chromatography catalog 2013/14 - moss instruments system.pdf · other hplc columns with inorganic...

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Chromatography Catalog

2013/14

Moss Instruments Co., Ltd.

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Contents

HPLC Columns ……………………………………………………………………………..3

Chromatographic Column Selection…………………………………………………………..3

How to Guarantee Good Performance and Lifetime……………………………………..….4

HPLC Stationary Phases and Their Applications Range………………………………..….5

Troubleshooting……………………………………………………………………………..….7

Guard Columns Selections……………………………………………………………………9

USP Column Listing and DEAIC Offerings………………………………………………….9

Mos Series HPLC Columns ………………………………………………………………….10

Routine Analysis Column…………………………………………………………………….10

Protein Analytical Columns…………………………………………………………………..20

Dynamic Axial Compression Column………………………………………………………22

Semi-prep/Preparative HPLC Columns……………………………………………………23

JiajieTM Guard Columns……………………………………………………………………..26

Dextrangel Column Special …………………………………………………………………27

Columns for Amino Acids Analysis System………………………………………………..28

HPLC Instruments……………………………………………………………………….…29

Assembly Unit……………………………………………………………………………… 29

P1201 HPLC Pump ………………………………………………………………………….29

P230 HPLC Pump……………………………………………………………………………30

UV1201 UV/VIS Detector…………………………………………………………………….30

UV230+/UV230II UV/Vis Detector……………………………………………………..……31

RI230II Refractive Index Detector…………………………………………………………..32

DAD230+ Diode Array Detector ……………………………………………………………32

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LU230/LU230II Low-pressure Gradient Unit………………………………………………34

DG230 On-line Degasser…………………………………………………………………..34

ZWII Column Oven………………………………………………………………………….35

AS1201 Auto-Sampler……………………………………………………………………..36

RO230/RO1201 Solvent Manager……………………………………………………….37

P230p Semi-Preparative HPLC Pump…………………………………………………..37

P270 Preparative HPLC Pump………………………………………………………….38

P1000+/P500+ Preparative HPLC Pump……………………………………………….38

P2000+ Preparative HPLC Pump……………………………………………………….39

HPLC System Configurations…………………………………………………………..40

Moss iChrom5100 HPLC System…………………………………………………………40

iChrom5100 Isocratic Systems Configurations…………………………………………40

iChrom5100 Binary High-pressure Gradient System Configurations…………………40

iChrom5100 Quaternary Low-pressure Gradient System Configurations…………...41

P1201 Isocratic Systems Configurations………………………………………………..41

P1201 High-pressure Gradient System Configurations……………………………....42

P230/P230II Isocratic Systems Configurations……………………………………….42

P230/P230II High-pressure Gradient System Configurations………………………43

Semi-prep Isocratic System Configurations …………………………………………..43

Prep. Isocratic System Configurations…………………………………………………44

Semi-prep Gradient System Configurations……………………………………………45

Prep. Gradient System Configurations…………………………………………………46

LU230/LU230II Quaternary Low-pressure Gradient System………………………….47

Amino Acids Analysis System…………………………………………………………48

Mos-AAK Amino Acids Analysis System……………………………………………….48

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Mos-AAP Amino Acids Analysis System……………………………………………….50

Chromatography Data-processing Workstation…………………………………..52

EC2006 Chromatography Data-processing Workstation ……………………………52

EC2000 Chromatography Data-processing Workstation……………………………53

EC2000 GPC Chromatography Data-processing Workstation…………………….53

EC2000DAD Chromatography Data-processing Workstation……………………..54

Dealers ……………………………………………………………………………...56

HPLC Accessories……….………………………………………………………..…57

Tubing and Connections………………………………………………………………57

Filters……………………………………………………………………………………59

Syringes…………………………………………………………………………………61

Columns Accessories…………………………………………………………………62

Instruments Accessories……………………………………………………………...63

4

HPLC Columns

Chromatographic Column Selection

There is a wide range of packing materials in HPLC, and the selection of them has great influence on the

separation performance, so it is very necessary to know of them for appropriate selection.

Silica and silica-based packings

A. Normal phase chromatography

The stationary phases for normal phase chromatography are usually silica and some polar bonded-phase

functional groups, such as Amino Propyl (NH2, APS), Propyl Nitrile (CN, CPS).

As the Si-OH in the surface of silica and other polar functional groups are highly polar, the sequence of

being separated is in line with their polarity in respective analytes, that is to say, the weaker its polarity,

the quicker being separated.

The mobile phases for normal phase chromatography is much weaker than stationary phases, such as

Hexane, Chloroform, and Methylene Chloride.

B. Reversed phase chromatography

The stationary phases for reversed phase chromatography are silica-based bonded phases with

non-polar functional groups on the surface of the silica.

The mobile phases for reversed phase chromatography are polar mixtures of water or buffered solvents

with methanol or acetonitrile.

The most polar analyte will be first dashed out among the sample, while the less polar ingredient will be

strongly retained.

Typical packings for reversed phase chromatography are C18 (ODS), C8 (MOS), C4 (Buty1), C6H5

(Phenyl).

Polymeric packings

Polymeric packings are mainly polystyrene-divinylbenzene or polymethacrylate，and etc，which can be

used from pH 1-14 as their leading advantage.

Comparing with silica-based C18 packing, these packings are more hydrolphobic; polymeric packings

with large pore are quite efficient to the separation of samples like protein.

The main disadvantage of current polymeric packings is the low column efficiency compared with sili-

ca-based packings.

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Other inorganic packings

Other HPLC columns with inorganic packings have been commercialized, but they are limited for special

use because of unique property, for example, graphitic carbon is gradually becoming a member of re-

versed phase packings. Separation with this kind of packings is different from silica-based alkyl-bonded

phases, for the graphite surface is the retention base, you don’t need any other surface modifications.

Normally, this column packing has stronger retention than alkyl-bonded silica or porous polymeric pack-

ings. Graphitic carbon can separate some geometrical isomer, and it can’t be dissolved by the HPLC

mobile phase, so it can be used in any pH range and temperature. Alumina can also be used in HPLC,

first, it has strong rigidity and could be made into stable chromatographic column bed; second, it can be

used with the mobile phase at pH 12. But alumina hasn’t been widely applied for its application area is

limited by the strong interactions with basic compounds. The new-type zirconia-based packings can also

be used in HPLC, but the only commercializd packing is polymer-coated porous microspherical zirconia,

which exihits at the environment of pH 1-14 and the highest temperature 100℃. As zirconia-based

packings have been studied for recent years, facing experiment difficulties, so their important purposes

and advantages are in process.

How to Guarantee Good Performance and Lifetime?

◇Read columns user manual carefully

◇Use well packed columns

◇Reduce pressure pulsation to avoid mechanical and thermal impact

◇Make use of guard column and on-line filter

◇Wash columns with strong solvents often

◇Completely filter samples and mobile phases, avoid impurity atoms and strong reserved com-

ponents

◇Use stable stationary phases (C18 is the most stable stationary phase)

◇Use organic buffer solvents on the operation of pH ranging 6-8

◇The best use temperature for columns is lower than 40℃

◇Silica-based columns should keep their mobile phase pH ranging 2.0-8.0

◇Add 200ppm NaN3 in water mobile phases and buffer solvents

◇If there are buffer solvents in the mobile phases, pay attention to use 95:5 water and organic

solvent to filter, and the organic solvent can’t less than 5%.

◇For storage or stay for a night, rinse out salt and buffer solvent and reserve with pure organic

mobile phase. (Ethylic-nitrile is best)

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HPLC Stationary Phases and Their Applications Range

Common

Name Alternative

Functional

Group

Normal

Phase

Reverse

Phase

Ion

Exchange Application

Silica SiO2 -OH √

Non-polar and moder-

ately polar organic

compounds.

SAS C1 -(CH3)3 √

Least retentive of all

alkyl group bonded

phases for non-polar

solutes.

Typically used for

moderately polar and

multi-functional com-

pounds.

Butyl C4 -C4H9 √

Separation of peptides

and proteins. Shorter

retention than C8, C18.

MOS C8,Octyl -C8H17 √

Less retentive than

C18; normally used for

small peptides and

proteins, pharmaceuti-

cals, steroids, envi-

ronmental samples.

ODS C18 -C18H37 √

Most retentive of the

alkyl-bonded phases.

Used widely for

pharmaceuticals, ster-

oids, fatty acids,

phthalates, environ-

mental etc.

CPS

CN,Cyano,

(propylnitrile)

-(CH2)3CN √ √

Unique selectivity for

polar compounds, more

suitable than base sili-

ca for normal phase

gradient separations.

When used in reversed

phase,the selectivity is

different to that of the

C8 and C18 phases.

Useful for a wide range

of pharmaceutical ap-

plications and for mix-

tures of very different

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solutes.

