cation-exchange anion-exchange chemical name structural … · a : ch3oh / 10 mmol/l k2hpo4 (ph...

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper

Acetic Acid 2 ＊

N -Acetyl-2,3-didehydro-2-deoxyneuraminic Acid

39 *

N -Acetylneuraminic Acid 1, 39 * * *

N -Acetylneuraminic AcidMethyl Ester

39 *

N -Acetylserotonin 48 *

Adenosine Diphosphate(ADP)

4, 5, 49 ＊＊＊

Adenosine Monophosphate(AMP)

4, 5, 6,49

＊＊＊

Adenosine Triphosphate(ATP)

4, 5, 49 ＊＊＊

Adrenaline 8 ＊＊＊

Alanine 9 ＊

Allantoin 10 ＊＊

Alprenolol 11,12,13 ＊＊＊

-Aminobutyric Acid(GABA)

14 ＊＊＊

4-(Aminomethyl)benzoic Acid 42 *

Arginine 9 ＊

Chemical Name Structural Formula Page.

Cation-Exchange

A

1 / 8

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper


Cation-Exchange

L-Ascorbic Acid(Vitamin C)

2, 3, 41,51

＊＊＊

Aspartic Acid 9 ＊

Benzene 15, 16, 17 ＊＊＊

Benzenesulfonic Acid 15, 16, 17 ＊＊＊

Benzoic Acid 40, 51 *

Benzonitrile 18 ＊＊＊

Bis[(trifluoromethyl)sulfonyl]amine Anion

(CF3SO2)2N- 37 *

Buformin 38 *

1-Butyl-3-methylimidazoliumChloride

34 ＊

Caffeine 19 ＊＊＊

Chlorpromazine 13 ＊

Citric Acid 2 ＊

Clomipramine 13 ＊

Creatine 43 *

Creatinine 8 ＊＊＊

A

B

C

2 / 8

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper


Cation-Exchange

4-Cyanobenzoic Acid 42 *

Cyclic AdenosineMonophosphate (cAMP)

4, 49 ＊＊＊

Cytidine Monophosphate（CMP）

6, 7 ＊＊＊

Dehydroacetic Acid 51 *

Dichloroacetic Acid 20 ＊

Dicyanamide Anion (NC)2N- 36 *

3,5-Dihydroxybenzoic Acid 18 ＊＊＊

3,5-Diiodo-L-thyronine 50 *

N ,N -Dimethylsulfamide 52 *

DOPA 8 ＊＊＊

Dopamine 8,18 ＊＊＊

Erythorbic Acid 41 * * *

Ethylbenzene 15, 16, 17 ＊＊＊

4-Ethylbenzene sulfonic Acid 15, 16, 17 ＊＊＊

2-Ethyl-4-methylimidazole 21 ＊

D

E

C

SO

N NH2O

3 / 8

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper


Cation-Exchange

E 1-Ethyl-3-methylimidazolium 36, 37 *

F Fumaric Acid 45 *

GABASee Page.1

"-Aminobutyric Acid" 14

Galacturonic Acid 2,3 ＊＊＊

2-O-α-D-Glucopyranosyl-L-ascorbic Acid

41 * * *

Glucose 1-Phosphate 47 *

D-Glucose 6-Phosphate 47 *

L-Glutamic Acid 14 ＊＊＊

Glutathione oxidized form(GSSG)

44 *

Glutathione reduced form(GSH)

44 *

N -Glycolylneuramic Acid 1, 39 ＊＊＊

Glyphosate 22 ＊＊＊

Guanosine Monophosphate(GMP)

6 ＊＊

H Hexafluorophosphate PF6- 37 *

G

4 / 8

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper


Cation-Exchange

Histamine 23, 24 ＊＊

Homovanillic Acid(HVA)

8 ＊＊＊

3-Hydrazinobenzoic Acid 40 *

4-Hydrazinobenzoic Acid 40 *

5-Hydroxyindole-3-aceticAcid

48 *

5-Hydroxy-L-tryptophan 25, 48 ＊＊＊

Inosine Monophosphate(IMP)

