conventional pcr: alli real-time pcr: exicycler …...bioneer provides the interchangeable thermal...

Conventional PCR: AllInOneCycler™Real-Time PCR: Exicycler™ 96Protein Synthesis and Purification: ExiProgen™DNA/RNA Preparation: ExiPrep™ 16 Plus & ExiCracker™Electrophoresis: Agaro-Power™Vortex & Centrifugation: ExiSpin™

AllInOneCycler™ PCR System ···············································································································3

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Conventional PCR

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AllInOneCycler™ PCR System

■ Description

AllInOneCyclerTM uses the patented novel alloy block and cutting-edge temperature control algorithm to provide the fastest among the currently released thermal cyclers. This consists of (selectable/interchangeable) 6 different types of block depending on experimental purposes, which are 2 different metals (normal and novel alloy) and classified to 3 sample-well types (96-well, 384-well and slide PCR). There is also precise temperature control system that ensures reproducible results, and the thermal gradient function enables that can be up to 12 different temperatures. Finally, a new graphic interface enables the user to use AllInOneCyclerTM easier with compact and lightweight design and an improved portability

and space efficiency.

■ Features and Benefits

● Fastest ramp rate: A standard thermal block for AllInOneCyclerTM provides

maximum ramp rate at 6.5°C/sec. and the novel alloy thermal block* up to 9.5°C/sec. With this, researchers can save both time and energy along the use of AccuPower® PCR premix technology. Such feature is based on the specific heat capacity of metal alloy that is used in the novel alloy thermal block, which is about 30 % lower than common aluminum block. Thus, the ramp rate of the novel alloy thermal block can go up to 30 % higher than that of aluminum block.

* Novel alloy thermal block: The novel alloy material invented by Bioneer was patented in

Korea and is patent-pending in the United States, Europe, Japan and China.

Figure 1. ‘Specific heat x Specific gravity’ of the materials used in thermal block.

∆T =

* When the amount of heat supplied is constant, the ‘Heat capacity’, ‘Specific heat (C) x Specific gravity (M)’, should be decreased in order to increase temperature change (ΔT).

Q

C×M

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● High energy efficiency

The lower thermal capacity of novel alloy block enables the reduction of electric energy consumption for about 20 % compared to competitor’s instrument in single PCR experiment.

● Excellent Accuracy and Uniformity

Precise temperature control system improves the temperature accuracy and uniformity on the entire thermal block that includes wells between center and border. As a result, the user can achieve reproducible results because of these thermal reliability.

(a) (b)

Figure 2. Comparison of operating times and amplification results between competitor and AllInOneCyclerTM (normal block).

Figure 4. PCR amplification results on 24 representative wells on 96 well-block and 384 well-block to confirm temperature uniformity.

Figure3. (a) Comparison of the relative thermal capacity between normal block and fast block (b) Comparison of electric energy consumption (Single PCR operation) between AllInOneCyclerTM (Fast block) and competitor.

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● Interchangeable thermal block system:

Bioneer provides the interchangeable thermal blocks made of 2 different metals (normal and novel alloy) and classified to 3 sample-well types (96-well, 384-well and slide PCR). The researcher can choose a thermal block among 6 interchangeable blocks for their experimental purposes.

● Thermal gradient PCR:

The researcher can find the optimal experimental conditions easily by using gradient function of AllInOneCyclerTM at once, and also reduce the time and consumables for additional experiments to set optimal condition.

● Easy-to-use interface: Through graphical user interface with 7-inch touch LCD screen, the user can use AllInOneCyclerTM easily and conveniently. In addition, the

user can reduce the time to set PCR protocols by selecting among 8 template protocols supplied in ‘New Protocol’ menu. For high-throughput research, the user can test large amount of samples at the same time by AllInOneCyclerTM PC control software that

operating and monitoring the multiple devices.

● Compact and simple design: Compact and simple design is suitable for small lab space, and easy to move.

(a) (b) (c)

Figure 5. Interchangeable thermal blocks with AllInOneCyclerTM.

Figure 7. Graphic user interface of AllInOneCyclerTM (a) Home screen ; (b) Editing protocol ; (c) Selecting template protocols.

Figure 8. Design of AllInOneCyclerTM.

Figure 6. Amplification results of 830 bp of CSF2 gene in human genomic DNA using the gradient temperature function of the AllInOneCyclerTM to find the optimal PCR condition.

