lipid soluble,

and include the reproductive

hormones, glucocorticoids

and mineralcorticoids

Endocrinology

47


1,25 (OH)2 Vitamin D Extraction Kit


17 ß Estradiol ELISA (Saliva) 33-10230 1–100 pg/mL 0.5 pg/mL 100 µL Saliva 2h 15m

17-α-Hydroxy Progesterone ELISA

20-17OHU-E01 * 0.15–20 ng/mL 0.034 ng/mL 25 µL Serum 1h 35m

17-α-Hydroxy Progesterone ELISA (Saliva)

20-17OHU-E01-SLV * 0.005–5 ng/mL 0.003 ng/mL 25 µL Saliva 1h 15m


5a-Androstane-3a, 17b-diol Glucuronide ELISA

11-ANDHU-E01 0.25–50 ng/mL 0.1 ng/mL 50 µL Serum 45m

5a-Androstane-3a, 17b-diol Glucuronide LIA

11-ANDHU-L01 0.25–50 ng/mL 0.1 ng/mL 50 µL Serum 30m

α-1-Antichymotrypsin ELISA

41-A1CHU-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 30m

α-MSH RIA 13-RB 303 4.7–150 pmol/L 3 pmol/L 100 µLCerebrospinal Fluid, Plasma

Overnight

ß Endorphin RIA 13-RB 301 15.6–500 pmol/L 3 pmol/L 3 mL Plasma Overnight

ß hCG Free ELISA 25-FBEHU-E01 2.5–50 ng/mL 0.25 ng/mL 50 µL Serum 1h 20m

ACE Kinetic 01-KK-ACK * ^ 3–150 ACE units 5 ACE Units 25 µL Serum 15m

ACE Kinetic 01-KK-ACK2 * ^ 3–150 ACE Units 5 ACE Units 25 µL Serum 15m

ACE Kinetic Jumbo (1200) 01-KK-ACKX * ^ 3–150 ACE units 5 ACE Units 25 µL Serum 15m

Acid Labile Subunit ELISA 22-ALS-E35 1.5–40 mU/mL 0.23 mU/mL 5 µL Serum 3h


Active Renin RIA 72-RENINE * 0–320 pg/mL 1 pg/mL 300 mL Plasma 3h

AFP ELISA 25-AFPHU-E01 5–300 ng/mL 2 ng/mL 20 µL Serum 1h 20m

Aldosterone ELISA 11-ALDHU-E01 20–2,000 pg/mL 15 pg/mL 100 µL Serum, Urine 1h 15m

Aldosterone LIA 11-ALDHU-L01 20–2,000 pg/mL 4.24 pg/mL 200 µL Serum, Urine 35m

Aldosterone RIA 72-ALDO-RIACT 7–1,500 pg/mL 7 pg/mL200 µL, 200 µL, 10 µL

Plasma, Serum, Urine 3h

Androstenedione ELISA 11-ANRHU-E01 * 0.1–10 ng/mL 0.04 ng/mL 25 µL Serum 1h 15m

Angiotensin II ELISA 32-5880 1–125 pg/mL 1 pg/mL 100 µL Plasma Overnight

Angiotensin II RIA 01-RK-A22 * 2–500 pg/mL 1 pg/mL 1 mL Plasma Overnight

Anti-Sperm Antibody ELISA 20-ASAHU-E01 62–500 U/mL 62 U/mL 10 µL Plasma, Serum 1h 30m

Anti-TG LIA 14-HD-102.1 * 20–2,000 U/mL 8.2 U/mL 20 µL Serum 2h

Endocrinology

48



Anti-TPO LIA 14-HD-103.1 * 30–3,000 U/mL 16.7 U/mL 20 µL Serum 2h

Calcitonin ELISA 21-CALHU-E01 * 10–1,000 pg/mL 1 pg/mL 100 µL Serum 4h 30m

Calcitonin Mouse/Rat IRMA 31-CALRT-R100 12–1,800 pg/mL 0.6 pg/mL 200 µLCell Culture, Plasma, Serum

Overnight

CCK RIA 13-CCKHU-R100 0.78–25 pmol/L 0.3 pmol/L 1 mL Plasma Overnight

Chromogranin A ELISA 43-CHRHU-E01.2 31–830 ng/mL 5 ng/mL 50 µL EDTA Plasma 2h 20m

Chromogranin A RIA 13-RB 321 0.156–5 nmol/L 0.04 nmol/L 50 µL Plasma Overnight

c-Met ELISA (HGFR) 48-CMTHU-E01 0.78–50 ng/mL 0.26 ng/mL 100 µLCell Culture, EDTA Plasma, Plasma, Serum

2h

Corticosterone Mouse/Rat ELISA

55-CORMS-E01 15–2,250 ng/mL 4.5 ng/mL 10 µL Plasma, Serum 2h 30m

Cortisol ELISA 11-CORHU-E01 0.5–60 µg/dL 0.4 µg/dL 20 µL Serum 1h

Cortisol ELISA (Saliva) 11-CORHU-E01-SLV 1–100 ng/mL 1 ng/mL 50 µL Saliva 1h

Cortisol ELISA (Urinary) 33-10110 10–500 ng/mL 10 ng/mL 10 µL Urine 1h 15m

DHEA ELISA 20-DHEHU-E01 * 0.37–30 ng/mL 0.108 ng/mL 10 µL Serum 1h 15m

DHEA-S ELISA 11-DHEHU-E01 * 0.005–10 µg/mL 5 pg/mL 25 µL Serum 1h

DHEA-S Salivary ELISA 33-10250 0.2–12 ng/mL 0.045 ng/mL 50 µL Saliva 30m

DHT ELISA 11-DHTHU-E01 25–2,500 pg/mL 6 pg/mL 50 µL Serum 1h 15m

EPO ELISA 21-EPOHU-E01 * 3–500 mIU/mL 0.6 mIU/mL 400 µL Serum 2h 30m

Estradiol 17 ß ELISA 48-ESTHU-E0116.5–4,000 pg/mL

16.5 pg/mL 100 µL Water Overnight

Estradiol ELISA 11-ESTHU-E01 * 20–3,200 pg/mL 10 pg/mL 50 µL Serum 1h 15m

Estradiol RIA 72-ESTR-CTRIA 8–5,000 pg/mL 8 pg/mL 100 µL Plasma, Serum 4h

Estradiol Ultrasensitive ELISA

20-ESTHUU-E01 * 3–200 pg/mL 3 pg/mL 100 µL Plasma, Serum 4h 30m

Estradiol Ultrasensitive RIA 72-ESTR-US-CT 10–2,000 pmol/L 5 pmol/L 200 µLEDTA Plasma, Heparin Plasma, Plasma, Serum

2h

Estriol ELISA (Saliva) 33-10270 2.5–4,000 pg/mL 1.1 pg/mL 50 µL Saliva 1h 15m

Estriol Free ELISA 20-FE3HU-E01 * 0.3–40 ng/mL 0.075 ng/mL 10 µL Serum 1h 30m

Estrone ELISA 11-ESRHU-E01 * 15–2,000 pg/mL 10 pg/mL 50 µL Serum 1h 15m

Estrone LIA 11-ESRHU-L01 15–2,000 pg/mL 8.8 pg/mL 50 µL Serum 40m

Estrone-3-Sulfate Equine ELISA

55-E3SEQ-E01 5–1,000 ng/mL 0.14 ng/mL 20 µL Serum 30m

Ferritin ELISA 25-FERHU-E01 * 15–1,000 ng/mL 5 ng/mL 20 µL Serum 1h 5m

49



FGF Basic ELISA 45-FGFHU-E0115.6–1,000 pg/mL


4h

FSH ELISA 11-FSHHU-E01 * 5–100 IU/L 1 IU/L 25 µL Serum 1h 20m



Growth Hormone Human ELISA

25-HGHHU-E01 * 2.5–50 ng/mL 0.5 ng/mL 50 µL Serum 1h

Growth Hormone Human RIA

72-HGH-RIACT 0.03–75 µIU/mL 0.03 µIU/mL 50 µL Serum 2h 30m

Growth Hormone Mouse/Rat ELISA

22-GHOMS-E01 0.025–1.5 ng/mL 0.025 ng/mL 10 µL Plasma, Serum 3h

Growth Hormone Rat ELISA 32-5104 0.31–40 ng/mL 0.5 ng/mL 50 µLCell Culture, Plasma, Serum

Overnight

hCG ELISA 25-HCGHU-E01 * 5–300 mIU/mL 2 mIU/mL 50 µL Serum 1h

Hepcidin 25 LC-MS 30-KM4000 See Protocol 1 ng/mL See Protocol Serum See Protocol

Hepcidin ELISA (25 C-Terminal)

20-HCTHU-E01 2–80 ng/mL 2 ng/mL 20 µL Plasma, Serum 1h 50m

IGF-1 ELISAInsulin-like Growth Factor 1 (IGF-1,

Somatomedin C) is one of several

growth factors necessary for normal

human development. ALPCO’s IGF-1

ELISA is an FDA registered kit which

provides results in-line with leading

clinical methods.

IGF-1 is synthesized mainly by the

liver, but also locally in many tissues. In contrast to many other peptide hormones, IGFs are avidly bound to specific

IGFBPs, which significantly increases the half-life of IGF-I in circulation. The seven classes of IGFBPs which are known

at present 1, 2, 3 either bind IGF-I and IGF-II with similar affinities or show a preference for IGF-II. 4, 5 The predominant

measured IGF-I [pg/tube]

actual IGF-I[pg/tube]

10000

1000

100

10

11 10 10 100 1000 10000

conventional

0.5 ng IGFBP-3

ALPCO

measured IGF-I [pg/tube]

actual IGF-I[pg/tube]

10000

1000

100

10

11 10 10 100 1000 10000

conventional

5 ng IGFBP-3

ALPCO

Minimal interference of IGFBP in IGF-I measurements

with ALPCO assay versus conventional method

Endocrinology

50


1 Ballard J, Baxter R, Binoux M, et al. On the nomenclature of the IGF binding proteins. Acta Endocrinology. Nov 1989; Vol 121: 751-752.

2 Drop SLS. Report on the nomenclature of the IGF binding proteins. J Clin Endocrinology & Metabolism. Mar 1990; Vol 70 No 3: 817-818.

3 Wilson EM, Oh Y, Rosenfeld RG. Generation and characterization of an IGFBP-7 antibody: Identification of 31 kD IGFBP-7 in human biological fluids and

Hs578T human breast cancer conditioned media. J Clin Endocrinology & Metabolism. Apr 1997. Vol 82 No 4:1301-1303.

4 Martin JL, Baxter RC. Insulin-like growth factor binding protein from human plasma. Purification and characterization. J Biol Chem. Jul 1986; Vol 261: 8754-8760.

5 Binkert C, Landwehr J, Mary JL, et al. Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein

(IGFBP-2). EMBO J. Sep 1989; Vol8 No9: 2497-2502.

View the IGF-1 ELISA assay 22-IGFHU-E01 on page 51


Hepcidin Prohormone ELISA

20-HEPHU-E01 10–1,000 ng/mL 4 ng/mL 50 µL Serum, Urine 3h 30m

Homocysteine ELISA 66-HCYHU-E01 2–50 µmol/L 1 µmol/L 25 µL EDTA Plasma, Serum See Protocol

HPL ELISA 20-HPLHU-E01 1.25–20 mg/L 0.3 mg/L 10 µL Serum 40m

Human Growth Hormone Ultrasensitive ELISA

22-HGHHUU-E01 * 50–1,000 pg/mL 1.6 pg/mL 10 µL Plasma, Serum 3h 15m

IGF-1 Direct RIA 22-IGF-R20 * 0.156–10 ng/mL 0.02 ng/mL 5 µLCell Culture, Plasma, Serum

Overnight

IGF-1 Direct RIA 22-IGF-R21 * 0.156–10 ng/mL 0.02 ng/mL 5 µLCell Culture, Plasma, Serum

Overnight

IGF-1 Direct RIA (Coated Tube)

22-IGF-R22 * 0.37–30 ng/mL 0.1 ng/mL 10 µL Plasma, Serum Overnight

IGF-1 ELISA 22-IGFHU-E01 * 2–50 ng/mL 0.09 ng/mL 10 µL Plasma, Serum 1h 45m

IGF-1 Mouse/Rat ELISA 22-IG1MS-E01 0.5–18 ng/mL 0.029 ng/mL 5 µL Plasma, Serum 2h

IGF-1 RIA 72-IGF1-RIACT 0–835 ng/mL 1 ng/mL 25 µL Plasma, Serum 4h 30m

IGFBP is IGFBP-3, which binds not only IGF-I but also the

Acid-labile Subunit. Thus, most of the IGF-I in circulation

is bound within this ternary complex. IGFBPs can make

the measurement of IGF-I very difficult, because if the

right methodology is not used, the IGFBPs become

interferents in the assay. With ALPCO’s IGF-1 ELISA,

there is no interference from IGFBP-3 so values can be

trusted, and it is traceable to international reference

material of the WHO 02/254.

51



IGF-2 ELISA 22-IGF-E30 0.45–9 ng/mL 0.02 ng/mL 5 µLCerebrospinal Fluid, Plasma, Serum, Stool, Urine

3h

IGF-2 RIA 22-IGF-R30 0.4–50 ng/mL 0.1 ng/mL 10 µLCell Culture, Plasma, Serum

Overnight

IGF-2 RIA 22-IGF-R31 0.4–50 ng/mL 0.1 ng/mL 10 µLCell Culture, Plasma, Serum

Overnight

IGFBP-1 ELISA 11-IGFHU-E01 1–250 µg/L 0.5 µg/L 25 µL Serum 1h 10m

IGFBP-2 ELISA 22-IGF-E05 2–80 ng/mL 0.2 ng/mL 15 µLBreast Milk, Cell Culture, EDTA Plasma, Plasma, Saliva, Serum

1h 45m

IGFBP-2 Mouse/Rat ELISA 22-MIGF-E0831.25–2,000 pg/mL


IGFBP-3 ELISA 22-IGF-E03A 6.6–20 ng/mL 6.6 ng/mL 10 µL Plasma, Serum 2h 30m

IGFBP-3 ELISA (Bioactive) 22-IGF-E04A 0.4–30 ng/mL 2 ng/mL 50 µLEDTA Plasma, Heparin Plasma, Plasma, Serum

3h 30m

IGFBP-3 Mouse/Rat ELISA 22-MIGF-E031 0.078–5 ng/mL 0.018 ng/mL 5 µLCell Culture, Plasma, Serum

2h 30m

IGFBP-3 RIA 22-IGF-R10 0.625–40 ng/mL 0.06 ng/mL 50 µL Plasma, Serum Overnight

IGFBP-3 RIA (Coated Tube) 22-IGF-R11 1–81 ng/mL 0.5 ng/mL 10 µL Plasma, Serum Overnight

Intact PTH Bovine ELISA 31-IPTBO-E01 0–10,000 pg/mL 10 pg/mL 50 µLCell Culture, Plasma, Serum

3h 30m

Luteinizing Hormone ELISA 11-LUTHU-E01 * 1–100 IU/L 0.2 IU/L 25 µL Serum 1h 20m

Lutenizing Hormone ELISA (Urine)

20-LHUHU-E01 10–200 mIU/mL 10 mIU/mL 25 µL Urine 40m


PAPP-A Ultrasensitive ELISA

55-PAPHUU-E0111.25–450 ng/mL

0.023 ng/mL 100 µL Serum 45m

PMSG ELISA 20-PMSHU-E01 See Protocol See Protocol 100 µL Plasma, Serum 2h 30m

Prealbumin ELISA 30-6331 1.6–100 ng/mL 3 ng/mL 100 mg Plasma, Serum, Stool 2h 15m

Prealbumin Mouse ELISA 41-PALMS-E0115.625–500 ng/mL

15.625 ng/mL 5 µL Plasma, Serum 1h

Prealbumin Rat ELISA 41-PALRT-E0115.625–500 ng/mL


Pregnenolone ELISA 11-PREHU-E01 * 0.1–25.6 ng/mL 0.05 ng/mL 50 µL Serum 1h 45m

Progesterone ELISA 11-PROHU-E01 * 0.3–60 ng/mL 0.1 ng/mL 25 µL Serum 1h 10m

Progesterone ELISA (Direct Saliva)

11-PROHU-E01-SLV 20–5,000 pg/mL 20 pg/mL 1 mL Saliva 1h 10m

Progesterone LIA 11-PROHU-L01 0.3–100 ng/mL 0.1 ng/mL 25 µL Serum 35m

Endocrinology

52



Progesterone Mouse/Rat ELISA

55-PROMS-E01 0.4–100 ng/mL 0.04 ng/mL 25 µL Plasma, Serum 1h 30m

Progesterone RIA 72-PROG-CTRIA 0.1–60 ng/mL 0.05 ng/mL 50 µLEDTA Plasma, Heparin Plasma, Plasma, Serum

2h 10m

Prolactin Canine ELISA 55-PRLCA-E01 2.5–80 ng/mL 0.4 ng/mL 25 µL Serum 3h 30m

Prolactin ELISA 25-PROHU-E01 * 5–200 ng/mL 2 ng/mL 50 µL Serum 1h 5m

Prolactin Rat ELISA 55-PRLRT-E01 5–80 ng/mL 0.6 ng/mL 25 µL Serum 3h 30m

PSA Total ELISA 25-PSAHU-E01 2–120 ng/mL 1 ng/mL 50 µL Serum 2h 20m

PTH Bioactive Intact Rat ELISA

31-BIPRT-E01 25–2,700 pg/mL 3 pg/mL 25 µL Cell Culture, Plasma 3h 30m

PTH High Sensitive ELISA (1-34)

31-IPTHUU-E01 3.2–143 pg/mL 0.3 pg/mL 150 µLCell Culture, EDTA Plasma, Plasma

3h 30m

PTH Intact ELISA (1-84) 21-IPTHU-E01 * 7–700 pg/mL 1.57 pg/mL 25 µL Serum 3h 30m

PTH Intact Mouse ELISA (1-84)

31-PTHMS-E0132–1,045 pg/mL or 336–2,616 pg/mL

4 pg/mL 20 µL Cell Culture, Plasma 3h 30m

PTH Intact Porcine ELISA 31-IPTPO-E01 0–1,000 pg/mL 1 pg/mL 50 µLCell Culture, Plasma, Serum

3h 30m

PTH Intact Rat ELISA (1-84) 31-IPTRT-E01 25–2,700 pg/mL 1.6 pg/mL 25 µLCell Culture, Plasma, Serum

3h 30m

Relaxin ELISA 30-9210 4–250 pg/mL 4 pg/mL 100 µLPlasma, Serum, Tissue Culture, Urine

Overnight

Renin Activity ELISA 11-RENHU-E02 0.2–60 ng/mL 0.038 ng/mL 50 µL EDTA Plasma, Plasma 1h 45m

Renin Activity LIA 11-RENHU-L02 0.2–25 ng/mL 0.08 ng/mL 500 µL EDTA Plasma, Plasma 1h 30m

Renin ELISA 20-RENHU-E01 0.81–28 pg/mL 0.81 pg/mL 50 µL EDTA Plasma 3h 15m

Reverse T3 RIA 38-RT3HU-R125 0.025–2 ng/mL 0.009 ng/mL 100 µLAmniotic Fluids, Plasma, Serum

3h

SHBG ELISA 11-SHBHU-E01 3.3–295 nmol/L 0.1 nmol/L 10 µL Serum 1h

Somatostatin RIA 13-RB 306 0–125 pmol/L 6 pmol/L 1 mL Plasma Overnight

T3 Free ELISA 25-FT3HU-E01 0.9–19 pg/mL 0.05 pg/mL 50 µL Serum 1h 20m

T3 Total ELISA 25-TRIHU-E01 * 0.75–10 ng/mL 0.2 ng/mL 50 µL Serum 1h 20m

T3 Total RIA 14-HD-131.100 0.8–10 nmol/L 0.3 nmol/L 50 µL Serum 2h

T4 Free ELISA 25-FT4HU-E01 0.3–7 ng/dL 0.05 ng/dL 50 µL Serum 1h 20m

T4 Free LIA 11-FT4HU-L01 0–120 pg/mL 1 pg/mL 50 µL Serum 45m

T4 Total ELISA 25-TT4HU-E01 * 2–25 µg/dL 0.4 µg/dL 25 µL Serum 1h 20m

T4 Total RIA 14-HD-141.100 25.6–512 nmol/L 12.8 nmol/L 50 µL Serum 2h

53



TBG RIA 14-HD-53.1 5–80 mg/L 5 mg/L 20 µL Serum 2h

Testosterone Direct Saliva ELISA

11-TESHU-E01-SLV 2–800 pg/mL 1 pg/mL 1 mL Saliva 1h 40m

Testosterone ELISA (Serum) 11-TESHU-E01 * 0.08–16.7 ng/mL 0.022 ng/mL 50 µL Serum 1h 10m

Testosterone Free ELISA 11-FTSHU-E01 * 0.25–125 pg/mL 0.17 pg/mL 25 µL Serum 1h 15m

Testosterone Free LIA 11-FTSHU-L01 0.25–100 pg/mL 0.17 pg/mL 25 µL Serum 1h

Testosterone LIA (Saliva) 11-TESHU-L01 2–800 pg/mL 1 pg/mL 1 mL Saliva 1h 10m

Testosterone Mouse/Rat ELISA

55-TESMS-E01 0.1–25 ng/mL 0.066 ng/mL 10 µL Plasma, Serum 30m

Testosterone RIA 72-TESTO-CT2 0.5–50 nmol/L 0.3 nmol/L 25 µL Serum 1h

Thyroglobulin Ab RIA 14-HD-27.1 * 20–2,000 IU/mL 5.5 IU/mL 20 µL Plasma, Serum 2h

Thyroglobulin ELISA 27-GD101 10–400 ng/mL 2 ng/mL 50 µL Serum 1h 40m

Thyroglobulin Plus ILMA 14-HD-111.1 0.15–250 ng/mL 0.02 ng/mL 100 µL Serum Overnight

Thyroglobulin Plus IRMA 14-HD-110.1 * 0.15–250 ng/mL 0.2 ng/mL 100 µL Serum 1h 20m

TPO Ab RIA 14-HD-90.1 * 30–3,000 U/mL 5.5 U/mL 90 µL Serum 1h

TSH Canine ELISA 55-TSHCA-E01 0.2–5.2 ng/mL 0.01 ng/mL 100 µLEDTA Plasma, Plasma, Serum

2h 30m

TSH ELISA 25-TSHHU-E01 * 0.5–25 µIU/mL 0.2 µIU/mL 100 µL Serum 1h 20m

TSH IRMA 14-HD-09.1 0.16–80 MU/L 0.04 mU/L 100 µL Serum 1h

TSH LIA 11-TSHHU-L01 0.008–25 µIU/mL 0.002 µIU/mL 50 µL Serum 1h

TSH Rat ELISA 55-TSHRT-E01 0.1–80 ng/mL 0.1 ng/mL 25 µL Serum Overnight

TSH Receptor Autoantibodies LRA

14-HD-107.1 * 1–40 IU/L 0.4 IU/L 100 µL Serum 3h

TSH Receptor Autoantibodies RIA

14-HD-77.1 5–405 U/L 2.4 U/L 50 µL Serum 2h 15m

TSH RRA Receptor Ab RIA 14-HD-101.1 1–40 IU/L 0.3 IU/L 100 µL Serum 3h

TSH Ultrasensitive ELISA 25-TSHHUU-E01 * 0.1–10 µIU/mL 0.05 µIU/mL 100 µL Serum 2h 20m

Vasoactive Intestinal Peptide RIA

13-RB 311 3.8–120 pmol/L 3 pmol/L 200 µL Serum Overnight

Vasopressin Direct RIA (ADH/AVP)

01-RK-VPD ^ 1.25–80 pg/mL 0.75 pg/mL 1 mL EDTA Plasma Overnight

Vasopressin Ultrasensitive RIA (ADH/AVP)

01-RK-AR1 0.63–80 pg/mL 0.4 pg/mL 1 mL EDTA Plasma, Urine Overnight

Vitamin D2/D3 HPLC 30-3420 4–1,250 nmol/L 4 nmol/L 500 µL Plasma, Serum See Protocol

Endocrinology

54


56

Gastroenterology


Gastroenterology is the branch of medicine that deals with the study and treatment

of the digestive system (comprised of stomach and intestine) and related disorders

affecting it. Gastric diseases and ailments have become a growing therapeutic segment

of interest due to an increased prevalence of irregular dietary habits and poor hygiene

factors across the world.