6 ＊＊

Isobutyl 4-Hydroxybenzoate 27 ＊

Isoproterenol 8 ＊＊＊

Lactic Acid 2 ＊

Levulinic Acid 28 ＊

Lithium Chloride LiCl 33 ＊

Maleic Acid 45 *

Malic Acid 2, 45 ＊

Melamine 29 ＊＊＊

Melatonin 48 *

L

M

H

I

5 / 8

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper


Cation-Exchange

Metformin 26, 27, 38 ＊＊＊

Methionine 9 ＊＊

Methyl Levulinate 28 ＊

4-Methyl-1H -benzotriazole 46 *

5-Methyl-1H -benzotriazole 46 *

2-Methylimidazole 21 ＊

Monochloroacetic Acid 20 ＊

NAD+(Nicotinamide AdenineDinucleotide)

30 ＊＊

Nicardipine 11, 12, 13 ＊＊＊

Nicotinic Acid 30 ＊＊

Noradrenaline 8 ＊＊＊

D-Panthenol 10 ＊＊

Phenformin 38 *

2-Phenoxyethanol 51 *

P

N

M

6 / 8

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper


Cation-Exchange

Phenylalanine 9 ＊

L-Phenylephrine 19 ＊＊＊

Phosphocreatine 43 *

Potassium Bromide KBr 32, 35 ＊

Potassium Chloride KCl 32, 33 ＊＊

Potassium Iodide Kl 32, 35 ＊

Serotonin (5-HT) 8, 48 ＊＊

Sodium Chloride NaCl 33 ＊

Sorbic Acid 51 *

Succinic Acid 45 *

Tartaric Acid 45 *

Tetrafluoroborate Anion BF4- 36 *

DL-Thyronine 50 *

L-Thyroxine (T4) 50 *

Toluene 15, 16, 17 ＊＊

p -Toluenesulfonic Acid 15, 16, 17 ＊＊＊

P

T

S

7 / 8

Anion-Exchange

ODS-CX10

ODS-CX15

ODS-CX20

ODS-AX20

ODS-AX10

ODS-SAXSuper


Cation-Exchange

Toluidine (o ,m ,p ) 31 ＊＊＊

Tranexamic Acid 42 *

Trichloroacetic Acid 20 ＊

Trifluoromethanesulfonate CF3SO3- 36 *

(Trifluoromethyl)trifluoroborateAnion CF3BF3

- 36, 37 *

3,3',5-Triiodo-L-thyronine (T3) 50 *

L-Tryptophan 48 *

Tyramine 8 ＊＊＊

UUridine Monophosphate(UMP)

6,7 ＊＊＊

Vanillylmandelic Acid(VMA)

8 ＊＊＊

Verapamil 11, 12, 13 ＊＊＊

Vitamin CSee Page.1

"L-Ascorbic Acid"2, 3, 41,

51

T

V

8 / 8

-1-

Sialic Acids

Column : Kaseisorb LC ODS-SAX Super Column : TCI Dual ODS-AX10 Column : TCI Dual ODS-AX20 Column : Kaseisorb LC ODS 2000

Column Size 4.6 mmI.D. X 150 mm

Mobile Phase CH3CN / 10 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 5 / 95

Detection UV 210 nm

Flow Rate 1.0 mL/min

Temperature 30 °C

Sample

1. N-Glycolylneuramic acid

2. N-Acetylneuramic acid

O

OH

COOH

HO

HN

HO OH

HO

HOH2C

O

O

OH

COOH

HO

HN

HO OH

HO

O

0.0 2.5 5.0 7.5 10.0 12.5 min

1

1

1

1

2

2

2

2

-2-

Organic Acids

Sample : Galacturonic Acid

Sample : Ascorbic Acid

Sample : Lactic Acid

Sample : Acetic Acid

Sample : Malic Acid

Sample : Citric Acid

Column TCI Dual ODS-AX20


Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH7.0, H3PO4) = 20 / 80

Detection UV 210 nm


Temperature 40 °C

Sample

Galacturonic Acid

-Ascorbic Acid (Vitamin C)

Lactic Acid

Acetic Acid

Malic Acid

Citric Acid

OH

OH

O

OH

OHO

OH

OHO

O

O

OH OH

O

OH

O

OH

OH OH

O OHO

OH

O

OH

HO

COOH

OH

HO

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 min

-3-

Organic Acids

Column : Kaseisorb LC ODS-SAX Super Column : TCI Dual ODS-AX10 Column : TCI Dual ODS-AX20 Column : Kaseisorb LC ODS 2000


Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH7.0, H3PO4) = 10 / 90

Detection UV 210 nm


Temperature 40 °C

Sample

1. Galacturonic Acid

2. -Ascorbic Acid (Vitamin C)

0.0 2.5 5.0 7.5 min

1

1

1

1,2

2

2

2

OH OH

O OHO

OHO

OH

HO

COOH

OH

HO

-4-

Adenosine Phosphates

Column: Kaseisorb LC ODS-SAX Super

Column: TCI Dual ODS-AX10


Column: Kaseisorb LC ODS 2000


Mobile Phase

A : CH3OH / 10 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80

B : CH3OH / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80

0-100 % B (10-30 min)

Detection UV 254 nm


Temperature 40 °C

Sample

1. AMP 3. ADP

2. cAMP 4. ATP

N

NN

N

NH2

OH OH

OOP

O

HO

OH

N

NN

N

NH2

O OH

OO

PO

OH

N

NN

N

NH2

OH OH

OOP

O

O

OH

PHO

O

OH

N

NN

N

NH2

OH OH

OOPO

O

OH

PO

O

OH

PHO

O

OH

1

0 10 20 30 40 min

1

1

1

2

2

2

23

3

3

4,3

4

4

4

-5-

Nucleotides (Adenosine Phosphates)

Column: Kaseisorb LC ODS-SAX Super





Mobile Phase CH3CN / 20 mmol/L NH4HCO3 (pH 7.8) = 20 / 80

Detection UV 254 nm


Temperature 40 °C

Sample

1. AMP 3. ATP

2. ADP

N

NN

N

NH2

OH OH

OOP

O

HO

OH

N

NN

N

NH2

OH OH

OOP

O

O

OH

PHO

O

OH

N

NN

N

NH2

OH OH

OOPO

O

OH

PO

O

OH

PHO

O

OH

ADP and ATP is not elution.

0.0 5.0 10.0 15.0 20.0 25.0 min

1

1

1

1,2,3

2

2

3

3

-6-

Nucleotides Column : TCI Dual ODS-AX10 Column : TCI Dual ODS-AX20


Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH 7.0,H3PO4) = 20 / 80

Detection UV 254 nm


Temperature 40 °C

Sample

1. CMP

2. UMP

3. IMP

4. GMP 5. AMP

0 5 10 15 (min)

1

2

3,4

5

1 2

3

4

5

0 5 10 15 (min)

