Background/Objective: Matrix metalloproteases (MMPs)have been associated with aggressiveness in some cancers. Weinvestigated possible associations of SNPs in MMPs genes andtumor-stage/nodal-metastasis in head and neck squamous cellcarcinoma (HNSCC).

Study design: Leukocyte DNA was extracted of HNSCCpatients and SNPs with higher chances of functional effects wereevaluated using TaqMan assays in an ABI7500. A total of 318patients samples were investigated with four genes/SNPs:MMP14, rs1042703 – SER8PRO – nsSNP; MMP16, rs2616490– ILE415VAL –nsSNP; MMP25 (MMPL1), rs10431961 – sSNP;and TIMP-3, rs1065314 – 3’ UTR. SPSS 17.0 was used forstatistical analysis.

Results: Advanced tumors (T3-T4) were present in 156patients (49%) and 158 patients (49.6%) presented nodal metas-tasis. Allelic/genotypic frequencies showed no correlation toclinical aspects of HNSCC.

Conclusion: Besides the importance of MMPs for the pro-gression of HNSCC, the SNPs studied here showed no statisticcorrelation with more or less aggressive HNSCC.

CLINICAL INTRAORAL PROFILE OF SECONDARYSYPHILIS IN ORAL MEDICINE SERVICE AT ARARA-QUARA SCHOOL OF DENTISTRY Ohata G1,2,3,4 - 1ARA-RAQUARA SCHOOL OF DENTISTRY - UNESP - DIAGNO-SIS AND SURGERY, 2ELAINE MARIA SGAVIOLIMASSUCATO - DIAGNOSIS AND SURGERY/ARARA-QUARA SCHOOL OF DENTISTRY, 3MIRIAN APARECIDAONOFRE - DIAGNOSIS AND SURGERY/ARARAQUARASCHOOL OF DENTISTRY, 4CLÁUDIA MARIA NAVARRO -DIAGNOSIS AND SURGERY/ARARAQUARA SCHOOL OFDENTISTRY

The objective of this study was to assess cases of secondarysyphilis to establish a clinical intraoral profile. This was a retro-spective study based on clinical files of patients attended at theOral Medicine Service of the Araraquara Dental School, between1981 and 2012. The sample was composed by 7 patients withintraoral secondary syphilis serologically confirmed, being 4cases diagnosed in 2012. The patients were predominantly men(6), with the mean age of 32 years old. The main complaints are:oral lesions (5) and burning mouth (3) associated to skin rash twomonths before the exam (5) and cervical lymphadenopathy (2).The main anatomical sites affected were: labial mucosa (5) andtongue (5). The Intraoral Secondary Syphilis occurs mainly inwhite men, mean age 32 years, in the era of highly activeantiretroviral therapy.

A CASE OF CUTANEOUS HORN ASSOCIATED WITHINJURY OCCURRING ON THE VERMILION BORDEROF THE LOWER LIP Oliveira GMR1, Thomé EC2, RamosTR1, Pereira HSC1, Resende RFB2, Meirelle MS2 - 1UERJ -UNIVERSIDADE ESTADUAL DO RIO DE JANEIRO,2DENTAL OFFICE

Cutaneous horn is a morphological description of a highlyconic and circumscribed keratotic lesion found in both sexes andoccur most commonly in light-skinned patients over 50 years.The surface can be white or yellowish and that can hide benign,premalignant or malignant lesions. This impressive retention ofkeratin is unknown. It’s most frequently caused by sun-exposedskin. Histopathologically, shows a compact, diffuse hyperkera-tosis and parakeratosis. The aim of this study is to report the case

of a 51 years old Caucasian man presenting an exophytic lesion,rigid palpation and sessile base and about 3 cm in diameter after2 years of evolution. The patient underwent excisional biopsy ofthe lesion under local anesthesia, and its histopathology compat-ible with the presence of actinic keratosis and cutaneous hornassociated with injury.

LOSS OF HETEROZYGOSITY (LOH) IN TUMOUR SUP-PRESSOR GENES IN ORAL GRANULAR CELL TUMOURSilva TF1, Correa GTB1, Campos K1, Amaral FM2, GomesCC3, Gomez RS4 - 1UNIVERSIDADE FEDERAL DE MINASGERAIS - DEPARTAMENT OF MOLECULAR MEDICINE,2UNIVERSIDADE FEDERAL DE MINAS GERAIS - DEPAR-TAMENT OF BIOLOGY, 3UNIVERSIDADE FEDERAL DEMINAS GERAIS - DEPARTAMENT OF PATHOLOGY,4UNIVERSIDADE FEDERAL DE MINAS GERAIS - DEPAR-TAMENT OF ORAL SURGERY AND PATHOLOGY -SCHOOL OF DENTISTRY

Objective: The objective of this study was to investigate lossof heterozygosity (LOH) in regions of tumor suppressor genes inoral granular cell tumor (GCT).

Study design: LOH was assessed in eight samples of oralGCT using polymorphic microsatellite markers at the chromo-some regions 3p, 9p, 11p, 11q and 17p (markers D3S1029,D3S1293, D9S157, D9S162, D9S171, D11S1369, AFM238WF2and P53) related to tumor suppressor genes.

Results: Five samples showed LOH in three markers atchromosomes 9p and 17p (markers TP53, AFM238WF2 andD9S162) with fraction of allelic loss of 42.8% for each of thesemarkers. Moreover, no allelic loss was identified in any otherchromosome.

Conclusion: The results suggest that loss of heterozygosityat chromosomes 9p and 17p may be relevant to the pathogenesisof oral GCT.

Support: CAPES, CNPq, FAPEMIG

SOLITARY FIBROUS TUMOUR OF PALATE, A CASEREPORT Martínez-Martínez EA1, Meza-García G2, Cano-Valdés AM3, Domínguez-Malagón H3 - 1UNIVERSIDAD AU-TÓNOMA ”BENITO JUÁREZ” DE OAXACA - FACULTADDE ODONTOLOGIA, 2UNIVERSIDAD REGIONAL DELSURESTE/SERVICIOS DE SALUD DEL ESTADO DE OAX-ACA - ORAL PATHOLOGY, 3INSTITUTO NACIONAL DECANCEROLOGÍA DE MÉXICO - PATHOLOGY

Solitary fibrous tumour (SFT) is a spindle-cell mesenchymalneoplasm, has been reported in various locations but the oral cavityis a distinctly uncommon region. Case report: a female patient, 30years old with a painful tumour the lesion measured approximately4 cm; computed tomography showed a heterogeneous large mass inpalate extending to oral cavity preserving osseous tissue; an inci-sional biopsy was performed; histopathological examination dem-onstrated a circumscribed lesion composed mainly of hyalinizedfibrous connective tissue with intermittent paucicellular and hy-percellular areas and a hemangiopericytoma-like vasculaturewere noted. Immunohistochemically: were strongly positive forCD34, bcl-2 and vimentin but negative for S-100 protein, cal-ponin, CD-99. CD-21, CD-23, CD-35, Fascin and EMA; a diag-nosis of solitary fibrous tumor was made. The tumour was treatedby complete surgical excision; no recurrence was noted at a4-year follow-up.

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e116 Abstracts October 2012