citrus research and education foundation field day
TRANSCRIPT
CREF Field Day, March 7, 2012 Page 1
Introduction – Arnold Schumann and Jackie Burns, CREC
The 180-acre citrus grove at Lake Placid is being subleased by the Citrus Research and
Education Foundation (CREF) from the Florida Fish and Wildlife Conservation Commission.
http://myfwc.com/viewing/recreation/wmas/lead/lake-wales-ridge/visitor-information/
The grove is located at an altitude of 60-75 feet above mean sea level, and has two soil series;
Satellite sand and Archbold sand. The original trees of ‘Hamlin’ and ‘Valencia’ oranges were
established in about 1990, most on Swingle rootstock. From 1999 to 2001 the grove was
CREF Field Day, March 7, 2012 Page 2
abandoned, and the subsequent successful rehabilitation process by the CREC, with help from
Lykes Bros. and others, brought it back to a profitable production level by about 2004, just as the
first canker infections were discovered in the NE block. The first HLB infected trees were
identified in 2007, although the disease was probably already established in the NE corner of the
grove in 2006.
From 2007 until 2009 the grove was surveyed quarterly and HLB-symptomatic trees were
removed and destroyed. Psyllids were controlled with frequent insecticide sprays, but the
intensity of sprays was further increased to about the current 9 per year when it became apparent
that the spread of HLB in the grove was unchecked (Fig. 1). By September 2009, the cumulative
infection was about 8% and further removal of infected trees was stopped because at the
exponential rate of infection observed, we anticipated rapid loss of fruit harvests and economic
collapse of the grove was imminent. From spring 2010 until present the grove has been managed
with a more intensive soil- and foliar- applied fertilizer program (see Table 2 in the additional
Figure 1. Cumulative HLB infection in the entire CREF grove at Lake Placid
CREF Field Day, March 7, 2012 Page 3
information section), more aggressive psyllid control program, and only unproductive trees are
being removed – most of them due to blight infection. The current average HLB infection level
in the grove is estimated to be >80% (Figs. 1 & 2).
Figure 2. Block layout and area (acres, lower number) in the CREF grove (left), and spatial distribution of
HLB infections (right). Blue dots represent the HLB infected trees identified in 2011.
CREF Field Day, March 7, 2012 Page 4
The grove blocks 30, 32 and 27b are planted with mature ‘Hamlin’ orange, the new block 31 is
1-year old ‘Vernia’ on three different rootstocks, and the rest of the grove is planted with mature
‘Valencia’ orange. The yield history for the two main varieties in the grove is shown in Fig. 3,
with an apparent upward trend from 2009/10 to 2011/12. The average ‘Hamlin’ yield for this
harvest season was 514 boxes/acre.
The CREF grove is in a very cold location during winter cold front episodes, being on the
windward side of Lake Istokpoga during a typical freeze night with a north-westerly wind.
During the winter of 2010/11, the air temperature was less than 28 degrees Farenheit for more
than 9 hours during two nights, and for shorter periods on other nights. Fruit losses due to
Figure 3. Average fruit yield of ‘Hamlin’ and ‘Valencia’ in the CREF grove.
CREF Field Day, March 7, 2012 Page 5
freezing, and defoliation of trees was considerable, as shown in photos in the “additional
information” section. Fortunately the affected trees were able to recover and produce the good
crop of fruit we now see in 2012.
The research topics in the program are part of a comprehensive USDA/SCRI block grant entitled
“Management strategies for Huanglongbing-infected groves: decision-making assistance for
the Florida citrus grower”. G.P. Solutions (Labelle, FL) contributed major in-kind support in
the form of their foliar nutrition products which were used in 2010 and 2011 seasons for the
entire grove. The grant funding started in 2009/10 and research results to date are all part of
ongoing work in progress.
