conifer reproductive cycle -...

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T28 Laboratory Manual Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc. Lab 23 Immature seed cone Scale Ovule Pollen cone Pollen tube Tetrad microspores Megaspore Eggs (within megagametophytes) Pollen (microgametophytes) Zygote Scale Pine seed (with wing) Pollination and Fertilization Meiosis Meiosis Adult sporophyte Young sporophyte Mature seed cone Sperm nuclei Pollen Conifer Reproductive Cycle How to Use the Student Models, continued

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Page 1: Conifer Reproductive Cycle - Hillsboroughsecondary.mysdhc.org/science/documents/Bio1/2011Revisions/Sem 2... · Pollen (mi c rogametophytes) Zygote S c ale ... 6. Inability to prepare

T28 Laboratory Manual

Copyright ©

Glencoe/M

cGraw

-Hill, a division of T

he McG

raw-H

ill Com

panies, Inc.

Lab 23

Imm

atur

ese

ed c

one

Scal

e

Ovul

e

Polle

n co

ne

Po

lle

n t

ub

e

Tetra

d m

icros

pore

s

Meg

aspo

re

Eggs

(w

ithin

meg

agam

etop

hyte

s)

Polle

n(m

icrog

amet

ophy

tes)

Zygo

te

Scal

e

Pine

seed

(with

win

g)

Polli

natio

n an

dFe

rtiliz

atio

n

Mei

osis

Mei

osis

Adul

tsp

orop

hyte Yo

ung

spor

ophy

teM

atur

ese

ed c

one

Sper

m n

ucle

i

Polle

n

Coni

fer R

epro

duct

ive

Cycl

e

How to Use the Student Models, continued

Page 2: Conifer Reproductive Cycle - Hillsboroughsecondary.mysdhc.org/science/documents/Bio1/2011Revisions/Sem 2... · Pollen (mi c rogametophytes) Zygote S c ale ... 6. Inability to prepare

Laboratory Manual T29

Cop

yrig

ht ©

Gle

ncoe

/McG

raw

-Hill

, a d

ivis

ion

of T

he M

cGra

w-H

ill C

ompa

nies

, Inc

.

Lab 23

Clus

ters

of

spor

angi

a

Spor

angi

um

Prot

hallu

s (m

atur

e ga

met

ophy

te)

Mat

ure

spor

ophy

te

Youn

gsp

orop

hyte

Anth

erid

ium

Fron

d

Rhizo

me

Root

sAr

cheg

oniu

m

Egg

Zygo

te

Fertilization

Spor

es

Root

s

Soil

Prot

hallu

s

Meiosis

Mot

ilesp

erm

cel

ls

Egg

cell

Fern

Rep

rodu

ctiv

e Cy

cle

How to Use the Student Models, continued

Page 3: Conifer Reproductive Cycle - Hillsboroughsecondary.mysdhc.org/science/documents/Bio1/2011Revisions/Sem 2... · Pollen (mi c rogametophytes) Zygote S c ale ... 6. Inability to prepare

T30 Laboratory Manual

Copyright ©

Glencoe/M

cGraw

-Hill, a division of T

he McG

raw-H

ill Com

panies, Inc.

Lab 23Meiosis

Fertilization

Spor

e ca

psul

e(s

pora

ngiu

m)

Zygo

te

Gam

etop

hyte

s

Anth

erid

ia

Mal

eFe

mal

e

Mos

s Re

prod

uctiv

e Cy

cle

Mat

ure

spor

ophy

te

Youn

gsp

orop

hyte

Mot

ilesp

erm

Egg

Germ

inat

ing

spor

e

Spor

es

Arch

egon

ia

How to Use the Student Models, continued

Page 4: Conifer Reproductive Cycle - Hillsboroughsecondary.mysdhc.org/science/documents/Bio1/2011Revisions/Sem 2... · Pollen (mi c rogametophytes) Zygote S c ale ... 6. Inability to prepare

194 TEACHER GUIDE AND ANSWERS Laboratory Manual

Copyright ©

Glencoe/M

cGraw

-Hill, a division of The M

cGraw

-Hill C

ompanies, Inc.

