corporate capabilities 2018 - pharmtechfiles.pharmtech.com/alfresco_images/pharma/2019/01/... ·...

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Corporate Capabilities

2018A Guide to Leading Separation Science Suppliers

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© 2014 Hamilton Company. All rights reserved.

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More than 50 years of precision liquid handling experience. Proven accuracy. Continuous research and development. Choose an autosampler syringe from Hamilton Company and be confident in your results.

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2017 - 03495

4 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 WWW.CHROMATOGRAPHYONLINE.COM

UBM Americas (www.ubmlifesciences.com) is a leading worldwide media com-pany providing integrated marketing solutions for the Fashion, Life Sciences and Powersports industries. UBM Americas serves business professionals and consum-ers in these industries with its portfolio of 91 events, 67 publications and directories, 150 electronic publications and Web sites, as well as educational and direct market-ing products and services. Market leading brands and a commitment to delivering innovative, quality products and services enables UBM Americas to “Connect Our Customers With Theirs.” UBM Americas has approximately 1000 employees and currently operates from multiple offices in North America and Europe.

© 2017 UBM. All rights reserved. No part of this publication may be reproduced or

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visit http://www.copyright.com online. For uses beyond those listed above, please

direct your written request to Permission Dept. fax 440-756-5255 or email: Maureen.

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UBM Americas provides certain customer contact data (such as customer’s name,

addresses, phone numbers, and e-mail addresses) to third parties who wish to pro-

mote relevant products, services, and other opportunities that may be of interest to

you. If you do not want UBM Americas to make your contact information available

to third parties for marketing purposes, simply call toll-free 866-529-2922 between

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phone 218-740-6477.

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of information supplied herein or for any opinion expressed.

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tel. (218) 740-6831, e-mail [email protected].

REPRINTS: Reprints of all articles in this issue and past issues of this publication

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(440) 891-2742, fax (440) 891-2650, or e-mail [email protected].

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Corporate CapabilitiesCorporate Capabilities2 0 1 8

Corporate Profiles

8 ACCTA, Inc.

9 Antec Scientific

10 Advanced Chemistry

Development, Inc. (ACD/Labs)

12 Biotage, LLC

13 CDS Analytical

14 CEM Corporation

15 CTC Analytics

16 Chem Service, Inc.

18 FMS, Inc.

19 Fritsch Milling & Sizing, Inc.

20 Hamilton Company

21 Imtakt USA

22 LECO Corporation

24 MACHEREY-NAGEL GMBH &

CO. KG

25 Markes International

26 MicroSolv Technology

Corporation

28 MilliporeSigma

29 Mott Corporation

30 Neta Scientific Inc.

31 Parker Hannifin

32 PerkinElmer, Inc.

33 PHOTONIS USA

34 PSS Polymer Standards

Service Inc.

35 Molex

36 Postnova Analytics Inc.

37 Proton OnSite

38 Quantum Analytics

39 Restek Corporation

40 Sciencix

41 Shodex/Showa Denko

America, Inc.

42 Shimadzu Scientific

Instruments

44 SilcoTek® Corporation

45 Sorbent Technologies, Inc.

46 Tosoh Bioscience LLC

47 UCT, Inc.

48 Valco Instruments Co., Inc.

(VICI)

50 Wyatt Technology Corp.

52 YMC America, Inc.

54 ZirChrom Separations, Inc.

Application Notes

55 Medical/Biological

58 Food & Beverage

62 Environmental

63 Pharma/Drug Discovery

64 Polymer

65 General

DECEMBER 2017 LCGC NORTH AMERICA CORPORATE CAPABILITIES 5www.chromatographyonline.com

SPONSORED BY

Current Trends in Chiral SeparationsA Q&A

A discussion of the trends, development, and use of

chiral stationary phases and separation modes

Chiral compounds such as pharmaceuticals, pesticides, and raw materials are

challenging to work with because enantiomeric species can provide varying physio-

chemical properties. Chromatography products and systems are available for use in a

range of separation modes to assist in the accurate separation and analysis of these distinct

compound forms. LCGC recently sat down with Marc Jacobs, PhD, to discuss trends in the

development of chiral columns and his advice on choosing columns and separation modes

for chiral screening.

LCGC: How did you initially become interested in the field of chirality?Jacob: Early in my academic career in France, I was a student of Professor Pierre Duhamel,

a pioneer of asymmetric synthesis. I was introduced to the concept of chirality in one of his

classes. My PhD thesis was on the topic of asymmetric synthesis of amino acids, which are

important chiral natural products. In France, chirality has historical roots from long ago when

Louis Pasteur separated tartaric acid using a microscope. I’ve been involved in the field of

chiral analysis for the last 20+ years.

LCGC: Why is there so much emphasis on chiral analysis and the detection of enantiomers in pharmaceutical products?Jacob: Chiral purity is particularly important in pharmaceuticals. Because pharmaceutical prod-

ucts are developed for use in humans, you must ensure that the purity of the pharmaceutical

ingredient is well understood and characterized. Since the 1990s, the FDA has required the

analysis of the enantiomers for a chiral drug in development. Many drugs before the 1990s

were developed as racemates. Pharmaceutical analysts found that sometimes an enantiomer,

also called a distomer, can have no activity, and in some cases, can have toxic activity. The

good enantiomer is referred to as the “eutomer.”

The case of thalidomide raised the importance of chiral analysis. The drug was used in the

1950s as a sedative and to alleviate morning sickness in pregnant women. One of the enantio-

mers of the drug, however, possessed some adverse properties, and many children were born

with severe deformities. After the discovery of these effects, the FDA required the analysis of

chiral drugs for all of the existing enantiomers to determine whether or not toxic properties exist.

LCGC: What recent improvements have been made in the kinds of chiral columns in use today for analytical and preparative separations?Jacob: Most of the major developments in chiral chromatography took place in the 1980s

with the introduction of the polysaccharide-based chiral stationary phase, which is the most

useful phase for analytical and preparative separations. In the 1980s, just two different types of

phases were used, which were based on a cellulose backbone or an amylose backbone. Now,

we have a dozen or more stationary phases. Current developments are focused on generating

different chiral selectors on those polysaccharide-based stationary phases.

Marc Jacob, PhD

Global Product Manager for Chiral and Preparative

Chromatography Phenomenex

CURRENT TRENDS IN CHIRAL SEPARATIONS

About 10 years ago, the immobilized version of polysaccharide-

based stationary phases was introduced. These phases were

coated on the silica material, which created issues with the

solubilization of the chiral selector during a chromatographic

analysis. The current immobilized version is robust and

opened up opportunities for chiral separations because it

allows for the use of more types of organic solvents. With

a chiral separation, you cannot predict which phase will

separate the two enantiomers. Typically, quite a few columns

and mobile phases must be screened to obtain good results.

LCGC: Do you still see a need for both coated and immobilized polysaccharide chiral columns?Jacob: My visits to customers indicate that coated phases are

not disappearing and are useful in terms of improving success.

Because you cannot predict the separation, you need a high

success rate with the column or columns being used. Analysts

continue to use both coated and immobilized columns in their

screens, especially in SFC, as well as in normal-phase and

reversed-phase chromatography. Immobilized phases are of

specific interest because they give you access to a new chiral

selector and enhance your chance of finding a successful chiral

separation.

LCGC: What separation modes are most widely used for chiral separations?Jacob: Historically, normal-phase mode has been used, which

is a mixture of an alkane and alcohol, for example, hexane and

ethanol or hexane and IPA. The polar organic mode (POM),

which is a mixture of two organic solvents, has also been

used. POM uses methanol and acetonitrile or ethanol and

acetonitrile in the mobile phase, which are compatible with

coated or immobilized phases. The variety of solvents that

could be used was limited originally because primarily coated

stationary phases were used.

Approximately 90% of chiral separations are carried out on

polysaccharide-based phases. In the last 20 years, super-

critical fluid chromatography (SFC) has become prominent

because of its speed. With SFC, there is less viscosity from

the solvent in the mobile phase, so separations can be run

at higher flow rates—almost four times faster—which results

in faster screening. SFC mobile phases contain at least

60% carbon dioxide (CO2), so there is less mobile phase to

evaporate, which is important in preparative chromatography.

Analytical-scale separation use much less solvent overall,

which makes it a “green” technology. Analytical separa-

tions are powerful using polysaccharide-based columns in

reversed-phased mode. Analysts thought these phases would

not be stable, but reversed-phase separations using water/

acetonitrile or water/methanol demonstrate good results, and

their use is growing.

We did some work on FMOC amino acids, which are

an important chiral raw material as a building block for the

synthesis of peptides. Reversed-phase chromatography was

able to separate all of the natural protected amino acids. With

immobilized chiral stationary phases, a variety of solvents

can be considered. In the polar organic mode, tetrahydrofuran

(THF) can be mixed with ethyl acetate or MTBE with methanol—

many combinations are possible. This type of approach is not

common, however, and it is expensive to use these organic

solvents. Consequently, historically, normal phase has been

used most commonly for chiral analysis, with SFC being used

for preparative separations and in pharmaceutical analysis,

and reversed-phase for analytical methods.

LCGC: What recommendations do you have for sta-tionary phases and modes to start with when under-taking chiral LC screening projects?Jacob: I will address small molecules because we’re dis-

cussing chiral separations of pharmaceuticals, which gener-

ally have only one chiral center. Three to six chiral stationary

phases will yield about a 90% success rate in screenings.

I recommend Lux Amylose-1 and Cellulose-1, which

cover the polysaccharide backbones. These phases were

developed in the 1980s by Yoshio Okamoto and commercial-

ized. Another option is a chiral selector with an ester, called

Cellulose-3, that gives you another type of interaction with your

molecule. I would also include a chlorinated phase, Cellulose-4

or Cellulose-2, which were developed by Professor Bezhan

Chankvetadze, a collaborator of Phenomenex. I suggest a

couple of immobilized phases because these chiral selec-

tors are unique and not available as coated phases, like the

i-Cellulose-5. These six phases should give you almost 99%

success, but just Amylose-1, Cellulose-1, and Cellulose-3 will

give you about 75% success.

Regarding mode, I suggest SFC, if possible, for speed, as

well as polar-organic, reversed-phase, and normal-phase

modes depending on your equipment. In sum, three to six

phases and a couple of separation modes.

LCGC: Have you seen an increase in chiral separations in any industries other than pharma?Jacob: We see some chiral analysis of basic chemical raw

materials such as FMOC amino acids, which are a critical raw

material made all over the world. Chiral analysis is also used

in the agrochemical industry for chiral pesticides, herbicides,

and fungicides. Because these products are used in food,

it’s critical to determine whether there are adverse effects

stemming from their chemistry. Some chemicals used in the

electronics industry are chiral and require analysis and clas-

sification. Most of the pharmaceutical drugs that are chiral

are now being developed as pure enantiomer and require

a battery of tests. But it remains to be seen whether the

development of small molecule drugs will increase or stay

stable in the future as other types of drug are developed in

the pharmaceutical industry.

8 LCGC NORTH AMERICA CORPORATE CAPABILITIES DECEMBER 2017 www.chromatographyonline.com

ACCTA, Inc.

3534 Jessie Ct.

St. Paul, MN 55125

T E L E P H O N E

(651) 731-3670

F A X

(651) 730-0965

E - M A I L

[email protected]

W E B S I T E

www.accta.com

N U M B E R O F E M P L O Y E E S

2

Y E A R F O U N D E D

1993

ACCTA, Inc. meet the unique goals of our clients and

give them a competitive edge. Customers

tell us that they can learn as much in four

days, working with us, as they would in four

months of unproductive trial-and-error effort

on their own. Our program includes:

⦁ Laboratory consulting: Our experience

in laboratories over three decades can

solve your HPLC method development,

method optimization, and laboratory or

method problems. ACCTA’s experience

will help you become a more strategic

laboratory operation. You will save at least

one month of time for each day we spend

working together. The solutions that come

from our consultative efforts are designed

to deliver significant and lasting benefits

to your lab, even after we leave.

⦁ Staff Training: “Good training is your

best lab instrument.” ACCTA’s experi-

enced laboratory consultants will help

your staff operate a chromatography in-

strument and understand the results. We

can make that learning easier and faster

with basic or advanced level hardware

training, covering HPLC, UHPLC, and GC.

We are ready to help you implement

customized ChemStation training from

experts who have used the software in

many different applications. Discover

how our flexible approach and innovative

thinking can benefit your laboratory op-

erations and increase your profitability.

⦁ Lab Life-Line: We know every lab is dif-

ferent and that’s why we’ve customized

each of our services to provide fact-based

analysis and strategies exclusively for your

individual needs. Our extensive experi-

ence and ability to get the job done effi-

ciently and cost-effectively are why we are

consistently selected to deliver obtainable

and sustainable improvements including:

reducing solvent costs, improving your

laboratory’s efficiency, data processing,

reporting times, staff mentoring, workflow

optimization, and more. Our goal is to

always leave our clients in a better place

than where we found them.

Column-Screening Facility The ACCTA laboratory provides HPLC

method development feasibility information

and specialized chemical analysis capabili-

ties. This rapid screening approach can help

get you started in the right direction, saving

you time and money.

Company Description ACCTA provides elite-level consulting and training services

to analytical laboratories. We specialize in making your

(laboratory) life easier!

⦁ Is your lab fully utilizing its staff and equipment?

⦁ Do you need new or better procedures and processes?

⦁ Are you looking for a range of technical support options?

Since 1993 we have been developing analytical solutions for our

customers’ analytical problems. These solutions produce stress-

relief and significant return on investment (ROI) for laboratories.

Chief Chromatographic Techniques Supported ⦁ HPLC

⦁ UHPLC

⦁ GC

⦁ GPC–SEC

Markets Served ⦁ Pharmaceutical

⦁ Food

⦁ Consumer products

⦁ Nutritional supplements

⦁ Chemicals and chemical additives

⦁ Petroleum

⦁ Independent laboratories

⦁ Government

⦁ Academia

⦁ Legal

⦁ And More!

Major Products/Services ACCTA associates have worked as consultants in the laboratory

for decades. No project is too small or too large—our team of

leading experts has the depth of knowledge to scale services to


Antec Scientific

One Boston Place,

201 Washington Street,

Boston, MA 02108

T E L E P H O N E

(888) 572-0012

E - M A I L

[email protected]

W E B S I T E

www.antecscientific.com


1990, since 2010 in USA

Antec Scientific Electrochemical Reactors

for MS and Synthesis

With the introduction of the ROXY™ EC

system, REDOX reactions can be monitored

directly by Mass Spectrometry (MS).

The on-line coupling of Electrochemical

Reactors with MS (EC/MS) has found

many application areas from proteomics,

lipidomics, drug metabolism, drug stability

testing, to environmental degradation.

Another emerging application of Antec’s

flow and batch reactors is in Flow Chemistry

for rapid synthesis of drugs, metabolites,

degradants (reference materials).

SenCell™ for Detection

Proprietary Adjustable Spacer Technology

(AST) for highest sensitivity (US Patent

9,310,330)

μ-PrepCell 2.0™ for Reactions

Redox reactions upfront MS: disulfide

bond reduction in proteins, mimicking

drug metabolism, drug stability testing/

degradation

SynthesisCell™ for Synthesis

Batch reactor for rapid synthesis of mg

quantities of products

Company Description For over 27 years Antec Scientific has been the world

leading supplier of analytical instrumentation based on

Electrochemistry (EC). Antec’s line of instruments include

Electrochemical Detectors (ECD), Analyzers built on HPLC-

ECD and Electrochemical Reactors for use with MS (EC/MS)

and Synthesis.

Electrochemical Detectors for HPLC

The DECADE™ line of ECDs (Elite™ and Lite™) have become the

benchmark in detection and can be used with any third party

(U)HPLC system. Different flow cells are available to cover a

broad range of applications from the analysis of antibiotics to

sugars or pharmaceuticals.

Dedicated Analyzers

The ALEXYS™ Analyzers based on HPLC with ECD are turnkey

instruments built for highest performance and to ensure ease-

in-use. Guaranteed applications have been developed with

the ALEXYS Neurotransmitter Analyzer for sensitive analysis in

microdialysates and brain tissue. Other application areas are

in clinical/diagnostics, food/beverage, environmental, and

drugs/pharmaceuticals analysis.


Advanced Chemistry

Development, Inc.

(ACD/Labs)

8 King Street East, Suite 107

Toronto, Ontario, Canada

M5C 1B5

T E L E P H O N E

+1 4163683435

E - M A I L

[email protected]

W E B S I T E

acdlabs.com


1994

Major Products/Services ⦁ ACD/AutoChrom—comprehensive

method development and optimization

software that supports a Quality by

Design (QbD) approach based on

chromatographic principles. From

strategy creation and investigation

of maximum chromatographic space

to execution of screening runs and

identification of the optimal method.

Optional instrument control for Agilent

ChemStation and Waters Empower.

⦁ ACD/LC Simulator—method simulation

software for multivariate parameter

optimization. Optimize gradient, solvent

ratio and concentration, temperature,

and pH. Also provides retention

prediction and automatic peak

matching between experimental runs.

⦁ Luminata—software for the efficient

and comprehensive management

of impurity data. Establish effective

process and analytical impurity

control strategies based on live

chromatographic and spectral data and

route knowledge, in a purpose-built

environment.

Advanced Chemistry Development, Inc. (ACD/Labs)

Company Description Advanced Chemistry Development, Inc. (ACD/Labs), is an

informatics company that develops and commercializes

solutions in support of R&D. Our software platforms unify

analytical and chemical information in a collaborative

environment; and provide tools that enable scientists to

extract, capture, and apply knowledge from analytical

experiments and predicted molecular property data. We

integrate with existing informatics systems and undertake

custom projects including enterprise-level automation.

We offer more than two decades of success helping

organizations accelerate R&D and leverage corporate

intelligence. ACD/Labs provides worldwide sales and support.