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■ Specifications

Physical specifications

Dimension (W x H x D) 246 x 232 x 369 mm (9.68 x 9.14 x 14.53 in)

Weight 8.4 kg (18.52 lbs)

Display 7-inch Touch LCD screen

Control Embedded Board

Power consumption 100-240 V~, 8.5 A max, 50/60 Hz, 630 VA

Operating temperature 15-30 °C (59-86 °F)

Operating humidity 20-80 %, no condensation

Port 2 USB ports for data storage

Operating specifications

Method of heating / cooling Peltier

Temperature range 4.0 °C-99.9 °C (39.2 °F - 211.82 °F)

Gradient range 20 °C-95 °C

Block type Fast Block Normal Block

Max heating ramp rate 9.5 °C/sec (17.1 °F/sec) 6.5 °C/sec (11.7 °F/sec)

Max cooling ramp rate 7.7 °C/sec (13.86 °F/sec) 4.5 °C/sec (8.1 °F/sec)

Temperature accuracy ± 0.3 °C (±0.54 °F)

Temperature uniformity < 0.3 °C (≤0.54 °F)

Lid temperature 90-110 °C (194 °F-230 °F)

Ramp rate control 1-100%

■ Ordering Information

Cat. no. Product Description

AllInOneCyclerTM PCR System

A-2041-1N AllInOneCyclerTM 96 well PCR system

A-2041-2N AllInOneCyclerTM 384 well PCR system

A-2041-3N AllInOneCyclerTM Slide PCR system

AllInOneCyclerTM Fast PCR System

A-2041-1F AllInOneCyclerTM Fast 96 well PCR system

A-2041-2F AllInOneCyclerTM Fast 384 well PCR system

A-2041-3F AllInOneCyclerTM Fast Slide PCR system

AllInOneCyclerTM Thermal Block

A-2041-1-1 AllInOneCyclerTM 96 well thermal block only

A-2041-2-1 AllInOneCyclerTM 384 well thermal block only

A-2041-3-1 AllInOneCyclerTM Slide thermal block only

A-2041-1-2 AllInOneCyclerTM Fast 96 well thermal block only

A-2041-2-2 AllInOneCyclerTM Fast 384 well thermal block only

A-2041-3-2 AllInOneCyclerTM Fast Slide thermal block only

AllInOneCyclerTM PC Control Software

A-2041-9 AllInOneCyclerTM PC control software

DNA Polymerase & PCR PreMix

K-2012 AccuPower® PCR PreMix, 96 tube

K-2631 AccuPower® ProFi Taq PCR PreMix, 96 tubes

K-2111 AccuPower® Multiplex PCR PreMix, 96 tubes

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K-2611 AccuPower® PyroHotStart Taq PreMix, 96 tubes

Reverse Transcriptase & RT-PCR PreMix

K-2044 AccuPower® CycleScript RT PreMix, dT20, 96 tubes

K-2201 AccuPower® RocketScriptTM Cycle RT PreMix, 96 tubes

K-2501 AccuPower® RocketScriptTM RT-PCR PreMix, 96 tubes

Plastic consumables

TC2-02-N 0.2 ml Flat Cap PCR Tube, 1000 tubes

T-028-CN 0.2 ml PCR 8-Tube Strip with 8-Cap Strip

T-0212-CN 0.2 ml PCR 12-Tube Strip with 12-Cap Strip

3247-01 0.2 ml PCR Tubes with individual attached UltraFlux® I

3420-00 Semi skirt 96-Well PCR Cycle Plate, 10 plates

3430-00 Full skirt 384-Well PCR Cycle Plate, 10 plates

3510-00 96 well sealing mat, 5 ea

Exicycler™ 96 Real-Time Quantitative Thermal Block ·······························································9

Accessories for Exicycler™ 96 ·············································································································13

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■ Description

Exicycler™ 96 is a superior 96-well qPCR system designed for real-time qPCR applications demanding the highest performance. In addition, in vitro diagnostic software packages have also been developed (MFDS, CE-IVD certified versions), which allows the instrument to be used for molecular diagnostic purposes.

■ Features and Benefits● Superior Sensitive Optics by Light Polarization

Bioneer’s imaging technique based on polarization of light provides sensitive detection for robust and reliable results. Our patented polarizing optical apparatus mitigates the common problem of a refection light*, allowing precise quantification and target discrimination. (Figure 1).

*Problem of a reflection light: In the conventional technology, excitation light is generally brighter than the fluorescence generated from the sample. Therefore, light reflected from an optical component interferes with the fluorescence generated from the sample. Furthermore, it is hard to completely distinguish one from the other. This can cause imprecise and unreliable results.

Bioneer’s patented polarizing optics

Light (Unpolarized)

Light (Unpolarized)

Band pass filter Polarizer

Polarizing Beam filter

Polarizer

Band pass filter

DetectorDetector

Band pass filterBand pass filter

Dichromic mirror

Conventional Optics

A re�ection light (Unpolarized)

A re�ection light (Polarlized) Figure 1. Separation of a reflection light from fluorescence.

Noise signal Minimized

noise

Figure 1. Separation of a reflection light from fluorescence.

● Real 5-color MultiplexingExicycler™ 96 is a flexible, 5-color system with advanced optical features (light tunnel technology), which eliminates use of a reference dye. No need to reserve one channel for a reference dye, 5-color multiplexing is available.

