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CORRECTION Open Access Correction: Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess Syazwan Saidin 1 , Muhammad Hafiznur Yunus 1 , Nor Dyana Zakaria 1 , Khairunisak Abdul Razak 2,3 , Lim Boon Huat 4 , Nurulhasanah Othman 1 and Rahmah Noordin 1* Correction After publication of this article [1], it was brought to our attention that we had failed to make it clear that Figures one (Figure 1 here), two (Figure 2 here) and three (Figure 3 here) were put together by grouping elements from different images. The revised figures, in which this grouping has been made explicit in the arrangement, and figure legends, in which the adjustments are clearly stated, are shown below. We regret any inconvenience that this inaccuracy in the figures in the original manuscript might have caused. Author details 1 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang 11800, Malaysia. 2 School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia. 3 NanoBiotechnology Research and Innovation (NanoBRI) Institute for Research in Molecular Medicine Universiti Sains Malaysia, Penang 11800, Malaysia. 4 School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia. Received: 4 September 2014 Accepted: 9 September 2014 Published: 10 November 2014 Reference 1. Saidin S, Yunus MH, Zakaria ND, Razak KA, Huat LB, Othman N, Noordin R: Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect Dis 2014, 14:182. doi:10.1186/1471-2334-14-533 Cite this article as: Saidin et al.: Correction: Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infectious Diseases 2014 14:533. Submit your next manuscript to BioMed Central and take full advantage of: Convenient online submission Thorough peer review No space constraints or color figure charges Immediate publication on acceptance Inclusion in PubMed, CAS, Scopus and Google Scholar Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit * Correspondence: [email protected] 1 Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang 11800, Malaysia Full list of author information is available at the end of the article © 2014 Saidin et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Saidin et al. BMC Infectious Diseases 2014, 14:533 http://www.biomedcentral.com/1471-2334/14/533

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  • CORRECTION Open Access

    Correction: Production of recombinantEntamoeba histolytica pyruvate phosphatedikinase and its application in a lateral flowdipstick test for amoebic liver abscessSyazwan Saidin1, Muhammad Hafiznur Yunus1, Nor Dyana Zakaria1, Khairunisak Abdul Razak2,3, Lim Boon Huat4,Nurulhasanah Othman1 and Rahmah Noordin1*

    CorrectionAfter publication of this article [1], it was brought to ourattention that we had failed to make it clear that Figuresone (Figure 1 here), two (Figure 2 here) and three(Figure 3 here) were put together by grouping elementsfrom different images. The revised figures, in which thisgrouping has been made explicit in the arrangement,and figure legends, in which the adjustments are clearlystated, are shown below.We regret any inconvenience that this inaccuracy in

    the figures in the original manuscript might havecaused.

    Author details1Institute for Research in Molecular Medicine, Universiti Sains Malaysia,Penang 11800, Malaysia. 2School of Materials and Mineral ResourcesEngineering, Engineering Campus, Universiti Sains Malaysia, 14300 NibongTebal, Penang, Malaysia. 3NanoBiotechnology Research and Innovation(NanoBRI) Institute for Research in Molecular Medicine Universiti SainsMalaysia, Penang 11800, Malaysia. 4School of Health Sciences, Universiti SainsMalaysia, Kubang Kerian, Kelantan 16150, Malaysia.

    Received: 4 September 2014 Accepted: 9 September 2014Published: 10 November 2014

    Reference1. Saidin S, Yunus MH, Zakaria ND, Razak KA, Huat LB, Othman N, Noordin R:

    Production of recombinant Entamoeba histolytica pyruvate phosphatedikinase and its application in a lateral flow dipstick test for amoebicliver abscess. BMC Infect Dis 2014, 14:182.

    doi:10.1186/1471-2334-14-533Cite this article as: Saidin et al.: Correction: Production of recombinantEntamoeba histolytica pyruvate phosphate dikinase and its applicationin a lateral flow dipstick test for amoebic liver abscess. BMC InfectiousDiseases 2014 14:533.

    Submit your next manuscript to BioMed Centraland take full advantage of:

    • Convenient online submission

    • Thorough peer review

    • No space constraints or color figure charges

    • Immediate publication on acceptance

    • Inclusion in PubMed, CAS, Scopus and Google Scholar

    • Research which is freely available for redistribution

    Submit your manuscript at www.biomedcentral.com/submit

    * Correspondence: [email protected] for Research in Molecular Medicine, Universiti Sains Malaysia,Penang 11800, MalaysiaFull list of author information is available at the end of the article

    © 2014 Saidin et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, andreproduction in any medium, provided the original work is properly credited. The Creative Commons Public DomainDedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article,unless otherwise stated.

    Saidin et al. BMC Infectious Diseases 2014, 14:533http://www.biomedcentral.com/1471-2334/14/533

  • Image 1AImage 1B

    Figure 1 SDS-PAGE analysis shows the purified rPPDK. Lane 1: Precision Plus Protein™ Unstained Standard Marker (Bio-Rad, USA); Lane 2:Non-induced cell from pET28a/PPDK; Lane 3: Induced cell from pET28a/PPDK; Lanes 4–5: purified rPPDK protein from elution buffer (250 mMimidazole). Arrow indicates the recombinant PPDK protein (~98 kDa). Figure one (Figure 1 here) was made by grouping elements from the ori-ginal Images 1A and 1B as follows: Lanes 1, 2 and 3 in Figure one (Figure 1 here) came from lanes M, 6 and 8 respectively in Image 1A; whileLanes 4 and 5 in Figure one (Figure 1 here) came from lanes 7 and 8 respectively in Image 1B.

    Saidin et al. BMC Infectious Diseases 2014, 14:533 Page 2 of 4http://www.biomedcentral.com/1471-2334/14/533

  • Image 1A

    Figure 2 SDS-PAGE analysis shows the purified rGal/GalNAc lectin. Lane 1: Precision Plus Protein™ Unstained Standard Marker (Bio-Rad, USA);Lane 2: Non-induced cell from pET28a/Gal-GalNAc lectin; Lane 3: Induced cell from pET28a/Gal-GalNAc lectin; Lanes 4–5: purified rGal/GalNAc lec-tin protein from elution buffer (250 mM imidazole). Arrow indicates the recombinant Gal/GalNAc lectin protein (~53 kDa). Figure two (Figure 2here) was made by grouping elements from the original image 1A as follows: Lanes 1, 2, 3, 4, and 5 in Figure two (Figure 2 here) came fromlanes M, 1, 2, 3 and 4 respectively in Image 1A.

    Saidin et al. BMC Infectious Diseases 2014, 14:533 Page 3 of 4http://www.biomedcentral.com/1471-2334/14/533

  • Image 6A

    Image 6B

    Figure 3 Representative rPPDK-LFD strips tested with serum samples. Lanes 1–3: Lateral flow test strips tested with serum samples frompatients with ALA; Lane 4: strip tested with serum from healthy individual; Lanes 5–6: strips tested with serum samples from other diseases (i.e.malaria and pyogenic liver abscess); C: control line; T: test line. Figure six (Figure 3) was made by grouping elements from original Images 6A and6B as follows: Dipstick 1 and 2 in Figure six (Figure 3) came from dipsticks 3 and 4 in Image 6A; dipstick 3 in Figure six (Figure 3) came fromdipstick 4 in Image 6B; dipsticks 4, 5 and 6 in Figure six (Figure 3) came from dipsticks 5, 6 and 7 respectively in Image 6A.

    Saidin et al. BMC Infectious Diseases 2014, 14:533 Page 4 of 4http://www.biomedcentral.com/1471-2334/14/533

    CorrectionAuthor detailsReference