clinical chemistry 1

8/16/2019 Clinical Chemistry 1

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Part II: Course Syllabus

Table of Contents

Item Page

Instructor Information 2-1

Course Information2-2

Course Objectives2-4

Learning Resources2-22

Course Requirements & Grading 2-28

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Instructor Information

DR. ESSAM HUSAIN JIFFRI (Course Co-ordinator) Tee!"one# $%&e# '0000 E*. 2+,

Moie# 00'+/E-ai# e1iri34aau.edu.sa

$%&e 5o&ation# st 6oor7 Roo No. 28 $%&e Hour# Sat# 0#00 9 2#00 :M ;ed#0#00 9 2#00 :M

R! "#$% "O'(#- Tee!"one# $%&e# '0000 E*. 220,/ 82020/

Moie# 00'02, - E-ai# "4"o1a34aau.edu.sa - $%&e 5o&ation# 2nd 6oor7 Roo No. 282 - $%&e Hour# Sun /#00 9 #00 AM

Tue /#00 9 #00 AM

DR. ADEE5


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CO'R)% *#$%# Cini&a C"eistr@-

CO'R)% *'$+%R# M5T +0

CO'R)% $%%,I*G ,I$%)

Lecture hours: + "ours8ee4 Bor ee4s (Tota 2 "ours)

Practical hours: 2 "ours8ee4 Bor ee4s (Tota 2>"ours)

Tutorial hours: "our8ee4 Bor ee4s (Tota "ours)

LECTUREThree/Week

PRCTICLST!o/Week

TUT"RILS"ne/Week

#LE

Sat 0>#00-0/#00a.

Sat 02#0090#00 !. (


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CO'R)% /%+)I,% #R%))

CO'R)% .R%R%0'I)I,%) #* R%0'IR%$%*,)

A su&&essBu &o!etion oB io&"eistr@ (M5T 20) it" a na

&uuatie rade oB aoe '0G is t"e !re-reuisite Bor Cini&a

C"eistr@- (M5T +0).

%*,R L%%L )ILL)

T"e student s"oud dis!a@ asi& readin7 ritin7 and at"eati&as4is

%)CRI.,IO* O3 ,"% CO'R)%

T"is &ourse i e a detaied stud@ oB t"e &"ei&a and

instruenta ana@ses oB "uan iooi&a ateria oB &ini&a

sini&an&e. T"e student i e introdu&ed to &oon@ used

&ini&a &"eistr@ te&"niues. T"is &ourse dis&usses t"e &ini&a

as!e&ts oB 5a Mat"7 Sour&es oB errors7 &aro"@drates7 Aino-

a&ido!at"es7 !roteins7 itains7 Tra&e Eeents7 en@es7

ee&tro@tes. T"is &ourse i interate t"e Booin to!i&s into t"e

aorator@ and e&ture sessions# aorator@ at"eati&s7 uait@

assuran&e7 s!e&ien &oe&tion and !ro&essin7 sour&es oB

iooi&a ariation7 and eauation te&"niues.

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Course "b&ecti'es

G%*%R#L O+(%C,I%)

U!on &o!etion oB t"is &ourse t"e student i e ae to#

A&"iee t"e o1e&ties desinated @ a uet under ea&" e&ture7

rou! oB e&tures or !ra&ti&a sessions.

2 -


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O+(%C,I%) O3 L%C,'R% ,O.IC)

T"e &ourse &onsists oB e&tures7 !ra&ti&a &asses7 deonstrationsand tutorias. T"e Booin is a istin oB e&ture to!i&s and nuer

oB e&tures Bor ea&" to!i

L%C,'R% ,O.IC)*'$+%R O3L%C,'R%)

- Course S&o!e +

2- asi& K 5a Mat" +

+- Sour&e K T@!es oB Errors KLC +

- Caro"@drates '

- :roteins '

'- Tra&e Eeents +

,- itains +

>- En@oo@ '

/- Ee&tro@tes K Ca7 M7 : /

,O,#L 42

2 -


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() *SIC SC"PE +L* #T,

-Si. lectures

"b&ecti'es:

To a4e sure t"at t"e student is ae to#

. reaie7 understand and dea it" nueri&a aues in t"e

&ini&a a in t"e est !ossie a@ to re6e&t a&&ura&@ and

!re&ision oB t"e ien aue (sini&ant ures7 de&ias7

s&ienti& notation7 roundin o nuers)

2. re&onie7 understand and inter!ret SI units as a!!ies to

ass7 oue7 ent"s7 area K &on&entrations

+. &arr@ out &onersions oB sine units it"in t"e SI s@ste

. &arr@ out &onersions oB &oined units it"in t"e SI s@ste

. re&onie7 understand and inter!ret &oon@ used

&onentiona units su&" as 8d5 n857 !85

'. 4noin t"e Borua ei"t oB sustan&es7 or a!!ro!riate

&onersion Ba&tor7 e ae to &onert Bro &onentiona to SI

units.

,. 4noin t"e Borua ei"t oB sustan&es7 or a!!ro!riate

&onersion Ba&tor7 e ae to &onert Bro SI units to

&onentiona units>. &a&uate t"e &on&entration oB soutions in arious ters su&"

as 85 o857 85.

/. &a&uate oarit@ and norait@ oB soutions.

0. e*!ress t"e aount oB sustan&es in ters oB res!e&tie

!er&entaes (i.e G 8 G 87 G 8).

. or4 out sine7 uti!e7 seria and !arae diutions.

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2. &a&uate t"e &on&entration oB ana@tes in t"e neat

sa!e "i&" as diuted @ a s!e&i& diution Ba&tor.

+. &a&uate t"e na &on&entration oB soution "i&" as

diuted @ a s!e&i& diution Ba&tor.

. or4 out "o to !re!are arious soutions Bro sto&4

soutions

. or4 out "o to !re!are arious reaents Bro arious

&o!ounds and sustan&es

'. 4noin t"e s!e&i& rait@ and G a&ids or4 out "o to

!re!are ea4er &on&entrations oB a&ids Bro &on&entrated

sto&4 soutions

,. &a&uates t"e ean7 SD K &

>. &a&uates G deiation

0) S"URCES + T1PES "$ ERR"RS

-Si. lectures

"b&ecti'es

T"e student ust e ae to

- des&rie t"e dis&i!ine oB &ini&a &"eistr@.

2- e*!ain t"e i!ortan&e oB &ini&a &"eistr@ in a edi&ine.

+- des&rie t"e Bun&tion oB t"e &ini&a &"eistr@ a.

- des&rie t"e t@!e oB resuts otained in t"e &ini&a as.

- des&rie t"e &"ara&teristi&s oB uantitatie resuts.

'- des&rie and !erBor &airation oB !i!ettes used in t"e a @

raietri& !ro&edures.

,- des&ries t"e asi& uait@ &ontro and uait@ assuran&e

reuireents Bor t"e a.

>- des&rie reiaiit@7 a&&ura&@ and !re&ision oB resuts.

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/- des&rie sensitiit@ and s!e&i&it@ oB !ro&edures.

0- des&rie t"e t@!es oB ana@ti&a errors in t"e a.

- des&rie t"e ee&ts oB t"ese errors on t"e resut oB tests.

2- des&rie t"e t"ree staes oB uait@ assuran&e in t"e a.

+- e*!ain t"e !reana@ti&a Ba&tors t"at a@ ead to errors in

t"e ana@sis.

- e*!ain t"e ana@ti&a Ba&tors t"at a@ ead to errors in

t"e ana@sis.

- e*!ain t"e !ostana@ti&a Ba&tors t"at a@ ead to errors

in t"e re!orted resuts.

'- des&rie iits oB a&&ura&@ oB t"e re!orted resut in ters

oB

a. re!orted aues

. de&ia !a&es

&. !ro&edure iitations

d. instruent iitations

e. &ini&a sini&an&e

B. assa@ rane

. &ini&a rane

". e*tent oB inearit@

i. reaents

1. &airators

4. te!erature

. "uidit@

. iration

n. air &urrent

o. interBeren&e

!. &ross-rea&tiit@

. sensitiit@ iits

r. s!e&i&it@

2 - >


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s. reaent an4

,- des&rie "o to s!ot errors in t"e a.

