components: a796 dna rehydration solution a797 rnase a

Kit components04.12.2020

Product code Description

A1120 Wizard® Genomic DNA Purification Kit

Components:

A793 Cell Lysis Solution

A794 Nuclei Lysis Solution

A796 DNA Rehydration Solution

A797 RNase A Solution

A795 Protein Precipitation Solution

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Safety data sheetaccording to 1907/2006/EC, Article 31

Printing date 04.12.2020 Revision: 14.08.2020Version number 2.0

51.1.1

* SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifierTrade name: Cell Lysis Solution

Article number: A7931.2 Relevant identified uses of the substance or mixture and uses advised againstNo further relevant information available.Application of the substance / the preparation: Laboratory chemicals

1.3 Details of the supplier of the safety data sheetManufacturer/Supplier:

Promega Corporation2800 Woods Hollow RoadMadison, WI 53711U.S.A.1-608-274-43301-800-356-9526

SDS author: [email protected]

Further information obtainable from:Promega UKDelta HouseSouthampton Science ParkSouthampton SO16 7NSUnited KingdomTel: (44) 23 8076 0225Fax: (44) 23 8076 7014Freephone: 0800 378994E-mail Address: [email protected] Corporation2800 Woods Hollow RoadMadison, WI 53711U.S.A.1-608-274-43301-800-356-9526

SDS author: [email protected] Emergency telephone number:For Chemical Emergency Spill, Leak, Fire, Exposure, or Accident Call CHEMTREC Day or Night Within USAand Canada: 1-800-424-9300Outside USA and Canada: +1 703-527-3887 (collect calls accepted). Within UK +(44)-870-8200418.

GB

(Contd. on page 2)

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Trade name: Cell Lysis Solution

(Contd. of page 1)

51.1.1

* SECTION 2: Hazards identification

2.1 Classification of the substance or mixtureClassification according to Regulation (EC) No 1272/2008The product is not classified as hazardous according to the Globally Harmonised System (GHS).

The product is not classified, according to the CLP regulation.

2.2 Label elementsLabelling according to Regulation (EC) No 1272/2008 Not applicableHazard pictograms Not applicableSignal word Not applicableHazard statements Not applicable2.3 Other hazardsResults of PBT and vPvB assessmentPBT: Not applicable.vPvB: Not applicable.

SECTION 3: Composition/information on ingredients

3.2 Chemical characterisation: MixturesDescription:The product is a mixture of the hazardous substances listed below along with unlisted nonhazardous substances.

Components: Not applicableAdditional information: For the wording of the listed hazard phrases refer to section 15

* SECTION 4: First aid measures

4.1 Description of first aid measuresGeneral information: No special measures required.After inhalation: If the patient feels unwell or is concerned, obtain medical advice.After skin contact: Generally the product does not irritate the skin.After eye contact: Rinse opened eye for several minutes under running water.After swallowing: If the patient feels unwell or is concerned, obtain medical advice.4.2 Most important symptoms and effects, both acute and delayedDizzinessNoneNo further relevant information available.4.3 Indication of any immediate medical attention and special treatment neededNo further relevant information available.

* SECTION 5: Firefighting measures

5.1 Extinguishing mediaSuitable extinguishing agents:CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.5.2 Special hazards arising from the substance or mixtureNone knownNo further relevant information available.5.3 Advice for firefighters No special advice.

(Contd. on page 3) GB

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(Contd. of page 2)

51.1.1

Protective equipment: No special measures required.

* SECTION 6: Accidental release measures

6.1 Personal precautions, protective equipment and emergency procedures Not required.6.2 Environmental precautions: Dilute with plenty of water.6.3 Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).6.4 Reference to other sectionsNo dangerous substances are released.See Section 7 for information on safe handlingSee Section 13 for disposal information.

* SECTION 7: Handling and storage

7.1 Precautions for safe handling No special measures required.Information about fire - and explosion protection: The product is not flammable

7.2 Conditions for safe storage, including any incompatibilitiesStorageRequirements to be met by storerooms and receptacles: No special requirements.Information about storage in one common storage facility: Not required.Further information about storage conditions: None.7.3 Specific end use(s) No further relevant information available.

* SECTION 8: Exposure controls/personal protection

Additional information about design of technical facilities: No further data; see item 7.

8.1 Control parametersIngredients with limit values that require monitoring at the workplace:The product does not contain any relevant quantities of materials with critical values that have to be monitored atthe workplace.Additional information: The lists valid during the making were used as basis.