Calprotectin is considered a reliable indicator of

inflammation in several diseases. Both organic

Inflammatory Bowel Disease (IBD) and non-organic

functional disorders like Irritable Bowel Syndrome

(IBS) exhibit very similar symptoms. Research has

shown the concentration of calprotectin in feces may

be significantly elevated in cases of IBD, whereas

in cases of IBS, levels remain normal. 1,2,3 Thus,

measurement of fecal calprotectin may be useful for

the differential diagnosis and potential management

of organic Inflammatory Bowel Disease. 4,5,6

Measurement of fecal calprotectin levels may provide

physicians with valuable information for timely

decision making on whether to send IBD patients for

colonoscopy, or rather treat them for IBS symptoms.

Traditionally, IBD activity has been assessed through

Calprotectinclinical symptoms, but it has been reported that the

recognized activity indexes are subjective and do not

correlate as consistently as fecal calprotectin levels with

histologic inflammation.7,8

Fecal calprotectin has been demonstrated to be

a reliable biomarker of mucosal inflammation in

several organic IBD pathologies and may function as

a valuable alternative to relying on clinical symptoms,

the use of complex imaging procedures, or performing

endoscopy and/or biopsy. Because of the strong

correlation between calprotectin and disease activity,

many research studies conclude that monitoring fecal

calprotectin levels can be useful for chronicling disease

activity in IBD.7,8,9 Increased levels of calprotectin as an

indicator of inflammation also precedes the appearance

of clinical symptoms.

57

Research has shown that IBD is marked by periods of

remission with intermittent relapses characterized by

increased intestinal inflammation. Thus, monitoring levels

of fecal calprotectin may become essential in determining

the effectiveness of therapy and could become a predictor

of potential relapse. It provides physicians with a valuable

tool, not only for differentiating IBD from IBS cases, but

also allowing them to monitor and predict treatment

outcomes, enabling better management of IBD flares.4-12

1 Røseth AG, Fagerhol MK, Aadland E, Schjønsby H. Assessment of the

neutrophil dominating protein calprotectin in feces. A methodologic study.

Scand J Gastroenterology. Sep 1992; Vol 27 No 9:793-8.

2 Manz M, Burri E, Rothen C, Tchanguizi N, et al. Value of fecal calprotectin

in the evaluation of patients with abdominal discomfort: an observational

study. BMC Gastroenterology. Jan 2012; Vol 12 No 1:5.

Differentiation of IBD vs. IBS

Fecal calprotectin is a reliable surrogate marker for

differentiating organic inflammation (IBD) from functional

symptoms (IBS). 4-12

Predicting Relapse

Calprotectin has been shown in numerous publications to

be a reliable predictor of IBD in remission. 4-12

Disease Monitoring

Low calprotectin levels after treatment (<250 µg/g)

indicate endoscopic mucosal healing. This can assist in

optimizing treatment in IBD patients. 4-12


α-1-Antichymotrypsin ELISA

41-A1CHU-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 30m

α-1-Antitrypsin Mouse ELISA

41-A1AMS-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Serum 2h 5m

α-Fodrin Check IgG/IgA ELISA

35-AFCHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

1,25 (OH)2 Vitamin D ELISA 30-2112 5.1–200 pg/mL 4.8 pg/mL 500 µL Plasma, Serum Overnight



25-OH Vitamin D ELISA 30-2109 2–300 nmol/L 2 nmol/L 30 µL Serum Overnight


58

Gastroenterology



Alpha-1 Thymosin ELISA 30-9510 4–1,000 ng/mL 4 ng/mL 200 µL Serum, Tissue Culture Overnight











3 Røseth AG, Schmidt PN, Fagerhol MK. Correlation between faecal excretion

of indium-111-labelled granulocytes and calprotectin, a granulocyte

marker protein, in patients with inflammatory bowel disease. Scand J

Gastroenterology. Jan 1999; Vol 34 No1:50-4.

4 Tibble J.A., Bjarnason I. et al. Surrogate markers of intestinal inflammation

are predictive of relapse in patients with inflammatory bowel disease.

Gastroenterology Jul 2000; Vol 119 No 1: 15-22.

5 Røseth AG, Aadland E, Grzyb K. Normalization of faecal calprotectin: a

predictor of mucosal healing in patients with inflammatory bowel disease.

Scand J Gastroenterology. Oct 2004; Vol 39 No 10: 1017-1020.

6 Lewis, JD. The utility of biomarkers in the diagnosis and therapy of

inflammatory bowel disease. Gastroenterology. May 2011; Vol 140 No 6:

1817-1826.

7 Burri E, Beglinger C. Faecal calprotectin - a useful tool in the management of

inflammatory bowel disease. Swiss Med Weekly. Apr 2012; Vol 142.

8 D’Haens G, Ferrante M, et al. Fecal calprotectin is a surrogate marker for

endoscopic lesions in inflammatory bowel disease. Inflamm Bowel Disease.

Feb 2012. Vol 18 No 12: 2218-24.

9 Lobatón T, Rodríguez-Moranta F, Lopez A, et al. A new rapid quantitative

test for fecal calprotectin predicts endoscopic activity in ulcerative colitis.

Inflamm Bowel Disease. Apr 2013. Vol 19 No 5: 1034-1042.

10 Louis E, Mary JY, Vernier-Massouille G, et al. Maintenance of remission

among patients with Crohn’s disease on antimetabolite therapy after

infliximab therapy is stopped. Gastroenterology. Jan 2012; Vol 142 No 1:

63-70.

11 De Vos M, Dewit O, D’Haens G, et al. Fast and sharp decrease in calprotectin

predicts remission by infliximab in anti-TNF naïve patients with ulcerative

colitis. J Crohns Colitis. Jun 2012; Vol 6 No 5: 557-62.

12 Sorrentino D, Terrosu G, Paviotti A, et al. Early diagnosis and treatment of

postoperative endoscopic recurrence of Crohn’s disease: partial benefit by

infliximab--a pilot study. Digestive Diseases and Sciences. May 2012; Vol 57

No 5: 1341-1348.

59


View calprotectin assays 01-EK-CAL, 01-LF-CHR25, 01-LF-CAL25, and 01-DS-CAL25 on page 60








Anti-Human Tissue Transglutaminase

sIgA ELISA30-9393


See Protocol 100 mg Stool 2h 30m














Biotin ELISA 30-8140 37.5–600 ng/L 15 ng/L 150 µL Serum, Urine 1h 15m

Biotin Microbiological Assay

30-KIF007 0.02–0.18 µg/L 0.02 µg/L 100 µL Serum Overnight

Calprotectin CALcheck Blue™ Dipstick (25 tests)

01-DS-CAL25Cut-off: See Protocol

See Protocol See Protocol Stool 10m

Calprotectin ELISA 01-EK-CAL ^ † 10–600 µg/g or 30–1,800 µg/g

10 µg/g 50 mg Stool 1h 15m

Calprotectin ELISA (2x96 Wells)

01-EK-CAL-02 ^ 10–600 µg/g or 30–1,800 µg/g


Calprotectin Mouse/Rat ELISA

30-6936 0.25–15.6 ng/mL 0.076 ng/mL 100 µLCell Culture, Plasma, Serum, Stool, Tissue Culture, Urine

4h 20m

Calprotectin Quantum Blue® High Range

Rapid Test01-LF-CHR25 ^ 100–1,800 µg/g 100 µg/g 80 mg Stool 15m

Calprotectin Quantum Blue® Rapid Test

01-LF-CAL25 ^ 30–300 µg/g 10 µg/g 80 mg Stool 12m

Care™ FOB Rapid Testing Kit (20 tests)

43-FOBHU-RP20 * See Protocol0.05 µg h-Hb/mL

See Protocol Stool 5m

Care™ FOB Rapid Testing Kit (50 tests)

43-FOBHU-RP50 * See Protocol0.05 µg h-Hb/mL

See Protocol Stool 6m

Casomorphin ELISA 30-7010 1–120 ng/mL 1 ng/mL 2 mL Urine Overnight

CAST® ELISA (Leukotriene Function)

01-EK-CAST 50–3,200 pg/mL 20 pg/mL 2 mL Whole Blood 2h 30m

60

Gastroenterology

* FDA Registered for In Vitro Diagnostic Use ^ Health Canada Licensed † Pending FDA Clearance

Products are for Research Use Only unless otherwise noted


CAST® ELISA Jumbo (Leukotriene Function)

01-EK-CAST5 50–3,200 pg/mL 20 pg/mL 2 mL Whole Blood 2h 30m

CeliCheck ELISA (Tissue Transglutaminase

IgA/IgG ELISA)35-CELHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h 30m

Chymotrypsin Activity Assay

30-6990 13.2 U/g See Protocol 100 µL Stool 5m

Chymotrypsin ELISA 30-6910 20–1,000 ng/mL 20 ng/mL 100 mg Stool 2h 30m

Crohns Check ELISA (Anti-Saccharomyces

Cerevisiae IgA/IgG ELISA)35-CROHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

DAO ELISA 30-8500 0–15.65 U/mL 0.052 U/mL 50 µL Serum 4h 20m

EDN ELISA 30-6811 0.25–16 ng/mL 0.165 ng/mL 80 mg, 20 µLPlasma, Serum, Stool, Urine

2h 20m

FlowCAST Highsens® High Sensitivity Basophil

Activation Test01-FK-HSBAT ^ See Protocol See Protocol 100 µL Whole Blood 1h

FlowCAST® Basophil Activation Test

01-FK-CCR See Protocol See Protocol 50 µL Whole Blood 30m

Folic Acid Vitamin B9 Microbiological Test Kit

30-KIF005 4–40 µg/L 4 µg/L 100 µL Serum Overnight

Gastrin RIA 13-MD 302 * 15.6–500 pmol/L 5 pmol/L 100 µL Serum 1h 45m

Gastrin RIA 72-GASK-PR 0–500 µU/mL 10 µU/mL 100 µL Plasma, Serum 3h

Gliadorphin ELISA 30-7011 3–30 ng/mL 2 ng/mL 2 mL Urine Overnight

Hemoglobin/Haptoglobin ELISA

30-7817 0.1–3.5 U/L 0.30 U/L 100 mg Stool 2h 20m

Lactoferrin ELISA 41-LACHU-E013.125–100 ng/mL

3.125 ng/mL 5 µL Milk, Plasma, Serum 1h 15m

Lysozyme ELISA 30-6900 1.1–30 ng/mL 0.5 ng/mL 100 mg, 15 mg Stool 2h 20m

Food IntoleranceALPCO offers a number of assays for the measurement of

food-specific proteins such as gliadin, ovalbumin, casein,

and soya. Kits contain reagents which are ready-to-use

and coded to ensure accurate reagent dispensing. The

assays require minimal pipetting steps, making them

convenient for manual processing if needed.

61



Lysozyme ELISA (Serum, Urine, Liquor)

30-6902 1.1–30 ng/ml 0.5 ng/ml 100 µL See Protocol 2h 20m


Niacin MicroBiological Assay Kit (Vitamin B3)


Pepsinogen I ELISA 43-PE1HU-E01 3–300 ng/mL 0.1 ng/mL 50 µL Serum 1h 20m

Pepsinogen II ELISA 43-PE2HU-E01 3–300 ng/mL 0.1 ng/mL 50 µL Serum 2h 20m

q-FOB™ Immunochemical Test (FIT) Kit

43-FOBHU-E01 0–82 ng/mL 2.6 ng/mL 100 µL Stool 40m

Riboflavin Microbiological Assay Kit (Vitamin B2)

30-KIF002 5–60 ng/L 5 ng/L 150 µL Serum Overnight

S100A12 ELISA 30-6938 0.22–6 ng/mL 0.016 ng/mL 100 mg Stool 2h

Secretory IgA ELISA 30-8870 22.2–600 ng/mL 13.4 ng/mL 100 mg, 10 µL Saliva, Stool 2h 10m

Secretory IgA1 ELISA 30-6863 3.3–100 U 3.3 U 100 mg Stool 2h 15m


ß Defensin 2 ELISA 30-6500 0.1–3 ng/mL 0.077 ng/mL 100 mg Stool 3h 30m

Thiamin Microbiological Assay Kit (Vitamin B1)

30-KIF001 3–15 ng/L 3 ng/L 100 µL Whole Blood Overnight

Secretory IgASecretory IgA is a marker of intestinal immune status and

is a major immunoglobulin in gastrointestinal secretions.

Secretory IgA is an important factor in mucosal immunity

and in the intraluminal microbial defense system. It plays

a role in preventing adherence of microorganisms to

mucosal sites, in activation of the alternative complement

pathway and in activating inflammatory reactions.

Measuring levels of Secretory IgA may aid in the

understanding of intestinal immune status. A low sIgA-

level may indicate reduced activity of the intestinal

immune system, while an elevated sIgA-level may be

indicative of immune system activation of the gut mucosa

caused by pathogens or allergic processes.1

1 Yel, L. Selective IgA Deficiency. J Clinical Immunology. Jan 2010; Vol 30 No 1: 10–16.

62

Gastroenterology


View secretory IgA assays 30-8870, 30-6863, and 30-6864 on page 62


Vasoactive Intestinal Peptide RIA

13-RB 311 3.8–120 pmol/L 3 pmol/L 200 µL Serum Overnight

Vitamin A and Vitamin E HPLC Assay (Retinol and Tocopherol HPLC Assay)

30-1600 See Protocol 0.05 mg/mL 100 µL EDTA Plasma, Serum See Protocol

Vitamin B1 HPLC 30-2201 12–192 nmol/L 0.13 nmol/L 50 µL Plasma 1h 35m

Vitamin B12 MicroBiological Test Kit


Vitamin B5 Pantothenic Acid Microbiological Assay


Vitamin B6 Enzymatic Assay

01-KK-VB6 9–250 nM 7 nM 25 µL EDTA Plasma 45m

Vitamin B6 HPLC 30-2100 4.3–17.9 ng/mL 0.2 ng/mL 200 µLEDTA Plasma, Plasma, Serum

See Protocol

Vitamin B6 MicroBiological Assay


Vitamin C HPLC (Ascorbic Acid)

30-2900 4–20 mg/L 0.25 mg/L 200 µLHeparin Plasma, Plasma

See Protocol




41-VDBRT-E013.125–100 ng/mL


Vitamin K1 HPLC 30-2400 See Protocol 0.15 ng/mL 1 mL Plasma, Serum See Protocol

Zonulin ELISA 30-5600 0–1,000 ng/mL 0.6 ng/mL 250 µL Stool 3h 10m

Zonulin ELISA (Serum) 30-5601 See Protocol 0.6 ng/mL See Protocol Serum 3h 10m

63


Immunology

64


Immunology is the branch of biomedical science that is concerned with the relationship

between body systems, pathogens and immunity. The central science of immunology is

the study of the molecular and cellular components that comprise the immune system,

including their function and interaction. ALPCO offers tools that can be used to gain

insight into the development or progression of immune responses.

Innate Immune System

The innate system attempts to

protect the body from all invaders.

The elements of this system include

surface barriers and internal

defenses. The two surface barriers

are skin and mucosae. The skin

provides a nearly impenetrable

barrier to microorganisms and

fluids secreted onto the skin

contain chemicals that inhibit the

growth of microorganisms. Mucous

membranes produce mucus to trap

microorganisms.

The internal defenses consist of cells

and chemicals. The predominant

cell types involved are phagocytes

and natural killer cells. In response

to microorganisms, these cell

types release chemicals and

antimicrobial proteins as a part of

the inflammatory response.

Adaptive Immune System

The adaptive system can initiate

specific attacks against specific

invaders. This system consists of

cells that recognize specific foreign

substances and act to neutralize or

destroy them. Three types of cells

play the major roles in the adaptive

immune system: B lymphocytes (B

cells), T lymphocytes (T cells) and

antigen-presenting cells (APCs).

Following exposure of these cell

types to an antigen, they retain the

ability to recognize the same antigen

in the future which leads to faster

immune system response time.

Innate vs. Adaptive Immune SystemThe immune system is comprised of two subdivisions—the innate immune system (non-specific)

and the adaptive immune system (specific).

Immunology

65


α-1-Acid Glycoprotein Chicken ELISA

41-AGPCH-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

α-1-Acid Glycoprotein ELISA

41-AGPHU-E01 5–320 ng/mL 5 ng/mL 5 µL Plasma, Serum 1h 15m

α-1-Acid Glycoprotein Rat ELISA

41-AGPRT-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 20m

α-1-Antitrypsin Canine ELISA

41-A1ACA-E01 12.5–800 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 15m

α-1-Antitrypsin Clearance ELISA

30-6752 3.3–90 µg/L 1.8 mg/dL 10 µL, 100 mg Plasma, Serum, Stool 2h 10m

α-1-Antitrypsin Mouse ELISA

41-A1AMS-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Serum 2h 5m

α-Fodrin Check IgG/IgA ELISA

35-AFCHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

AAI RIA 72-AAI See Protocol See Protocol 50 µL Plasma, Serum 2h 30m

ACE Kinetic High Sensitive Assay (CSF Samples)

01-KK-ACF 1–30 U/L 1 U/L 80 µL Cerebrospinal Fluid See Protocol

Active Dephosphorylated ß-Catenin ELISA

(S33/S37/T41)62-CATHU-E01 See Protocol See Protocol 100 µL

Cell Culture, Tissue Culture

4h 30m

AGP Canine ELISA 41-AGPCA-E016.17–1,500 ng/mL


AKT1/2 (pS473/pS474) and GSK3α/ß (pS21/pS9) ELISA

62-AGGHU-E01 See Protocol See Protocol 100 µLCell Culture, Tissue Culture

4h 35m

AKT1/2 ELISA (pS473/pS474)

62-AKTHU-E01 See Protocol See Protocol 100 µLCell Culture, Tissue Culture

4h 30m

Alpha-Lactalbumin IgA ELISA



Alpha-Lactalbumin IgG ELISA



ANA-8Pro ELISA 35-ANAHU-E01Cut-off: See Protocol


ANA-8S IgG ELISA 35-AN8HU-E01Cut-off: See Protocol


Annexin V ELISA 61-ANNHU-E01 0.78–50 ng/mL 0.33 ng/mL 50 µL Cell Culture, Serum 3h 10m

Anti-ANCA IgG Screening ELISA

13-CP-111 * Cut-off: See Protocol

See Protocol 25 µL Serum 30m

Anti-Annexin V ELISA 61-AANHU-E01 6.3–400 ng/mL 1.2 ng/mL 50 µL Cell Culture, Serum 2h 10m

Anti-ASCA IgA ELISA 27-GD79Cut-off: See Protocol


Anti-BPI IgA ELISA 27-GD11 See Protocol See Protocol 10 µL Plasma, Serum 1h 40m

Anti-BPI IgG ELISA 27-GD05 3.1–100 U/mL 3.1 U/mL 10 µL Plasma, Serum 1h 40m

Immunology

66



Anti-C1q Autoantibodies ELISA

01-EK-AC1QA 5–400 U/mL 1 U/mL 10 µL Plasma, Serum 2h 15m

Anti-Cardiolipin IgA/IgG/IgM Screen ELISA

27-GD28 20–200 U/mL 1.9 U/mL 10 µL Plasma, Serum 1h 10m

Anti-Cardiolipin IgG ELISA 27-GD26 20–200 U/mL 0.63 U/mL 10 µL Plasma, Serum 1h 10m

Anti-Cardiolipin IgM ELISA 27-GD27 8–100 U/mL 0.2 U/mL 10 µL Plasma, Serum 1h 10m

Anti-Cardiolipin-A ELISA 35-CAAHU-E01 3–300 APL/mL 1 APl/mL 10 µL Serum 1h



Anti-Casein IgG ELISA 27-GD43Cut-off: See Protocol


Anti-CaSR lgG ELISA 43-CASHU-E01 10–270 U/mL 2.5 U/mL 10 µL Plasma, Serum 1h 50m

Anti-Cathepsin G ELISA 35-CATHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-CENP-B IgG ELISA 35-CENHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-Cytomegalovirus IgG ELISA



Anti-Cytomegalovirus IgM ELISA



Anti-ds-DNA ELISA 27-GD10 12–200 IU/mL 1 IU/mL 10 µL Plasma, Serum, Urine 1h

Anti-ds-DNA IgA/IgG/IgM Screen ELISA

35-DSSHU-E01 3–300 U/mL 1 U/mL 10 µL Plasma, Serum, Urine 1h

Anti-ds-DNA-A ELISA 35-DSAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-ds-DNA-G ELISA 35-DSGHU-E01 3–300 IU/mL 1 IU/mL 10 µL Serum 1h

Anti-ds-DNA-M ELISA 35-DSMHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-Elastase ELISA 35-ELAHU-E01 3–300 U/mL 1 U/mL 100 µL Serum 1h

Anti-Epidermal Transglutaminase IgA ELISA



Anti-GBM & ANCA Rapid Screen ELISA

13-GCP-100 * See Protocol See Protocol 25 µL Serum 30m

Anti-GBM IgG ELISA 13-GP-104x * 10–320 U/mL See Protocol 5 µL Serum 2h




Anti-HAV ELISA 22-E10 10–50 mIU/mL 0.1 IU/mL 10 µL Plasma, Serum 4h

67



Anti-HAV IgG ELISA 68-4AGE3 See Protocol See Protocol 10 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