Leafminer Research Update – Lukasz Stelinski, CREC
CLM Control with Mating Disruption (Fig. 4):
• Mating disruption of CLM was highly effective for periods of 8 weeks or longer
• Proven effectiveness has been limited to mature groves thus far at the commercially
available rate
• Leaf infestation is reduced prior to mating; therefore, it is a proactive control tool
• Reductions in infestation in grapefruit have been correlated with significantly reduced
levels of canker infection
• Highly pest specific; has no impact on natural enemies or on other pests such as psyllids
• Can be applied by hand or by machine; currently machine application requires
specialized equipment
• Machine application can be contracted in coordination with the company selling the
product
Insecticide resistance monitoring and management (Fig. 5):
• The development of insecticide resistance may become a major obstacle to the successful
management of the Asian citrus psyllid (ACP) if steps to prevent it are not taken now
• The baseline susceptibility data for both adult and immature ACP to commonly used
insecticides were collected for five ACP populations from various parts of Florida from
2009-2011.
• Increased activity of insecticide detoxifying enzymes has been correlated with insecticide
resistance in ACP.
• Five CYP4 genes have been identified that are implicated in development of resistance in
ACP.
• ACP populations in Florida exhibit some level of decreased susceptibility to all registered
insecticides used for their management.
• From 2010 to 2011, resistance levels to two important classes of insecticides (pyrethroids
and organophosphate) increased dramatically for certain Florida populations of ACP.
• Resistance levels have not increased for the neonicotinoids from 2010 to 2011 (Good
news!)
CREF Field Day, March 7, 2012 Page 8
Highlands County Citrus Health Management Areas for Coordinated Area
Sprays – Tim Hurner, Highlands County Extension
Highlands County has Seven Citrus Health Management Areas (CHMAs)
The Highlands CHMAs and their Area Captains are:
Avon Park/Lake Letta CHMA
Lake Letta/Sebring CHMA
Bairs Den CHMA
Area Captain: Steve Farr – Ben Hill Griffin (863) 635-2251
Central Highlands 17/27 CHMA
Area Captain: John Gose – Lykes Brothers (863) 465-4127 [email protected]
East 70 Flatwoods CHMA
Lorida CHMA
Area Captain: Doug Schobert – Premier Citrus (772) 562-5030
Kissimmee River Basin CHMA
Area Captain: Tommy Todd - Lykes Citrus (863) 467-1384 ext.302
Highlands CHMA Psyllid Survey 1/6 – 2/3/2012
CHMA Area Acres* Blocks* Psyllid Count
Avon Park/Lake Letta 2,297 114 98
Lake Letta/Sebring 1407 68 19
Central Highlands US 27 1953 88 164
Bairs Den 4144 187 155
East 70 Flatwoods 398 20 66
Kissimmee Basin 4189 71 67
Lorida 2397 59 10
*Surveyed area
Upcoming CHMA Coordinated Area Spray
The next Coordinated Area Spray will occur in the last half of March or the first week in April
depending on the end of bloom, new flush, and psyllid populations. The chemical will be an O.P.
To be sure you are coordinating with your neighbors; contact your Area Captain to get a
notification of the exact timing of the spray for your CHMA Area.
Go to the CHMA Tool Kit on the CHMA Web Site http://www.flchma.org for a list of
recommended Organophosphates
CREF Field Day, March 7, 2012 Page 10
History of Corymbia torelliana as a Windbreak and Cultural Guidelines - Bill
Castle, Professor Emeritus, CREC
Name: Corymbia torelliana, commonly referred to in Florida as just “torelliana” is related to
Eucalyptus, in fact is was originally known as E. torelliana. Recent studies resulted
in it being placed in its own genus: Corymbia.
Origin: The plant is native to Australia where it is used for land conservation and other
purposes.
Florida History: As a windbreak in Florida, the plant began its life with Chuck Obern, a
vegetable grower west of Clewiston. He planted the species around his property and
internally for wind protection. His trees were mature during the 2004 and 2005
hurricane seasons. One hurricane passed nearby and the torelliana plants sustained
little damage. The performance of this plant at the Obern farm attracted enough
attention to warrant trialing it as a windbreak for canker management in citrus groves.
Characteristics: Fast-growing (they can easily reach 10-12 feet in height in the first full year of
growth under good conditions); relatively large leaves; retain lower branches;
spreading canopy. Are they salt and cold tolerant? Yes, however, their cold
tolerance is still being evaluated. Most evidence so far suggests that they are
considerably more tolerant after one year of good growth. Young trees in colder
locations may not survive. Are they tolerant of calcareous sites? Yes.