⦁ UHPLC

⦁ LC–MS

⦁ GC–MS

⦁ LC–MS/MS

Markets Served ACD/Labs tools support R&D in various industries including

pharma and biotech, chemicals, consumer goods, agrochemi-

cals, petrochemicals, academic, and government institutions.

www.acdlabs.com/autochrom

Software to streamline method

development using QbD principles

Direct Instrument Control

Agilent Chemstation & Waters Empower

Automated Peak Tracking

Follow all components between runs

Robust Methods through Simulation and Experimentation

Optimize multiple parameters simultaneously

Complete Data Management

Seamlessly store, retrieve, and organize methods and results

Comprehensive Reporting

Communicate easily

CCoCCCCoMa

Robust Methods through

veengg

ilyy

Better Methods, Faster

ACD/AutoChrom


Biotage, LLC

10430 Harris Oaks Blvd. Ste C,

Charlotte, NC 28269

T E L E P H O N E

(704) 654-4900

F A X

(704) 654-4917

E - M A I L

[email protected]

W E B S I T E

www.biotage.com


USA 75

Elsewhere 250


1989

Biotage, LLC

Major Products/Services Sample Prep, Flash Chromatography,

Automation, Evaporation, Microwave

Synthesis, Peptide Synthesis

Facility Biotage USA, Biotage Sweden, Biotage UK,

Biotage Korea, Biotage Japan, and Biotage

China

Company Description Biotage offers a range of separation and synthesis technologies

across the three product areas Organic Chemistry, Analytical

Chemistry, and Industrial Products to a global market. The

Biotage products rationalize the workflow of customers and

reduce their impact on the environment, for example by using

a lower volume of solvents. Customers use Biotage products

in their development of new medicines and to analyze

samples from hospital patients, in forensic laboratories, or

for the analysis of environmental and food samples. Biotage

also offers products to remove undesired substances from

pharmaceuticals during the manufacturing process.

Chief Chromatographic Techniques Supported ⦁ Sample Prep

⦁ Flash Chromatography

⦁ Protein Precipitation

⦁ Supported Liquid Extraction

⦁ Solid Phase Extraction

⦁ Filtration

⦁ Automation

⦁ Evaporation

⦁ Microwave Synthesis

⦁ Peptide Synthesis

Markets Served Clinical, Forensic, Tox, Pharmaceutical, CRO, Biotech, Food,

Agricultural, Environmental, Government, Chemicals, Educa-

tion, Natural Products, Petroleum


Major Products/Services 6th generation Pyroprobe 6000 series, 2nd

generation Purge and Trap autosampler

7350/7450 with 7000E concentrator, 2nd

generation Thermal Desorption autosampler

7550 with 9300 series concentrator, 2nd

generation Thermal Desorption stand-alone

autosampler 7550S with Peltier supported

Facility CDS’s state-of-art 10,000 sq. ft. facility

in Oxford, Pennsylvania, houses

manufacturing, customer support,

engineering, service and administrative

personnel in one building, where we

design, build, assemble, and analytical

test everything in house. Our production

utilizes six sigma lean manufacturing

processes and is delivering the products

with the highest quality in the industry.

AnalyticalCDSCDS

CDS Analytical

Company Description CDS Analytical is a leading global provider of innovative ther-

mal sample preparation instrumentation for the analytical

laboratory. For over 45 years our exclusive focus has been on

conceiving, designing, manufacturing, and supporting leading

edge instruments. CDS offers a complete suite of diverse front-

end GC equipment including pyrolyzers, purge and trap, head-

space, and thermal desorption systems. With a well-established

leadership role with pyrolyzers and other introduction systems,

as well as broad market penetration on a global scale, CDS

continues to be innovative and dynamic, and will pioneer the

next wave of sample introduction instrumentation.

Chief Chromatographic Techniques Supported⦁ Pyrolysis

⦁ Purge and Trap

⦁ Dynamic Headspace

⦁ Thermal Desorption

Markets Served CDS Analytical products have been serving but not limited to the

following markets as: Polymer, Environmental, Aerospace, Agri-

culture, Automobile, Chemical Weapon, Energy, Food and Flavor,

Forensics, Paper & Ink, Pharmaceuticals, Semiconductor, Textiles,

and Tobacco.

CDS Analytical

465 Limestone Road,

Oxford, PA 19363

T E L E P H O N E

(800) 541-6593

F A X

(610) 932-4158

E - M A I L

[email protected]

W E B S I T E

www.cdsanalytical.com


23


1969


Major Products/Services CEM has recently launched a revolutionary

new system for LC–GC sample prep.

The EDGE Energized Dispersive Guided

Extraction system provides fully automated

extraction in as little as 5 min; including

filtration, cooling, and system washing.

The EDGE combines the processes

of Pressurized Fluid Extraction with

Dispersive Solid Phase Extraction in a

single instrument to yield fast results,

regardless of sample size, matrix, or use

of sorbents. The EDGE supports a wide

variety of applications including pesticides

in foods, total fat extraction, phthalates

in plastics, and environmental extractions

such as EPA 3545.

Facility CEM’s global headquarters, research

laboratories, and manufacturing facilities

are located in Matthews, North Carolina.

The company has subsidiaries in the

United Kingdom, Ireland, Germany, Italy,

France, and Japan as well as more than 50

distributors worldwide.

CEM Corporation

Company Description CEM Corporation is a leading global company specializing

in scientific solutions for critical laboratory applications. We

design and manufacture systems for analytical laboratories,

bioscience applications, life sciences, and processing plants

worldwide. Our product portfolio includes innovative

instrumentation for sample preparation for elemental or

chromatographic analysis, chemical synthesis, peptide

synthesis, and other applications.

Chief Chromatographic Techniques Supported ⦁ LC

⦁ LC–MS

⦁ GC

⦁ GC–MS

Markets Served CEM instrumentation is used by chemists, scientists, and

technicians in private industry as well as leading universities,

analytical laboratories, and research facilities around

the world. We support numerous markets including the

pharmaceutical, chemical, environmental, applied materials,

food, and petroleum industries, among others.

CEM Corporation

PO Box 200,

Matthews, NC 28106

T E L E P H O N E

(704) 821-7015

(800) 726-3331

F A X

(704) 821-7894

E - M A I L

[email protected]

W E B S I T E

www.cem.com


USA: 245

Elsewhere: 65


1978


Major Products/Services PAL System is the tool box for sample

preparation, from a simple liquid injection

to complete workflows. A PAL System can

be adapted or extended to meet almost

any requirement.

CTC Analytics

Company Description CTC Analytics, a privately owned Swiss company, is leading

in front-end automation for gas and liquid chromatography.

Over the last 30 years, investments in the continuous devel-

opment of a flexible, highly reliable, advanced laboratory sam-

ple handling platform have been made, primarily designed for

use in the pharmaceutical, life science, chemical, environmen-

tal and food & flavor industries.

Our robotic autosampler systems have a flexible modular

design, allowing our worldwide customer base to create their

own customized solution to respond to their individual needs. All

products are distributed through OEM channels and a selected

network of independent CTC distributors around the world.

Chief Chromatographic Techniques Supported ⦁ GC

⦁ GC–MS

⦁ LC

⦁ LC–MS

Markets Served ⦁ Environmental

⦁ Food

⦁ Pharmaceutical

⦁ Chemical

⦁ Forensic

⦁ Clinical

CTC Analytics

Industriestrasse 20, 4222

Zwingen, Switzerland

T E L E P H O N E

+41 61 765 8100

E - M A I L

[email protected]

W E B S I T E

www.palsystem.com


1985


Chem Service, Inc.

660 Tower Lane,

West Chester, PA 19381

T E L E P H O N E

(800) 452-9994

F A X

(610) 692-8729

E - M A I L

[email protected]

W E B S I T E

www.chemservice.com


1962

Chem Service, Inc.

agencies; food quality control; agricultural

testing and research; life science endeavors;

environmental control and remediation;

college and university work; and the

manufacture of chemicals, pulp and paper,

and alcoholic beverages.

Major Products/Services ⦁ Flame retardants/PBDE

⦁ Metabolite standards

⦁ EPA 535 ESA/OA metabolites

⦁ Explosive residues

⦁ PCB congeners

⦁ Petroleum hydrocarbons

⦁ Fatty acid methyl esters

⦁ High purity inorganics

⦁ Surfactants

⦁ Anti-oxidants

⦁ Ultraviolet inhibitors

⦁ Plasticizers

⦁ Biological stains

⦁ Phthalate standards

⦁ Polynuclear aromatics

⦁ Vitamins

⦁ Carbohydrates standards

⦁ Forensic standards

⦁ Personal care product standards

⦁ Pesticide residue standards

Facility Chem Service’s headquarters are located in

West Chester, Pennsylvania. A global network

of distributors for sales outside the United

States is available for all of our products.

Company Description Established in 1962, Chem Service is accredited for ISO Guide

34:2009 and ISO/IEC 17025:2005 and registered and certified

to the ISO 9001:2008 Quality Management System for the

design, development, production, distribution, and servicing of

organic neat and synthetic reference materials. Chem Service, Inc.

produces high purity chemicals for use as reference materials and

for other laboratory purposes. More than 95% of their standards

grade materials have a certified purity of 98.0% or greater and

do not require purity corrections when preparing solutions for

use with EPA, USTM, UST, and numerous other international

methods. Standards grade chemicals are clearly labeled with an

expiration date that is based on years of experience in handling

and testing. Products are packaged in small quantities to

minimize storage, waste, and disposal requirements. Organic and

inorganic chemicals, solutions, and mixtures are available to meet

a wide range of specialized laboratory needs.

Chief Analytical Techniques Supported⦁ GC–FID

⦁ GC–ECD

⦁ GC–TCD

⦁ GC–MS

⦁ HPLC

⦁ FT–IR

⦁ UHPLC–MS

Markets Served Chem Service has thousands of worldwide customers in a

wide range of sectors, including government and regulatory

chemservice.com | [email protected] | 1-800-452-9994

QUALITY

,62Guide 34 - 9001 - 17025

Pesticide and Metabolite Standards CatalogPPeessticiide and Metabolite Standards CaatallooggPPeessticiide and Metabolite Standards Caataloogg

REFERENCESTANDARDS

CERTIFIED

Over 2,000 products

Cannabis StandardsNeonicotinoidsPBDEsPhthalatesVitaminsExplosivesand more!ł

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FMS, Inc.

580 Pleasant Street

Watertown, MA 02472

T E L E P H O N E

(617) 393-2396

F A X

(617) 393-0194

E - M A I L

[email protected]

W E B S I T E

www.fms-inc.com


90

O U T S I D E U S

4


1985

FMS, Inc.

Chief Chromatographic Techniques Supported ⦁ Automated sample preparation

⦁ Sample extraction

⦁ Pressurized liquid extraction

⦁ Solid phase extraction

⦁ Automated fractionation

⦁ Sample cleanup

⦁ Automated sample cleanup

⦁ Multicolumn cleanup

⦁ Concentration

⦁ Evaporation

Markets Served ⦁ Agricultural

⦁ Chemical

⦁ Clinical

⦁ Coatings

⦁ Cosmetics

⦁ Energy

⦁ Environmental

⦁ Flavors

⦁ Food

⦁ Life science

⦁ Pharmaceutical

⦁ Petrochemical

⦁ Tobacco

⦁ Toxicology

⦁ Utilities

Facility Based in Watertown, Massachusetts,

just outside Boston, FMS, Inc. owns and

operates out of its 150,000 sq. ft facility on

the banks of the Charles River. All FMS, Inc.

products are proudly manufactured in the

US. The facility houses sales, marketing,

manufacturing, a machine shop, ISO

17925 accredited laboratory, and R&D.

Company Description Founded in 1985, Fluid Management Systems, Inc. designs,

manufactures, and supports analytical instruments used

by scientists to perform extraction, cleanup, fractionation,

and concentration of samples prior to chemical analysis.

Some of the industries that rely on our sample preparation

systems are: agricultural, chemical, clinical, coatings,

cosmetics, energy, environmental, flavors, food, life science,

pharmaceutical, petrochemical, tobacco, toxicology, and

utilities. Automated sample preparation systems from

FMS are designed to replace outdated, labor-intensive

techniques that hinder laboratory productivity. Our Total

Sample Preparation systems improve the efficiency of both

laboratory personnel and expensive instrumentation. Our

automated sample preparation systems allow laboratory

personnel to reduce costs, shorten sample turn around

time, and improve the quality and consistency of their

results by eliminating the variability inherent with manual

sample preparation methods. All FMS consumables are

guaranteed and manufactured in Class 10,000 and Class

1000 cleanroom environments and both our instruments

and consumables are proudly made in the US. We support

our sample preparation systems with an ever-increasing

network of field sales, service, and applications expertise.

FMS systems are sold worldwide by a direct sales force,

independent representatives, and distributors. After 25 years

in business, FMS has established itself as the world leader in

providing automated sample preparation systems in the field

of persistent organic pollutants (POPs) analysis.


Fritsch Milling & Sizing, Inc.

Company Description FRITSCH is an internationally recognized manufacturer of

application-oriented laboratory instruments for sample

preparation and particle sizing.

Chief Chromatographic Techniques SupportedLaboratory Mills for optimal sample preparation of

chromatographic techniques

Markets Served Worldwide in the following industries: Mining, Construction

materials, Biology, Chemistry, Mechanochemistry,

Agriculture and forestry, Geology/mineralogy, Ceramics/glass

industry, Plastics/textiles, Foodstuffs,

Metallurgy, Environment, Pharmaceuticals

Major Products/Services Particle size reduction, milling, grinding, and analysis of

particle size and shape.

Facility USA headquarters and applications laboratory in North

Carolina. Global headquarters in Germany. Sales offices in

China, Asia Pacific, Russia, and France.

Fritsch Milling

& Sizing, Inc.

57 Grant Drive Suite G,

Pittsboro, NC 27312

T E L E P H O N E

(919) 229-0599

E - M A I L

[email protected]

W E B S I T E

www.fritsch-us.com


100+ worldwide


1920-Fritsch GmbH


pH stability (0–14) with the pressure stabil-

ity of silica-based columns. With Hamilton’s

Polymeric Reversed Phase (PRP™) HPLC

columns and resins, the sample dictates the

necessary separation conditions, not the

limitations of the column. Hamilton can also

customize HPLC columns to meet a desired

application need. Each product is manufac-

tured to achieve the highest level of accuracy.

Chromatography Syringes – Hamilton of-

fers the most complete selection of syringes

on the market for use in various applications

including GC and HPLC (autosamplers and

manual injection), thin layer chromatogra-

phy (TLC), liquid handling, and life sciences.

Crafting exceptional syringes is an evolving

science, which is why Hamilton is dedicated

to the continuous research and development

of this product line. Hamilton constantly en-

riches its entire syringe offering by either im-

proving existing models or introducing new

ones. Using customers’ needs and feedback

as a guide, we innovate in ways that maxi-

mize flexibility, performance, and value.

Microlab® 600 Diluter/Dispenser – The

Microlab® 600 is a highly precise syringe

pump with a touchscreen interface designed

to quickly and easily dilute and dispense flu-

ids, while increasing throughput and reduc-

ing cost and wasted buffer. This positive dis-

placement system provides better than 99%

accuracy, independent of a liquid’s viscosity,

vapor pressure, and temperature. The inert

fluid path minimizes sample carryover and is

compatible with harsh chemicals.

Facilities Hamilton Company is a global enterprise with

headquarters in Reno, Nevada; Hopkinton,

Massachusetts; and

Bonaduz, Switzer-

land, and subsidiary

offices throughout

the world.

Hamilton CompanyChromatography Products for HPLC and GC

Company Description Hamilton Company is an industry leader in the design and

manufacture of liquid handling, process analytics, robotics, and

automated storage solutions. For 60 years, Hamilton has been

satisfying customer needs by combining quality materials with

skilled workmanship to ensure the highest level of performance.

Hamilton’s lifelong commitment to precision and quality has

earned a global ISO 9001 Certification.

Founded on the technology of analytical Microliter™ and

Gastight® syringes, Hamilton has a broad offering of laboratory

products including manual and semi-automated precision fluid

measuring instruments, chromatography products, process

sensors, laboratory electrodes, pipettes, and more.


⦁ Polymeric HPLC columns: reversed phase, anion exchange,

cation exchange, and ion exclusion

⦁ Silica HPLC columns

⦁ GC septa

⦁ Chromatography syringes (HPLC and GC)

Markets Served ⦁ Analytical

⦁ Pharmaceutical

⦁ Biotechnology

⦁ Industrial

⦁ Clinical

⦁ Environmental

Major Products/Services HPLC Columns – Hamilton manufactures 18 types of polymeric

HPLC columns for reversed phase, anion exchange, cation ex-

change, and ion exclusion separations, providing a wide range

of retention characteristics and performance benefits. Hamilton’s

polymer-based HPLC columns provide maximum inertness and

Hamilton Company

4970 Energy Way

Reno, NV 89502

T E L E P H O N E

(775) 858-3000

(800) 648-5950

F A X

(775) 856-7259

E - M A I L

[email protected]

W E B S I T E

www.hamiltoncompany.com


1953


Major Products/Services Below is a sample of some HPLC column

phases from Imtakt:

⦁ Dacapo DX-C18: 2.5 μm particle C18 col-

umn designed with a revolutionary dual

matrix particle technology, optimized for

extreme pH conditions.

⦁ Intrada Amino Acid: The world’s first

mixed-mode column designed to sepa-

rate amino acids without the need for any

derivatization.

⦁ Intrada SEC: Silica based SEC column

designed for use in SEC-MS compatible

conditions. GPC and GFC.

⦁ SCHERZO SM-C18: C18 multi-mode

column with both anion and cation ex-

change.

⦁ PRESTO FF-C18: Consists of C18 bonded

to monodispersed 2 μm non-porous

silica. It is useful for both biopolymer and

synthetic polymer separations. Ideal for

intact antibody and ADC analysis.

⦁ CADENZA CD-C18: High resolution; 25–

50% lower pressure; best with hydropho-

bic compounds, molecular recognition,

and small molecule isomer separation.

⦁ Cadenza HS-C18: High resolution C18

column designed to accept direct injec-

tion of biological samples. No need to

protein precipitate your samples for high

throughput early drug discovery or clinical

lab testing.

⦁ UNISON UK-AMINO: Revolutionary aque-

ous durability for aminopropyl phase;

aqueous to non-aqueous normal phase

separation.

Facility We are located in Portland, Oregon, and

deliver next day for 95% of North Ameri-

can orders and always within one week.