1h 30m

Anti-HAV IgM ELISA 68-4AME3 See Protocol See Protocol 5 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

2h

Anti-HBc IgG ELISA 68-4CBE3 See Protocol See Protocol 5 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

1h 30m

Anti-HBc IgM ELISA 68-4CME3 See Protocol See Protocol 5 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

2h 30m

Anti-HBs ELISA 68-4SBE3 See Protocol See Protocol 50 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

1h 30m

Anti-HCV ELISA 68-4NAE3 See Protocol See Protocol 100 µL Plasma, Serum 1h 45m












Anti-Helicobactor Pylori ELISA


See Protocol 100 mg Stool 3h

Anti-Herpes-1/2 IgM ELISA 27-GD88Cut-off: See Protocol


Anti-Histone C IgG ELISA 35-HSCHU-E01 3–300 U/mL 1 U/mL 10 µL Plasma, Serum 1h

Anti-Histone H1 IgG ELISA 35-HH1HU-E01 3–300 U/mL 1 U/mL 10 µL Plasma, Serum 1h 20m

Anti-Histone H2A IgG ELISA

35-H2AHU-E01 3–300 U/mL 1 U/mL 10 µL Plasma, Serum 1h

Anti-Histone H3 IgG ELISA 35-HH3HU-E01 3–300 U/mL 1 U/mL 10 µL Plasma, Serum 1h

Anti-Histone H4 IgG ELISA 35-HH4HU-E01 3–300 U/mL 1 U/mL 10 µL Plasma, Serum 1h

Anti-IFN ß Binding Antibodies ELISA

01-EK-IFNB20–500 titer units


Anti-Inositol-GM ELISA 35-INOHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-Insulin IgG ELISA 35-INAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-Intrinsic Factor IgG ELISA



Anti-Intrinsic Factor IgG ELISA (Recombinant)



Immunology

68



Anti-Jo-1 DIASTAT® ELISA 13-FAJO200 * See Protocol See Protocol 100 µL Plasma, Serum 2h

Anti-Jo-1 ELISA 35-JO1HU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h 30m

Anti-LC1 IgG ELISA 35-LC1HU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h 30m

Anti-LKM-1 IgG ELISA 35-LKMHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h 5m

Anti-M2 IgG ELISA 35-M2GHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-MAG Autoantibodies ELISA

01-EK-MAG ^ 1,000–70,000 titer units


Anti-MPO ELISA 27-GD02 3.1–100 U/mL 3.1 u/mL 20 µL Serum 1h 10m

Anti-MPO-ANCA IgG Capture ELISA

13-CAP-MPO-110 * 10–320 U See Protocol 5 µL Serum 2h





Anti-Ovarian Antibody ELISA

20-OVAHU-E01 6–100 U/mL 6 U/mL 5 µL Serum 2h 30m

Anti-Phosphatidyl-Serine-IgG/IgM ELISA

35-SERHU-E01 3–300 U/mL 1 U/mL 10 µL Plasma, Serum 1h

Anti-Phospholipid-8 IgG/IgM ELISA

35-P8PHU-E01Cut-off: See Protocol


Anti-Phospholipid-8Pro-A ELISA

35-8PAHU-E01Cut-off: See Protocol


Anti-Phospholipid-8S-A ELISA

35-8SAHU-E01Cut-off: See Protocol


Anti-PR3 ANCA ELISA 27-GD03 6.3–100 U/mL 0.5 U/mL 100 µL Plasma, Serum 1h

Anti-Prothrombin Factor II IgG ELISA

27-GD76 10–200 U/mL 10 U/mL 10 µL Plasma 1h 10m

Anti-Prothrombin IgA ELISA

35-PTAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-Prothrombin IgM ELISA

35-PTMHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-RF IgA ELISA 27-GD24 25–400 U/mL 3 U/mL 10 µL Plasma, Serum 1h

Anti-RF IgG ELISA 27-GD37 40–400 U/mL 3 U/mL 10 µL Plasma, Serum 1h

Anti-RF IgM ELISA 27-GD23 25–400 U/mL 3 U/mL 10 µL Plasma, Serum 1h

Anti-RF Screen ELISA 27-GD06 25–500 U/mL 3 U/mL 10 µL Plasma, Serum 1h 10m

Anti-Ribosomal P IgG ELISA 35-RIBHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-Rubella IgG ELISA 27-GD82Cut-off: See Protocol


69



Anti-Scl 70 IgG ELISA 35-SCIHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-SGPG Autoantibodies ELISA

01-EK-SGPG ^ Cut-off: See Protocol


Anti-SLA IgG ELISA 35-SLAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h 35m

Anti-Sm IgG ELISA 35-SMIHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-Sm/RNP-C IgG ELISA 35-SNRHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h



Anti-Soya IgG ELISA 27-GD45Cut-off: See Protocol


Anti-Sperm Antibody ELISA 20-ASAHU-E01 62–500 U/mL 62 U/mL 10 µL Plasma, Serum 1h 30m

Anti-ß 2 Glycoprotein IgA ELISA

27-GD30 See Protocol See Protocol 10 µL Plasma, Serum 1h 10m

Anti-ß 2 Glycoprotein IgG ELISA

27-GD31 5–100 U/mL 5 U/mL 10 µL Plasma, Serum 1h 10m

Anti-ß 2 Glycoprotein IgM ELISA



Anti-ß 2 Glycoprotein Screen ELISA

35-B2SHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-ß Lactoglobulin IgA ELISA



Anti-ß Lactoglobulin IgG ELISA



Anti-SS-A/Ro IgG ELISA 35-SSAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-SS-A-52 IgG ELISA 35-S52HU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-SS-A-60 IgG ELISA 35-S60HU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-SS-B-La IgG ELISA 35-SSBHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-ss-DNA ELISA 27-GD12 50–400 U/mL 1.1 U/mL 10 µL Plasma, Serum 50m

Anti-ss-DNA IgA ELISA 35-SDAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-ss-DNA IgA/IgG/IgM Screen ELISA

35-SSSHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-ss-DNA IgG ELISA 35-SDGHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-ss-DNA IgM ELISA 35-SDMHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Antithrombin IgA ELISA 35-TBAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Antithrombin IgG/IgM ELISA

35-TBGHU-E01 3–300 U/mL 1 U/mL 100 µL Serum 1h

Immunology

70



Anti-Tissue Transglutaminase IgA ELISA

27-GD70 5–100 U/mL 5 U/mL 10 µL Plasma, Serum 1h 10m









Against Remicade®)30-9650


See Protocol 100 µL Plasma, Serum Overnight

Anti-tTg-A ELISA 35-TGAHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h 30m

Anti-tTg-G ELISA 35-TGGHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-U1-70 ELISA 35-U17HU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-α Fodrin IgA ELISA 35-AFOHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Anti-α Fodrin IgG ELISA 35-AFGHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

APRIL ELISA 30-941115.6–1,000 ng/mL

15.6 ng/mL 100 µL Cell Culture, Plasma 6h 20m

APRIL ELISA 61-APRHU-E01 0.78–50 ng/mL 0.4 ng/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

ASCA IgG ELISA 27-GD78Cut-off: See Protocol


Aspergillus IgG Antibody ELISA


BAFF ELISA 30-9410 2–128 ng/mL 0.95 ng/mL 50 µL Cell Culture, Serum 5h 50 m

Bcl-2 ELISA 61-BCLHU-E01 0.50–32 ng/mL 0.5 ng/mL 20 µLPlasma, Serum, Tissue Culture

3h 10m

Blastocystis Antigen ELISA 50-714-01Cut-off: See Protocol

See Protocol See Protocol Stool 2h 15m

Bordetella Pertussis IgG ELISA

20-BPGHU-E01 * See Protocol See Protocol 10 µL Serum 1h 45m

C. Trachomatis IFA 50-580-01 See Protocol See Protocol 10 µL Serum 1h

C4a RIA 51-RK-519 40–1,300 ng/mL 40 ng/mL 450 µL Plasma 1h

C5a ELISA 20-C5AHU-E01 0.1–10 µg/L 0.02 µg/L 50 µL Plasma 35m

Calprotectin CALcheck Blue™ Dipstick (25 tests)

01-DS-CAL25Cut-off: See Protocol

See Protocol See Protocol Stool 10m

Calprotectin ELISA 01-EK-CAL ^ † 10–600 µg/g or 30–1,800 µg/g


71

* FDA Registered for In Vitro Diagnostic Use ^ Health Canada Licensed † Pending FDA Clearance

Products are for Research Use Only unless otherwise noted


Calprotectin ELISA (2x96 Wells)

01-EK-CAL-02 ^ 10–600 µg/g or 30–1,800 µg/g


Calprotectin Quantum Blue® High Range

Rapid Test01-LF-CHR25 ^ 100–1,800 µg/g 100 µg/g 80 mg Stool 15m

Calprotectin Quantum Blue® Rapid Test

01-LF-CAL25 ^ 30–300 µg/g 10 µg/g 80 mg Stool 12m

Cancer Antigen 125 ELISA 25-125HU-E01 15–400 U/mL 15 U/mL 100 µL Serum 1h 50m

Cancer Antigen 15-3 ELISA 25-153HU-E01 15–240 U/mL 5 U/mL 20 µL Serum 3h 20m

Cancer Antigen 19-9 ELISA 25-199HU-E01 15–240 U/mL 5 U/mL 100 µL Serum 3h 20m

Cancer Antigen 50 RIA 72-OCFM7-CA50 0.4–180 U/mL 0.4 U/mL 50 µL Plasma, Serum 4h

CCPlus® ImmunoscanMeasurement of anti-cyclic citrullinated peptide (CCP)

antibodies has become the method of choice for accurate

diagnosis of rheumatoid arthritis (RA). A diagnostic value

for the measurement of anti-CCP antibodies has been

found in relation to joint involvement and radiological

damage on early RA. Anti-CCP antibodies have a high

positive predictive value and can be detected years before

the development of clinical symptoms.1-6

The FDA registered CCPlus® Immunoscan assay provides

sensitive and semi-quantitative determination of IgG

antibodies to CCP in human sera. The assay is used to

detect antibodies in a single serum specimen and is as

sensitive as the rheumatoid factor and CCP3 tests but with

higher clinical specificity.6

Total # Patients(RA/Controls)

StratifiedSpecificity (%)

CCPlus®

Immunoscan CCP3 MCV RF

100/102 98.5% 73 67 62 17

Sensitivity at stratified specificity (%) for available RA diagnostic markers6

Immunology

72



Cancer Antigen 72-4 LIA 20-724HU-L01 0.14–100 U/mL 0.14 I/mL 10 µL Plasma, Serum 1h 30m

Cardiolipin IgG/IgM ELISA 35-CAGHU-E01 3–300 GPL/mL 1 GPl/mL 10 µL Serum 1h

Caspase-8 ELISA 61-CA8HU-E01 0.16–10 ng/mL 0.10 ng/mL 50 µLCell Culture, Serum, Tissue Culture

3h

Caspase-9 ELISA 61-CA9HU-E01 1.6–100 ng/mL 0.4 ng/mL 50 µLCell Culture, Serum, Tissue Culture

3h 10m

Cathepsin L ELISA 61-CALHU-E01 3.1–50 ng/mL 1.7 ng/mL 50 µL Cell Culture, Serum 3h 10m

CCPlus Immunoscan® (Anti-CCP ELISA)

13-RA-96PLUS * 25–3,200 U/mL See Protocol 10 µL Serum 2h

CD153 ELISA 61-153HU-E01 1.6–100 ng/mL 0.5 ng/mL 50 µL Cell Culture, Serum 3h 10m

CEA ELISA 25-CEAHU-E01 3–120 ng/mL 3 ng/mL 50 µL Serum 1h 20m

1 Rantapää-Dahlqvist S, de Jong BA, Berglin E, et al. Antibodies against cyclic

citrullinated peptide and IgA rheumatoid factor predict the development of

rheumatoid arthritis. Arthritis Rheum. Oct 2003; Vol 48 No 10: 2741-2749.

2 van Venrooij WJ, van Beers JJ, Pruijn GJ. Anti-CCP antibodies: the past, the

present and the future. Nat Rev Rheumatol. Jun 2011; Vol 7 No 7: 391-398

3 Svärd A, Kastbom A, Söderlin MK, et al. A comparison between IgG- and

IgA-class antibodies to cyclic citrullinated peptides and to modified

citrullinated vimentin in early rheumatoid arthritis and very early arthritis. J

Rheumatology. Jul 2011; Vol 38 No 7: 1265-1272.

4 Aletaha D, Neogi T, Silman AJ, Et al. 2010 Rheumatiod arthritis classification

criteria: a American College of Rheumatology/European League Against

Rheumatism collaborative initiative. Arthritis Rheum. Sep 2010; Vol 69 No

9: 1580-1588.

5 Pruijn GJ, Wiik A, van Venrooij WJ. The use of citrullinated peptides and

proteins for the diagnosis of rheumatoid arthritis. Arthritis Res Ther. 2010;

Vol 12 No 1: 203.

6 Bizzaro N, Tonutti E, Tozzoli R, Villalta D. Analytical and diagnostic

characteristics of 11 2nd- and 3rd- generation immunoenzymatic methods

for the detection of antibodies to citrullinated proteins. Clin Chem. Aug

2007; Vol 53 No 8: 1527-1533.

View CCPlus® Immunoscan assay 13-RA-96PLUS on page 73

73



CeliCheck ELISA (Tissue Transglutaminase

IgA/IgG ELISA)35-CELHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h 30m

Cell Proliferation & Toxicity Assay (EZ4U Assay)

04-BI-5000 See Protocol See Protocol 200 µL Cell Culture 2h

Chemerin ELISA 22-CHM-E102 25–600 pg/mL 0.005 pg/mL 10 µL Plasma, Serum 2h 30m

Chlamydia IgA IFA 50-513-01 See Protocol See Protocol 10 µL Serum 1h

Chlamydia IgG IFA 50-511-01 See Protocol See Protocol 10 µL Serum 1h

Chlamydia IgM IFA 50-512-01 See Protocol See Protocol 5 µL Serum 1h

Chymotrypsin ELISA 30-6910 20–1,000 ng/mL 20 ng/mL 100 mg Stool 2h 30m

CIC ELISA (C1q) 01-EK-CIC * 1–50 µg Eq/mL 0.6 µg Eq/mL 20 µL Serum 2h

CINC-1 Rat ELISA 48-CINRT-E01 12.5–800 pg/mL 12.5 pg/mL 25 µLEDTA Plasma, Heparin Plasma, Serum

2h 30m

Complement Factor 3 Canine ELISA

41-CO3CA-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h

Complement Factor 3 Guinea Pig ELISA

41-CO3GP-E013.125–200 ng/mL


Complement Factor 3 Mouse ELISA

41-CO3MS-E013.125–200 ng/mL


Complement Factor 3 Rat ELISA

41-CO3RT-E01 12.5–800 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 50m

Complement Functional Screen Total ELISA

13-COMPL-300Cut-off: See Protocol


Complement System Alternative Pathway ELISA

13-COMPL-AP330Cut-off: See Protocol


C-Reactive ProteinCRP is released as a part of the acute-phase innate system

response which aims to neutralize the inflammatory agent

and to promote the healing of the injured tissue. During the

acute-phase response, CRP levels can increase by up to a

1000-fold and levels remain high as long as the damage

persists. Because the half-life of this marker is constant, CRP

levels can be used to study the severity of the precipitating

cause of inflammation. ALPCO has immunoassays available

for testing CRP levels in plasma and serum from canine,

equine, feline, human, mouse, porcine, rabbit and rat.

View CRP assays on page 75

Immunology

74



Complement System Classical Pathway ELISA

13-COMPL-CP310Cut-off: See Protocol


Complement System MBL Pathway ELISA

13-COMPL-MP320Cut-off: See Protocol


CPA IgA ELISA 27-GD121 6.25–100 µmol/L 6.25 U/mL 10 µL Plasma, Serum 1h

CPA IgG ELISA 27-GD110 6.25–100 µmol/L 6.25 U/mL 10 µg Plasma, Serum 1h

CPA IgM ELISA 27-GD120 0–100 U/mL 6.25 U/mL 10 µL Plasma, Serum 1h

C-Reactive Protein Porcine ELISA

41-CRPPO-E01 6.25–200 ng/mL 5 ng/mL 5 µL Serum 25m

CREB ELISA (pS133) 62-CREHU-E01 See Protocol See Protocol 100 µLCell Culture, Tissue Culture

4h 30m

Cripto-1 ELISA 20-CRIHU-E01 50–800 pg/mL 8.4 pg/mL 50 µL Serum 3h 50m

Crohns Check ELISA (Anti-Saccharomyces

Cerevisiae IgA/IgG ELISA)35-CROHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

CRP Canine ELISA 41-CRPCA-E012.125–200 ng/mL

5 ng/mL 5 µL Plasma, Serum 25m

CRP ELISA 30-9710s 1.9–150 ng/mL 0.124 ng/mL 10 µLPlasma, Serum, Stool, Urine

2h 15m

CRP Equine ELISA 41-CRPEQ-E01 10–320 ng/mL 10 ng/mL 5 µL Plasma, Serum 1h 15m

CRP Feline ELISA 41-CRPFE-E013.125–200 ng/mL


CRP Mouse ELISA 41-CRPMS-E01 0.39–25 ng/mL 0.39 ng/mL 5 µL Plasma, Serum 25m

CRP Rabbit ELISA 41-CRPRB-E01 6.25–200 ng/mL 5 ng/mL 2 µL Plasma, Serum 25m

CRP Rat ELISA 41-CRPRT-E01 6.25–200 ng/mL 5 ng/mL 5 µL Plasma, Serum 25m

CTGF ELISA 20-CTGHU-E01 1–500 ng/mL 3.24 ng/mL 25 µL Serum 3h 45m

Cu/ZnSOD ELISA 61-CUZHU-E01 0.08–5 ng/mL 0.04 ng/mL 10 µL

Amniotic Fluids, Cell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

1h 10m

CYFRA LIA (21-1) 20-CYFHU-L01 3–50 ng/mL 0.03 ng/mL 50 µL Heparin Plasma, Serum 30m

Cytochrome C ELISA 61-CYCHU-E01 0.08–5 ng/mL 0.05 ng/mL 100 µL Tissue Culture 3h 10m

Dengue NS1 ELISA 65-DENHU-E01 See Protocol See Protocol 50 µL Serum 1h 51m

DIASTAT® ANA ELISA 13-FANA200 * See Protocol See Protocol 10 µL EDTA Plasma, Serum 2h

DIASTAT® MPO ANCA ELISA

13-FMPO200 * 0–100 U/mL See Protocol 100 µL Plasma, Serum 2h

75



EGF ELISA 45-EGFHU-E01 3.9–250 pg/mL 1 pg/mL 100 µLCell Culture, Citrate Plasma, Heparin Plasma, Serum, Urine

4h

EGF Receptor ELISA 30-7110 3–24 fmol/mL 0.4 fmol/mL 200 µL Tissue Culture Overnight

ENA-6Pro ELISA 35-E6PHU-E01Cut-off: See Protocol


ENA-6S ELISA 35-E6SHU-E01 10–100 U/mL 1 U/mL 10 µL Serum 1h

Eotaxin Mouse ELISA 61-EOTMS-E0131.3–2,000 pg/mL


ERK1/2 ELISA (pT202/Y204/pT185/Y187)

62-ERKHU-E01 See Protocol See Protocol 100 µLCell Culture, Tissue Culture

4h 35m

FGF-21 ELISA 62-FGFHU-E01 30–1,920 pg/mL 10.4 pg/mL 100 µLCell Culture, Plasma, Serum

2h 35m

FGF-21 Mouse ELISA 62-FGFMS-E0131.2–2,000 pg/mL


2h 35m

GAD ELISA 21-GADHU-E01 0.6–2.99 U/mL See Protocol 10 µL Serum 2h 30m

Galectin-3 ELISA 61-GALHU-E01 0.39–25 ng/mL 0.12 ng/mL 50 µLCell Culture, EDTA Plasma, Serum

3h 10m

GanglioCombi® Anti-Ganglioside Auto

Antibody ELISA01-EK-GCO



GanglioCombi® Light Anti-Ganglioside ELISA

01-EK-GCL-GMCut-off: See Protocol


G-CSF ELISA 45-GCSHU-E01 39–2,500 pg/mL 20 pg/mL 50 µLCell Culture, EDTA Plasma, Serum

3h

GM-CSF ELISA 61-GMCHU-E01 7.8–500 pg/mL 2.9 pg/mL 50 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

3h 10m

GM-CSF Mouse ELISA 61-GMCMS-E01 3.9–250 pg/mL 2 pg/mL 50 µL Cell Culture, Serum 3h

GM-CSF Rat ELISA 61-GMCRT-E01 7.8–500 pg/mL 3.5 pg/mL 50 µL Cell Culture, Serum 3h 10m

Granzyme A ELISA 61-GRAHU-E01 0.8–600 pg/mL 0.4 pg/mL 50 µLCell Culture, Plasma, Serum

2h

Granzyme B ELISA 61-GRBHU-E01 0.66–480 pg/mL 0.2 pg/mL 50 µL Cell Culture, Serum 2h

GSK3α/ß (pS21/pS9) and Active ß-Catenin ELISA

62-GCAHU-E01 See Protocol See Protocol 100 µLCell Culture, Tissue Culture

4h 30m

GSK3α/ß ELISA (pS21/pS9) 62-GSKHU-E01 See Protocol See Protocol 100 µLCell Culture, Tissue Culture

4h 30m

HAHA ELISA 43-HAHHU-E01 1–27 µg/mL 1 µg/mL 25 µL Plasma, Serum 1h 50m

HAMA ELISA 43-HAMHU-E01 40–1,500 ng/mL 2 ng/mL 25 µL Serum 1h 50m



Immunology

76













HBe/Anti-HBe ELISA 68-4BNE3 See Protocol See Protocol 50 µL Plasma, Serum 2h 30m

HBsAg ELISA 68-4SGE3 See Protocol See Protocol 50 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Plasma, Serum

1h 50m

hCOMP ELISA 13-COMP-200 10–80 ng/mL 10 ng/mL 5 µL Serum Overnight

HER-2 ELISA 61-HERHU-E01 0.16–10 ng/mL 0.06 ng/mL 100 µLAmniotic Fluids, Cell Culture, EDTA Plasma, Heparin Plasma, Serum

2h 10m


20-HSMHU-E01 See Protocol See Protocol 10 µL Serum 1h 45m

Histamine ELISA 17-HISHU-E01 0.3–30 ng/mL See Protocol 25 µL Plasma, Urine Overnight

