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  • 8/17/2019 Cell Biology Test 3

    1/11

    B[OL265 Exam

    3

    )

    The distinct characteristics

    of different

    cell tlpes in

    a multicellular

    organism

    result mainly

    from

    the

    diflerential regulation

    of the

    _.

    A) transcription

    of

    genes

    transcribed

    by

    RNA

    polymerase

    II.

    B) transcriptionofhousekeepinggenes.

    C)

    proteins

    that

    dtectly

    bind

    the

    TATA

    box of eukaryotic

    genes.

    D), replication

    ofspecific

    genes.

    The human

    genome

    encodes

    about 21,000

    protein-coding

    genes.

    Approximately

    how many

    such

    genes

    does

    the typical differentiated

    human cell

    express at any one

    time?

    A)

    between 5000 and 15,000

    B) less

    than 2100

    C)

    between 18,900

    and

    21,000-at

    least

    90% of

    the

    genes

    D) 21,0OG-all of them

    Which

    of the following is not

    a

    good

    example

    of

    a

    housekeeping

    protein?

    A)

    hemoglobin

    B)

    ATP

    synthase

    C) DNA

    repair enzymes

    D)

    histones

    Which of the following

    statements

    about differentiated

    cells is

    true?

    A)

    Some of

    the

    proteins

    found

    in

    differentiated

    cells

    are

    found in

    all cells

    of a multicellular

    organism.

    B)

    Cells

    of distinct t)?es

    express nonoverlapping

    sets

    of transcription factors.

    C) Once a cell

    has

    differentiated, it

    can

    no longer

    change its

    gene

    expression.

    D)

    Once a cell has differentiated,

    it will

    no

    longer

    need

    to transcribe

    RNA.

    Investigators

    performed

    nuclear

    transplant experiments

    to determine

    whether

    DNA

    is altered

    irreversibly

    during

    development.

    Which

    of

    the

    following

    statements about

    these

    experiments

    is

    true?

    A)

    Although nuclear

    transplantation

    has been successful

    in

    producing

    embryos

    in

    some mammals

    with

    the use

    of

    foster

    mothers,

    evidence

    of DNA

    alterations

    during

    differentiation

    has not

    been

    obtained

    for

    plants.

    B)

    Because

    the donor nucleus

    is

    taken

    from

    an adult

    animal, the

    chromosomes

    from

    the nucleus

    urust

    undergo recombination

    with

    the

    DNA

    in

    the

    egg for successful

    development

    to occur.

    C) The

    embryo that develops

    from

    the nuclear

    transplant

    experiment

    is

    genetically

    identical

    to the

    donor

    of the nucleus.

    D) The meiotic

    spindle

    of the

    egg must interact

    with

    the chromosomes

    of the injected

    nuclei

    for

    successful nuclear

    transplantation

    to

    occur.

    J.

    4.

    5.

    Page

    I

  • 8/17/2019 Cell Biology Test 3

    2/11

    Use the

    following

    to answer

    questions

    6-9:

    You

    are

    interested in examining

    the

    Ps/gene.

    It is known

    that Psf is

    normally

    produced

    when cells

    are

    exposed

    to high levels

    of both

    calcium

    (Ca*)

    and magnesium

    (Mg'*).

    MetA, MetB,

    and MetC

    are

    important for

    binding to the

    promoter

    of the Ps/gene

    and are

    involved in

    regulating

    its

    transcription.

    MetA

    binds to

    the

    "A"

    site in the

    promoter

    region, MetB to

    the

    "B"

    site, and

    MetC to the

    "C"

    site.

    You

    create

    binding-site mutations in

    the A, B,

    and C sites and observe

    what

    happens

    to transcription of

    the

    Py'gene.

    Your results

    are

    summarized in Table

    Q8-17.

    Transcription

    in the

    presence

    of Ca2* andlor

    Mgz+

    No Ca2*

    Binding

    sites

    present

    or Mgz*

    with with

    with

    ca2+

    Ca2* Mg'*

    and Mg2*

    4

    u"d

    A,

    B,

    and

    C

    A only

    B only

    C only

    A and

    B only

    A and C only

    B and C only

    For this table:

    -

    -

    no

    transcription

    of Pst

    +

    =

    low level

    of transcription of

    Psf,

    and

    ++

    =

    high levels of transcription

    of Psf.