Imtakt USA

Company Description Our HPLC columns have been designed and manufactured

by Imtakt Corporation with exceptional craftsmanship and

decades of industry experience. Truly some of the most unique

column chemistries in the market today. Our columns give

chromatographers:

⦁ High resolution

⦁ 25–50% lower pressure

⦁ Novel chemistry

Chief Chromatographic Techniques Supported⦁ High performance liquid chromatography

⦁ Ultra high pressure liquid chromatography

⦁ Liquid chromatography–mass spectrometry

⦁ Reverse phase

⦁ Normal phase

⦁ Anion exchange

⦁ Cation exchange

⦁ Multi-mode

⦁ Small molecule separations

⦁ Protein separations

Markets Served All LC and LC–MS markets including: pharmaceutical, biotech,

contract research, forensic, clinical diagnostic, food and

beverage, industrial, chemical, environmental, and academic

research.

Imtakt USA

2892 NW Upshur St.

Portland, OR 97210

T E L E P H O N E

(215) 665-8902

F A X

(501) 646-3497

E - M A I L

[email protected]

W E B S I T E

www.imtaktusa.com


2004


Products include:

⦁ Pegasus BT® GC-TOFMS

The tried-and-tested reliability and

durability of our Pegasus brand in a

convenient benchtop unit

⦁ Pegasus 4D-C GC×GC-TOFMS

Four dimensions of analytical resolu-

tion offer more complete analysis of the

most complex samples

⦁ Pegasus GC-HRT+

GCMS with industry-leading resolution

(up to 50,000) and mass accuracy for

high-information content analysis

⦁ Pegasus GC-HRT+ 4D

Combining the highest performance

GCxGC and TOFMS on the market with

High Resolution Deconvolution®(HRD®)

Facility LECO’s global headquarters in St. Joseph,

Michigan include the Elizabeth S. Warren

Technical Centre, a 28,000 square-foot

facility exclusively dedicated to the research

and development of innovative instrumen-

tation and equipment for LECO’s separation

science line. Customer training demonstra-

tions and application support are available

at our Life Science and Chemical Analysis

Centre in St. Joseph. LECO has more than

25 subsidiaries worldwide, plus additional

international distributors.

LECO Corporation

Company Description For over 80 years, industries around the world have trusted

LECO to deliver technologically advanced products and

solutions for analytical science. Today’s technologies for

separation science answer key questions in complex samples

and pioneer high sample throughput using GCxGC, GCxGC-

TOFMS, GC-TOFMS, and high resolution GCxGC-TOFMS. All

instrumentation includes LECO’s exclusive ChromaTOF®

operating software for seamless data handling.

Chief Chromatographic Techniques Supported ⦁ GC-TOFMS

⦁ High Resolution GC-TOFMS

⦁ GCxGC-FID/ECD

⦁ GCxGC-TOFMS

⦁ High Resolution GCxGC-TOFMS

Markets Served LECO Separation Science instruments are used in a variety

of applications, helping users to uncover unidentified

compounds in their samples. Markets include metabolomics,

consumer product safety, food, flavor, fragrance,

environmental, and energy and fuels analysis.

Major Products/Services An ideal choice for laboratories investigating highly complex

samples, LECO products feature exclusive ChromaTOF brand

software with all the tools needed to easily locate, identify,

and quantify both target and unknown analytes, dramatically

reducing overall analysis times and increasing productivity.

LECO Corporation

3003 Lakeview Avenue

St. Joseph, MI 49085

T E L E P H O N E

(800) 292-6141

(269) 985-5496

F A X

(269) 982-8987

E - M A I L

[email protected]

W E B S I T E

www.leco.com


717


1936

Agilent Technologies is offering five years complimentary access to

CHROMacademy for all university students and staff.

CHROMacademy is an intuitive, comprehensive e-learning and trouble-

shooting platform with more than 3,000 pages of content for HPLC,

GC, sample preparation, and hyphenated techniques. No other online

resource offers separation scientists more live streaming events, a

knowledge base, practical solutions, and new technologies in one easy

to navigate website.

Get your free five year membership worth US $1,995* by submitting the

form at www.chromacademy.com/agilent.

* Five years free access to CHROMacademy only available to customers affiliated with an academic

or research institution, conditions apply. A valid university e-mail address if required.

© Agilent Technologies, Inc. 2017

CHROMACADEMY MEMBERSHIP

FREE


MACHEREY-NAGEL INC.

2850 Emrick Blvd.,

Bethlehem, PA 18020

T E L E P H O N E

(484) 821-0984

F A X

(484) 821-1272

E - M A I L

[email protected]

W E B S I T E

www.mn-net.com


ca. 620

(Worldwide)


1911

optimal and reliable solutions for analytical

task in method development and routine

analysis.

Major Products/Services NUCLEOSIL®, one of the first spherical silica

gels in the 1970s, is still packed in stainless

steel columns by MN. These columns with

various C18

modifications are reliable work-

horses and are validated in many drug test-

ing methods. In recent years new highly pure

and ultra-spherical HPLC and UHPLC silica

gels followed. Today, the product range of

totally porous NUCLEODUR® and fused-core

NUCLEOSHELL® columns comprise more

than 25 stationary phases, targeted to daily

routine analysis as well as for method devel-

opment in HPLC. More information:

www.mn-net.com/HPLC

MACHEREY-NAGEL, a pioneer in Thin-

Layer Chromatography, is still manufactur-

ing glass plates as well as aluminum and

plastic sheets coated with silica gels and

other sorbents for TLC and HPTLC. More

information: www.mn-net.com/TLC

Classical CHROMABOND® silica gel

phases with various modifications (such

as C18

, C8, NH

2, OH, CN) and modern poly-

meric resins are widely used for sample

preparation in environmental analysis, food

safety control, or clinical applications. The

hydrophilic-lipophilic balanced polymeric

SPE adsorbent CHROMABOND® HLB and

the CHROMABOND® HR-Xpert line of poly-

styrene-divinyl based RP and mixed-mode

ion-exchange resins offer a full toolbox of

innovative SPE products. More information:

www.mn-net.com/SPE

Facility The headquarters in Germany and

branches in the United States, Switzerland

and France, as well as a globally operating

network of distributors in more than 150

countries, ensure worldwide availability of

MN products and services.

MACHEREY-NAGEL GMBH & CO. KG

Company Description Since 1911, MACHEREY-NAGEL represents high quality, in-

novation, and reliability in analytical chemistry and life science.

Friendly expert advice and outstanding product quality have been

MN´s mission for over 100 years as a family-owned business in

the fourth generation. As one of today’s leading manufacturers,

MACHEREY-NAGEL offers a broad range of products for chroma-

tography, filtration, rapid tests, water analysis, and bioanalysis.

MN´s expertise in manufacturing of chromatography media

is based on many years of experience in silica technology. The

development of the spherical HPLC silica NUCLEOSIL® and the

introduction of commercially available ready-to-use TLC plates

and sheets are just two milestones emphasizing the pioneering

tasks of MN in promoting these separation techniques, which

today are omnipresent in analytical labs all over the world.

Chief Chromatographic Techniques Supported ⦁ SPE and Flash Chromatography

⦁ HPLC

⦁ TLC

⦁ GC

⦁ Chromatographic accessories (such as syringe filters, vials,

and caps)

Markets Served MN serves laboratories all over the world with HPLC, GC, and SPE

columns, TLC plates and sheets, syringe filters, or suitable vials

and caps. Customers from many different industries (such as the

food and beverage industry, pharmaceutical industry and health-

care, chemical industry, institutes for environmental analysis,

biotechnology, universities, and research institutes) benefit from


introduced many highly successful products

to the analytical laboratory:

⦁ UNITY-xr: A universal thermal desorption

unit for sorbent sampling tubes

⦁ TD100-xr: A fully automated TD system

for sorbent tubes

⦁ ULTRA-xr series of TD autosamplers

⦁ CIA Advantage-xr systems for auto-

mated cryogen-free analysis of air and

gas sampled using canisters

⦁ TT24-7xr for continuous air monitoring

⦁ The Micro-Chamber/Thermal Extractor

for fast sampling of emissions from

products and materials

⦁ Wide range of accessories and

consumables for TD

Markes also manufactures the BenchTOF

range of time-of-flight mass spectrometers

and associated software. Markes’

technological innovation for mass

spectrometry also includes Select-eV®

(ground-breaking ion-source technology)

and Tandem Ionisation®, for BenchTOF.

FacilitiesMarkes International’s factory, technical

center, and headquarters are located

near Cardiff, UK. The company also has

technical centers in Frankfurt, Germany,

Shanghai, China, and Gold River, California.

Markes also supports a global distributor

network. Markes is a company of the

Schauenburg International Group.

Markes International

Company DescriptionMarkes International, an industry leader in technology for trace

organic analysis, manufactures a range of instrumentation and

software that enhances the analytical capability and productivity

of GC–MS systems.

Chief Chromatographic Techniques Supported ⦁ Thermal desorption (TD) as a sample concentration and

introduction technique for GC

⦁ Time-of-flight mass spectrometry (TOF-MS) for GC and

GC×GC

Markets Served ⦁ Environmental

⦁ Consumer environmental health

⦁ Defense and homeland security and forensic

⦁ Food and beverage

⦁ Fragrance and odor profiling

⦁ Biological profiling

⦁ Petrochemical

Markes’ global customer base includes major industry,

government agencies, academia, and the contract service

laboratory sector.

Major Products/ServicesMarkes is globally recognized for its innovation, high-quality

products, unrivalled technical expertise, and high level of

customer service within the field of analytical TD. As a global

technology leader of thermal desorption for GC, Markes has

Markes International, Inc.

2355 Gold Meadow Way

Gold River, CA 95670

T E L E P H O N E

(866) 483-5684 (toll free)

Markes International Ltd.

Gwaun Elai Medi-Science

Campus, Llantrisant, RCT,

CF72 8XL, UK

T E L E P H O N E

+44 (0)1443 230 935

Markes International

GmbH

Schleussnerstrasse 42,

D-63263 Neu-Isenburg,

Frankfurt, Germany

T E L E P H O N E

+49 (0)6102 8825569

E - M A I L

[email protected]

W E B S I T E

www.markes.com

NUMBER OF EMPLOYEES: 110


1997


MicroSolv Technology Corporation

9158 Industrial Blvd. NE

Leland, NC 28451

T E L E P H O N E

(732) 380-8900

F A X

(910) 769-9435

E - M A I L

[email protected]

W E B S I T E

www.mtc-usa.com


Under 25


1992

MicroSolv Technology Corporation

Facilities Production facility and technical support

laboratory is located in upstate New

York and headquarters are in the greater

Wilmington, North Carolina area.

Company Description A privately held firm in the greater Wilmington, NC area, that

acquires, develops, and manufactures new technologies

and products for separation sciences. They serve pharma,

chemistry, medical and clinical R&D, environmental,

forensic, and biological laboratories worldwide through their

distribution network.


⦁ LC–MS

⦁ Capillary electrophoresis

Markets Served All analytical and biology laboratories that use HPLC, LC–MS,

GC, GC–MS, SFC, or CE in all markets segments and in all

countries around the world.

Major Products/Services Silica hydride based HPLC columns under the Cogent TYPE-C

Silica brand name, the RSA brand autosampler vials

(reduced surface activity), and the CElixir brand of dynamic

coatings for CE. Online community serves chromatographers

and provides a forum for sharing ideas and problem solving.

ES996842_LCGCSUPP1217_026.pgs 12.07.2017 17:22 ADV blackyellowmagentacyan

Discover the Diff erence…

» Detect low abundance analytes normally adsorbed by glass.

» Prevents pH changes in vials before injection even hours later.

» Prevents sample hydrolysis that can occur in your vials.

» No meniscus when water is used as the main solvent.

MANUFACTURED BY:

www.mtc-usa.com

Know What You

DIDN’T KNOW

» Polar and non-polar compounds can be separated in the same

isocratic run.

» Precision run to run, day to day, batch to batch.

» Equilibration time is much faster due to the diff erence in the

adsorption layer due to the weak attraction of the silica.

ANP

Replaces

HILIC

» Thermal Control

» Compatibility

» Orthogonality

» Sustainability

NEW

SAMPLE

MANAGEMENT

SYSTEM


Major Products/Services Our Milli-Q® Lab Water purification system

portfolio offers a broad range of pure and

ultrapure water purification systems and

services designed for scientists working

in both validated and non-validated

environments.

Facility The Life Science business of Merck KGaA,

Darmstadt, Germany, which operates as

MilliporeSigma in the U.S. and Canada,

has 60 manufacturing sites worldwide.

MilliporeSigma, the vibrant M and

Milli-Q are trademarks of Merck KGaA,

Darmstadt, Germany or its affiliates.

All other trademarks are the property

of their respective owners. Detailed

information on trademarks is available

via publicly accessible resources.

MilliporeSigma

Company Description The Life Science business of Merck KGaA, Darmstadt,

Germany, which operates as MilliporeSigma in the U.S. and

Canada, has 20,000 employees, with a portfolio of more than

300,000 products enabling scientific discovery. Udit Batra is

the global chief executive officer of MilliporeSigma.

Merck KGaA, Darmstadt, Germany completed its $17 billion

acquisition of Sigma-Aldrich in November 2015, creating a

leader in the $125 billion global life science industry.

Merck KGaA, Darmstadt, Germany is a leading company for

innovative and top-quality high-tech products.

Chief Chromatographic Techniques Supported ⦁ Analytical HPLC

⦁ Gas Chromatography

⦁ Ion Chromatography

⦁ TLC and HPTLC

⦁ Mobile Phases for Chromatography


⦁ Clinical

⦁ Academic

⦁ Industrial

⦁ Research

⦁ Government Laboratories

MilliporeSigma

400 Summit Drive,

Burlington, MA 01803

T E L E P H O N E

+1 (800) 645-5476

E - M A I L

[email protected]

W E B S I T E

www.emdmillipore.com


20,000


1668


Mott Corporation

84 Spring Lane

Farmington, CT 06032

T E L E P H O N E

(860) 747-6333

F A X

(860) 747-6739

E - M A I L

[email protected]

W E B S I T E

www.mottcorp.com


1959

Mott Corporation

Major Products/Services Mott specializes in the use of porous

metal and 3D printing for the following

fluid control products: industrial filters,

high purity filters, solvent sinker filters,

inline filters, static mixers, column frits,

flow restrictors, industrial spargers, micro-

spargers, and various other filtration and

flow control assemblies. Each solution is

customizable to meet the specifications

of your system. In addition, Mott offers

the following engineering services: rapid

prototyping, world class product design

consultation, and state-of-the-art lab

testing and support.

Facility Mott’s headquarters and manufacturing

facility operate in two adjacent facilities in

Farmington, Connecticut. Manufacturing

facility includes both a Class 100 and Class

10,000 cleanroom for applications with

high cleanliness requirements. Mott has

remote offices in various countries and a

global distribution network.

Company Description Mott is a high precision filtration and flow control company

trusted by the world’s best known technical and performance

brands. Often, our customers have no effective options to

solve their challenging problems and turn to us for solutions.

Our strategic vision is to deliver technological breakthroughs

where none currently exist.


⦁ UHPLC

⦁ LC–MS

⦁ GC

Markets Served Mott provides various product solutions and design

consultation support to the following industries: Analytical

instrumentation, pharmaceutical, biotechnology, food &

beverage, medical, microelectronics, chemical processing,

aerospace, environmental, process control, and energy.


We also offer an integrated program called

Laboratory Equipment Asset Procurement

Services (LEAPS) that provides new and

existing customers within the global

science market with convenient access

to end-to-end laboratory services and

insights. It combines OEM contract

administration, parts and relocation

services, and reporting with tier 1

diversity spend and cost savings to drive

operational efficiencies and economic

outcomes nationwide.

Markets Served Neta Scientific serves research and

development labs throughout North

America, encompassing the pharmaceutical,

biotech, life sciences, academia and research

institutions, government facilities, food and

flavors, industrial testing, and chemical

industries.

Facility The corporate offices and main warehouse

are headquartered in Hainesport, New

Jersey. We also maintain additional

representation throughout the United States.

Neta Scientific Inc.

Company Description Neta Scientific is a laboratory supplies and solutions company

that enables the global science and clinical market to more

effectively conduct world-leading research and development.

We centralize superior supplies, services, and supply chain

management with a customer-centric approach, innovative

technologies and diversity certifications for customers to easily

gain access to the tools needed to improve global health and

safety and meet corporate objectives.

Major Products/Services At Neta Scientific, we enable your mission by centralizing

superior laboratory supplies and supply chain management

with our unique ability to provide pass-through support

for major brand suppliers, as well as OEM contract, parts,

consumables, and services to give you back the time you

need.

⦁ Chromatography

⦁ Life Sciences

⦁ Labware

⦁ Chemicals & Reagents

⦁ Services (LEAPS)

⦁ Small & Large Bench Instruments

⦁ Cold Storage

Neta Scientific Inc.

4206 Sylon Blvd.,

Hainesport, NJ 08036

T E L E P H O N E

(800) 343-6015

F A X

(609) 265-8213

E - M A I L

[email protected]

W E B S I T E

www.netascientific.com


40+


1999


Parker Hannifin

Corporation

Gas Separation and

Filtration Division

4087 Walden Avenue

Lancaster, NY 14086

T E L E P H O N E

(978) 858-0505

F A X

(978) 556-7501

W E B S I T E

www.labgasgenerators.com


55,000


1924

Parker Hannifin Chief Chromatographic Techniques Supported ⦁ GC–FID, FPD, NPD

⦁ GC–HALL, ECD, TCD, HST-Wire

⦁ Capillary separations

⦁ Packed separations

Markets Served ⦁ Agriculture

⦁ Biotechnology

⦁ Chemicals

⦁ Chemical and explosives detection

⦁ Energy

⦁ Environmental

⦁ Inorganic chemicals

⦁ Instrument development

⦁ Life science

⦁ Organic chemicals

⦁ Paints and coatings

⦁ Petrochemicals

⦁ Pharmaceuticals

⦁ Plastics

Major Products/Services Gas generators for the following analytical

instruments:

⦁ Gas chromatographs

⦁ LC–MS

⦁ FT-IR spectrometers

⦁ ICP emission spectrometers

⦁ TOC analyzers

⦁ Atomic absorption spectrophotometers

⦁ Nuclear magnetic resonance (NMR)

⦁ Rheometers/thermal analyzers

⦁ Sample evaporators/concentrators

Facilities Parker Hannifin manufactures all gas genera-

tors in Haverhill, Massachusetts. Parker has

over 50,000 generators installed globally and

distribution points worldwide.

Company Description Safety: Parker Balston gas generators completely eliminate the

safety hazards involved with handling high-pressure gas cylinders.

Enjoy hassle-free automation with no tanks to charge and no

downtime.