Histamine ELISA (Cell Culture)

17-HISCC-E01 0.5–50 ng/mL 0.2 ng/mL 20 µL Cell Culture, Urine 1h

Histamine Research ELISA 17-HISRT-E01-RES 0.3–30 ng/mL 0.2 ng/mL 10 µL EDTA Plasma Overnight

IFN-gamma ELISA 61-IFGHU-E01 1.6–100 pg/mL 0.99 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

IFN-gamma Monkey ELISA 45-IFNMK-E0115.6–1,000 pg/mL


4h

IFN-gamma Mouse ELISA 61-IFGMS-E0115.6–1,000 pg/mL


IFN-gamma Rat ELISA 61-IFGRT-E0131.3–2,000 pg/mL


IgA Chicken ELISA 41-IGACH-E01 12.5–400 ng/mL 12.5 ng/mL 100 µL Plasma, Serum 50m

IgA ELISA 41-IGAHU-E01 12.5–800 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 10m

IgA Mouse ELISA 41-IGAMS-E0112.5–3,025 ng/mL


77



IgA Rat ELISA 41-IGART-E0131.25–1,000 pg/mL


IgD ELISA 41-IGDHU-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 30m

IgE Canine ELISA 41-IGECA-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

IgE Equine ELISA 41-IGEEQ-E013.125–100 ng/mL


IgE Feline ELISA 41-IGEFE-E01 12.5–400 ng/mL 12.5 ng/mL 10 µL Plasma, Serum 2h 15m

IgE Mouse ELISA 41-IGEMS-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

IgE Rat ELISA 41-IGERT-E01 1–32 ng/mL 1 ng/mL 10 µL Plasma, Serum 2h 15m

IgE Total ELISA 25-IGEHU-E01 * 10–800 IU/mL 5 IU/mL 20 µL Serum 1h 20m

IgG Canine ELISA 41-IGGCA-E01 25–800 ng/mL 25 ng/mL 5 µL Plasma, Serum 45m

IgG Mouse ELISA 41-IGGMS-E019.375–600 ng/mL


IgG Rat ELISA 41-IGGRT-E01 25–800 ng/mL 25 ng/mL 5 µL Plasma, Serum 1h 45m

IgG Total ELISA 30-6510 0.32–5 mg/L 0.14 mg/L 10 µLCell Culture, Plasma, Serum

2h 5m

IgG1 Mouse ELISA 41-IG1MS-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 30m

IgG2A Mouse ELISA 41-I2AMS-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 30m

IgG2B Mouse ELISA 41-I2BMS-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

IgG3 Mouse ELISA 41-IG3MS-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 30m

IgM Canine ELISA 41-IGMCA-E01 25–800 ng/mL 25 ng/mL 5 µL Plasma, Serum 1h 15m

IgM Chicken ELISA 41-IGMCH-E013.125–200 ng/mL


IgM ELISA 41-IGMHU-E01 7.8–500 ng/mL 7.8 ng/mL 5 µL Plasma, Serum 1h 45m

IgM Mouse ELISA 41-IGMMS-E01 25–800 ng/mL 25 ng/mL 5 µL Plasma, Serum 1h 45m

IgM Rat ELISA 41-IGMRT-E0131.25–1,000 ng/mL


IgY Chicken ELISA 41-IGYCH-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

IL-10 ELISA 61-I10HU-E01 3.15–200 pg/mL 1 pg/mL 50 µLCell Culture, Citrate Plasma, Heparin Plasma, Serum

3h 10m

IL-10 Monkey ELISA 61-I10MK-E01 3.9–250 U/mL 1.3 U/mL 50 µL Cell Culture, Serum 3h 10m

IL-10 Mouse ELISA 61-I10MS-E0115.6–1,000 pg/mL


4h 10m

Immunology

78



IL-10 Porcine ELISA 45-I10PO-E01 7.8–500 pg/mL 3 pg/mL 50 µLCell Culture, Plasma, Serum

3h

IL-10 Rat ELISA 61-I10RT-E0115.6–1,000 pg/mL


IL-10 Ultrasensitive ELISA 45-I10HUU-E01 0.78–50 pg/mL 0.2 pg/mL 100 µLCell Culture, Plasma, Serum

4h

IL-10 Ultrasensitive ELISA 45-I10HUU-E05 0.78–50 pg/mL 0.2 pg/mL 100 µLCell Culture, Plasma, Serum

4h

IL-12 ELISA (p70) 61-P12HU-E01 3.1–200 pg/mL 2.1 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

IL-12 Monkey ELISA (p70) 61-P12MK-E01 3.9–250 U/mL 2.6 U/mL 50 µL Cell Culture, Serum 3h 10m

IL-12 Mouse ELISA (p70) 61-P12MS-E0115.6–1,000 pg/mL


IL-12/IL-23 ELISA (p40) 61-ILPHU-E0131.3–2,000 pg/mL

< 10 pg/mL 50 µLCell Culture, Citrate Plasma, Heparin Plasma, Serum

4h 10m

IL-13 ELISA 61-I13HU-E01 1.6–100 pg/mL 0.7 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

2h 10m

IL-13 Mouse ELISA 61-I13MS-E01 7.8–500 pg/mL 2.8 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Serum

3h 10m

IL-15/IL-15R Mouse ELISA 61-I15MS-E01 6.3–400 pg/mL 2 pg/mL 50 µLCell Culture, EDTA Plasma, Serum

3h 30m

IL-17A ELISA 61-ILAHU-E01 1.6–100 pg/mL 0.5 pg/mL 50 µLCell Culture, Citrate Plasma, Heparin Plasma, Serum

3h 10m

IL-17A Mouse ELISA 61-ILAMS-E01 7.8–500 pg/mL 1.6 pg/mL 50 µL Cell Culture, Serum 3h 10m

IL-17A Rat ELISA 61-ILART-E01 1.6–100 pg/mL 1 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 30m

IL-17AF ELISA 61-IAFHU-E0131.3–2,000 pg/mL

8.8 pg/mL 50 µLCell Culture, Citrate Plasma, Heparin Plasma, Serum

3h 30m

IL-17AF Mouse ELISA 61-IAFMS-E01 3.9–250 pg/mL 3.3 pg/mL 50 µLCell Culture, EDTA Plasma, Serum

3h 30m

IL-17F ELISA 61-ILFHU-E0131.3–2,000 pg/mL

15.5 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 30m

IL-17F Mouse ELISA 61-ILFMS-E0162.5–4,000 pg/mL


4h 30m

IL-18 Porcine ELISA 61-I18PO-E0139.1–2,500 pg/mL


79



IL-1RA ELISA 61-1RAHU-E01109–7,000 pg/mL

30 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 30m

IL-1ß Rat ELISA 61-I1BRT-E0131.3–2,000 pg/mL


IL-1α ELISA 61-I1AHU-E01 1.6–100 pg/mL 1.1 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

IL-1α Mouse ELISA 61-I1AMS-E01 7.8–500 pg/mL 4 pg/mL 50 µL Cell Culture, Serum 3h 10m

IL-1α Rat ELISA 61-I1ART-E0115.6–1,000 pg/mL



9.9 pg/mL 100 µL


3h 10m

IL-2 Monkey ELISA 61-IL2MK-E01 20–1,250 U/mL 10 U/mL 100 µL Cell Culture, Serum 3h 10m



IL-2 Rat ELISA 61-IL2RT-E0162.5–2,000 pg/mL


IL-21 ELISA 61-I21HU-E01 78–5,000 pg/mL 20 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Serum

4h 10m

IL-22 ELISA 61-I22HU-E0131.3–2,000 pg/mL


4h 10m

IL-22 Mouse ELISA 61-I22MS-E01 7.8–500 pg/mL 5 pg/mL 50 µLCell Culture, Citrate Plasma, Serum

3h 30m



3h

IL-23 Mouse ELISA 61-I23MS-E0131.3–2,000 pg/mL

16 pg/mL 50 µLCell Culture, Citrate Plasma, Serum

4h 10m



3h 30m

IL-29 ELISA (IFN-lambda1) 61-IFLHU-E0115.6–1,000 pg/mL


3h 30m

IL-3 ELISA 45-IL3HU-E01 7.8–500 pg/mL 1 pg/mL 100 µLCell Culture, Citrate Plasma, EDTA Plasma, Plasma, Serum

4h

IL-31 ELISA 61-I31HU-E01 7.8–500 pg/mL 1 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

4h 30m

Immunology

80



IL-33 ELISA 61-I33HU-E01 7.8–500 pg/mL 0.2 pg/mL 50 µLCell Culture, Citrate Plasma, Heparin Plasma, Serum

4h 30m

IL-4 ELISA 61-IL4HU-E01 7.8–500 pg/mL 1.3 pg/mL 50 µLCell Culture, Plasma, Serum

3h 10m

IL-4 Mouse ELISA 61-IL4MS-E01 3.9–250 pg/mL 2 pg/mL 50 µL Cell Culture, Serum 3h 10m



IL-4 Rat ELISA 45-IL4RT-E01 7.8–500 pg/mL 2 pg/mL 100 µL Cell Culture, Serum 3h

IL-4 Rat ELISA 61-IL4RT-E01 1.6–100 pg/mL 0.2 pg/mL 50 µL Cell Culture, Serum 3h 10m

IL-5 ELISA 61-IL5HU-E01 7.8–500 pg/mL 1.5 pg/mL 50 µL Cell Culture, Serum 3h 10m

IL-5 Mouse ELISA 61-IL5MS-E01 7.8–500 pg/mL 3.3 pg/mL 50 µL Cell Culture, Serum 3h 10m


3h


3h




3h 30m



2 pg/mL 50 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

3h 10m

IL-8 Monkey ELISA 61-IL8MK-E01 19.5–1,250 U/mL 3 U/mL 50 µL Cell Culture, Serum 3h 10m



IL-8 Ultrasensitive ELISA 45-IL8HUU-E01 0.39–25 pg/mL 0.1 pg/mL 100 µLCell Culture, Plasma, Serum, Urine

4h

IL-9 ELISA 61-IL9HU-E01 3.1–200 pg/mL 0.5 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 30m

Insulin Autoantibody ELISA 21-IAAHU-E01Cut-off: See Protocol


IP-10 Mouse ELISA 61-IP1MS-E01 7.8–500 pg/mL 6.5 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Serum

3h 10m

Islet Cell Antibody ELISA 21-ICAHU-E01Cut-off: See Protocol


Japanese Encephalitis IgG ELISA

65-JEGHU-E01 See Protocol See Protocol 4 µL Serum 2h 16m

81



Japanese Encephalitis IgM ELISA

65-JEMHU-E01 See Protocol See Protocol 4 µL Serum 3h 16m

Kappa ELISA 41-KAPHU-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h

L1-CAM ELISA 20-LCAHU-E01 8.75–280 ng/mL 0.1 ng/mL 50 µL Serum 1h 45m

Lactoferrin ELISA 41-LACHU-E013.125–100 ng/mL

3.125 ng/mL 5 µL Milk, Plasma, Serum 1h 15m

Lambda ELISA 41-LAMHU-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h

L-Citrulline ELISA 30-6600 6.25–400 µM 6.25 µM 500 µL Plasma, Serum, Urine 1h 30m

Leukemia Inhbitory Factor ELISA

61-LIFHU-E01 3.15–200 pg/mL 0.7 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h

MCP-1 ELISA 61-MCPHU-E0115.6–1,000 pg/mL

2.3 pg/mL 20 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

2h 10m

MCP-1 Mouse ELISA 61-MCPMS-E0115.6–1,000 pg/mL


MCP-1 Rat ELISA 45-MCPRT-E01 11.7–750 pg/mL 8 pg/mL 50 µLCell Culture, Plasma, Serum

2h 30m

Measles IgG ELISA 20-MEGHU-E01 * See Protocol See Protocol 10 µL Serum 1h 45m

MMP-13 ELISA 61-M13HU-E01 0.31–20 ng/mL 0.18 ng/mL 50 µL Cell Culture, Serum 3h 10m

MMP-3 ELISA 48-MP3HU-E01 12.5–800 ng/mL 0.01 ng/mL 40 µL Serum 2h

MMP-9 ELISA 61-MP9HU-E01 0.23–15 ng/mL 0.05 ng/mL 10 µLCell Culture, Citrate Plasma, Heparin Plasma, Serum

3h 10m

MPO ELISA 30-6630 1.2–100 ng/mL 1.6 ng/mL 100 mg Stool, Urine 2h 20m

MPO ELISA (Serum and Plasma)

30-6631A 1.9–30 ng/mL 1.08 ng/mL 10 µL Plasma, Serum 3h 20m

MPO Mouse ELISA 41-MPOMS-E01 0.31–20 ng/mL 0.31 ng/mL 5 µL Plasma, Serum 1h 30m


Mycobacterium Tuberculosis IgG ELISA

65-TBGHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

Mycobacterium Tuberculosis IgM ELISA

65-TBMHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

Neopterin ELISA 14-HD-99.1 2–250 nmol/L 2 nmol/L 50 µL Plasma, Serum, Urine 2h 30m

Neopterin RIA 14-HD-97.1 3–729 nmol/L 0.8 nmol/L 20 µL Biological Fluids 1h


3h 10m

Immunology

82




3h 10m


3h 10m


3h 10m


3h 10m


1h

NGAL Rat ELISA 54-046 4–400 pg/mL 0.5 pg/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h 10m

Nucleo-h IgG ELISA 35-NUCHU-E01 3–300 U/mL 1 U/mL 10 µL Serum 1h

Orientia Tsutsugamushi IgG ELISA

65-OTGHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

Orientia Tsutsugamushi IgM ELISA

65-OTMHU-E01 See Protocol See Protocol 4 µL Serum 1h 16m

NeopterinNeopterin serves as a marker of activation of the adaptive

immune system. It is synthesized by macrophages

upon stimulation with the cytokine interferon-gamma

and is indicative of a pro-inflammatory immune status.

Measurement of circulating neopterin concentrations

provides information about cellular immune activation in

humans under the control of T helper cells type 1. ALPCO

offers both an ELISA and an RIA for the measurement of

Neopterin in plasma, serum and urine.Neopterin [nmol/l]

Op

tica

l Den

sity

(OD

)

2

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

1.6

1.8

20 200

Representative Standard Curve from 14-HD-99.1

View neopterin assays 14-HD-99.1 and 14-HD-97.1 on page 82

83


Immunology



Ovotransferrin Chicken ELISA

41-OVOCH-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h

p38 α ELISA (pT180/Y182) 62-P38HU-E01 See Protocol See Protocol 100 µLCell Culture, Tissue Culture

4h 30m

p53 ELISA 61-P53HU-E01 0.78–50 U/mL 0.33 U/mL 50 µL Cell Culture, Serum 3h 10m

PAI-1 ELISA 61-PAIHU-E01 78–5,000 pg/mL 29 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

PAP Complex ELISA 20-PCOHU-E02 50–5,000 µg/L 10 µg/L 50 µL Plasma 45m

PAPP-A ELISA 20-PAPHU-E01 1–30 µg/mL 0.133 µg/mL 10 µL Plasma, Serum 1h 15m

PAPP-A Ultrasensitive ELISA

55-PAPHUU-E0111.25–450 ng/mL

0.023 ng/mL 100 µL Serum 45m

Pertussis IgG ELISA 13-PERT-PTG2 * Cut-off: See Protocol

See Protocol See Protocol Serum 2h

Platelet Derived Growth Factor ELISA

61-PDGHU-E0131.3–2,000 pg/mL


3h 30m

PMN Elastase Ultrasensitive ELISA

30-6840 0.37–10 ng/mL 0.12 ng/mL 100 mg, 100 µLPlasma, Seminal Fluids, Serum, Stool

3h 15m

PMN-Elastase ELISA 61-PMNHU-E01 0.16–10 ng/mL 1.98 ng/mL 100 µLCell Culture, Plasma, Serum

2h

Porphyrins HPLC 30-2601 See Protocol See Protocol 1 mL Urine See Protocol

PR3-ANCA Capture ELISA 13-CAP-PR3 * Cut-off: See Protocol


Progranulin ELISA 22-PRG-E103 0.75–25 ng/mL 0.018 ng/mL 10 µL Plasma, Serum 2h

PSA NCAM ELISA 67-ABC0027B 0.25–16 ng/mL 0.25 ng/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

Overnight

PSA Total ELISA 25-PSAHU-E01 2–120 ng/mL 1 ng/mL 50 µL Serum 2h 20m

PSA Total LIA 11-PSAHU-L01 0.1–100 ng/mL 0.082 ng/mL 50 µL Serum 1h 30m

RANTES/CCL5 ELISA 45-RANHU-E0131.2–2,000 pg/mL


RC4d Antibody 04-BI-RC4D See Protocol See Protocol See Protocol See Protocol See Protocol

s90K/Mac-2BP ELISA 61-MACHU-E01 12.5–200 ng/mL 0.92 ng/mL 100 µLCell Culture, Plasma, Serum

1h

sAPO-1/Fas ELISA 61-APOHU-E0115.6–1,000 pg/mL


sCD134 ELISA (OX40) 61-134HU-E01 78–5,000 pg/mL 1.8 pg/mL 25 µL Cell Culture, Serum 3h 10m

sCD137 ELISA 61-137HU-E01 0.78–50 ng/mL 0.2 ng/mL 50 µL Cell Culture, Serum 3h 10m

Immunology

84



sCD152/CTLA-4 ELISA 61-CTLHU-E01 0.16–10 ng/mL 0.13 ng/mL 10 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

3h 10m

sCD23 ELISA 61-C23HU-E01 12.5–400 U/mL 6.8 U/mL 100 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

sCD26 ELISA 61-C26HU-E01 15.6–500 ng/mL 7.3 ng/mL 20 µL Cell Culture, Serum 4h 10m

sCD28 ELISA 61-C28HU-E01 0.78–50 ng/mL 0.18 ng/mL 50 µL Cell Culture, Serum 3h 10m

sCD30 ELISA 61-C30HU-E01 1.6–100 ng/mL 0.3 ng/mL 25 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

sCD40 ELISA 61-C40HU-E01 7.8–500 pg/mL 1.3 pg/mL 50 µLAmniotic Fluids, Cell Culture, EDTA Plasma, Heparin Plasma, Serum

3h 10m

sCD40L ELISA 61-40LHU-E01 0.16–10 ng/mL 0.06 ng/mL 20 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

2h 10m

sCD40L Mouse ELISA 61-40LMS-E01 0.31–20 ng/mL 0.14 ng/mL 50 µL Cell Culture, Serum 3h 10m

RC4dWithin the adaptive system’s cascade of events is the classical complement

pathway, consisting of a number of small proteins in the blood including,

but not limited to C1, C2, C3 and C4. Until recently, it was thought that

the humoral pathway was most often associated with an acute transplant

rejection. However, there are visible features of humoral rejection seen in

more chronic rejection settings, especially in patients with renal allografts,

one of which is the deposition of C4d in the capillary bed of renal allografts.

C4d is a split product of C4. Because C4d covalently binds to adjacent

membrane surfaces it can be easily detected using conventional methods. As such, immunostaining with an antibody

targeting C4d is an important method that can be used to identify humoral-graft rejection.1

1 Troxwell, ML et al. Comparison of C4d immunostaining methods in renal allograft biopsies. Clinical J American Society Nephrology. May 2006; Vol 1 No 3: 583-591.

View RC4d assay 04-BI-RC4D on page 84

Paraffin embedded tissue sections

85



sCD44std ELISA 61-C44HU-E01 0.13–4 ng/mL 0.02 ng/mL 20 µL


3h 10m

sCD44var ELISA (v5) 61-445HU-E01 0.31–10 ng/mL 0.14 ng/mL 20 µL


3h 10m

sCD44var ELISA (v6) 61-446HU-E01 0.32–10 ng/mL 0.05 ng/mL 20 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

sCD-8 ELISA 61-CD8HU-E01 31–2,000 U/mL 7 U/mL 50 µL Citrate Plasma, Serum 3h 10m

Sclero-Profile IgG ELISA 35-SCLHU-E01Cut-off: See Protocol


Secretory IgA ELISA 30-8870 22.2–600 ng/mL 13.4 ng/mL 100 mg, 10 µL Saliva, Stool 2h 10m



SeroMP lgG ELISA 50-FA261-01M 10–100 BU/mL See Protocol 10 µL Serum 2h 15m

SeroMP lgG ELISA (2 Plate) 50-B261-01M 10–100 BU/mL See Protocol 10 µL Serum 2h 15m

SeroMP lgM ELISA 50-FA262-01M 10–100 BU/mL See Protocol 10 µL Serum 2h 15m

SeroMP lgM ELISA (2 Plate) 50-B262-01M 10–100 BU/mL See Protocol 10 µL Serum 2h 15m



41-SAAMS-E0131.25–2,000 ng/mL


Serum Amyloid ß Auto-Antibody ELISA

20-SABHU-E01 1–120 RTU See Protocol 20 µL Serum 2h 30m

sE-selectin ELISA 61-ESEHU-E01 0.8–50 ng/mL 0.3 ng/mL 20 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

2h 10m

sE-selectin Monkey ELISA 61-ESEMK-E01 1.9–60 U/mL 0.4 U/mL 20 µL Cell Culture, Serum 2h 10m

sFas Ligand ELISA 61-FALHU-E01 0.16–10 ng/mL 0.07 ng/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

sICAM-1 ELISA 61-IC1HU-E01 6.25–100 ng/mL 2.2 ng/mL 10 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

1h 10m

sICAM-1 Monkey ELISA 61-IC1MK-E01 6.3–100 U/mL 1.1 U/mL 10 µL Cell Culture, Serum 1h 10m

Immunology

86



sICAM-1 Ultrasensitive ELISA

61-IC1HUU-E01 0.63–10 ng/mL 0.33 ng/mL 100 µL

Amniotic Fluids, Cell Culture, EDTA Plasma, Heparin Plasma, Plasma, Serum, Urine

2h 10m

sICAM-2 ELISA 61-IC2HU-E01 0.75–24 U/mL 0.30 U/mL 100 µL

Amniotic Fluids, Cell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine, Whole Blood

1h

sICAM-3 ELISA 61-IC3HU-E01 0.78–50 ng/mL 0.38 ng/mL 20 µL

Amniotic Fluids, Cell Culture, EDTA Plasma, Heparin Plasma, Serum, Urine

2h 10m

sIL-2R ELISA 61-I2RHU-E01 0.31–20 ng/mL 0.27 ng/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 10m

sIL-6R ELISA 61-I6RHU-E01 0.08–5 ng/mL 0.01 ng/mL 20 µL

Amniotic Fluids, Cell Culture, EDTA Plasma, Heparin Plasma, Serum, Urine

2h 10m

sL-selectin ELISA 61-LSEHU-E01 0.4–25 ng/mL 0.2 ng/mL 50 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

