common sanitization practices andcommon sanitization

SanitizationSanitization

Common Sanitization Practices andCommon Sanitization Practices and Procedures in University Laboratory

Animal ResourcesAnimal Resources

Justin LahmersJustin LahmersAugust 25, 2010ULAR Continuing Education & Professional Development Class

SanitizationSanitization• ALAT 2009 version

– Chapter 4, pages 25 & 26– Chapter 5, pages 31 & 32– Chapter 8

• ALAT 1998 versionALAT 1998 version– Chapter 10

• LAT• LAT– Chapter 7

LATG• LATG– Chapter 5, page 53

Quote of the DayQuote of the Day

• “Keeping oneself and the work• Keeping oneself and the work area neat and orderly is an

ti l t f litessential part of a quality husbandry program. It conveys an attitude of professionalism and enhances sanitation and hygiene programs.”

– Page 72; ALAT Training Manual (1998 edition)

What Are We Going To CoverCover

• Definitions• Definitions

• Products• Products

• Application• Application

• Monitoring• Monitoring

DefinitionsDefinitions

• CleaningCleaning– The complete removal of all visible soil

from the surfacefrom the surface


• SanitizationSanitization– The process by which the number of

bacteria and other organisms living onbacteria and other organisms living on inanimate objects is reduced to an acceptable standard. Sanitization does not necessarily totally eliminate all microorganisms, but is aimed at

d i t t l b f ireducing total numbers of organisms


• SanitizationSanitization– The process involves the removal of

dirt hair saliva blood feces and urinedirt, hair, saliva, blood, feces and urine

– This is followed by washing with– This is followed by washing with detergents and rinsing with a chemical sanitizer or water at 180ºF (82.2ºC)( )


• DisinfectionDisinfection– This process is a more intense form of

sanitation and is designed to reduce tosanitation, and is designed to reduce to a harmless level the number of a specific type of organism, specifically y g ypathogenic organisms (not necessarily spores), on an object

Definitions

• Sterilization

Definitions

Sterilization– In this context

refers to therefers to the process of rendering an gobject totally free of all living

iorganisms

What Do We Sanitize?

• Cages • Sinks

What Do We Sanitize?

Cages• Feeders• Wire bar lids

Sinks• Runs (kennels)• Water bowlsWire bar lids

• Water bottles• Racks

Water bowls• Recoil hoses• Feed bins• Racks

• Carts• Floors walls

• Feed bins• Hoods• Enrichments• Floors, walls,

ceilings (rooms)• Water lines

• Enrichments devices/toys

• Supply and• Water lines• Cage lids

• Supply and exhaust hoses

What Products Do We Use?What Products Do We Use?• STERIS Process NPD• Chlorine Bleach• STERIS Cage-Klenz - (100 150 200)• STERIS Cage-Klenz - (100, 150, 200)• STERIS Foam 140 and 240

STERIS S Kl• STERIS Spor-Klenz• STERIS ALCARE (foam hand rub)• STERIS Kindest Kare (hand and bath

soaps)

What Products Do We Use?What Products Do We Use?

No more Joy in ULAR!

What Products Do We Use?

• STERIS GP Cleaner

What Products Do We Use?

• STERIS GP Cleaner– General cleaning

detergentdetergent

– Ideal for general gcleaning prior to use of a sanitizing

l tisolution

– Multiple dilution rates– Multiple dilution rates depending on use

Considerations Regarding P d t S l tiProduct Selection

• Label claimsLabel claims • Spectrum of activity

Eff ti i h d t• Effectiveness in hard water • Stability of the pH • Use dilution • Contact timeContact time • Temperature

Application MethodsApplication Methods

• Mops (sponge and cotton) andMops (sponge and cotton) and squeegees

• Doodle Bug• Doodle Bug • Sprays• Wiping (rag or cloth) • Immersion • Foaming • Cage washers (high pressure spray)• Cage washers (high pressure spray)• Injectors/proportioner