    Table

    Q8-17

    Which of the following

    proteins

    are

    likely

    to

    act

    as

    gene

    activators?

    A)

    MetB

    only

    B) MetC only

    C) Both MetA and MetC

    D) MetA

    only

    Which

    of

    the

    following

    proteins

    are

    likely

    to act

    as gene repressors?

    A) MetB

    only

    B)

    Both

    MetA and MetC

    C)

    MetA

    only

    D)

    MetC only

    Which

    kanscription

    factors

    are

    normally

    bound to

    the

    Ps/promoter

    in

    the

    presence

    of Mg2*

    only?

    A) MetA, MetB,

    andMetC

    B)

    none

    C) MetAonly

    D) MetA

    and

    Met B

    Which

    transcription factors

    are normally

    bound

    to

    the

    Ps/promoter

    in

    the

    presence

    of both Mg2*

    and

    Ca2*?

    A) MetA, MetB,

    andMetC

    B) MetAandMetC

    C)

    MetAandMetB

    D) MetB

    andMetC

    .t'

    4oI\'

    ++

    6.

    7.

    8.

    9.

    Page2

  • 8/17/2019 Cell Biology Test 3

    3/11

    Use the following

    to

    answer

    questions

    l0-13:

    You

    are

    interested

    in

    the

    regulation

    of

    gene

    Q.

    Proteins

    G, H,

    and

    J

    are

    proteins

    that

    are important

    for

    regulating

    gene

    e,

    and

    bind to

    its

    promoter

    region

    in

    a

    sequence-specific

    fashion.

    Proteins

    G and H

    both

    bind to site

    "A"

    but cannot

    bind

    to site;.A"

    ai

    the

    same

    time.

    Protein J

    binds to site

    "B"

    on

    the

    promoter.

    The

    promoter

    region

    is

    diagrammed

    in Figure

    Q8-29.

    Gt+

    m*ftlAaf

    gun**

    Figure

    Q8-29

    You

    develop

    a

    cell-free transcriptional

    system to study

    the effects

    of

    proteins

    G, H,

    and

    J

    on the transcription

    of

    gene

    e.

    Using

    this

    system,

    you

    can

    examine the

    effects of

    adding these

    proteins

    to

    the

    transcriptional

    system in

    equal amounts

    and

    measuring

    how much

    gene

    Q

    is

    produced.

    When

    you

    add these

    proteins

    to

    the system,

    you get

    the results

    shown

    in

    Table

    Q8-29.

    e $efifn*nt

    r,x*mhef

    rBflrrlsttr

    pro**ift

    *ddsd

    I

    *nHH*

    fi

    ll I

    laede?

    *qf

    ffi

    ?4+*y#

    s

    Table

    Q8-29

    Ws

    y**

    10.

    ll.

    t2.

    Which proteins

    are

    likely

    to

    act

    as

    gene

    activators?

    A)H

    B)J

    C) both H

    and

    J

    D)G

    Which

    proteins

    are likely

    to

    act

    as

    gene

    repressors?

    A)J

    B)G

    C)

    both H

    and

    J

    D)H

    Your

    colleague

    looks

    at

    your

    data

    above

    and

    predicts

    that

    protein

    G

    will

    bind more

    strongly

    to

    the DNA

    at

    site A,

    compared

    to

    protein

    H.

    Which

    experiment

    above is

    critical

    for

    this

    prediction?

    A)

    #6

    B) #2

    c)

    #3

    D)

    #5

    Page

    3

  • 8/17/2019 Cell Biology Test 3

    4/11

    I3.

    t4.

    Which

    proteins

    do

    you

    predict

    are

    bound

    to

    the

    promoter

    in experiment

    #8?

    A)

    onlyG

    andH

    B)

    only J

    C)

    only

    H

    and

    J

    D)

    only

    G and

    J

    How are most

    eukaryotic

    transcription

    regulators

    able

    to affect transcription

    when

    their

    binding

    sites

    are

    far from

    the

    promoter?