Excitation Emission Fluorescence Dye

1 475 nm 530 nm FAM, SYBR Green I

2 520 nm 560 nm JOE, TET

3 550 nm 590 nm TAMRA, CY3

4 570 nm 630 nm Texas Red, ROX, Red 610

5 630 nm 690 nm CY5

● Improved Thermal Block Ramp RateExicycler™ 96 reduces the experimental time due to improved ramp rate compared to the previous model (Figure 2).

Figure 2. Comparison of operating time and ramping time between the previous model and the newest model of ExicyclerTM 96.

Reducingrun time

Tim

e (m

in)

Cycling time

100

80

60

40

20

0

Ramping time

Previousmodel

Newestmodel

Exicycler™ 96 Real-Time Quantitative Thermal Block

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● Excellent Accuracy and Uniformity :

Exicycler™ 96’s precise temperature control system improves the temperature accuracy and uniformity on the whole well of the thermal block. The temperature differences between center and border wells are also remarkably reduced. As a result of the thermal reliability, user can achieve reproducible results (Figure 3). By implementing the in-house designed LT technology in the optics module well-to-well signal variation has been virtually minimized and well-to-well optical homogeneity has been improved. No matter which well the reaction occurs in, the Ct variation will be within 0.3 Ct (Figure 4).

Figure 3. ExicyclerTM 96 has superior well-to-well uniformity.

Figure 4. qPCR result using 1x106 copy of IRF3 gene in each of 96 well positions.

● Self-diagnostics, Automatic Door Open/Close Function, and Intuitive & Convenient Software :

The software of Exicycler™ 96 performs instrument diagnostics before each experiment, preventing run errors from occurring before the experiment and thus saving valuable samples and reagents from going to waste. The loading door is motorized for automatic door opening/closing, which adds convenience and flexibility in operating, as the instrument can easily be incorporated into an automated workflow. A user-friendly interface provides convenience in all steps of the qPCR process including protocol setup, data analysis and result storage. Analysis software that comes with Exicycler™ 96 has 4 different tools - Absolute Quantification, Relative Quantification, Existence/Nonexistence and SNP Genotyping Analysis modules. Users can simply choose an appropriate tool according to their experiment purpose. They can learn how to use the software shortly (Figure 5).

Figure 5. ExicyclerTM 96 Analysis Software.

● Dynamic Range of Detection is wide at over 109 range

Benefit of having a wide linear dynamic range is especially important for the quantification of low concentration target. It makes experimentation easier by avoiding re-assays and dilution issues. Exicycler™ 96 has nine orders of magnitude-wide dynamic range especially for the quantitative experiment (Figure 6).

Figure 6. Ct values of 10-fold diluted samples show a wide dynamic range of quantification and high PCR efficiency.

Fluorescence graph Melting curve analysis

Standard curve analysis Relative Quanti�cation

Existence/non-existence assay SNP Genotyping

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● Fast determination of optimal PCR reaction through tempera-ture gradient function.It is possible to determine the best PCR annealing temperature at once, using temperature gradient function in 40 - 75°C up to

20°C.

● Wide applicationApplication such as touchdown PCR can be performed through controllable function of Time increment or Temp increment.

■ Applications● Quantification of Gene Expression● MicroRNA Expression Analysis● Gene Detection● Virus Load Analysis● Pathogen Detection● Genotype Analysis● SNP (Single nucleotide polymorphism) Detection● Drug Resistance Analysis● Genetic Disease Detection● Oncology● DNA Methylation Study

■ Specifications

Physical specifications

Dimension (W x D x H) 355 x 540 x 470 mm

Weight (kg) 39 kg

Sample Capacity/ Size

Fast block

Opaque White 96-well Low Profile PCR Plate/0.1 ml Opaque White 8-strip Low profile PCR Tube

Normal block

Opaque White 96-well PCR Plate/0.2 ml Opaque White 8-strip PCR Tube

Sample Volume

Fast block 10 - 50 μl(20 μl recommended)

Normal block

20 - 100 μl (50 μl recommended)

Input voltage 100-240 VAC

Frequency 50/60 Hz

Power 800 VA (Fuse: 250 V, F10AL, 2 ea)

Optical Specifications

Light Source Short arc lamp

Wattage 120 W

Sensor 2D CCD

Excitation Filter / Emission Filter 5 Set

Operating Specifications

Method of Heating /cooling Peltier

Temperature Range 4.0-99.9°C (39.2°C - 211.82°C)

Temperature Accuracy ± 0.3°C (±0.54°C)

Temperature Uniformity ± 0.3°C (±0.54°C)

Ramping RateFast block Max 5.0°C (9.0°C)/sec

Normal block Max 4.5°C (8.1°C)/sec

Lid Temperature 90-120°C (194°C - 248°C)

Gradient Range 20-95°C (36°C - 203°C)

Temperature Increment Range 0.1-2.0°C (0.18°C - 3.6°C)

Time Increment Range 1 - 60 sec

Ramp rate control range 1-100%

Operating temperature 15 - 30°C (59°C - 86°C)

Operating Humidity 20 - 80%, no condensation

Computer Specifications

Operating System Window7 (32-bit OS only)

Processor Speed Intel Dual Core E2160 (1.8 GHz) or higher

Memory 1 GB or higher

Communication Port USB 2.0 high speed

Screen Resolution 1280X1024 or higher

* The following specification is subject to modify without any notice.