8.2 Exposure controlsPersonal protective equipmentGeneral protective and hygienic measuresThe usual precautionary measures are to be adhered to when handling chemicals.Respiratory protection: Not required.Protection of hands: Not required.Material of gloves:The selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistanceof the glove material can not be calculated in advance and has therefore to be checked prior to the application.Eye protection: Not required.

GB

(Contd. on page 4)

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(Contd. of page 3)

51.1.1

* SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical propertiesGeneral InformationAppearance:

Form: FluidColour: Colourless

Odour: Not determinedOdour threshold: Not determined.

pH-value at 20 °C: 7

Change in conditionMelting point/freezing point: 0 °CInitial boiling point and boiling range: 100 °C

Flash point: Not applicable

Flammability (solid, gaseous) Not applicable.Decomposition temperature: Not determined.

Auto-ignition temperature: Product is not selfigniting.

Explosive properties: Product does not present an explosion hazard.Explosion limits:

Lower: Not determined.Upper: Not determined.

Vapour pressure: Not determined.

Density at 20 °C: 0.999 g/cm³Relative density Not determined.Vapour density Not determined.Evaporation rate Not determined.Solubility in / Miscibility with

Water: Fully misciblePartition coefficient: n-octanol/water: Not determined.Viscosity:

dynamic: Not determined.kinematic: Not determined.Water: 99.2 %

Solids content: 1.0 %9.2 Other information No further relevant information available.

* SECTION 10: Stability and reactivity

10.1 Reactivity No further relevant information available.10.2 Chemical stabilityThermal decomposition / conditions to be avoided: No decomposition if used according to specifications.10.3 Possibility of hazardous reactions No dangerous reactions known10.4 Conditions to avoid No further relevant information available.10.5 Incompatible materials: No further relevant information available.10.6 Hazardous decomposition products: No dangerous decomposition products known

GB

(Contd. on page 5)

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(Contd. of page 4)

51.1.1

SECTION 11: Toxicological information

11.1 Information on toxicological effectsAcute toxicity Based on available data, the classification criteria are not met.LD/LC50 values relevant for classification: No data availablePrimary irritant effect:Skin corrosion/irritation Based on available data, the classification criteria are not met.Serious eye damage/irritation Based on available data, the classification criteria are not met.Respiratory or skin sensitisation Based on available data, the classification criteria are not met.CMR effects (carcinogenity, mutagenicity and toxicity for reproduction)Germ cell mutagenicity Based on available data, the classification criteria are not met.Carcinogenicity Based on available data, the classification criteria are not met.Reproductive toxicity Based on available data, the classification criteria are not met.STOT-single exposure Based on available data, the classification criteria are not met.STOT-repeated exposure Based on available data, the classification criteria are not met.Aspiration hazard Based on available data, the classification criteria are not met.

* SECTION 12: Ecological information

12.2 Persistence and degradability:Not availableNo further relevant information available.12.3 Bioaccumulative potentialNot knownNo further relevant information available.12.4 Mobility in soil No further relevant information available.Ecotoxical effects:Remark: Not availableAdditional ecological information:General notes: Not known to be hazardous to water.12.5 Results of PBT and vPvB assessmentPBT: Not applicable.vPvB: Not applicable.12.6 Other adverse effects No further relevant information available.

* SECTION 13: Disposal considerations

13.1 Waste treatment methodsRecommendationDisposal should be in accordance with applicable regional, national and local laws and regulations.Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additionalhandling information and protection of employees.European Waste Catalogue (EWC):According to the European Waste Catalogue, Waste Codes are not product specific, but application specific.Waste codes should be assigned by the user based on the application for which the product was used.

Uncleaned packaging:Recommendation: Disposal must be made according to official regulations.Recommended cleansing agents: Water, if necessary together with cleansing agents.

GB

(Contd. on page 6)

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(Contd. of page 5)

51.1.1

* SECTION 14: Transport information

14.1 UN-Number Not hazardous for transportationADR, ADN, IMDG, IATA Not applicable

14.2 UN proper shipping name NoneADR, ADN, IMDG, IATA Not applicable

14.3 Transport hazard class(es) None

ADR, IMDG, IATA

Class Not applicable

ADNADN/R Class: Not applicable

Not regulated

14.4 Packing group NoneADR, IMDG, IATA Not applicable

14.5 Environmental hazards:Marine pollutant: No

14.6 Special precautions for user Not applicable.

14.7 Transport in bulk according to Annex II ofMarpol and the IBC Code Not applicable.

UN "Model Regulation": Not applicable

* SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

Directive 2012/18/EUNamed dangerous substances - ANNEX INone of the ingredients is listed. This product is for research and development use only.