    A)

    .

    by

    looping

    out the

    intervening

    DNA

    between

    their

    binding

    site and

    the

    promoter

    B)

    by

    unwinding

    the DNA

    between

    their

    binding

    site and

    the

    promoter

    C) by attracting

    RNA

    polymerase

    and

    modifying

    it before

    it

    can

    bind to

    the

    promoter

    D) by

    binding to their

    binding

    site and

    sliding

    to

    the

    site of RNA

    polymerase

    assembly

    The

    expression

    of

    the

    BRFI

    gene

    in

    mice is

    normally

    quite

    low,

    but mutations

    in

    a

    gene

    called.BRF2

    lead,

    to increased

    expression

    of BRFl.

    You

    have

    a

    hunch

    that

    nucleosomes

    are involved

    in

    the regulation

    of

    ,BRF1

    expression and

    so

    you

    investigate

    the

    position

    of

    nucleosomes

    over

    the

    TATA

    box

    ofBRFl in

    normal mice

    and

    in mice

    that lack

    either

    the BRF2

    protein

    (BRF2)

    or

    part

    of histone

    H4

    (HHF-)

    (histone

    H4

    is encoded

    by the

    HHF

    gene).

    Table

    Q8-37

    summarizes your

    results.

    A normal

    functional

    gene

    is

    indicated

    by a

    plus

    sign

    (+).

    Mouse

    Nucleosome

    positioning

    Relative

    level

    of BRFI

    mRNA

    BRFZ+

    HHF+

    specific

    pattern

    BRF2-

    HHF+

    randorn

    100

    BRF?+ HHF-

    random

    BRF2'HHF-

    randgm

    100

    Table

    Q8-37

    Which of the

    following

    conclusions

    cannotbe

    drawn

    from

    your

    data?

    Explain

    your

    answer.

    A)

    The

    part

    of histone

    H4 missing

    nHHf-

    mice is not

    required

    for

    the

    formation

    of nucleosomes.

    B)

    The specific pattem

    of nucleosome positioning

    over

    the

    BXFI

    upstream

    region

    is required

    for BRFI

    repression.

    C)

    BRF2

    is requted

    for

    the repression

    of BRFI.

    D)

    BRF2

    is required

    for

    the

    specific

    pattern

    of nucleosome positions

    over

    the

    BRFI

    upstream

    region.

    Combinatorial

    control

    of

    gene

    expression

    _.

    A) involves groups

    of

    transcriptional

    regulators

    working

    together

    to deiermine

    the

    expression

    of a

    gene.

    involves

    only the

    use

    ofgene

    activators

    used

    together

    to regulate

    genes

    appropriately.

    is

    seen

    only

    when

    genes

    are

    arranged

    in

    operons.

    involves

    every

    gene

    using

    a

    different

    combination

    of transcriptional

    regulators

    for

    its

    proper

    expression.

    17.

    The

    extent

    of complementarity

    of

    a

    miRNA

    with its

    target

    mRNA

    determines

    whether

    the

    mRNA

    will

    be

    tansported to

    the

    nucleus.

    whether

    the

    miRNA

    synthesizes

    a

    complementary

    strand.

    whether

    the mRNA

    will

    be immediately

    degraded

    or

    whether

    the rrRNA

    will fust

    be transported

    elsewhere in

    the

    cell before

    degradation.

    whether

    RISC

    is

    degraded.

    15.

    16.

    B)

    c)

    D)

    A)

    B)

    c)

    D)

    Page

    4

  • 8/17/2019 Cell Biology Test 3

    5/11

    The owners of

    a

    local bakery ask for

    your

    help in improving

    a

    special

    yeast

    strain they use

    to

    make

    bread.

    They would like

    you

    to help

    them

    design

    experiments using

    RNA interference

    to

    turn off

    genes,

    to allow

    them to

    test their hlpothesis that certain

    genes

    are important for the

    good

    flavors found in

    their bread.

    Of

    the components

    in

    the

    following list, which

    is the most

    important to

    check

    for in

    this

    yeast

    strain

    if

    you'd

    like this

    project

    to

    succeed?

