chapter 9: identification of saliva and misc. biological fluids

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Chapter 9: Identification of Saliva and Misc. Biological Fluids

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 Starch  Amylose  Amylopectin  Two types of amylases:  β-amylase ▪ Plant and bacterial sources  α-amylase ▪ Human ▪ Human salivary (HSA) ▪ Human pancreatic (HPA) Forensic Biology by Richard Li3

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Page 1: Chapter 9: Identification of Saliva and Misc. Biological Fluids

Chapter 9: Identification of Saliva and Misc. Biological Fluids

Page 2: Chapter 9: Identification of Saliva and Misc. Biological Fluids

Human salivary glands produce 1.0-1.5 liters per day

Mostly water Amylase- digests carbohydrates in the

oral cavity Identification of amylase indicates saliva Enzymes that cleave polysaccarides that

are composed of D-glucose units connected by (α1-4) linkages▪ Starch

Forensic Biology by Richard Li 2

Page 3: Chapter 9: Identification of Saliva and Misc. Biological Fluids

Starch Amylose Amylopectin Two types of amylases:

β-amylase▪ Plant and bacterial sources

α-amylase▪ Human▪ Human salivary (HSA)▪ Human pancreatic (HPA)

Forensic Biology by Richard Li 3

Page 4: Chapter 9: Identification of Saliva and Misc. Biological Fluids

Visual Examination ALS (470nm), Microscopic

Starch-iodine assays Iodine used to test for starch Not specific

Colorimetric assays Dye-labeled amylase substrates are

employed Phadebas test

Forensic Biology by Richard Li 4

Page 5: Chapter 9: Identification of Saliva and Misc. Biological Fluids

Immunochromatographic Assays RSID-Saliva

ELISA Monoclonal HSA antibody

RNA-based assays Certain genes are specifically expressed

in certain types of cells

Forensic Biology by Richard Li 5

Page 6: Chapter 9: Identification of Saliva and Misc. Biological Fluids

Vaginal secretions, Menstrual blood, Urine, Feces, Vomitus Less frequently encountered Can be probative in certain cases

Forensic Biology by Richard Li 6