clinical pathology: c-reactive protein

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CLINICAL PATHOLOGY: CLINICAL PATHOLOGY: C-Reactive Protein C-Reactive Protein GENERAL OBJECTIVE GENERAL OBJECTIVE : : After finishing lab activity of C-RP, After finishing lab activity of C-RP, the student will be able to describe the student will be able to describe C-RP in the DMS case problem C-RP in the DMS case problem SPECIFIC OBJECTIVE SPECIFIC OBJECTIVE : : At the end of lab activity of C-RP, At the end of lab activity of C-RP, the student will be able to the student will be able to interprete: interprete: - the normal and abnormal C-RP - the normal and abnormal C-RP

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CLINICAL PATHOLOGY: C-Reactive Protein. GENERAL OBJECTIVE : After finishing lab activity of C-RP, the student will be able to describe C-RP in the DMS case problem SPECIFIC OBJECTIVE : At the end of lab activity of C-RP, the student will be able to interprete: - the normal and abnormal C-RP. - PowerPoint PPT Presentation

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Page 1: CLINICAL PATHOLOGY: C-Reactive Protein

CLINICAL CLINICAL PATHOLOGY:PATHOLOGY:

C-Reactive ProteinC-Reactive ProteinGENERAL OBJECTIVEGENERAL OBJECTIVE::After finishing lab activity of C-RP, the After finishing lab activity of C-RP, the student will be able to describe C-RP in the student will be able to describe C-RP in the DMS case problemDMS case problem

SPECIFIC OBJECTIVESPECIFIC OBJECTIVE::At the end of lab activity of C-RP, the student At the end of lab activity of C-RP, the student will be able to interprete:will be able to interprete:- the normal and abnormal C-RP - the normal and abnormal C-RP

Page 2: CLINICAL PATHOLOGY: C-Reactive Protein

C-Reactive ProteinC-Reactive Protein

►C-RP is a serum constituent originally C-RP is a serum constituent originally define by its ability to precipitate define by its ability to precipitate Pneumococcus C polysaccharidePneumococcus C polysaccharide

►C-RP is an acute phase reactant:C-RP is an acute phase reactant:

A protein synthesized by the liver as a A protein synthesized by the liver as a non-specific response to the non-specific response to the inflammationinflammation

Page 3: CLINICAL PATHOLOGY: C-Reactive Protein

►C-RP concentration increased in the C-RP concentration increased in the serum of individu as a response to serum of individu as a response to varies inflammatory condition and varies inflammatory condition and tissue necrosistissue necrosis

►before specific antibody production before specific antibody production starts and the ESR is increased.starts and the ESR is increased.

►After successful therapy, CRP level After successful therapy, CRP level fall before the ESR decreasesfall before the ESR decreases

C-Reactive ProteinC-Reactive Protein

Page 4: CLINICAL PATHOLOGY: C-Reactive Protein

►SpecimenSpecimen: serum : serum ►Reference range:Reference range:

1. < 6 mg/L (aglutination method), 1. < 6 mg/L (aglutination method),

semiquantitativesemiquantitative

2. Immunoturbidimetric (quantitative)2. Immunoturbidimetric (quantitative)

< 5 mg/L< 5 mg/L

C-Reactive ProteinC-Reactive Protein

Page 5: CLINICAL PATHOLOGY: C-Reactive Protein

Method: Latex agglutinationMethod: Latex agglutination

Specimen: SerumSpecimen: Serum

Principle:Principle: Monoclonal anti C-RP will binds and Monoclonal anti C-RP will binds and

agglutinate the CRP in the specimen agglutinate the CRP in the specimen

C-Reactive ProteinC-Reactive Protein

Page 6: CLINICAL PATHOLOGY: C-Reactive Protein

► Allow each component of reagents and Allow each component of reagents and specimen to reach room temperaturespecimen to reach room temperature

► Gently shake the reagentGently shake the reagent► Place a drop of undiluted specimen to the Place a drop of undiluted specimen to the

slideslide► Add one drop of the latex reagent next to Add one drop of the latex reagent next to

the drops of specimenthe drops of specimen► Spread the reagent and specimen over Spread the reagent and specimen over

theentire area of test circle theentire area of test circle ► Gently tilt the test slide backwards and Gently tilt the test slide backwards and

forwards for two minutes.forwards for two minutes.► Positive and negative controls should be Positive and negative controls should be

includedincluded

PROCEDUREPROCEDURE

Page 7: CLINICAL PATHOLOGY: C-Reactive Protein
Page 8: CLINICAL PATHOLOGY: C-Reactive Protein

►Presence of agglutination indicates a Presence of agglutination indicates a level of C-RP in the specimen equal or level of C-RP in the specimen equal or > 6mg/L> 6mg/L

►The lack of agglutination indicates a C-The lack of agglutination indicates a C-RP level < 6 mg/LRP level < 6 mg/L

INTERPRETATIONINTERPRETATION