N

NH2

ON

O

OHOH

OPHO

OH

O

NH

O

ON

O

OHOH

OPHO

OH

O

N

NHN

N

O

OH OH

OOP

O

HO

OH

N

NHN

N

O

OH OH

OOP

O

HO

OH

NH2 N

NN

N

NH2

OH OH

OOP

O

HO

OH

-7-

Nucleotides

Column : Kaseisorb LC ODS-SAX Super

Column : TCI Dual ODS-AX10


Column : Kaseisorb LC ODS 2000


Mobile Phase CH3OH / 10 mmol/L K2HPO4 (pH 7.0, H3PO4) = 10 / 90

Detection UV 254 nm


Temperature 40 °C

Sample

1. CMP (Cytidine Monophosphate）

2. UMP (Uridine Monophosphate)

N

NH2

ON

O

OHOH

OPHO

OH

O

NH

O

ON

O

OHOH

OPHO

OH

O

0.0 2.5 5.0 7.5 10.0 12.5 min

1

1,2

1

1,2

2

2

-8-

Catecholamines

Column: TCI Dual ODS-CX20

Column: TCI Dual ODS-CX15 Column: TCI Dual ODS-CX10

Column Size 4.6 mmI.D. × 150 mm

Mobile Phase CH3CN / 50 mmoL/L HCOONH4 (pH 3.5, HCOOH) = 70 / 30

Detection UV 254 nm


Temperature 25 ℃

Sample

1. VMA

2. DOPA

3. HVA

4. Noradrenaline

5. Adrenaline

6. Creatinine

7. Dopamine

8. Serotonin

9. Tyramine

10. Isoproterenol

OH

O

HOOH

O

NH2

OH

OHO

OHO

OH

OH

O

OH

OH

NH2

HO

OH

OH

HONH

NHN

O

NH

OH

OH

NH2

NH

HO

NH2

OH

NH2

OH

HN

HO

OH

2

2

2,4,5,78,9,10

3

3

3 4

4

5

5

1

1

1 6

6

7

7

8

8 9

9 10

10

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 min

-9-

Amino Acids

Sample : Aspartic Acid

Sample : Alanine

Sample : Methionine

Sample : Phenylalanine

Sample : Arginine

Column TCI Dual ODS-CX15


Mobile Phase

A: CH3CN / 5 mmol/L KH2PO4 (pH 3.0, H3PO4) = 5 / 95

B: CH3CN / 50 mmol/L KH2PO4 (pH 3.0, H3PO4) = 40 / 60

0-100 % B (5-20 min)

Detection UV 210 nm


Temperature 30 °C

Sample

Aspartic Acid

Alanine

Methionine

Phenylalanine

Arginine

HOOH

NH2

O

O

OH

O

NH2

SOH

O

NH2

OH

NH2

O

H2N NHOH

NH O

NH2

0.0 5.0 10.0 15.0 20.0 25.0 min

-10-

Allantoin, D-Panthenol

Column : TCI Dual ODS-CX10 Column : TCI Dual ODS-CX20



Detection UV 210 nm


Temperature 30 °C

Sample

1. Allantoin

2. D-Panthenol

0 4 8 12 (min)

0 4 8 12 (min)

NH

NH

NH

O

H2N O

O

HO NH

O

OH

1

1

2

2

-11-

Basic Drugs







Detection UV 254 nm


Temperature 40 °C

Sample

1. Nicardipine

2. Verapamil

3. Alprenolol

0 25 50 75 100 min

1

1

1

1

2

2

2

23

3

3

3

N

O

O

NO2

O

ON

OHN

OH

-12-

Basic Drugs




Detection UV 210 nm


Temperature 40 °C

Sample

1. Nicardipine

2. Verapamil

3. Alprenolol

OHN

OH

N

O

O

NO2

O

ON

0.0 25.0 50.0 75.0 100.0 min

1

2

3

-13-

Basic Drugs



Mobile Phase CH3CN/ 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 80 / 20

Detection UV 210 nm


Temperature 40 °C

Sample

1. Nicardipine

2. Verapamil

3. Alprenolol

4. Clomipramine

5. Chlorpromazine

OHN

OH

N

N

Cl

S

N

N

Cl

N

O

O

NO2

O

ON

0 10 20 30 40 min

1

2

3

4

5

-14-

L-Glutamic Acid, GABA

Column : TCI Dual ODS-CX10 Column : TCI Dual ODS-CX15 Column : TCI Dual ODS-CX20 Column : Kaseisorb LC ODS 2000 Column Size 4.6 mmI.D. X 150 mm

Mobile Phase CH3CN / H2O / H3PO4 = 5 / 95 / 0.1

Detection UV 210 nm


Temperature 25 ℃

Sample

1. L-Glutamic Acid

2. GABA (-Aminobutyric Acid)

H2NOH

O

HO OH

O O

NH2

0 10 20 30 40 50 min

1

1

1

1

2

2

2

2

-15-

0 10 20 30 40 50 60 70 80 min

1

1

1

2

2

2,6

3

3

3

4

4

4

5

5

5

6

6

Sulfonic Acids Mobile Phase : CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 50 / 50 Mobile Phase : CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50 Mobile Phase : CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50



Detection UV 254 nm


Temperature 30 ℃

Sample

1. Benzene

2. Toluene

3. Ethylbenzene

4. Benzenesulfonic Acid 5. p-Toluenesulfonic Acid

6. 4-Ethylbenzenesulfonic Acid

SO3HSO3H SO3H

-16-

Sulfonic Acids Mobile Phase : CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 50 / 50

Mobile Phase : CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50

Mobile Phase : CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50



Detection UV 254 nm


Temperature 30 ℃

Sample

1. Benzene

2. Toluene

3. Ethylbenzene



SO3HSO3H SO3H

0.0 5.0 10.0 15.0 20.0 25.0 min

1

1

1

2

2

2

3

3

3

4

4

4

5

5

5

6

6

6

-17-

0.0 5.0 10.0 15.0 20.0 25.0 min

1

1

1

2

2

2

3

3

3

4

4

4

5

5

5

6

6

6

Sulfonic Acids Mobile Phase : CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 50 / 50

