cell division and mitosis chapter 8. 11/19-11/21 complete photo graph complete photo graph review...
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Cell Division and Mitosis
Chapter 8
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11/19-11/21
Complete photo graph Review for exam Essay question topics Begin cell repro Ppoint Not an old persons disease case
study Cell Energy Exam on Friday
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Review Topics
Photo vs Cellular resp. (location, products)
Heat lost Define Respiration vs Cellular resp Equation What actually “carries” the energy? Stages in order What is oxidative Phosphorylation?
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Locations of stages Oxygen or no oxygen Net gains pH at the end What makes your muscles sore? What if your Mito’s couldn’t
complete the process?
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Essay 1 Making energy at different time
frames. Essay 2 Heterotroph, Autotroph, Aerobic,
Anaerobic, Eukaryote,Prokaryote
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HOMEWORK
12/2 Pre/post,Read pages 126-129
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Math moments
If you made $ 30,000 per hour, what would you make in a day?
If you were given $ 8.00 per year from the year you were born what would you have now? What would you have when you are 75 years old?
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Science Moments
If you lost 30,000 skin cells per hour, what would you lose in a day?
If you lost 8.00 lbs. of skin per year from the year you were born how many lbs. would you have lost by now? How much would you have lost when you are 75 years old?
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Like Begets Like….
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Buzz Words
Genes Chromosomes Asexual Sexual Generation Cell division Reproduction
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Why is it necessary for cells to divide?
DNA overload To improve material exchange
Surface area to volume
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Cell Size
Surface Area (length x width x 6)
Volume (length x width x height)
Ratio of Surface Area to Volume
Ratio of Surface Area to Volume in Cells
Section 10-1
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Understanding Cell Division
What instructions are necessary for inheritance?
How are those instructions duplicated for distribution into daughter cells?
By what mechanisms are instructions parceled out to daughter cells?
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Reproduction
Parents produce a new generation of cells or multicelled individuals like themselves
Parents must provide daughter cells with hereditary instructions, encoded in DNA, and enough metabolic machinery to start up their own operation
Like begets like..
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Division Mechanisms
Eukaryotic organisms
Mitosis
Meiosis
Prokaryotic organisms
Prokaryotic fission (Binary fission)
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Roles of Mitosis
Multicelled organisms
Growth
Cell replacement
Some protistans, fungi, plants,
animals
Asexual reproduction EXPLAIN!!!
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Chromosome A DNA molecule & attached proteins Duplicated in preparation for mitosis When the chromosomes are just a mass of fibers it is called
a Chromatin
one chromosome (unduplicated)
one chromosome (duplicated)
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Chromosome Number
Sum total of chromosomes in a cell Somatic cells
Chromosome number is diploid (2n) Two of each type of chromosome
Gametes Chromosome number is haploid (n) One of each chromosome type
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Human Chromosome Number
Diploid chromosome number (n) = 46
Two sets of 23 chromosomes each One set from father One set from mother
Mitosis produces cells with 46 chromosomes--two of each type
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Lots of DNA
Stretched out, the DNA from one human somatic cell would be more than two meters long
A single line of DNA from a salamander cell would extend for ten meters
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Organization of Chromosomes
DNA and proteinsarranged as cylindrical fiber
DNA
histone
one nucleosome
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Cell Cycle
Cycle starts when a new cell forms
During cycle, cell increases in mass and duplicates its chromosomes
Cycle ends when the new cell divides
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Fig. 8.4, p. 130
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Interphase
Usually longest part of the cycle
Cell increases in mass
Number of cytoplasmic components
doubles
DNA is duplicated
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Stages of Interphase
G1 Interval or gap after cell division
S Time of DNA synthesis (replication)
G2 Interval or gap after DNA replication
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Control of the Cycle
Once S begins, the cycle
automatically runs through G2 and
mitosis
The cycle has a built-in molecular
brake in G1
Cancer involves a loss of control
over the cycle, malfunction of the
“brakes”
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Stopping the Cycle
Some cells normally stop in
interphase
Neurons in human brain
Arrested cells do not divide
Adverse conditions can stop cycle
Nutrient-deprived amoebas get stuck in
interphase
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Cell Cycle Regulators
Cyclin (protein) regulate the timing of the cell cycle
Cancer cells do not respond to these “signals”
This results in masses called tumors
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A sample of cytoplasm is removed from a cell in mitosis.
The sample is injected into a second cell in G2
of interphase.
As a result, the second cell enters mitosis.
Effect of Cyclins
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Skin Cancer
Asymmetrical, Borders, Color, Diameter, Elevation
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Essay Question 1
Compare and contrast mitosis and meiosis. Pay extra attention to crossing over, number of daughter cells and chromosome number. Based on your comparison, explain how these two processes play an important role in reproduction and evolution.
List and describe the main events of the cell cycle.
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Mitosis
Period of nuclear division Usually followed by cytoplasmic
division Four stages:
Prophase
Metaphase
Anaphase
Telophase
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nucleusplasmamembrane
pair of centrioles
chromosomesnuclear envelope
CELL AT INTERPHASE EARLY PROPHASE LATE PROPHASE
TRANSITION TO METAPASE
Fig. 8.7a, p. 132
The cell duplicates its
DNA, prepares for
nuclear division
Mitosis begins. The DNA and its
associated proteins have started to
condense. The two chromosomes
color-coded purple were inherited
from the female parent. The other
two (blue) are their counterparts.,
inherited from the male parent.
