comparison of planarian (dugesia tigrina) regeneration …comparison of planarian (dugesia tigrina)...
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Comparison of Planarian (Dugesia tigrina) Regeneration Rate in Caffeine and Aloe
vera Solutions
Diane Kwon and Joel TatumBiology 3B
Department of Biological SciencesSaddleback College
Mission Viejo, CA 92692
Planarians (Dugesia tigrina)o Planarians regenerate after cut
279 cuts 2 new planarians identical
to original planarian Same memories
o Planarians sensitive to concentrated solutions Spring water only Tap and DI water may kill
planarians
Caffeine and Aloe verao Caffeine
Stimulant May inhibit cell growth
o Aloe vera Succulent plant Stimulate cell growth
and healing Mucilage tissue (Aloe
vera gel)
HypothesisPlanarian regeneration rate will decrease in caffeine and Aloe verasolutions compared to that of spring water.
Materials and Methodso Planarians purchased from Carolina
Biological Supplies (n=30)o 3 solutions
Arrowhead spring water (control) Caffeine (0.00001 M) Aloe vera (0.00019%)
o Planarians fed 3 days before experiment but not during Proportion-readjustment
regeneration Cells self-destruct to provide
energy/nutrients for regeneration
Materials and Methodso Transverse cut anterior to
pharynxo Observed transected tail
portion Head development more
distinct
o Experiment duration - 23 days Oct 26, 2015 to Nov 18, 2015
Planarian Regeneration1. Stage 1: wound healing
• Neoblasts: population of stem cells
2. Stage 2: blastema formation• Cell proliferation
1
2
Planarian Regeneration3. Stage 3: growth
4. Stage 4: differentiation
3
4
Image from: http://www.extremetech.com/extreme/161092-decapitated-worms-can-regenerate-their-brains-and-the-memories-stored-inside
Resultso Single-factor ANOVA
Bonferroni Correction post-hoc test
o No significant difference (p=0.0726)
Discussiono Caffeine diverts energy from regenerationo Healing properties of caffeine and Aloe vera in lower concentrations
Below lethal limit Potential for growth stimulation Sensitivity of planarians to higher concentration of solutes
o Inconsistent regeneration rate through stages of regeneration
AcknowledgmentsThank you, Professor Teh, for supplies and guidance.
Thank you, Saddleback College Department of Biological Sciences, for providing necessary resources.
Thank you, caffeine, for getting us through the project, even though it inhibited planarian regeneration. (Poor little guys…)