Mobile Phase : CH3CN / 50 mmol/L CH3COONH4 (pH 5.0, CH3COOH) = 50 / 50

Mobile Phase : CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50

Column Kaseisorb LC ODS-SAX Super


Detection UV 254 nm


Temperature 30 ℃

Sample

1. Benzene

2. Toluene

3. Ethylbenzene



SO3HSO3H SO3H

-18-





Mobile Phase CH3CN / 30 mol/L CH3COONH4 (pH 4.5, CH3COOH) = 40 / 60

Detection UV 230 nm


Temperature 30 °C

Sample

1. Dopamine

2. Benzonitrile

3. 3,5-Dihydroxybenzoic Acid

HO

HO

NH2 CN

OHHO

C

O

OH

0.0 2.5 5.0 7.5 10.0 12.5 min

1

1

1

2

2

2

3

3

3

-19-

Column : TCI Dual ODS-CX10




Mobile Phase CH3CN / H2O / TFA = 20 / 80 / 0.05

Detection UV 254 nm


Temperature 40 °C

Sample

1. Caffeine

2. L-Phenylephrine

N

N N

N

O

O

NH

OH

HO

H

L-Phenylephrine is not elution.

0.0 5.0 10.0 15.0 min

1

1

1

1

2

2

2

-20-

0.0 5.0 10.0 15.0 min

0

10

20

30

40

50

60

70

mAU

Halogenated Acetic Acids



Mobile Phase CH3CN / 25 mmol/L HCOONH4 (pH 3.5, HCOOH) = 50 / 50

Detection UV 210 nm


Temperature 30 °C

Sample

1. Monochloroacetic Acid

2. Dichloroacetic Acid

3. Trichloroacetic Acid

1

3

2

OH

O

Cl ClOH

O

Cl

ClOH

O

ClCl

-21-

0.0 5.0 10.0 15.0 min

0

100

200

300

400

500

600

700

mAU

Imidazoles



Mobile Phase CH3CN / 50 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80

Detection UV 210 nm


Temperature 40 °C

Sample

1. 2-Methylimidazole 2. 2-Ethyl-4-methylimidazole

1

2

N

NH

NH

N

-22-

Herbicide






Mobile Phase CH3OH / 5 mmol/L KH2PO4 (pH 3.0, H3PO4) = 20 / 80

Detection UV 210 nm


Temperature 40 °C

Sample

Glyphosate

HO

HN P

OH

O O

OH

0 10 20 30 40 50 min

-23-

Histamine



Mobile Phase CH3CN / 100 mmol/L CH3COONH4 = 50 / 50

Detection UV 230 nm


Temperature 25 °C

Sample

Histamine

N

NH

H2N

-24-

Histamine



Mobile Phase CH3CN / 100 mmol/L CH3COONH4 = 50 / 50

Detection UV 230 nm


Temperature 25 °C

Sample

Histamine

N

NH

H2N

-25-

5-Hydroxy-L-tryptophan

Column : TCI Dual ODS-CX10





Detection UV 254 nm


Temperature 25 °C

Sample

5-Hydroxy-L-tryptophan

0.0 2.5 5.0 7.5 10.0 12.5 min

-26-

Metformin

Column : TCI Dual ODS-CX10 Column : TCI Dual ODS-CX15 Column : TCI Dual ODS-CX20 Column : Kaseisorb LC ODS 2000