>- des&rie "o to eiinate or iniie t"e sie and

Breuen&@ oB errors.

/- outine a@s to i!roe a&&ura&@7 !re&ision oB resuts.

20- outine !ossie ste!s to i!roe t"e sensitiit@ and

s!e&i&it@ oB et"ods in t"e a.

2- re&onie t"e need Bor &ontinuous deeo!ent and

odi&ations oB e*istin s@stes in t"e a and outside t"e

a.

22- des&rie t"e a1or !re&autions t"at ust e osered in

t"e a to ensure t"e reiaiit@ oB t"e re!orted resuts.

2) CR*",13RTE #ET*"LIS#

-Si. lectures

"b&ecti'es

U!on &o!etion oB t"is &"a!ter7 t"e student i e ae to#

1- IdentiB@ t"e !riar@ iooi&a Bun&tion oB &aro"@drate in

"uans.

2- Des&rie7 in enera ters7 t"e etaois oB

&aro"@drates in "uans.

3- Co!are and &ontrast t"e Bour t@!es oB utiiation oB

u&ose# @&oenesis7 u&oneoenesis7 @&o@sis7

@&oeno@sis.

2 - /


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4- $utine t"e Boration oB ot"er &onstituents7 su&" as

@&oen7 aa&tose7 Bru&tose7 !@rui& a&id7 a&ti& a&id7 Bro

u&ose.

5- Dis&uss t"e i!ortan&e oB t"e anaeroi& and aeroi&

!at"a@s and t"e !entose !"os!"ate s"unt in t"e

etaois oB u&ose.

6- IdentiB@ and e*!ain t"e roe oB t"e Booin "orones in

u&ose etaois

a. Insuin

.


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a. ood u&ose

.


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>- For ea&" test isted eo des&rie t"e rationae7

&ini&a useBuness7 s!e&ien reuireents7 !atient

!re!aration7 !ro&edure7 !rin&i!e(s)7 easureent

used to

&a&uate a&tiit@7 iitations7 and reBeren&e rane(s)#

a -


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4) #I5" CI3"PT,IES

-Three lectures

"b&ecti'es:

Students are ien t"e Bundaenta &on&e!ts oB etaoi&

disorders and t"eir !at"a@s. A&&uuation and suseuent

oer6o oB !re&ursors due to t"e de&ien&@ or ina&tiit@ oB a &ertain

etaoite (aino-a&id &ontainin !rotein or en@e) are

&onsidered t"e asis oB t"ese disorders. Dete&tion oB t"ese

etaoites in ood and t"eir oer6o into t"e urine is &ini&a@

sini&ant.

Dis&uss t"e etaoi& disorders are rare "oeer soe oB t"e are

ore &oon t"an ot"ers and t"ese in&ude#

• :"en@4etonuria# In"erited autosoa re&essie disorder "ere

ear@ dete&tion oB a&&uuatin etaoite is &ru&ia Bor etter

!ronosis

• A4a!tonuria

• T@rosinuria# T@!eI and T@!e II

• Ma!e S@ru! Urine Disease

• Hoo&@stineuria

•C@stinuria

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T"e to!i&s &oered in&ude arious et"ods oB dete&tion7 s&reenin

tests and reBeren&e et"ods. Aso &riteria Bor ood and urine

sa!e &oe&tion is dis&ussed

6) PR"TEI5S

-Three lectures

"b&ecti'es:

T"e students are reuired to 4no t"e &ini&a sini&an&e oB

!roteins and to dierentiate eteen arious Bra&tions oB !roteins

de!endin u!on t"eir Bun&tions. As aorator@ te&"nooists t"e

students ust 4no dierent et"ods oB dete&tion and t"eir

under@in !rin&i!es.

T"e to!i&s &oered in&ude#

• Stru&tures oB !roteins7 a!"oteri& !ro!ert@7 &on&e!ts oB

&ataois and anaois

•


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c. A!"a- ouins

d. A!"a-2 ouins

e. eta ouins

f.


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Students are ade aare oB t"e i!ortan&e oB itains as essentia

&o!onent oB dai@ nutrition. I!ortant itains are &oered t"at

in&udes# itain-A7 C7 D7 and &o!e*. T"eir triia naes7

reBeren&e ranes7 dietar@ sour&e and Bun&tions are &oered.