2h 10m

sPECAM-1 ELISA 61-PECHU-E01 0.47–30 ng/mL 0.06 ng/mL 10 µL

Cell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

3h 10m

sPECAM-1 Monkey ELISA 61-PECMK-E01 0.48–30 U/mL 0.06 U/mL 10 µL Cell Culture, Serum 3h 10m

sP-selectin ELISA 61-PSEHU-E01 0.63–40 ng/mL 0.20 ng/mL 10 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

2h 30m

sP-selectin Monkey ELISA 61-PSEMK-E01 0.8–50 U/mL 1.3 U/mL 10 µL Cell Culture, Serum 2h 30m

sPSGL-1 ELISA 61-PSGHU-E01 1.6–50 U/mL 1 U/mL 100 µL Cell Culture, Serum 3h 10m

ß Defensin 2 ELISA 30-6500 0.1–3 ng/mL 0.077 ng/mL 100 mg Stool 3h 30m

ß-4 Thymosin ELISA 30-9520 0.16–10 µg/mL 0.1 µg/mL 50 µL Serum, Tissue Culture 2h 20m

sTNF-R ELISA (60kDa) 61-TN6HU-E01 0.08–5 ng/mL 0.05 ng/mL 10 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

2h 10m

sTNF-R ELISA (80kDa) 61-TN8HU-E01 0.16–10 ng/mL 0.10 ng/mL 10 µL

Cell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine

2h 10m

sTNF-R I Monkey ELISA 61-TR1MK-E01 0.09–6 U/mL 0.06 U/mL 10 µL Cell Culture, Serum 2h 10m

sVAP-1 ELISA 61-VAPHU-E0131.3–2,000 pg/mL

19 pg/mL 10 µLAmniotic Fluids, Cell Culture, EDTA Plasma, Heparin Plasma, Serum

3h 10m

87



sVCAM-1 ELISA 61-VCAHU-E01 3.15–100 ng/mL 0.6 ng/mL 100 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

2h 10m

sVE-cadherin ELISA 61-VECHU-E01 0.16–10 ng/mL 0.1 ng/mL 20 µLCell Culture, Heparin Plasma, Serum

3h 10m

sVE-cadherin Monkey ELISA

61-VECMK-E01 0.19–12.5 U/mL 0.10 U/mL 20 µL Cell Culture, Serum 3h 10m

sVEGF-R1/FLT-1 ELISA 61-VGRHU-E01 0.16–10 ng/mL 0.03 ng/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Serum

3h 30m

TGF ß-1 ELISA 20-TGF1HU-E01 19–600 pg/mL 1.9 pg/mL 10 µLCell Culture, Plasma, Serum

6h 45m

TGF ß-2 ELISA 20-TGF2HU-E0131.25–1,000 pg/mL


5h 30m

TGF-ß1 Mouse ELISA 61-TB1MS-E0131.3–2,000 pg/mL


5h 30m

TGF-ß1 Rat ELISA 61-TB1RT-E0131.3–2,000 pg/mL


5h 30m

TGF-ß-2 ELISA 61-TB2HU-E01 31–1,000 pg/mL 6.6 pg/mL 100 µL Cell Culture, Serum 5h 35m

Thiostatin Rat ELISA 41-THIRT-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Serum 2h 10m

Thymulin ELISA 30-9810 0.03–16 ng/mL 0.03 ng/mL 50 µL Serum Overnight

TIE-2 ELISA 61-TIEHU-E01 0.16–10 ng/mL 0.03 ng/mL 10 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

Overnight

TIMP-1 ELISA 61-TIMHU-E01 39–2,500 pg/mL 10 pg/mL 20 µLCell Culture, EDTA Plasma, Heparin Plasma, Serum

3h 10m

TNF-ß ELISA 61-TNBHU-E0115.6–1,000 pg/mL


4h 10m

TNF-α ELISA 45-TNFHU-E05 15–1,500 pg/mL 1.7 pg/mL 100 µLCell Culture, Plasma, Serum

4h

TNF-α ELISA 45-TNFHU-E0115.6–1,500 pg/mL


4h

TNF-α Monkey ELISA 61-TNAMK-E01 28.1–1,800 U/mL 6 U/mL 50 µL Cell Culture, Serum 3h 10m

TNF-α Mouse ELISA 61-TNFMS-E0115.6–1,000 pg/mL


4h 10m

TNF-α Porcine ELISA 45-TNFPO-E01.123.4–1,500 pg/mL


5h

TNF-α Rat ELISA 61-TNART-E0139.1–2,500 pg/mL


Immunology

88




3h


3h

TNF-α-Blocker Monitoring ELISA (Humira®)

30-9657 0.625–20 µg/mL 0.4 µg/L 5 µL Serum 5h 20m

TNF-α-Blocker Monitoring ELISA (Remicade®)

30-9655 0.53–45 µg/mL 0.4 µg/L 5 µL Serum 5h 20m

ToxoPlasma IgG Ab ELISA 27-GD80Cut-off: See Protocol

See Protocol 5 µL Plasma, Serum See Protocol

TRAIL ELISA 61-TRAHU-E0115.6–1,000 pg/mL


3h

VEGF ELISA 45-VEGHU-E01 23–1,500 pg/mL 5 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Serum

4h

VEGF-A ELISA 61-VGAHU-E0115.6–1,000 pg/mL


4h 30m

VEGF-A Mouse ELISA 61-VGAMS-E0162.5–4,000 pg/mL


4h 30m

VEGF-C Rat ELISA 61-VGCRT-E01 47–3,000 pg/mL 27 pg/mL 50 µLCell Culture, Heparin Plasma, Serum

3h 30m

VEGF-R2/KDR ELISA 61-KDRHU-E01 78–5,000 pg/mL 7 pg/mL 50 µLCell Culture, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

3h 30m

VEGF-R3/FLT-4 ELISA 61-FLTHU-E01 0.6–40 ng/mL 0.03 ng/mL 50 µLCell Culture, Citrate Plasma, Serum

3h 30m

Visceral Leishmaniasis ELISA

65-VILHU-E01 See Protocol See Protocol 10 µL Serum 1h 16m

West Nile Virus IgG ELISA 65-WNGHU-E01 See Protocol See Protocol 4 µL Serum 2h 15m

West Nile Virus IgM ELISA 65-WNMHU-E01 * See Protocol See Protocol 4 µL Serum 3h 16m

Zonulin ELISA 30-5600 0–1,000 ng/mL 0.6 ng/mL 250 µL Stool 3h 10m

Zonulin ELISA (Serum) 30-5601 See Protocol 0.6 ng/mL See Protocol Serum 3h 10m

89


Neurobiology

90


Neurobiology brings together many disciplines including biochemistry, molecular

biology, anatomy, physiology and medicine to study the cells of the nervous system

and the organization of these cells into functional circuits that process information and

mediate behavior. ALPCO offers a number of relevant biomarker assays and formats

which can be used for the investigation of neurological function, diseases and disorders

of sensory information processing.

Peripheral Neuropathy

Ganglioside, MAG and SGPG ELISAs can aid in the

differentiation of neurological disorders including

Guillian Barré, Miller Fisher, and chronic inflammatory

demyelinating polyneuropathy.

Gangliosides

Gangliosides are glycosphingolipids containing sialic

acid residues which are particularly abundant in

membrane components of the peripheral nervous

system. Variances in ganglioside structure give rise

to numerous antibody targets where production

of certain anti-ganglioside autoantibodies can be

associated with specific neurological conditions.

Research has shown quantitative detection of these

anti-ganglioside titers may aid in the differential

determination of peripheral neuropathies.1

Neurological disorders can be caused by a variety

of systemic diseases, toxic exposures, medications,

infections, and hereditary disorders.2 Studies have

linked the presence of high anti-ganglioside titers

to several neuropathies including Guillain-Barré

Syndrome (GBS), Miller-Fisher Syndrome (MFS), Acute

Motor Axonal Neuropathy (AMAN), Multifocal Motor

Neuropathy (MMN), and Chronic Ataxic Neuropathy,

Opthalmoplegia, IgM Paraprotein, Agglutinin and

Disialosyl Antibodies (CANOMAD). Additionally,

correlations have been drawn between the severity of

the disorder and the isotype detected. IgG antibodies

Neurobiology

91

have been strongly associated with acute conditions

while IgM antibodies tend to present in chronic diseases.

Many of these neurological disorders exhibit similar

symptoms involving sensory loss and distal pain,

numbness, tingling and weakness, making them difficult

to dissociate. An increasing level of research indicates

immunological tests for ganglioside antibodies plays

an important role in the diagnosis and management of

neuromuscular diseases.3

MAG

The myelin-associated glycoprotein(MAG) is a

transmembrane glycoprotein localized in periaxonal

Schwann cells and ogliodendroglial membranes of myelin

sheaths. IgM antibodies in most neuropathy cases react

to the oligosaccharides (HNK-1 epitope) of glycolipids

and glycoproteins that are concentrated in the peripheral

nerves. This epitope is also shared by a number of

other neuropathy target antigens (i.e. gangliosides,

SGPG). Research has shown detection of anti-MAG IgM

autoantibodies, especially at high titers, is associated with

sensory-motor demyelinating peripheral neuropathies

and IgM gammopathy.4

SGPG

Antibodies recognizing MAG react with a carbohydrate

determinant that is also present on Sulfate-3-glucuronyl

paragloboside (SGPG). While MAG is restricted to

the periaxonal Schwann cell membranes, SGPG has a

wider distribution with presence in both Schwann cell

and neuronal membranes including myelin, axolemma,

and neural endothelial cells. Sensory demyelinating

neuropathies typically exhibit anti-MAG and anti-SGPG

activity but the presence of anti-SGPG antibodies may be

considered more characteristic of a non-demyelinating

motor neuropathy.5

1 Gorenjac, Maksimiljan. Clinical and Diagnostic Role of Ganglioside Antibody Testing. The Journal of The International Federation of Clinical Chemistry and

Laboratory Medicine. 2000; Vol15 No 3. <http://www.ifcc.org/ifccfiles/docs/150309200414.pdf>

2 Azhary, Hend. et al. Peripheral Neuropathy: Differential Diagnosis and Management. American Family Physician. Apr 2010; Vol 81: 887-892.

3 Winner, John. Antibodies and clinical neurological syndromes. The Biomedical Scientist. Jul 2006; 622-624.

4 Czaplinski, A. and Steck, A.J. Antibody testing in peripheral neuropathies. A critical approach. Schweizer Archiv für Neurologie und Psychiatry. Jul 2002; Vol

153 No 7: 301-7.

5 Neuromuscular Clinical Laboratory. Anti-SGPG antibodies. 2012. <http://neuromuscular.wustl.edu/over/labdis.html#sgpg>

Neurobiology

92


5a-Androstane-3a, 17b-diol Glucuronide ELISA

11-ANDHU-E01 0.25–50 ng/mL 0.1 ng/mL 50 µL Serum 45m

5-HIAA ELISA 17-5HIHU-E01 0.5–50 mg/L 0.17 mg/L 50 µL Urine 1h 15m

6-Sulfatoxymelatonin ELISA 01-EK-M6S 0.8–40 ng/mL 0.14 ng/mL 10 µL Urine 4h

α-MSH RIA 13-RB 303 4.7–150 pmol/L 3 pmol/L 100 µLCerebrospinal Fluid, Plasma

Overnight


Anti-GM1 Autoantibodies ELISA

01-EK-GM1-GM ^ Cut-off: See Protocol


Anti-IFN ß Binding Antibodies ELISA

01-EK-IFNB20–500 titer units


Anti-MAG Autoantibodies ELISA

01-EK-MAG ^ 1,000–70,000 titer units


Anti-SGPG Autoantibodies ELISA

01-EK-SGPG ^ Cut-off: See Protocol


Bi-CAT (Epinephrine & Norepinephrine) ELISA

17-BCTHU-E02.1 See Protocol See Protocol 300 µL, 10 µL Plasma, Urine 4h

Anti-Myelin Associated Glycoprotein (MAG) ELISAThe MAG assay utilizes a highly

purified MAG antigen from human

brain and studies have shown this

ELISA to be more sensitive than

western blot.6

6 Kuijf, M.L., et al. Detection of anti-MAG antibodies in polyneuropathy associated with IgM monoclonal gammopathy. Neurology. Sept 2009. Vol 73 No 9: 688-

95.

70605040302010

0

77%

BühlmannELISA

WB-a WB-b

44%54%

Anti-MAG ELISA detected 49 out of 68 total positives for

demyelinating polyneuropathy and IgM gammopathy.

View the anti-MAG assay 01-EK-MAG on page 93

93



Bi-CAT (Epinephrine & Norepinephrine)

Research ELISA17-BCTHU-E02-RES See Protocol See Protocol See Protocol Biological Fluids Overnight

Bi-CAT (Epinephrine & Norepinephrine) RIA

17-BCTHU-R200 See Protocol See Protocol 300 µL, 10 µL Plasma, Urine 4h

Bi-Neph (Metanephrine & Normetanephrine) ELISA

17-BINHU-E02-UFST See Protocol See Protocol 25 µL Urine 1h 45m

Bradykinin ELISA 48-BRAHU-E017.2–5,000 pg/well

7.2 pg/mL 10 µL Plasma, Urine Overnight

cAMP ELISA 55-AMPHU-E020.05–10 pMol/mL

0.1 pMol/mL 50 µL Plasma, Urine 3h 30m

cGMP ELISA 55-GMPHU-E020.05–10 pMol/mL

0.1 pMol/mL 50 µLCell Culture, Plasma, Urine

3h 30m

CGRP ELISA 32-54817.81–1,000 pg/mL

5 pg/mL 100 µL Plasma, Serum Overnight

CGRP Rat ELISA 32-5482 3.91–500 pg/mL 1 pg/mL 100 µLPlasma, Serum, Tissue Culture

Overnight

Chromogranin A ELISA 43-CHRHU-E01.2 31–830 ng/mL 5 ng/mL 50 µL EDTA Plasma 2h 20m

Chromogranin A RIA 13-RB 321 0.156–5 nmol/L 0.04 nmol/L 50 µL Plasma Overnight

CRF ELISA 20-CRFHU-E010.01–1,000 ng/mL


CRF-HS Mouse/Rat ELISA 48-CRFMS-E01 0.078–2.5 ng/mL 0.078 ng/mL 50 µL Plasma, Tissue Culture 8h 30m

Dopamine ELISA 17-DOPHU-E01.1 10–2,000 ng/mL 4.5 ng/mL 300 µL, 10 µL Plasma, Urine 4h

Dopamine Research ELISA 17-DOPHU-E01-RES 0.5–80 ng/mL See Protocol See Protocol Biological Fluids Overnight

Dopamine RIA 17-DOPHU-R100 10–2,000 ng/mL 4.5 ng/mL 300 µL, 10 µL Plasma, Urine 4h

Epinephrine ELISA 17-EPIHU-E01.1 1–200 ng/mL 0.3 ng/mL 300 µL, 10 µL Plasma, Urine 4h

Epinephrine Research ELISA 17-EPIHU-E01-RES 0.5–80 ng/mL See Protocol See Protocol Biological Fluids Overnight

Epinephrine RIA 17-EPIHU-R100 1–200 ng/mL 0.3 ng/mL 300 µL, 10 µL Plasma, Urine 4h

GABA ELISA 17-GABHU-E01 15–1,500 ng/mL 7.5 ng/mL 300 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

Overnight


Antibody ELISA01-EK-GCO




Antibody ELISA (Dual Plate lgG and lgM)

01-EK-GCO-GMCut-off: See Protocol


GanglioCombi® Light Anti-Ganglioside ELISA

01-EK-GCL-GMCut-off: See Protocol


GFAP ELISA 32-5188 0.3–40 ng/mL 0.5 ng/mL 50 µL Cell Culture, Plasma 4h

Neurobiology

94


GanglioCombi® ELISAs The GanglioCombi® ELISA assays

provide a flexible format for IgM

and IgG detection of the most

relevant gangliosides. A proprietary

method for homogenous plate

coating minimizes inter/intra lot

and technician variation, offering a

reliable and cost effective method

for autoimmune ganglioside testing.

Flexible ELISA formats for

differentiation of neurological

disorders

• Mixed IgG/IgM conjugate

for screening

• Individual IgG/IgM conjugates

for differential determination

Simple, reliable, cost effective

method for routine use

• Quantitative

• 30 µL sample size

• Results in 4 hours 30 minutes

Proprietary method for homogenous

plate coating

• Minimal inter/intra lot and

technician variation

• No non-specific binding

Ability to compare results among all

Gangliocombi® assays

• Identical set of calibrator and

controls for all kits

Ganglioside Detection

Conjugate asialo-GM1 GM1 GM2 GD1a GD1b GQ1b

ü ü ü ü ü ü 50:50 Mixed IgG/IgM

ü ü ü ü ü ü Individual IgG and IgM

ü ü ü Individual IgG and IgM

ü Individual IgG and IgM

01-EK-GCO

01-EK-GCO-GM

01-EK-GCL-GM

01-EK-GM1-GM^

95


GHB Enzymatic Assay 01-KK-GHB 5–250 mg/L 1.5 mg/L 8 µL Serum, Urine 8m

Glutamate ELISA 17-GLUHU-E01 0.6–60 µg/mL 0.3 µg/ml 100 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

Overnight

Melatonin Direct Saliva RIA 01-RK-DSM2 0.5–50 pg/mL 0.3 pg/mL 400 µL Saliva Overnight

Melatonin ELISA (Direct Saliva)

01-EK-DSM.1 0.5–25 pg/mL 0.29 pg/mL 200 µL Saliva Overnight

Melatonin RIA 01-RK-MEL2 0.5–50 pg/mL 0.2 pg/mL 1 mL Plasma, Serum Overnight

Metanephrine ELISA (Plasma)

17-METHU-E01-FST 30–3,000 pg/mL 10 pg/mL 250 µL Plasma Overnight

Norepinephrine ELISA 17-NORHU-E01.1 5–1,000 ng/mL 1.5 ng/mL 300 µL, 10 µL Plasma, Urine 4h

Norepinephrine Research ELISA

17-NORHU-E01-RES 0.2–32 ng/mL See Protocol See Protocol Biological Fluids Overnight

Norepinephrine RIA 17-NORHU-R100 5–1,000 ng/mL 1.5 ng/mL 300 µL, 10 µL Plasma, Urine 4h

NPY RIA 13-NPYHU-R100 9.4–300 pmol/L 6 pmol/L 200 µL Plasma, Serum 48h

NSE ELISA 43-NSEHU-E01 5–178 ng/mL 1.2 ng/mL 10 µL Serum 1h 10m

PSA NCAM ELISA 67-ABC0027B 0.25–16 ng/mL 0.25 ng/mL 100 µLCell Culture, Plasma, Serum, Tissue Culture

Overnight

PYY ELISA 48-PYYHU-E01.1 0.082–20 ng/mL 0.082 ng/mL 50 µL Plasma, Serum Overnight

PYY Mouse/Rat ELISA 48-PYYRT-E01.1 0.15–12.5 ng/mL 0.31 ng/mL 25 µLEDTA Plasma, Plasma, Serum

Overnight

S100 ß ELISA 48-CBPHU-E01 0.078–5 ng/mL 0.078 ng/mL 20 µL Plasma 5h 30m

Serotonin ELISA 17-SERHU-E01-FST 15–2,500 ng/mL 5 ng/mL 25 µLCerebrospinal Fluid, Plasma, Platelets, Serum, Urine

1h 15m

Serotonin Research ELISA 17-SERHU-E01-RES 0.015–2.5 ng/mL See Protocol See Protocol Biological Fluids Overnight

Serotonin RIA 17-SERHU-R100 15–2,500 ng/mL 0.3 ng/mL 25 µL, 100 µLCerebrospinal Fluid, Plasma, Platelets, Serum, Urine

2h 15m


Serum Amyloid P Mouse ELISA

41-SAPMS-E01 6.25–200 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 2h 10m

Serum Amyloid P Rat ELISA 41-SAPRT-E01 2.5–160 ng/mL 2.5 ng/mL 5 µL Plasma, Serum 25m

Serum Amyloid ß Auto-Antibody ELISA

20-SABHU-E01 1–120 RTU See Protocol 20 µL Serum 2h 30m

ß Endorphin RIA 13-RB 301 15.6–500 pmol/L 3 pmol/L 3 mL Plasma Overnight

Tri-CAT (Epinephrine, Norepinephrine,

Dopamine) ELISA 17-TCTHU-E03.1 See Protocol See Protocol 300 µL, 10 µL Plasma, Urine 4h

Neurobiology

96



Tri-CAT (Epinephrine, Norepinephrine, Dopamine)

Research ELISA17-TCTHU-E03-RES See Protocol See Protocol See Protocol Biological Fluids Overnight

Tri-CAT (Epinephrine, Norepinephrine,

Dopamine) RIA17-TCTHU-R300 See Protocol See Protocol 300 µL, 10 µL Plasma, Urine 4h

Tryptophan ELISA 17-TRPHU-E01 2.5–250 µg/mL 1.2 µg/mL 20 µL Plasma, Serum, Urine Overnight

Urocortin 2 Mouse ELISA 48-UR2MS-E01 0.81–200 ng/mL 0.82 ng/mL 20 µL Plasma, Serum Overnight

Urocortin 3 Mouse/Rat ELISA

48-UROMS-E01 0.41–100 ng/mL 0.137 ng/mL 25 µL Plasma, Serum Overnight

MelatoninMelatonin is a key modulator of seasonal and circadian

biorhythms. The synthesis and secretion of melatonin is

regulated by a circadian clock in the hypothalamus and is

synchronized by the diurnal light-dark cycle.

Offered exclusively from ALPCO, the Bühlmann portfolio

of melatonin assays utilizes the Kennaway G280 antibody

and provides the most sensitive detection of melatonin

across multiple biological samples.

View melatonin assays 01-RK-DSM2, 01-EK-DSM.1 and 01-RK-MEL2 on page 96

97


Toxicology

98


Toxicological side effects are a common obstacle in drug development pipelines and

often manifest in site-specific damage to vital organs, including the liver, kidney and heart.

Estimates of compound attrition from failure to meet safety endpoints are as high as 45%

in preclinical models and 40% for Phase I clinical trials.1 Now more than ever, labs engaged

in compound, drug and device screening and development are chartered with performing a

comprehensive survey of potential toxicological side effects as part of their routine practice.

ALPCO offers a wide range of preclinical species-specific assays that provide a means for

scientists to achieve their goals in this endeavor.

Hepatotoxicity

DILI can be hepatocellular or choleostatic in nature, but

because viral hepatitis, environmental toxins, or other

diseases present the same pathogenic findings as DILI,

even biopsy is not conclusive.2 Assessment of DILI has

primarily relied on the release of enzymes from necrotic

cells, functional indicators of hepatic activity or increases in

circulating bilirubin.3 Drug-induced liver injury has become

the single most common cause for removal of an approved

drug from market,4 so there continues to be a need for more

specific and sensitive markers of hepatocellular damage.