Mobile Phase CH3CN / 50 mmol/L KH2PO4 (pH 3.0, H3PO4) = 50 / 50

Detection UV 235 nm


Temperature 40 °C

Sample

Metformin

N NH

NH2

NH NH

0 10 20 30 40 50 min

-27-

Metformin and Isobutyl 4-Hydroxybenzoate

Mobile Phase : CH3CN / 50 mmol/L KH2PO4

(pH 3.0, H3PO4) = 50 / 50

Mobile Phase : CH3CN / 50 mmol/L HCOONH4

(pH 3.0, HCOOH) = 50 / 50



Detection UV 235 nm


Temperature 40 °C

Sample

1. Isobutyl 4-Hydroxybenzoate

2. Metformin

0.0 5.0 10.0 15.0 20.0 25.0 min

1

1

2

2

HO

O

O

N NH

NH2

NH NH

-28-

0.0 2.5 5.0 7.5 min

0

10

20

30

40

50

60

70

80

90

mV

2.742

3.729

Levulinic Acid, Methyl Levulinate



Mobile Phase CH3CN / 20 mmol/L K2HPO4 (pH 7.0, H3PO4) = 20 / 80

Detection UV 210 nm


Temperature 25 °C

Sample

1. Levulinic Acid

2. Methyl Levulinate

1 2

OH

O

O

-29-

Melamine

Column : TCI Dual ODS-CX10 Column : TCI Dual ODS-CX15 Column : TCI Dual ODS-CX20



Mobile Phase CH3CN / 20 mmol/L KH2PO4 (pH 3.0, H3PO4) = 40 / 60

Detection UV 230 nm


Temperature 25 °C

Sample

Melamine

NN

N

NH2

NH2H2N

0 10 20 30 40 50 min

-30-

N+

OH OH

OOP

O

NH2

O

P N

OH OH

ON

NN

NH2

O

O-

O-

O

Nicotinic Acid and Derivatives





Detection UV 254 nm


Temperature 30 °C

Sample

1. NAD+

2. Nicotinic Acid

0 5 10 min

1

1

2

2

N

OH

O

-31-

Toluidines

Column

A) TCI Dual ODS-CX10

B) TCI Dual ODS-CX15

C) TCI Dual ODS-CX20



Detection UV 254 nm


Temperature 25 °C

Sample

1. o-Toluidine

2. m-Toluidine

3. p-Toluidine

A) TCI Dual ODS-CX10

C) TCI Dual ODS-CX20

B) TCI Dual ODS-CX15

NH2

NH2NH2

0.0 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 min

1

1

1

2

2

2

3

3

3

-32-

3

min0 2.5 5 10 15 17.5 7.5 12.5

2

3

4

min 0 2 4 6 8 10 12 14 16 18

4

1

Halide Ions Detection : Corona CAD

Detection : UV 210 nm




Detection Corona CAD (Range: 100 pA, Filter: High, Nebulizer: 25 °C), UV 210 nm


Temperature 40 °C

Sample

1. K+

2. Cl-

3. Br-

4. I-

-33-

Alkali Halides




Detection Corona CAD (Range : 100 pA, Filter: High, Nebulizer: 25 °C)


Temperature 40 °C

Sample

1. Cl- 2. Li+ 3. Na+ 4. K+

min0 2.5 5 7.5 10 12.5 15 17.5

1

3

2

4

-34-

Ionic liquid




Detection Corona CAD (Range: 100 pA, Filter: Corona, Nebulizer: 25 °C)


Temperature 40 °C

Sample

1-Butyl-3-methylimidazolium chloride

N

+NCl-

min0 2 4 6 8

Cl- N

+N

-35-

Halide Ions




Detection UV 210 nm


Temperature 40 °C

Sample

1. Br-

2. I-

0.0 5.0 10.0 15.0 min

1 2

-36-

1-Ethyl-3-methylimidazolium salts 1

Column Kaseisorb LC ODS-SAX Super (5μm)

Colum Size 4.6 mmI.D. X 50 mm


Detection Corona CAD


Temperature 40 °C

Sample

1-Ethyl-3-methylimidazolium

(NC)2N-

BF4-

CF3BF3-

CF3SO3-

150000

170000

0 2.5 5 7.5 10 12.5 min

N

N+

(NC)2N-

BF4-

CF3BF3-

CF3SO3-

N

N+

-37-

1-Ethyl-3-methylimidazolium salts 2

Column Kaseisorb LC ODS-SAX Super (5 m)



Detection Corona CAD


Temperature 40 °C

Sample

1-Ethyl-3-methylimidazolium

CF3BF3-

(CF3SO2)2N-

PF6-

150000

170000

0 2.5 5 7.5 10 12.5 min

N

N+

CF3BF3-

(CF3SO2)2N-

PF6-

N

N+

-38-

N NH

NH2

NH NH

Antidiabetic Drugs

Column TCI Dual ODS-CX15 (5 m)


