coularray multi-electrode array · pdf filecoularray multi-electrode array detector ... of...

CoulArray Multi-ElectrodeArray Detector
Coularray brochure RevFfinal:Copy of 52073_MrAssociates.qxd 12/23/09 11:25 AM Page 3

The CoulArray Detector:Beyond Conventional HPLC detection
The CoulArray is a revolutionary instrument thattakes you beyond conventional HPLC-baseddetection. Whether you are engaged in leading edgeresearch, high volume assays, or other demandingtasks, the CoulArray will help you answer youranalytical problems.
The CoulArray is used worldwide by laboratories whereease of use, speed and accuracy are paramount. Thiscutting-edge technology goes far beyond the limitationsof common HPLC detectors to deliver the ultimate inperformance.
Enhance your productivity and guarantee high quality results Achieve sensitivity to femtomole levels Eliminate interferences and greatly simplify sample preparation
The Coulometric Array: A 3-D View
The CoulArray provides a 3-dimensional look at yoursample. It is the ultimate in selectivity. More than justmultiple sensors functioning independently, the sensorswork in concert to yield qualitative data based on theinherent electrochemical behavior of the analytes.
By applying increasingly higher potentials to the array ofsensors, compounds that differ in redox potential areresolved regardless of their chromatographic behavior.
This unique technology:
produces qualitative information for compound identification resolves co-eluting peaks determines peak purity simplifies sample preparation measures many analytes per sample
Software That Gives You Results
The CoulArray for Windows software integrates thedetector and HPLC components. It controls ESA pumps,autosamplers, sets electrode potentials, collects, andprocesses data. Automatic gain ranging and a uniquepatented baseline correction algorithm overcome thelimitations of other electrochemical detectors in theuse of gradient chromatography.
The power of coulometric array technology is nowexpanded with easy data transport to pattern recognitionsoftware.
The principle of CoulometricArray Technology: CompoundA is completely reacted atSensor 1 thus eliminating itfrom detection at Sensor 2.Compound B passes Sensor1 unchanged and is detectedat Sensor 2. The result isthat A and B are resolvedelectrochemically.
Flow ChannelCounter and Reference Electrodes
Working Electrodes
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B
Ba
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ESAs pioneering work in
electrochemical detection for HPLC
ensures that multi-sensor technology
is the most reliable solution to your
analytical problems.
The CoulArray detector uses a
patented coulometric sensor that
overcomes the problems associated
with older, unreliable electrode
designs. Unlike amperometric
electrodes that typically react
less than 10% of the analyte, the
coulometric sensor measures 100%.
And that means you get greater
sensitivity and selectivity.
ESA CoulArray System Go Beyond
basedDetection
HPLC or LC-MSConventional

Unsurpassed Power to Solve Your Problems
The CoulArray Systems inherent selectivity and sensitivitymake it the technology of choice for applications acrossa variety of disciplines.
Pharmaceuticals From drug discovery to quality control,the unique abilities of the CoulArray let you study drugmetabolism, assess compound stability and measureminute quantities of drugs and their metabolites in complexbiological samples.
Metabolomics Identify and measurethe effects of small perturbations usingin-vitro or in-vivo biological systems.Rapidly correlate normal, diseaseand treated patterns of molecules.
Redox Biochemistry Measure theunderlying mechanisms of reactive oxygen and nitrogenspecies processes. Study markers such as the guanineadducts, 3-nitrotyrosine and 3-chlorotyrosine, or theprotective mechanisms of vitamins, plant flavonoidsand phenolics.
Neuroscience Whether you are measuringneurotransmitters, drugs, pharmacological effects in normaland disease states, or studying the neurodegenerative effectsof oxidative stress, no better solution exists.
Food and Beverage Profile the characteristic qualitiesof products, determine integrity, identify adulteration andeven evaluate competitors products.
Clinical Diagnostics the industry standard for the analysisof catecholamine metabolites in demanding, high volumelaboratories. Reduce sample preparation, save time andmoney, while improving service. FDA approved methodsfor urine metanephrines and urine acid metabolites.
BioAnalysis enhance the performance of your LC-MS.Improve ionization performance; synthesize metaboliteson-line.
in-vitro in-vivo