Windbreak design: The purpose of a windbreak is to slow the wind, not to establish a wall and
completely block the wind. Therefore, it is not recommended to plant trees closer
than 8 ft. in the row nor more than 12 ft. between trees. Spacing between trees and
the specific design of the windbreak are important economic considerations. The
spacing between trees relates directly to cost as does the number of rows. The latter
is dictated by cost and available space. The torelliana windbreak can be established
as a single row. Some double offset rows have also been planted with rows about 8
ft. apart; however, there is the view that land planted to a windbreak is land not
planted to citrus trees, so devoting up to 15% of flatwoods land to a windbreak is not
acceptable!
Site preparation/tree planting:
Prior to planting, clean the row(s), treat them with a residual herbicide and
incorporate organic matter. This is critical. Nothing assures a good first year
better than site preparation and conducive cultural practices.
Install irrigation. This is critical to insure the best growth possible in Year 1.
Plant in the spring to provide a full growing year. This is critical.
Use nursery plants about 2 ft. tall.
Cultural management:
Irrigate the windbreak plants as if they were citrus trees.
Fertilize the trees regularly with a balanced material the first year or two with
slow-release, dry or liquid.
Weed management may or may not be needed. The basic strategy is to support
the fullest expression of the plant’s vigor and, thus, any weed pressure would be
minimized.
CREF Field Day, March 7, 2012 Page 11
Torelliana plants appear to be quite accepting of, responsive to, mechanical
hedging and topping.
Expectations:
Torelliana plants appear to be broadly suitable for Florida grove site conditions,
but they are responsive to good cultural practices.
The plants are propagated as seedlings. That means the plants display some
variability, i.e., there will be some odd or different plants in the windbreak. Good
WB management probably involves replacing those plants to maintain the
integrity and function of the WB.
Variability is also introduced because of different site conditions related to soil
and drainage. Excellent site preparation, especially adding organic matter, helps
minimize negative site effects.
A double-row WB costs more, but it is a good form of insurance against “holes”
in the WB.
NOTE – Dr. Don Rockwood, Professor Emeritus, School of Forest Resources and
Conservation, has established seed orchards of Torelliana and selected potentially
superior plants. Nursery plants are available.
Information resources:
CREC windbreak website. http://www.crec.ifas.ufl.edu/extension/windbreaks/.
EDIS. http://edis.ifas.ufl.edu/.
o Potential Woody Species and Species Attributes for Windbreaks in Florida.
http://edis.ifas.ufl.edu/fr286.
o Field Guide to Identify the Common Casuarina (Australian Pine) Species in
Florida. http://edis.ifas.ufl.edu/hs394.
o The Benefits of Windbreaks for Florida Growers.
http://edis.ifas.ufl.edu/fr253.
o Management of Field Windbreaks. http://edis.ifas.ufl.edu/fr290.
o Fundamentals of Citrus Canker Management. http://edis.ifas.ufl.edu/pp153.
o Windbreak Designs and Planting for Florida Agricultural Fields.
http://edis.ifas.ufl.edu/fr289.
Advanced Citrus Production Systems (ACPS) – Arnold Schumann, CREC
The third objective of the USDA/SCRI block grant proposal was to “evaluate an advanced
production method for replanting in HLB-endemic groves”. The selected site in the northeastern
side of the grove was ideal for the ACPS because both canker and HLB were first found there
(Fig. 7). The previous ‘Hamlin’ block on the site was destroyed by severe freezes during the
period when the grove was abandoned. The 5.6-acre experiment block was designed primarily
for evaluating and demonstrating different citrus rootstocks and a rooted cutting propagation
method, delivery of fertigation by microsprinkler or drip, and use of controlled release fertilizer
(CRF) (Figs. 7-8). The windbreak trees planted around the block are to help protect the young
citrus trees from rapid infection by canker from the adjacent grove (Fig. 7).
CREF Field Day, March 7, 2012 Page 12
Figure 7. Layout and design of the ACPS experiment. Green dots are trees with drip fertigation, pink dots
are trees with microsprinkler fertigation and blue dots are trees with microsprinkler irrigation and CRF.
Yellow dots are the Torrelliana windbreak trees.
Figure 8. Location of ‘Vernia’ orange on three rootstocks and location of ‘Valencia’
orange rooted cuttings in full rows of the experiment.
