cells and macromolecules section a proteindna. a1 cellular classification a2 subcellular organelles...
TRANSCRIPT
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Cells and macromolecules
Section A
Protein DNA
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A1 Cellular classification
A2 Subcellular organelles
A3 Macromolecules
A4 Large macromolecular Assemblies
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A1-1 Eubacteria
A1-2 Archaea
A1-3 Eukaryotes
A1-4 Cellular differentiation
A1 Cellular classification
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Prokaryotes原核细胞
Eukaryotes真核细胞
Eubacteria 真细菌
Archaea 古细菌
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The Eubacteria are one of two subdivisions of the prokaryotes.
A1-1 Eubacteria
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• Cell wall: to prevent cell lysis in environments of low osmolarity
• Plasma membrane: lipid bilayer and embedded proteins for small molecule exchange
• Genetic materials: nucleiod (single and circular chromosome), plasmid
• Ribosmes: protein synthesis machinery
• Pili: to allow the cell to attach to other cells and surfaces
• Flagella: whose rotating motion allows the cell to swim
A1-1 Eubacteria
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Escherichia. coli E.coli has a genome size of 4600 kb, which is sufficient genetic information for about 3000 proteins.
Mycoplasma genitalium It has only 580 kb of DNA and encodes just 470 proteins.
A1-1 Eubacteria
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A1-2 Archaea
• The second subdivision of the prokaryotes
• Structurally, they are similar to eubacteria
• The display some unusual biochemical
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Eukaryotes are defined by their possession of
membrane-enclosed organelles with specialized
metabolic function.
Eukaryotic cells tend to be larger than prokaryotes.
Eukaryotes are classified taxonomically into four
kingdoms: animals, plants, fungi and protists.
A1-3 Eukaryotes
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Organelles
Cytoskeleton
Controls the shape and movement
of the cellOrganizes some metabolic
functions
Genetic system
A1-3 Eukaryotes
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Prokaryotes EukaryotesEubacteria Archaea
Structural features
1-10 mm, no distinct subcellular organelles, pili, flagella
10-100 mm,Organelles
Biochemistry
rRNA molecules are different among these kingdoms Ester linkage Ether linkage Ester linkageEnergy production, metabolism
Replication, transcription and translation
Differentiation Formation of spores Embryonic cell differentiation
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Phylogenetic tree determined by rRNA sequence comparisons(rRNA 序列比较得到的系统发育树)
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A1-4 Cellular differentiation
The main molecular reason: change of the genes being transcribed, but not that of the DNA content.
Regulated by developmental control genes, mutations in these genes result in abnormal body plans.
Definition: The daughter cells change their patterns of gene
expression to become functionally different from the parent cell
after cell division.
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A1-4 Cellular differentiation
•Spore formation
among prokaryotes
and lower
eukaryotes
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tadpole
Embryonic cell differentiate into highly specialized cells
among higher eukaryotes.
A1-4 Cellular differentiation
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A2-1 Nuclei
A2-2 Mitochondria and chloroplasts
A2-3 Endoplasmic reticulum
A2-4 Microbodies
A2-5 Organelle isolation
A2 Subcellular organelles
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Nuclei: The membrance-bound nucleus contains the bulk of the
cellular DNA in multiple chromosomes.
A2-1 Nuclei
DNA replication
RNA transcription & processing
Ribosome assembly
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Oxidation of nutrients to genetate energy in the form of adenosine 5’-triphosphate(ATP).
Main function: cellular respiration/ATP production via oxidative phosphorylation
A2-2 Mitochondria
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A2-2 Chloroplasts
The chloroplasts of plants are the site of photosynthesis, the light-dependent assimilation of CO2 and water to form carbohydrates and oxygen.
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A2-3 Endoplasmic reticulum
The endoplasmic reticulum is an extensive membrane system within the cytoplasm and is continuous with the nuclear envelope.
Two forms are visible in most cells. Smooth endoplasmic reticulum Rough endoplasmic reticulum
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The rough endoplasmic reticulum is so-called because of the presence of many ribosomes. These ribosomes specifically synthesize proteins intended for secretion by the cell or those destined for the plasma membrane or certain organelles.
