chapter 6 ag-ab interactions
DESCRIPTION
Chapter 6 Ag-Ab Interactions. Nature of the Ag-Ab interaction Immunological assays. 4 types of noncovalent forces. Affinity The strength of the total noncovalent interactions between a single Ag-binding site on an Ab and a single epitope is the affinity of the Ab for that epitope. - PowerPoint PPT PresentationTRANSCRIPT
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Chapter 6 Ag-Ab Interactions
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Nature of the Ag-Ab interaction
Immunological assays
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4 types of noncovalent forces
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AffinityThe strength of the total noncovalent interactions between a single Ag-binding site on an Ab and a single epitope is the affinity of the Ab for that epitope.
AvidityThe strength of multiple interactions between amultivalent Ab and Ag is called the avidity.
Example: secreted pentameric IgM often has a lower affinity than IgG, but the high avidity of IgM, resulting from its higher valence, enables it to bind Ag effectively.
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Immunological Assays
1. Precipitation
2. Agglutination
3. Radioimmunoassay
4. Enzyme-linked Immunosorbent Assay
5. Western Blotting
6. Immunofluorescence
7. Flow Cytometry and Fluorescence
8. Immunoelectron Microscopy
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Precipitation Reactions:
Precipitation in fluidsPrecipitation in gels - radial immunodiffusion (Mancini method) - double immunodiffusion (Ouchterlony method)Immunoelectrophoresis
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Precipitation Reactions
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Lattice Formation(格子 )
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Double Immunodiffusion (Ouchterlony method)
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Immunoelectrophoresis
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Agglutination Reactions:
HemagglutinationBacterial AgglutinationPassive AgglutinationAgglutination Inhibition
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Hemagglutination
Agglutination No Agglutination No Ab
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Agglutination Inhibition
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Solid-phase Radioimmunoassay (RIA)
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Enzyme-linked Immunosorbent Assay (ELISA):
- Indirect ELISA
- Sandwich ELISA
- Competitive ELISA
- Chemiluminescence
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Elispot Assay
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Western Blotting
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Immunofluorescence
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Flow Cytometry
FACS:
Fluoresence-activatedCell sorter
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Immunoelectron microscopy
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