Reliability: Thousands of laboratories worldwide have

Parker Balston gas generators in routine use. Parker Balston

gas generators are recommended and used by major instru-

ment manufacturers. We offer the best technology at an af-

fordable price from the brand you trust.

Quality: Each Parker Balston gas generator is manufactured

under a strict total quality management program. We have a

world-class ISO 9001-certified manufacturing facility in the

United States. All Parker Balston gas generators are backed

by a complete satisfaction guarantee. Parker offers preventa-

tive maintenance, extended warranties, and field repair pro-

grams for all laboratory gas generators, as well as a network

of highly specialized sales, application, and technical support

people.

Products: Hydrogen gas generators produce 99.99999%

pure hydrogen for gas chromatographs. Zero air genera-

tors produce zero grade air for gas chromatographs. UHP

nitrogen generators produce 99.9999% pure nitrogen for

GCs or ICP spectrometers. FT-IR gas generators produce

dry, CO2-free purge gas for FT-IR spectrometers. Pure

air and nitrogen generators produce dry, ultrapure com-

pressed gas for laboratory instruments, including LC–MS

instruments.


Major Products/Services PerkinElmer, Inc. offers a wide range of

instrumentation and solutions to meet

your analytical measurement needs:

⦁ Atomic spectroscopy: AA, ICP-OES,

ICP-MS

⦁ Chromatography: GC and GC custom

solutions, GC–MS, HPLC and UHPLC,

LC–MS

⦁ Hyphenated techniques: HPLC–ICP-MS,

GC–ICP-MS, HS-GC, HS-GC-MS, TD-GC,

TD-GC–MS, TG-IR, TG-MS, TG-GC–MS

⦁ Mass spectrometry: ICP-MS, GC–MS,

LC–MS

⦁ Molecular spectroscopy: FT-IR and

FT-NIR, UV-vis and UV-vis-NIR,

fluorescence spectroscopy

⦁ Thermal analysis: DSC, TGA, STA, DMA

⦁ Organic elemental analysis: CHN/O,

CHNS/O

⦁ Consumables: atomic spectroscopy,

chromatography, mass spectrometry,

molecular spectroscopy, thermal

analysis, elemental analysis

⦁ OneSource® Laboratory Services

Facility PerkinElmer, Inc. operates globally in 150

countries.

PerkinElmer, Inc.

Company Description PerkinElmer is a global leader focused on improving human

and environmental health, for the better. We provide our

customers with critical knowledge, expertise, and innovative

detection, imaging, software, and services so that they can

make better decisions for better outcomes. At PerkinElmer,

we make a difference every day helping scientists, clinicians,

and governments detect earlier and more accurately to

improve the health and safety of people and the environment.

Our solutions range from enabling the discovery of more

effective diagnostics and therapies, to making sure that the

food we eat, the water we drink, and our environment are

safe from contaminants.

Chief Chromatographic Techniques Supported⦁ Gas chromatography

⦁ GC–MS and portable GC–MS

⦁ Liquid chromatography—HPLC and UHPLC

⦁ Headspace and thermal desorption sample handling

⦁ LC–MS

Markets Served PerkinElmer is a leading provider of precision instrumenta-

tion, reagents and chemistries, software, and services for a

wide range of scientific and industrial laboratory applications,

including environmental monitoring, food and beverage qual-

ity/safety, and chemical analysis, as well as genetic screening,

drug discovery, and development.

PerkinElmer, Inc.

940 Winter Street

Waltham, MA 02451

T E L E P H O N E

(781) 663-6900

E - M A I L

CustomerCareUS@

perkinelmer.com

W E B S I T E

www.perkinelmer.com


7700


1937


Markets Served Mass spectrometry, nuclear detection,

medical instrumentation, pharmaceutical

safety, industrial instrumentation, and image

intensification, as well as the custom design

and manufacture of detectors, sensors, and

ion mobility analyzers.

Major Products/Services PHOTONIS offers a complete range of

high performance scientific and medical

detector products. Our market includes

electron multipliers, microchannel plates,

mass spectrometry fiber optics and resistive

glass, advance performance time-of-flight

detectors, image intensifiers, ion mobility

analyzers, neutron imagers, and other related

products. Our detection products are found

in most of today’s technology-based markets,

including analytical instrumentation, medical

diagnostics, chemistry, drug discovery,

high-energy physics, space exploration, and

scientific research. PHOTONIS is the largest

supplier of standard, retrofit, and custom

detectors in the mass spectrometry, residual

gas analyzer, and electron microscope

markets providing advanced detector designs

for the highest sensitivity through superior

signal collection and noise reduction.

Facilities Sturbridge, MA; Lancaster, PA; Frisco, TX;

Brive, France; Roden, Netherlands.

PHOTONIS USA

Company Description PHOTONIS is a multinational high-technology group, with

more than 40 years of experience in manufacturing, sales, and

innovation, specializing in charged particle and photon sensor

technology. The group operates internationally in the night vision,

industrial, scientific, and medical markets.

Chief Chromatographic Techniques Supported ⦁ Liquid chromatography detectors

⦁ Ion mobility spectrometry

⦁ Mass spectrometers

⦁ UV–vis fixed wavelength

⦁ UV–vis variable wavelength

⦁ Gas chromatography detectors and accessories

⦁ Detector accessories

⦁ Mass spectrometers: high-resolution

⦁ Mass spectrometers: low-resolution

⦁ Mass spectrometers, residual gas analysis, magnetic

⦁ Mass spectrometers, residual gas analysis, quadrupole

⦁ ICP-MS

⦁ Time-of-flight mass spectrometry

⦁ Other detectors and accessories

⦁ Supercritical fluid chromatography detectors and accessories

⦁ Radioactivity

⦁ Neutron imaging

PHOTONIS USA

660 Main Street

Sturbridge Business Park

Sturbridge, MA 01566

T E L E P H O N E

(508) 347-4000

F A X

(508) 347-3849

E - M A I L

[email protected]

W E B S I T E

www.photonis.com


US: 150

Elsewhere: 900


1937


⦁ Medical analysis

⦁ Pharmaceutical analysis

Major Products/Services Reference Materials

⦁ GPC/SEC standards and kits

⦁ Certified reference materials

⦁ MALDI kits

⦁ Viscosity and light scattering validation kits

⦁ ReadyCal kits

⦁ Deuterated polymers

⦁ Tailor-made polymers

High Resolution Columns

⦁ GPC/SEC columns for all organic

eluents and all aqueous eluents

⦁ For high and low molecular weight

synthetic and biopolymers

⦁ For micro GPC/SEC up to preparative

scale

⦁ HighSpeed columns for fast analysis

Instrumentation and Software

⦁ GPC/SEC systems and components

⦁ Light scattering detectors

⦁ Viscosity detectors

⦁ dn/dc instrumentation

⦁ WinGPC UniChrom software

⦁ PoroCheck software for inverse GPC/SEC

Contract analysis (including HT-GPC

and specialty solvents)

⦁ Contract analysis

⦁ Molar mass determination

⦁ Branching/structure information

⦁ Method development and transfer

⦁ Complete product deformulation

⦁ Consulting

GPC/SEC Academy and Support

⦁ Full services from installation to valida-

tion, operation, and repair

⦁ GPC/SEC and software training schools

⦁ GPC/SEC in-house training

⦁ User meetings

⦁ NetCommunity with application and pub-

lication downloads

Facility PSS US office is located in Amherst, MA.

Headquarters are located in Mainz, Germany.

PSS also has offices in The Netherlands and

works with a network of distributor partners

in most other countries around the world.

PSS Polymer Standards Service Inc.

Company Description GPC/SEC/GFC is a versatile technique for characterizing polymers,

biopolymers, polysaccharides, and proteins used to determine

molecular weight, size, and architecture. PSS is fully dedicated to

the advancement of macromolecular liquid chromatography by

developing true solutions and providing competent and personal

support. Based on excellent products and cutting-edge technol-

ogy, PSS creates easy-to-use and powerful solutions for QC and

R&D. From a single molar mass reference material, contract

analysis, and consulting, to turn-key systems for GPC/SEC multi-

detection with light scattering, viscometry, mass spectrometry, or

fully compliant GPC/SEC for the pharmaceutical industry: PSS of-

fers products and services required for successful macromolecular

analysis with expert support by GPC/SEC enthusiasts.

Chief Chromatographic Techniques Supported ⦁ Size-exclusion chromatography (SEC)

⦁ Gel permeation chromatography (GPC)

⦁ Gel filtration chromatography (GFC)

⦁ High-Temperature GPC (HT-GPC)

⦁ GPC/SEC viscometry / light scattering / triple

⦁ GPC/SEC mass spectrometry

⦁ Two-dimensional chromatography

Markets Served Fully featured PSS applications provide solutions for the

analysis of natural and synthetic macromolecules divided into

the following sections:

⦁ Biopharma

⦁ Chemical manufacturing

⦁ Food analysis

PSS Polymer Standards

Service Inc.

160 Old Farm Road,

Amherst, MA 01002

T E L E P H O N E

(413) 835-0265

E - M A I L

[email protected]

W E B S I T E

www.pss-polymer.com


1994


Molex

18019 N 25th Ave.,

Phoenix, AZ 85023

T E L E P H O N E

(602) 375-4100

F A X

(630) 813-9995

W E B S I T E

www.molex.com/polymicro

Molex cal sciences, in addition to pharmaceutical,

environmental, and petrochemical analysis,

genomics, proteomics, neurology, clinical in-

strumentation, and a wide variety of related

technologies. Typical applications include re-

search, process monitoring and control, qual-

ity monitoring and control, forensics, package

leak testing, drug delivery, and DNA fragment

analysis (HID), in addition to unique applica-

tions requiring precision drawn capillary.

Major Products/Services Advances in chromatography research and

analysis have been aided by proprietary

improvements to the properties of Molex

synthetic fused capillary tubing and

techniques for handling, cutting, cleaving,

and laser machining. Molex manufactures

Polymicro high-strength, high-temperature,

flexible fused silica capillary tubing; square

capillary tubing; windowed capillary tubes;

UV transparent coating; precision cleaved-

to-length tubing; multi-lumen tubing;

multimode, step-index fused silica optical

fiber with polyimide, silicone, acrylate and

fluoropolymer buffers; solarization resistant

optical fiber for deep UV; broad spectrum

fiber; IR waveguides; fiber optic cables and

assemblies; silica and quartz base materials;

and micro-components including laser

machined fiber tips, telecommunications

ferrules and sleeves and Inner Lok™ unions

and Y-splitters for GC. New innovations

include VS Tight-Tolerance capillary, nano-

Capillary, and fiber bundles.

Facility Polymicro products are manufactured at

Molex’s facility located in Phoenix, Arizona.

The state-of-the art facility is ISO 9001,

13485, and 14001 certified and features

a glass laboratory, multiple draw towers,

sophisticated testing and laser machining

capabilities. We offer large and custom

orders, in addition to select, pre-packaged

Polymicro capillary tubing and optical fibers

for smaller orders. Please contact us at

[email protected] to initiate a

quote or request a copy of the Polymicro

solution handbook.

Company Description As a global leader in capillary tubing and specialty optical fiber

products for chromatography, Molex designs and manufactures

a large portion of Polymicro capillary tubing and multimode

step-index fibers used throughout the world. The company’s

extensive portfolio of products serves applications such as

aerospace, military and defense, analytical, astronomy, automo-

tive, biotechnology, communications, energy, manufacturing,

medical, pharmaceutical, and telecommunications.

Molex solves complex optical challenges for the world’s innova-

tors by anticipating and delivering what our customers need most.

From a globally integrated approach to seamless collaboration, we

bring expertise and innovation together to help bring your product

vision to reality. Our team works closely with yours to deliver the

results for your specific application needs by leveraging our heri-

tage of innovation, industry expertise and superior quality.

Chief Chromatographic Techniques Supported ⦁ Capillary Electrophoresis, CE

⦁ Cytometry

⦁ Gas Chromatography, GC

⦁ High Pressure Liquid Chromatography, HPLC

⦁ Lab-on-a-chip

⦁ Liquid Chromatography, LC

⦁ nano-Liquid Chromatography, nano-LC

⦁ Spectroscopy, Ultra Violet to Mid-Range Infrared

Markets ServedMolex capillary tubing, optical fibers, fiber optic assemblies, fiber

and tubing arrays, and bundles are commonly used in the analyti-


Facilities EMEAI

Postnova Analytics GmbH

Max-Planck-Strasse 14

86899 Landsberg, Germany

Tel: +49 8191 985 688-0

Fax: +49 8191 985 688-99

[email protected]

UNITED KINGDOM

Postnova Analytics UK Ltd.

Unit 64, Malvern Hills Science Park,

Malvern, Worcestershire

WR14 3SZ, UK

Tel: +44 1684 585167

[email protected]

NORTH AMERICA

Postnova Analytics Inc.

230 South, 500 East, Suite 110

Salt Lake City, UT 84102, USA

Tel: +1 801 521-2004

Fax: +1 801 521-2884

[email protected]


Company Description Postnova Analytics offers the worldwide unique FFF-Platform,

which is a range of different field-flow fractionation (FFF)

and light scattering systems (MALS) for advanced separation,

fractionation, and characterization of nanoparticles, proteins,

polymers, and bio-macromolecules. Postnova offers a truly

complete FFF-MALS product range, which is widely used for

applications in biopharmaceutical, nanotechnology, environ-

mental, food, cosmetics, and polymer science. The Postnova

FFF-Platform can also be ideally coupled to dynamic light scat-

tering (Malvern Zetasizer Nano DLS) and inductively coupled

plasma–mass spectrometry (Agilent Technologies ICP-MS),

which provides high resolution size and molar mass separa-

tions as well as characterization and element speciation.

Chief Chromatographic Techniques Supported⦁ Field-flow fractionation (FFF)

⦁ Asymmetric FFF

⦁ Thermal FFF

⦁ Centrifugal FFF

⦁ Multi-angle light scattering (MALS)


230 South, 500 East, Suite 110

Salt Lake City, UT 84102

T E L E P H O N E

+1 (801) 521-2004

F A X

+1 (801) 521-2884

E - M A I L

[email protected]

W E B S I T E

www.postnova.com


Major Products/Services Proton OnSite offers hydrogen, nitrogen,

and zero air gas generators to fit the

needs of any laboratory. These units are

suited to provide ultra-high purity gas with

consistent and predictable results. Also,

Proton OnSite is the only provider of “Lab

Server” solutions, allowing large labs to

supply numerous mass spec instruments

from a single, central generator. Our line

of on-site gas generators is a simpler, less

expensive, and less complex approach to

supplying superior gas for laboratory and

scientific applications. These generators

are compact in size and available in

stackable configurations to maximize your

available lab space.

Proton OnSite

Company Description Proton OnSite, a wholly owned subsidiary of Nel ASA, Oslo,

Norway (OSE:NEL) manufactures hydrogen, nitrogen, and

zero air generators that you can rely on. Our advanced proton

exchange membrane (PEM) electrolysis systems, coupled

with uncompromising attention to excellence and quality,

enable us to deliver, install, and support gas generation

on every continent. No other company has as large of a

commercial fleet or as long of a history with reliability, as

Proton OnSite. Exclusively focused on customer solutions, we

have the experience and products to ensure success.

Markets Served ⦁ Environmental sciences

⦁ Food and beverage industries

⦁ Life sciences

⦁ Petrochemical industry

⦁ Pharmaceutical industry

Proton OnSite

10 Technology Dr.

Wallingford, CT 06492

T E L E P H O N E

(203) 678-2300

F A X

(203) 949-8016

E - M A I L

[email protected]

W E B S I T E

www.protononsite.com


100


1996


Major Products/Services GC, GC–MS, LC, LC–MS, Sample Prepara-

tion, Sample Introduction, SIFT-MS, Min-

iaturized Mass Detector, Trace Elemental

Analysis, Molecular Spectroscopy, NMR

Spectroscopy, Metrology, Digital Holo-

graphic Microscopy, Gas Generators

Facility Quantum Analytics is headquartered in

Foster City, California and has a Tech Cen-

ter in North Houston, Texas.

Quantum Analytics

Company Description Quantum Analytics is a value-added distributor for analytical

instrumentation across the United States. We offer technical

application services, a range of laboratory analytical instruments,

and flexible financing programs. We make it easier for customers

to get the lab equipment they need through flexible financing

solutions and customized technical support. We maintain a

multi-million dollar inventory of new and refurbished instruments

available for immediate delivery. Our technical services

include cross-platform system integration, installation, training,

application development and product support.

Chief Chromatographic Techniques SupportedGas chromatography, liquid chromatography, ion chromatog-

raphy, mass spectrometry, selected ion flow tube mass spec-

trometry, real-time gas analysis, miniature mass spectrometry

Markets Served Academia, automotive, biopharma, cannabis, chemicals, clini-

cal, defense, electronics, energy, environmental, food and

flavors, forensics and drugs, government, industrial, MEMS,

petrochemical, polymers, semiconductor, and more.

Quantum Analytics

3400 East Third Avenue,

Foster City, CA 94404

T E L E P H O N E

(650) 312-0900

F A X

(650) 312-0303

E - M A I L

[email protected]

W E B S I T E

www.LQA.com


1989


⦁ Industrial hygiene

⦁ Petrochemical

⦁ Pharmaceutical

Major Products/Services Plus 1 Service in everything we do. Living this

corporate core value every day ensures we

will surpass your expectations every time you

contact us! Our customer service team will

suggest time- and money-saving options and

is dedicated to getting your products to you

fast. Our technical service chemists can help

you from set-up to method development.

Visit our website where you can blog with

our chemists and review an extensive library

of technical publications, chromatograms,

product documentation, step-by-step guides,

interactive calculators, animations, and

educational material.

Restek’s commitment to continuous

innovation in chromatography sets us apart

from our competitors. We introduce and

stock hundreds of new products every year,

designed by chromatographers for

chromatographers.

⦁ Exceptional columns for UHPLC, HPLC,

LC–MS, GC, GC–MS, and GC×GC

⦁ Innovative accessories, instrument

replacement parts, and consumables

⦁ Air monitoring canisters and sampling

supplies

⦁ Sample prep products

⦁ Reference standards: stock and

custom-prepared formulations

⦁ Thousands of innovative products,

hundreds of chromatograms

Facilities Restek opened for business in 1985 in

a small business incubator in central

Pennsylvania. Today, more than 350

employee-owners work, play, and

celebrate milestones in a state-of-the-art

140,000-square-foot facility in Pennsylvania,

in our research facility in California, and in

our subsidiary locations in China, England,

France, Germany, Italy, and Japan.

