CentritechSeparation System

Technology

CentritechCentritech®®

• Single-Use Centrifugation Platform

• Sterile disposable inserts

• No CIP/SIP

• Scalable Solutions (up to 120 L/h)

• Fully automated, hands-off production

Centritech ApplicationsCentritech Applications

Perfusion

• Hybridoma cells for MAb production

• CHO cells on micro-carriers for protein production

• Insect cells for protein production

Cell Harvesting

• HEK cells for cell banking

• Myeloma cells for cell therapy research

• Inoculum preparation

• Viral Vector Production

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Centritech

Hollow Fiber

*Rotor speed

reduced on

Day 17

Healthier BioreactorsHealthier Bioreactors

Varying the G-force* enables the removal of Dead Cells from the Culture

Higher YieldsHigher Yields

Productivity Comparison of Two Perfusion

Technologies

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Centritech

Hollow Fiber

C E N T R I T E C H I N S E R T

• Disposable

• USP Class VI Material

• Pre-Sterilized, No SIP

• Color-Coded Tubing

• No Rotating Seal

Separation CycleSeparation Cycle

(Overhead View of System)

Cells are separated under low g-forces while supernatant is continuously collected

Separation CycleSeparation Cycle

(Overhead View of System)

Concentrated Cells are Isolated by inflating a sealed air barrier which lays behind the separation insert cutting off

the feed from the concentrated cells

Isolation CycleIsolation Cycle

(Overhead View of System)

Concentrated cells (dark green) are

Discharged out via the bottom pump

Discharge CycleDischarge Cycle

Centritech Lab IIICentritech Lab III

• Adjustable Flow Rate

– up to 10,7 L/h

• Adjustable Capacity

– up to 300L bioreactor

• Variable G-force

– up to 253 x g

Centritech CELL IICentritech CELL II

• Adjustable Flow Rate

– up to 120 L/h

• Adjustable Capacity

– up to 3.000L bioreactor

• Variable G-force

– up to 317 x g

Centritech ScalabilityCentritech Scalability

99+% Viable Cell Recycle

2.5 x 104

cells/ml

20 L Bioreactor

Centritech Lab II

5.0 x 106

Cells/ml

2000 L Bioreactor

Centritech Cell

99+% Viable Cell Recycle

Cell Free Supernatant to Downstream Processing

2 L2 L

Inoculum PreparationBioreactor Loop

Production BioreactorLoop

CARR Centritech CARR Centritech -- SummarySummary

• Single-Use Centrifugation Platform

– Easy to operate

– Pre-sterilized disposable inserts

– Eliminates CIP/SIP

– Perfusion and Harvest applications

• Scalable Solutions (up to 120 L/h)

• Promotes Healthier Bioreactors

– Removes dead cells

• Closed, automated system

• Used in FDA cleared cGMP facilities

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