Copyright © 2012 Abcam, All Rights Reserved.Produced in collaboration with Sarah Barnes from the laboratory of Dr Sue Ozanne, Institute of Metabolic Science (IMS), University of Cambridge.

Cell ageing and metabolism

Discover more at abcam.com/metabolism

* Studies have shown increased lifespanwith over expression.

† Original paper demonstrated a 30%increase in lifespan with over expressingSirt1, however out breeding reduced thisto a more modest 10%.

Antioxidants Catalase*, Gpx1, Gsr, Hsp70*,Sod1*, Sod2*, Sod3, Prdx1,Prdx2, Prdx3, Txn1

Senescencep53, p21, p16

DNA damageGadd45g, Ku70, Ku80, Sirt6*

Master regulatorsSirt1†, Bach1, Nrf2

Telomere MaintenancePot1, Ku80, Trf1, Trf2

Product Applications/Tests Species Reactivity Product Code Product Applications/Tests Species Reactivity Product Code

410_12_MBS

Cell ageing and metabolism products from Abcam

Discover more at abcam.com/metabolism

Antioxidants | Antibodies

Catalase IHC-Fr, IHC-P, ICC, WB, IP, ELISA, ICC/IF Ms, Rat, Shp, Chicken, Cow, Hu ab1877

Glutathione Peroxidase 1 IHC-P, WB, ICC/IF, IHC-Fr Ms, Rat, Cow, Hu ab22604

Glutathione Reductase [EPR7238] WB, Flow Cyt Ms, Rat, Hu ab124995

Hsp70 antibody [C92F3A-5] Electron Microscopy, WB, IP, ELISA, ICC/IF, Ms, Rat, Sheep, Rab, Chicken, Guinea pig,

Flow Cyt, IHC-Fr, IHC-P Hamster, Cow, Dog, Hu, Pig, C.elegans, Carp,

Fruit fly (Drosophila melanogaster), Monkey ab47455

Superoxide Dismutase 1 ICC/IF, IHC-Fr, WB, IP, ELISA, IHC-P Ms, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Hu

Pig, X.laevis, Fruit fly (Drosophila melanogaster),

Monkey, African Green Monkey ab13498

SOD2 [9E2BD2] ICC/IF, IHC-P, IP, In-Cell ELISA, Flow Cyt Ms, Rat, Cow, Hu ab110300

Superoxide Dismutase 3 IHC-Fr, IHC-P, WB Hu ab21974

Peroxiredoxin 1 ICC/IF, IHC-P, WB, ICC Ms, Rat, Hu, African Green Monkey ab15571

Peroxiredoxin 2 antibody [EPR5154] WB, IHC-P, ICC/IF Ms, Rat, Hu ab109367

Peroxiredoxin 3 antibody [EPR8115] WB, IP, IHC-P, ICC, Flow Cyt Hu ab128953

Thioredoxin WB, ICC/IF, IHC-P Ms, Hu ab26320

Antioxidants | Kits

Catalase Human ELISA Kit 1 x 96 Hu, Rat, ms ab123456

Catalase Specific Activity Assay Kit 1 x 96 Ms, Rat, Hu ab118184

Glutathione Peroxidase Assay Kit 1 x 100 Cell culture supernatant, Urine, Serum, Plasma,

Platelets, Other biological fluids, Tissue Extracts ab102530

Glutathione Reductase (GR) Assay Kit 1 x 200 Cell culture supernatant, Urine, Serum, Plasma,

Other biological fluids, Tissue Extracts, Cell Lysate ab83461

Superoxide Dismutase Activity Assay Kit (Colorimetric) 1 x 100 Urine, Serum, Plasma, Other biological fluids,

Tissue Extracts, Cell Lysate, Cell culture media ab65354

Thioredoxin (TrxR) Reductase Assay Kit 1 x 100 Cell culture supernatant, Urine, Serum, Plasma,

Other biological fluids, Tissue Extracts ab83463

Total Antioxidant Capacity Assay Kit 1x100 Lysates, plasma, serum, urine ab65329

NAD/NADH Assay Kit 1x100 Lysates, plasma, serum, urine ab65348

NADP/NADPH Assay Kit 1x100 Lysates, plasma, serum, urine ab65349

Senescence | Antibodies

p53 [PAb 240] Flow Cyt, IHC-Fr, IP, ICC/IF, WB, IHC-P Ms, Rat, Dog, Hu ab26

p21 IHC-Fr, IP, WB, ICC/IF, ICC, Flow Cyt Ms, Rat, Dog, Hu ab7960

p16 ARC ICC/IF, Flow Cyt, IP, WB, IHC-P Ms, Rat, Dog, Hu ab51243

Senescence | Kits

p53 Human ELISA Kit 1 x 96 Hu ab117995

p53 Phospho S46 Human ELISA Kit 1 x 96 Hu ab124532

Senescence | Kits (continued)

p53 Phospho S392 Human ELISA Kit 1 x 96 Hu ab124533

p53 Total + pSer46 Human In-Cell ELISA Kit (IR) 1 x 96 Hu ab128570

p53 Total + pSer392 Human In-Cell ELISA Kit (IR) 1 x 96 Hu ab128571

Senescence Detection Kit 250 Cell culture, tissue ab65351

DNA damage | Antibodies

GADD45G WB, Flow Cyt Hu

Ku70 [N3H10] IF, IHC-P, IP, WB, ICC/IF Ms, Hu, Monkey ab3114

Ku80 [5C5] WB, ELISA, IHC-P, Flow Cyt, ICC/IF Ms, Hu ab119935

Ku70 + Ku80 [162] ICC/IF, Flow Cyt, IHC-P, IP Hu, X.laevis, Monkey ab3108

SIRT6 WB, IHC-P Hu ab118487

Anti-GAPDH + H2A.X(S139) +cleaved-PARP

antibody [3E8, 9F3 and 4B5] WB Hu ab131385

DNA damage | Kits

DNA Damage - AP sites - Assay Kit (Colorimetric) 25 Purified damaged DNA ab65353

In Situ Direct DNA Fragmentation Assay Kit

(TUNEL assay) 50 Adherent and suspension cells ab66108

In Situ BrdU-Red Fragmentation Assay Kit

(TUNEL assay) 60 Tissue, adherent cells ab66110

Master regulators | Antibodies

SIRT1 [E104] IHC-Fr, WB, ICC, IP, IHC-P Hu ab32441

BACH1/BRIP1 WB Hu ab7288

Nrf2 IHC-P, ICC/IF, IHC-Fr, WB Ms, Rat, Hu ab31163

Master regulators | Kits

SIRT1 Human ELISA Kit 1 x 96 Tissue Extracts, Cell Lysate ab123457

SIRT1 pSer47 Human ELISA Kit 1 x 96 Hu ab135392

SIRT1 Total and pSer47 Human In-Cell ELISA Kit (IR) 1 x 96 Hu ab135394

Telomere Maintenance | Antibodies

POT1 WB, ICC/IF Ms, Hu ab21382

Trf1 ChIP Grade ChIP, WB, ICC/IF, IP Ms, Hu ab1423

Trf2 IHC-P, WB, ICC, IP, ICC/IF, Flow Cyt Hu ab13579

Telomere Maintenance | Kits

EpiSeeker ChIP Kit - One Step 48 / 96 Mammalian DNA ab11713