CREF Field Day, March 7, 2012 Page 13
The ‘Vernia’ trees were planted out in March 2011 and the ‘Valencia’ rooted cuttings in October
2011. The trees are therefore too young to display meaningful differences at this time and will be
reported on periodically in future to update readers and visitors.
The ACPS block represents a useful prototype design for growers wanting to try out the
technology at a reasonable cost and small scale. Please refer to Figure 9 to identify the main
components which will be discussed during the field day.
Figure 9. Requirements for a small ACPS installation showing labeled
components outside (top) and inside (bottom) the utility shed.
CREF Field Day, March 7, 2012 Page 14
HLB – Tim Spann, CREC
A foliar nutrient spray and fertigation experiment was initiated in early 2010 (Fig. 10) to
compare additional intensive fertilization methods with the conventional dry granular fertilizer
program and standard supplementary micronutrient sprays in HLB-endemic situations. The
objective of this study was to determine the profitability of aggressive HLB-infected tree removal
versus enhanced grove care to sustain production.
In 2010 the cumulative HLB incidence was >20%, and by the end of 2011 it was >80% (Fig. 1).
Large plots of 84 trees each in the 38-acre block were chosen in order to obtain better averaging
of measured parameters across the highly variable HLB infection severity typically seen in
infected groves.
*The high foliar nutrition program included major, secondary and micronutrients applied with
every pesticide spray (see table 2 in additional information section). The plant nutrient elements
used were N, P, K, Ca, Mg, S, Mn, Zn, Mo, B, Fe. Non-nutrient active ingredients were
phosphite as potassium phosphite, salicylic acid as potassium salicylate, and Bacillus subtilis
biofungicide.
Figure 10. Plot layout and experimental procedures for the foliar nutrition / fertigation experiment*.
CREF Field Day, March 7, 2012 Page 15
The standard foliar nutrition program consisted of 1-2 annual applications (April and June,
coinciding with leaf flushes) of Mn, Fe, and Zn at labeled rates of 2 gal/acre (Growers Fertilizer
Corporation liquid 1.6% Fe, 2.0% Fe, 2.0% Zn). The scouting, removal treatment was not
continued after 2010 due to the very high HLB infection rates which would have required
destruction of nearly 25% of the block. We also acknowledged the difficulty of conducting
representative inoculum removal treatment comparisons within the block, without the ability to
block psyllid movement across treatments. Granular fertilizer was applied at a nitrogen rate of
200 lb/acre, split equally four times per year. Calcium nitrate was used as the nitrogen source
when available, or else ammonium nitrate was used. In 2010 calcium nitrate was used in
February and April, while in 2011 it was used in every application. The composition of the
granular fertilizer in 2011 was 10-02-13 + 11.3% Ca, 1.7% Mg, 3.5% S, 0.03% Fe, 0.03% B.
Supplementary fertigation was applied during the early spring and summer to the replications in
the eastern half of the block (Fig. 10). Comprehensive hydroponics nutrient solution (see Figure
12 in the additional information section) at 0.5 lb N /acre equivalent was injected in the daily
irrigation, using an automated Netafim controller. About 20 lb/acre additional nitrogen was
added to the annual total in the fertigated half of the block.
Other horticultural practices were uniform for the experiment block, including daily automated
irrigation, herbicide application, mowing, hedging/topping, and the intensive psyllid control
sprays applied in conjunction with the local CHMA (see table 2 in the additional information
section). Due to the inability to irrigate the entire block at the same time, no irrigation water was
applied for freeze protection. During the winters of 2009/10 and 2010/11 quite severe freeze
damage to fruit and foliage occurred in the block, resulting in a partial yield loss for the 2010/11
season. This 2011/12 winter season was milder and the yield will be estimated and statistically
analyzed.
Four to six-month old spring-flush leaves were collected and analyzed in July each year to assess
the nutritional status of the trees in general and to determine the treatment responses (Table 1).
Most nutrient elements were in the recognized sufficiency ranges except for low concentrations
of manganese (Mn) in treatment 2. The iron concentrations were also in the slightly low range
for the whole citrus block, which matches with some of the leaf nutrient deficiency symptoms
visible in the grove. Significant treatment differences were only detected for leaf P and Fe
concentrations, where values were slightly elevated in the treatment receiving the intensive
nutrient and phosphite sprays.
