confidential applications for field service engineers
TRANSCRIPT
CONFIDENTIAL
HPLC Applications TrainingMOBILE PHASE CONSIDERATIONS
Flammability Toxicity Miscibility Viscosity Dissolved Salts %Organic Solvent pH (Acid/Alkali) Carbon Dioxide Corrosion
CONFIDENTIAL
HPLC Applications TrainingSome Flammable Solvents Commonly Used Acetonitrile Methanol Hexane, iso-Hexanes, Heptane, iso-Octane Tetrahydrofuran Toluene Acetone Ethyl Acetate Isopropanol
CONFIDENTIAL
HPLC Applications TrainingSome Toxic Solvents Commonly Used Acetonitrile Methanol Hexane Tetrahydrofuran Toluene Dichloromethane (Methylene Chloride) Chloroform Dimethyl Sulphoxide
CONFIDENTIALHPLC Applications TrainingBURDICK & JACKSON
UV Cutoff (nm)
BURDICK & JACKSON
UV Cutoff (nm)
Acetonitrile UV 190 Ethylene Dichloride 228
Water 190 Dichloromethane 233
Hexane UV 195 Chloroform 245
Cyclohexane 200 Ethyl Acetate 256
Heptane 200 Dimethyl Acetamide 268
Isopropyl Alcohol 205 DMF 268
Dimethyl Sulfoxide 268
Methanol 205 Toluene 284
Ethyl Alcohol 210
Trifluoroacetic Acid 210 1,2,4-TCB 308
Tetrahydrofuran UV 212
1,4-Dioxane 215 Acetone 330
Ethyl Ether 215 Methyl Isoamyl Ketone 330
Iso-Octane 215 Methyl Isobutyl Ketone 334
CONFIDENTIAL
HPLC Applications Training HPLC SOLVENTS - UV CUTOFF
Burdick & Jackson solvents are arranged in order of increasing UV cutoff, the wavelength at which the solvent absorbance in a 1 cm path length cell is equal to 1 AU (absorbance unit) using water in the reference cell.
UV Cutoff (nm) Acetonitrile UV 190 Pentane 190 Water 190 Hexane UV 195 Cyclopentane 198 Cyclohexane 200 Heptane 200 Isopropyl Alcohol 205 Methanol 205 Ethyl Alcohol 210 2-Methoxyethanol 210 Methyl t-Butyl Ether 210 n-Propyl Alcohol 210 Trifluoroacetic Acid 210 Tetrahydrofuran UV 212 n-Butyl Alcohol 215 1,4-Dioxane 215 Ethyl Ether 215
CONFIDENTIALHPLC Applications TrainingHPLC Columns
Silica particles (1.5~100μm)
Polymer particles (3~50µm)
CONFIDENTIAL
HPLC Applications Training
SAMPLE PREPARATION
Filtration – Membrane Filters 0.2µm, 0.45µm (Cellulose Acetate, PVDF)
Centrifugation – Economical, less contamination, multiple processing