APS

NH2,

Aminopropyl

-(CH2)3NH2 √ √ √

HILIC: Carbohydrate

analysis and other po-

lar compounds. Weak

anion exchanger, ani-

ons and organic acids.

Normal Phase: Alterna-

tive selectivity to silica.

Good for aromatics.

Phenyl C6H5 -(CH2)C6H5 √

Aromatic compounds

and moderately polar

compounds.

Diol

-(CH2)2O

CH2(CH2O

H)2

√ √

Reversed Phase: Pro-

teins, peptides.

Normal Phase: similar

selectivity to silica, but

less polar.

SCX Strong Cation

Exchanger -RSO3H- √

Organic bases.

SAX

Anion Ex-

changer

Polyeth-

leneimine

(PEI)

-(CH2CH2N

H-)n

Organic acids, nucleo-

tides and oligonucleo-

tides.

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Troubleshooting

Analysis on bad reservation and separation reproducibility

Causes on peak tailing

◇Problems of the process of packing the columns themselves, sieve plate jam or packings col-

lapse.

◇Pollutions on the column head

◇Overloading samples

◇Improper sample solvents

◇Effect beyond columns

◇Chemical or twice reservation effect (Si-OH)

Problems Causes Representations

Differences among different

columns

Different packings and bonded

phases

Retension factor(k), separation

factor(α)

Columns change in the

process of use

Column beds break,

Bonded phase lost,

Silica solved

Strong retension components

stack

Column performance(N)

Retension factor(k), separatin fac-

tor(α),


Retension factor(k), column per-

formance(N)

Extra-column effect Systems differences,Syringe

volume is too big, The tubing

volume between injector, col-

umn and detector is too big,

Flow cell volume is too big,

Dead volume of adapter is too

big.


Change on separation ef-

fect

Change of the mobile phases

components;

Change of flow rate;

Change of temperature

Retension factor(k), small change

of column performance(N)

Retension factor(k), separation

factor(α)

Retension factor(k), small change


Low equilibration Not enough re-equilibration time Retension factor(k), small change


Overload of the column Too much samples Retension factor(k), colulmn er-

formance(N)

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◇Lack of buffers or improper buffers

◇Heavy metal pollutions

How to solve peak tailing problems

A. Peak tailing problems concerned with chemistry

1. Add 30mM triethylamine (for basic compounds) or acetic-amine (for acid compounds), or add ace-

tic-triethylamine for unknowns into mobile phase.

2. If it is still tailing, replace the acetic-triethylamine with dimethyl-octyl amine

3. Make the injection volume less than 1μg.

B. Peak tailing problems concerned with columns

1. Reversed columns can be flushed with 20 times volume solvents: 96%CH2Cl2, 4% CH3OH (methanol),

and 1%NH4OH; normal columns can be flushed with CH3OH (methanol).

2. Use of guard columns.

C. Peak tailing and widening concerned with HPLC system

1. Syringe volume is too big (normally ≤ 25μL )

2. The tubing volume between injector, column and detector is too big (best for tube < 20cm, ID0.007”).

3. The flow cell volume of the detector is too big.

How to store columns

Make the mobile phases in accordance with the actual use to avoid the microorganism produced by

buffered solvents and aqueous mobile phases, or add 200ppm NaN3 to restrain the growth of the bacteria

(Pay attention to its toxicity and potentially explosibility); or add 20% organic modifier in mobile phases

can also restrain the bacteria, and the reconstructive solvents can be helpful to mobile phase degassing.

Use 100% organic solvents (Ethylic-nitrile is best) to store columns instead of buffered solvents.

Columns flushed with buffered solvents should be purged with 15-20 times volume organic mobile

phases without buffers, and then store with 100% organic solvents.

Don’t flush columns bonded C18 with pure water.

Screw with end caps for the both ends of the columns to avoid the packing hardened cracked.

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Guard Columns Selections

As a regular question of chromatography workers, how to select guard columns but not affect separation

analysis always made them think. Usually, make sure the sample is clean before selecting guard column.

For most analytical workers, one 1cm length guard column can fully function for guard use. If samples are

very dirty or often need to change the 1cm guard column, then chose 2cm or 3cm length guard columns.

The longer the guard column, the more packing in, and the less chance the pollutants enter the column,

while the longer of the retention time. Generally speaking, the inside diameter of guard column should be

same with the chromatographic column.

Ways of packing the guard columns are also very important. At present, there is a way of membrane

packing with a very simple and convenient application process, and can be installed in the lab. But the

biggest shortcoming is not economical, especially for China’s current status, for the expense for only one

time use is comparatively high. In addition, the guard columns with the way of membrane have limited

packing materials and provide limited guard function; as these columns are short because of the few

packing materials, they have little effect on retention time of the analytical samples.

Other guard columns are curtate columns, and there are three designs: direct-connection, finger-tight,

and integral. The integral design is that the manufacturers install the guard column together with analyt-

ical column directly according to the order; it’s very convenient but can’t be modified. Direct–connection

design is that the guard columns can be connected to any kind of analytical columns and installed any-

time by workers, besides; the chromatographic workers can select different guard column length in ac-

cordance with samples. The finger-tight design and the direct-connection design are same in structure;

the only difference is it can be installed by hands instead of wrench. In addition, from the structure point of

view, some guard columns can replace their drop-in cartridges, while some others cannot. At present,

most guard columns can replace their drop-in cartridges to reduce the guard columns cost.

Most people select guard column packings in accordance with analytical column packings, normally

speaking, they are same. But they aren’t necessarily same according to actual analytical work.

The principle of selecting guard column is: on the precondition of meeting analysis and separation, select

the shortest guard column and chose packings with the least reservation to samples.

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Moss Series HPLC Columns

Jinhua Moss Analytical Instruments Co., Ltd (JMAIC) is domestic biggest supplier in the field of HPLC

columns, which almost takes up half of columns sales of the whole country, meanwhile, its products also

export to USA, UK, India and other countries and leads a significant role at home and abroad.

JMAIC has a long-term and good relationship with many world famous manufacturers of packings and

columns, so we can meet the needs of a great deal chromatographic workers with good quality and low

price.

To be guaranteed quality, our company is always running under the required of ISO9001:2000 System.

Test every batch of packings before using, and to be strictly with quality control for every column before

leaving factory. Help customers to solve the problem during using by our application lab. Now we have

setted up a good and healthy company image among the customers by our continuous efforts in the re-

cent years.

Our company provides a lot of brands and types packings for analytical columns, fast analytical columns,

gel columns, protein analytical columns, amino analytical columns, guard columns, semi-prep and pre-

parative columns.

Routine Analysis Column

SinoChrom HPLC Columns

SinoChrom ODS-AP

◇ High coverage and exhaustive end-capping

◇ Exceptional batch-to-batch reproducibility

◇ Enhanced mechanical stability

SinoChrom ODS-AP packing featuring maximum surface coverage are the ideal choice for a wide variety

of organic compounds.Carefully controlled full end-capping leads to optimal performance with acidic,

basic and chelating compounds.

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SinoChrom C8

◇ Recommended for higher hydrophobic samples

◇ Versatile reverse phase column

SinoChrom C8 packing materials are bonded with octyl groups and are fully end-capped. They are rec-

ommended for compounds which are too strongly retained on C18 phases.300 Å materials are widely

used for larger molecules with higher hydrophobicity.

SinoChrom C4

◇ Recommended for biological separation

◇ Quick separation of wide hydrophobicity samples

SinoChrom C4 packing materials are bonded with butyl groups and show moderate hydrophobicity. 300A

materials are suited for the separation of peptides and proteins. Especially, larger pore silica with less

surface area is used to avoid denaturation of proteins. The phases are also useful for separating com-

ponents within a wide range of hydrophobicity in one shot.

SinoChrom ODS-BP

◇ Suitable for hydrophilic compounds separation

◇ Strong retention in aqueous condition

◇ Long lifetime in aqueous eluents

◇ Different selectivity from ODS-AP

SinoChrom ODS-BP phases are designed to show extended selectivity for hydrophilic and polar com-

pounds which are either not or poorly retained on other phases. A proprietary modification technique

avoids the matting-down effect of the C18 chains which conventional ODS-phases show at high water

contents in the mobile phase, even if pure water is used, Typical applications are separations of biomol-

ecules and metabolites such as oligosaccharides, amino acids, small peptides, nucleotides and organic

acids.

SinoChrom ODS-BP phases show stable base lines and high sensitivity even under neutral pH conditions

and without buffer or counter-ion additives, which makes them appear especially suited for hyphenated

techniques like LC-MS, where such additives disturb the detect.

In addition, cause of SinoChrom ODS-BP packing is a kind of exhaustive end-capping packings, it has

the same selectivity as routine ODS stationary phase while analysis hydrophilia chemical compounds

with classic reverse phase mobile phase.