The results of two HLB disease surveys conducted in the experiment during September 2010 and
March 2011 were analyzed statistically. Cumulative infection levels in the experiment block
were about 35%, but no statistically significant difference could be detected between the
treatments (35.6% and 34.7% for the intensive foliar nutrient spray and standard control
treatments, respectively).
CREF Field Day, March 7, 2012 Page 16
Table 1. Nutrient concentrations of ‘Valencia’ orange leaves in July 2010
Treatment
N P K Mg Ca S B Zn Mn Fe Cu
(% DW) (mg/kg DW)
(2) Control 2.72 0.129 1.11 0.531 3.23 0.334 74.4 30.3 18.8 41.6 34.3
(1) Nutrients+SAR 2.78 0.138 1.21 0.514 3.17 0.322 74.8 33.2 20.2 44.8 33.8
LSD(0.05) 0.102
NS
0.008*
0.125
NS
0.025
NS
0.256
NS
0.033
NS
5.51
NS
4.79
NS
4.21
NS
2.68*
4.32
NS
Citrus nutrition field experiments are generally slow to respond to treatments. At this early stage
of two years since treatments were imposed, there are no apparent differences in tree
performance between the standard and intensive foliar spray + fertigation programs, although the
2011/12 fruit yield has yet to be picked and analyzed.
The experiment might show treatment differences
given enough years, but non-significant results to date
suggest that a comprehensive granular fertilization
program may sustain trees in HLB-endemic groves
without the need for elaborate foliar spray programs.
There is thus scope to rationalize foliar sprays, but
they are a good insurance for situations where the
nutrient supply from root uptake is compromised.
Instead of heavy applications of multiple nutrients at
the same time in sometimes incompatible
combinations, it may be more effective biologically
and economically to apply foliar nutrients separately
at strategic times during the year (pre-bloom, post-
bloom, leaf flushes) to supplement the ground
fertilization program. There are many environmental
and management factors which interact with the HLB
disease complex in a grove. The automated daily
irrigation used in the CREF grove may also be
beneficial by reducing stress on HLB-infected trees,
helping them to survive and continue producing fruit.
Figure 11. Walk-through path for field tour
CREF Field Day, March 7, 2012 Page 17
Additional Information
Spray and fertilizer programs (Where trade names appear, no discrimination is intended, and no
endorsement by the University of Florida is implied)
Tab
le 2
. pes
tici
de
and
inte
nsi
ve f
olia
r n
utr
itio
n p
rogr
am f
or t
he
blo
ck 2
9 ex
per
imen
t 2011
Month
Timing
Product
Rate
/100gal
Foliar nutrients
Pests
Comments
Jan
About mid
month for
urea
Dimethoate 4E
LI 700
1 pint/A
0.25% v/v
Low
biu
ret u
rea:
3.2
6% c
once
ntra
tion
(use
27
lb u
rea
in 1
00 g
allo
ns w
ater
)
ACP
LI 700 added to
buffer pH to 5.5-
6.5
mid Feb
Just prior
to bloom
Danitol
16 oz/A
So
lub
or
(use
1 lb
in 1
00 g
allo
ns w
ater
) C
alci
um
nit
rate
– s
pra
y gr
ade
(use
5 lb
in
100
gallo
ns w
ater
) –
or 9
0 oz
CN
9
ACP
Use 2 lb CN in
aerial spray of 15
gpa (or 36 oz CN9)
Mar/Apr
Avoid pesticides
during bloom
N
itro-
30 S
RN
1 g
al/A
+ R
ecov
erR
X 3
-18-
18
2gal
/A +
Mic
rote
ch A
G 2
qts/
A +
TK
O 0
-29-
26 1
qt/A
+ C
ompa
nion
2-3
-2 1
qt/
A
Apr
Immediately
after bloom
Lorsban
5 pint/A
Mic
rote
ch A
G 2
qts/
A +
8oz
/A S
AR
Act
ivat
or
SA
+ C
ompa
nion
2-3
-2 1
qt/A
ACP
May
Early May
Mustang
4.3 oz/A
Mic
rote
ch A
G 2
qts/