DIRECTIVE 2011/65/EU on the restriction of the use of certain hazardous substances in electrical andelectronic equipment – Annex II

None of the ingredients is listed. This product is for research and development use only.

Classification according to VbF: Not applicable

Waterhazard class: Generally not hazardous for water.15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.

SECTION 16: Other informationThis information is based on our present knowledge. However, this shall not constitute a guarantee for anyspecific product features and shall not establish a legally valid contractual relationship.

Department issuing SDS:Promega CorporationChemical Regulatory Affairs2800 Woods Hollow RoadMadison, WI 53711U.S.A.


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(Contd. of page 6)

51.1.1

Contact:Promega UKDelta House, Enterprise RoadChilworth Science ParkSouthampton SO16 7NSUNITED KINGDOMFree Phone +44 800 559900Free Fax +44 800 181037Abbreviations and acronyms:RID: Règlement international concernant le transport des marchandises dangereuses par chemin de fer (Regulations Concerning theInternational Transport of Dangerous Goods by Rail)ICAO: International Civil Aviation OrganisationADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the InternationalCarriage of Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)VbF: Verordnung über brennbare Flüssigkeiten, Österreich (Ordinance on the storage of combustible liquids, Austria)LC50: Lethal concentration, 50 percentLD50: Lethal dose, 50 percentPBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very Bioaccumulative* Data compared to the previous version altered.

GB

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51.1.1


1.1 Product identifierTrade name: Nuclei Lysis Solution







GB

(Contd. on page 2)

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Trade name: Nuclei Lysis Solution

(Contd. of page 1)

51.1.1





* SECTION 3: Composition/information on ingredients


Components:

CAS: 1185-53-1EINECS: 214-684-5

Tris HCl Acute Tox. 4, H302 1-5%

Additional information: For the wording of the listed hazard phrases refer to section 15




5.1 Extinguishing mediaSuitable extinguishing agents:CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam.


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(Contd. of page 2)

51.1.1

5.2 Special hazards arising from the substance or mixtureNone knownNo further relevant information available.5.3 Advice for firefighters No special advice.Protective equipment: No special measures required.


6.1 Personal precautions, protective equipment and emergency procedures Not required.6.2 Environmental precautions:Dilute with plenty of water.Do not allow to enter sewers/ surface or ground water.6.3 Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).6.4 Reference to other sectionsNo dangerous substances are released.See Section 7 for information on safe handlingSee Section 13 for disposal information.







8.2 Exposure controlsPersonal protective equipmentGeneral protective and hygienic measuresThe usual precautionary measures are to be adhered to when handling chemicals.Respiratory protection: Not required.Protection of hands: Not required.Material of gloves:The selection of the suitable gloves does not only depend on the material, but also on further marks of quality andvaries from manufacturer to manufacturer. As the product is a preparation of several substances, the resistanceof the glove material can not be calculated in advance and has therefore to be checked prior to the application.


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(Contd. of page 3)

51.1.1

Eye protection: Not required.





pH-value at 20 °C: 7.3













10.1 Reactivity No further relevant information available.10.2 Chemical stabilityThermal decomposition / conditions to be avoided: No decomposition if used according to specifications.10.3 Possibility of hazardous reactions No dangerous reactions known10.4 Conditions to avoid No further relevant information available.10.5 Incompatible materials: No further relevant information available.


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(Contd. of page 4)

51.1.1

10.6 Hazardous decomposition products: No dangerous decomposition products known







Uncleaned packaging:Recommendation: Disposal must be made according to official regulations.


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(Contd. of page 5)

51.1.1

Recommended cleansing agents: Water, if necessary together with cleansing agents.





ADR, IMDG, IATA



Not regulated












Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.


Relevant phrasesH302 Harmful if swallowed.