SinoChrom APS

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◇ Useful in both normal and reverse phase

◇ Recommended for basic compounds separation

◇ Useful as a support of special modification

SinoChrom APS packing materials are bonded with amino-propyl silane using our ultra pure spherical

silica gel as base material, Depending on the choice of the eluent, it can both be used under normal and

reversed phase mode. Especially, it is useful for saccharide separation using acetonitrile / water, the

phase is also recommended for the separation of basic compounds under normal phase conditions,

which could not be separated using unmodified silica gel. Simple eluents such as hexane / ethyl acetate

or chloroform / methanol are used for the separation without any polar sovent additives. The amine func-

tionality provides higher reactivity and is useful as a support of special modifications such as chiral

phases.

SinoChrom P-ODS

SinoChrom P-ODS is wide range in application. It can absolutely meet with analysis about the samples of

biochemistry, proteins, nucleic acid and so on.

SinoChrom P-NH2

SinoChrom P-NH2 is appropriate for the analysis and separation about the samples of monosaccharide,

disaccharide, sugar alcohol and other saccharides.

SinoChrom P-ODS and SinoChrom P-NH2 are the packing materials based on polymer, which compared

with those materials based on silica are at a wide range of pH 1-14. Recommended used for bad analysis

conditios, which will be a long lifetime.

SinoChrom packings parameters

Phase Particle Size(μm) Surface Area(m2/g) Pore Size(Å) Carbon Load End-capped

ODS-BP 5,10 300 120 15% Yes

ODS-AP 5,10 300 120 17% Yes

C8 5,10 300 120 11% Yes

C4 5,10 300 120 8% Yes

APS 5,10 300 120 4% Yes

Si60 5,10 450 60 - -

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SinoChrom Columns Ordering Guide

Descriptions Particle size（μm） Specification (mm) Part No. List Price (USD )

SinoChrom ODS-BP 5 4.6 x 150 31110004

5 4.6 x 200 31110005

5 4.6 x 250 31110006

5 5.0 x 150 31110404

10 4.6 x 150 31110569

10 4.6 x 200 31110428

10 4.6 x 250 31110008

SinoChrom ODS-AP 5 4.6 x 150 31110001

5 4.6 x 200 31110002

5 4.6 x 250 31110003

5 5.0 x 150 31110648

10 4.6 x 150 31110151

10 4.6 x 200 31110649

10 4.6 x 250 31110650

SinoChrom C8 5 4.6 x 150 31110388

5 4.6 x 200 31110124

5 4.6 x 250 31110125

5 5.0 x 150 31110651

10 4.6 x 150 31110652

10 4.6 x 200 31110653

10 4.6 x 250 31110439

SinoChrom C4 5 4.6 x 150 31110654

5 4.6 x 200 31110655

5 4.6 x 250 31110531

10 4.6 x 150 31110656

10 4.6 x 200 31110657

10 4.6 x 250 31110658

SinoChrom APS 5 4.6 x 150 31110564

5 4.6 x 200 31110664

5 4.6 x 250 31110135

15

10 4.6 x 150 31110665

10 4.6 x 200 31110666

10 4.6 x 250 31110464

SinoChrom Si60 5 4.6 x 150 31110349

5 4.6 x 200 31110351

5 4.6 x 250 31110136

10 4.6 x 150 31110667

10 4.6 x 200 31110668

10 4.6 x 250 31110669

SinoChrom P-ODS 10 4.6 x 150 31110670

10 4.6 x 200 31110671

10 4.6 x 250 31110360

SinoChrom P-NH2 10 4.6 x 150 31110559

10 4.6 x 200 31110672

10 4.6 x 250 31110560

Hypersil HPLC Columns

Hypersil packings parameters


ODS2 5 220 80 11% Yes

C8 5,10 170 120 6.5% Yes

CN 5,10 170 120 4.0% Yes

NH2 5,10 170 120 1.9% Yes

SiO2 5,10 170 120 - -

C1 5,10 170 120 2.5% Yes

C6H5 5,10 170 120 5.0% Yes

SAX 5,10 170 120 2.5% Yes

ODS 5,10 170 120 10% Yes

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Moss Hypersil Columns Ordering Guide


Hypersil ODS2 5 2.1 x 150 31110393

5 4.0 x 150 31110420

5 4.0 x 200 31110171

5 4.0 x 250 31110137

5 4.0 x 300 31110141

5 4.6 x 150 31110027

5 4.6 x 200 31110028

5 4.6 x 250 31110029

5 4.6 x 300 31110030

5 5.0 x 150 31110436

5 5.0 x 200 31110432

5 5.0 x 250 31110332

5 5.0 x 300 31110031

Hypersil ODS (C18) 5 2.1 x 150 31110516

5 4.0 x 150 31110382

5 4.0 x 200 31110475

5 4.0 x 250 31110368

5 4.0 x 300 31110509

5 4.6 x 150 31110018

5 4.6 x 200 31110019

5 4.6 x 250 31110020

5 4.6 x 300 31110021

5 5.0 x 150 31110725

5 5.0 x 200 31110387

5 5.0 x 250 31110551

5 5.0 x 300 31110726

10 2.1 x 150 31110727

10 4.0 x 150 31110728

10 4.0 x 200 31110729

10 4.0 x 250 31110730

10 4.0 x 300 31110731

10 4.6 x 150 31110023

10 4.6 x 200 31110024

10 4.6 x 250 31110025

10 4.6 x 300 31110026

10 5.0 x 150 31110732

10 5.0 x 200 31110733

10 5.0 x 250 31110734

10 5.0 x 300 31110735

Hypersil C8 5 4.0 x 150 31110470

5 4.0 x 200 31110736

17

5 4.0 x 250 31110146

5 4.0 x 300 31110737

5 4.6 x 150 31110032

5 4.6 x 200 31110033

5 4.6 x 250 31110034

5 4.6 x 300 31110140

5 5.0 x 150 31110738

5 5.0 x 200 31110739

5 5.0 x 250 31110740

5 5.0 x 300 31110741

10 4.6 x 150 31110035

10 4.6 x 200 31110036

10 4.6 x 250 31110224

10 4.6 x 300 31110742

Hypersil CN 5 4.0 x 150 31110743

5 4.0 x 200 31110744

5 4.0 x 250 31110566

5 4.0 x 300 31110745

5 4.6 x 150 31110039

5 4.6 x 200 31110040

5 4.6 x 250 31110041

5 4.6 x 300 31110357

10 4.6 x 150 31110180

10 4.6 x 200 31110419

10 4.6 x 250 31110746

10 4.6 x 300 31110747

Hypersil NH2 5 4.0 x 150 31110748

5 4.0 x 200 31110749

5 4.0 x 250 31110496

5 4.0 x 300 31110178

5 4.6 x 150 31110042

5 4.6 x 200 31110043

5 4.6 x 250 31110044

5 4.6 x 300 31110045

5 5.0 x 150 31110750

5 5.0 x 200 31110751

5 5.0 x 250 31110752

5 5.0 x 300 31110753

10 4.0 x 150 31110754

10 4.0 x 200 31110755

10 4.0 x 250 31110756

10 4.0 x 300 31110196

10 4.6 x 150 31110421

10 4.6 x 200 31110046

10 4.6 x 250 31110047

10 4.6 x 300 31110331

18

Hypersil SiO2 5 4.0 x 150 31110461

5 4.0 x 200 31110476

5 4.0 x 250 31110399

5 4.0 x 300 31110565

5 4.6 x 150 31110049

5 4.6 x 200 31110050

5 4.6 x 250 31110051

5 4.6 x 300 31110376

5 5.0 x 150 31110757

5 5.0 x 200 31110208

5 5.0 x 250 31110327

5 5.0 x 300 31110758

10 4.0 x 150 31110759

10 4.0 x 200 31110760

10 4.0 x 250 31110761

10 4.0 x 300 31110366

10 4.6 x 150 31110403

10 4.6 x 200 31110429

10 4.6 x 250 31110177

10 4.6 x 300 31110762

Hypersil C1 5 4.6 x 150 31110335

5 4.6 x 200 31110330

5 4.6 x 250 31110145

5 4.6 x 300 31110763

10 4.6 x 150 31110764

10 4.6 x 200 31110765

10 4.6 x 250 31110766

10 4.6 x 300 31110767

Hypersil C6H5 5 4.0 x 150 31110326

5 4.0 x 200 31110768

5 4.0 x 250 31110588

5 4.0 x 300 31110510

5 4.6 x 150 31110342

5 4.6 x 200 31110409

5 4.6 x 250 31110038

5 4.6 x 300 31110222

10 4.0 x 150 31110384

10 4.0 x 200 31110769

10 4.0 x 250 31110770

10 4.0 x 300 31110642

10 4.6 x 150 31110153

10 4.6 x 200 31110771

10 4.6 x 250 31110363

10 4.6 x 300 31110478

Hypersil SAX 5 4.6 x 150 31110211

5 4.6 x 200 31110333

19

5 4.6 x 250 31110048

10 4.6 x 150 31110579

Hypersil C4 10 4.6 x 250 31110232

5 4.6 x 150 31110238

5 4.6 x 200 31110037

5 4.6 x 250 31110119

Hypersil BDS Packing

◇ Hypersil BDS C18

◇ Hypersil BDS C8

◇ Hypersil BDS CN

◇ Hypersil BDS C6H5

The Characteristic of Hypersil BDS Packing

◇ Outstanding peak shape when analysis alkaline compound

◇ Long lifetime of the column

◇ Better stability

◇ As analysising alkaline compounds, outstanding peak shape when analysis acid compounds