Restek Corporation

Company DescriptionFor over 30 years, Restek has been a leader in developing

technologies and manufacturing products for gas and liquid

chromatography (GC and LC), including columns, reference

standards, sample preparation materials, accessories, and

more. We have decades of hands-on, practical experience

in chemistry, chromatography, and engineering, and our

reputation for going the extra mile with Plus 1 customer

service and top-performing products is well known

throughout the chromatography community. Restek is proud

to assist analysts around the world with monitoring the

quality and safety of air, water, soil, food, pharmaceuticals,

and petroleum. We proactively offer integrated solutions—

products, applications, and assistance—perfectly matched to

your needs, regardless of your industry. www.restek.com

Chief Chromatographic Techniques Supported ⦁ UHPLC

⦁ HPLC

⦁ LC–MS

⦁ GC

⦁ GC–MS

⦁ GC×GC

Markets Served ⦁ Air monitoring

⦁ Chemical

⦁ Clinical

⦁ Environmental

⦁ Food safety

⦁ Forensic

Restek Corporation

110 Benner Circle

Bellefonte, PA 16823

T E L E P H O N E

(800) 356-1688

F A X

(814) 353-1309

E - M A I L

[email protected]

W E B S I T E

www.restek.com


350


1985


Sciencix

14261 W. Burnsville Pkwy.

Burnsville, MN 55306

T E L E P H O N E

(800) 682-6480

F A X

(952) 895-8493

E - M A I L

[email protected]

W E B S I T E

www.sciencix.com


USA: 33


1985

Sciencix

Company Description Sciencix has been a trusted provider of top brand HPLC

instrumentation products for more than 30 years. Our

products are equivalent to OEM products and can help reduce

repair expenses by 15% to 30%. Our mission is to serve our

customers by delivering quality service and quality products

at a reasonable price while exceeding expectations, every

moment.

Chief Chromatographic Techniques SupportedAny technique used in or related to high performance liquid

chromatography (HPLC) replacement parts.


⦁ HPLC service companies

⦁ Academia

⦁ Biotechnology

⦁ Testing facilities

Major Products/Services Sciencix provides alternative repair parts to OEMs such as

Agilent®, Waters®, Shimadzu®, and many more. We offer

replacement parts for autosamplers, pumps, detector lamps,

filters, fittings, and tubing.

Facility Sciencix is headquartered in Burnsville, Minnesota. Our facility

includes manufacturing, R&D, and quality departments. We

are able to ship products world-wide.


Facility Showa Denko America, Inc. based in New

York, New York provides technical and

sales support for Shodex HPLC columns

for customers and distributors in North

and South America. Our stock point in

New York allows for fast delivery in the

United States. Working with our laboratory

in Japan, we support customers’ analytical

needs.

TM

Shodex/Showa Denko America, Inc.

Company Description Shodex HPLC columns have been manufactured by Showa

Denko K.K. in Japan since 1973. Showa Denko K.K. is one of

Japan’s largest chemical companies. Our strength in polymer

synthesis led to the production of many unique and durable

polymer-based columns. The golden-mechanical fish is a

symbol of fast and smooth liquid chromatography analysis.

Chief Chromatographic Techniques SupportedHPLC column selection, method development, and trouble

shooting, as well as providing educational seminars

Markets Served ⦁ North America

⦁ South America

⦁ HPLC

⦁ Size exclusion

Major Products/Services Shodex carries thousands of different HPLC columns with

various separation modes. We are best known for high quality

size exclusion and saccharide analysis columns, as well as

unique polymer-based reversed and HILIC phase columns.

With additional selections of normal phase, ion exchange, ion

chromatography, high temperature, and multimode columns,

we are confident we can provide the most suitable column

for customers’ needs.

Shodex/Showa Denko

America, Inc.

420 Lexington Ave.

Suite #2335A

New York, NY 10170

T E L E P H O N E

(212) 370-0033

F A X

(212) 370-4566

E - M A I L

[email protected]

W E B S I T E

www.shodexhplc.com


1973


Shimadzu Scientific

Instruments

7102 Riverwood Drive

Columbia, MD 21046

T E L E P H O N E

(800) 477-1227

(410) 381-1227

F A X

(410) 381-1222

E - M A I L

[email protected]

W E B S I T E

www.ssi.shimadzu.com


US: 440

Worldwide: 11,500


Shimadzu Scientific

Instruments: 1975

Shimadzu Corporation: 1875

Shimadzu Scientific Instruments

Major Products/Services Shimadzu offers a wide range of advanced

UHPLC/HPLC, GC, and mass spectrometry

systems and components. Key systems

include:

⦁ Nexera X2 UHPLC—At 19,000 psi, the

modular Nexera achieves world-leading

sensitivity and superior separation

across the entire HPLC/UHPLC range.

⦁ Prominence UFLC/HPLC—Raises total

system productivity to new levels by

generating high-quality, reproducible

data without sacrificing performance,

reliability, or ruggedness.

⦁ i-Series Plus Integrated HPLC/UHPLC—

Providing the ultimate balance of high-

level performance and ease-of-use in

an integrated HPLC.

⦁ Nexera UC SFE-SFC—First fully auto-

mated system that combines on-line

SFE and SFC in a single flow path

⦁ LCMS-2020/8040/8045/8050/8060—

User-friendly single-quad and triple quad

LC–MS-MS systems deliver outstanding

speed, sensitivity, and reliability.

⦁ Nexis GC-2030—Offers a modern ap-

proach to a classic chromatographic

technique, delivering exceptional perfor-

mance and faster ROI.

⦁ System GC—Fully applicated GC systems

based on industry-standard methods.

⦁ GCMS-QP2020/SE/TQ8040/TQ8050—

With Smart Technologies, single-quad

and triple-quad GC–MS/MS systems

enable new possibilities in sensitivity,

durability, performance, stability, and

reliability.

Facilities Shimadzu’s US headquarters includes a cus-

tomer service and training center, a solution

center to showcase technologies, and an

innovation center for promoting collaborative

projects with customers. Shimadzu’s regional

facilities, strategically located around the US,

provide customers with local sales, service,

and technical support.

Company Description Shimadzu Scientific Instruments (SSI) is the North American

subsidiary of Shimadzu Corp., headquartered in Kyoto, Japan.

SSI was established in 1975 to provide analytical solutions

to a wide range of laboratories in the Americas. With a

vast installed base and preferred vendor status at many

institutions, SSI’s instruments are used by top researchers

across the globe, customers who can count on the stability,

experience, and support only Shimadzu offers.

Chief Chromatographic Techniques Supported ⦁ Ultra high-pressure LC

⦁ Ultra-fast LC

⦁ Analytical/prep HPLC

⦁ 2D LC

⦁ Online SFE-SFC

⦁ LC–MS/MS

⦁ GC

⦁ Comprehensive LC and GC

⦁ GC–MS/MS

Markets Served Shimadzu’s product line flexibility enables chromatographers

in any environment to select the instrument best suited to their

application. Shimadzu instruments are found in a wide range of

labs, including academic research, pharmaceutical, forensic, food

and beverages, petrochemical, life sciences, and environmental.

Shimadzu provides free technical support for the life of the

instruments and encourages customer alliances to further

product development.

Order consumables and accessories on-line at http://store.shimadzu.com Shimadzu Scientific Instruments Inc., 7102 Riverwood Dr., Columbia, MD 21046, USA

The Next Industry StandardGas Chromatograph

Nexis GC-2030, Shimadzu’s premier gas chromatograph, offers a modern approach to a classic

chromatographic technique. Designed with the user in mind, new innovative features, exceptional

performance and high-throughput capabilities will elevate your lab to the next level.

X Faster ROI with versatile, expandable configurations

X Reduced operating costs with Eco mode

X Improved workflow with touch-panel interface and remote communications

X Increased productivity with tool-free column installation and inlet maintenance

X Simplified operation with enhanced data browser and Quick-Batch function

Learn more about Shimadzu’s GC-2030. Visit us online at www.ssi.shimadzu.com/GC2030


⦁ Process

⦁ Analytical

⦁ Bio/Pharma

⦁ Oil & Gas

⦁ Refining

⦁ Petrochemical

⦁ Semiconductor

⦁ Corrosion

⦁ Coking/Fouling

⦁ Automotive

⦁ Aerospace

Major Products/Services SilcoTek® offers custom silicon coatings

to OEM and labs worldwide. Send in your

stainless steel, alloy, glass, or ceramic part

and we’ll apply our coatings in 10 days or

less. Need a coated part sooner? Many GC

and LC component suppliers offer coated

products from stock. Our coatings:

⦁ SilcoNert®: The most inert coating

available.

⦁ Dursan®: An inert, high durability

coating. Ideal for lab and field analysis.

⦁ Silcolloy®: A high purity corrosion

resistant coating.

⦁ SilcoKlean®: Prevent carbon coking and

fouling in automotive and aerospace.

⦁ SilcoGuard®: A low outgassing, high

purity coating, ideal for research and

semiconductor applications.

Facility SilcoTek® applies coatings to customer-

supplied products from their 35,000

square foot state-of-the-art facility located

in Bellefonte, Pennsylvania. Want to speak

to an expert about your application? Email

[email protected] or call + (814)

353-1778. To find a sales office near you,

please visit

www.silcotek.com/international-reps.

SilcoTek® Corporation

Company Description SilcoTek® Corporation is the world’s leading provider of flow

path deactivations and coatings. Our patented CVD coating

technologies improve analytical reliability, increase system

uptime, and boost your bottom line. SilcoTek’s coatings are used

in a large, diverse variety of industries and applications where

analytical accuracy and superior performance are critical. Whether

for industry-leading chemical compatibility, corrosion resistance,

bio-inertness, or hydrophobicity, SilcoTek coatings will expand the

material limits of your instruments and products.

Chief Chromatographic Techniques Supported⦁ Sulfur analysis

⦁ VOC analysis

⦁ Environmental

⦁ Thermal desorption

⦁ Fenceline monitoring

⦁ CEMS

⦁ Flare subpart JA

⦁ Stack monitoring

⦁ Protein analysis

⦁ NOx, SOx testing

⦁ GC

⦁ LC

Markets Served SilcoTek® coating technologies are utilized in diverse applications

and industry segments world wide. Since 1987, customers have

relied on SilcoTek® to improve the performance of their products

and increase revenue in the following markets and applications:

SilcoTek Corporation

225 PennTech Drive,

Bellefonte, PA 16823

T E L E P H O N E

(814) 353-1778

F A X

(814) 353-1697

E - M A I L

[email protected]

W E B S I T E

www.SilcoTek.com


www.SilcoTek.com


2009


Sorbent Technologies, Inc.

5955 Peachtree Corners East

Norcross, GA 30071

T E L E P H O N E

(770) 936-0323

Toll-free (866) 767-2832

F A X

(770) 936-0326

E - M A I L

[email protected]

www.sorbtech.com


2000

Sorbent Technologies, Inc. Markets Served Sorbtech has deep expertise in

chromatography and purification

research, and develops lasting, strong

relationships with the laboratories we

serve. Our broad product offering ensures

success—from simple lab procedures to

complex chromatographic techniques. The

industries we supply include academic,

bioprocess, chemical, food/beverage,

forensic, pharmaceutical, power, and

water. We assist from R&D method

development to process manufacturing:

CROs, academia (organic, bio-chemistry,

bioprocess, polymer/material science),

and government facilities (US FDA, NIH,

USDA, USGS, DOE).

Major Products/Services Sorbtech is one of the premier

chromatography suppliers, offering the

products, information and consultation you

need to meet lab and project requirements.

We provide comprehensive selection of

unique goods and services that deliver

value, including a free applications

database, high-end life science products,

and a customer referral program. To ease

the sales process, a personal product

specialist is assigned to each lab to

assist with method development and

optimization. This helps shift the burden of

purchasing supplies from your shoulders

to Sorbtech’s, allowing your lab to focus on

what matters most—achieving innovative,

successful outcomes.

Facility Sorbtech is located in Norcross, Georgia

within the Innovation Crescent—a

geographic area of economic development

entities dedicated to Georgia’s life science

and technology growth. Our facility includes

a state-of-the-art research and development

lab, production areas, and an efficient

warehouse that stores our vast inventory of

chromatography and purification products.

Company Description Sorbent Technologies, Inc. understands that chromatography

is the catalyst that drives success after weeks, or even years

of hard work. We partner with our customers to provide

the most effective solutions for their projects’ unique

challenges, helping meet stringent lab deadlines and budget

requirements, while protecting research integrity. In addition

to a large selection of chromatography products, Sorbtech

offers user-friendly laboratory equipment and supplies to

optimize performance, ensure lab safety, and create efficiency.

We select products based on performance and reliability

to help grant or investment dollars go further, delivering

confidence for lab, pilot, and industrial process applications.

Chief Chromatographic Techniques Supported⦁ Column chromatography (Flash, MPLC, and Gravity): bulk

media, cartridges, flash systems for laboratory and Process

Analytical Technology (PAT)

⦁ Size-Exclusion Chromatography (SEC): media and columns

for process purification of proteins and nucleic acids

⦁ Preparative Chromatography (FPLC): columns and media

for laboratory, pilot, and process

⦁ Thin-Layer Chromatography (TLC): plates, adsorbents, TLC

plate cutter, accessories

⦁ High-Pressure Liquid Chromatography (HPLC/UPLC): col-

umns and adsorbents for LC–MS, analytical, semi-prep, prep

⦁ Solid-Phase Extraction (SPE): cartridges and 96 well plates

⦁ Gas Chromatography: capillary columns with low bleeding

and ultra-low bleeding

⦁ Sample Preparation: SPE cartridges and syringe filters


Major Products/Services Tosoh Bioscience offers a comprehensive

line of TSKgel prepacked columns for each of

the major chromatography modes, process

development tools, as well as TOYOPEARL,

TSKgel, and Ca++Pure-HA bulk resins for

laboratory scale purifications and process

chromatography.

Prepacked columns containing TSKgel

media are designed for the analysis of

proteins, peptides, other biopolymers, low

molecular weight compounds, and organic

soluble polymers. The packings in the

columns are either silica-based or polymer-

based material, in particle sizes ranging from

2 μm to 30 μm. Columns are available in

analytical through semi-preparative sizes, in

stainless steel, PEEK®, or glass.

A variety of screening tools composed of

TOYOPEARL and TSKgel process media are

available as an additional tool for convenient

scale-up. In addition, bulk media volumes of

<1 L are offered for process development.

The EcoSEC GPC System and the

EcoSEC High Temperature GPC System are

dedicated GPC instruments developed spe-

cifically for polymer analysis. These all-in-one

systems deliver top performance, reliability,

and superior results.

Facilities Tosoh Bioscience LLC is located in King of

Prussia, Pennsylvania. Supply chain offices

are in Grove City, Ohio. European operations

are in Griesheim, Germany. Asia is served by

Tosoh Corporation in Tokyo, Japan; Shang-

hai, China; and Singapore. Products are man-

ufactured by Tosoh Corporation in Japan.

Tosoh Bioscience LLC

Company Description Tosoh Bioscience LLC is a major supplier of chromatography

products worldwide, particularly to the pharmaceutical, bio-

technology, and polymer industries. The company is a division

of Tosoh Corporation, a global chemical company with head-

quarters and manufacturing facilities in Japan. Tosoh’s portfolio

of over 500 TSKgel® and TOYOPEARL® products encompasses

all common modes of liquid chromatography. We manufacture

analytical HPLC columns and provide bulk chromatographic res-

ins for the purification of biopharmaceutical drugs in commercial

manufacturing processes. Ca++Pure-HA™, a hydroxyapatite resin

for biomolecule purification, is a unique resin from Tosoh in that

it is both the ligand and base bead. Tosoh Bioscience LLC offers

dedicated systems for polymer characterization, the EcoSEC GPC

System and the EcoSEC High Temperature GPC System.

Chief Chromatographic Techniques Supported ⦁ Size exclusion

⦁ Reversed phase, normal phase (hydrophilic interaction)

⦁ Ion exchange

⦁ Hydrophobic interaction

⦁ Affinity

⦁ Mixed-mode

Markets Served Located in King of Prussia, Pennsylvania, Tosoh Bioscience

LLC provides sales and service to pharmaceutical and

biotechnology customers in North and South America.

Tosoh Bioscience, LLC

3604 Horizon Drive

Suite 100

King of Prussia, PA 19406

T E L E P H O N E

(484) 805-1219

(800) 366-4875

F A X

(610) 272-3028

E - M A I L

[email protected]

W E B S I T E

www.tosohbioscience.com


35


1987


Markets Served ⦁ Clinical

⦁ Environmental

⦁ Food safety

⦁ Forensic

⦁ Pharmaceutical

⦁ Veterinary

Major Products/Services ⦁ CLEAN SCREEN® SPE columns

⦁ CLEAN SCREEN FASt® columns

⦁ CLEAN SCREEN XCEL® SPE columns

⦁ CLEAN UP® SPE columns

⦁ STYRE SCREEN® SPE columns

⦁ XtrackT® SPE columns

⦁ ENVIRO-CLEAN® SPE cartridges

⦁ ENVIRO-CLEAN® universal cartridges

⦁ Chlorofiltr® Sorbents

⦁ QuEChERS

⦁ Quick QuEChERS

⦁ SpinFiltr™

⦁ Ultra Flash®

⦁ SELECTRA® HPLC columns

⦁ SELECTRASORB bulk sorbents

⦁ SELECTRA-SIL® derivatizing reagents

⦁ SELECTRAZYME® β-glucuronidase

⦁ ABALONASE™ β-glucuronidase

⦁ SELECT pH buffer pouches

⦁ GC liners

⦁ Positive pressure manifolds

⦁ Vacuum manifolds

Facility UCT is headquartered in Bristol,

Pennsylvania, with a research facility in

Scranton, Pennsylvania. Manufacturing

and distribution sites are in Lewistown,

Pennsylvania and Wexford, Ireland. UCT

is represented worldwide by over 47

partners and distributors.

UCT, Inc.

Company Description UCT is always at the forefront of sample preparation technology

and a leader in chromatography consumables. The company’s

wide range of highly reproducible silica and polymeric SPE

sorbents provide scientists a consistent extraction technique.