To!i&s in&udin &ini&a s@!tos due to t"eir de&ien&@ or

oerdose are aso e*!ained Booed @ arious et"ods oB

dete&tion "i&" in&ude#

• In-io and in-itro ioassa@s

• Mi&roiooi&a assa@s

• itain oadin tests

• Fouroetri& and Coorietri& ana@sis

• H:5C

• RIA K E5ISA

• A!eroetr@

• Er@t"ro&@te Braiit@ test

• :rot"roin and !artia t"roin test

• A&tiit@ &oe%&ient test

To understand t"e adantaes and disadantaes oB ea&" oB t"e

aoe et"od is des&ries and t"e students are ade e aare oB

t"e reBeren&e et"ods and s&reenin et"ods used.

9) TRCE ELE#E5TS

-Three lectures

"b&ecti'es:

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To understand "at t"ese tra&e eeents areQ And to a!!re&iate

t"eir !resen&e in t"e "uan od@.

To understand t"e nutritiona aue and Bun&tiona i!ortan&e oB

essentia7 oderate@ essentia and non-essentia tra&e eeents.

To understand t"e nutritiona aue and Bun&tiona i!ortan&e oB

utra-tra&eeents

Tra&e eeents &oered in t"is &"a!ter in&ude#

• Iron# nora ranes7 !roteins &arriers7 &ini&a sini&an&e7

et"ods oB dete&tion7 transBerrin and Berritin7 Iron saturation

and indin &a!a&it@. Iron !i&ture in arious t@!es oB aneias

• Co!!er# &ini&a sini&an&e7 Bun&tions and trans!ortin

en@e (&eruo!asin)7 et"od oB dete&tion7 t@!es oB sa!e

and interBerin sustan&es

• in Bun&tion7 &ini&a sini&an&e7 et"od oB dete&tion7 t@!es

oB sa!e and interBerin sustan&es

Utra-tra&e eeents &oered in t"is &"a!ter in&ude#

• Seeniu# Fun&tion in asso&iation it" itain-E and

utat"ione !ero*idase7 &ini&a sini&an&e7 et"ods oB

dete&tion7 t@!es oB sa!e and nora rane

•

Mananese# Bun&tion and &ini&a de&ien&@7 t@!e oB sa!ereuired

• Coat# asor!tion in reation to itain 2 and intrinsi&

Ba&tor.

) E5;1#"L"


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"b&ecti'es:

U!on &o!etion oB t"is &"a!ter7 t"e student i e ae to#

- E*!ain t"e dierent Ba&tors ae&tin seru en@e#

• Rate oB entr@ oB en@es into ood

• En@e in"iitors

• Cearan&e oB en@e

2- E*!ain s!e&i&it@ oB seru en@e easureents#

• Test resuts and &ini&a Beatures

• Test !attern re&onition

• Isoen@es

+-


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. - Dis&uss t"e dierent et"ods Bor t"e deterination in seru

and urine.

/- Assess s!e&ien interit@ Bor en@e ana@sis ased on

sa!es

a. &oe&tion

2 - /


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. !re&essin7 and

&. storae &onditions

=) ELECTR"L1TES -5a> ?> Cl> + Ca> #g> Pi

- 5ine lectures

"b&ecti'es:

T"e student ust e ae to

- des&rie t"e !"@siooi&a roes oB Na7 7 C7 Ca7 M K :i

2- des&rie t"e a1or Ba&tors t"at in6uen&e t"e &on&entration oB

t"ese sustan&e in t"e od@ and ood.

+- des&rie t"e &ini&a sini&an&e and &orreations in t"e

easureents oB Na7 7 C7 Ca7 M K :i.

- des&rie t"e s!e&ien reuireents Bor t"e deterination oB

Na7 7 C7 Ca7 M K :i.

- des&rie t"e !ossie sour&es oB errors in t"e deterination oB

Na7 7 C7 Ca7 M K :i.

'- des&rie t"e ee&ts oB su&" errors on t"e resuts otained Bor

ea&" oB t"e ana@tes (i.e Base in&rease or Base de&rease).