and neutral compounds

Hypersil BDS packings parameters


C18 3, 5 170 130 11% Yes

C8 3, 5 170 120 7.0% Yes

CN 5 170 120 4.0% Yes

C6H5 5 170 120 5.0% Yes

20

Hypersil BDS Columns Ordering Guide


Hypersil BDS C18 5 2.1 x 150 31110350

5 4.0 x 150 31110408

5 4.0 x 200 31110148

5 4.0 x 250 31110369

5 4.0 x 300 31110345

5 4.6 x 150 31110053

5 4.6 x 200 31110054

5 4.6 x 250 31110055

5 4.6 x 300 31110056

5 5.0 x 150 31110489

5 5.0 x 200 31110328

5 5.0 x 250 31110433

5 5.0 x 300 31110772

Hypersil BDS C8 5 4.0 x 150 31110780

5 4.0 x 200 31110781

5 4.0 x 250 31110582

5 4.0 x 300 31110782

5 4.6 x 150 31110060

5 4.6 x 200 31110061

5 4.6 x 250 31110156

5 4.6 x 300 31110783

Hypersil BDS CN 5 4.6 x 150 31110487

5 4.6 x 200 31110169

5 4.6 x 250 31110133

5 4.6 x 300 31110784

Hypersil BDS C6H5 5 4.6 x 150 31110118

21

5 4.6 x 200 31110168

5 4.6 x 250 31110377

5 4.6 x 300 31110789

BETASIL Packings

◇ With highly pure base deactivated silica, offer outstanding peak shape.

◇ High specific surface area and high covered for bonded phase.

◇ With outstanding efficiency, and high theoretical plates.

◇ Strong retention and can be used for analysis of pole-strength compounds.

BETASIL packings parameters

Phase Particle Size(μm) Pore Size(Å) Carbon Load End-capped

BETASIL C18 5,10 100 20% Yes

BETASIL C8 5,10 100 12% Yes

BETASIL C6H5 5 100 11% Yes

BETASIL CN 5 100 6% Yes

BETASIL SiO2 5 100 —— ——

BETASIL Columns Ordering Guide


BETASIL C18 5 4.6 x 100 31110804

5 4.6 x 150 31110086

5 4.6 x 200 31110126

5 4.6 x 250 31110087

YWG PACKINGS (Domestic Packings)

YWG Columns Ordering Guide


YWG C18 10 4.0 x 150 31110805

10 4.0 x 200 31110498

10 4.0 x 250 31110556

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22

10 4.0 x 300 31110806

10 4.6 x 150 31110011

10 4.6 x 200 31110226

10 4.6 x 250 31110012

10 4.6 x 300 31110013

10 5.0 x 150 31110807

10 5.0 x 200 31110105

10 5.0 x 250 31110808

10 5.0 x 300 31110809

Columns used for analysis of protein

Hypersil packings

◇ Hypersil 300 Å C4

Can be used to analysis hydrophobic Oligo-peptide or macromolecular polypeptide.


Can be used to analysis hydrophobic Oligo-peptide or macromolecular polypeptide.


Can be used to analysis hydrophilic or weak hydrophobic Oligo-peptide and protein, or

enzymatic hydrolyzing. Also can be used to analysis various kinds of natural or com-

pound Oligo-peptide.

Hypersil 300Å packings parameters


C8 5,10 80 300 3.3% Yes

C4 5,10 80 300 2.0% Yes

Moss Hypersil 300Å Columns Ordering Guide


Hypersil 300 Å C8 5 4.6 x 150 31110324

5 4.6 x 200 31110412

5 4.6 x 250 31110121

5 4.6 x 300 31110359

23

10 4.6 x 150 31110790

10 4.6 x 200 31110791

10 4.6 x 250 31110518

10 4.6 x 300 31110792

Hypersil 300 Å C4 5 4.6 x 150 31110354

5 4.6 x 200 31110063

5 4.6 x 250 31110064

5 4.6 x 300 31110453

10 4.6 x 150 31110793

10 4.6 x 200 31110355

10 4.6 x 250 31110065

10 4.6 x 300 31110794

SinoChrom Packings

SinoChrom 300Å packings parameters


ODS-AP 5,10 100 300 9.0% Yes

C8 5,10 100 300 5.0% Yes

C4 5,10 100 300 3.0% Yes

SinoChrom Columns Ordering Guide


SinoChrom 300 Å

ODS-AP 5 4.6 x 150 31110116

5 4.6 x 200 31110373

5 4.6 x 250 31110117

10 4.6 x 150 31110536

10 4.6 x 200 31110673

10 4.6 x 250 31110362

SinoChrom 300 Å C8 5 4.6 x 150 31110448

5 4.6 x 200 31110674

5 4.6 x 250 31110234

10 4.6 x 150 31110675

10 4.6 x 200 31110676

10 4.6 x 250 31110677

SinoChrom 300 Å C4 5 4.6 x 150 31110447

5 4.6 x 200 31110678

5 4.6 x 250 31110010

10 4.6 x 150 31110679

24

10 4.6 x 200 31110680

10 4.6 x 250 31110681

Descriptions DAC-50 DAC-80 DAC-100 DAC-150

ID φ 50mm 80mm 100mm 150mm

High of Packed bed 0~400mm

Material of Hardware Kit 316L S. S.

DAC Dynamic Axial Compression Column Ordering Guide

Descriptions

Moss DAC-50 Dynamic Axial Compression Column




Can be customed as required

Semi-prep/Preparative HPLC Columns

The preparative columns packed with high-pressure homogenate

tech have high stable column bed, which makes the preparative

columns have same performance with analytical columns, and

DAC Dynamic Axial Compression Column

Moss DAC Series are new products we launched to the

market used for analysis Industrial Chromatography. To

meet different scale of preparative needing of commercial

proess. Extensive used for the industrial chromatography

systems to analysis natural product, synthetic drugs,

protein and polypeptide bio-pharmaceutical.

25

realize high efficiency separation.

SinoChrom Packings

Ordering Guide

Descriptions Particle size (μm) Specification (mm) Part No. List Price (USD )

SinoChrom ODS-BP 5 10 x 150 31110866

5 10 x 200 31110867

5 10 x 250 31110379

5 10 x 300 31110868

5 20 x 150 31110869

5 20 x 200 31110870

5 20 x 250 31110385

5 20 x 300 31110871

SinoChrom ODS-AP 5 10 x 150 31110611

5 10 x 200 31110872

5 10 x 250 31110873

5 10 x 300 31110874

5 20 x 150 31110875

5 20 x 200 31110876

5 20 x 250 31110877

5 20 x 300 31110878


10 10 x 200 31110389

10 10 x 250 31110110

10 10 x 300 31110176

10 20 x 150 31110395

10 20 x 200 31110231

10 20 x 250 31110111

10 20 x 300 31110356


15 20 x 250 31110468

15 30 x 250 31110688

15 40 x 250 31110689


10 10 x 200 31110693

10 10 x 250 31110573

10 10 x 300 31110444

10 20 x 150 31110694

10 20 x 200 31110695

10 20 x 250 31110172

10 20 x 300 31110101001

26


15 20 x 250 31110468

15 30 x 250 31110696

15 40 x 250 31110697

SinoChrom C8 10 10 x 250 31110700

10 20 x 250 31110701

10 30 x 250 31110702

15 40 x 250 31110703

20 40 x 250 31110705

SinoChrom C4 10 10 x 250 31110707

10 20 x 250 31110708

10 30 x 250 31110709

15 40 x 250 31110710

20 40 x 250 31110712

SinoChrom APS 10 10 x 250 31110714

10 20 x 250 31110715

10 30 x 250 31110716

15 40 x 250 31110717

20 40 x 250 31110719

SinoChrom SiO2 10 10 x 250 31110721

10 20 x 250 31110722

10 30 x 250 31110723

15 40 x 250 31110724

20 40 x 250 31110726

SinoChrom 300 Å ODS-AP 10 10 x 150 31110728

10 10 x 200 31110729

10 10 x 250 31110730

10 10 x 300 31110731

10 20 x 150 31110394

10 20 x 200 31110732

10 20 x 250 31110733

10 20 x 300 31110734

10 30 x 150 31110735

10 30 x 200 31110736

10 30 x 250 31110567

10 30 x 300 31110571

15 40 x 250 31110737

SinoChrom 300 Å C8 10 10 x 250 31110739

10 20 x 250 31110740

10 30 x 250 31110741

15 40 x 250 31110742

SinoChrom 300 Å C4 10 10 x 250 31110744

10 20 x 250 31110745

10 20 x 300 31110746

27

15 40 x 300 31110747

Columns used for Rapid analysis

Ordering Guide

Descriptions Particle size (μm) Specification (mm) Part No. List Price (USD )

