chapter 9: identification of saliva and misc. biological fluids
DESCRIPTION
Starch Amylose Amylopectin Two types of amylases: β-amylase ▪ Plant and bacterial sources α-amylase ▪ Human ▪ Human salivary (HSA) ▪ Human pancreatic (HPA) Forensic Biology by Richard Li3TRANSCRIPT
Chapter 9: Identification of Saliva and Misc. Biological Fluids
Human salivary glands produce 1.0-1.5 liters per day
Mostly water Amylase- digests carbohydrates in the
oral cavity Identification of amylase indicates saliva Enzymes that cleave polysaccarides that
are composed of D-glucose units connected by (α1-4) linkages▪ Starch
Forensic Biology by Richard Li 2
Starch Amylose Amylopectin Two types of amylases:
β-amylase▪ Plant and bacterial sources
α-amylase▪ Human▪ Human salivary (HSA)▪ Human pancreatic (HPA)
Forensic Biology by Richard Li 3
Visual Examination ALS (470nm), Microscopic
Starch-iodine assays Iodine used to test for starch Not specific
Colorimetric assays Dye-labeled amylase substrates are
employed Phadebas test
Forensic Biology by Richard Li 4
Immunochromatographic Assays RSID-Saliva
ELISA Monoclonal HSA antibody
RNA-based assays Certain genes are specifically expressed
in certain types of cells
Forensic Biology by Richard Li 5
Vaginal secretions, Menstrual blood, Urine, Feces, Vomitus Less frequently encountered Can be probative in certain cases
Forensic Biology by Richard Li 6