Citotoxicity  on  human  conjunc/val  cell  cultures  of  BAK  preserved  travoprost  and  travoprost  

with  Polyquad®. Pérez-Roca F.(1) Rodrigo-Morales E(2),. Ramos-López JF (2)

1.- Hospital de Baza, Baza Spain  2.-Hospital Universitario Virgen de las Nieves, Granada Spain

To study the cytotoxicity effects on human conjunctival cells by travoprost with benzalkonium chloride as preservative (BAK) and travoprost with Polyquad® a polycationic polymer as a novel preservative used in glaucoma medical treatment

Our   results demonstrated that both travoprost with BAK and travoprost with polyquad® produce a dose-dependent toxicity effects. Although, travoprost with polyquad® appears to be less harmful in the cultured human conjunctival cells maintaining a higher metabolic rate were higher concentrations were used in all times of ex vivo exposure with significant differences between them (p<0.05)

This work demonstrated that travoprost with Polyquad®, a novel preservative used in glaucoma medical treatment, has lower ocular toxicity in human conjunctival cells ex vivo.

Introduction

Methods  

Conclusion  

In one hand, primary cell cultures of human conjunctival cells were stablished from surgical biopsies of patients without ocular surface disease. In another hand, we investigated the cytotoxic effects by sequential dilutions of travoprost with BAK and travoprost with Polyquad®. For these aim, WST-1 assay, a non-radioactive colorimetric quantitative method that measure mitochondrial enzyme activity were performed at 5 minute of exposure.

Results  

P5.117  

0  

20  

40  

60  

80  

100  

120  

CM   0,10%   1%   5%   25%   50%   75%   100%   CT  

WST-­‐1  5  min  Travoprost  

TRAVOPROST-­‐BAK   TRAVOPROST-­‐POLYQUAD  

ConjuncCval  cells  on  culture