Hypersil C18 3 2.1 x 50 31110485

3 2.1 x 100 31110486

3 4.0 x 50 31110521

3 4.0 x 100 31110017

3 4.6 x 50 31110438

3 4.6 x 100 31110229

3 4.6 x 150 31110543

Hypersil C8 3 2.1 x 50 31110795

3 2.1 x 100 31110484

3 4.0 x 50 31110796

3 4.0 x 100 31110797

3 4.6 x 50 31110798

3 4.6 x 100 31110398

3 4.6 x 150 31110601

Hypersil C18 3 2.1 x 50 31110490

3 2.1 x 100 31110435

3 4.0 x 50 31110532

3 4.0 x 100 31110337

3 4.6 x 50 31110592

3 4.6 x 100 31110159

3 4.6 x 150 31110052

Hypersil BDS C8 3 2.1 x 50 31110799

3 2.1 x 100 31110800

3 4.0 x 50 31110801

3 4.0 x 100 31110802

3 4.6 x 50 31110803

3 4.6 x 100 31110804

3 4.6 x 150 31110879

JiajieTM Guard Columns

Moss guard columns are designed with replacement dropin cartridges and packed with imported pack-

ings.

28

Showing from many researches: the polluted column head mainly causes the increase of column pres-

sure, so we put a short column in front end to protect the expensive chromatographic column that is en-

hancing chromatography. Normally speaking, the packings in the guard column are similar to guard

column. Guard column are economical, practical means of extending column lifetime and the column.

JMAIC JiajieTM Guard column designs with convenient, ultra new replaceable drop-in cartridges, so you

only need to replace a new drop-in cartridge when the samples are unclean or start another lab.

Ordering Guide

Descriptions Particle size (μm) Package

(pcs) Part No. List Price (USD )

Guard Column Holders 4.6 × 10mm 1 31110102

C18-Drop-in Guard Cartridges 4.6 × 10mm 1 31110130

4.6 × 10mm 4 31110855

NH2-Drop-in Guard Cartridges 4.6 × 10mm 1 31110157

4.6 × 10mm 4 31110856


4.6 × 10mm 4 31110857

C6H5-Drop-in Guard Cartridges 4.6 × 10mm 1 31110101

4.6 × 10mm 4 31110858

CN-Drop-in Guard Cartridges 4.6 × 10mm 1 31110100

4.6 × 10mm 4 31110859


4.6 × 10mm 4 31110860

BDS C18-Drop-in Guard Cartridges 4.6 × 10mm 1 31110129

4.6 × 10mm 4 31110861


4.6 × 10mm 4 31110862

SAX-Drop-in Guard Cartridges 4.6 × 10mm 1 31110340

4.6 × 10mm 4 31110863

300 Å C18-Drop-in Guard Cartridges 4.6 × 10mm 1 31110540

29

4.6 × 10mm 4 31110864



Silica-Drop-in Guard Cartridges 4.6 × 10mm 1 31110220

Dextrangel Column Special

Dextrangel packing parameters

Phase Particle

Size(μm) Pore Size(Å) Column Bed (mL/g)

Separation Range

pep-

tides/proteins

polysaccha-

rides

Sephadex

G-10 40-120 60 2-3 ＜700 ＜700

Ordering Guide

Descriptions Specification (mm) Part No. List Price (USD )

Sephadex G-10 10 x 300 31110088

15 x 300 31110144

10 x 400 31110334

15 x 400 31110089

10 x 500 31110322

14 x 300 31110092

Columns for Amino Acids Analysis System

30

Ordering Guide

Descriptions Specification (mm) Part No. List Price (USD )

Column for Amino Acids Analysis 4.6 x 250 31110091

Guard column 4.6 x 10

Reagent kit 31990001

Standard substance 18 / 5mg 31990002

HPLC Instruments

Assembly Unit

P1201 HPLC Pump

Flow Range 0.001mL/min-9.999mL/min

increament: 0.001 mL/min

Precision ≤±0.5% (1.000mL/min, 8.5Mpa, wa-

ter, ambient temperature)

Accuracy RSD≤0.1% (1.000mL/min, 8.5Mpa,

water, ambient temperature)

RSD≤0.3% (0.001mL/min-9.999

mL/min )

Max Pres-

sure

40Mpa (0.001mL/min-5.000 mL/min)


Pressure

Precision

≤±3% or 0.5MPa

Pressure

Pulsation

≤1.0%

Rear-piston

cleaner

On-line (optional)

Power AC 110V/220V, 50Hz/60Hz

Consumption 180W

Dimensions 420mm x 300mm x 175mm

■Ordering Details

Item Part No. Price(USD )

P1201 HPLC Pump 31010013

31

P230 HPLC Pump



Precision ≤±0.3% (1.000mL/min, 8.5Mpa, water,

ambient temperature)

Accuracy RSD≤0.1% (1.000mL/min, 8.5Mpa,

water, ambient temperature)

Max Pres-

sure



Pressure

Precision

≤±3%

Pressure

Pulsation

≤1.0%


Consumption 180W


■Ordering Details

Item Part No. List Price(USD )

P230 HPLC Pump 31010003

P230II HPLC Pump 31010012

UV1201 UV/Vis Detector

Wavelength Range 190nm-700nm

Wavelength Preci-

sion

±0.1nm

Wavelength Accu-

racy

±1.0nm

Optical Bandwidth 6nm

Rise Time 0.0s-4.9s

Baseline Noise ±1.0x10-5

AU (empty cell, 254nm,

time: 1.0s)

Baseline Drift ≤1x10-4

AU/h (empty cell, 254nm,

time: 60min)

32

Flow Cell Analytical flow cell: optical path

length 9mm; volume 10μL

Light Source Deuterium lamp


Consumption 110W


■Ordering Details

Item Part No. Price

UV1201 UV/Vis Detector 31020010

UV230+ /UV230II UV/Vis Detector

Wavelength Range 190nm-720nm

Wavelength Preci-

sion

±0.1nm

Wavelength Accura-

cy

±0.5nm

Optical Bandwidth 6nm

Rise Time 0.0s-9.9s

Linear Range ≥1.5AU (5%)


AU (empty cell, 254nm,

time: 1.0s)


AU/h (empty cell, 254nm,

time: 60min)

Flow Cell Analytical flow cell: optical path


Semi-preparative flow cell: optical

path length 3mm; volume 5μL

Light Source Deuterium lamp and Tungsten lamp


Consumption 110W


■Ordering Details

Item Part No. Price

UV230+ UV/Vis Detector 31020004

UV230II UV/Vis Detector 31020009

33

RI230II Refractive Index Detector

Refractive Index Range 1.00-1.75

Detect Range 1/4RIU/FS-5.12 x10-6 RIU/FS

Linear Range 6 x10-4 RIU

Noise ≤2.5x10-9

RIU (STD response)

Auto Zero Range ≥±1x10-4

RIU

Auto Zero Resolution ≥1x10-8

RIU

Drift Adjusting Range 0-6x10-6

RIU

Drift Resolution 3x10-8

RIU

Rise Time FAST, STD, SLOW

Contact Input Purge, Auto zero, Marker, Polar-

ity

Contact Output Intensity alarm, Zero over,

Marker

Temperature Setting 35℃

Cell Volume 8μL

Max Flow Rate 10 mL/min (mobile phase is pure

water)

Max Pressure 0.05MPa

Solvent Contact Parts Stainless steel, Teflon, Quartz

glass


Consumption 60W


■Ordering Details

Item Part No. Price

RI230II Refractive Index Detector 32060089

DAD230+ Diode Array Detector

The DAD230+ Diode Array Detector is the core of powerful and productive systems for routine and occa-

sional users that are easy to use and integrate seamlessly into the modern laboratory. The spectropho-

tometer comes equipped with all the diode-array advantages-fast scanning, excellent sensitivity, virtually

absolute wavelength reproducibility, exceptional ruggedness and reliability, minimum maintenance, and

significant improvements in optical performance.

The DAD230+ Detector has to be used with MAIC’s EC2000 DAD software.

34

Characteristics:

A completely closed optical system and optical fiber based transmission technique for higher spectral

accuracy and stability.

Combined with the powerful data processing system (EC2000 DAD Data System Workstation), the in-

strument provides a full information including chromatogram, spectra of components, three dimension

information and peak purity results.

