Compound Light Microscope

•Uses two lenses and has a light source

•Ocular lens usually magnifies 10x

•Can be used to view living cells

•Highest magnification of a compound light microscope is 2,000x

Electron Microscope

• Beam of electrons produce an enlarged image of specimen

•Highest magnification of an electron microscope is 200,000x

• Cannot be used to view living cells

Scanning Electron Microscope

•Electron beam is focused on a specimen coated with a very thin layer of metal

•Electrons that bounce off form an image on a fluorescent screen

•Show 3-D images of cell surfaces

•Black and white images only (artificial colors sometimes added)

Transmission Electron Microscope

•An electron beam is directed at a very thin slice of a specimen stained with metal ions

•Electrons passing through the specimen strike a fluorescent screen, forming an image

•The formed image can show the internal structure of a cell in fine detail

•Black and white images only (artificial colors sometimes added)

Stage

Light Source

Ocular Lens

Objective Lens 10x

Objective Lens 4x

Objective Lens 40x

Course Adjustment

Fine Adjustment

Stage Clips

Base

Body Tube

Arm

Nosepiece

Diaphragm/Condenser

Answers1. Body Tube 12. Coarse

Adjustment2. Nosepiece 13. Fine

Adjustment3. Objective lens 4x 14. Base4. Objective lens 10x5. Objective lens 40x6. Stage Clips7. Diaphragm / Condenser8. Light Source9. Eyepiece10. Arm11. Stage

Magnification

- increase of an objects apparent size.

Resolution

- power to show details correctly

Contrast - the ability to distinguish different densities of structures

Wet Mount

- a glass slide holding a specimen suspended in a drop of liquid