    A) the

    presence

    of

    genes

    in the

    genome

    that code

    for RISC

    proteins

    B)

    the

    presence

    of single-standed siRNAs

    within

    the cell

    C)

    the

    presence

    of

    foreign

    double-stranded

    RNA

    D)

    .

    the presence

    of

    miRNA

    genes

    in

    the genome

    You

    have

    a

    circular

    plasmid

    that

    can

    be

    cut by

    the restriction nuclease HindIII,

    as

    diagrammed in Figure

    Qr0-4.

    Figure

    Q10-4

    If

    you

    were

    to

    cut

    this circular

    piece

    of DNA with HindIII, which of the answers below

    best

    predicts

    what

    you

    would

    get?

    A)

    two circular

    pieces

    of DNA

    B)

    two semicircular

    pieces

    of

    DNA

    C)

    t',vo

    linqar

    pieces

    of

    DNA

    D)

    one linearpiece of

    DNA

    You have a

    piece

    of circular

    DNA that

    can

    be cut by the

    restriction

    nucleases

    XhoI and SmaI, as indicated

    Figure

    Q10-5

    If

    you

    were

    to cut this circular

    piece

    of

    DNA with

    both

    XhoI

    and SmaI,

    how

    many fragments of

    DNA

    would

    you

    endup with?

    A)2

    B)4

    c)1

    D)3

    Page

    5

  • 8/17/2019 Cell Biology Test 3

    6/11

    Notl

    21.

    You

    have

    a

    piece

    of

    circular

    DNA

    that

    can

    be cut

    by

    the

    reshiction

    nucleases

    EcoR[,

    HindIII,

    and NotI,

    as

    indicated

    in

    Figure

    Ql0-6.

    EcoRl

    Hindlll

    Figure

    Q10-6

    Which of

    the following

    statements

    isfalse?

    A)

    A

    piece

    of DNA

    that

    cannot

    be cut

    by EcoRI

    will

    be obtained

    by cutting

    this

    DNA

    with

    both

    NotI

    and

    HindIII.

    B)

    Two DNA

    fragments

    that cannot

    be

    cut by

    HindIII

    will

    be

    obtained

    when

    this DNA

    is

    cut

    by EcoRI

    and

    NotI.

    C) Two

    DNA

    fragments

    of unequal

    size will

    be created

    when

    this DNA

    is

    cut

    by both

    HindIII and

    EcoN.

    D)

    One

    piece

    of

    DNA

    will

    be

    obtained

    when

    this

    DNA

    is cut

    by NotI.

    Which

    of the following

    statements

    about

    gel-transfer

    hybridization (or

    Southern

    blotting)

    isfalse?

    A) A

    labeled

    DNA

    probe

    binds to

    the DNA

    by hybridization.

    B)

    The

    DNA

    that is

    separated

    on

    a

    gel

    is

    not labeled.

    c) In this

    technique,

    single-stranded

    DNA is

    separated

    by electrophoresis.

    D)

    This

    technique

    involves

    the

    transfer

    of DNA

    molecules

    from

    gel

    onto

    nitrocellulose

    paper

    or nylon

    paper.

    DNA ligase

    is

    an enzyme

    used when

    making

    recombinant

    DNA

    molecules

    in

    the lab.

    In

    what

    normal

    cellular

    process

    is

    DNA

    ligase

    involved?

    A) transcription

    B)

    transformation

    C) DNA

    replication

    D)

    none,

    it is

    only found

    in

    virally

    infected

    cells

    Q10-19

    shows

    the cleavage

    sites

    of

    several

    reskiction

    nucleases.

    22.

    23.

    24,

    5',

    3',

    s'

    otoocrr

    3'

    3,rEEEh

    s,

    5,

    ACAT"I,

    ,,

    3'

    r[crACA

    5'

    Figure

    Q10-19

    You

    cut a vector

    using

    the PciI

    restriction

    nuclease.

    Which

    of the

    following

    restriction

    nucleases

    will

    generate

    a

    fragment

    that

    can

    be ligated

    into

    this

    cut

    vector

    with

    the

    additio;

    of

    only

    ligase

    and

    ATp?