Wavelength Range 190nm-618nm (512 diodes)

190nm-1015nm (1024 diodes)

Wavelength Preci-

sion

±0.1nm

Wavelength Accu-

racy

±0.5nm

Optical Bandwidth /

Rise Time 0.0s-9.9s

Linear Range ≥1.5AU (5%)


AU (empty cell, 254nm, time:

1.0s)


AU/h (empty cell, 254nm, time:

60min)

Flow Cell Analytical flow cell: optical path length

10mm; volume 10μL

Semi-preparative flow cell: optical path


Light Source Deuterium lamp and Tungsten lamp


Consumption 110W


■Ordering Details

Item Part No. Price

DAD230+ Diode Array Detector (512

diodes)

31020006

DAD230+ Diode Array Detector (1024

diodes)

31020007

35

LU230/LU230II Low-pressure Gradient Unit


Gradient Setting

Range

0.1%-100.0%

Gradient Precision ≤0.2%

Gradient Accuracy ≤2.0%

Gradient Curve Linearity, step, concavo and con-

vex curve

Vacuum Degassing Binary-quaternary (selectable)

Power Requirement AC 110V/220V, 50Hz/60Hz


■Ordering Details

Item Part No. Price

LU230 Low-pressure Gradient Unit 31060005

LU230II Low-pressure Gradient Unit 31060006

LU1201 Low-pressure Gradient Unit 31060010

DG230 On-line Degasser

The DG230 In-line Degasser operates on the principle of Henry’s Law to remove dissolved gases from

HPLC eluent. Henry’s Law states that the mole fraction of a gas dissolved in a liquid is proportional to the

partial pressure of that gas in the vapor phase above the liquid. If the partial pressure of a gas on the

surface of the liquid is reduced, for examples, by evacuation, then a proportional amount of that gas

comes out of solution. The In-line Degasser uses a gas permeable polymer membrane channel to carry

the eluent through the vacuum chamber. When the eluent enters the vacuum chamber, the vacuum

maintains a large differential in gas concentration across the membrane. This accelerates the rate at

36

which the dissolved gases diffuse through the polymer membrane into the vacuum chamber. The gases

are them carried away by the vacuum pump.

Channels 1-5

Max Flow of Single

Channel

10mL/min

Wetted Materials Teflon AF

Degassing Channel

Limited Pressure

4.8x105Pa

Channel Volumn 480μL

Channel Internal Diam-

eter

1.2mm

Pressure Drop 3.9mmHg/mL/min

Power Requirement DC 15V-24V

Dimension 200mm x 150mm x 60mm

■Ordering Details

Item Part No. Price

DG230 On-line Degas-

ser(Duality)

31050001

DG230 On-line Degas-

ser (Quaternion)

31050002

ZWII Column Oven

Temperature Range Ambient plus 5℃-160℃

Temperature Preci-

sion

0.1℃

Accuracy ≤±0.5℃

Temperature Setting 0.1℃

Display 4 digits both for temperature

setting and indication

Power Consumption 145W

■Ordering Details

Item Part No. Price

ZWII Column Oven 31040002

37

AS1201 Auto-Sampler

Precision RSD<0.5%(Full of Sample Loop, or

part of Sample Loop)

Linearity >0.999

Sample residual <0.01%

Number of sample

plates

Two of 60 position 2-mL vial plates

Syringe 250μL(standard), 100μL,

1mL(optional)

Minimum Inject

Volum

0.1μL (250μL Injection Pump)

Sample loop 100μL(Standard); Maximum:5000μL

Switching time of

injection Valve

<100ms

Positional accuracy

of injection Needle

<0.3mm

Mode of Motion X、 Y、 Z (Three-dimensional coor-

dinate)

Injection needle

wash

Wash for inner wall & outer wall of the

needle

Repetition of Sample

Injection

Unlimited

Communication

Mode

LAN/ RS232

Temperature 10 ~ 40℃

Humidity ≤65%, 10~40℃

Pressure 1.01 x 105 Pa

pH Range 1~14

Power AC220V, 50Hz

Dimension 305mm x 470mm x 250mm

Weight 25kg

■Ordering Details

Item Part No. Price

AS1201 Auto-Sampler 31090001

38

RO230/RO1201 Solvent Manager

Channels 2~5

Maxmum Flow rate for

single channel

10mL/min

Material for Channels Teflon AF

Pressure for Channels 0.48MPa

Volume for Channels 480uL

ID for Channel 1.2mm

Pressure Drop 3.9mmHg/mL/min

Dimensions (RO230) 390mm x 280mm x 85mm

Dimensions (RO1201) 420mm x 300mm x 85mm

■Ordering Details

Item Part No. Price (USD )

RO230 Solvent Manager 31080001

RO1201 Solvent Manager 31080002

P230p Semi-Preparative HPLC Pump





Accuracy RSD≤0.2% (10.00mL/min, 8.5Mpa, wa-

ter, ambient temperature)

Max Pressure 30Mpa (0.01mL/min-20.00 mL/min)


Pressure

Precision

≤±3%

Pressure

Pulsation

≤1.0%


Consumption 200W


■Ordering Details

Item Part No. Price

(USD )

P230p Semi-Preparative HPLC

Pump

31010005

39

P270 Preparative HPLC Pump





Accuracy RSD≤1% (30.00mL/min, 8.5Mpa, water,


Max Pressure 30Mpa (1.00mL/min-20.00 mL/min)


Pressure

Precision

≤±3%

Pressure

Pulsation

≤3%


Consumption 260W


■Ordering Details


P270 Preparative HPLC

Pump

31010006

P1000+/P500+ Preparative HPLC Pump

Flow Range P500+ (1~500mL/min, Step 1mL/min)

P1000+ (1~1000mL/min, Step 1mL/min)

Precision ±3% (5Mpa, water, ambient tempera-

ture)

Accuracy RSD≤0.5%

Max Pressure P500+ (0~500mL/min, 20MPa)

P1000+

(0~500mL/min, 20MPa)

P1000+

(>500mL/min, 10MPa)

Pressure

Precision

±5% (0.5MPa)

Temperature 0~40℃

Humidity ≤80%

Power AC 220V ±10%V, 50/60Hz

Consumption 800W


■Ordering Details


P500+ Preparative 31010023

40

HPLC Pump

P1000+ Preparative

HPLC Pump

31010024

P2000+ Preparative HPLC Pump

Flow Range 1~2000mL/min, Step 1mL/min

Precision ±3% (5Mpa, water, ambient tempera-

ture)

Accuracy RSD≤0.5% (1000mL/min, 5MPa, Wa-

ter,R.T.)

Max Pressure 0~1000mL/min, 10MPa

1001~2000mL/min, 5MPa

Pressure

Precision

±5% (0.5MPa)

Temperature 0~40℃

Humidity ≤80%

Power AC 220V ±10%V, 50/60Hz

Consumption 800W


■Ordering Details


P2000+ Preparative

HPLC Pump

31010025

Can be customed by order

41

HPLC System Configurations

Moss iChrom 5100 HPLC System

Isocratic Systems Configurations

Item Description Quantity

1 iChrom P5100 High Pressure Constant Flow Pump 1 ea

2 iChrom D5100 UV/VIS Detector 1 ea

3 iChrom S5100 Auto-Samper (alternative) 1 ea

4 Rheodyne 7725i Sample Injector 1 ea

5 iChrom O5100 Column Oven (alternative) 1 ea

6 iChrom HPLC Column 1 ea

7 iChom W5100 Workstation 1 set

8 iChrom M5100 System Manager 1 ea

Binary High Pressure Gradient Systems Configurations


1 iChrom P5101 Binary High Pressure Constant Flow Pump 1 ea

42







8 iChrom M5101 System Manager (include Binary Degasser) 1 ea

Quaternary Low Pressure Gradient Systems Configurations


1 iChrom P5102 Quaternary High Pressure Constant Flow Pump 1 ea







8 iChrom M5102 System Manager (include Quaternary Degasser) 1 ea

P1201 Isocratic Systems Configurations

P1201 High Pressure Constant

Flow Pump

1 ea

UV1201 UV/VIS Detector 1 ea

Rheodyne 7725i Sample In-

jector

1 ea

SinoChrom ODS-BP Column,

5m I.D.:4.6mm×200mm

1 ea

EC2006 Chromatography

Workstation (Single Channel,

without PC and printer)

1 Unit

■Ordering Details

Item Part No. Price

P1201 Isocratic

Systems

43

P1201 High Pressure Gradient Systems Configurations


Flow Pump

2 ea


Rheodyne 7725i Sample Injector 1 ea

SinoChrom ODS-BP Column, 5m

I.D.:4.6mm×200mm

1 ea

EC2006 Chromatography Work-

station (Single Channel, without PC

and printer)

1 Unit

Gradient Solvent Mixer 1ea

■Ordering Details

Item Part No. Price

P1201 High Pres-

sure Gradient Sys-

tems

P230/P230II Isocratic Systems Configurations

P230/P230II High Pressure Constant

Flow Pump

1 ea

UV230+/UV230II UV/VIS Detector 1 ea



I.D.:4.6mm×200mm

1 ea

EC2000/EC2006 Chromatography

Workstation (Single Channel, without

PC and printer)