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A-2060-1 Exicycler™ 96 Real-Time Quantitative Thermal Block

A-2060-2 Exicycler™ 96 Fast Real-Time Quantitative Thermal Block

Related Products

Cat. no. Accessories

A-2060-A1 AccuPower® Fluorescence Test Kit for Exicycler™ 96

A-2060-A2 AccuPower® Fluorescence Test Kit for Exicycler™ 96 Fast

Cat. no. Premix & Reagent

K-6600 AccuPower® Plus DualStar™ qPCR PreMix, 50 µl/rxn, 8-tube strips, 96 rxn, Exicycler™, optical film included

K-6210 AccuPower® GreenStar™ qPCR PreMix, 50 µl/rxn, 8-tube strips, 96 rxn, Exicycler™, optical film included

K-6253 AccuPower® 2X GreenStar™ qPCR Master Mix, 50 µl/rxn, 100 rxn,

K-6704 AccuPower® Dual-HotStart™ RT-qPCR PreMix, 50 µl/rxn, 8-tube strips, 96 rxn, Exicycler™, optical film included

Cat. no. Plastic Consumables

3111-4110 Adhesive Optical Sealing Film, 100 sheets

3111-50 0.2 ml Opaque White 8-tube strip PCR Tube, 250 strips

3111-51 0.2 ml Opaque White 8-tube strip PCR Tube, Low Profile, 250 strips

3111-52 Opaque White 96-well Semi-skirted PCR Plate, 25 plates

3111-53 Opaque White 96-well Semi-skirted, Low Profile PCR Plate, 25 plates (Full-skirted)


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0.2 ml Opaque White PCR 8-tube Strip, 250 strips

■ DescriptionBioneer 0.2 ml opaque white 8-tube strip is made from polypropylene and is specially manufactured to ensure reproducible and sensitive results of Real-Time PCR. The tube strip is compatible with most popular PCR thermal cyclers such as ExicyclerTM 96 and is certified free from RNase and DNase. These tube strips provide higher sensitivity due to improved reflection efficiency as well as increased signal-to-noise ratio compared to natural tubes while preventing well-to-well crosstalk and the loss of fluorescence between the sample and the detector.

■ Specifications

Recommended Rxn Vol. 50 µl

Maximun Capacity 300 µl

Sterility DNase, RNase free

Color Opaque, White

■ Features and Benefits● Optimum for fluorescence detection of Real-Time PCR

reactions or luminescent detections

● Maximize sensitivity and reproducibility of Real-Time PCR

● Compatible with most popular PCR thermal cyclers such as ExicyclerTM 96

● Can be cut and used individually

● Sealable with Bioneer Adhesive Sealing Films or other thermal sealing films using a sealer



3111-50 0.2 ml Opaque White PCR 8-tube Strip, 250 strips

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0.2 ml Opaque White Low Profile PCR 8-tube Strip, 250 strips

■ DescriptionOpaque white Low Profile PCR 8-tube Strip is specialized in small volume qPCR reaction. It provides high efficiency of qPCR reaction when performing below 20 µl volume. The height of tube is shorter than other qPCR tubes, so that it minimizes the condensation on the side wall by eliminating empty space between hot lid and sample. Bioneer’s Opaque white Low Profile PCR 8-tube Strip is made from polypropylene and is specially manufactured to ensure reproducible and sensitive results of Real-Time PCR. The tube strip is compatible with most popular PCR thermal cyclers such as ExicyclerTM 96 and is certified free from RNase and DNase. These tube strips provide higher sensitivity due to improved reflection efficiency as well as increased signal-to-noise ratio compared to natural tubes while preventing well-to-well crosstalk and the loss of fluorescence between the sample and the detector.

■ Specifications




Color Opaque, White

■ Features and Benefits● Optimum for fluorescence detection of Real-Time PCR

reactions or luminescent detections

● Maximize sensitivity and reproducibility of Real-Time PCR.

● Compatible with most popular PCR thermal cyclers such as ExicyclerTM 96

● Can be cut and used individually

● Sealable with Bioneer Adhesive Sealing Films or other thermal sealing films using a sealer



3111-51 0.2 ml Opaque White Low Profile PCR 8-tube Strip, 250 strips

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Opaque White 96-well Semi-skirted PCR Plate, 25 plates

■ DescriptionBioneer's Opaque White 96-well Semi-skirted PCR plate is made of polypropylene material, is especially designed for Real-Time PCR to measure florescence sensitively in result. Opaque White 96-well Semi-skirted PCR Plate can be used the ExicyclerTM 96 and other PCR instrument. Also it has treated with RNase, DNase free to give greater confidence to the user. Compared to the general purpose plate, this Opaque White 96-well Semi-skirted PCR plate has higher signal-to-noise ratio, as well as the reflection efficiency to obtain the higher accuracy of the luminescence measurement. Also the plate prevents crosstalk between each well and light loss between sample and the detector while measuring the fluorescence level by the Real-Time PCR instrument. Each row is marked with alphabets and the column is marked with a number to use conveniently. This semi-skirted plate is much stronger to hold the well's position against vibrations compared to non-skirted plates and gives more accurate results to be obtained.