One such biomarker, α-GST, has been shown to outperform

transaminases as a safety marker in rat models, and may

offer the potential to more accurately assess compound

toxicity by supplementing current screening methods.5

Nephrotoxicity

Until recently, drug-induced acute kidney injury

(DIAKI) has been determined by measurement of

serum creatinine and blood urea nitrogen, both of

which become elevated after damage has occurred.

The advent of novel markers, such as NGAL and π-GST,

are allowing researchers to detect DIAKI earlier and

Toxicology

99

identify the site-specific injury.6,7 The work by the Critical

Path Institute’s Predictive Safety Testing Consortium has

identified and proposed seven new nonclinicalbiomarkers,

KIM-1, Albumin, Total Protein, β2-microglobulin, Cystatin

C, Clusterin and Trefoil Factor-3, for consideration by the

FDA, EMA and PDMA in the drug review process.

Cardiotoxicity

Cardiotoxicity can occur due to exposure to heavy metals,

recreational drugs or pharmaceutical compounds. It

is a common side effect of chemotherapeutic agents,

such as those in the Anthracycline class.9 Even the

newer biologic anticancer therapeutics, such as

Trastuzumab, carry high risks.10 Cardiotoxicity may be

as mild as electrocardiographic changes or arrhythmias,

but can progress to myocardial infraction and even

cardiomyopathy. Biomarkers of cardiomyocyte injury,

like Troponin I, ANP, BNP and CK-MB, may be helpful for

identification of risks associated with these agents.11

1 Suter L, Babiss LE, and Wheeldon EB. Toxicogenomics in predictive toxicology in

drug development. Chem Biology. Feb 2004; Vol 11 No 2: 161-171.

2 U.S. Department of Health and Human Services. Food and Drug Administration.

Center for Drug Evaluation and Research. Guidance for Industry, Drug-

Induced Liver Injury: Premarketing Clinical Evaluation. Washington: Office of

Communications, Division of Drug Information. Jul 2009.


1,25 (OH)2 Vitamin D ELISA 30-2112 5.1–200 pg/mL 4.8 pg/mL 500 µL Plasma, Serum Overnight



α-1-Acid Glycoprotein Chicken ELISA

41-AGPCH-E01 6.25–200 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

α-1-Acid Glycoprotein ELISA

41-AGPHU-E01 5–320 ng/mL 5 ng/mL 5 µL Plasma, Serum 1h 15m

α-1-Acid Glycoprotein Rat ELISA

41-AGPRT-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 20m

α-1-Microglobulin ELISA 30-6710 0.019–1.5 mg/L 0.006 mg/L 10 µL Plasma, Serum, Urine 4h 45m

α-2-Macroglobulin ELISA 30-6610 0.07–5 mg/L 0.05 mg/L 10 µL Plasma, Serum, Stool 2h 15m

α-2-Macroglobulin Rabbit ELISA

41-A2MRB-E01 6.25–400 ng/mL 6.25 ng/mL 5 µL Plasma, Serum 1h 15m

α-2-Macroglobulin Rat ELISA

41-A2MRT-E01 6.25–400 ng/mL 6.24 ng/mL 5 µL Plasma, Serum 1h 20m

α-GST ELISA 69-AGTHU-E01 2.5–80 µg/L 1.9 µg/L 50 µL Plasma, Serum, Urine 2h

Toxicology

100


3 Lee WM and Senior JR. Recognizing drug-induced liver injury: current problems,

possible solutions. Toxicol Pathology. 2005; Vol 33 No 1: 155-164.

4 Temple RJ and Himmel MH. Safety of newly approved drugs: implications for

prescribing. JAMA. May 2002; Vol 287 No 17: 2273–2275.

5 Clarke H, et al. Alpha-glutathione s-transferase (alpha-GST) release, an early

indicator of carbon tetrachloride hepatotoxicity in the rat. Hum Exp Toxicology.

Mar 1997; Vol 16 No 3: 154-157.

6 Mishra J, Ma Q, Prada A, et al. Identification of neutrophil gelatinase-associated

lipocalin as a novel urinary biomarker for ischemic injury. J Am Soc Nephrology.

Oct 2003; Vol 14 No 10: 2534–2543.

7 Koyner JL, et al. Urinary Biomarkers in the Clinical Prognosis and Early

Detection of Acute Kidney Injury. Clin J Am Soc Nephrology. Dec 2010; Vol 5 No

12: 2154-2165.

8 Dieterle F, et al. Renal biomarker qualification submission: a dialog between the

FDA-EMEA and Predictive Safety Testing Consortium. Nat Biotechnology. May

2010; Vol 28 No 5:455-462.

9 Ewer MS and Ewer SM. Cardiotoxicity of anticancer treatments: what the

cardiologist needs to know. Nat Rev Cardiology. Oct 2010; Vol 7 No 10: 564-

575.

10 Cardinale D, et al. Trastuzumab-Induced Cardiotoxicity: Clinical and Prognostic

Implications of Troponin I Evaluation. J Clin Oncology. Sep 2010; Vol 25 No 28:

3910-3916.

11 Gharib MI and Burnett AK. Chemotherapy-induced cardiotoxicity: current

practice and prospects of prophylaxis. Eur J Heart Fail. Jun 2002; Vol 4 No 3:

235-242.


ß-2 Microglobulin ELISA 25-BMGHU-E01 * 0.625–10 µg/mL 0.1 µg/mL 10 µL Serum 1h 20m

ß-2 Microglobulin Rat ELISA 41-MICRT-E01 25–800 ng/mL 25 ng/mL 10 µL Plasma, Serum 1h 30m

AFP ELISA 25-AFPHU-E01 5–300 ng/mL 2 ng/mL 20 µL Serum 1h 20m

Albumin Canine ELISA 41-ALBCA-E01 12.5–400 ng/mL 12.5 ng/mL 5 µL Plasma, Serum 1h 45m

Albumin ELISA 30-6330 12.5–800 µg/L 12.5 µg/L 100 mg, 10 µL Stool, Urine 2h 20m

Albumin Monkey ELISA 41-ALBMK-E01 12.5–400 ng/mL 12.5 ng/mL 100 µL Serum, Tissue Culture 1h 40m

Albumin Mouse ELISA 41-ALBMS-E01 7.8–500 ng/mL 5 ng/mL 2 µL Plasma, Serum, Urine 1h 10m

Albumin Porcine ELISA 41-ALBPO-E01 12.5–400 ng/mL See Protocol 5 µL Plasma, Serum, Urine 1h 40m

Albumin Rat ELISA 41-ALBRT-E01 6.25–400 ng/mL 5 ng/mL 5 µL Plasma, Serum, Urine 1h 10m

Amanitin ELISA 01-EK-AM1 1–100 ng/mL 0.22 ng/mL 40 µL Plasma, Serum, Urine 1h

ANP Rat ELISA 32-510315.6–2,000 pg/mL

15.6 pg/mL 50 µLCitrate Plasma, EDTA Plasma

Overnight

ANP RIA 13-BANI 1 7.8–1,000 pg/mL 3.5 pg/mL 500 µL Plasma Overnight

101



BNP Fragment ELISA (Nt-proBNP 8-29)

04-BI-20852W 5–1,000 fmol/mL 5 fmol/mL 35 µLCell Culture, Plasma, Serum

Overnight

Clusterin Rat ELISA 41-CLURT-E01 12.5–800 ng/mL 12.5 ng/mL 5 µL Plasma, Serum, Urine 1h 30m

Creatine Kinase ELISA 25-CKMHU-E01 7.5–200 ng/mL 7.5 ng/mL 20 µL Serum 1h 20m

Cystatin C ELISA 41-CYCHU-E010.7815–50 ng/mL


Cystatin C Monkey ELISA 41-CYCMK-E01 0.125–4 ng/mL See Protocol 5 µL Plasma, Serum 1h 50m

Cystatin C Mouse ELISA 41-CYCMS-E01 1.25–40 ng/mL 1.25 ng/mL 2 µL Plasma, Serum 1h 40m

Cystatin C PETIA 01-KK-CYC * 0.4–8 mg/L 0.33 mg/L 2 µL Plasma, Serum 10m

Cystatin C PETIA 01-KK-CYC-6 * 0.4–8 mg/L 0.33 mg/L 2 µL Plasma, Serum 10m

Cystatin C Rat ELISA 41-CYCRT-E01 2.63–20 ng/mL 2.63 ng/mL 5 µL Plasma, Serum 1h 45m

Endostatin ELISA 04-BI-20742 0–80 nmol/L 0.2 nmol/L 10 µLCitrate Plasma, EDTA Plasma, Heparin Plasma, Serum

4h 30m

GHB Enzymatic Assay 01-KK-GHB 5–250 mg/L 1.5 mg/L 8 µL Serum, Urine 8m

GST Yb1 Rat ELISA 41-YGTRT-E01 3.12–100 ng/mL 3.12 ng/mL 5 µL Plasma, Serum, Urine 2h 10m













KIM-1 ELISA 41-KIMHU-E01 0.156–10 ng/mL 0.156 ng/mL 100 µL Plasma, Serum, Urine 1h 40m

KIM-1 Mouse ELISA 41-KIMMS-E01 0.078–5 ng/mL 0.078 ng/mL 100 µL Serum, Urine 2h 40m

KIM-1 Rat ELISA 41-KIMRT-E01 0.156–10 ng/mL 0.156 ng/mL 50 µL Serum, Urine 3h 15m

L-FABP ELISA 41-LFAHU-E01 1–32 ng/mL 1 ng/mL 50 µL Plasma, Serum 1h 15m

L-FABP Rat ELISA 41-LFART-E01 12.5–400 ng/mL 12.5 ng/mL 20 µL Plasma, Serum 1h 30m

Microalbumin ELISA 24-MABHU-E01 0.5–100 µg/mL 0.24 µg/mL 10 µL Urine 40m

Toxicology

102



Myoglobin ELISA 25-MYOHU-E01 * 25–1,000 ng/mL 25 ng/mL 20 µL Serum 1h 5m

Myoglobin ELISA 24-MYOHU-E01 0–250 ng/mL 6.25 ng/mL 10 µL Serum, Urine 30m

Myoglobin Mouse ELISA 41-MYOMS-E01 4.69–300 ng/mL 4.69 ng/mL 30 µL Plasma, Serum 1h 40m

Myoglobin Rat ELISA 41-MYORT-E013.125–200 ng/mL


NAG Reagent Set (300) * 73-1290050 0.25–200 U/L 0.25 U/L 10 µL Plasma, Serum, Urine 15m

NAG Reagent Set (5x60) * 73-1085 0.25–200 U/L 0.25 U/L 10 µL Plasma, Serum, Urine 15m

NAG Reagent Set (600) * 73-1290100 0.25–200 U/L 0.25 U/L 10 µL Plasma, Serum, Urine 15m

NAG Spectrophotometric Assay (320)

73-130050 0.16–33 U/L 0.16 U/L 50 µL Plasma, Serum, Urine 30m

NAG Spectrophotometric Assay (640)

73-130100 0.16–33 U/L 0.16 U/L 50 µL Plasma, Serum, Urine 30m


3h 10m


3h 10m


3h 10m


3h 10m


3h 10m


1h

NGAL Rat ELISA 54-046 4–400 pg/mL 0.5 pg/mL 10 µLCell Culture, Plasma, Serum, Tissue Culture, Urine

3h 10m


Osteopontin Mouse ELISA 41-OPNMS-E01 0.03–2 ng/mL 0.015 ng/mL 5 µL Plasma 3h

Pi GST ELISA 69-PGTHU-E01 1.25–40 µg/L 1.3 µg/L 125 µL Urine 2h 15m

Prealbumin ELISA 30-6331 1.6–100 ng/mL 3 ng/mL 100 mg Plasma, Serum, Stool 2h 15m

Prealbumin Mouse ELISA 41-PALMS-E0115.625–500 ng/mL


Prealbumin Rat ELISA 41-PALRT-E0115.625–500 ng/mL


103



proANP ELISA (1-98) 04-BI-20892 0.63–10 nmol/L 0.05 nmol/L 10 µLCell Culture, Plasma, Urine

4h

Procollagen III Peptide RIA 72-OCFK07-PIIIP 0.1–14 U/mL 0.1 U/mL 20 µL Plasma, Serum 5h

RBP4 Mouse ELISA 41-RBPMS-E01 0.625–20 ng/mL See Protocol 5 µL Plasma, Serum 1h 10m

RBP4 Rat ELISA 41-RBPRT-E01 6.25–200 ng/mL See Protocol 5 µL Plasma, Serum, Urine 1h 30m

Type IV Collagen ELISA (Serum)

69-C4SHU-E01 15.6–1,000 µg/L 15.6 µg/L 20 µL Serum 1h

Type IV Collagen ELISA (Urinary)

69-C4UHU-E01 0.8–50 µg/L 0.8 µg/L 50 µL Urine Overnight




41-VDBRT-E013.125–100 ng/mL


Toxicology

104


Immunology

106

Flow Cytometry

106

Products are for Research Use Only unless otherwise noted ‡ Indicates an Analyte Specific Reagent

Flow cytometry is a technique used to evaluate single cell properties, including

relative size, internal complexity and relative fluorescent intensity. Flow cytometry

has applications in a number of fields such as molecular biology, pathology,

immunology and medicine. Within the field of medicine, it is frequently used in

transplantation, hematology and tumor immunology. ALPCO offers a broad range

of unique and affordable flow cytometry solutions that can be used to study any

application of interest.

EuroFlow™ Panels

ALPCO provides validated EuroFlow™ 8-color antibody

panels for immunophenotyping and identification of

hematological malignancies. The single-tube screening

panels and multi-tube classification panels fit into the

EuroFlow™ consortium’s algorithm with entries defined by

the consortium’s standardized laboratory parameters.1

Two groups of markers are combined in each

8-color tube:

1. Backbone markers to identify distinct cell

populations in a sample, and

2. Markers for characterization of specific cell

populations

EuroFlow™ Panels and Infinicyt™ Software

1 Van Dongen JJM et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and

malignant leukocytes. Leukemia. Sep 2012; Vol 26 No 9: 1908–1975.

Flow Cytometry

107107

Single Color Antibodies Catalog # Clone Isotype Excitation Laser

CD1a APC 71-HCD1A-A HI149 IgG1 Red

CD1a PerCP 71-HCD1A-PC HI149 IgG1 Blue

CD2 APC 71-HCD2-A TP1/31 IgG1 Red

CD2 APC-C750 70-2AC750 LT2 IgG2b Red

CD2 CF-Blue 71-HCD2-CB TP1/31 IgG1 Violet

CD2 PerCP 71-HCD2-PC TP1/31 IgG1 Blue

CD3 APC 71-HCD3-A 33-2A3 IgG2a Red

CD3 APC 70-3AP6 33-2A3 IgG2a Red

CD3 APC-C750 70-3AC750 UCHT-1 IgG1 Red

CD3 CF-Blue 71-HCD3-CB UCHT1 IgG1 Violet

CD3 DY-634 71-HCD3-DY 33-2A3 IgG2a Red

CD3 FITC 70-3F8 Cris-7 IgG2a Blue

CD3 OC-515 71-HCD3-OC 33-2A3 IgG2a Violet

CD3 OC-515 71-HCD3-OC.2 UCHT1 IgG1 Violet

CD3 PE 70-3PE5 33-2A3 IgG2a Blue

CD3 PerCP 71-HCD3-PC 33-2A3 IgG2a Blue

CD4 APC 71-HCD4-A HP2/6 IgG2a Red

CD4 FITC 70-4F5 Edu-2 IgG2a Blue

In multi-tube panels, the backbone markers are optimally

placed at the same fluorochrome position in every tube

in order to provide identical multidimensional localization

of the target cell population(s). The characterization

markers are positioned according to the utility of the

combined markers.

Infinicyt™

Infinicyt™ is a flow cytometry analysis software program

that provides an innovative approach for data integration

and multidimensional analysis of flow cytometry data. Its

state-of-the-art features make analysis and interpretation

of results easier, faster and more accurate. It is

recommended by the EuroFlow™ Consortium.