1 Unit

■Ordering Details

Item Part No. Price

P230 Isocratic Sys-

tems

P230II Isocratic Sys-

tems

44

P230/P230II High-pressure Gradient System Configurations


Flow Pump

2 ea

UV230+/UV230II UV/VIS Detector 1 ea



I.D.:4.6mm×200mm

1 ea


Workstation (Single Channel, Without

PC and Printer)

1 Unit

Gradient Program Interface 1ea


■Ordering Details

Item Part No. Price

P230 High-pressure Gra-

dient System

P230II High-pressure

Gradient System

Semi-Preparative Isocratic System Configurations

P230p High Pressure Constant Flow

Pump

1 ea

UV230II UV/VIS Detector (Preparative

Flow Cell)

1 ea

Rheodyne 3725i-038 Sample Injector 1 ea

EC2006 Chromatography Workstation

(Single Channel, Without PC and Print-

er)

1 Unit

■Ordering Details

Item Part No. Price

P230p Isocratic System

Semi-Preparative Isocratic System Configurations

P270 High Pressure Constant Flow

Pump

1 ea


Flow Cell)

1 ea




1 unit

45

er)

■Ordering Details

Item Part No. Price

P270 Isocratic System

Preparative Isocratic System Configurations

P1000+/P500

+ High Pressure Constant

Flow Pump

1 ea


Flow Cell)

1 ea




er)

1 unit

■Ordering Details

Item Part No. Price

P500+ Isocratic System

P1000+ Isocratic System

46

Semi-preparative Gradient System Configurations

P230p High Pressure Constant Flow

Pump

2 ea


Flow Cell)

1 ea




er)

1 unit

Gradient Program Interface for

P230p/P270

1ea


■Ordering Details

Item Part No. Price

P230p Gradient System

Semi-preparative Gradient System Configurations

P270 High Pressure Constant Flow

Pump

2 ea


Flow Cell)

1 ea




er)

1 unit

Gradient Program Interface for

P230p/P270

1ea


■Ordering Details

Item Part No. Price

P270 Gradient System

47

Preparative Gradient System Configurations

P1000+/P500

+ High Pressure Constant

Flow Pump

2 ea


Flow Cell)

1 ea


P1000+/P500

+ System Kits 1ea



er)

1 unit

■Ordering Details

Item Part No. Price

P500+ Preparative Gra-

dient System

P1000+ Preparative Gra-

dient System

48

LU230/LU230II Quaternary Low-pressure Gradient System

LU230 low-pressure gradient unit is specially designed for upgrading an isocratic pump LC system into

multi-pumps low-pressure system. With the unit you can conduct mixing and degassing among 2-4 kinds

of solvents, before proceeding with gradient LC analysis.


Flow Pump

1 ea

LU 230 low-pressure gradient unit 1 ea

DG 230-4 in-line degasser 1 ea

UV230+ /UV230II UV/VIS Detec-

tor/DAD230+ diode array detector

1 ea



I.D.:4.6mm×200mm

1 ea


Workstation (Single Channel, Without

PC and Printer)

1 unit

■Ordering Details

Item Part No. Price

LU230 Quaternary

Low-pressure Gradient

System

LU230II Quaternary

Low-pressure Gradient

System

Quaternary gradient system step experiment

with 1% increment

Gradient elution condition for analysis of hop

Item time(min) A/％ B/％ C/％ D/％ Shape

1 0.00 20.0 35.0 10.0 35.0 1.000

2 2.00 20.0 10.0 10.0 60.0 1.000

3 26.00 10.0 0.0 10.0 80.0 0.000

4 35.00 10.0 0.0 10.0 80.0 1.000

5 37.00 20.0 35.0 10.0 35.0 1.000

49

Tests of hop under low pressure quaternary gra-

dient system

Amino Acids Analysis System

MOSS-AAK Amino Acids Analysis System

MOSS-AAK Amino Acids Analysis System adopts chroma-

tographic workstation to accurately control the binary HPLC

high pressure gradient system, and offers accurate flow

speed and mobile phase control. Using pre-column deriva-

tion with 2,4-Dinitro-Fluorobenzene (DNFB) and special

amino acids analysis chromatography column, 18 kinds of

amino acids can be separated distinguishably. A UV/Vis

Detector is normally used for the detection of the amino

acids.

◇ P230 High-pressure Gradient System Configurations

◇ Accessories of Moss-AAK analyze amino acid

Absolutely Necessarily Products Of Analyze Amino Acid

Constant Temperature Water Bath 1ea

ZWII Column Oven 1ea

Diaphragm Vacuum Pump 1ea

Ultrasonic Bench Top Cleaner 1ea

Amino Acid Special Column 1ea

Amino Acid Guard Column 1ea

Box of Amino Acid Reagent 1ea

Amino Acid Standard (18kinds, 5mg/kind) 1ea

φ13mm Nylon (organic) (100/pk) 1pk

φ13mm Cellulose (aqueous) (100/pk) 1pk

Φ13mm Sample Filter (Include Nylon and Cellulose, 200/pk) 1pk

φ50mm Solvent Filter (Include Nylon and Cellulose, 100/pk) 1pk

50

■Ordering Details

Item Ordering No. Price

Amino Acid Special Column for AAK 31110091

Amino Acid Guard Column

Box of Amino Acid Reagent 31990001

Amino acid standard (18kinds, 5mg/kind) 31990002

Constant Temperature Water Bath 33120011

51

MOSS-AAP Amino Acids Analysis System

Standard Configurations

P1201 Amino Acids Analysis System


Flow Pump

2 ea


DG 230-4 in-line degasser 1 ea

ZWII Column Oven 1 ea


Amino Acid Special Column for

AAP

1 ea

Guard Column 1ea

Drop-in Guard Cartridge 2ea

ZJ-1 Vave and Bracket 1ea

TD-1-15 Gradient Solvent Mixer 1ea

EC2006 Chromatography Work-

station

1ea

P1201 Gradient System Kits 1ea

■Alternative for Upgrade

Item Quantity

P1201 Solvent Mixer （include

Binary Degasser）

1ea

AS1201 Auto-Sampler 1ea

52

1. Asp; 2. Glu; 3. Ser; 4. Gly; 5. His; 6. Arg; 7. Thr; 8. Ala; 9. Pro; 10. Tyr; 11. Val; 12. Met; 13Cys

14. Ile; 15. Leu; 16. Phe; 17Lys;

53

Chromatography Data-processing Workstation

EC2006 Chromatography Data-processing Workstation

54

EC2000 Chromatography Data-processing Workstation

EC2006/EC2000 Chromatography data-processing workstation is the new generation chromatography

workstation, with a completely fresh external-connect design hardware and software based on Win-

dows98/XP/2000 dual channels application procedures, with up to date software design technology (O-O)

and the experiences collection of many experts and clients, all of the above make it to be a more func-

tional, and easily-operated workstation.

EC2006/EC2000 is easily operated as MS OFFICE software. Such as, analyze complex samples with

visual and graphical button on line; call out chromatogram with clipboard under the software of WORD

and cerate a report of pictures and words; sent chromatogram by means of E-mail with link procedure;

browse information on internet while not leaving EC2006/EC2000.

EC2006/EC2000 can accommodate any types of chromatography instruments (GC, LC, IC, CE), and is

the ideal substitute of integrator, data processor and traditional workstation, so it will be your right hand in

your laboratory analysis.

EC2000 GPC Chromatography Data-processing Workstation

Based on EC2006/EC2000 workstation, EC2000 GPC adds GC data-processing function. Its characters

are as follows:

◇Standards Calibration

Calculate calibration curve according to molecular weight averages and dispersion coefficient of mono-

disperse or broad standards , to Mark-Houwink (k and a) of narrow or unknown samples; eliminate ex-

periment error by calibrating results of parallel injection of arbitrary number and all kinds standards; re-

gress calibration curve with simple equation, quadratic equation or cubic equation, and auto-calculate

standard error and correlation coefficient; obtain standards average molecular weight and error by re-

versed calculating with fitting curve, and can visually judge and modify fitting results.

55

◇Molecular Weight Determination

By means of molecular weight calibration curve and peak slicing area, calculate number-average molec-

ular weight, weight-average molecular weight, Z average molecular weight, Z+1 average molecular

weight, viscosity-average molecular weight and correlation coefficient of high molecular weight substance;

calculate and analyze any range of the chromatogram by setting up slicing.

◇Molecular Weight Distribution

Calculate to obtain integrated molecular weight distribution curve, differential molecular weight distribu-

tion curve; display distribution plots in the following 3 methods: molecular weight, molecular weight loga-

rithm and logarithmic scale reference frame.

◇Output Results and Printing

Output GC separation chromatogram, calibration curve, integration molecular weight distribution curve,

differential molecular weight distribution curve, various molecular weight and correlation parameters

calculation results; select part or full range to calculate, output, print result with tangent incision method in

accordance with experiment purpose; sent molecular weight results table, molecular distribution plot,

molecular distribution data, calibration curve, standards molecular weight data to clipboard and then

process under Word or EXCEL.