,- des&rie a1or interBerin Ba&tors t"at a@ ae&t t"e resuts.

>- des&rie "o to eiinate interBeren&es.

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/- des&rie t"e a@s to iniie or eiinate t"e arious sour&es

oB errors.

0-des&rie t"e reBeren&e et"ods Bor deterination oB Na7 7 C7

Ca7

M K :i.

-des&rie t"e ost &oon@ used et"ods Bor t"e

deterination oB

Na7 7 C7 Ca7 M K :i "i&" are used in t"e &ini&a a.

2-e*!ain "o to use a resuts to dierentiate eteen

arious t@!es

oB "@!onatreia.

+-e*!ain t"e ter !ani& aues.

-ist t"e !ani& aues Bor Na K .

-e*!ain t"e reations"i! eteen a&id ase disturan&es and

Na7 7

C7 Ca K M.

'-rite t"e Ma&5ean-Hastins Forua Bor t"e &a&uation oB

ionied

Ca (Ca2).

,-&a&uate t"e Ca2 &on&entration usin t"e Ma&5ean-Hastins

Forua.

>-ist t"e &onditions Bor t"e use oB t"e Ma&5ean-Hastins

Forua

Bor t"e &a&uation oB Ca2.

/-des&rie t"e &oon et"ods used to easure Ca2 .

20-rite t"e Borua used to &a&uate t"e anion a! (A


23/32

2+-des&rie t"e &ini&a sini&an&e Bor t"e &a&uation oB A< in

ood

a. in&reased A<

. de&reased A<

&. a!!arent@ nora A<

2-des&rie t"e &ini&a sini&an&e Bor t"e &a&uation oB A< in

urine.

2-e*!ain t"e ee&t oB &oined errors on t"e &onsiderations

oB t"e

A< aues in &ini&a situations oB etaoi& a&idosis.

2'-e*!ain "o A< is used in t"e &ini&a a.

LER5I5< RES"URCES

,%, +OO) & R%#I*G $#,%RI#L)#

Assigned Textbook :

Cinica C!e"ist#$% inci'es% oced(#es% and Co##eations% 3#d )d.

*$ +ic!ae *is!o'% )d,a#d odd$% and anet /(ben

.*. 0i''incott % &!iade'!ia% 25o# atest edition.

eco""ended efe#ences :

1- Cinica /iagnosis and +anage"ent% b$ o!n *e#na#d en#$%ed.

2 - 22


24/32

.*. a(dne#s% &!iade'!ia.atest edition.

2- (nda"entas of Cinica C!e"ist#$ Tiet% atest edition.% .*. a(nde#s a'an

&esce. atest edition.

3- Cinica C!e"ist# T!eo#$ Ana$sis Co##eation% atest ed. C.. +osb$

Cinica C!e"ist#$ in /iagnosis and T#eat"ent% ;ia &anne%

atest edition.

/%+)I,% #R%))%)

!tt':


25/32

Fa&ut@ oB a!!ied edi&a s&ien&es7Feae se&tion

2 - 2


26/32

L#+OR#,OR "O'R)

PRCTICLST!o/Week

#LE

Sat 02#00 9 0#00 (


27/32

:RACTICA5 5A$RAT$R= $JECTIES

Hain attended a oB t"e !ra&ti&a a sessions7 istened to and

understood t"e e*!anation oB t"e !ro&edure done7 at&"ed a

deonstration @ t"e instru&tor (or t"e a te&"nooist in &"are)7

and read a t"e aterias assined7 t"e student ust e ae to

!erBor a oB t"e e*!erients (tests7 !ro&edures7 et&.) e*!ained

and tau"t. T"e student ust e ae to e*"iit a satisBa&tor@

&o!eten&e ee @ a&"iein no ess t"an '0G oB an@ ien

!ra&ti&a e*a !erBored it"in a suita@ assined tie.

Genera6 Laborator7 Objectives

. Des&rie t"e t@!es7 !re!aration7 use and storae oB &"ei&as

used in

a &"eistr@ aorator@.

2. Dis&uss t"e &o!osition7 use7 and iitations oB aorator@

assare7 !asti& are and tuin.

