clinical group n biology of hiv infection n by duangrat inthorn n mechanism of reverse transcriptase...
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Clinical GroupClinical Group
Biology of HIV infection Biology of HIV infection by Duangrat Inthornby Duangrat Inthorn Mechanism of ReverseMechanism of Reverse Transcriptase InhiTranscriptase Inhi
bitors and Protease Inhibitorsbitors and Protease Inhibitors by Tawitch Suriyoby Tawitch Suriyo Animal Models for HIV Animal Models for HIV by Pornpat Intarasunanontby Pornpat Intarasunanont
Clinical Group (continue)Clinical Group (continue)
Clinical Trails- CrixivanClinical Trails- Crixivan by Somjed Sahasitiwatby Somjed Sahasitiwat Clinical Trails-Drug Interactions and SiClinical Trails-Drug Interactions and Si
de Effectsde Effects by Peerakarn Banjerdkijby Peerakarn Banjerdkij Combination Therapy and Resistance Combination Therapy and Resistance by Ormrat Kampeerawipakornby Ormrat Kampeerawipakorn
Clinical Group (continue)Clinical Group (continue)
Targeting of the CD4 Binding Site Targeting of the CD4 Binding Site by Tan Swee Huangby Tan Swee Huang
Gene TherapyGene Therapy by Pairoaj Vonghathaipaisarnby Pairoaj Vonghathaipaisarn DNA vaccinesDNA vaccines by Zhang Jianjunby Zhang Jianjun
The Biology of HIV InfectionThe Biology of HIV Infection
Asst. Prof. Dr. Duangrat InthornAsst. Prof. Dr. Duangrat Inthorn
Dept. of Environmental Health SDept. of Environmental Health Science, Faculty of Public Health, cience, Faculty of Public Health,
Mahidol UniversityMahidol University
ScopeScope
Biology of HIV , structure of HIV, Biology of HIV , structure of HIV, genome of HIV, life cycle of HIVgenome of HIV, life cycle of HIV
HIV -1 and related RetrovirusHIV -1 and related Retrovirus How it might be stopped (Drug devHow it might be stopped (Drug dev
elopment)elopment)
Introduction
The virus that cause AIDS (Acquired Immunodeficiency Syndrome, AIDS) is HIV (Human Immunodeficiency Virus, HIV)Homosexual have Pneumocyatis carnii together with herpes simplex, cytomegalovirusand CandidaKaposi’s sarcoma- KS is a few case in healthy person
Structure of HIV
-Outer lipid bilayer coat studded with surface (SU, gp 120)-transmembrane (TM,gp41) glycoprotein complexs-beneath the lipid bilayer are matrix proteins (MA)-the virion consist of internal capsular (CA) andnuclear capsid (NC) proteins which surround the single stranded RNA genome.
HIV virion
- show the components and their genetic source-the matrix (MA), internal capsular (CA), and nuclearcapsid (NC) proteins -produce by the gag region-contain gene products of the pol region- RT, integrase and protease.
The Genome of HIVThe Genome of HIV
HIV genome is 9749 nucleotides about HIV genome is 9749 nucleotides about the same size as any other retrovirus, fthe same size as any other retrovirus, for example Rous sarcoma virus (RSV)or example Rous sarcoma virus (RSV)
But genome of HIV is more complex tBut genome of HIV is more complex than RSV since it has extra open readinhan RSV since it has extra open reading frames that clearly code for small prog frames that clearly code for small proteins.teins.
The Genome of HIVThe Genome of HIV
The HIV genome has The HIV genome has ninenine open reading open reading frames but 15 proteins are made in allframes but 15 proteins are made in all
The The GAG/POLGAG/POL polyprotein and the polyprotein and the ENENVV polyprotein are cleaved into several p polyprotein are cleaved into several proteins:roteins:
GAGGAG is cleaved to: MA (matrix), CA(c is cleaved to: MA (matrix), CA(capsid), NC (nucleocapsid), p6apsid), NC (nucleocapsid), p6
POLPOL is cleaved to: PR(protease), RT(reverse is cleaved to: PR(protease), RT(reverse transcriptase), IN (integrase)transcriptase), IN (integrase)
ENVENV is cleaved to : SU (gp120) and (gp41) is cleaved to : SU (gp120) and (gp41) Of other proteins, three are incorporated into Of other proteins, three are incorporated into
the virus the virus (Vif, Vpr and Nef)(Vif, Vpr and Nef) while three are while three are not found in the mature virus: not found in the mature virus: Tat and rev arTat and rev are regulatory proteinse regulatory proteins and and Vpu indirectly assiVpu indirectly assists in assemblysts in assembly
TAT : Trans-Activator of Transcription
REV: Regulator of Virion protein expression
NEF : Negative Regulatory Factor
VIF : Virion Infectivity FactorVPU : Viral Protein UVPR : Viral Protein R
These genes encode small proteins. Theyoverlap with the structural genes (especially ENV) but are different reading frames.