Immunology

108

Flow Cytometry

108



CD4 OC-515 71-HCD4-OC HP2/6 IgG2a Violet

CD4 Pacific Blue 70-4PBZ ‡ H2P/6 IgG2a Violet

CD4 PE 71-HCD4-PE HP2/6 IgG2a Blue

CD4 PerCP 71-HCD4-PC HP2/6 IgG2a Blue

CD4 PerCP-Cy5.5 70-4C5 ‡ SK3 IgG1 Blue

CD5 APC 71-HCD5-A 33-1C5 IgG2a Red

CD5 CF-Blue 71-HCD5-CB 33-1C5 IgG2a Violet

CD5 OC-515 71-HCD5-OC 33-1C5 IgG2a Violet

CD5 PerCP 71-HCD5-PC 33-1C5 IgG2a Blue

CD5 PerCP-Cy5.5 70-5C ‡ UCHT2 IgG1 Blue

CD6 APC 71-HCD6-A MAE-1C10 IgG1 Red

CD6 Biotin 71-HCD6-B MAE-1C10 IgG1 n/a

CD6 CF-Blue 71-HCD6-CB MAE-1C10 IgG1 Violet

CD6 FITC 71-HCD6-F MAE-1C10 IgG1 Blue

CD6 PE 71-HCD6-PE MAE-1C10 IgG1 Blue

CD7 APC 70-7AP4 ‡ CD7-6B7 IgG2a Red

CD7 APC 71-HCD7-A HIT7 IgG1 Red

CD7 Biotin 71-HCD7-B HIT7 IgG1 n/a

CD7 CF-Blue 71-HCD7-CB HIT7 IgG1 Violet

CD7 FITC 71-HCD7-F HIT7 IgG1 Blue

CD8 APC 71-HCD8-A 143-44 IgG1 Red

35-DSMHU-E01 3 - 300 U/mL 1.0 U/mL Serum

CD8 APC 71-HCD8-A.2 MEM-31 IgG2a Red

CD8 Biotin 71-HCD8-B 143-44 IgG1 n/a

CD8 CF-Blue 71-HCD8-CB 143-44 IgG1 Violet

CD8 DY-634 71-HCD8-DY 143-44 IgG1 Red

CD8 FITC 70-8F8 ‡ UCHT-4 IgG2a Blue

CD8 OC-515 71-HCD8-OC 143-44 IgG1 Violet

109109



CD8 PerCP 71-HCD8-PC 143-44 IgG1 Blue

CD9 APC 71-HCD9-A VJ1/20 IgG2a Red

CD9 Biotin 71-HCD9-B VJ1/20 IgG2a n/a

CD9 CF-Blue 71-HCD9-CB VJ1/20 IgG2a Violet

CD9 OC-515 71-HCD9-OC VJ1/20 IgG2a Violet

CD9 PerCP 71-HCD9-PC VJ1/20 IgG2a Blue

CD10 APC 71-HCD10-A HI10a IgG1 Red

CD10 APC-C750 70-10AC750 ‡ HI10a IgG1 Red

CD10 CF-Blue 71-HCD10-CB HI10a IgG1 Violet

CD10 FITC 71-HCD10-F HI10a IgG1 Blue

CD10 PerCP 71-HCD10-PC HI10a IgG1 Blue

CD11a APC 71-HCD11A-A TP1/40 IgG1 Red

CD11a CF-Blue 71-HCD11A-CB TP1/40 IgG1 Violet

CD11a OC-515 71-HCD11A-OC TP1/40 IgG1 Violet

CD11b APC 71-HCD11B-A DCIS1/18 IgG2a Red

CD11b CF-Blue 71-HCD11B-CB DCIS1/18 IgG2a Violet

CD11b FITC 71-HCD11B-F DCIS1/18 IgG2a Blue

CD11b OC-515 71-HCD11B-OC DCIS1/18 IgG2a Violet

CD11b PerCP 71-HCD11B-PC DCIS1/18 IgG2a Blue

CD11c PerCP 71-HCD11C-PC BU-15 IgG1 Blue

CD14 APC 70-14AP3 ‡ 47-3D6 IgG1 Red

CD14 Biotin 71-HCD14-B 47-3D6 IgG1 n/a

CD14 CF-Blue 71-HCD14-CB 47-3D6 IgG1 Violet

CD14 OC-515 71-HCD14-OC 47-3D6 IgG1 Violet

CD14 PerCP 71-HCD14-PC 47-3D6 IgG1 Blue

CD15 CF-Blue 71-HCD15-CB MCS-1 IgG1 Violet

CD15 PerCP 71-HCD15-PC MCS-1 IgG1 Blue

CD16 APC 71-HCD16-A 3G8 IgG1 Red

Immunology

110

Flow Cytometry

110



CD16 Biotin 71-HCD16-B 3G8 IgG1 n/a

CD16 CF-Blue 71-HCD16-CB 3G8 IgG1 Violet

CD16 FITC 71-HCD16-F 3G8 IgG1 Blue

CD16 FITC 71-HCD16-F.2 GRM1 IgG1 Blue

CD16 PE 71-HCD16-PE 3G8 IgG1 Blue

CD16 PerCP 71-HCD16-PC 3G8 IgG1 Blue

CD18 APC 71-HCD18-A GRF1 IgG1 Red

CD18 Biotin 71-HCD18-B GRF1 IgG1 n/a

CD18 CF-Blue 71-HCD18-CB GRF1 IgG1 Violet

CD18 FITC 71-HCD18-F GRF1 IgG1 Blue

CD18 OC-515 71-HCD18-OC GRF1 IgG1 Violet

CD19 APC 71-HCD19-A A3-B1 IgG2a Red

CD19 CF-Blue 71-HCD19-CB A3-B1 IgG2a Violet

CD19 FITC 70-19F4 HD37 IgG1 Blue

CD19 PECy5 70-19C2 J4.119 IgG1 Blue

CD19 PECy7 70-19C5 ‡ J4.119 IgG1 Blue

CD19 PerCP 71-HCD19-PC A3-B1 IgG2a Blue

CD19 PerCP-Cy5.5 70-19C7 HIB19 IgG1 Blue

CD20 APC 71-HCD20-A LT20 IgG1 Red

CD20 Biotin 71-HCD20-B LT20 IgG1 n/a

CD20 FITC 70-20F3 2H7 IgG2b Blue

CD20 Pacific Blue 70-20PBZ ‡ 2H7 IgG2b Violet

CD20 PerCP 71-HCD20-PC LT20 IgG1 Blue

CD21 APC-C750 70-21AC750 ‡ B-ly4 IgG1 Red

CD21 CF-Blue 71-HCD21-CB HI21a IgG2a Violet

CD21 FITC 71-HCD21-F HI21a IgG2a Blue

CD22 APC 71-HCD22-A HIB22 IgG1 Red

CD22 APC-C750 70-22AC750 ‡ MEM-01 IgG1 Red

111111



CD22 CF-Blue 71-HCD22-CB HIB22 IgG1 Violet

CD22 FITC 71-HCD22-F HIB22 IgG1 Blue

CD22 PE 71-HCD22-PE HIB22 IgG1 Blue

CD23 FITC 71-HCD23-F B-G6 IgG1 Blue

CD24 APC 71-HCD24-A SN3 IgG1 Red

CD25 APC 71-HCD25-A TP1/6 IgG2b Red

CD25 CF-Blue 71-HCD25-CB TP1/6 IgG2b Violet

CD25 PerCP 71-HCD25-PC TP1/6 IgG2b Blue

CD26 APC 71-HCD26-A TP1/19 IgG2b Red

CD26 Biotin 71-HCD26-B TP1/19 IgG2b n/a

CD26 CF-Blue 71-HCD26-CB TP1/19 IgG2b Violet

CD26 PerCP 71-HCD26-PC TP1/19 IgG2b Blue

CD28 FITC 71-HCD28-F KOLT-2 IgG1 Blue

CD29 APC 71-HCD29-A VJ1/14 IgG2b Red

CD29 Biotin 71-HCD29-B VJ1/14 IgG2b n/a

CD29 CF-Blue 71-HCD29-CB VJ1/14 IgG2b Violet

CD29 OC-515 71-HCD29-OC VJ1/14 IgG2b Violet

CD29 PerCP 71-HCD29-PC VJ1/14 IgG2b Blue

CD31 Biotin 71-HCD31-B TP1/15 IgG2a n/a

CD31 CF-Blue 71-HCD31-CB TP1/15 IgG2a Violet

CD31 OC-515 71-HCD31-OC TP1/15 IgG2a Violet

CD33 APC 71-HCD33-A HIM3-4 IgG1 Red

CD33 Biotin 71-HCD33-B HIM3-4 IgG1 n/a

CD33 CF-Blue 71-HCD33-CB HIM3-4 IgG1 Violet

CD36 FITC 71-HCD36-F TR9 IgG1 Blue

CD36 PE 71-HCD36-PE TR9 IgG1 Blue

CD38 APC-C750 70-38AC750 ‡ LD38 IgG1 Red

CD38 CF-Blue 71-HCD38-CB GR7A4 IgG1 Violet

Immunology

112

Flow Cytometry

112



CD38 DY-634 71-HCD38-DY GR7A4 IgG1 Red

CD38 FITC 70-38F LD38 IgG1 Blue

CD38 OC-515 71-HCD38-OC GR7A4 IgG1 Violet


CD40 Biotin 71-HCD40-B HI40a IgG1 n/a



CD41 APC 71-HCD41-A HIP8 IgG1 Red

CD41 Biotin 71-HCD41-B HIP8 IgG1 n/a

CD41 CF-Blue 71-HCD41-CB HIP8 IgG1 Violet

CD41 FITC 71-HCD41-F HIP8 IgG1 Blue

CD41 PerCP 71-HCD41-PC HIP8 IgG1 Blue

CD42a APC 71-HCD42A-A GR-P IgG2a Red

CD42a Biotin 71-HCD42A-B GR-P IgG2a n/a

CD42a CF-Blue 71-HCD42A-CB GR-P IgG2a Violet

CD42a FITC 71-HCD42A-F GR-P IgG2a Blue

CD42a OC-515 71-HCD42A-OC GR-P IgG2a Violet

CD42a PE 71-HCD42A-PE GR-P IgG2a Blue

CD42a PerCP 71-HCD42A-PC GR-P IgG2a Blue

CD42a PURE 71-HCD42A-PU GR-P IgG2a n/a

CD42b APC 71-HCD42B-A HIP1 IgG1 Red

CD42b Biotin 71-HCD42B-B HIP1 IgG1 n/a

CD42b CF-Blue 71-HCD42B-CB HIP1 IgG1 Violet

CD42b FITC 71-HCD42B-F HIP1 IgG1 Blue

CD42b PerCP 71-HCD42B-PC HIP1 IgG1 Blue

CD43 Biotin 71-H43-B TP1/36 IgG1 n/a

CD43 CF-Blue 71-H43-CB TP1/36 IgG1 Violet

CD43 OC-515 71-HCD43-OC TP1/36 IgG1 Violet

113113



CD43 PerCP 71-HCD43-PC TP1/36 IgG1 Blue




CD45 APC-C750 70-45AC750 ‡ GA90 IgG2a Red

CD45 CF-Blue 71-HCD45-CB D3/9 IgG1 Violet

CD45 CF-Blue 71-HCD45-CB.2 HI30 IgG1 Violet

CD45 DY-634 71-HCD45-DY D3/9 IgG1 Red

CD45 OC-515 71-HCD45-OC D3/9 IgG1 Violet

CD45 OC-515 70-45OC ‡ GA90 IgG2a Violet

CD45 PECy5 71-HCD45-PEC5 IMMU19.2 IgG1 Blue

CD45 PECy5 70-45C3 MMU19.2 IgG1 Blue

CD45 PerCP 71-HCD45-PC D3/9 IgG1 Blue

CD45 PerCP 71-HCD45-PC.2 HI30 IgG1 Blue

CD45RA APC 71-HCD45RA-A HI100 IgG1 Red

CD45RA CF-Blue 71-HCD45RA-CB GRT22 IgG1 Violet

CD45RA CF-Blue 71-HCD45RA-CB.2 HI100 IgG1 Violet

CD45RA PerCP 71-HCD45RA-PC HI100 IgG1 Blue

CD45RB APC 71-HCD45RB-A MC5/2 IgG1 Red

CD45RB CF-Blue 71-HCD45RB-CB MC5/2 IgG1 Violet

CD45RB FITC 71-HCD45RB-F MC5/2 IgG1 Blue

CD45RC APC 71-HCD45RC-A RP1/12 IgG1 Red

CD45RC FITC 71-HCD45RC-F RP1/12 IgG1 Blue

CD45RC PURE 71-HCD45RC-PU RP1/12 IgG1 n/a

CD49b APC 71-HCD49B-A TEA1/41 IgG1 Red

CD49b CF-Blue 71-HCD49B-CB TEA1/41 IgG1 Violet

CD49b FITC 71-HCD49B-F TEA1/41 IgG1 Blue

CD49b PE 71-HCD49B-PE TEA1/41 IgG1 Blue

Immunology

114

Flow Cytometry

114



CD49c APC 71-HCD49C-A VJ1/6 IgG1 Red

CD49c CF-Blue 71-HCD49C-CB VJ1/6 IgG1 Violet

CD49c FITC 71-HCD49C-F VJ1/6 IgG1 Blue

CD49c PE 71-HCD49C-PE VJ1/6 IgG1 Blue

CD49d APC 71-HCD49D-A ALC1/1 IgG1 Red

CD49d Biotin 71-HCD49D-B ALC1/1 IgG1 n/a

CD49d CF-Blue 71-HCD49D-CB ALC1/1 IgG1 Violet

CD49d FITC 71-HCD49D-F ALC1/1 IgG1 Blue

CD49d PerCP 71-HCD49D-PC ALC1/1 IgG1 Blue

CD52 APC 71-HCD52-A HI186 IgG2b Red

CD52 Biotin 71-HCD52-B HI186 IgG2b n/a

CD52 CF-Blue 71-HCD52-CB HI186 IgG2b Violet

CD52 FITC 71-HCD52-F HI186 IgG2b Blue

CD52 PE 71-HCD52-PE HI186 IgG2b Blue

CD52 PerCP 71-HCD52-PC HI186 IgG2b Blue

CD53 APC 71-HCD53-A HI29 IgG1 Red

CD53 Biotin 71-HCD53-B HI29 IgG1 n/a

CD53 CF-Blue 71-HCD53-CB HI29 IgG1 Violet

CD53 FITC 71-HCD53-F HI29 IgG1 Blue

CD53 PE 71-HCD53-PE HI29 IgG1 Blue

CD53 PerCP 71-HCD53-PC HI29 IgG1 Blue

CD56 APC 71-HCD56-A B-A19 IgG1 Red

CD56 APC-C750 70-56AC750 ‡ C5.9 IgG2b Red

CD56 Biotin 71-HCD56-B B-A19 IgG1 n/a

CD56 PE 70-56PE ‡ C5.9 IgG2b Blue

CD56 PURE 71-HCD56-PU B-A19 IgG1 n/a

CD57 FITC 71-HCD57-F HI57a IgM Blue

CD57 PURE 71-HCD57-PU HI57a IgM n/a

115115





CD58 FITC 71-HCD58-F HI58a IgG1 Blue

CD58 PE 71-HCD58-PE HI58a IgG1 Blue

CD59 APC 71-HCD59-A VJ1/12,2 IgG2a Red

CD59 Biotin 71-HCD59-B VJ1/12,2 IgG2a n/a

CD59 CF-Blue 71-HCD59-CB VJ1/12,2 IgG2a Violet

CD59 FITC 71-HCD59-F VJ1/12,2 IgG2a Blue

CD59 OC-515 71-HCD59-OC VJ1/12,2 IgG2a Violet

CD59 PE 71-HCD59-PE VJ1/12,2 IgG2a Blue

CD59 PerCP 71-HCD59-PC VJ1/12,2 IgG2a Blue

CD61 PerCP 71-HCD61-PC C17 IgG1 Blue

CD62E APC 71-HCD62E-A TEA2/1 IgG2b Red

CD62E FITC 71-HCD62E-F TEA2/1 IgG2b Blue

CD62L APC 71-HCD62L-A HI62L IgG2a Red

CD62L CF-Blue 71-HCD62L-CB HI62L IgG2a Violet

CD62L PerCP 71-HCD62L-PC HI62L IgG2a Blue

CD62P APC 71-HCD62P-A P.seK02.22 IgG2b Red

CD62P CF-Blue 71-HCD62P-CB P.seK02.22 IgG2b Violet

CD62P PerCP 71-HCD62P-PC P.seK02.22 IgG2b Blue

CD63 APC 71-HCD63-A TEA3/18 IgG1 Red

CD63 Biotin 71-HCD63-B TEA3/18 IgG1 n/a

CD63 CF-Blue 71-HCD63-CB TEA3/18 IgG1 Violet

CD63 FITC 71-HCD63-F TEA3/18 IgG1 Blue

CD63 OC-515 71-HCD63-OC TEA3/18 IgG1 Violet

CD63 PE 71-HCD63-PE TEA3/18 IgG1 Blue

CD63 PerCP 71-HCD63-PC TEA3/18 IgG1 Blue

CD64 PE 71-HCD64-PE 10.1 IgG1 Blue

CD69 APC 71-HCD69-A FN50 IgG1 Red

Immunology

116

Flow Cytometry

116



CD71 CF-Blue 71-HCD71-CB FG2/12 IgG1 Violet

CD71 FITC 71-HCD71-F FG2/12 IgG1 Blue

CD71 PerCP 71-HCD71-PC FG2/12 IgG1 Blue

CD81 APC-C750 70-81AC750 ‡ M38 IgG1 Red

CD86 FITC 71-HCD86-F BU63 IgG1 Blue

CD98 APC 71-HCD98-A FG1/8 IgG1 Red

CD98 Biotin 71-HCD98-B FG1/8 IgG1 n/a

CD98 CF-Blue 71-HCD98-CB FG1/8 IgG1 Violet

CD98 PE 71-HCD98-PE FG1/8 IgG1 Blue

CD98 PerCP 71-HCD98-PC FG1/8 IgG1 Blue

CD99 APC 71-HCD99-A HI156 IgG2a Red

CD99 Biotin 71-HCD99-B HI156 IgG2a n/a

CD99 CF-Blue 71-HCD99-CB HI156 IgG2a Violet

CD99 FITC 71-HCD99-F HI156 IgG2a Blue

CD99 PerCP 71-HCD99-PC HI156 IgG2a Blue

CD105 APC 71-H105A-PC 2H6F11 IgG1 Red

CD105 CF-Blue 71-HCD105-CB 2H6F11 IgG1 Violet

CD105 OC-515 71-HCD105-OC 2H6F11 IgG1 Violet

CD105 PerCP 71-HCD105-PC 2H6F11 IgG1 Blue

CD117 APC 71-HCD117-A 104D2 IgG1 Red

CD138 APC-C750 70-138AC750 B-A38 IgG1 Red

CD138 FITC 70-138F4 B-A39 IgG2 Blue

CD138 PE 70-138PE4 B-A38 IgG1 Blue

CD147 APC 71-HCD147-A VJ1/9 IgG1 Red

CD147 CF-Blue 71-HCD147-CB VJ1/9 IgG1 Violet

CD147 FITC 71-HCD147-F VJ1/9 IgG1 Blue

CD147 PE 71-HCD147-PE VJ1/9 IgG1 Blue

CD147 PerCP 71-HCD147-PC VJ1/9 IgG1 Blue

CD235a APC 71-HC235A-A HI264 IgG2a Red

117117



CD235a CF-Blue 71-HC235A-CB HI264 IgG2a Violet

CD235a FITC 71-HC235A-F HI264 IgG2a Blue

CD235a PE 71-HC235A-PE HI264 IgG2a Blue

Anti Caspase-3 active PURE 71-HCASP3-PU IgG n/a

Anti-cKit PURE 71-HCKIT-PU IgG n/a

Anti-GST PURE 71-HGST-PU IM14/21 IgG n/a

Anti-IL8 PURE 71-HIL8-PU IL8-1505 IgG2a n/a

Anti-LMO1 PURE 71-HLMO1-PU LMO1-1201 IgG2a n/a

CPA APC 71-HCPA-A CA2 IgG Red

CycCD3 Pacific Blue 70-3PBZ ‡ UCHT-1 Violet

CyCD79a PE 70-79APE ‡ HM57 IgG1 Blue

CyMPO FITC 70-MPOF3 ‡ 2C7 IgG1 Blue

cyTCRβ APC 70-bF1AP 8A3 IgG1 Red

HLA-DP APC 71-HHLADP-A HI43 IgG1 Red

HLA-DP CF-Blue 71-HHLADP-CB HI43 IgG1 Violet

HLA-DP FITC 71-HHLADP-F HI43 IgG1 Blue

HLA-DP PE 71-HHLADP-PE HI43 IgG1 Blue

HLA-DP PURE 71-HHLADP-PU HI43 IgG1 n/a

HLA-DQ APC 71-HHLADQ-A HI118 IgG1 Red

HLA-DQ CF-Blue 71-HHLADQ-CB HI118 IgG1 Violet

HLA-DQ FITC 71-HHLADQ-F HI118 IgG1 Blue

HLA-DR APC 71-HHLADR-A GRB-1 IgG2a Red

HLA-DR Biotin 71-HHLADR-B GRB-1 IgG2a n/a

HLA-DR CF-Blue 71-HHLADR-CB GRB-1 IgG2a Violet

HLA-DR FITC 71-HHLADR-F GRB-1 IgG2a Blue

HLA-DR OC-515 71-HHLADR-OC GRB-1 IgG2a Violet

HLA-DR PE 71-HHLADR-PE GRB-1 IgG2a Blue

HLA-DR PerCP 71-HHLADR-PC GRB-1 IgG2a Blue

Immunology

118

Flow Cytometry

118



IREM-2 FITC 71-HIREM2-F UP-H2 IgG2a Blue

IREM-2 PerCP 71-HIREM2-PC UP-H2 IgG2a Blue

Kappa APC 70-KappaP Polyclonal Red

Kappa FITC 70-KAPPF2 Polyclonal Blue

Kappa PE 70-KAPPPE ‡ Polyclonal Blue

Lambda APC-C750 70-LAC750 ‡ Polyclonal Red

Lambda FITC 70-LAMBF ‡ Polyclonal Blue

Lambda APC 70-LAMBAP Polyclonal Red

Lambda APC 71-HLAMBD-A HIgλ IgG6 Red

Lambda-PE 70-LAMBPE Polyclonal Blue

Lambda CF-Blue 71-HLAMBDA-CB Higλ IgG7 Violet

Multicolor Combinations Catalog # Clone Isotype Excitation Laser

CD3 FITC/CD4 PE 70-3F8-4PE9 Cris-7, Edu-2 IgG2a, IgG2a Blue

CD3 FITC/CD8 PE 70-3F8-8PE4 Cris-7, 17D8 IgG2a, IgG1 Blue

CD3 FITC/CD16 PE 70-3F8-16PE7 Cris-7, 3G8 IgG2a , IgG1 Blue

CD3 FITC/CD19 PE 70-3F8-19PE5 Cris-7, HD37 IgG2a, IgG1 Blue

CD3 FITC/CD56 PE 70-3F8-56PE Cris-7, C5.9 IgG2a, IgG2b Blue

CD3 FITC/HLADR PE 70-3F8-DRPE Cris-7, 423L IgG2a, IgG2a Blue

CD4 FITC/CD8 PE 70-4F6-8PE4 Edu-2, 17D8 IgG2a, IgG1 Blue

CD4 FITC/CD45 PE 70-4F6-45PE4 Edu-2, D3/9 IgG2a, IgG1 Blue

CD5 FITC/CD19 PE 70-5F4-19PE5 MCD5, HD37 IgG2b, IgG1 Blue

CD8 FITC/CD16 PE 70-8F8-16PE7 UCH-T4, 3G8 IgG2a, IgG1 Blue

CD8 FITC/CD56 PE 70-8F8-56PE UCH-T4, C5.9 IgG2a, IgG2b Blue

CD45 FITC/CD14PE 70-45F5-14PE3 EO1, 47-3D6 gG2a, IgG1 Blue

HLA B27 FITC/HLA B7 PE 70-COMB1012 HLA ABC m3, BB7.1 IgG2a, IgG1 Blue

119119



IgG1 FITC/IgG1 PE 70-IC5F5PE MCG1, MCG1 Blue

IgG1 FITC/IgG2a PE 70-IC5F6PE MCG1, MCG2a Blue

IgG1 FITC/IgG2b PE 70-IC5F7PE MCG1, MCG2b Blue

IgG2a FITC/IgG1 PE 70-IC6F5PE MCG2a, MCG1 Blue

IgG2a FITC/IgG2a PE 70-IC6F6PE MCG2a, MCG2a Blue

IgG2a FITC/IgG2b PE 70-IC6F7PE MCG2a, MCG2b Blue

IgG2b FITC/IgG1 PE 70-IC7F5PE MCG2b, MCG1 Blue

IgG2b FITC/IgG2a PE 70-IC7F6PE MCG2b, MCG2a Blue

IgG2b FITC/IgG2b PE 70-IC7F7PE MCG2b, MCG2b Blue

Kappa FITC/CD19 PE 70-KF2-19PE5 Blue

Kappa FITC/LAMBDA PE 70-KF2-LPE Blue

Lambda FITC/CD19 PE 70-LF-19PE5 Blue

LAMBDA FITC/Kappa PE 70-LF-KPE Blue

CD3 FITC/CD4 PE/CD8 PECy5 70-3F8-4PE9-8C2 Cris-7, Edu-2, B9.11 IgG2a, IgG2a, IgG1 Blue

CD3 FITC/CD4 PE/CD19 PECy5 70-3F8-4PE9-19C2 Cris-7, Edu-2, J4.119 IgG2a, IgG2a, IgG1 Blue

CD3 FITC/CD4 PE/CD45 PECy5 70-3F8-4PE9-45C3 Cris-7, Edu-2, IMMU19.2 IgG2a, IgG2a, IgG1 Blue

CD3 FITC/CD8 PE/CD19 PECy5 70-3F8-8PE4-19C2 Cris-7, 17D8, J4.119 IgG2a, IgG1, IgG1 Blue

CD3 FITC/CD8 PE/CD45 PECy5 70-3F8-8PE4-45C3 Cris-7, 17D8, IMMU19.2 IgG2a, IgG1, IgG1 Blue

CD3 FITC/CD16 PE/CD19 PECy5 70-3F8-16PE7-19C2 Cris-7, 3G8, J4.119 IgG2a, IgG1, IgG1 Blue

CD3 FITC/CD16+56 PE/CD19 PECy570-3F8-16PE7+56PE-19C2

Cris-7, 3G8+C5.9, J4.119 IgG2a, IgG1+IgG2b, IgG1 Blue

CD3 FITC/CD16+56 PE/CD45 PECy570-3F8-16PE7+56PE-45C3

Cris-7, 3G8+C5.9, IMMU19.2 IgG2a, IgG1+IgG2b, IgG1 Blue

CD3 FITC/CD19 PE/CD45 PECy5 70-3F8-19PE5-45C3 Cris-7, HD37, IMMU19.2 IgG2a, IgG1, IgG1 Blue

CD3 FITC/CD56 PE/CD19 PECy5 70-3F8-56PE-19C2 Cris-7, C5.9, J4.119 IgG2a, IgG2b, IgG1 Blue

CD4 FITC/CD8 PE/CD3 PECy5 70-4F6-8PE4-3C4 Edu-2, 17D8, UCHT1 IgG2a, IgG1, IgG1 Blue

CD4 FITC/CD8 PE/CD45 PECy5 70-4F6-8PE4-45C3 Edu-2, 17D8, IMMU19.2 IgG2a, IgG1, IgG1 Blue

CD8 FITC/CD4 PE/CD3 PECy5 70-8F8-4PE9-3C4 UCH-T4, Edu-2, UCHT1 IgG2a, IgG2a, IgG1 Blue

CD8 FITC/CD4 PE/CD19 PECy5 70-8F8-4PE9-19C2 UCH-T4, Edu-2, J4.119 IgG2a, IgG2a, IgG1 Blue

Immunology

120

Flow Cytometry

120



CD8 FITC/CD4 PE/CD45 PECy5 70-8F8-4PE9-45C3 UCH-T4, Edu-2, IMMU19.2 IgG2a, IgG2a, IgG1 Blue

CD10 FITC/CD5 PE/CD19 PECy5 70-10F1-5PE4-19C2 SN5c, MCD5, J4.119 IgG1, IgG2b, IgG1 Blue

CD16 FITC/CD19 PE/CD3 PECy5 70-16F6-19PE5-3C4 3G8, HD37, UCHT1 IgG1, IgG1, IgG1 Blue

CD56 FITC/CD19 PE/CD3 PECy5 70-56F-19PE5-3C4 C5.9, HD37, UCHT1 IgG2b, IgG1, IgG1 Blue

FMC7 FITC/CD23 PE/CD19PECy5 70-FMC7F-23PE3-19C2 FMC7, B-G6, J4.119 IgM, IgG1, IgG1 Blue

IgG1 FITC/IgG1 PE/IgG1 PECy5 70-IC5F5PE5C MCG1, MCG1, ZX3 Blue

IgG1 FITC/IgG2a PE/IgG1 PECy5 70-IC5F6PE5C MCG1, MCG2a, ZX3 Blue

IgG1 FITC/IgG2b PE/IgG1 PECy5 70-IC5F7PE5C MCG1, MCG2b, ZX3 Blue

IgG2a FITC/IgG1 PE/IgG1 PECy5 70-IC6F5PE5C MCG2a, MCG1, ZX3 Blue

IgG2a FITC/IgG2a PE/IgG1 PECy5 70-IC6F6PE5C MCG2a, MCG2a, ZX3 Blue

IgG2a FITC/IgG2b PE/IgG1 PECy5 70-IC6F7PE5C MCG2a, MCG2b, ZX3 Blue

IgG2b FITC/IgG1 PE/IgG1 PECy5 70-IC7F5PE5C MCG2b, MCG1, ZX3 Blue

IgG2b FITC/IgG2a PE/IgG1 PECy5 70-IC7F6PE5C MCG2b, MCG2a, ZX3 Blue

IgG2b FITC/IgG2b PE/IgG1 PECy5 70-IC7F7PE5C MCG2b, MCG2b, ZX3 Blue

Kappa FITC/Lambda PE/CD19 PECy5 70-KF2-LPE-19C2 Polyclonal, Polyclonal, J4.119 Polyclonal, Polyclonal, IgG1 Blue

Kappa FITC/Lambda PE/CD19 PECy5 70-KF2-LPE-19C2-50 Polyclonal, Polyclonal, J4.119 Polyclonal, Polyclonal, IgG1 Blue

Lambda FITC/Kappa PE/CD19 PECy5 70-LF-KPE-19C2 Polyclonal, Polyclonal, J4.119 Polyclonal, Polyclonal, IgG1 Blue

CD45 FITC/CD8 PE/CD4 PECy5/

CD3 APC 70-45F5-8PE4-4C3-3AP6 EO1, 17D8, 13B8.2, GA60 IgG2a, IgG1, IgG1, IgG1 Blue, Red

121121


Product Catalog # Description

Studies of Major Lymphocyte Subpopulations (Two Color)

70-SPLC-25CD8 + SmIgλ-FITC CD56 + SmIgK-PE

Studies of Major Lymphocyte Subpopulations (Three Color)

70-SPLC4-25CD8 + SmIgλ-FITC CD56 + SmIgK-PE CD4-PerCP-Cy5.5

Product Catalog #

Basic Module Infinicyt™ 70-INFINICYT-P

Advanced Module Infinicyt™ 70-INFINICYT-ADV1

EuroFlow™ Validated Panels Infinicyt™ Data Analysis Software

Product Catalog # Description

Acute Leukemia Orientation Tube (ALOT-7) 70-ALOT77 conjugated antibodies designed for initial assessment of the nature of immature populations of hematopoietic cells in acute leukemia samples in order to allow appropriate orientation towards the complementary B, T lineage, and AML/MDS antibody panels.