■ Features and Benefits. ● Maximize sensitivity and reproducibility of Real-Time PCR.

● The alphabet labeled area can be cut out.

● Can be cut to a 24-well or 48-well pieces.

● Optimized for Real-Time response of florescence or luminescence detection.

● Is compatible with ExicyclerTM 96 as well as mostly available PCR instruments.

● Returns the best result when the adhesive sealing film provided Bioneer is used together.

(Third-party adhesive sealing film can be used, but Bioneer sealing film is recommended).

■ Specifications




Color Opaque, White



3111-52 Opaque White 96-well Semi-skirted PCR Plate, 25 plates

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Opaque White 96-well Skirted, Low Profile PCR Plate, 25 Plates (Full-skirted)

■ DescriptionOpaque White 96-well skirted, Low Profile PCR plate is designed to prevent sample condensation on the tube surface.Due to its low profile, it is suitable for the sample volume with less than 20 µl for PCR to obtain higher efficiency for small volume PCR experiment. Due to its low height compared with ordinary plate, the air gap between hot lid and sample is significantly reduced. This reduction in reaction volume due to evaporation can be minimized, prevents sample condensation on the tube surface, and gives the higher efficiency of qPCR results. This Opaque White 96-well skirted, Low Profile PCR plate is much stronger to hold the well’s position against vibrations compared to non-skirted plates and gives more accurate results to be obtained.

■ Features and Benefits. ● Maximized the sensitivity and accuracy of Real-Time PCR.

● Optimized the delectability of fluorescence of Real-Time PCR or luminescent.

● Returns the best result when the adhesive sealing film provided Bioneer is used together.

(Third-party adhesive sealing film can be used, but Bioneer sealing film is recommended)



3111-53 Opaque White 96-well Skirted, Low Profile PCR Plate, 25 Plates (Full-skirted)

■ Specifications




Color Opaque, White

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Adhesive Optical Sealing Film, 100 sheets

■ DescriptionBioneer easy-to-apply thermal stable adhesive sealing film is an optically transparent film with effective and strong adhesive for superior performance in Real-Time PCR. The sealing film has two side tabs for easy application and can be used on any flattop PCR tubes or 96-well plates to prevent evaporation during PCR or Real-Time PCR applications. The sealing film can be removed with no adhesive residue.

■ Features and Benefits● Compatible with any flat tubes, 8-tube strips, or 96-well

plates

● Clear surface for visual sample inspections

● Optimum for fluorescence detection of Real-Time PCR reaction

● Strong to endure thermal cycling processes

● Perfect sealing to prevent evaporation during PCR or Real-Time PCR reaction

● Easy application using side tabs and removal with no adhesive residue



3111-4110 Product Description Adhesive Optical Sealing Film, 100 sheets

ExiProgenTM ·············································································································································19

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Protein Synthesis and Purification

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ExiProgen™

■ Description

Bioneer’s ExiProgen™ is a fully automated system for various scientific purposes in synthetic biology including synthesis of functional proteins, enzymes, biosimilars, and fragments from therapeutic antibodies. ExiProgen™ is able to synthesize and purify up to 16 proteins in a single run. Simply, plasmid DNA or linear PCR product is added to the system followed by the synthesis of protein from Bioneer’s E. coli extract for the transcription and translation processes. Target proteins are further purified by Ni-NTA magnetic beads in the system using his-tag on the protein. ExiProgen™ yields up to 100 μg of protein with the purity of >90% in 6 hrs. This versatile system also allows purification of nucleic acids from various sources including tissue, blood, bacteria, and plant. ExiProgen™ system will offer strong solutions for your research including drug discoveries.

■ Features and Benefits● Easy and Fast Protein Synthesis and Nucleic Acid Extraction with

High Purity: With template DNA and an ExiProgen™ EC kit, the ExiProgen™

synthesize and purify the target proteins with over 90% of purity within 6 hrs. Also with ExiPrep™ Plus Kits, ExiProgen™ enables preparation of high-quality DNA/RNA from various samples such as whole blood, bacteria, plant, etc.

● Built-in Protocols Optimized for Protein Synthesis/Purification and Nucleic Acid Extraction:

ExiProgen™ has built-in protocols optimized for protein synthesis/purification and nucleic acid extraction. This user-friendly interface provides reproducible and reliable results. The instrument software is also able to be upgraded automatically through the network connection.