    A)

    NspV

    B)

    HindIII

    C)

    NcoI

    D)

    MmeI

    t3'

    5'ccAlTGG

    3'

    5'

    3'GGTIACC

    5'

    5',

    3',

    Page

    6

  • 8/17/2019 Cell Biology Test 3

    7/11

    Figure

    Q10-20

    depicts

    a

    strategyby

    which

    a

    DNA

    fragment

    produced

    by cutting

    with the

    EcoRI

    restriction nuclease

    can

    be

    joined

    to a

    DNA

    fragment

    produced

    by

    cutting

    DNA

    with the HaeIII

    restriction nuclease.

    + ligase

    .ffi

    -T*

    .H,H'

    , $_\EtiF-

    -+

    + deoxyribo-

    nucleotides

    -GAATTI

    .wffi$#.&_

    *'CfTAA.

    tr"

    ;::gip/;:

    ; i#, ;

    +

    polymerase

    Figure

    Q10-20

    Note that cutting DNA with EcoRI

    produces

    a

    staggered end,

    whereas cutting

    DNA

    with HaeIII

    produces

    a blunt end. Why must

    polymerase

    be added in this reaction?

    A)

    Without

    polymerase,

    there will not

    be enough

    energy

    for

    the reaction to

    proceed.

    B) Polymerase

    will

    add

    nucleotides

    to

    the

    end

    produced

    by the

    HaeIII

    restriction nuclease.

    C) Polymerase will fill in

    the staggered

    end to create

    a

    blunt end.

    D)

    Polymerase is

    needed

    to

    seal

    nicks in

    the

    DNA

    backbone.

    DNA can

    be infoduced into

    bacteria by

    a

    mechanism

    called

    A)

    ligation.

    B)

    replication.

    C) transformation.

    D) transcription.

    A

    plasmid

    _.

    A) is

    a

    single-stranded

    circular

    DNA

    molecule

    that

    can

    undergo horizontal

    transfer

    among

    bacteria.

    B)

    always becomes

    part

    of

    the

    bacterial

    chromosome

    during transformation.

    C)

    can

    confer antibiotic resistance

    to a

    bacterium.

    D)

    is

    a

    tool

    designed

    in

    the lab and never

    found in naturally occurring

    bacteria.

    Which of the following

    statements

    about DNA

    librarie

    s

    is

    true?

    A)

    Individual

    bacteria that have

    taken up most

    of the library

    DNA

    are

    selected for

    during

    the

    construction

    of

    a

    DNA library.

    The library

    DNA within the

    bacteria

    will

    only

    be replicated

    when it hybridizes

    to

    a

    DNA

    probe.

    By

    placing

    the

    library DNA

    into

    bacteria,

    the

    bacteria can

    be used to

    amplifr

    the

    desired DNA

    fragments

    from

    the DNA library

    Production

    of

    a

    DNA library

    involves

    the direct insertion

    of

    short

    DNA

    fragments

    into

    bacteria

    through transformation.

    Which of

    the

    following

    statements

    about genomic

    DNA

    libraries isfalse?

    A) The larger

    the size

    of

    the fragments

    used

    to make

    the library,

    the

    more

    difficult it

    will be

    to find

    your gene

    ofinterest

    once

    you

    have identified

    a

    clone that hybridizes

    to

    your

    probe.

    B) The

    larger

    the

    genome

    of the

    organism from

    which

    a

    library

    is derived,

    the larger

    the fragments

    inserted into

    the vector

    will tend to

    be.

    C)

    The

    smaller the

    gene

    you

    are

    seeking,

    the more

    likely

    it

    is that

    the

    gene

    will be found

    on a single

    clone.

    D)

    The larger the

    size of the fragments

    used

    to make

    the library,

    the fewer

    colonies

    you

    will

    have

    to

    examine

    to find a

    clone that hybridizes

    to

    your

    probe.

    B)

    c)

    D)

    PageT

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    30.

    Why

    is

    an excess

    of

    normal

    deoxyribonucleoside

    triphosphate

    molecules

    (dNTPs)

    needed

    during

    dideoxy

    sequencing?

    A)

    dNTPs

    are

    consumed

    as

    energy

    to

    fuel the

    sequencing

    reactions.

    B)

    When dNTP

    levels

    are

    too

    low, there

    will be

    very

    few chain-termination

    events.

    C)

    The dNTPs

    can

    hybridize

    to the

    fragment

    to

    be sequenced

    and serve

    as

    primers for DNA

    polymerase.