Teacher Guide and Answers, continued

Analyze and Conclude 1. These structures are hyphae. They are the fungal

cells. All mushrooms have them. The student’s ability to remove them from the stalk in each sample will depend on the types of mushroom.

2. The hyphae hold the mushrooms in place and help them absorb food or water. Above-level learners may note that fungi secrete enzymes from the hyphae then absorb the digested nutrients. One of the things that distinguishes fungi from other heterotrophs is that they are absorptive.

3. Answers will vary. Students might be able to estimate how many spores are on each radiating line and then multiply by the number of lines in their print. Students should recognize that mushrooms usually grow in moist, warm, and dark places. Spores that do not immediately germinate will remain dormant until the proper growth conditions are met, or they will die.

4. Just as the air in the balloon scattered the cotton balls far away from the balloon, air currents can carry spores far from the mushrooms. Because so many spores are produced, the chances that the spores will land in a favorable environment are great.

5. Answers will vary. Some students might mention that they find a visual display easier to understand.

6. Answers will vary. Students might have found it difficult to identify the parts of a mushroom, or to count the spores; or their balloon might not have spread the cotton balls.

7. The wind would make the model more realistic, spreading the spores even farther from the source. This would also affect the pattern of dispersal.

Inquiry Extensions 1. Common poisonous mushrooms include the fly

argaic and the death cap. 2. Answers will vary. Students who research this

topic will discover general methods for active spore release: the bursting cell, ballistospore discharge, and the catapult or “rounding off.” Students might recognize that, after release, spores can be transported by mammals, birds, insects, leaves, and water. Above-level learners might discover that some spores can survive passage through an animal’s gut, to be dispersed in dung.

Lab 23 • ClassicHow do ferns, mosses, and conifers reproduce?

Objectives• Examine samples of ferns, mosses, and conifers.• Compare characteristics of seeds, spores, and

pollen in each.• Infer how these characteristics have made survival

of each plant possible.

Process Skillsanalyze, examine, compare and contrast, infer

Time Allotment45–60 minutes (45 minutes for lab, an additional 15 minutes for discussion)

Materials fern fronds (with sori) forceps moss sample (with paper towels sporophytes) dropperpine cones (male and water female) petri dishdiagrams of the magnifying lens or life cycles of stereomicroscope, ferns, mosses, and if available conifers colored pencilsscalpel

Preparation• Some students might be allergic to pollen.

Advise those students to take proper precautions (e.g., face masks) before starting the lab.

• Distribute diagrams of the life cycles of mosses, ferns, and conifers from How to Use the Student Models, pp. T24–T31, or some other source. If possible, use color photocopies, posters, colored prints, or artwork.

• If possible, have students examine live plants instead of samples. Going outside to see real examples is preferable.

Page 5: Conifer Reproductive Cycle - Hillsboroughsecondary.mysdhc.org/science/documents/Bio1/2011Revisions/Sem 2... · Pollen (mi c rogametophytes) Zygote S c ale ... 6. Inability to prepare

Laboratory Manual TEACHER GUIDE AND ANSWERS 195

Cop

yrig

ht ©

Gle

ncoe

/McG

raw

-Hill

, a d

ivis

ion

of T

he M

cGra

w-H

ill C

ompa

nies

, Inc

.Teacher Guide and Answers, continued

Teaching the Lab• Ask students to think about what they know about

ferns, mosses, and conifers and the environ ment they grow in before starting the lab. This will help students focus their attention on the differences between these plants.

• Circulate around the room to head off any problems or answer any questions that arise.

