ce421/521 energy and metabolism. bioenergetics thermodynamic considerations thermodynamic...
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CE421/521CE421/521Energy and Metabolism
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BioenergeticsBioenergetics
Thermodynamic considerations Thermodynamic relationships govern whether a
reaction can occur Simply because a relationship is
thermodynamically possible still may not occur Could be activation energy required Biochemical reactions require specific enzymes Genetic potential required for production of specific
enzymes
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Gibbs Free EnergyGibbs Free Energy
STHG Where ΔG is the change in Gibbs free energyΔH is the change in enthalpy and ΔS is the change in entropy for a closed system at constant pressure
For a reaction to proceed, the entropy of the system must increase, i.e., ΔG must be negative
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Gibbs Free Energy (cont’d)Gibbs Free Energy (cont’d)
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Gibbs Free Energy (cont’d)Gibbs Free Energy (cont’d)
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Gibbs Free Energy (cont’d)Gibbs Free Energy (cont’d)
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Gibbs Free Energy (cont’d)Gibbs Free Energy (cont’d)
ΔG° for elements is zero Just because ΔG° is negative does not
necessarily mean the reaction will proceed Relationship of ΔG° is valid for equilibrium
conditions (says nothing about whether reaction will proceed)
Thermodynamic equilibrium (nothing to say about rate of reaction – kinetics)
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Oxidation ReductionOxidation Reduction
Another measure of the energy contained in a compound is its oxidation state Oxidation is the loss of electrons (often
associated with dehydrogenation) Reduction is the gain of electrons (often
associated with hydrogenation)
The carbon in CH4 is completely reduced and has an oxidation state of -4
The carbon in CO2 is completely oxidized
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Oxidation reduction reactionsOxidation reduction reactions
In biochemical reactions there are electron d________ and electron a__________
In general the electron donor is the energy source
The electron acceptor is the last step in the electron transport system (ETS) the terminal electron acceptor
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ThOD, COD, and BODThOD, COD, and BOD
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Microbial Metabolism
Enzymesp______ – specific for a particular
m_______________ (substrate)c______________ of biochemical reactions,
but do not get consumed in the reactionc__________________ applications exist:
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Enzymes Cont’dEnzymes Cont’d
some non-p_____________ molecules may be involved in enzyme catalyzed reactions: co-factors or co-enzymes (e.g.,
nicotinamide adenine dinucleotide, NAD, NADH, also FAD, FADH)
may also act as e____________ carriers
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EnzymesEnzymes
Six categories of enzymes:
1. oxidoreductases: involved in o____________ reduction reactions
2. transferases: transfer of constituents from one c________________ to another
3. hydrolases: responsible for h___________ of carbohydrates, proteins, and lipids
4. lyases: catalyze the a____________ or removal of constituents
5. isomerases: i____________ formation6. ligases: join m______________, p____________
formation
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KineticsKinetics
Enzyme Kinetics enzymes are “catalysts” in biodegradation and metabolism
S + E ➔ ES ➔ P + ES = substrateE = enzymeES = enzyme substrate complex
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Michaelis - MentonMichaelis - Menton
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Michaelis - MentonMichaelis - Menton
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Michaelis-Menton vs MonodMichaelis-Menton vs Monod
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Lineaweaver-Burke Example Lineaweaver-Burke Example
V, mol/L min S, mol/L
0.00064 0.01
0.00058 0.008
0.000479 0.006
0.00038 0.004
0.000219 0.002
Calculate vmax and Km for the following data:
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Lineaweaver-Burke Example Lineaweaver-Burke Example
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Microbial Growth Kinetics
Procaryotic cells divide by b________ fission: simple c_________ of DNA and cell division
growth rate = increase in n__________ of microorganisms or increase in microbial m___
time required for microbial population to d__________ = generation time (doubling time) during unlimited growth conditions
b____________ versus continuous culture growth curve:
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Growth CurveGrowth Curve
0 0.1 0.2 0.3 0.4
T im e, h
0
0.4
0.8
1.2
1.6
2
Am
ou
nts
of S
ub
stra
te, B
iom
ass,
an
d O
xyg
en
, mg
CO
D L
-1
S ubstra te D isappearance
O xygen C onsum ed
B iom ass G enerated
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Growth Curve (log scale)Growth Curve (log scale)
stationary phase, g________ = decay death phase – how to distinguish bacterial
d___________ versus bacterial d_________?
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Continuous CultureContinuous Culture
m________ b______________ on substrate:
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