+. IdentiB@ t"e arious t@!es oB ouetri& eui!ent in

&oon usae in t"e &"eistr@ aorator@.

. Des&rie !roto&os Bor t"e &airation oB ouetri&

eui!ent7 its use and aintenan&e.

. E*!ain t"e t"eories oB o!eration oB &entriBues and

aan&es.

'. Dis&uss &airation aintenan&e reuireents7 and

enironenta Ba&tors inoed in t"e use oB &entriBues

and aan&es.

,. Des&rie t"e !rin&i!es oB !ro&edures used to !re!are sa!es

and soutions Bor ana@sis.

>. Dis&uss t"e &o!onents7 !re!aration7 sour&es oB error and

e*!ressions oB &on&entration oB soutions.

2 - 2'


28/32

/. Dene t"e &o!onents oB uer s@stes and its roe in t"e

aintenan&e oB !H.

0. Des&rie t"e t@!es oB saBet@ "aards Bound in t"e

&"eistr@ aorator@.

. IdentiB@ s!e&i& !re&autions and saBet@ eui!ent

used to iniie or !reent aorator@ "aards.

5aorator@ To!i&s#

. Cin. C"e. 5a Fun&tion

2. Sour&es oB Errors E*er&ise

+. 5a Mat" E*er&ise

.


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+. ;"en ien asor!tiit@ &onstant and &on&entration7 &a&uate

t"e asoran&e.

. Assess auin usin t"e C< d@e et"od it" resuts it"in G

oB easured aue.

. Co!are and &ontrast one-!oint &airation it" t"at oB a

standard &ure.

'. :erBor ee&tro!"oresis and identiB@ a nora !attern.

,. ;"en ien a ist oB asoran&e aues &a&uate t"e &"ane in

asoran&e.

>. ;"en ien asoran&e aues7 ent" oB i"t !at" and oar

asor!tiit@ oB a sustan&e7 &a&uate t"e U aue.

/. Co!are and &ontrast end!oint and 4ineti& en@e assa@s.

0. Ad1ust t"e as!irati'n rate oB t"e atoier on t"e 6ae

!"otoeter.

. Set t"e standards oB t"e 6ae !"otoeter.

2. Ana@e &ontros and !atient sa!es on t"e 6ae !"otoeter

+. :erBor t"e &"oride deterination and &a&uate t"e

&on&entration oB

t"e &ontro and !atient sa!es usin t"e &"oride eter.

. :erBor it"in G and it" a&&e!tae te&"niue t"e arious

u&ose

test et"ods.

. :erBor t"e Booin assa@s it" a&&e!tae te&"niue7

!re&ision7

and a&&ura&@#

'. A&&ession s!e&iens and eauate t"e as to t"eir suitaiit@ Bor

t"e deterination reuested.

,. :re!are aiuots oB s!e&iens and distriute t"e Bor ana@sis.

>. Maintain re&ord 4ee!in Bor s!e&iens.

/. :re!are reaents a&&ordin to !res&ried dire&tions.

2 - 2>


30/32

20. Deonstrate e%&ient o!eration oB aorator@ instruents to

a&&e!tae ees Bor t"e instru&tor.

2. :erBor and inter!ret ana@ses on !atient s!e&iens and

&ontros

a&&ordin to !roto&o.

22. Eauate test resuts it" resuts a&&e!tae to t"e instru&tor.

Course Re@uirements +


31/32

to as7 t"e instru&tor a@7 ut is not oiated to e ait"draa.

2 - +0


32/32

%#L'#,IO* ),R#,%GI%)5GR#I*G

Note# IB a student ust e asent on t"e da@ oB a test7 "e8s"eust notiB@ t"e instru&tor !rior to test tie in order to eaoed to ta4e a a4e-u! test. A rade oB ero (0) i eassined iB t"e instru&tor is not notied. IB t"e student e*&eeds

t"e a*iu asen&es oB 0G7 t"is i resut in "is8"er eindro!!ed Bro t"e &ourse and Bro t"e M5T !rora.

GR#I*G )C#L%

T"e Booin rade s&ae a!!ies t"rou"out t"e &ourse#

E*&eent O /0.0G - 00.0G

er@ ood O >0.0G - >/.0G

clinical chemistry 1

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