    D)

    bN,t

    potymerase

    uses

    the

    dNTPs

    to synthesize

    a DNA

    molecule

    complementary

    to

    the

    molecule

    being

    sequenced.

    you

    create

    a

    recombinant

    DNA

    molecule

    that

    fuses

    the coding

    sequence

    of

    green

    fluorescent

    protein

    to

    the

    regulatory

    DNA

    sequences

    that

    control

    the

    expression

    of

    your

    favorite

    genes.

    Which

    of the

    following

    pieces of

    information

    can

    you

    NOIgain

    by examining

    the expressiol

    of

    this

    reporter

    gene?

    A)

    the cell

    in which

    the

    protein

    encoded

    by this

    gene

    is expressed

    B)

    when,

    during

    an

    organism's

    development,

    this

    gene

    is expressed

    C)

    the

    tissue

    where

    the

    protein

    encoded

    by

    this

    gene

    is expressed

    D)

    the specific

    location

    within

    the

    cell

    of the

    protein encoded

    by

    this

    gene

    In

    the

    photosynthetic

    archaeat

    Halobacterium

    halobium,

    a

    membrane

    transport

    protein

    called

    bacteriorhodopsin

    captures

    "r"rgy

    tom

    suntigfit

    and

    uses

    it

    to

    pump

    protons

    out

    ofthe

    cell.

    The

    resulting

    proton

    gradient

    serves

    as

    an

    energy

    store

    that

    can

    lulter

    be tapped to generate

    ATP.

    Which

    statement

    best

    describes

    how bacteriorhodopsin

    operates?

    A)

    The absorption

    of sunlight

    triggers

    a contraction

    ofthe

    b barrel

    that

    acts as the

    protein's

    central

    channel,

    squeezing

    a

    proton

    out

    of the

    cell.

    B)

    Th;

    absorption

    of

    sunlight

    triggers

    a

    shift

    in

    the

    conformation

    of the

    protein's

    seven,

    membrane

    spanning

    a

    helices,

    allowing

    a

    proton

    to

    leave the

    cell'

    C)

    The

    absorption

    of sunlight

    triggers

    a

    restructuring

    of

    bacteriorhodopsin's

    otherwise

    unstructured

    core

    to

    forrn the

    channel

    through

    which

    a

    proton

    can

    exit the

    cell.

    D)

    The absorption

    of

    sunlight

    triggers

    the activation

    of an

    enzyme

    that

    generates

    ATP.

    Insulin

    is a small

    protein

    that

    regulates

    blood

    sugar

    level

    and

    is

    given

    to

    patients

    who suffer

    from

    diabetes.

    Many

    years

    ago,

    diabetics

    were

    given

    insulin

    that

    had

    been

    purified

    from

    pig pancreas. Once

    recombinant

    DNA

    techniques

    became available,

    the

    DNA encoding

    insulin

    could

    be

    placed into an

    expression

    vector

    and

    insulin

    could

    be

    produced

    in

    bacteria.

    Which

    of the

    followngis

    NOT areason

    why

    purifying insulin

    from

    bacteria

    is

    a

    better

    way

    to

    produce insulin for

    diabetics than

    using

    insulin

    purified

    from

    a

    pig

    pancreas.

    A) Insulin

    made

    from a bacterial

    culture

    and

    then

    purified will be

    free of any

    possible

    contaminating

    viruses

    that

    pigs

    (and

    any other

    animals)

    harbor. Since

    pigs

    are

    more closely

    related to

    people

    than

    bacteria

    are,

    their

    viruses

    are more

    likely

    to be

    harmful

    to

    people

    than

    are

    viruses

    that

    might

    infect

    bacteria.

    B)

    Insulin

    can

    be

    easily

    produced in large

    quantities

    from

    cells carrying

    the

    cloned

    DNA

    sequence.

    C)

    The

    pig

    protein

    has

    slight

    amino

    acid

    differences

    compared

    to

    the

    human

    protein,

    so

    human

    insulin

    produced

    by

    bacteria

    will

    work better

    in

    people.

    D)

    the

    creation

    of

    transgenic

    pigs

    that

    expressed

    insulin was

    very expensive

    compared

    to

    the cost of

    creating

    bacteria

    that expressed

    insulin.