Mobile Phase CH3OH / 20 mmol/L CH3COONH4 (pH 6.78) = 70 / 30

Detection UV 230 nm


Temperature 40 °C

Sample

1. Metformin

2. Buformin

3. Phenformin

Sample Size 1.0 L (1.0 mg/mL)

150000

170000

0 5 10 15 20 25 min

1

2

3

H2N NHNH

NH NH

H2N NHNH

NH NH

-39-

Sialic Acids

Column : TCI Dual ODS-AX10 (5μm)




Detection UV 210 nm


Temperature 30 °C

Sample

1. N-Acetylneuraminic Acid Methyl Ester

2. N-Glycolylneuramic Acid

3. N-Acetylneuraminic Acid Hydrate

4. N-Acetyl-2,3-didehydro-2-deoxyneuraminic Acid

1

←2,3,4

1

2 3

4

0 5 10 min

-40-

Hydrazinobenzoic Acids

Column : TCI Dual ODS-AX10 (5 m)



Mobile Phase CH3CN / 10 mmol/CH3COONH4 (pH 5.0,CH3COOH) = 20 / 80

Detection UV 254 nm


Temperature 40 °C

Sample

1. 4-Hydrazinobenzoic Acid

2. 3-Hydrazinobenzoic Acid

3. Benzoic Acid

3

1

2

←1,2

3

0 min 5 10 15 20

-41-

L-Ascorbic Acid related compounds Column : TCI Dual ODS-AX10 (5 m) Column : TCI Dual ODS-AX20 (5 m)

Column : Kaseisorb LC ODS SAX Super (5 m) Column : Kaseisorb LC ODS 2000



Detection UV 254 nm


Temperature 40 °C

Sample

1. 2-O-α-D-Glucopyranosyl- L-ascorbic Acid

2. L-Ascorbic Acid

3. Erythorbic Acid

3

21

1

2

3

←3

2

1

min0 5 10

←2,3

1→

OH OH

O OHO

OH

-42-

Tranexamic Acid related compounds




Detection UV 210 nm


Temperature 25 °C

Sample

1. 4-Cyanobenzoic Acid

(0.01mg/1.0mL)

2. 4-(Aminomethyl)benzoic Acid (0.01mg/1.0mL)

3. Tranexamic Acid (1.0mg/1.0mL)

Sample Size 1.0L

0 5 10 min

1

2

3

-43-

Creatine related compounds

Column Kaseisorb LC ODS-SAX Super (5 m)



Detection UV 220 nm


Temperature 40 °C

Sample

1. Creatine

2. Phosphocreatine

Sample Size 1.0 L (10mg/mL)

0 5 10 15 min

1

2

-44-

Glutathiones

Column TCI Dual ODS-AX20 (5 m)



Detection UV 230 nm


Temperature 40 °C

Sample

1. Glutathione reduced form (GSH)

2. Glutathione oxidized form (GSSG)

150000

170000

0 5 10 15 20 25 min

1

2

-45-

Organic Acids (Dicarboxylic acids)

Column TCI Dual ODS-AX20 (3 m)


Mobile Phase CH3CN / 10mmol/L KH2PO4 (pH 7.0, H3PO4) = 20 / 80

Detection UV 210 nm


Temperature 40 °C

Sample

1. Tartaric Acid

2. L-Malic Acid

3. Succinic Acid 4. Fumaric Acid

5. Maleic Acid

0 2.5 5.0 7.5 min

1

2

34

5

-46-

Anti-corrosion agent




Detection UV 210 nm


Temperature 30 °C

Sample

1. 4-Methyl-1H-benzotriazole

2. 5-Methyl-1H-benzotriazole

Sample Size 1.0 L（1.0mg/mL mobile phase)

1

2

0 10 20 30 40 min

Methyl-1H-benzotriazole(mixture)