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(Contd. of page 6)

51.1.1

Department issuing SDS:Promega CorporationChemical Regulatory Affairs2800 Woods Hollow RoadMadison, WI 53711U.S.A.Contact:Promega UKDelta House, Enterprise RoadChilworth Science ParkSouthampton SO16 7NSUNITED KINGDOMFree Phone +44 800 559900Free Fax +44 800 181037Abbreviations and acronyms:RID: Règlement international concernant le transport des marchandises dangereuses par chemin de fer (Regulations Concerning theInternational Transport of Dangerous Goods by Rail)ICAO: International Civil Aviation OrganisationADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the InternationalCarriage of Dangerous Goods by Road)IMDG: International Maritime Code for Dangerous GoodsIATA: International Air Transport AssociationGHS: Globally Harmonised System of Classification and Labelling of ChemicalsEINECS: European Inventory of Existing Commercial Chemical SubstancesELINCS: European List of Notified Chemical SubstancesCAS: Chemical Abstracts Service (division of the American Chemical Society)VbF: Verordnung über brennbare Flüssigkeiten, Österreich (Ordinance on the storage of combustible liquids, Austria)LC50: Lethal concentration, 50 percentLD50: Lethal dose, 50 percentPBT: Persistent, Bioaccumulative and ToxicvPvB: very Persistent and very BioaccumulativeAcute Tox. 4: Acute toxicity - oral – Category 4* Data compared to the previous version altered.

GB

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51.1.1


1.1 Product identifierTrade name: DNA Rehydration Solution







GB

(Contd. on page 2)

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Trade name: DNA Rehydration Solution

(Contd. of page 1)

51.1.1













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(Contd. of page 2)

51.1.1











GB

(Contd. on page 4)

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(Contd. of page 3)

51.1.1













Density at 20 °C: 1 g/cm³Relative density Not determined.Vapour density Not determined.Evaporation rate Not determined.Solubility in / Miscibility with






GB

(Contd. on page 5)

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(Contd. of page 4)

51.1.1








GB

(Contd. on page 6)

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(Contd. of page 5)

51.1.1





ADR, IMDG, IATA



Not regulated
















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(Contd. of page 6)

51.1.1


GB

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51.1.1


1.1 Product identifierTrade name: RNase A Solution







GB

(Contd. on page 2)

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Trade name: RNase A Solution

(Contd. of page 1)

51.1.1













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(Contd. of page 2)

51.1.1











GB

(Contd. on page 4)

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(Contd. of page 3)

51.1.1













Density at 20 °C: 1 g/cm³Relative density Not determined.Vapour density Not determined.Evaporation rate Not determined.Solubility in / Miscibility with


dynamic at 20 °C: 0.0952 mPaskinematic: Not determined.

Organic solvents: 0.0 %Water: 99.3 %




GB

(Contd. on page 5)

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(Contd. of page 4)

51.1.1

* SECTION 11: Toxicological information







GB

(Contd. on page 6)

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(Contd. of page 5)

51.1.1





ADR, IMDG, IATA



Not regulated
















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(Contd. of page 6)

51.1.1


GB

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51.1.1


1.1 Product identifierTrade name: Protein Precipitation Solution







GB

(Contd. on page 2)

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Trade name: Protein Precipitation Solution

(Contd. of page 1)

51.1.1





* SECTION 3: Composition/information on ingredients








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(Contd. of page 2)

51.1.1



6.1 Personal precautions, protective equipment and emergency procedures Not required.6.2 Environmental precautions:Dilute with plenty of water.Do not allow to enter sewers/ surface or ground water.6.3 Methods and material for containment and cleaning up:Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).6.4 Reference to other sectionsNo dangerous substances are released.See Section 7 for information on safe handlingSee Section 13 for disposal information.








GB

(Contd. on page 4)

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(Contd. of page 3)

51.1.1





pH-value at 20 °C: 7.5-7.95

Change in conditionMelting point/freezing point: undeterminedInitial boiling point and boiling range: 100 °C












10.1 Reactivity No further relevant information available.10.2 Chemical stabilityThermal decomposition / conditions to be avoided: No decomposition if used according to specifications.10.3 Possibility of hazardous reactions No dangerous reactions known10.4 Conditions to avoid No further relevant information available.10.5 Incompatible materials:Oxidizing agentsStrong acids10.6 Hazardous decomposition products: No dangerous decomposition products known

GB

(Contd. on page 5)

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(Contd. of page 4)

51.1.1

* SECTION 11: Toxicological information







GB

(Contd. on page 6)

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(Contd. of page 5)

51.1.1





ADR, IMDG, IATA



Not regulated












Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water.15.2 Chemical safety assessment: A Chemical Safety Assessment has not been carried out.




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(Contd. of page 6)

51.1.1


GB

components: a796 dna rehydration solution a797 rnase a

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