LT-2/ LT-3/ LT-4 No. of Printed Pages : 12

CERTIFICATE PROGRAMME IN LABORATORY TECHNIQUES (CPLT)

Term-End Examination December, 2012

01257

LT- 2 : Laboratory Techniques in Biology LT- 3 : Laboratory Techniques in Chemistry LT- 4 : Laboratory Techniques in Physics

Time Allowed : 3 Hours Maximum Marks : 150

Note : (i) This question paper contains three parts, one each for LT-2, LT-3 and LT-4 courses.

Maximum time allowed for each part is 1 hour. (ii) Students are required to answer all the three parts in three separate answer books. Write your

Enrolment number, Course code and Course title clearly on each of the three answer books.

(iii) Marks are indicated against each question.

LT-2 : Laboratory Techniques in Biology Time : 1 hour Maximum Marks : 50

All questions are compulsory.

1. Attempt all parts of this question. 4 (a) Fill in the blanks :

(i) Instrument used to measure the intensity of color of a coloured solution is

called

(ii) The use of two stains together is called

(iii) Arthropods shed their exoskeleton periodically by process of (iv) Group of organisms that can interbreed to produce viable offspring is called

(b) State two distinguishing features between each of the following pairs : 4 (i) Acid and Basic dyes

(ii) Aquarium and Vivarium

LT-2/ LT-3/ LT-4 1 P.T. O.

(c) Mark the following statements as True or False : 4

(i) Earthworms and leeches are hermaphrodite.

(ii) Light of short wavelength is of lower energy than light of long wavelength

(iii) Dehydration of the mount is done by using increasing strength of alcohol

series.

(iv) Resolution is the ability of an optical system to be focused by adjustment of

the lens tube.

(d) Name two chemicals used as preservatives. 1

(e) Name the instrument used for : 2

(i) Section Cutting

(ii) Separation of suspensions

2. Attempt any five parts of this question.

(a) Describe techniques to collect plant and animal specimens. 5

(b) Compare and contrast the operating principle of the compound microscope with

that of transmission electron microscope.

5

(c) Write down the nutritional factors affecting growth of micro organisms. 5

(d) Discuss general features of biology laboratories. 5

(e) What is mounting ? Describe two methods of mounting insects. 5

(f) Explain the steps involved in making a permanent slide. 5

3. Explain the maintenance, preservation and disposal of microbial cultures. 10

OR

With the help of a suitable diagram, describe the working of a distillation unit. 10

LT-2/LT-3/LT-4 2

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LT-2/LT-3/LT-4 4

LT-3 : Laboratory Techniques in Chemistry

Time : 1 hour Maximum Marks : 50

Note : Answer all the questions.

1. Fill in the blanks with appropriate words chosen from those given in the brackets alongside. Answer any ten. 1x10=10

(a) Vinegar is ( solution, suspension)

(b) Ether will form the layer when shaken with water. (upper, lower)

(c) Tap water can be tested using . (silver nitrate, sodium nitrate)

(d) EDTA is used in . (complexometry, argentometry)

(e) Rf value can be than one. (more, less)

(f) Leucine is an . (amino acid, amino alcohol)

(g) Starch forms a in water. (suspension, complex)

(h) Chloroform is a . (hydrocarbon, chloro compound)

(i) Methanol is a solvent. (polar, non polar)

(j) A Bunsen flame is the hottest just the blue cone. (above, below)

(k) On heating the solubility of most solids . (increases, decreases)

(1) Borosilicate glass has a softening temperature than soda glass. (lower, higher)

(m) Lime water is a saturated solution of . (calcium carbonate, calcium hydroxide)

2. Draw a labelled diagram of any one of the following : 10 (a) A set up for paper Chromatography

(b) Semi-micro technique for boiling point determination of small samples.

3. Write short notes on any two of the following : 2x5=10

(a) Filtration (b) Distillation

(c) Columnchromatography

LT-2/LT-3/LT-4 5 P.T.O.

4. (a) Answer any three parts : 2x3=6

(i) What is standard solution ?

(ii) What are azeotropic mixtures ? Give one suitable example.

(iii) What are stationary and mobile phases in Chromatography ?

(iv) What causes hardness of water ?

(b) Enlist four Characteristics of a solute for preparing a standard solution. 4

5. (a) Explain why ? (Attempt any three)

(i) Glass stoppers should be avoided on aqueous sodium hydroxide bottle.

(ii) Silver nitrate should be kept in dark colored bottles.

(iii) Metal sodium should be kept in paraffin.

(iv) What is difference between deionized and distilled water ?

(v) For working on borosilicate glass, Oxygen -LPG mixture is used.

(b) Give the method of preparation of :

(i) iodine solution

(ii) starch solution

3x2=6

2x2=4

LT-2/LT-3/LT-4 6

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LT-2/ LT-3/ LT-4

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LT-2/LT-3/LT-4 8

LT-4 : Laboratory Techniques in. Physics

Time : 1 hour Maximum Marks : 50

Note : Attempt all questions.

1. Attempt any five : 3x5=15

(a) What do you understand by positive and negative zero error in a Vernier

Calliper ?

(b) How can you avoid back lash error in a screw gauge experiment ?

(c) Spring balance measures weight and beam balance measures mass. Justify the

statement.

(d) Write the symbol of following component :

(i) Transformer

(ii) Inductor

(iii) Variable Power Supply

Convert 90°F into Celsius.

What is an electromagnet ? How do you decide the polarity of an electromagnet ?

Draw the ray diagram showing the image formation in a compound microscope.

2. Attempt any five parts : 7x5=35

(a) (i) What general utilities do you expect in a Physics Laboratory ? 3

(ii) In a Vernier Calliper 50 divisions of VS (Vernier Scale) coincide into 49 4

divisions of main scale. 1 MSD = 0.5mm. Calculate the least count of the

vernier calliper.

(b) (i) Name six mechanical tools used in the Physics Laboratory. 3

(ii) If a candle is placed at a distance of 15 cm from a concave mirror of focal 4

length 10 cm, calculate the distance at which image is formed ?

(c) (i) Mention main three components of a spectrometer. 3

(ii) Write the basic principle of a simple voltaic cell. 4

LT-2/LT-3/LT-4 9 P.T.O.

(d) (i) Identify the meter to be used for each of the following tasks and draw their symbols :

3

(ii)

(A) detecting 0.1 mA current

(B) Measuring 10 V potential difference

(C) Measuring 1 A current

Draw the IV characteristics of a resister and a Pn junction diode. 4

(e) (i) How are stationary waves generated on a sonometer wire ? 3

(ii) State the laws of reflection and laws of refraction. Depict the ray diagrams.

4 (f) (i) Plot the direct current and alternating current on a function of time.

4 (ii) Write three important properties of permanent magnets.

3 (g) (i) Why should you have metal covers for the jaws of a vice ?

3 (ii)

Explain with the help of a diagram, the measurement of an unknown resistance by a metre bridge. 4

LT-2/ LT-3/LT-4 10

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LT-2/ LT-3/ LT-4

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LT-2/LT-3/LT-4 12