● Temperature Controlled Block: Temperature Controlled Block allows the target protein to be

synthesized at a set temperature ranging from 4 to 90°C, and an eluted sample to be kept at low temperature to maintain protein function.

● Magnetic Block & Heating Block: ExiProgen™ is equipped with a patented magnet block and a

heating block to enhance purification efficiency. The magnetic block holds magnetic beads tightly during wash and elution steps, which minimizes loss of beads and enables inhibitors such as ethanol to be removed completely. The heating block controls a temperature strictly, which provides reliable and reproducible results for protein synthesis.

● Contamination Shield: Contamination shield is designed to protect the assay from

cross-contamination during instrument operation. Whenever the pipette tips are moving, the contamination shield is always co-located under the tips, therefore eliminating the possibility of cross-contamination which is necessary when working with multiple samples.

● Easy-to-use LCD Touch Screen: The 3.5” touch screen maximizes efficiency by offering an intuitive

interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.

● UV Lamp: ExiProgen™ is equipped with a powerful UV sterilization lamp

that enables the user to sterilize the instrument chamber before and/or after experiment. This removes possible inter-assay cross-contamination that may occur.

■ Functions

Protein Expression O

Protein Purification O

Nucleic Acid Extraction O

TFT Touch Screen O

UV sterilization O

Heating Block & Magnetic Block O

Contamination Shield O

TCP/IP Network Connection O

Temperature Controlled System O

Automatic Dispensing O

ExiProgen™

■ Specifications

Dimensions (W x H x D) 320 x 500 x 535 mm

Weight 27 kg

Sample Number 1-16 Samples

Temperature Range 15-30°C

Humidity Range 20-80%, no condensation

Operating System Stand alone (Built-in)

User Interface 320 x 240 touch screen graphic LCD

AdaptorAC Input 100 – 240 V~, 2.3 - 0.8 A, 50/60 Hz

24 VDC, 7.5 ADC Output

Instrument DC Input 24 VDC, 7.5 A

UV Sterilization 15 minutes cycle

Communications TCP/IP

Heating Temperature 40-95°C

*The following specification is subject to change without notice.

■ Application● Protein & Enzyme engineering● Protein evolution● Synthetic biology● Bio-energy R&D● Protein drug R&D

■ Available Kits from Bioneer● Please refer “J. Protein Expression & Purification” section

■ Experimental Procedure

■ Experimental Data

Figure 1. PAGE data of various proteins synthesized from various templates. Up to 16 types of proteins can be expressed and purified simultaneously with an average of over 90% purity. Amount of loading sample is 1/35 of total sample.1: Calul3 (17.5 kDa) 2: RNase H (20 kDa) 3: DUSP 3 (22 kDa) 4: CAT (24 kDa) 5: AcGFP (29 kDa) 6: EF-Ts (34 kDa) 7: VF (45 kDa) 8: Poly A polymerase (50 kDa) 9: M-MLV RTase (75 kDa) 10: BM3 (117 kDa)M2: AccuLadderTM Protein Size Marker (Broad)

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A-5041 ExiProgenTM

Cat. no. Protein synthesis and Purification Kits for ExiProgenTM

K-7300 ExiProgen™ EC Protein Synthesis Kit, 16 rxns



K-7310 ExiProgen™ EC-Maxi Protein Synthesis Kit, 8 rxns

K-7320 ExiProgen™ EC-Tagfree Protein Synthesis Kit, 8 rxns

K-7330 ExiProgen™ EC-Disulfide Protein Synthesis Kit, 8 rxns

K-7220 ExiProgen™ His-tagged Protein Purification Kit, 16 rxns

K-7221 ExiProgen™ His-tagged Protein Purification Kit, 32 rxns

K-7240 ExiProgen™ Dialysis Kit, 16 rxns

Cat. no. Nucleic Acid Extraction Kits for ExiProgenTM

K-4211 ExiPrep™ Plus Blood Genomic DNA Kit, 96 rxns

K-4212 ExiPrep™ Plus Tissue Genomic DNA Kit, 96 rxns

K-4214 ExiPrep™ Plus Bacteria Genomic DNA Kit, 96 rxns

K-4215 ExiPrep™ Plus Plant Genomic DNA Kit, 96 rxns

K-4244 ExiPrep™ Plus Plant Total RNA Kit, 96 rxns

K-4271 ExiPrep™ Plus Viral DNA/ RNA Kit, 96 rxns

K-4272 ExiPrep™ Plus Viral DNA Kit, 96 rxns

K-4273 ExiPrep™ Plus Viral RNA Kit, 96 rxns

ExiProgen™

ExiPrep™ 16 Plus ····································································································································23

ExiCracker™ NEW ·······································································································································25

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DNA/RNA Preparation

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ExiPrep™16 Plus

■ Description

ExiPrep™ 16 Plus is an automated sample prep system to extract DNA or RNA from various biological samples including cell culture, whole blood, serum, plasma, swab, bacteria, yeast, etc. The ExiPrep™ 16 Plus can handle up to 16 samples at once and includes optimized extraction protocols for many sample types so you get the unsurpassed results from your nucleic acid. The ExiPrep™ 16 Plus has advanced features such as an intuitive touch screen, UV sterilization cycle, and an unique contamination shield, each designed to provide an extra level of

confidence in your results.