5-Methyl-1H-benzotriazole

-47-

0 2.5 5.0 min

12

Sugar Phosphate




Detection RI


Temperature 40 °C

Sample

1. D-Glucose 6-Phosphate

2. Glucose 1-Phosphate

OOH

OHO

HOOH

P

OO-

O-

-48-

Serotonin and Metabolites



Mobile Phase

A: CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 10 / 90

B: CH3CN / 50 mmol/L HCOONH4 (pH 3.5, HCOOH) = 70 / 30

A to 100 % B in 60 min

Detection UV 254 nm


Temperature 25 °C

Sample

1. 5-Hydroxy-L-tryptophan

2. 5-Hydroxyindole-3-acetic Acid

3.N-Acetylserotonin

4. L-Tryptophan

5. Serotonin

6. Melatonin

NH

HO O

OH

HO

NH

HN

O

NH

NH2

OH

O

NH

HO NH2

0 5 10 15 min

1

2

3

45

6

-49-

Adenosine Phosphates



Mobile Phase

A : CH3CN / 10 mmol/L NH4HCO3 (pH 7.8) = 10 / 90

B : CH3CN / 20 mmol/L NH4HCO3 (pH 7.8) = 30 / 70

0 %B(0-3 min), 0-100 % B (3-13 min), 100 %B(13-20 min)

Detection UV 254 nm


Temperature 40 °C

Sample

1. cAMP

2. AMP

3. ADP

4. ATP

0 5 10 15 min

2

1

3

4

N

NN

N

NH2

O OH

OO

PO

OH

N

NN

N

NH2

OH OH

OOP

O

HO

OH

N

NN

N

NH2

OH OH

OOP

O

O

OH

PHO

O

OH

N

NN

N

NH2

OH OH

OOPO

O

OH

PO

O

OH

PHO

O

OH

-50-

Thyroid hormones

Column TCI Dual ODS-CX15 (5μm)


Mobile Phase

A:CH3CN

B:20 mmol/L HCOONH4(pH 3.0, HCOOH)

80-40%B (0-8min), 40%B (8-15min)

Detection UV 254 nm


Temperature 40 °C

Sample

1. DL-Thyronine

2. 3,5-Diiodo-L-thyronine

3. 3,3',5-Triiodo-L-thyronine (T3) 4. L-Thyroxine (T4)

0 5 10 min

1

2

3

4

-51-

Food additives

Column TCI Dual ODS-AX10 (5μm)


Mobile Phase

A:CH3CN / 5 mmoL/L CH3COONH4 = 5 / 95

B:CH3CN / 50 mmoL/L CH3COONH4 = 70 / 30

20-100%B (0-5min), 100%B (5-20min)

Detection UV 254 nm


Temperature 40 °C

Sample

1. L-Ascorbic Acid (Vitamin C)

2. 2-Phenoxyethanol

3. Dehydroacetic Acid 4. Sorbic Acid

5. Benzoic Acid

0 5 10 15 min

2

1

3

4

5

-52-

N,N-Dimethylsulfamide

Column TCI Dual ODS-CX15 (5μm)


Mobile Phase CH3OH / 20 mmol/L CH3COONH4 (pH 6.88) = 2 / 98

Detection UV 210 nm


Temperature 40 °C

Sample

0 5 10 min

S

O

N NH2O

00_Index01_Sialic Acids02_Organic Acids03_Organic Acids04_Adenosine Phosphates05_Nucleotides06_Nucleotides07_Nucleotides08_Catecholamines09_Amino Acids10_AllantoinD-Panthenol11_Basic Drugs12_Basic Drugs13_Basic Drugs14_GABA15_Sulfonic Acids16_Sulfonic Acids17_Sulfonic Acids18_Dopamine19_Caffeine20_Halogenated Acetic Acid21_Imidazoles22_Herbicide23_Histamine24_Histamine25_5-Hydroxy-L-tryptophan26_Metformin27_Metformin28_Levulinic29_Melamine30_Nicotinic Acid and Derivatives31_Toluidine32_Halide ions33_Alkali halides34_Ionic liquid35_Halide Ions36_1-Ethyl-3-methylimidazolium salts137_1-Ethyl-3-methylimidazolium salts238_Antidiabetic Drugs39_Sialic Acids40_Hydrazinobenzoic Acids41_L-Ascorbic Acid related compounds42_Tranexamic Acid relational compound43_Creatine related compounds44_Glutathiones45_Organic Acids46_Anti-corrosion agent47_Sugar Phosphate48_Serotonin and metabolites49_Adenosine Phosphates50_Thyroid hormones51_Food additives52_N,N-Dimethylsulfamide

cation-exchange anion-exchange chemical name structural … · a : ch3oh / 10 mmol/l k2hpo4 (ph...

Documents