Small Sample Screening Tube (SST) 70-SST10

11 conjugated antibodies designed for the detection of normal and aberrant lymphocyte populations of B, T and NK lineage by flow cytometry. This 8-color panel can be used for evaluation of “small” samples and samples with (very) low cell counts, such as fine needle aspirates (FNA), cerebrospinal fluid (CSF), vitreous humor, etc. with a suspicion of primary lymphoma.

Lymphoid Screening Tube (LST-11) 70-LST11

11 conjugated antibodies designed for the detection of aberrant mature lymphocyte populations of B, T and NK lineage by flow cytometry. This 8-color panel can be used for evaluation of several suspected conditions, such as lymphocytosis, lymph node enlargement, splenomegaly, monoclonal serum components, unexplained cytopenias, etc.

EuroFlow™ Validated Screening Tubes

Immunology

122

Flow Cytometry

122


123123

Gastroenterology

124


Type Description Catalog #

Equipment Quantum Blue® Lateral Flow Reader 02-BI-POCTR-ABS ^

Columns 11,12-DHET Immunoaffinity Columns 60-DH32

Columns Angiotensin II/Vasopressin Extraction Columns 02-A22/B-AEC

Columns Chromobond Columns 30-C-SE2112

Columns GLP-1 Sample Extraction Columns 43-GLPHU-SE50

Columns Melatonin Extraction Columns 02-MEL/B-MEC

Saliva Collection Salivettes (Sterile Saliva Collection Devices) 02-MEL/B-SVC

Saliva Collection SleepCheck Saliva Melatonin Collection Kit 01-B-SLEEPCHECK16

Stool Collection EasySampler® Stool Collection Kit 58-EZSAMPLER

Stool Collection Histamine ELISA Stool Collection Device 17-BA-D-1241

Stool Collection & Extraction ScheBo® Quick-Prep Fecal Sample Extraction Kit 02-B-CAL-SO50

Stool Collection & Extraction Smart-Prep Fecal Sample Extraction Kit 02-B-CAL-RD

Other Basiskit for LIA 14-HD-371.0

Other Care™ Fecal Occult Blood Rapid Testing Rack 43-FOBHU-RACK

Other Microplate Rotator (4 plate) 06-S2020-P4

Other Microplate Seal Film 06-SEAL-FILM

Other Non-Sterile Dispenser Syringe Tips 06-ST-3


125


5a-Androstane-3a, 17b-diol Glucuronide 48, 93

5-HIAA 93

6-Sulfatoxymelatonin 93

8-isoPGF2a 29

20-HETE 28

α-1-Acid Glycoprotein (AGP) 66, 100

α-1-Antichymotrypsin 48, 58

α-1-Microglobulin 100

α-2-Macroglobulin 100

α-Fodrin 58, 66

α-MSH 48, 93

ß-2 Microglobulin 101

ß-4 Thymosin 87

ß-Catenin 66, 76

ß hCG 48

A

A Proliferation Inducing Ligand (APRIL) 71

Acid Labile Subunit 48

AcSDKP 29

Adenosine 29

Adenovirus 6

Adipocyte Fatty Acid Binding Protein 38

ADH 35, 54

ADH/AVP 35, 54

Adiponectin 38, 42

ADMA 29, 30

Adrenocorticotropic Hormone (ACTH) 29, 48, 93

AKT1/2 66

Albumin 39, 100, 101

Aldosterone 28, 34, 48

Alpha-1 Thymosin 59

Alpha-Fetoprotein (AFP) 48, 101

Alpha-Lactalbumin 66

Amanitin 101

ANA 66, 75

Androstenedione 48

Angiotensin 28, 30, 34, 48, 125

Angiotensin Converting Enzyme (ACE) 28, 29, 48, 66

Annexin V 66

ANP 29, 30, 100, 101

Anti-ANCA 66

Anti-BPI 66

Anti-Cag-A 6

Anti-Candida 6

Anti-Cardiolipin 67

Anti-Casein 6, 59, 67

Anti-CaSR 67

Anti-CCP (CCPlus Immunoscan®) 72, 73

Anti-CENP-B 67

Anti-Cytomegalovirus 6, 7, 67

Anti-ds-DNA 67

Anti-Elastase 67

Anti-Gliadin 7, 59, 67

Anti-GM1 Autoantibodies 93

Anti-Helicobacter Pylori 7, 8, 59, 60, 68

Anti-Histone 68

Anti-IFN ß 68, 93

Anti-Inositol-GM 68

Anti-Insulin Autoantibodies (AIA) 39, 40, 66, 68, 81

Anti-Intrinsic Factor 68

Anti-Jo-1 69

Anti-LC1 69

Anti-LKM-1 69

Anti-M2 69

Anti-MAG 69, 93

Anti-Ovalbumin 8, 60, 69

Anti-Ovarian Antibody 69

Antioxidative Capacity 30

Anti-Phosphatidyl-Serine 69

Anti-Phospholipid-8 69

Anti-Prothrombin 69

Anti-Rheumatoid Factor (RF) 69

Anti-Ps-Aeruginosa 8

Anti-Ribosomal P 69

Anti-Rubella 8, 69

Anti-Saccharomyces Cerevisiae (Crohns Check) 61, 75

Anti-Scl 70 70

Anti-SGPG 70, 92, 93

Anti-SLA 70

Anti-Sm 70

Anti-Soya 8, 60, 70

Anti-Sperm Antibody 48, 70

Anti-SS 70

Anti-ß 2 Glycoprotein 70

Anti-ss-DNA ELISA 70

Anti-ß Lactoglobulin 70

126

IndexIndex

Antithrombin 30, 70

Anti-Thyroid Peroxidase Antibody (TPO) 49, 54

Anti-U1-70 71

AOPP 30

Apolipoprotein 30

ASCA 66, 71

Aspergillus 8, 17, 71

AVP 35, 54

B

BAFF 71

Basophil Activation (FlowCAST®) 10, 61

Bcl-2 71

Biotin 60, 109, 110, 111, 112, 113, 115, 116, 117, 118

Blastocystis Antigen 71

BNP 29, 30, 100, 102

Bone Sialoprotein 22

Bordetella Pertussis 8, 71

Bradykinin 30, 94

Brucella 8

C

C4a 71

C5a 71

Calcitonin 22, 49

Calcitonin Gene Related Peptide (CGRP) 94

Calprotectin 57, 58, 60, 71, 72

cAMP 94

Cancer Antigen 72, 73

Carbonylated Protein 31

Cardiolipin 67, 73

Cartilage Oligomeric Matrix Proetin (Hcomp) 23

Casomorphin 8, 60

Caspase 73, 118

Cathepsin 22, 67, 73

CD153 73

CEA 73

Cell Adhesion Molecule (L1-CAM) 82

Cell Proliferation & Toxicity 74

Cellular Adhesion Molecule (sICAM-2) 87

Cellular Adhesion Molecule (sICAM-3) 87

Cellular Adhesion Molecule (sVCAM-1) 88

Ceruloplasmin 31

CETP 31

cGMP 94

Chemerin 74

Chlamydia 6, 9, 74

Cholecystokinin (CCK) 39, 49

Chromogranin A 49, 94

Chrorionic Gonadotropin (hCG) 48, 50

Chymotrypsin 61, 74

Ciculating Immune Complex (C1q) 67, 74

CINC-1 74

Citrullinated Protein Antibodies (CPA) 75, 118

Clusterin 100, 102

c-Met 49

Complement Factor 3 74

Complement System 74, 75

Connective Tissue Factor (CTGF) 23, 75

Corticosterone 49

Corticotropin Releasing Factor (CRF) 31, 94

Cortisol 49

C-Peptide 39

C-Reactive Protein (CRP) 74, 75

Creatine Kinase ELISA 31, 102

CREB ELISA 75

Cripto-1 75

CRP 31, 74, 75

C. Trachomatis 8, 71

Cu/ZnSOD 75

Cystatin C 100, 102

Cytochrome C 75

Cytokeratin Fragment (CYFRA) 75

D

DAO 9, 31, 61

Dengue 9, 75

Deoxypyridinoline (DPD) 23

DHEA 49

DHET 28, 125

DHT 49

DKK-1 23

Dopamine 94, 96, 97

127

E

Echinococcus Granulosus 9

Endostatin 31, 102

Endothelin 31

Eosinophil Derived Neurotoxin (EDN) 61

Eotaxin 76

Epidermal Growth Factor (EGF) 76

Epinephrine 93, 94, 96, 97

Epstein Barr Virus 9

ERK1/2 76

Erythropoietin (EPO) 31, 49

Estradiol 48, 49

Estriol 49

Estrone 49

F

Fasciola Hepatica 9

Ferritin 31, 49

Fetuin-A 23, 31

FGF 39, 50, 76

FGF-21 39, 76

Fibrinogen 31, 32

Fibronectin 32

Folicle Stimulating Hormone (FSH) 50

G

GABA 94

GAD 39, 76

Galectin-3 32, 76

GanglioCombi® Anti-Ganglioside 76, 94

Gastrin RIA 39, 61

G-CSF 76

GFAP 94

GHB 96, 102

Ghrelin 38, 39

Gliadorphin 61

Glicentin 39

Glomerular Basement Membrance (GBM) 30, 67

GLP-1 39, 41, 125

GLP-2 40

Glucagon 40, 41

Glutamate 16, 96

Glutathione 32, 40, 50

GM-CSF 76

GPx Activity 32

Granzyme 76

Growth Hormone 50, 51

GSK3α/ß 66, 76

GST 32, 99, 100, 101, 102, 103, 118

H

Haptoglobin 32, 61, 76, 77, 102

HAV 7, 67, 68

HBc 68

HBs 68

HBsAg 77

HCV 7, 68

Hemoglobin 32, 61

Hemopexin 33

Hepcidin 33, 50, 51

HER-2 77

Herpes 8, 10, 68, 77

Histamine 10, 77, 125

Homocysteine 33, 51

Human Anti-Human Antibody (HAHA) 76

Human Placental Lactogen (HPL) 51

Hydroxy Pyridinium Crosslinks (PYD/DPD) 23

I

IFN-gamma 77

IgA 6, 7, 8, 9, 10, 11, 12, 58, 59, 60, 61, 62, 66, 67, 68, 69,

70, 71, 73, 74, 75, 77, 78, 86

IgD 78

IgE 5, 6, 7, 78

IGF-1 50, 51

IGF-2 52

IGFBP-1 52

IGFBP-2 51, 52

IGFBP-3 51, 52

IgG 6, 7, 8, 9, 10, 11, 12, 39, 58, 59, 60, 61, 66, 67, 68,

69, 70, 71, 72, 73, 74, 75, 78, 81, 82, 83, 84, 86, 89, 91,

95, 118

IgG1 78, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117,

118, 119, 120, 121

IgG2A 78

IgG2B 78

IgG3 78

128

Index

IgM 6, 7, 8, 9, 10, 11, 12, 60, 67, 68, 69, 70, 73, 74, 75, 77,

78, 82, 83, 89, 91, 92, 93, 95, 115, 121

IgY 78

IL-1RA 80

IL-1ß 40, 80

IL-1α 80

IL-2 80

IL-3 80

IL-4 81

IL-5 81

IL-6 23, 24, 40, 81

IL-8 40, 81

IL-9 81

IL-10 78, 79

IL-12 79

IL-13 ELISA 79

IL-15 79

IL-17A 79

IL-17AF 79

IL-17F 79

IL-18 79

IL-21 80

IL-22 80

IL-23 79, 80

IL-27 80

IL-29 80

IL-31 80

IL-33 81

Influenza 10

Insulin 39, 40, 41, 42, 44, 50, 51, 68, 81

IP-10 81

Islet Cell Antibody 42, 81

J

Japanese Encephalitis 10, 81, 82

K

Kappa 82, 119, 120, 121

KIM-1 100, 102

Krytopyrrole 33

L

Lactoferrin 61, 82

Lambda 82, 119, 120, 121

L-Citrulline 82

Lecithin-Cholesterol Acyltransferase (LCAT) 33

Leptin 42

Leukemia Inhbitory Factor 82

Leukotriene Function (CAST®) 8, 9, 60, 61

L-FABP 102

Lipoprotein 33

LPL 33

Luteinizing Hormone 52

Lysozyme 61, 62

M

Malondialdehyde 33

Matrix Metalloproteinase (MMP) 82

MCP-1 42, 82

Measles 10, 82

Melatonin 96, 97, 125

Metanephrine 94, 96

Microalbumin 42, 102

MPO ELISA 33, 69, 82

MRP 8/14 ELISA 33, 62, 82

Mumps 11

Mycobacterium Tuberculosis 11, 82

Mycoplasma Hominis 11

Myoglobin 33, 103

Myostatin 24, 43, 52

N

NAG 103

Neopterin 82, 83

Neuron Specific Enolase (NSE) 96

Neuropeptide Y (NPY) 43, 96

NGAL 43, 82, 83, 99, 103

Nitric Oxide Synthase 1 (NOS1) 33

Nitrotyrosine 33

Norepinephrine 93, 94, 96, 97

Normetanephrine 94

Nt-proCNP 24, 33

Nucleo-h 83

O

Obestatin 43

Orientia Tsutsugamushi 11, 83

129

Osteocalcin 24

Osteopontin 24, 84, 103

Osteoprotegerin 24, 33

Ovotransferrin 84

Oxidized LDL 34

OxyStat 34

P

p38 α 84

p53 84

PAI-1 43, 84

Pancreatic Amylase 43

Pancreatic Polypeptide 43

PAP Complex 84

PAPP-A 52, 84

Parvovirus B 19 11

Pepsinogen 43, 62

Pertussis 8, 71, 84

Phospholipase 2 34

Plasminogen 34

Platelet Derived Growth Factor 84

PMN Elastase 84

Porphyrins 84

PR3 ANCA 69

Prealbumin 52, 103

Pregnant Mare Serum Gonadotropin (PMSG) 52

Pregnenolone 52

Procollagen III Peptide 24, 104

Progesterone 48, 52, 53

Progranulin 84

Proinsulin ELISA 43

Prolactin 53

PSA 53, 84, 96

PYY 43, 96

R

RANTES/CCL5 84

RBP 43

RC4d 84, 85

Relaxin 53

Renin 28, 34, 35, 48, 53

Resistin 44

Respiratory Syncytial Virus 11

S

s90K/Mac-2BP 84

S100A12 62

S100 ß 96

sAPO-1/Fas 84

sCD23 85

sCD26 85

sCD28 85

sCD30 85

sCD40 85

sCD40L 85

sCD44std 86

sCD44var 86

sCD134 84

sCD137 84

sCD152/CTLA-4 85

Sclero-Profile 86

Sclerostin 22, 23, 24, 25, 44

Secretory IgA 62, 86

SeroMP 86

Serotonin 96

Serum Amyloid 35, 44, 86, 96

sE-selectin 86

sFas Ligand 86

SHBG 53

sICAM-1 86, 87

sL-selectin 87

Somatostatin 44, 53

sPECAM-1 87

sP-selectin 87

sPSGL-1 87

sRANKL 25

STELLUX™ 38, 41, 44

sTNF-R 87

sVE-cadherin 88

Syphilis 11

T

T4 17, 53, 119, 120, 121

TBG 54

Testosterone 54

Tetanus Toxin 11

Thyroglobulin (TG) 48, 54

130

Index

TGF ß-1 88

TGF ß-2 88

Thiostatin 88

Thymulin 88

TIE-2 88

TIMP-1 88

Tissue Transglutaminase 60, 61, 67, 71, 74

TNF Related Apoptosis Inducing Ligand (TRAIL) 89

TNF-ß 88

TNF-α 44, 45, 88, 89

Toxoplasma 11

Transferrin 35

Triiodothyronine (T3) 17, 53

Troponin I 35, 100, 101

Tryptophan 97

TSH 54

Type IV Collagen 104

U

Ubiquinone Coenzyme Q10 35

Ureaplasma Urealyticum 12

Urocortin 97

Urotensin II 35

V

Varicella Zoster 12

Vascular Adhesion Protein (sVAP-1) 87

Vascular Endothelial Growth Factor (VEGF) 89

Vasoactive Intestinal Peptide 54, 63

Vasopressin (ADH/AVP) 35, 54, 125

Vaspin 45

Visceral Leishmaniasis 12, 89

Vitamin A 35, 63

Vitamin B1 62, 63

Vitamin B2 62

Vitamin B3 62

Vitamin B5 63

Vitamin B6 35, 63

Vitamin B9 61

Vitamin B12 63

Vitamin C 63

Vitamin D 22, 25, 48, 58, 63, 100, 104

Vitamin E 35, 63

Vitamin K1 25, 63

W

West Nile Virus 9, 12, 89

Z

Zonulin 63, 89

131

Ordering Online

Please visit our website at www.alpco.com to set up

an account and place an online order. ALPCO accepts

MasterCard, Visa and American Express. If you choose

to set up an online account and want Terms, you will

be contacted by a Customer Service Associate upon

placing your first order to complete a credit application.

Establishing a new customer account

To establish a new customer account with Terms,

contact Customer Service to obtain a credit application.

Completed applications can be emailed to cs@alpco.

com or faxed to (603)898-6854. Upon acceptance of

a completed credit application, you will be provided

with an 8 character Customer I.D. number, (i.e.

ALPCO=ALPCO001) for ordering purposes.

Orders by Fax

Faxed orders can be sent to (603)898-6854. Be

sure to include your Customer I.D. number, special

instructions and price quotation when applicable to

ensure proper pricing.

Orders by Phone

To order by phone, simply supply our Customer

Service staff with your ALPCO customer ID number,

a valid purchase order number or valid credit card, the

appropriate catalog numbers and the required quantities.

Iodination Dates

ALPCO has developed a schedule of iodination dates for

all radio-labeled assay kits to ensure that customers can

receive the freshest kits possible. This allows ALPCO

customers to effectively plan their usage. Contact ALPCO

Customer Service at [email protected] or call (800)592-

5726 to obtain iodination schedule information.

Call today for more information or to place an order by phone. You can also submit

online orders by visiting www.alpco.com.

132

IndexOrdering Information

Licensing and Warranty

In compliance with US Federal regulations, all

customers ordering radioactive products must submit a

copy of the institution’s most current and valid radiation

license to ALPCO. This documentation will remain on

file for future ordering.

All products manufactured and/or distributed by

ALPCO should be used in accordance with the

labeled intended use. All products that are labeled

“For Research Use Only. Not for Use in Diagnostic

Procedures.” are supplied for research applications and

not for use in diagnostic or clinical use. Please contact

ALPCO for current product status.

ALPCO, it’s manufacturers and/or distributors make no

warranties either expressed or implied concerning any

matter whatsoever, including without limitations, the

condition of the product, its merchantability, or its fitness

for any particular use. Any modification of a test as well

as exchange or mixture of any components from different

lots may influence results, and cannot be supported.

Terms of Sale

Terms of Sale are net 30 days from date of invoice. Report

all incidents of shortage, breakage or any product damage

within 5 days of receipt of goods to ALPCO at www.alpco.

com, through our Resources/Contact Us feature or calling

(800) 592-5726. Should damage occur during shipping,

please report damages to both the carrier and ALPCO.

ALPCO assumes no liability for loss or delay caused by

conditions beyond ALPCO’s control. Report all shipping

errors to ALPCO immediately. Due to the nature of the

products listed herein, all sales are considered final. No

consideration will be given to returns or replacements

unless prior authorization and shipping instructions have

been obtained from ALPCO Customer Service.

Shipping

Shipping and handling charges are (FOB/EXW: Salem,

NH 03079)and prepaid and added to your invoice

total. ALPCO uses standard overnight service on most

domestic shipments.

Information on hot deal discounts, new product promotions, and special offers can

be found on the Promotions section of the ALPCO website. Order soon because

supplies are limited!

133

ALPCO

26-G Keewaydin Drive

Salem, NH 03079

Phone: 800-592-5726

Fax: 603-898-6854

[email protected]

www.alpco.com

2013–2014

catalogo alpco

Documents

new products

research use

featured products

obesity research

research sections

product line

technical tips

scientific solutions