cell-cell communication, sensing and chemotaxis
DESCRIPTION
Cell-Cell Communication, Sensing and Chemotaxis. N Kuldell for 20.020 Spring 2009. Heidelburg iGEM 2008: ecolicense to kill. Part 1: Sensing. Background information. Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi. Cuttlefish: master of camouflage. - PowerPoint PPT PresentationTRANSCRIPT
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Cell-Cell Communication, Sensing and Chemotaxis
N Kuldell for 20.020Spring 2009
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Heidelburg iGEM 2008: ecolicense to kill
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Part 1: Sensing
Background information
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Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi
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Cuttlefish: master of camouflage
During the day they lie buried in the bottom of the ocean; at night they swim and hunt for food.
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Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi
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Photo from B. Bassler, Fig from:
Genes & Dev. 2001. 15: 1468-1480 doi: 10.1101/gad.899601
AI-2AI-1Few cells: Autoinducers diffuse
away
More cells: Autoinducers initiate
signaling, transcription
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LuxS= enzyme that produces autoinducer 2 (AI2)…diffuses through the media
Prey Cell
Predator Cell
LuxP, LuxQ = receptor that binds (AI2)…induces chemotaxis….how?
Cell-Cell Communication iGEM context: “prey” pathogen, “predator” killer
Nature (2002) 415: 545
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Sensing and Signaling Cascades
natural context: “TCS” 2 component signalingAI-2AI-1
TCS
“SHK” sensor histidine kinase
“RR” response regulator
Stimulus
Activates SHK
Transfers to RR
Output
P
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Sensing and Signaling Cascades
natural context: “TCS” 2 component signaling
TCS
“SHK” sensor kinase
“RR” response regulator
Stimulus
Activates SHK
Transfers to RR
Output
P
Plant Physiol. (1998) 117: 723
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Sensing and Signaling Cascades
natural context: “TCS” 2 component signaling
TCS
“SHK” sensor kinase
“RR” response regulator
Stimulus
Activates SHK
Transfers to RR
Output
P
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
CCW, default, tumble
CW, signal, swim
TarChe flagella
J Bacteriol. (2000)182: 6865
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Sensing and Signaling Cascades
iGEM context: chimeric receptor