A2-3 Endoplasmic reticulum
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The Smooth endoplasmic reticulum carries many membrane-bound enzymes,
including those involved in the biosynthesis of certain lipids and the oxidation
and detoxificatin of foreign compounds such as drugs.
A2-3 Endoplasmic reticulum
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Lysosomes: Contain a variety of digestive enzymes capable of degrading proteins, nucleic acids, lipids and carbohydrates.
Peroxisomes: Confine highly reactive free radicals and hydrogen peroxide.
Glyoxysomes: Carry out the reactions of the glyoxylate cycle.
A2-4 Microbodies
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A2-5 Organelle isolation
Differential centrifugation
Rate zonal centrifugation
Equilibrium centrifugation
To call the parameter which characterizes the movement of the particle at the centrifugal force place.
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A3 Macromolecules
A3-1 Macromolecules
A3-2 Complex macromolecules
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A3-1 Macromolecules
Proteins
Proteins are polymers of amino acids linked together by
peptide bonds.
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A3-1 Macromolecules
Nucleic acids Nucleic acids are polymers of nucleotides.
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Polysaccharides are polymers of simple sugars covalently linked by glycosidic bonds.
A3-1 Macromolecules
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Lipidsindividual lipids are not strictly
macromolecules, large lipid
molecules are built up from
small monomeric units and
involved in many
macromolecule assembly
A3-1 Macromolecules
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Many macromolecules contain covalent or noncovalent
associations of more than one of the major classes of large
biomolecules, which can greatly increase the functionality
or structural capabilities of the complex.
A3-2 Complex macromolecules
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NucleoproteinsAssociations of nucleic acid and protein telomerase / ribonuclease P
GlycoproteinsContain both protein and carbohydraty componentsThey are important components of cell membranes and mediate cell-cell recognition.
Proteoglycans / MucoproteinsLarge complexes of protein and mucopolysaccharideFound in bacterial cell walls and in the extracellular space in connective tissue
A3-2 Complex macromolecules
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Lipid-linked proteinsHave a covalently attached lipid componemt. This groups serve to anchor the proteins in membranes through hydrophobic interactions with the membrance lipids and also promote protein-protein association.
Lipoproteins The lipids and proteins are linked noncovalentlyLipid transport
GlycolipidsCovalently linked lipid and carbohydrate include cerebrosides and gangliosides. Abundant in the membrans of brain and nerve cells
A3-2 Complex macromolecules
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A4-1 Protein complexes
A4-2 Nucleoprotein
A4-3 Membranes
A4-4 Noncovalent interactions
A4 Large macromolecular Assemblies
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A4-1 Protein complexes
The eukaryotic
cytoskeleton consists of
various protein
complexes:
Microtubules
Microfilaments
Intermediate filaments
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A4-2 Nulceoprotein
Nucleoproteins comprise both nucleic acid and protein.
Ribosomes are the large cytoplasmic ribonucleoprotein.
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Chromatin: deoxyribonucleoprotein consisting
of DNA and histones to form a repeating unit called nucleosome
Viruses:protein capsid + RNA or DNA
Telomerase: replicating the ends of eukaryotic chromosomes. RNA acts as the replication template, and protein catalyzes the reaction
A4-2 Nulceoprotein
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A4-3 Membranes
peripheral transmembrane protein integral transmembrane protein transmembrane protein
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Charge –chargeCharge –charge interactions operate between ionizable groups of opposite charge at physiological pH.
Charge –dipole/ dipole -dipoleCharge –dipole form when either or both of the participants is a dipole due to the asymmetric distribution of charge in the molecule.
Dispersion force/ hydrophobic interactionTransient dipoles arising from the motion of their electrons.
A4-4 Noncovalent interactions
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(偶极 )
(uncharged molecules)
A4-4 Noncovalent interactions
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Van der waals forcesNoncovalent associations between electrically neutral molecules.
Hydrogen bondsForm between a covalently bonded hydrogen atom on a donor group and a pair of nonbonding electrons on an acceptor group.
A4-4 Noncovalent interactions