EC2000DAD Chromatography Data-processing Workstation

To guarantee DAD system a stable operation under Windows98/XP2000, we adopt virtual driver. With a

friendly interface and strong function DAD data-processing platform, users can conveniently observe and

process contour map, spectrogram, chromatogram and three-dimensional spectrogram-chromatogram.

With sufficient functions of chromatographic data processing and quantitative calculating, arbitrary re-

volving and parts magnifying, users can obtain more vivid visual effect and results analysis.

DAD workstation can provide all kinds of chromatograms, including single wavelength, arbitrary

56

two-wavelength absorbance, wavelength time procedure, max absorption wavelength and total absorp-

tion, and among them, the most sensitive detection method is max absorption wavelength chromatogram,

while the best method to quantitative reproducibility is total absorption chromatogram.

DAD system provides many discrimination methods on peaks purity, including contour map,

three-dimensional spectrogram-chromatogram, overlap spectrogram, wavelength comparison diagram

and peaks purity calculating method.

Users can conveniently built their ultraviolet spectrogram gallery with the multifunctional spectrogram

gallery managing and searching procedure, and the programme can auto-pick spectrogram to each peak

from spectrogram, chromatogram and three dimensional data; auto-search spectrogram gallery; users

can also add the auto-picked spectrogram into appointed gallery.

■Ordering Details

Item Part No. Price

EC2006 Chromatography Workstation(Double Channel)

EC2000 Chromatography Workstation(Single Channel) 31030001

EC2000 Chromatography Workstation(Double Channel) 31030002

EC2000 GPC Chromatography Workstation(Single Channel) 31030012

EC2000 GPC Chromatography Workstation(Double Channel) 31030013

Waters 510 Gradient Program Interface 18060001

Waters 515 Gradient Program Interface 18060003

P230 Gradient Program Interface 18060019

P270 Gradient Program Interface 18060020

57

Dealers

MOSS is the authorized agent of Themo Fisher Scientific, Shodex, Daicel, TOSHOH, and IDEX (which

includes UPCHURCH, Rheodyne, and I.T.)

Thermo Fisher Scientific (Hypersil columns series)

Shodex columns series

Daicel chiral columns series

TSK-GEL columns series

Upchurch accessories series

Rheodyne injection valves series

58

HPLC Accessories

Tubing and Connections

■Ordering Details

Item Description Part No. Price (USD )

SS316L Stainless Steel Tubing

1/16"O.D. x 0.04"I.D. A210005

1/16"O.D. x 0.03"I.D. A210004

1/16"O.D. x 0.02"I.D. A210003

1/16"O.D. x 0.01"I.D A210002

1/16"O.D. x 0.007"I.D A210001

PEEK Tubing

1/16"O.D. x 0.04"I.D. A310005

1/16"O.D. x 0.03"I.D. A310009

1/16"O.D. x 0.02"I.D. A310004

1/16"O.D. x 0.01"I.D. A310007

1/16"O.D. x 0.01"I.D. A310007

Tefzel (ETFE) Tubing

ID: 1.6mm A310025

ID: 2.4mm A310024

QG-2 Tubing cutters kit

Including 2 wrenches C180011

PEEK Tubing

Including 2 pieces of

blades

C180012

SS316 Stainless Steel Con-

nections

1/16" A460002

1/8" A460012

SS316 Stainless Steel Fer-

rules

1/16" A460001

1/8" A460007

PEEK ferrules

1/16" A460003

SS316L Stainless Steel Union

SS316L Stainless S1teel var-

iable diameter Union

1/16" C170019

1/16"-1/8" C170145

1/8" C180045

59

SS316L Stainless Steel Tee

1/16" C170020

SS316L Stainless Steel Cross

1/16" C170021

TD-1-15 Gradient Mixer



Micro-analytical scale C170060

Analytical scale C170064

Preperative scale C170061

Universal column connections

Patent products C180021

60

Filters

■Ordering Details


SS316L Stainless Steel Sam-

ple Filter

Frit,Ф 13mm C170015

Ф 13mm Cellulose Membrane

0.45 μm,

200pcs/package

C2E0003

Ф 13mm Nylon Membrane 0.45 μm,

200pcs/package

C2E0004

Ф 13mm Cellulose Membrane 0.22 μm,

200pcs/package

C2E0001


200pcs/package

C2E0002

GM Septum Vacum Pump

C2B0009

Solvent Filter

1000mL C2G0003

Solvent Filter 2000mL C2G0004

Solvent Filter Cup C2G0005

Inlet Solvent Flter C2G0006

Solvent Filter Bottle 1000mL C2G0007


0.45 μm,

100pcs/package

C2E0009


100pcs/package

C2E0010

Ф50mm Cellulose Membranee 0.22 μm,

100pcs/package

C2E0011


100pcs/package

C2E0012


0.45 μm, 100

pcs/package

C2B0006

Ф 13mm Nylon Membrane 0.45 μm, 100

pcs/package

C2B0002

Ф 25mm Cellulose Membrane 0.22 μm, 100

pcs/package

C2B0003


pcs/package

C2B0007

61


0.22 μm, 100

pcs/package

C2B0001


pcs/package

C2B0005

Ф 25mm Cellulose Membrane 0.45 μm, 100

pcs/package

C2B0004


pcs/package

C2B0008

On-line Filter

Filter piece

C170014

C20C-402

Solvent Filter

Analytical scale C170051

Preparative scale C170052

AS3120 Ultrasonic Cleaner

AS5150A Ultrasonic Cleaner

PW: 120W, Volume: 3L C2F0001

Volume: 6L C2F0003

62

Syringes

■Ordering Details


Home-made Syringes

10 μL, for 7125, 7725 syringe C2C0002

25 μL, for 7125, 7725 syringe C2C0003

50 μL, for 7125, 7725 syringe C2C0004

100 μL, for 7125, 7725 sy-

ringe

C2C0005

Hamilton Syringes

10 μL, for 7125, 7725 syringe C2A0001

25 μL, for 7125, 7725 syringe C2A0002

50 μL, for 7125, 7725 syringe C2A0003

100 μL, for 7125, 7725 sy-

ringe

C2A0004

250 μL, for 7125, 7725 sy-

ringe

C2A0005

500 μL, for 7125, 7725 sy-

ringe

C2A0006

SGE Syringes

5 μL, for 7125, 7725 syringe C290005

10 μL, for 7125, 7725 syringe C290001

25 μL, for 7125, 7725 syringe C290002

50 μL, for 7125, 7725 syringe C290006

100 μL, for 7125, 7725 sy-

ringe

C290004

250 μL, for 7125, 7725 sy-

ringe

C290003

500 μL, for 7125, 7725 sy-

ringe

C290007

Home-made Special

Injection Needle

For Rheodyne 25 series in-

jectors

C2Z0011

63

Columns Accessories

■Ordering Details


Hardware Kits

ID: 4.6mm, L: 150mm AB15463020

ID: 4.6mm, L: 200mm AB20463020

ID: 4.6mm, L: 250mm AB25463020

JiajieTM Empty

Guard Column

OD:8mm, L:28mm B150176

Frit Sheet

ID: 4.6mm A450120

Seal Fittings

Blue A450115

Variable connector

for precolumn and

analytical column

C180002

Fixed connector

for precolumn and

analytical column

C180001

64

Instruments Accessories

■Ordering Details


ZJ-1 Holder, for injection in-

jector and column

C170023

ZJ-2 Holder, for Rheodyne 25

series injectors

C170080

ZJ-3 Holder, for Rheodyne

switching injectors

C170031

Solvent Bottle (colourless)

100mL

250mL C2Z0001

500mL C2Z0002

1000mL C2Z0003

2000mL C2Z0004

Solvent Bottle (dark brown) 250mL C2Z0048

500mL C2Z0047

1000mL C2Z0049

FL-1 Splitter

Variable C170031

JC200-1 Flow Cell

JC200-2 Flow Cell

8μL, UV200II, Ana-

lytical

C170041

3μL, UV200II, Ana-

lytical

C170043

Deuterium Lamp

Moss UV200II C2J0004

Moss UV230+ C2J0006

Waters 484 C2K0060

Waters 486 C2K0058

Waters 2487 C2K0059

Agilent HP-1100 C2K0057

Shimadzu SPD-10A C2K0061

Linear 100(LabAlliance

Model 500,TSP-100)

C2K0062

Thermo SP8800 C2K0063

PE LC-200 C2K0064

Hitachi/Merck L3000,4000 C2K0065

65

Jinhua Moss Analytical Instruments Co., Ltd.

No. 5 Hepanxiang, Jinhua, Zhejiang 321001 China

Tel: 86-579-8237 3070

Fax: 86-579-8353 3099

http://www.elitehplc.com/

mailto:[email protected]

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