A +
8oz
/A
SA
R
Act
ivat
or +
Com
pani
on 2
-3-2
1 q
t/A
ACP
Jun(1st
summer
oil)
End May to
1st week
June
435 oil
Movento
Delegate
3% v/v
10 oz/A
4 oz/A
Nitr
o-30
SR
N 1
gal
/A +
Rec
over
RX
3-1
8-18
2g
al/A
+ M
icro
tech
AG
2qt
s/A
+ T
KO
0-2
9-26
1qt
/A +
Com
pani
on 2
-3-2
1 q
t/A
Foliar
fungal
diseases
ACP, CRM,
scales
CLM,ACP
Jul(2nd
summer
oil)
End of July
435 oil
Imidan
LI 700
2% v/v
1 lb/A
0.25% v/v
Nitr
o-30
SR
N 1
gal
/A +
Rec
over
RX
3-1
8-18
2g
al/A
+ M
icro
tech
AG
2qt
s/A
+ T
KO
0-2
9-26
1qt
/A +
Com
pani
on 2
-3-2
1 q
t/A
Foliar
fungal
diseases
ACP
LI 700 added to
buffer pH to 5.5-
6.5
Aug
No spray unless
ACP populations
high
Sep
Early Sep
Actara 25WG
435 oil
4 oz/A
2% v/v
Mic
rote
ch A
G 2
qts/
A +
8oz
/A
SA
R
Act
ivat
or
SA
+ C
ompa
nion
2-3
-2 1
qt/A
+
Cal
ciu
m n
itra
te –
spr
ay
grad
e (u
se 5
lb in
10
0 ga
llons
wat
er) –
or 9
0 oz
CN
9
ACP
Foliar
fungal,
CRM
Oct
Mid Oct
Danitol
16 oz/A
Cal
ciu
m n
itra
te –
spr
ay
grad
e (u
se 5
lb in
10
0 ga
llons
wat
er) –
or 9
0 oz
CN
9
ACP
Use 2 lb CN in
aerial spray of 15
gpa (or 36 oz CN9)
Nov
No spray unless
ACP populations
increasing
CREF Field Day, March 7, 2012 Page 18
*Where trade names appear, no discrimination is intended, and no endorsement by the
University of Florida is implied.
Figure 12. Comprehensive hydroponics fertilizer used for supplementary fertigation of mature trees and full
fertigation of young trees*
CREF Field Day, March 7, 2012 Page 19
Management of HLB disease in Maury Boyd’s Orange Hammock grove, 2006 – 2012
Tim Spann, CREC
Efforts to manage HLB affected trees with an intensive nutritional program has become the norm
for many growers. Such a program is only sustainable if the trees continue to produce a quality
crop of fruit. However, when many Florida growers began to seriously look at a nutritional
program as an option there was little data available to quantify the effects of HLB on yield
decline and there was no data about how infected trees performed on a nutritional program.
Beginning in the 2008/09 harvest season we began to collect these data.
Our objective was to track HLB symptomatic and healthy – that is trees in the same grove with
no detectable HLB visually or by PCR – trees of both Hamlin and Valencia varieties over time to
see how they performed on a nutritional management program. At the time the project began
most growers were still removing infected trees so the project was begun in the Orange
Hammock grove in Felda, FL belonging to Maury Boyd. We selected 10 trees of each status
(healthy and HLB symptomatic) for each variety. For Hamlin, the trees were selected from
within two adjacent beds; for Valencia the trees were selected within a single bed. Each year at
harvest the trees are completely stripped and the fruit from each tree are analyzed using the
sizing line in the packinghouse at the CREC. Each individual fruit is weighed and the average
diameter is recorded; a total number of fruit per tree is also calculated.
During the first year, we did a detailed sorting, by hand, of the fruit on the symptomatic trees.
From this we determined that > 95% of all symptomatic fruit (small, lopsided) on symptomatic
trees were less than 2.4 inches in diameter. Thus, by observing the percentage of fruit on a tree
each year that are less than this size we can see if the tree is producing more or less symptomatic
fruit over time.
