clinical pathology: c-reactive protein
DESCRIPTION
CLINICAL PATHOLOGY: C-Reactive Protein. GENERAL OBJECTIVE : After finishing lab activity of C-RP, the student will be able to describe C-RP in the DMS case problem SPECIFIC OBJECTIVE : At the end of lab activity of C-RP, the student will be able to interprete: - the normal and abnormal C-RP. - PowerPoint PPT PresentationTRANSCRIPT
CLINICAL CLINICAL PATHOLOGY:PATHOLOGY:
C-Reactive ProteinC-Reactive ProteinGENERAL OBJECTIVEGENERAL OBJECTIVE::After finishing lab activity of C-RP, the After finishing lab activity of C-RP, the student will be able to describe C-RP in the student will be able to describe C-RP in the DMS case problemDMS case problem
SPECIFIC OBJECTIVESPECIFIC OBJECTIVE::At the end of lab activity of C-RP, the student At the end of lab activity of C-RP, the student will be able to interprete:will be able to interprete:- the normal and abnormal C-RP - the normal and abnormal C-RP
C-Reactive ProteinC-Reactive Protein
►C-RP is a serum constituent originally C-RP is a serum constituent originally define by its ability to precipitate define by its ability to precipitate Pneumococcus C polysaccharidePneumococcus C polysaccharide
►C-RP is an acute phase reactant:C-RP is an acute phase reactant:
A protein synthesized by the liver as a A protein synthesized by the liver as a non-specific response to the non-specific response to the inflammationinflammation
►C-RP concentration increased in the C-RP concentration increased in the serum of individu as a response to serum of individu as a response to varies inflammatory condition and varies inflammatory condition and tissue necrosistissue necrosis
►before specific antibody production before specific antibody production starts and the ESR is increased.starts and the ESR is increased.
►After successful therapy, CRP level After successful therapy, CRP level fall before the ESR decreasesfall before the ESR decreases
C-Reactive ProteinC-Reactive Protein
►SpecimenSpecimen: serum : serum ►Reference range:Reference range:
1. < 6 mg/L (aglutination method), 1. < 6 mg/L (aglutination method),
semiquantitativesemiquantitative
2. Immunoturbidimetric (quantitative)2. Immunoturbidimetric (quantitative)
< 5 mg/L< 5 mg/L
C-Reactive ProteinC-Reactive Protein
Method: Latex agglutinationMethod: Latex agglutination
Specimen: SerumSpecimen: Serum
Principle:Principle: Monoclonal anti C-RP will binds and Monoclonal anti C-RP will binds and
agglutinate the CRP in the specimen agglutinate the CRP in the specimen
C-Reactive ProteinC-Reactive Protein
► Allow each component of reagents and Allow each component of reagents and specimen to reach room temperaturespecimen to reach room temperature
► Gently shake the reagentGently shake the reagent► Place a drop of undiluted specimen to the Place a drop of undiluted specimen to the
slideslide► Add one drop of the latex reagent next to Add one drop of the latex reagent next to
the drops of specimenthe drops of specimen► Spread the reagent and specimen over Spread the reagent and specimen over
theentire area of test circle theentire area of test circle ► Gently tilt the test slide backwards and Gently tilt the test slide backwards and
forwards for two minutes.forwards for two minutes.► Positive and negative controls should be Positive and negative controls should be
includedincluded
PROCEDUREPROCEDURE
►Presence of agglutination indicates a Presence of agglutination indicates a level of C-RP in the specimen equal or level of C-RP in the specimen equal or > 6mg/L> 6mg/L
►The lack of agglutination indicates a C-The lack of agglutination indicates a C-RP level < 6 mg/LRP level < 6 mg/L
INTERPRETATIONINTERPRETATION