Process NPD®

Environmental DisinfectantEnvironmental Disinfectant• Germicide Fungicide VirucideGermicide, Fungicide, Virucide,

Detergent, Deodorizer, Concentrate

• QuaternaryAmmonia

Process NPD®

Environmental DisinfectantEnvironmental Disinfectant• Germicide Fungicide and VirucideGermicide, Fungicide and Virucide

– Cide• A suffix that means "a killer of"• A suffix that means a killer of

• DetergentDetergent– a cleansing agent

• Deodorizer– A substance that masks or neutralizesA substance that masks or neutralizes

odors

Process NPD®

Environmental DisinfectantEnvironmental Disinfectant• ConcentrateConcentrate

– material or solution needing dilution

• Use Dilution –Add one-half ounce to each measured gallon of waterof water

Process NPD®

Environmental DisinfectantEnvironmental Disinfectant

Process NPD®

Environmental DisinfectantEnvironmental Disinfectant• Application MethodsApplication Methods

– Cloth, sponge, mop or course sprayContact Time• Contact Time – Allow treated surfaces to remain wet for

ten minutesten minutes

Process NPD®

Environmental DisinfectantEnvironmental Disinfectant• Very ImportantVery Important

– Surfaces must be thoroughly rinsed with water after ten minutes of contact timewater after ten minutes of contact time or dry before animals come into contact with treated surface

– Should not be used for feeders and water bowls

Chlorine to Sanitize BowlsChlorine to Sanitize Bowls

• Sanitize feed and water bowls withSanitize feed and water bowls with chlorine bleach when a cage washer is not availableis not available

P dil ti• Proper dilution– Measure 12 ounces or 1 and ½ cups

bl h ll f tbleach per gallon of water

Chlorine BleachChlorine Bleach

• Used in auto water systems to• Used in auto water systems, to hyper chlorinate water lines on

k d t iti f d dracks and to sanitize feed and water bowls when a cage washer is not available

• Does not contain dirt loosening d t tdetergents

Hyper ChlorinationHyper Chlorination

Water System ChlorinationWater System Chlorination

• To reduce pathogenicTo reduce pathogenic microorganisms in the water supply it can be treated with chlorinecan be treated with chlorine

STERIS Foam ProductsSTERIS Foam Products

• When used with suitable foamingWhen used with suitable foaming spray equipment, these products develop clinging foam to increasedevelop clinging foam to increase cleaning contact time and reduce overall laboroverall labor

Lets CompareLets Compare

Foam 140 Foam 240Foam 140• Alkaline Cleaner• Soil emulsification

Foam 240• Acid-based Cleaner• DescalingSoil emulsification

• Particularly effective against fats, oils and

Descaling• Particularly effective

against hard water scale d i lorganic soils and urine scale

Cage WashingCage Washing

• Uses a combination of water andUses a combination of water and cage cleaning chemistries to sanitize cages racks and accessoriescages, racks and accessories

Cage WashingCage Washing

• Programmable cycles consisting ofProgrammable cycles consisting of prewash, wash, rinse and final 180ºF (82 2ºC) rinse(82.2 C) rinse

Cage-Klenz® 100 Alkaline Cage Wash DetergentCage Wash Detergent

• A liquid phosphate-free detergentA liquid phosphate free detergent system for use in high pressure spray animal cage washing equipmentanimal cage washing equipment

• Removes urine scale, organic soils, and metal oxidesand metal oxides

• Blue label is alkaline

Cage-Klenz® 150 Alkaline Cage Wash DetergentCage Wash Detergent

• A chlorinated alkaline detergent forA chlorinated alkaline detergent for use in automatic washing equipment

• Blue label is alkaline

Cage-Klenz® 200 Acid-based Cage Wash DetergentCage Wash Detergent

• An acid cleaner for removal of a wideAn acid cleaner for removal of a wide spectrum of soils. It contains wetting and penetrating agentsand penetrating agents