The tables show some of the data that we have collected to date. Not surprisingly, the HLB
affected trees consistently yield less than the healthy trees and these differences are statistically
significant. However, depending on the year, HLB trees have yielded between about 1.5 boxes
per tree to over 3 boxes per tree – after at least 5 years of known disease infection. Also not
surprising, the HLB affected trees have a greater percentage of fruit < 2.4 inches in diameter, but
this too fluctuates seasonally. Note that the there are fruit on healthy trees < than 2.4 inches;
these are not HLB-symptomatic, but are naturally small fruit.
Statistically, when these data are analyzed, there is no significant interaction between tree health
and year for either yield or percentage of small fruit. This means that the changes that occur from
year-to-year are independent of those changes caused by HLB. Put another way, healthy and
HLB trees respond similarly to environmental changes between seasons. Statistically, the
percentage of small fruit has not changed for either variety over the 4 years of the study. This
means that although the percentage of small fruit does fluctuate from year-to-year, we cannot say
that it is increasing or decreasing at this time. In other words, the HLB affected trees being
observed in this study, on Maury Boyd’s nutritional program, are being maintained and are not
declining.
CREF Field Day, March 7, 2012 Page 20
These data provide support for the belief that HLB affected trees can in fact be maintained and
continue to produce an economically viable crop to sustain our industry until a longterm solution
to HLB is discovered.
Hamlin: Average yield per tree and the average percentage of fruit per tree less
than 2.4 inches in diameter for four harvest seasons.
Avg yield per tree (lbs) % of fruit < 2.4” diameter
Year Healthy HLB Healthy HLB
2008/09 478.4 300.8 1.0 12.6
2009/10 338.3 134.5 6.5 15.7
2010/11 356.4 193.9 5.4 19.8
2011/12 537.5 230.1 1.5 5.9
Valencia: Average yield per tree and the average percentage of fruit per tree
less than 2.4 inches in diameter for three harvest seasons.
Avg yield per tree (lbs) % of fruit < 2.4” diameter
Year Healthy HLB Healthy HLB
2008/09 309.6 145.4 1.0 7.8
2009/10 157.8 144.7 2.5 5.7
2010/11 274.5 180.6 0.4 3.9
CREF Field Day, March 7, 2012 Page 21
Wildlife seen in the CREF citrus grove (Photos by Nolan Rayburn)
Because the CREF grove is surrounded on most sides by the Florida Fish and Wildlife
Conservation Commission’s property, there is an abundance of wildlife in the grove. The deer
are a nuisance because of their browsing of young reset trees but the most troublesome animals
so far have been the feral pigs. On recommendation by the FFWCC, an electrified fence was
constructed around the grove to exclude the hogs, but is understandably having no useful effect.
As the photos show (Fig. 7), the hogs are intelligent animals and will find a way through or
under the fence.
Figure 13. Photographic record of wildlife seen in the CREF grove (photos by Nolan Rayburn)
CREF Field Day, March 7, 2012 Page 22
Damage to the grove from freezing in 2010/11
Figure 8. Fruit and canopy damage from freezes during winter of 2010/11 in the CREF grove.
Figure 14. Freeze damage seen in the grove during February 2011.
CREF Field Day, March 7, 2012 Page 23
Figure 15. Recent photos (December 2011) taken in the CREF grove before the’Hamlin’ harvest.
CREF Field Day, March 7, 2012 Page 24
Acknowledgements*
The CREC and the CREF would like to acknowledge all the people and organizations who
support(ed) the CREF grove and the research at Lake Placid:
Research and support staff of the CREC, especially Kevin Hostler, Troy Gainey, Nolan
Rayburn, Lioubov Polonik, Cynthia Basnaw, Laura Waldo, Ian Jackson, Angelique
Hoyte, and the Grove Management Department
Lykes Bros. – in-kind support during grove rehabilitation
G.P. Solutions – donation of foliar fertilizer products for 2010-2011 ("TKO" Phosphite
0-29-26, Companion Biological Fungicide, Nitro-30 SRN, Microtech AG, Recover-RX
3-18-18, and SAR Activator SA)
USDA/SCRI block grant – funds for experiments conducted from 2010-2012
FDACS BMP research grant – funds for BMP research conducted from 2005-2007
Scotts (now Everris) – donation of Citriblen CRF for BMP research experiment
*Where trade names appear, no discrimination is intended, and no endorsement by the
University of Florida is implied.