UCT is in a unique position within the sample prep and

chromatographic sorbent industry; we are one of the few

manufacturers of chromatographic silane materials. This expertise

provides us with greater control and extensive knowledge of the

chemical processes involved in producing high quality bonded

phases. UCT’s commitment to ensuring customer satisfaction

is accomplished by delivering on our promises: top quality,

reproducible chromatographic and sample prep products, and

unmatched technical support. The company’s sample prep

product lines include SPE cartridges and well plates, flash

chromatography columns, bulk sorbents, QuEChERS tubes,

derivatizing reagents, hydrolyzing enzymes, premeasured buffer

salts, and extraction manifolds; chromatography products include

HPLC columns and GC liners. All products are designed to meet

laboratory’s sample prep and chromatography needs.

Chief Chromatographic Techniques Supported ⦁ Solid-phase extraction

⦁ QuEChERS

⦁ Flash Chromatography

⦁ HPLC

UCT, Inc.

2731 Bartram Road

Bristol, PA 19007

T E L E P H O N E

(215) 781-9255

F A X

(215) 785-1226

E - M A I L

[email protected]

W E B S I T E

www.unitedchem.com


>100


1986


Valco Instruments Co., Inc.

(VICI)

P.O. Box 55603

Houston, TX 77255

T E L E P H O N E

(800) 367-8424

F A X

(713) 688-8106

E - M A I L

[email protected]

W E B S I T E

www.vici.com


US: 365

Europe: 135


1968

Valco Instruments Co., Inc. (VICI)

GC capillary columns, and Dynacal gas

calibration standards. VICI Precision

Sampling manufactures analytical quality

syringes, sampling probes, as well as

standard and custom formed stainless and

special alloy tubing. VICI AG International

produces some Valco products as well as

the Jour line of HPLC fittings and lab safety

products. VICI DBS builds our new line of

laboratory hydrogen, nitrogen and zero air

gas generators.

Our newest products include the C82 line

of UHPLC valves, fittings that permit direct

connection of 360 μm tubing, the Universal

valve actuator, and the C52 platform

integrated HPLC injectors and selectors.

Facility With over 150 state-of-the-art CNC

machines, our instruments and

components are manufactured to exacting

standards at our facilities in Houston,

USA and Schenkon, Switzerland. Our

other manufacturing facilities include VICI

Metronics in Washington, VICI Precision

Sampling in Louisiana and VICI DBS

located in Italy. VICI Canada serves our

neighbor to the north from its offices in

Brockville, Ontario.

Company Description Founded in 1968, VICI is an international group of

companies. Our products can be found in nearly every

national lab, military facility, and university research

department around the globe. For 50 years VICI has driven

the field of chromatography with valves, fittings, detectors

and instruments for precision analytical, biomedical, and

biocompatible applications. We also customize components

and complete systems to improve chromatographic analyses.

Chief Chromatographic Techniques Supported ⦁ High Pressure Liquid Chromatography

⦁ Liquid Chromatography

⦁ Gas Chromatography

⦁ Flow Injection Analysis

⦁ Supercritical Fluid Chromatography

⦁ Gas Permeation Chromatography

⦁ Process Gas Chromatography

Major Products/Services VICI produces a complete line of valves for analytical chemistry.

We specialize in zero dead volume fittings and filters for the

separation sciences and are the original creators of the pulse

discharge dectector (PDD). Our miniPDD consumes less than

one-fifth the amount of helium required by the PDDs used on

complete GC systems. It has similar sensitivity to the original

PDD with a slightly lower dynamic range.

Our VICI Metronics division produces PEEK, FEP, PFA, and

ETFE tubing, ambient temperature gas purifiers, ValcoBond

Everything you need for

Fast GC

VICI Fast Temperature Programmer• Four independently rampable temp zones up to 2500°C/min

VICI Heater Transfer Lines• Multiple size and shape options• Temperature sensor

VICI Columns• Available in any phase either as a fused silica, metal or packed column

VICI Fan Modules• High flow fan size options and voltage options• Multiple mounting options

No more cold or hot spots. Program all the elements simultaneously.

Visit www.vici.com/fast-gc for more information.

Valco Instruments Co. Inc.

Fast GC Product Options1. Individual components open stock

2. Kit for updating or building GC

3. Modular form configuration

4. Custom applied instruments

Fast GC Components

SimDis Analysis


Major Products/Services WTC’s family of instruments includes:

⦁ DAWN HELEOS: An 18-angle SEC–

MALS detector that can be used at

ambient, elevated, and below ambient

temperatures for polymers, particles, and

biopolymers.

⦁ miniDAWN: A SEC–MALS detector ideal

for proteins and peptides and molar

mass materials below 1 million Da.

⦁ μDAWN: The world’s first MALS detector

for UHPLC. Compatible with all UHPLCs

for molar mass and size determinations.

⦁ UT-rEX: The world’s first refractive index

(RI) detector for UHPLC. Operates at the

same wavelength of light as the μDAWN.

⦁ Eclipse system: For separation of

macromolecules and nanoparticles in

solution.

⦁ Optilab TrEX: The most advanced RI

detector in the world with 256 times

the detection power and 50 times

the dynamic range of any other RI

detector. The TrEX can be operated

below or above ambient temperature

and determine dn/dc at the same

wavelength of light as the light-

scattering instrument.

⦁ WyattQELS: A quasi-elastic (dynamic)

light-scattering instrument that can be

interfaced to the DAWN HELEOS or the

miniDAWN TREOS to determine particle

sizes as small as 1 nm.

⦁ ViscoStar viscometer: A state-of-the-

art intrinsic viscosity detector that can

be operated above or below ambient

temperature with its precise Peltier

thermostatic controls.

⦁ WTC Protein Columns: A family of new

silica based columns specifically designed

for SEC–MALS protein applications. These

columns retain all the important features

of a first-class SEC column for protein

separations.

Facility WTC maintains more than 45,000 square

feet of modern electronics, optical

prototype, machine shop, and laboratory

facilities in Santa Barbara, California.

Wyatt Technology Corp.

Company Description Wyatt Technology, a 35-year old family-owned and operated

enterprise, is the world’s largest company dedicated to SEC-

MALS detectors for absolute macromolecular characterization.

Our instrumentation provides the essential techniques for

characterizing macromolecules and nanoparticles in solution, in

order to determine molar mass, size, charge, and interactions.

Wyatt’s extensive applications laboratories and its full-time staff

(including more than 18 PhD scientists) means our customers

receive deep technical expertise and support. To ensure the

personal touch, “Light Scattering University” (LSU) is included

with most of our instruments. We bring you to Santa Barbara

to de-mystify light scattering, work you hard but feed you well,

and, of course, explain how to get the most from your Wyatt

Technology equipment.

Chief Chromatographic Techniques Supported ⦁ UHPLC

⦁ GPC/SEC/GFC

⦁ RPC

⦁ Field-flow fractionation

Markets Served Wyatt’s products are the most widely used multi-angle light

scattering (MALS) detectors in the world. They are employed

in thousands of laboratories including, but not limited to,

companies involved in biotechnology, pharmaceutical

development, and chemical and petrochemical research, in

addition to government national labs and academic facilities.

Wyatt Technology Corp.

6330 Hollister Ave.,

Santa Barbara, CA 93117

T E L E P H O N E

(805) 681-9009

F A X

(805) 681-0123

E - M A I L

[email protected]

W E B S I T E

www.wyatt.com


120


1982

wyatt.com

© 2017 Wyatt Technology and Leo Cullum from cartoonbank.com. All rights reserved. All trademarks and registered trademarks are properties of their respective holders.

That’s the problem with relying on the assumptions of column calibration to characterize macromolecules.

You don’t really know if your standards and your samples are the same! Perhaps that’s why every major

pharmaceutical and biotechnology company, as well as most federal regulatory agencies are switching from

relative methods to Wyatt Technology’s absolute measurements. We’ll show you how to end your dependence

on reference standards, in order to make you and

your products a whole lot more convincing. See our

webinar on Absolute Biophysical Characterization

at wyatt.com/Absolute.


Markets Served Located in Allentown, Pennsylvania, YMC

America, Inc., provides sales and service to

pharmaceutical, biotechnology, biopharma-

ceutical, government, and environmental

customers in North and South America.

Major Products/Services ⦁ YMC-Triart

⦁ YMC-BioPro

⦁ Meteoric Core (Core-shell)

⦁ Chiral, CHIRAL ART: Amylose-C, Cellu-

lose-C, Amylose-SA, Cellulose-SB, Cellu-

lose-SC; Cellulose-SJ; YMC Chiral Series:

NEA (R), (S), α-CD BR, β-CD BR, γ-CD BR

⦁ Reversed-phase, ODS: YMC-Triart C18

and C18 ExRS, Meteoric Core C18 and

C18 BIO; Pro Series: Pro C18, Hydro-

sphere C18, Pro C18 RS; YMC-Pack

ODS Series: ODS-A, ODS-AM, ODS-AQ,

ODS-AL; J’sphere ODS: ODS-H80, ODS-

M80, ODS-L80; YMC-Pack PolymerC18

⦁ Other than ODS: YMC-Triart C8, Phenyl,

and PFP; Meteoric Core C8; Pro C8; Pro

C4; YMCbasic, YMC Carotenoid; YMC-

Pack Series: C8, C4, TMS, Ph (Phenyl),

CN (Cyano), Protein-RP, YMCbasic, YMC

Carotenoid

⦁ Normal phase: YMC-Triart Diol-HILIC;

YMC-Pack Series: SIL, SIL-06, Diol-NP,

CN, PVA-Sil, Polyamine II, NH2, PA-G

⦁ Ion-Exchange, YMC-BioPro Series:

QA (porous quaternary amine anion

exchange), SF (porous sulfopropyl cation

exchange), QA-F (non-porous QA), SP-F

(non-porous SF)

⦁ Size-Exclusion, YMC-Pack Series:

Diol-60, Diol-120, Diol-200, Diol-300;

YMC-GPC

In addition to all of YMC’s analytical chroma-

tography offerings, most chromatography

chemistries are scalable to bulk packing ma-

terials for lab-scale and production-scale puri-

fications. YMC also offers preparative systems

for discovery, pilot-scale, and production.

Facilities YMC America, Inc. – Allentown, Pennsylvania

YMC Co., Ltd. – Kyoto, Tokyo, Japan

YMC Europe GmbH – Dinslaken, Germany

YMC India Pvt. Ltd. – New Delhi, India

YMC China – Shanghai, China

YMC Korea Co., Ltd. – Gyeonggi-do, Korea

YMC Taiwan Co., Ltd. – Taipei City, Taiwan

YMC Singapore Tradelinks Pte, Ltd. –

Singapore

YMC Schweiz GmbH – Basel, Switzerland

YMC America, Inc.

Company Description YMC America, Inc., is the exclusive sales and support channel for

separations leader YMC Co., Ltd., whose products include station-

ary phase chemistries primarily used for high and low pressure

separation science. Stationary phases designed for use with

high performance liquid and supercritical fluid chromatography

techniques are available as stocked standard items and custom

products are available for specialized application needs. Products

bonded to spherical silica, hybrid silica, and polymeric-based par-

ticles ranging in size from 1.9 to 150 μm are available in packed

columns or in bulk chemical packaging formats. YMC America

maintains offices, application support laboratories, and a ware-

house in Allentown, Pennsylvania and manages the applications

support and sub-distribution of YMC products throughout the

Western hemisphere, either through direct contact with its cus-

tomers or via a network of distributors throughout the Americas.

YMC products are available on a world-wide basis.


⦁ UHPLC

⦁ Supercritical fluid chromatography (SFC)

⦁ Chiral chromatography

⦁ Ion-exchange (biomolecules) chromatography

⦁ Size-exclusion chromatography (SEC)

⦁ Preparative chromatography

⦁ HILIC chromatography

⦁ Superficially porous particle (core-shell) chromatography

⦁ Nano–capillary chromatography

⦁ Preparative chromatography systems

⦁ Flow reactor chemistry

YMC America, Inc.

941 Marcon Blvd.

Suite 201

Allentown, PA 18109

T E L E P H O N E

(610) 266-8650

F A X

(610) 266-8652

E - M A I L

[email protected]

W E B S I T E

www.ymcamerica.com


Worldwide: 350


1980

YMC delivers reproducibility in over 30

distinctly different phase chemistries.

Beyond reproducibility, YMC delivers innovation … in hybrid phases,

core-shell technology, chiral separations, ion exchange, and more.

Scalable from UHPLC to the production floor.

Bring your separation challenges to YMC.

www.ymcamerica.com

[email protected]

+1-610-266-8650


ZirChrom

Separations, Inc.

617 Pierce Street

Anoka, MN 55303

T E L E P H O N E

(763) 421-5264

F A X

(763) 421-2319

E - M A I L

[email protected]

W E B S I T E

www.zirchrom.com


1995

ZirChrom Separations, Inc.

Markets Served Pharmaceutical, nutraceutical, basic sci-

ence research, antibody purification, food

and cosmetics, chemical manufacturing

(pesticides, polymers), biological research

(phosphopeptide enrichment), and chiral

separation.

Major Products/Services ZirChrom offers a full line of zirconia and

titania-based HPLC products for chromato-

graphic method development. ZirChrom

HPLC products offer unique selectivity, ex-

acting reproducibility, high efficiency, and

ultra-stability. All phases are available in

packed, prep, semi-prep, bulk, microbore,

and SPE formats:

Reversed Phase (Diamondbond®-C18,

ZirChrom®-CARB, ZirChrom®-EZ,

ZirChrom®-MS, ZirChrom®-PBD,

ZirChrom®-PS, Sachtopore®-RP);

Ion-Exchange (ZirChrom®-PEZ,

ZirChrom®-SAX, ZirChrom®-SHAX,

ZirChrom®-WAX, ZirChrom®-WCX);

Other/Specialty (ZirChrom®-PHASE,

Rhinophase®-AB, Sachtopore®-NP);

Chiral (ZirChrom®-Chiral(S)LEU,

ZirChrom®-Chiral(R)NESA, ZirChrom®

-Chiral(S)NESA, ZirChrom®-Chiral(S)PG,

ZirChrom®-Chiral(R)PG, ZirChrom®

-CelluloZe).

Facilities ZirChrom is headquartered in Anoka,

Minnesota, and has distributors

worldwide.

Company Description ZirChrom Separations, Inc. is a company that was formed

in 1995 and is located in Anoka, Minnesota. ZirChrom

manufactures a full line of zirconia and titania based high

performance chromatographic materials for the analytical

analysis of compounds primarily by high performance liquid

chromatography (HPLC).


⦁ Semi-prep chromatography

⦁ Prep chromatography

⦁ Ion exchange chromatography

⦁ Reversed phase chromatography

⦁ HILIC

⦁ Sample prep

⦁ Multi-modal chromatography

⦁ Pseudo-affinity chromatography

⦁ Chiral chromatography

⦁ Bulk materials

⦁ Microbore-HPLC

⦁ UHPLC/Sub-2 micron

APPLICATION NOTES – DECEMBER 2017 55

MEDICAL/BIOLOGICAL

Presented is a rapid, three step SPE procedure, that includes a

concentration step, for the identifi cation and quantifi cation of fentanyl

and its major urinary metabolite norfentanyl, along with 11 additional

synthetic opioid compounds: acryl fentanyl, desmethyl U-47700,

furanyl fentanyl, methoxyacetyl fentanyl, remifentanil, tetrahydrofuran

fentanyl, U-47700, U-50488, U-51754, W-15, and W-18.

Procedure

1. Sample Pretreatment – Hydrolysis

a) To 1 mL of urine sample add 1 mL of Abalonase™+ working

enzyme stock solution (10,000 Fishman units/mL).

b) Add internal standard(s).

c) Gently mix by inversion or vortex 10 to 15 s prior to use.

d) Hydrolyze for 15 min to one h from room temperature to 70 °C.

2. SPE Procedure

a) Apply the sample to the SPE cartridge (if required, use a low

vacuum to draw the sample through at ≤3 mL/min).

b) Wash Cartridge:

1. 1 × 2 mL D.I. H2O.

2. 1 × 2 mL 75:25 D.I. H2O: MeOH.

3. Dry column under full vacuum or pressure for 10 min.

c) Elution:

1. Elute with 1 × 2 mL CH2Cl

2/IPA/NH

4OH (75:20:5).

2. Evaporate the sample to dryness under a gentle stream

of nitrogen.

3. Reconstitute in 100 μL 95:5 D.I. H2O: MeOH and vortex

for 1 min.

4. Transfer sample to autosampler vial containing a low

volume insert.

Instrumental

LC–MS/MS: Shimadzu Nexera X2 LC-30AD coupled to a

Shimadzu LCMS-8050

Column: UCT Selectra® PFPP HPLC column

Analysis of Fentanyl

and Designer Fentanyl

Derivatives in Urine Using

SPE and HPLC–MS/MSDanielle Mackowsky, UCT, LLC

UCT, LLC2731 Bartram Rd, Bristol, PA 19007

Tel: (800) 385-3153

Website: www.unitedchem.com

Extraction/Analytical Materials

CSXCE106Clean Screen® XCEL I

130 mg / 6 mL SPE Cartridge

ASFBETA-GLUC

AbalonaseTM +

Purifi ed Beta-glucuronidase with enhanced

sulfatase activity

SLPFPP100ID21-3UMSelectra® PFPP HPLC column

100 × 2.1 mm, 3 μm

SLPFPPGDC20-3UMSelectra® C18 guard column

10 × 2.1 mm, 3 μm

100 × 2.1 mm, 3 μm

Guard column: UCT Selectra® PFPP guard

Column - 10 × 2.1 mm, 3 μm

Injection volume: 5 μL

Mobile phase A: D.I. H2O + 0.1% formic acid

Mobile phase B: MEOH + 0.1% formic acid

Column fl ow rate: 0.30 mL/min

Results

Recovery and RSD% from spiked coffee samples

Compound 1 ng/mL 5 ng/mL 15 ng/mL

Acryl Fentanyl 92 82 94

Desmethyl U-47700 95 89 82

Fentanyl 99 100 83

Furanyl Fentanyl 97 80 91

Methoxyacetyl Fentanyl 94 91 97

Norfentanyl 93 88 88

Remifentanil 90 92 91

Tetrahydrofuran Fentanyl 100 84 85

U-47700 94 85 94

U-50488 99 96 75

U-51754 96 93 83

W-15 82 98 95

W-18 82 98 90

Conclusion

A fast and effective method was developed for the determination of

fentanyl and other synthethic opiate compounds in urine samples. All

analytes of interest were extracted using a Clean Screen XCEL® I col-

umn. Analysis of the samples was performed by LC–MS/MS utilizing a

Selectra® PFPP HPLC column which allowed for improved separation

of furanyl fentanyl and fentanyl, when compared to other column phas-

es. Absolute recoveries ranged from 75–100% for all three control levels

tested. With the unfortunate (and often unaware) abuse of synthetic opi-

ates throughout the United States, it is critical that forensic laboratories

have accurate and rapid SPE methods for the identifi cation of this class

of compounds. The proceeding method will be of great use as drugs

with similar structures start to be found in future casework.