    :,

    Which

    of

    the following

    phenomena will be observed

    if a

    cell's

    membrane

    is

    pierced?

    A)

    the membrane collapses

    B) a tear

    is formed

    C)

    the

    membrane

    expands

    D)

    the membrane

    reseals

    31.

    32.

    JJ.

    34.

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    38.

    39.

    The

    plasma

    membrane

    serves

    many

    functions, many

    of which

    depend on the

    presence

    of

    specialized

    membrane

    proteins.

    Which of the following roles

    of the

    plasma

    membrane

    could

    still occur

    if

    the

    bilayer

    were lacking

    these

    proteins?

    A) selectivepermeability

    B) import/export

    of

    molecules

    C)

    intercellularcommunication

    D)

    cellular movement

    Which of the following membrane

    lipids does not

    contain

    a fatty

    acid tail?

    A)

    cholesterol

    B)

    phosphatidylserine

    C)

    phosphatidylcholine

    D) a

    glycolipid

    Forrnation

    of

    a

    lipid bilayer

    is

    energetically favorable. How

    does

    this arrangement result in higher

    entropy

    for the system, and thus make

    bilayer formation energetically favorable?

    A)

    Water molecules form

    cagelike structures around hydrophobic molecules.

    B)

    Fatty

    acid tails

    are

    highly saturated

    and flexible.

    C)

    Polar head

    groups

    form

    a

    hydrogen-bonding

    network

    at

    the interface

    with water.

    D)

    Hydrogen

    bonds fbrm between neighboring

    polar

    head

    groups

    in

    the

    bilayer.

    Which of the following

    statements is true?

    A) In

    eukaryotes,

    all

    membrane-enclosed

    organelles

    are

    surrounded

    by one lipid bilayer.,

    B) Membrane lipids frequently flip-flop

    between

    one

    monolayer

    and

    the

    other.

    C)

    Phospholipids

    will

    spontaneously form

    liposomes in nonpolar

    solvents.

    D) Membrane lipids

    difhrse within

    the

    plane

    of the membrane.

    A

    bacterium is suddenly expelled

    from

    a

    warm human

    intestine into

    the

    cold world

    outside. Which

    of the

    following

    adjustments might the

    bacterium make

    to

    maintain

    the

    same level

    of membrane

    fluidity?

    A)

    Decrease

    the amount of

    glycolipids

    in the

    membrane.

    B) Produce lipids

    with hydrocarbon

    tails that

    are

    longer

    and have fewer

    double bonds.

    C)

    Produce

    lipids with

    hydrocarbon tails

    that

    are

    shorter and have more double bonds.

    D) Decrease

    the amount

    of cholesterol

    in

    the

    membrane.

    Some

    lipases

    are

    able

    to

    cleave

    the

    covalent

    bonds between

    the

    glycerol

    backbone and

    the attached

    fatty

    acid.

    What final

    products

    do

    you

    expect

    to accumulate

    through the action

    of

    the enzyme

    monoacylglycerol

    lipase?

    A)

    free

    phosphate

    and

    glycerol

    B)

    glycerol

    and free fatty

    acid

    C) sterol and

    glycerol

    D)

    phosphoglycerol

    and free

    fatty acid

    Which of

    the

    following

    phospholipid

    precursors is the most

    hydrophobic?

    A)

    glycerol

    B)

    phosphate

    C)

    triacylglycerol

    D)

    diacylglycerol

    40.

    41.

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  • 8/17/2019 Cell Biology Test 3

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    extracellular

    space

    43.

    44.

    42.

    Three

    phospholipids

    X,

    Y,

    and Z

    are

    dishibuted in

    the

    plasma

    membrane

    as indicated

    in Figure

    Ql1-14.

    For which

    of

    these

    phospholipids

    does a

    flippase

    probably

    exist?

    Figure

    Qf

    l-14

    A) Y andZ

    B) XandY

    C) X

    only

    D) Zonly

    New

    membrane

    phospholipids

    are synthesizedby

    enzymes

    bound to the

    membrane.

    side

    of

    the

    A) luminal,

    Golgi

    B) extracellular,plasma

    C) cytosolic,mitochondrial

    D)

    cytosolic,endoplasmicreticulum

    Membrane

    synthesis in the cell

    requires

    the

    regulation

    of

    growth

    for

    both halves

    of the

    bilayer and

    the

    selective retention

    of certain types

    of lipids

    on one side

    or the other.