Chromosomes continue to
condense. New
microtubules become
assembled. They move one
of the two pairs of centrioles
to the opposite end of the
cell. The nuclear envelope
starts to break up.
Now microtubules penentrate
the nuclear region. Collectively,
they form a bipolar spindle
apparatus. Many of the spindle
microtubules become attatched
to the two sister chromatids of
each chromosome.
MITOSIS
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METAPHASE ANAPHASE TELOPHASE INTERPHASE
Fig. 8.7b, p. 133
All chromosomes have become
lined up at the spindle equator.
At this stage of mitosis (and of
the cell cycle), they are most
tightly condensed
Attachments between the
two sister chromatids of each
chromosome break. The two
are separate chromosomes,
which microtubules move to
opposite spindle pores.
There are two clusters
of chromosomes, which
decondense. Patches of
new membrane fuse to form
a new nuclear envelope.
Mitosis is completed.
Now there are two
daughter cells. Each
is diploid; its nucleus
has two of each type
of chromosome, just
like the parent cell.
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Longest Phase
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The Spindle Apparatus
Consists of two distinct sets of
microtubules
Each set extends from one of the cell
poles
Two sets overlap at spindle equator
Moves chromosomes during mitosis
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Spindle Apparatus
one spindle pole
one of the condensed chromosomes
spindle equator
microtubules organized as a spindle apparatus
one spindle pole
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Stages of Mitosis
Prophase
Metaphase
Anaphase
Telophase
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Early Prophase - Mitosis Begins
Duplicated chromosomes begin to condense
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Late Prophase
New microtubules are assembled
One centriole pair is moved toward opposite pole of spindle
Nuclear envelope starts to break up
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Transition to Metaphase
Spindle formsSpindle microtubules become attached to the two sister chromatids of each chromosome
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Metaphase
All chromosomes are lined up at the spindle equator
Chromosomes are maximally condensed
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Anaphase
Sister chromatids of each chromosome are pulled apart
Once separated, each chromatid is a chromosome
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Telophase
Chromosomes decondense
Two nuclear membranes form, one around each set of unduplicated chromosomes
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Results of Mitosis
Two daughter nuclei
Each with same chromosome number as parent cell
Chromosomes in unduplicated form
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Metaphase
Prophase
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Prophase
Interphase
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Anaphase
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Telophase
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Cytoplasmic Division
Usually occurs between late
anaphase and end of telophase
Two mechanisms
Cell plate formation (plants)
Cleavage (animals)
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Cytokinesis
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Cell Plate Formation
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Animal Cell Division
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Essay Question #2
Describe how cancer cells are different from other cells and how they form. Based on these differences, explain why cancer is so difficult to cure and explain how tumors form.
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Essay question 3
You have been introduced to a variety of abnormalities found in the human based on the number and location of chromosomes. Choose one of these” syndromes” and illustrate its karyotype. In addition, explain in detail the genetic condition as well as the biological features affected by it.
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There is a single bacterium in a bottle at 11:00 pm, and it is a type that doubles once every minute. The bottle will be completely full of bacteria at 12:00 midnight - exactly one hour.
In your opinion, what percentage of the bottle will be full when the bottle starts to look full? For what amount of time between 11:00 and 12:00 would they have plenty of room to grow and spread out? If you were a lab researcher, in the lab, at what time between 1:00 and midnight might you look at the bottle and think, "I'd better get a bigger container for those bacteria!"
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Culturing Cells
Growing cells in culture allows researchers to investigate processes and test treatments without danger to patients
Most cells cannot be grown in culture
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HeLa Cells
Line of human cancer cells that can be grown in culture
Descendents of tumor cells from a woman named Henrietta Lacks
Lacks died at 31, but her cells continue to live and divide in labs around the world
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Regulating the Cell Cycle
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Pre-Natal Tests - help to determine if an at risk baby is healthy
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Sex chromosomes
Human Karyotype
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Sex Chromosomes
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Female with Turner Syndrome (XO)
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Klinefelder Syndrome
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Klinefelter’s Syndrome
47, XXY
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Klinefelter Syndrome (XXY)
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Down Syndrome (trisomy 21),
47,XY,+21.
47: the total number of chromosomes (46 is normal).
XY: the sex chromosomes (male).
+21: designates the extra chromosome as a 21.
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Down Syndrome
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Gene Therapy - an absent or faulty gene is replaced by a working gene
Has not been very successful
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Normal hemoglobin gene
Bone marrow cell
Chromosomes
Genetically engineered virus
Nucleus
Bone marrow
Figure 14-21 Gene Therapy
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Cloning
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A donor cell is taken from a sheep’s udder. Donor
Nucleus
These two cells are fused using an electric shock.
Fused Cell
The fused cell begins dividing normally.
EmbryoThe embryo is placed in the uterus of a foster mother.Foster
Mother
The embryo develops normally into a lamb—Dolly
Cloned Lamb
Egg Cell
An egg cell is taken from an adult female sheep.
The nucleus of the egg cell is removed.
Figure 13-13 Cloning of the First Mammal
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Stem Cells
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1. What phase ?
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2. What phase ?
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3. What phase ?
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4. What phase ?
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5. What is the longest phase of the cell cycle ?
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6. What is the longest phase of
mitosis?
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7. List (in order) the phases of
mitosis
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8. List two outcomes of Interphase
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9. How many chromosomes do you have in you
sex cells ?
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10. Name the phase
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BONUS
Name and explain two reasons why cells must divide