56 APPLICATION NOTES – DECEMBER 2017

MEDICAL/BIOLOGICAL

Methods for monitoring alcohol consumption biomarkers EtG

and EtS are generally limited by poor retention and coelution

with matrix interferences, as well as by long analysis times and

short column lifetimes. The dilute-and-shoot EtG/EtS LC–MS/MS

analysis developed here using the novel Raptor EtG/EtS column

easily resolves EtG and EtS from matrix interferences, provid-

ing consistent, accurate results for high-throughput labs testing

human urine samples for alcohol consumption.

Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are important bio-

markers of alcohol use. The detection of these metabolites offers

many advantages for abstinence monitoring, including a three-day

detection window, good stability in properly stored specimens, and

analytical specifi city. However, typical methods for EtG/EtS analysis

have several shortfalls: poor resolution of EtG and EtS from matrix

components, long run times that limit sample throughput, and short

column lifetimes.

In this study, a simple dilute-and-shoot method was developed,

validated, and applied to patient samples for EtG/EtS LC–MS/MS

analysis in human urine. The method was developed on a Raptor

EtG/EtS column because it provides the retention characteristics that

are needed to consistently elute the target analytes away from matrix

interferences. In addition, it is rugged and long-lasting, which is

benefi cial to high-throughput laboratories.

Experimental Conditions

Standard and Sample Preparation

Human urine (alcohol free) was fortifi ed with EtG and EtS in order to

prepare calibration standards and QC samples. The concentration of

calibration standards ranged from 50–5000 ng/mL for both analytes.

Four QC levels were prepared at 50, 150, 750, and 4000 ng/mL.

Aliquots (50 μL) of urine were diluted with 950 μL of the work-

ing internal standard (100 ng/mL EtG-d5 and 25 ng/mL EtS-d5 in

0.1% formic acid in water). Samples were vortexed at 3500 rpm for

10 s to mix followed by centrifugation at 3000 rpm for 5 min at 10 °C.

Additional double blanks were extracted for column equilibration.

Defi nitive EtG/EtS

LC–MS/MS Analysis: A Rugged

4-Min Method for High-

Throughput LaboratoriesJustin Steimling and Frances Carroll, Restek Corporation

Time (min)

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0

MatrixInterference

MatrixInterference

MatrixInterference

MatrixInterference

EtG

EtG

EtS

Injection 1

Injection 1,000

EtS

Figure 1: Rugged Raptor EtG/EtS columns provide reliable results for EtG/EtS LC–MS/MS analysis allowing more samples to be analyzed be-tween column changes.

Table I: Analyte transitions

Analyte Precursor Ion Product Ion Product Ion

EtG-d5 225.9 84.9 –

EtG 220.8 84.9 74.8

EtS-d5 129.7 97.7 –

EtS 124.7 96.8 79.7

LC–MS/MS Analysis

In order to ensure good response, peak shape, and retention time

consistency, the analytical column was conditioned prior to use with

30 matrix injections that were run through the full gradient program.

Instrument parameters for EtG/EtS LC–MS/MS analysis are shown

below and the analyte transitions are given in Table I.

Analytical column: Raptor EtG/EtS, 2.7 μm, 100 mm × 2.1 mm

(cat.# 9325A12)

Guard column: UltraShield UHPLC precolumn fi lter, 0.2 μm frit

(cat.# 25809)

Mobile phase A: 0.1% Formic acid in water

Mobile phase B: 0.1% Formic acid in acetonitrile

Gradient Time (min) %B

0.00 5

2.50 35

2.51 5

4.00 5

Flow rate: 0.5 mL/min


Column temp.: 35 °C

Ion mode: Negative ESI

Results

Chromatographic Performance

As shown in Figure 1, a fast, 4-min EtG/EtS LC–MS/MS analysis was

obtained from the direct (dilute-and-shoot) injection of supernatant.

Both EtG and EtS are clearly resolved from matrix interferences, mak-

ing accurate peak identifi cation an easy task. Even after 1000 sample

injections on a Raptor EtG/EtS column, all chromatographic peaks


MEDICAL/BIOLOGICAL

Restek Corporation110 Benner Circle, Bellefonte, PA 16823

tel. (800) 356-1688, fax (814) 353-1309

Website: www.restek.com

Time (min)

0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0

EtG

(overlay of 5,000 ng/mL standard)

Matrix double

blank injection with

post-column EtG

infusion.

Figure 2: EtG elutes outside the matrix suppression zones (double blank injection with post-column EtG infusion).

maintained the initial peak shape, retention time, and response.

Linearity

Using linear 1/x weighted regression for EtG and EtS, both compounds

showed good linearity with r2 values of 0.999 or greater over a calibra-

tion range of 50–5000 ng/mL. Accurate calibration down to 50 ng/mL

allows low-level positive results to be reported with confi dence.

Accuracy & Precision

Precision and accuracy analyses were performed on three different

days. The method accuracy was demonstrated to be within 6.3%

of the nominal concentration for all QC levels for both EtG and EtS.

The %RSD was 1.28–9.19% and 4.01–6.82% for intra- and inter-

run, respectively, at the QC LLOQ. The %RSD was 0.675–7.78%

and 1.00–4.99% for intra- and inter-run, respectively, at the QC low,

mid, and high levels, indicating good method precision for EtG/EtS

LC–MS/MS analysis (Table II).

Matrix Effect

Samples prepared in matrix show approximately 80% (EtG) and

100% (EtS) of the signal obtained when samples are prepared in sol-

vent across all QC levels, demonstrating that matrix effect is minimal.

The sensitivity and consistency of the EtG response at the LLOQ is

only possible because retention on the Raptor EtG/EtS column was

optimized so that EtG elutes outside the zones of matrix suppression

(Figure 2). Because the optimal EtG elution time was established here

during method development, clinical labs adopting the method can

be confi dent that matrix suppression has already been minimized.

This, in combination with the established calibration range and LLOQ,

demonstrates that the method provides excellent sensitivity for EtG,

which is generally harder to detect at low levels than EtS.

Sample Analysis

The robustness of the method was further tested by monitoring fi ve

patient samples with positive results for both EtG and EtS in the lower

end of the linear range across multiple instrument platforms and nine

Table II: Accuracy and precision of QC samples

QC LLOQ (50 ng/mL) QC Low (150 ng/mL)QC Mid

(750 ng/mL)

QC High

(4000 ng/mL)

Analyte

Average

Conc.

(ng/mL)

Average

Accuracy

(%)

%RSD

Average

Conc.

(ng/mL)

Average

Accuracy (%)%RSD

Average

Conc.

(ng/mL)

Average

Accuracy

(%)

%RSD

Average

Conc.

(ng/mL)

Average

Accuracy (%)%RSD

EtG 51.2 102 6.82 143 95.2 4.99 749 99.8 3.68 3949 98.7 2.04

EtS 46.9 93.7 4.01 143 95.6 1.42 762 102 1.00 3958 98.9 1.59

Table III: Inter-run precision of patient samples across

multiple instrument platforms and nine column lots

Patient

Sample

Average EtG

Concentration

(ng/mL)

%RSD

Average EtS

Concentration

(ng/mL)

%RSD

A 216 6.13 78.0 3.56

B 1167 4.81 300 3.24

C 98.2 9.76 82.4 4.01

D 319 8.33 233 3.63

E 247 11.2 163 8.70

lots of Raptor EtG/EtS columns. The precision of the results (n=9) was

found to range from 3.24–11.2% for both analytes over multiple days

and sample preparations, indicating excellent robustness and ease of

method transfer (Table III). High-throughput labs in particular will benefi t

from implementing this rugged EtG/EtS LC–MS/MS analysis method as

highly consistent performance is seen across a wide range of scenarios.

Conclusions

It was demonstrated that the Raptor EtG/EtS column is excellent for

the rapid and accurate analysis of EtG and EtS in human urine. Iso-

baric matrix interferences are easily resolved, preventing issues with

peak identifi cation and quantitation. In addition, superior sensitiv-

ity for EtG is achieved because it elutes outside the zones of matrix

suppression that are typically observed with dilute-and-shoot assays.

With a fast and simple sample preparation procedure and only 4 min

of chromatographic analysis time, the EtG/EtS LC–MS/MS method

established here provides accurate, high-throughput monitoring of

alcohol consumption. For the full article, visit www.restek.com and

enter “CFAN2736” in

the search box.

FOOD & BEVERAGE


A sensitive determination method with automated solid-

phase extraction is presented and the recovery rates are

compared with manual extraction. The automated extraction

was developed with the SPE robot FREESTYLETM SPE module

(LCTech GmbH). According to the European Regulation (EC)

No 470/2009 CHROMABOND® HLB was applied for solid-phase

extraction (1). An HPLC–MS/MS method with the biphenyl

phase NUCLEODUR® π2 was used for the subsequent analysis.

Chloramphenicol is a broad-spectrum antibiotic against Gram-pos-

itive and Gram-negative bacteria. It has many side effects. Thus, it

is only used nowadays as reserve antibiotic (2). For warranty of the

food safety, many countries declare limits for pharmaceuticals in

animal products. The necessary determination methods demand

a high-performance solid-phase extraction to reliably detect and

quantify residues in trace level with the subsequent analysis.

Automated Solid-Phase Extraction (3)

Sample pretreatment: Weigh out 5 g of honey. Add 4 mL water and shake

rigorously for 30 s. Spike with 1 mL standard solution (chloramphenicol,

chloramphenicol-d5 , c = 5 ng/mL in methanol) and shake rigorously for

30 s. Add 15 mL ethyl acetate and shake rigorously for 30 s. Centrifuge

at 3000 rpm for 10 min. Take 12 mL of supernantant and evaporate

extracts to dryness at 40 °C under a stream of nitrogen. Redissolve

residue in 10 mL water.

Extraction:

SPE robot: FREESTYLETM SPE module, LCTech

SPE column: CHROMABOND® HLB, 3 mL, 200 mg, MACHEREY-

NAGEL REF 730924

Column conditioning: 3 mL methanol (dispensing speed 1 mL/min),

then 5 mL dest. water (dispensing speed 1 mL/min)

Sample application: 9 mL pretreated sample (dispensing speed

3 mL/min over sample loop)

Washing: 10 mL dest. water (dispensing speed 3 mL/min)

Drying: 100 mL air (dispensing speed 100 mL/min)

Elution: 5 mL ethyl acetate – methanol (80:20, v/v)

Drying: 100 mL air (dispensing speed 100 mL/min)

Evaporation: under nitrogen, 40 °C

Reconstitution: in 1 mL dest. water – acetonitrile (95:5, v/v)

Determination of Chloramphenicol in Honey with Automated

Solid-Phase Extraction and HPLC–MS/MS DetectionHans Rainer Wollseifen, Johannes Brand, and Detlef Lambrecht, MACHEREY-NAGEL GMBH & CO. KG

Figure 1: Chromatogram of spiked honey sample (1 μg/kg).

FOOD & BEVERAGE


Manual Solid-Phase Extraction

After identical sample pretreatment as for automated solid-phase

extraction the sample was manually extracted by a vacuum manifold

under comparable SPE conditions.

Subsequent Analysis: HPLC–MS/MS (4)

HPLC column: EC 150/2 NUCLEODUR® π2, 5 μm, MACHEREY-

NAGEL REF 760624.20

Eluent A: dest. water

Eluent B: acetonitrile

Gradient: 5–95% B in 7.5 min, 95% B for 1 min, 95–5% B in

1 min, 5% B for 5 min


Temperature: 35 °C


MS/MS detection: API 5500 (AB Sciex), ion source ESI, negative ion-

ization mode, scan type MRM (chloramphenicol: [M-H]- 320.9, 152.0

(Q1), 256.0 (Q2); chloramphenicol-d5: [M-H]- 325.9, 156.6 (Q1),

261.8 (Q2)), curtain gas 35 psig, ion spray voltage -4500 V, temperature

450 °C, nebulizer gas 45 psig, turbo gas 45 psig, CAD medium

Results

Solid-phase extraction of chloramphenicol from the matrix honey

with CHROMABOND® HLB results in an outstanding recovery. The

recovery rates presented in Table I show that higher recovery rates

up to 90% with very good reproducibility (< 5%) are received by

automation with the SPE robot FREESTYLETM SPE module. A cor-

rection with an internal standard is especially advantageous for the

manual sample preparation. Figure 1 shows the chromatogram of

a sample extract from spiked honey. A defi nite identifi cation and

quantifi cation is possible due to a clear separation of matrix interfer-

ences from the analyte peak.

The liquid chromatographic method could be accelerated by us-

ing a shorter HPLC column due to good retention of chlorampheni-

col by the additional π-π-interactions of NUCLEODUR® π2.

Conclusion

A determination method for chloramphenicol in honey has been

developed, which receives very good results—especially by the

automation of solid-phase extraction.

References

(1) Regulation (EC) No 470/2009 of the European Parliament and of the Council.

(2) Side-effects of Chloramphenicol and Aureomycin, Br Med J., 384–2, 388–392

(1951).

(3) Application No. 306350, Macherey-Nagel, available from www.mn-net.

com/apps

(4) Application No. 128140, Macherey-Nagel, available from www.mn-net.

com/apps

MACHEREY-NAGEL GMBH & CO. KGNeumann-Neander-Str. 6–8, 52355 Düren, Germany

Tel. +49-(0)2421-969-0

Website: www.mn-net.com

Table I: Comparison of recovery rates from automated to manual solid-

phase extraction

(%) Recovery

rates

Chloramphenicol Chloramphenicol

(corrected by

int. standard)

Chloramphenicol-d5

Automated SPE 86.8 ± 4.4 (n = 4) 90.8 ± 4.6 (n = 4) 95.6 ± 4.5 (n = 4)

Manual SPE 74.6 ± 2.7 (n = 3) 92.9 ± 3.4 (n = 3) 80.3 ± 4.7 (n = 3)

FOOD & BEVERAGE


Fumonisins are naturally occurring Mycotoxins produced by

Fusarium mold species. Fumonisins contamination happens

worldwide in many agricultural commodities, especially in corn,

and is usually associated with dry and hot weather followed by

periods of high humidity. Out of more than 10 types of Fumoni-

sins, Fumonisin FB1 is the most prevalent and toxic, followed by

Fumonisins FB2 and FB3. Fumonisins are classifi ed as possibly

carcinogenic for humans and also cause health problems in ani-

mals, especially in equids and swine. FDA sets total Fumonisins

limits in human foods between 2–4 μg/g and in animal feed be-

tween 5–100 μg/g.

Since Fumonisins don’t have a chromophore and don’t fluo-

resce, derivatization is needed to achieve the required sensitivi-

ty of detection. We developed a fast and sensitive HPLC method

with post-column derivatization that is capable of analyzing Fu-

monisins in grains and animal feed at levels as low as 0.01 μg/g.

Method

Analytical Conditions

Column: Mycotox reversed-phase C18, 4.6 × 250 mm

(Part No: 1612124)

Temperature: 40 °C


Mobile phase: Eluant A: dilute 1 mL of formic acid to 1 L

with D.I. water

Eluant B: MeOH

Injection volume: 10–50 μL

Analysis of Fumonisins in Grains and FeedsMaria Ofi tserova and Sareeta Nerkar, Pickering Laboratories, Inc.

Figure 1: Chromatogram of corn sample contaminated with 0.17 μg/g of FB1 and 0.03 μg/g of FB2.

Figure 2: Chromatogram of mixed feed sample contaminated with 0.08 μg/g of FB1 and 0.02 μg/g of FB2.

-- ----

--

FB1

FB2

0 2 4 6 8 10 12 14 min

---

-- ----

--

.

FB1

FB2

0 2 4 6 8 10 12 14 min

Post-Column Conditions

Post-column system: Pinnacle PCX

Heated reactor volume: 1.4 mL

Temperature: 65 °C

Reagent: 950 mL GA 104, 300 mg OPA, 2 g

Thiofl uor, 3 mL of 30% Brij 35 solution

Reagent fl ow rate: 0.4 mL/min

Detection: FLD, λEX

=335 nm, λEM

= 440 nm

Table I: HPLC Gradient

Time Eluent A, % Eluent B, %

0 45 55

2 45 55

9 30 70

14 10 90

16 10 90

16.1 45 55

22 45 55

FOOD & BEVERAGE


Pickering Laboratories, Inc.1280 Space Park Way, Mountain View, CA 04043

tel. (800) 654-3330, (650) 694-6700

Website: www.pickeringlabs.com

Figure 3: Chromatogram of barley sample spiked with 0.2 μg/g of FB1 and 0.07 μg/g of FB2.

-- ----

--

FB1

FB2

0 2 4 6 8 10 12 14 min

Figure 4: Chromatogram of saffl ower seeds sample spiked with 0.2 μg/g of FB1 and 0.07 μg/g of FB2.

-- ----

--

FB1

FB2

0 2 4 6 8 10 12 14 min

Table II: Analysis of Fumonisins

SampleFB1 Found in

Sample

FB2 Found in

Sample

FB1 Spike

Concentration

FB2 Spike

ConcentrationFB1 Recoveries FB2 Recoveries

Barley 0 μg/g 0 μg/g 0.2 μg/g 0.07 μg/g 92.3% 87.5%

Milo 0.04 μg/g 0.01 μg/g 0.2 μg/g 0.07 μg/g 85.4% 81.8%

Saffl ower seeds 0 μg/g 0 μg/g 0.2 μg/g 0.07 μg/g 92.0% 80.8%

Corn 0.17 μg/g 0.03 μg/g 0.4 μg/g 0.13 μg/g 91.7% 88.7%

Oats 0 μg/g 0 μg/g 0.2 μg/g 0.07 μg/g 91.5% 83.3%

Mixed feed 0.08 μg/g 0.02 μg/g 0.3 μg/g 0.1 μg/g 88.0% 80.6%

Sample Extraction and Clean-Up

Immunoaffi nity clean-up columns: FumonitestTM (WB (Vicam)

Extraction solution: Water–methanol (20:80)

PBS solution: dilute 100 mL of 10X PBS (Vicam, P/N G1113) to 1 L

with DI water

To 25 g of fi nely ground sample add 2.5 g of NaCl and 50 mL of

extraction solution. Blend at high speed for 5 min, fi lter through

fl uted fi lter. Take 10 mL of extract and add 40 mL of PBS solution,

mix well, fi lter through microfi ber fi lter. Load 10 mL of diluted extract

to Immunoaffi nity column, let the solution pass through at the

fl ow rate about 1–2 drops/s. Wash the column with 10 mL of PBS

solution, elute with 1 mL of methanol followed by 1 mL of DI water.