    Which

    group

    of en4anes

    accomplishes both

    of these tasks?

    A)

    convertases

    B)

    glycosylases

    C)

    phospholipases

    D) flippases

    Membrane

    curvature is influenced

    by the differential

    lipid

    composition

    of the

    two membrane

    monolayers.

    Which factor

    do

    you

    think

    has

    the largest impact

    on

    the curvature

    of

    biological

    membranes?

    A)

    charge of

    the

    lipid

    head

    group

    B)

    length

    of

    the

    hydrocarbon

    tails

    C) size of the lipid

    head

    group

    D)

    amount

    of

    cholesterol

    46.

    In

    the

    photosynthetic

    archaean

    Halobacterium halobium,

    a

    membrane transport

    protein called

    bacteriorhodopsin

    captures

    energy from

    sunlight

    and

    uses

    it to

    pump protons

    out

    of

    the

    cell.

    The resulting

    proton gradient

    seryes

    as

    an energy

    store that

    can

    later

    be

    tapped to

    generate

    ATP.

    Which

    statement

    best

    describes how bacteriorhodopsin

    operates?

    A)

    The

    absorption

    of

    sunlight

    triggers

    a

    shift in

    the conformation

    of

    the

    protein's

    seven,

    membrane

    spanning a

    helices,

    allowing

    a

    proton

    to

    leave the

    cell.

    B) The absorption

    of sunlight

    triggers the

    activation

    of

    an

    enzyme

    that

    generates

    ATP.

    C)

    The absorption

    of sunlight

    triggers

    a

    contraction

    of the

    b barrel that

    acts as

    the

    protein's

    central

    channel,

    squeezing a

    proton

    out

    ofthe

    cell.

    D)

    The

    absorption of

    sunlight triggers

    a

    restructuring

    of bacteriorhodopsin's

    otherwise

    unstructured

    core

    to

    form

    the channel through

    which

    a

    proton

    can exit the

    cell.

    45.

    cytosol

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    48.

    47.

    Plasma membranes

    are

    extremely

    thin

    and

    fragile,

    requiring

    an

    extensive

    support network

    of fibrous

    proteins.

    This network is called

    the

    A)

    attachment

    complex.

    B)

    spectrin

    C)

    cort€x.

    D)

    cytoskeleton

    The lateral movement

    of

    transmembrane

    proteins

    can

    be

    restricted

    by several different

    mechanisms.

    Which mechanism

    best describes the

    process

    by which focal adhesions

    are

    formed

    to

    promote

    cell

    motilitf

    A)

    proteins

    are

    tethered

    to

    the

    extracellular matrix

    B)

    proteins

    are

    tethered

    to the

    proteins

    on the

    surface

    of

    another

    cell

    C)

    protein

    movement

    is limited by

    the

    presence

    of a diffusion barrier

    D)

    proteins

    are tethered to the cell cortex

    The lateral movement of kansmembrane

    proteins

    can

    be

    restricted

    by several different

    mechanisms.

    Which mechanism

    best describes

    the

    process

    by which

    an

    antigen-presenting

    cell tiggers

    an

    adaptive

    immune

    response?

    A)

    proteins

    are

    tethered

    to

    the

    extracellular

    matrix

    B)

    proteins

    are

    tethered to

    the

    proteins

    on the surface

    of

    another

    cell

    C)

    protein

    movement

    is limited

    by the

    presence

    of a diffirsion barrier

    D)

    proteins

    are tethered

    to

    the

    cell cortex

    Diversity among

    the

    oligosaccharide

    chains found in the

    carbohydrate coating

    of the cell

    surface

    can

    be

    achieved

    in which of

    the

    following

    ways?

    A)

    varying

    the types of linkages

    between

    sugars

    B)

    varying the number

    of branches in

    the chain

    C)

    varying the types of

    sugar

    monomers used

    D) all

    of the above

    49.

    50.

    Page I I