■ Features and Benefits● DNA/RNA Preparation from Various Sample: With ExiPrep™ Plus Kits, ExiPrep™ 16 Plus enables preparation of

high-quality DNA/RNA from various samples such as whole blood, bacteria, plant, etc.

● Built-in Protocols Optimized for Nucleic Acid Extraction: ExiPrep™ 16 Plus has built-in protocols optimized for nucleic acid

extraction. This user-friendly interface provides reproducible and reliable results. The instrument software is also able to be upgrad-ed automatically through the network connection.

● Combined Magnetic and Heating Block: The ExiPrep™ 16 Plus has a combined magnetic block and heating

block to increase efficiency of nucleic acid extraction. The block re-duces experiment time and increases elution efficiency, resulting in increased yield of nucleic acid extraction.

● Contamination Shield: Contamination shield is designed to protect the assay from cross-

contamination during instrument operation. Whenever the pipette tips are moving, the contamination shield is always co-located under the tips, therefore eliminating the possibility of cross-contamination which is necessary when working with multiple samples.

● Easy-to-use Touchscreen Interface: The 3.5” touch screen maximizes efficiency by offering an intuitive

interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.

● Automatic UV Sterilization Lamp: The ExiPrep™ 16 Plus has a powerful UV sterilization lamp that

enables the user to sterilize the instrument chamber before and/or after experiment. This prevents possible inter-assay cross-con-tamination that may occur.

■ FunctionsDNA/RNA Extraction O

TFT Touch Screen O

UV Sterilization O

Heating Block & Magnetic Block O

Contamination Shield O

■ Specifications


Weight 27 kg

Sample Number 1 - 16 samples

Temperature Range 15-30°C

Humidity Range 20-80%, no condensation

Operating System Stand alone (Built-in)

User Interface 320 x 240 touch screen graphic LCD

AdaptorAC Input 100–240 V, 2.3-0.8 A, 50/60 Hz

24 VDC, 2.7 ADC Output

Instrument DC Input 24 VDC, 2.7 A

UV Sterilization 15 minutes cycle

Communications TCP/IP

Heating Temperature 40-95°C

* The following specification is subject to modify without any notice.

■ Application● Gene Expression Study ● Genetic Engineering ● GMP& Food poisoning test ● Biological terror detection

■ Available Experiments with Bioneer’s Kits● Blood Genomic DNA Extraction Kit● Tissue Genomic DNA Extraction Kit● Bacterial Genomic DNA Extraction Kit

● Plant Genomic DNA Extraction Kit

● Plant Total RNA Extraction Kit

● Viral DNA/RNA Extraction Kit

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ExiPrep™ 16 Plus

■ Procedure


Figure 1. Electrophoretic analysis of genomic DNA extracted from the whole blood. It is the result of extracted genomic DNA from 200 μl of whole blood, quantified 100 ng per lane on 1% agarose gel.

Figure 2. Result of real-time PCR of genomic DNA extracted from whole blood. This figure shows result of real-time PCR performance using five primer and probe kit which is related to alzheimer disease.



A-5030 ExiPrep™ 16 Plus

Cat. no. Nucleic Acid Extraction Kits for ExiPrep™ 16 Plus

K-4211 ExiPrep™ Plus Blood Genomic DNA Kit, 96 rxns

K-4214 ExiPrep™ Plus Bacteria Genomic DNA Kit, 96 rxns

K-4215 ExiPrep™ Plus Plant Genomic DNA Kit, 96 rxns

K-4244 ExiPrep™ Plus Plant Total RNA Kit, 96 rxns

K-4271 ExiPrep™ Plus Viral DNA/ RNA Kit, 96 rxns

K-4272 ExiPrep™ Plus Viral DNA Kit, 96 rxns

K-4273 ExiPrep™ Plus Viral RNA Kit, 96 rxns

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ExiCracker™

■ Description

ExiCracker™ is a tool which can crush plant sample such as seed, leaf and root. Its simultaneous 16-sample handling makes it faster and more convenient than pestle. It confers lower possibility of sample loss or contamination. Also ExiCracker™-processed sample can be used directly for ExiPrep™ automatic sample prep system or for MagListo™ 5M Plant Genomic DNA Extraction Kit.

■ Features and Benefits● Maximum 16 samples at once: All 16 samples in sample rack are crushed at the same time

through pestle moved by handle. It makes faster and more convenient pretreatment of plant samples possible.

● Crushing of seeds is never been easier: Crushing of large, hard seeds (pumpkin, sunflower & rice) or of

leaves (broccoli & cabbage) become easier.