Easy to Use Manipulate graphics to viewchromatograms from different perspectives and facilitateinterpretation. Create customized reports or use suppliedtemplates. Easily export the information to spreadsheetsand LIMS systems in ANDI and proprietary formats.Advanced metafile graphic capabilities make presentationsand reports simple.
Advanced capabilities ensure that you will efficiently andeasily get the results you need.
The qualitative data of PDA with 1,000 times greater sensitivity Limits of detection of fluorescence without derivatization The widest dynamic response range possiblemeasuringfemtomole to micromole levels in the same sample
The only electrochemical system compatible with gradientchromatography
Easy to integrate with LC-MS
This electrochemically-inert pump providesvirtually pulse-free solvent flow in the range of50 L/minute to 9.9 mL/minute. Electronicdiagnostics, as well as direct serial controlfrom the ESA software, make this the idealpump for a CoulArray System.
thatComponents
EnhancePerformance
Solvent Delivery Module

The temperature controlled CoulArray Organizerprovides the optimum environment to enhanceretention time reproducibility. The Organizer is aconvenient and secure location for the sensors,columns and is designed for the mounting of amanual injector, pulse dampener and gradient mixer.The CoulArray Organizer is also available in anon-thermal option.
The Autosampler is a low volume extremelyversatile multi-function injector with a unique doubleneedle, bio-inert sampling system. Designed for avariety of microtiter plate or vial sizes. Three injectionmodes provide excellent reproducibility and nosample loss with limited sample volumes. Automatedderivatization and diluting capabilities provide flexibleformats for sample preparation. Optional Features:Peltier sample tray cooling or automatic plate feeder.
The electronics module and software control from4 to 16 sensors (in increments of 4) in theWindows environment, and are easily upgradeable.All electronic connections are conveniently locatedon the front of the module for easy installation andmaintenance. Sensor potentials can be set from-1000 mV to +2000 mV in 1 mV steps. Thesoftware controls ESA pumps in either isocraticor high pressure binary gradient configurations.CoulArray Organizer
Autosamplers
CoulArray Electronics Module and Software

ESA: Pioneers in Analytical Technology for 40 years. ESA has been a pioneer in the manufacture, sale,training and support of innovative analytical instruments. With many U.S. and international patents, we are recognizedworldwide for the sensitivity, quality and performance of our instrumentation.
As a science-driven company, our primary objective is to provide instruments and support to help you answer yourtoughest analytical problemsfrom advanced research to routine analysis. Our application solutions, instrumentationand analytical services anticipate and fulfill your needs, simplify complex analyses, and accelerate research.
Combine the most reliable,
sensitive and powerful detector
commercially available today
with components optimized for
electrochemistry and get a
completely integrated system.
Whether for routine or complex
analyses, ESAs CoulArray
System is easy to learn, easy
to use and provides the
information you need to
solve your toughest analytical
problems.

70-1778Rev F
Number of Electrodes: 4, 8, 12, or 16 coulometric electrodes in series
Potential Range: independent potential control for each electrode from-1 volt to +2 volts in 1 mV increments, accurate to 2 mV
Current Ranges: 50 nA, 5 A, 100 A autoranged; full scale for eachelectrode and displayed from 10 pA to 100 A
Acquisition Rate: 2 or 10 Hz, selectable
Autozero: up to 5 A on the 50 nA and 5 A scale and 100 A on the100 A scale
Output Noise: < 5 pA peak to peak (10 M, 2 F, low filter setting)
Output Resolution: 30 fA on 50 nA gain range; 3 pA on 5 A gain range and47 pA on 100 A gain range
Power Requirements: 100/120/230/240 VAC, 50/60 Hz
Output Contact Closures: 8, programmable via software
External Start Inputs:

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