Data and Observations 1. Accept all reasonable diagrams. For the fern,

students should draw the underside of the pinna and show the attached sporangia. If moss are in the fruiting stage, students should note the fruiting structure, including the filament, and the sporangium. Male and female pine cones might be attached to the same branch and found in clusters. Generally speaking, the male cones are soft and fleshy, while the female cones are the “classic” hard-finned cone (depending on the stage at which it was harvested).

Female pine cone

Male pine cone

Sporangium

Filament (or stalk)

Gametophyte

Moss fruiting body

Sporophythe

2. Accept all reasonable diagrams. Drawings of the fern spore should show sorus, and if possible, the spores within. Student drawings of the microscopic view of the moss could show the antheridial head, sperm in antheridium, or the ovule in the archegonium. Student drawings of the seeds from a conifer should show the seed body and, depending on the species, any winglike structure used to aid dispersal.

Page 6: Conifer Reproductive Cycle - Hillsboroughsecondary.mysdhc.org/science/documents/Bio1/2011Revisions/Sem 2... · Pollen (mi c rogametophytes) Zygote S c ale ... 6. Inability to prepare

196 TEACHER GUIDE AND ANSWERS Laboratory Manual

Copyright ©

Glencoe/M

cGraw

-Hill, a division of The M

cGraw

-Hill C

ompanies, Inc.

Teacher Guide and Answers, continued

Analyze and Conclude 1. Students should notice the black spots on the

underside of the frond and identify these as spore capsules.

2. Students should notice a lack of seeds in the moss plant. They should see a green, fuzzy plant with tiny rootlike structures called rhizomes.

3. The pine cone might have some leaves, or needles, attached to it. The students might notice the difference between male and female cones. The female cone might have scales with two ovules.

4. The pine tree is a gymnosperm, and it goes through the steps of pollination, fertilization, seed development, and dispersal. The ferns and mosses lack seeds, so they have a different life cycle, which includes the need for water so the sperm can swim to the egg.

5. The fern capsule will develop into a structure with both male and female reproductive structures. The young plant will develop after pollination occurs, whereas the conifer seed is already fertilized. They both contain what is needed for the future generation. The moss sporophyte is similar to the fern capsule in that it contains spores which will be released under the right conditions.

6. Inability to prepare a fine enough section, or lack of discernible structures in the specimens are possible sources of error.

7. Conifers need sandy soil and deep water sources. Ferns and mosses need a moist environment to spread the sperm cells.

Inquiry Extensions 1. Accept all reasonable designs. Students should

make note of the mechanisms (osmosis and diffusion) that allow water and nutrients to reach all portions of the moss.

2. These plants are essential for biodiversity. They serve as producers within the food chain and provide habitats for other creatures. Students might also remark on the plants’ value to humans—for instance, mosses are often used to hold moisture in the soil in gardens or houseplants, or as an emergency bandage for cuts in the skin. Pines are widely used for building materials and furniture. Ferns are considered attractive foliage for houseplants and in floral arrangements.

Lab 24 • Design Your OwnDo plants sweat?

Objectives• Form a hypothesis about how transpiration

is affected by a change in an environmental condition.

• Design an experiment to test the impact of this environmental condition.

Process Skillsform hypotheses, control variables

Time Allotment 45 minutes to set up10 minutes per day for several days

Materialselectric fan small plastic bags food coloring (not the zippered kind)live plant tiespipette petroleum jelly scissors watersmall beaker

Possible HypothesisIf plants are exposed to an environment with more direct sunlight, then the rate of transpiration will increase.

Possible Procedure 1. Choose a location in the classroom that receives

direct sunlight for a majority of the day. If such a location is not available, set up a small desk lamp to simulate the sun.

2. Cover two leaves of the plant with plastic bags. Gently tie the plastic bags around the stem of the leaf.

3. Ensure that one of the leaves is in the direct sunlight.

4. The other leaf covered by the bag needs to be shaded from the sunlight. Cover this leaf and bag with a small lunch bag.

5. During the next few days, check on the plant. Any water that is leaving the leaf though transpiration will collect in the plastic bags as condensation.