conference on diabetes types of diabetes mellitus type 1 -- insulin dependent type 2 – insulin...
TRANSCRIPT
Conference on Diabetes• Types of diabetes mellitus
type 1 -- insulin dependent type 2 – insulin independent
• Experiment with type 1 genetic or environmental w/immuno-supp
• Assay developmentradioimmunoassay – insulin concentrationshistologic evaluation with Bata cell detectionblood glucose measurementblood antibodies measurement
ISLETS Of LANGERHANS in pancreas
Acinar cells
islet
ISLETS OF LANGERHANS stained with aldehyde-fuchsin, which selectively stains secretory granules of insulin-secreting Beta cells
kidney
Blood sugar concentrations
• Normal range 80 – 120 mg/dl• Diabetic range >120 mg/dl
ROLES AND SPECIFIC ACTIONS OF ANTIOBODIES IN IMMUNITY
COMPLEMENT - MEDIATED LYSISOPSONIZATION - PROMOTE PHAGOCYTOSISTOXIN NEUTRALIZATION
PREVENTION OF MICROBIAL BINDING TO MUCOSAL SURFACE
VIRUS NEUTRALIZATION - INTERFERES WITH CELL PENETRATION
EFFECTOR T CELLS•DELAYED - TYPE HYPERSENSITIVITY T CELLS
CYTOTOXIC CELLS DESTROYS TRANSPLANTED ORGANS
Measure antibodies in blood
radioimmunoassay for insulin blood concentrations