Evaporate the solution to dryness under the stream of nitrogen,

reconstitute in 1 mL of methanol–water (50:50). Inject 10–50 uL.

ENVIRONMENTAL


The US EPA monitors a variety of chemicals in water that

may cause harm to humans or wildlife to minimize exposure.

Method 625 was developed by the Offi ce of Science and

Technology in the Clean Water program to allow the moni-

toring of a large suite of semivolatile chemicals in waste-

water for compliance with the National Pollution Discharge

Elimination System (NPDES). NPDES is a system of permit-

ting that defi nes the characteristics of water that is released

into a waterway, defi ned by industrial category. The permit-

ting levels are set depending on the waterway’s use. If the

waterway is used for recreation or is an important wildlife

habitat, the limit may be set lower.

The original method was developed in the early 1980s and has been

updated several times since then to allow the use of more mod-

ern technology. The latest update has taken place over the last few

years and was proposed in a Method Update Rule (MUR) in 2015

which was just published in the Federal Register August 28, 2017

and became effective September 27, 2017 (1). The latest version

of the method includes a larger suite of analytes (up to 364) and

an extensive set of labeled surrogates to better monitor the method

performance throughout the sample preparation and analysis step.

This application note will present the data collected as part of

the demonstration of disk solid phase extraction validation for US

EPA method 625.1. Nine different wastewater matrices were evalu-

ated and tested against the criteria listed in Table 6 of the method.

Sample preparation was performed using the Atlantic® One-pass

system, where the water sample is passed through a solid phase

extraction (SPE) disk and carbon cartridge once, rather than twice

with a pH change between loadings. Automation of the process

was achieved using the SPE-DEX® 4790 system (superseded by

SPE-DEX 5000). Table I shows the matrix spike and matrix spike

duplicate results for a centralized waste treatment point source

(437 NPDES category) sample, for selected compounds. The spike

recovery and agreement between the duplicates was within criteria

for most all analytes. Method detection limits, initial demonstration

of compliance, and other wastewater matrices are shown in the full

application note (2).

References

(1) Method 625.1, December 14 revision, can be found in the MUR, Febru-

ary 19, 2015. Or downloaded here: https://nepis.epa.gov/Exe/ZyPDF.cgi/

P100LVHC.PDF?Dockey=P100LVHC.PDF

(2) Application Note 117, “Validation of Horizon Technology Disk Extrac-

tion Technology for US EPA Wastewater Method 625.1,” available from

www.horizontechinc.com.

Validation of Horizon Technology Disk Extraction Technology for US EPA Wastewater Method 625.1Zoe Grosser*, Alicia Cannon*, Michael Ebitson*, Melissa Lever*, Nic Rasnake†, Jessica Bowker†, Allen Fuller†, and Chris Johnson†, *Horizon Technology, and †ESC Lab Sciences

Horizon Technology, Inc.16 Northwestern Drive, Salem, NH 03079

tel. (603) 893-3663

Website: www.horizontechinc.com

Table I: Centralized waste treatment point source (437)

matrix spike and spike duplicate results

CompoundRecovery

(%)

Range

P,Ps(%)

Pass/

Fail

RPD

(%)

RPD (%)

Limit

Pass/

Fail

Fluorene 99.6 59-121 Pass 6.89 38 Pass

Hexachlorobenzene 110 D-152 Pass 9.10 55 Pass

Hexachloro-1,3-butadiene 99.6 24-120 Pass 4.09 62 Pass

Hexachloroethane 61.6 40-120 Pass 16.1 52 Pass

Indeno(1,2,3-cd)pyrene 73.5 D-171 Pass 4.54 99 Pass

Isophorone 100 21-196 Pass 2.71 93 Pass

Naphthalene 96.0 21-133 Pass 2.49 65 Pass

Nitrobenzene 94.2 35-180 Pass 0.79 62 Pass

N-Nitrosodi-n-propylamine 113 D-230 Pass 2.55 87 Pass

Phenanthrene 97.2 54-120 Pass 7.33 128 Pass

Pyrene 90.9 52-120 Pass 8.40 49 Pass

1,2,4-Trichlorobenzene 90.2 44-142 Pass 5.68 50 Pass

PHARMA/DRUG DISCOVERY


A revolutionary new inline static mixer has been developed and

specifi cally tailored to meet the exacting demands of HPLC and

UHPLC systems. Poor mixing of two or more mobile phase sol-

vents results in a high signal to noise ratio and, thus, decreased

sensitivity. The test data presented demonstrates that superior

mixing is achieved while minimizing the internal volume in var-

ious gradient test conditions, such as unparalleled mixing for

trifl uoroacetic acid (TFA) and water and acetonitrile gradients.

Low mixing effi ciency, resulting in poor signal-to-noise ratios, has

plagued the chromatography world when it comes to limits of

detection and sensitivity. If poor mixing is present, baseline noise

will appear as a sine wave (rise and fall) of the detector signal

versus time. Poor mixing will also broaden and create asymmetrical

peaks resulting in reduced column effi ciency and peak resolution.

The ideal static mixer will combine the advantages of high mixing

effi ciency, low dead volume and low pressure drop, while minimizing

the volume and maximizing the throughput of the system.


The following HPLC conditions and test setup were employed to

measure the system baseline noise and to compare the relative

performance of various static mixers. Figure 1 shows a schematic

diagram of a typical HPLC and UHPLC system confi guration. Static

mixer testing was performed by locating the Mott PerfectPeak® static

mixer immediately downstream of the pump and upstream of the

sample injector and HPLC column. For this study, mixing effi ciencies

were performed by bypassing the sample injector and column using

a capillary tube between the static mixer and the UV detector to

create back pressure.

The HPLC system utilized for this testing was an Agilent 1260

Series HPLC with a UV detector controlled using Agilent Chemstation

Software. Table I contains the setup conditions used in this study.

Conclusion

This application note demonstrates that greater mixing effi ciency

is exhibited with every Mott PerfectPeak® static mixer volume

increase with respect to the system with no mixer. Greater than

95% reduction in baseline noise was achieved with the largest size

mixer which will result in improved limits of detection and sensitivity.

Reducing HPLC and UHPLC System Noise and Volume with

High-Performance Static Mixers

James K. Steele, PhD, Kenneth L. Rubow, PhD, and Christopher J. Martino, Mott Corporation

Mott Corporation84 Spring Lane, Farmington, CT 06032

tel. 1 (860) 747-6333; email: [email protected]: www.mottcorp.com

Figure 1: Schematic diagram of the low-pressure gradient experimental test system.

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0 1 2 3 4 5

Ab

sorb

an

ce (m

AU

)

Time (Minutes)

Relative Performance - Mott 25, 50, 100, 150, & 300 !L

Mott 300 �L

Mott 150 �L

Mott 100 �L

Mott 50 �L

Mott 25 �L

No Mixer

Figure 2: Plots showing offset HPLC UV detector signal versus time for this study showing baseline noise with no mixer, and Mott 25 μL, 50 μL, 100 μL, 150 μL, and 300 μL mixers.

Table I: HPLC configuration

Pump speed 0.25 mL/min through 1.0 mL/min

Solvent A 20 mM ammonium acetate in DI water

Solvent B 80% Acetonitrile (ACN) / 20% DI water

Solvent ratio 20% A / 80% B

Detector 220 nm

Flow rate 0.5 mL/min

POLYMER


Phthalates are widely used as plasticizers in different industries to

enhance product durability. It has been determined that many of the

phthalates have a detrimental effect on human health. Six phthalates

(shown in Table I) are now regulated by the EU (Directive 2005/84/EC)

and the US (Consumer Product Safety Improvement Act, Section 108).

All the past methodologies for the determination of these phthalates

are based on solvent extraction, fi ltration, and concentration. These tra-

ditional techniques are cumbersome, time consuming, and suffer from

analyst-to-analyst variability while producing data of limited value.

In response to these analytical challenges and in conjunction with

the ASTM, Frontier Laboratories developed a technique using the Multi-

Shot Pyrolyzer coupled with a GC–MS system for analyzing phthalates

in PVC. This technique provides scientists with a “method map” for

identifying phthalates both qualitatively and quantitatively. First, Evolved

Gas Analysis (EGA)-MS is used to determine the optimal thermal zone

in which the target compounds evolve as the sample is heated. Then,

a simple, one-step thermal desorption (TD)-GC–MS technique is used

for the analysis. This application note details the fi rst step of the “meth-

od map” for identifying the thermal desorption temperature zones of

phthalates in PVC.

A sample consisted of small pieces of a PVC toy was taken from 10

different locations. They were dissolved in 1 mL of THF (50 mg/mL). Six

different phthalates were added to the THF solution. 10 μL of the THF

spiked solution was added to a sample cup; the solvent was evaporated

leaving a thin fi lm of the sample on the surface of the cup (ca. 0.5 mg).

Figure 1 shows the obtained EGA thermogram and extracted ion

chromatograms (EICs) that are used to determine the thermal zone for

each of the phthalates and the hydrogen chloride that is generated as

PVC is heated. Using average mass spectrum, the fi rst peak is identifi ed

as DINCH (1,2 cyclohexane dicarboxylic acid di-isononyl ester). It is

critical to ensure that the ions used to monitor the six phthalates can be

differentiated from ions produced by non-target additives, like DINCH.

The EICs clearly show that the temperature range in which all six phthal-

ates are thermally desorbed is 100 to 350 ºC.

Pyrolyzer: 100–600 ºC (20 ºC/min)

GC oven temp.: 300 ºC,

EGA tube: UADTM-2.5N (L=2.5 m, i.d.=0.15 mm)

Column fl ow rate: 1 mL/min; He, Split ratio: 1/20

Inj. port temp.: 320 ºC

This work clearly shows that the Frontier Multi-Shot Pyrolyzer is a viable

technique in analyzing phthalates in many industries. Today, more

laboratories are integrating the Frontier Multi-Shot Pyrolyzer into their

mainstream analytical protocols due to the advantages and immediate

quality improvements this technique provides. The Frontier Multi-Shot

Pyrolyzer guarantees reproducibility and accuracy while all surfaces

in the sample path are quartz deactivated using a proprietary process.

There is NO transfer line and NO cross contamination. The system also

increases laboratory productivity as sample preparation takes much

less time than traditional techniques that require solvent extraction.

Analysis of Phthalates in Polyvinyl Chloride (PVC) by Thermal

Desorption GC–MS: Determination of Thermal Desorption

Temperature Zone by Evolved Gas Analysis (EGA)Rojin Belganeh and William Pipkin, Frontier Laboratories (Koriyama, Japan)

Quantum AnalyticsFoster City, California, United States

tel. (650) 312-0900

Website: http://go.lqa.com/pyrolyzer-info Figure 1: TIC thermogram and extracted mass chromatograms of PVC, to which 1% of each phthalate was added

Table I: Use restricted phthalates (1% upper limit by

directive 2005/84/EC

Di(2-ethylhexyl) phthalate (DEHP)

Dibutylphthalate (DBP)

Butylbenzylphthalate (BBP)

Diisononylphthalate (DINP)

Diisodecylphthalate (DIDP)

Di(n-octyl)phthalate (DNOP)

GENERAL


The Hamilton PRP-C18 is a column designed to provide high-

effi ciency reversed phase separations over an extended col-

umn life in nearly any mobile phase or pH. The rigid sta-

tionary phase has excellent mechanical and thermal stability

(> 100 °C), does not experience shrinkage or swelling and is

completely inert to most conditions commonly encountered

in reversed phase chromatography. In this study the pH sta-

bility of the PRP-C18 is evaluated. Even after prolonged ex-

posure to concentrated (1 molar) NaOH and H2SO4 there was

no measurable deterioration in performance.

Octadecyl silane (ODS) is the prevailing stationary phase in reversed-

phase HPLC. Despite a well-celebrated, widespread use in chroma-

tography, traditional ODS columns are not without limitations. Acidic

conditions promote hydrolytic stripping of octadecyl functionalization,

while alkaline conditions (pH > 7) attack the silica bed, both of which

are principle sources for anomalous peak shape and shifting retention

times that progressively worsen over the life of the column.

The PRP-C18 reversed phase column has similar mechanical

stability (up to 5000 psi) and separation effi ciency to that of tradi-

tional ODS, but without many of the chemical restrictions. The PRP-

C18 stationary phase does not experience stripping or dissolution

under the most extreme conditions. This allows for an expanded

mobile phase repertoire for use in methods development or aggres-

sive regeneration procedures.


Column: PRP-C18, 4.1 × 50 mm, 5 μm

Instrumentation: Agilent 1100 quaternary pump with UV detector

Standards: acetone, phenol, benzyl alcohol, benzene, toluene, ethyl-

benzene, propylbenzene, napthalene, pentylbenzene, hexylbenzene

Mobile phase A: 0.2% Phosphoric acid

Mobile phase B: A + 95% ACN

Gradient: 5 to 100% B in 20 min

Flow rate: 2 mL/min

Temperature: Ambient


Detection: UV at 205 nm

Conclusion

Mobile phase pH is a useful tool in methods development, particularly

for separation of neutral forms of amines or other organic bases under

alkaline conditions. Although a few more recent C18 columns boast sta-

bility in alkaline pH, column life is still considerably shorter than if used

under more favorable conditions. On the other hand, the PRP-C18 has

genuine pH and chemical stability. The stationary phase is devoid of

free silanols, does not strip, bleed, or dissolve at any pH, and therefore

can be expected to perform reliably and reproducibly throughout the

extended life of the column, regardless of mobile phase conditions.

Hamilton PRP™-C18

High-Effi ciency Separations at Any pHDerek Jensen and Mark Carrier, Hamilton Company

Hamilton Company4970 Energy Way, Reno, NV 89502

tel. (800) 648-5950, fax (775) 858-3026

Website: www.hamiltoncompany.com

Initial

After 1 M NaOH

After 1 N H2SO

4

0 10 20

Figure 1: Separation of standard mix after 200 column volumes 1 M NaOH, 1 M H2SO4.

THE APPLICATION NOTEBOOK

Call for Application Notes

LCGC is planning to publish the next issue of

Th e Application Notebook special supplement in

February. Th e publication will include vendor

application notes that describe techniques and

applications of all forms of chromatography

and capillary electrophoresis that are of im-

mediate interest to users in industry, academia,

and government. If your company is interested

in participating in these special supplements,

contact:

Edward Fantuzzi, Publisher,

(732) 346-3015

Brianne Molnar, Sales Manager,

(732) 346-3034

Stephanie Shaff er, Sales Manager,

(774) 249-1890

Application Note Preparation

It is important that each company’s mate-

rial fi t within the allotted space. Th e editors

cannot be responsible for substantial editing

or handling of application notes that deviate

from the following guidelines:

Each application note page should be no more

than 500 words in length and should follow

the following format:

• Title: short, specifi c, and clear

• Abstract: brief, one- or two-

sentence abstract

• Introduction

• Experimental Conditions

• Results

• Conclusions

• References

• Two graphic elements: one is the company

logo; the other may be a sample chromato-

gram, fi gure, or table

• Th e company’s full mailing address,

telephone number, fax number,

and Internet address

All text will be published in accordance with

LCGC ’s style to maintain uniformity through-

out the issue. It also will be checked for gram-

matical accuracy, although the content will not

be edited. Text should be sent in electronic for-

mat, preferably using Microsoft Word.

Figures

Refer to photographs, line drawings, and

graphs in the text using arabic numerals in

consecutive order (Figure 1, etc.). Company

logos, line drawings, graphs, and charts must

be professionally rendered and submitted as

.TIF or .EPS fi les with a minimum resolution

of 300 dpi. Lines of chromatograms must be

heavy enough to remain legible after reduc-

tion. Provide peak labels and identifi cation.

Provide fi gure captions as part of the text,

each identifi ed by its proper number and title.

If you wish to submit a fi gure or chromato-

gram, please follow the format of the sample

provided below.

Tables

Each table should be typed as part of the main

text document. Refer to tables in the text by

Roman numerals in consecutive order (Table

I, etc.). Every table and each column within

the table must have an appropriate heading.

Table number and title must be placed in a

continuous heading above the data presented.

If you wish to submit a table, please follow

the format of the sample provided below.

References

Literature citations must be indicated by ara-

bic numerals in parentheses. List cited refer-

ences at the end in the order of their appear-

ance. Use the following format for references:

(1) T.L. Einmann and C. Champaign, Science

387, 922–930 (1981).

Th e deadline for submitting application notes for the February issue of Th e Application Notebook is:

January 13, 2018

Th is opportunity is limited to advertisers in LCGC North America. For more information, contact: Ed Fantuzzi at (732) 346-3015,

Brianne Molnar at (732) 346-3034, or Stephanie Shaff er at (774) 249-1890

Table I: Factor levels used in the designs

Factor Nominal Value Lower Level (−1) Upper Level (+1)

Gradient profile 1 0 2

Column temperature (°C) 40 38 42

Buffer concentration 40 36 44

Mobile-phase buffer pH 5 4.8 5.2

Detection wavelength (nm) 446 441 451

Triethylamine (%) 0.23 0.21 0.25

Dimethylformamide 10 9.5 10.5

Figure 1: Chromatograms obtained us-ing the conditions under which the ion suppression problem was originally dis-covered. The ion suppression trace is shown on the bottom. Column: 75 mm × 4.6 mm ODS-3; mobile-phase A: 0.05% heptafl uorobutyric acid in water; mobile-phase B: 0.05% heptafl uorobutyric acid in acetonitrile; gradient: 5–30% B in 4 min. Peaks: 1 = metabolite, 2 = internal standard, 3 = parent drug.


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