● Resistant to Corrosion: It is made of alloy metal with special treatment. Therefore

ExiCracker™ is resistant to crack as well as to corrosion by reagent for nucleic acid extraction, which confers long lifetime.

■ Specifications


Weight 6.5 kg

No. of wells 16 wells

Diameter of wells 8 mm

■ Available Experiments with Bioneer’s Kits● ExiPrepTM Plus Plant Genomic DNA Kit

● ExiPrepTM Plus Plant Total RNA Kit

● MagListoTM 5M Plant Genomic DNA Extraction Kit

■ Procedure


Figure 1. Comparison between ExiCracker™ and a mortar.



A-5030-A21 ExiCrackerTM

Agaro-Power™ ········································································································································27

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Electrophoresis

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Agaro-Power™

■ Description

AgaroPower™ is an agarose gel electrophoresis device with an attached loading adapter so even novices can load up to 96 samples (excluding size markers) within 2 - 3 minutes. The device is composed of a durable polycarbonate material that displays superior durability compared to acryl-based electrophoresis systems. The power supply and gel casting tray are included for easy operation and gel casting.

■ Features and Benefits● Power Supply:

Compact in design, it allows low (75V) or high voltage (150V) and is controlled with a simple ON/OFF system.

● Loading Adaptor: With the loading adaptor, samples can be easily and quickly

loaded without damaging the gel loading wells using an 8- or 12-channel pipette. (Up to 96 samples loading wells can be used).

● Gel Caster & Tray: You can make a gel with uniform thickness easily using

graduated gel caster and tray.

● Comb set Comb Set provided with the device consists four combs, and

each comb has two different well on either side as 25/34 well, 13/34 well, 15/34 well, and 21/34 well.

■ Specifications

Distance Between Electrodes 228 mm

Buffer Volume 700 ml

Gel Tray Size (L x W) 163 x 149 mm

Comb (No. of teeth)

25 well or 34 well/line x 1ea




Caster Size (L x W) 170 x 156 mm

Input Voltage110 VAC 50/60 Hz, 220VAC 50/60 Hz

Output VoltageHigh:150 V±10% Low : 75 V±10% (User selectable)



A-7020 Agaro-PowerTM System

Cat. no. Plastic Consumables

A-7020-1 Agaro-PowerTM, Power Supply

A-7020-2 Agaro-PowerTM, Agar Tank/Lid

A-7020-3 Agaro-PowerTM, Comb Set

A-7020-3-1 Agaro-PowerTM, Comb (25 well / 34 well)




A-7020-4 Agaro-PowerTM, Gel Caster/Tray

A-7020-5 Agaro-PowerTM, Cable Jack

ExiSpin™ ··················································································································································29

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Vortex & Centrifugation

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ExiSpin™

■ Description

ExiSpin™ has the functions of a vortexer as well as a microcentrifuge in single unit, and will significantly save your time in the lab. ExiSpin™ comes with two rotors; one for 4 x 8-tube strip and the other for 12 x 1.5/2.0 ml tubes. Use it for minipreps, resuspension of oligos, preparation of your PCR reaction, or any task that requires vortexing in micro test tube or 0.2 ml PCR tubes.

■ Features and Benefits● Fully Automated Spin-Mix-Spin Technology:

The time and strength of centrifugation/mixing is fully programmable. The numbers of spin-mix-spin cycles can be set (up to 999). After setting single click of button automatically starts the process as follows:

1st spin: Spin down sampleVortexing of samples2nd spin: Spin down mixed sample

● Convenience and High Reproducibility:

ExiSpin™ provides reproducible results using protocols optimized to samples by preventing the possibilities of experimental errors. Four 8-strips tubes can be handled simultaneously, which enable 32 PCR/qPCR mixture tubes ready to run at once. Twelve 1.5 ml tubes can be loaded for simultaneous cell lysis and subsequent centrifuge-down.

● Economical and Efficient:

ExiSpin™ has both a centrifuge and vortex function. You can save not only your time but also the budget by purchasing one instead of two separate instruments. 12 x 1.5 ml rotor and 4 x 8-strip rotor can be easily exchanged.

<Presented rotor for 8-tube strip PCR tube [left] and 1.5 ml microtube [right]>

■ Application

● Bacterial cell lysis● Mixing with sample and PCR/qPCR PreMix● Restriction digest, kinase reaction, ligation reaction● Experiment that need to repetitive mixing and spin-down

■ Specifications

Dimensions (W x H x D) 19 x 23.5 x 12.5 cm

Weight 2.7 kg

Spin Regulation1,000 - 3,500 rpm (increment 100 rpm)

Spin Timer 1 sec- 30 min

Vortexing Strength Soft, Medium, Hard

SMS- Cycle Regulation 1 - 999 cycles

Power Supply AC 24 V, 1,250 mA



A-7040 ExiSpinTM

conventional pcr: alli real-time pcr: exicycler …...bioneer provides the interchangeable thermal...

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