• Removes hard water scale, urine scale and metal oxidescale and metal oxide

• Red Label is Acid

Moist Heat SterilizationMoist Heat Sterilization

AutoclaveAutoclave• Definition of autoclave from

Dictionary comDictionary.com– (Noun) an apparatus for sterilizing

objects or for cooking by means ofobjects or for cooking by means of steam under pressure


Autoclave Pressure CookerAutoclave Pressure Cooker

Moist Heat SterilizationMoist Heat Sterilization• Steam and high pressure combine to g p

create superheated steam in excess of 212ºF (100ºC)( )

• Minimum times for a cycle are usually• Minimum times for a cycle are usually 15 minutes at 121ºC (250ºF) at 15 psi

• Flash cycles of 5 minutes at 132.2ºC (270ºF)


• Liquid cycles take longer for theLiquid cycles take longer for the water to heat to the appropriate temperature and they slow exhaust totemperature and they slow exhaust to minimize evaporation loss

STERIS Spor-Klenz RTUSTERIS Spor Klenz RTU

• Sterilant/Sporicidal BactericidalSterilant/Sporicidal, Bactericidal, Turberculocidal, fungicidal, Virucidal Non-Food Contact SanitizerNon Food Contact Sanitizer

A ti i di t• Active ingredients– Hydrogen Peroxide– Peroxyacetic Acid

STERIS Spor-Klenz RTUSTERIS Spor Klenz RTU

• Intended for sterilization orIntended for sterilization or disinfection of stainless steel, plastic items and hard surfacesitems and hard surfaces

• If you put a chemical in a different container you must label the newcontainer you must label the new container

Tracking and EvaluationMethodsMethods

Evaluation MethodsEvaluation Methods

ULAR Form 402ULAR Form 402

ULAR F• ULAR Form 402

R k/T l–Rack/Tunnel Washer T tTemperature Validation FForm

ULAR Form 609 Facility Environmental Testing LogEnvironmental Testing Log

EvaluationEvaluation

• Cage WashersCage Washers– Method: RODAC

• Plastic/polycarbonate and stainless steel• Plastic/polycarbonate and stainless steel sample. Each cage washer – done quarterly

• Room walls/floors after sanitation - random


• RODAC Plates - Replicate OrganismRODAC Plates Replicate Organism Detection And Counting


• SterilizationSterilization– Spore tests each autoclave monthly; dry

goods and liquid cyclegoods and liquid cycle


• Water systemsWater systems– Culture from lines and/or racks done

quarterlyquarterly

Were You Awake?Were You Awake?

True or FalseTrue or False• Sanitization is the process by which

an object is rendered free of all livingan object is rendered free of all living organisms.

F lFalse• Sanitization is the process by which

the number of bacteria and other organisms living on inanimate objects is reduced to an acceptable standard


There is no more Joy in ULAR What isThere is no more Joy in ULAR. What is the STERIS product that is used for general cleaning?general cleaning?

a) Foam 140b) ALCAREb) ALCAREc) GP Cleanerd) Kindest Kare


What is the proper dilution rate forWhat is the proper dilution rate for Process NPD?

a) 12 ounces per gallona) 12 ounces per gallonb) one-half ounce per gallonc) 2 quarts per bucketd) 4 glugs per room) g g p


True or FalseTrue or False• If you put a chemical sanitizer in a

different container you must label thedifferent container you must label the new container?

TTrue


The Environmental Testing Log containsThe Environmental Testing Log contains which of the following?

a) ULAR Form 609 Facility Environmentala) ULAR Form 609 Facility Environmental Testing Log

b) ULAR F 402 W h T tb) ULAR Form 402 Washer Temperature Validation

c) Both of the above

Quote of the DayQuote of the Day

• “Keeping oneself and the work• Keeping oneself and the work area neat and orderly is an

ti l t f litessential part of a quality husbandry program. It conveys an attitude of professionalism and enhances sanitation and hygiene programs.”

– Page 72; ALAT Training Manual (1998 edition)

common sanitization practices andcommon sanitization

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