(classical) genetics
DESCRIPTION
(classical) genetics. causality. DNA. phenotype. ‘reverse’ genetics. T. C. The ‘gapped duplex’ method of in vitro mutagenesis. Gapped duplex method with phenotypic selection. Target : replace Trp by Phe Screening aid : Mnl I cleavage Mismatch primer : …GCCCT G GGCT TC GGTGGCA…. - PowerPoint PPT PresentationTRANSCRIPT
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(classical) genetics
causalityphenotypeDNA
‘reverse’ genetics
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TC
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The ‘gapped duplex’ method of in vitro mutagenesis
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Gapped duplex method with phenotypic selection
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Creating or removing a restriction recognition site as an aid in screening for site-specific mutants
Target : replace Trp by Phe
Screening aid : MnlI cleavage
Mismatch primer : …GCCCTGGGCTTCGGTGGCA…
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Codewoord tabel voor ‘reverse translation’
Leu = 1T2 Arg = 3G4 Ser = 567
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