coliform test

8/6/2019 Coliform Test

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COLOR CHART

many asbubbEs

gel rises \ ,,, ,to surface. -.liquid below-gel is cloudy

indicator -turns yellow

liquid abovegel is clear, J indicatorremains-=-+ red or turns

with fewgas bubblesel remains- at bottomof tube

64-361 6-CC R 9/09


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"LclMotteCHESTERTOWN MARYLAND 21620

COLIFORMINDICATORTEST KIT 4-3616


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The Model TC-5 is an easy-to-use, disposable 5-tube method to indicate the presence ofTotal Coliform Bacteriz in a drinking water supply. The water sample is placed in test vialscontaining the special coliform indicating tablets and stored at room temperature for apredetermined time period. After the required storage period, the vials are examined todetermine the presence of coliform bacteria. The test method and results closely parallel thestandard Total Colitorm Multiple-Tube Presumptive Test (MPN) as outlined in StandardMethods for the Examination of Wa te r and Wastewater.Carefully read through the instructions completely before attempting to collect sample or runthe test.

CONTENTSEach kit contains 5 glass tubes, marked at the 10mL level and containing one Coliform TestTablet. A sterile Water Sampling Bag containing a dechlorinating tablet is included for chlorineremoval.

that may be harmful if misused. Readcaut ions on ~nd~v~duo lonto~nerscarefully. Not to be used by childrenexcept under adult supewlslon


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111111111111111111111I11111I1111111111IINTRODUCTION:T he tab lets in the Model TC-5 contain n utr ients to support the growth of coliform bacteria, agelling substance, and a pH indicator. If coliform organisms are present in t he sample, gas willbe generated as a result of th e bacteria m etabolizing t he n utrie nts in t h e tablet. Thi s gas will betrapped in t he gelling substance and cause th e gel to r ise in th e tube. T he pH indicator maychange co lor from red to yellow as further evidence of co li io m bscteria activity. Suggestion: A sth e test requires a 44-48 hour incubation time, sample at a t i ~ ezriod conve nient to t he userto read the end result.1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I ~ m I I m m I 1 1 I 1 1 ~ 1 I 1 1 mSTORAGET h e unused k it should be s tored at room temperature and QU: ;i 2irect sunlight. Keep awayfrom children. This product is to be used for water analysis ccrrs *_7r?1\-.

CHLORlbOE RE SIDUA L PRE CAU TIONSWate r samples with chlo rine residuals tend to suppress the E:TI- 2- coliform bacteria whenused with this kit. A sterile Water Sampling Bag containir.2 1 :t:?'larinaring age nt is provide dto collect t he sample and neutralize any ch lor ine which rr.2-: ? ;resent in th e water,m


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1111111111111111111111IIIIIIII1IIII1I~1SAMPLE COLLECTION & TEST PROCEDURE1. Determine sarnyle source, 1.e. water spigot, faucet, system sample valve, etc.2. Soak co t ton bal l cr Fauze with househ old alcoh ol and w ipe entire wa ter outlet area of spigot, faucetor test valve. Pa y particular atten tion to faucet aerator screens and mixers.3 . Allow tap (cold m te r ) to run for 2 to 3 minutes or until the line is flushed.4. Reduce tap w a w r 'cu- 10 a rate t ha t will fill the W ater S amp ling Bag slowly withou t splashing. Tearoff top of ba: a: sL:>red l ine and pull tabs outward to open bag. Do n ot touc h bag opening or innersurface.

Caution: Do not allow the tablet to fall out of the bag,m


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5. Fill bag to 4 oz fill line, pull wire ends to close and whirl bag 3 comp lete revolutions. S hak e bag todissolve tablet.

6. Remove a l l 5 tubes from t he display package and remove capsNOTE: Do not remove tabkt from tube.CAUTI0N:To avoid contamination, do not touch the inner surface of the caps, tubes, orhandle tablet


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7. Unwhirl bay and rul ' t a b ouni-ard to open bag. -old one tare n:rs =\yard to form a spout.Carefully till all 5 ~ ~ ' x rO the 10 rnL line withwater sample. " n e ~ ' x eaps tightly.Do not mix or shake tubes.1 8. Stand the car?: ~_rrr:ghtnd place all 5 tubes into I Ithe display rzcJ:r:t. . rubes should now beI standing vertic:::.- a-:-l?he tablet at the bottom ofthe tube. Tab!?: i lie flat on

bottom of tuk.9. Store tubes at r.3:- :zmprature, out of direct sunlight,for 44-48 hours. . t : r remperature should be fairlyconstant and k 7 r t c ~ ' .2'- 85OF.NOTE: Do not ?ii:zi. handle, or shake tubes during thedesignated inc,:T.z:: 7.1 time period. If these storage conditions are not

t followed preci5c:-:. 5 e esults of the test may vary and may not be valid.


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1 m 1 1 1 1 1 1 1 m 1 m m 1 1 1 1 m m 1 1 1 1 1 m 1 1 1 m w m m 1 1 a m m m 1TEST RESULTS

POS ITIVE TEST. ndicator turns yellow.+. Many gas bubbles evident within gelling substance.Gel rises to surface of sample.Substrate below gel is cloudy.PNOTE: Some water samples with a pH below 6 .8 will cause theindicator to tur n yellow prem aturely. T his is not indicative of apositive result. Both the yellow color and gas bubbles must beevident in order to establish a positive test result. Gel will float

Y - 1to surface of sample indicating gas formation. Substrate belowshould be cloudy.


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NEGATIVE TESTIndic ator remains red or turns yellow w ith few gas bubbles.?Gell ing ~ubs ran ce emains o n bottom of tube.. Substrate a h - e gelling substance should be clear.

TNOTE: Some n-zrer samples with a pH below 6.8 will cause the indicator to turn yellowprematurely. T h i s is not indicative of a positive result.


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RESULTS ANALYSISA positive result in any one of the five tubes should be regarded as a potential coliform bacteriacontaminat ion. If a positive result is found in two or more tubes, there is a potential for aserious bacterial con tam inatio n problem. Further steps should be taken to v alidate these resultsby a certified bacteriological laboratory.

Afte r results are recorded, dispose of th e inoculated tubes as follows:Remove al l 5 tubes from display package.O n e tube at a t ime, remove cap and add approximately 1 mL (or 113 teaspoon) ofhousehold bleach (chlorine bleach) to the tube and immediately recap. Follow theabov e procedure for all 5 tubes.. Return tubes to foam tray and again stand upright. Let tubes stand for approximately 4hours.. Dispose of tubes and package as required by local jurisdiction. Do not open tubes orattempt to clean for reuse.

coliform test

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