coulter lh 700 series

7/26/2019 Coulter LH 700 Series

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PN A86683AA (October 2009)Miami Education Center

COULTERLH 700 SERIES

HEMATOLOGY ANALYZER

with optional

LH SLIDEMAKER AND LH SLIDESTAINER

EASY REFERENCE GUIDE


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Safety NoticeRead all product manuals and consult with Beckman Coulter-trained personnel before attempting to operateinstrument. Do not attempt to perform any procedure before carefully reading all instructions. Always followproduct labeling and manufacturers recommendations. If in doubt as to how to proceed in any situation,contact your Beckman Coulter representative. Beckman Coulter, Inc. urges its customers to comply with allnational health and safety standards such as the use of barrier protection. This may include, but is not limited

to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any otherautomated laboratory analyzer.

Alerts for Warning and CautionThroughout this manual, you will see the appearance of these alerts for Warning and Caution conditions:

WARNINGWARNING indicates a potentially hazardous situation, which, if not avoided, could result in death or seriousinjury. May be used to indicate the possibility of erroneous data that could result in an incorrect diagnosis.

CAUTIONCAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderateinjury. It may also be used to alert against unsafe practices. May be used to indicate the possibility oferroneous data that could result in an incorrect diagnosis.

Safety Precautions

WARNINGRisk of operator injury if:

All doors covers and panels are not closed and secured in place prior to and during instrumentoperation.

The integrity of safety interlocks and sensors is compromised. Instrument alarms and error messages are not acknowledged and acted upon. You contact moving parts. You mishandle broken parts. Doors, covers and panels are not opened, closed, removed and/or replaced with care. Improper tools are used for troubleshooting.

To avoid injury: Keep doors, covers and panels closed and secured in place while the instrument is in use. Take full advantage of the safety features of the instrument.

Acknowledge and act upon instrument alarms and error messages. Keep away from moving parts. Report any broken parts to your Beckman Coulter Representative. Open/remove and close/replace doors, covers and panels with care. Use the proper tools when troubleshooting.

CAUTIONSystem integrity could be compromised and operational failures could occur if:

This equipment is used in a manner other than specified. Operate the instrument as instructed inthe product manuals.

You introduce software that is not authorized by Beckman Coulter into your computer. Onlyoperate your systems software with software authorized by Beckman Coulter.

You install software that is not an original copyrighted version. Only use software that is anoriginal copyrighted version to prevent virus contamination.

CAUTIONIf you purchased this product from anyone other than Beckman Coulter or an authorized BeckmanCoulter distributor, and, it is not presently under a Beckman Coulter service maintenance agreement,Beckman Coulter cannot guarantee that the product is fitted with the most current mandatoryengineering revisions or that you will receive the most current information bulletins concerning theproduct. If you purchased this product from a third party and would like further informationconcerning this topic, call your Beckman Coulter Representative.

This document is not intended to replace the information in your instrument Instructions forUse manual. Information in the Instructions for Use manual supersedes information in anyother manual.


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TRADEMARKS

5C, COULTER, COULTER COUNTER, FACULTY, ISOTON, LIN-C, LYSE S, and S-CAL are

trademarks of Beckman Coulter, Inc.

All other trademarks, service marks, products or services are trademarks or registered trademarks

of their respective holders.

CopyrightBeckman Coulter, Inc. 2007

All Rights Reserved


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REVISION STATUS

Revision AA Initial Issue (October 2009)

Combine the LH 750 PNA48907and LH 780 PNA36293Easy Reference Guides.

Software 2D1/2B1 or higher

2009 Beckman Coulter, Inc.


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TABLE OF CONTENTS

Hematopoiesis: The Original Family TreeNormal Histograms LH 780 and LH 750Normal Data Plots (2D and 3D)

Workstation

Log Off/Log On LH WorkstationThe Command CenterThe Eight LH Application ButtonsSpecial Help IconsLH SlideStainer IconsLH Workstation (computer) Shutdown

Daily Functions

LH 700 SeriesAnalyzer CRT and Numeric KeypadShutdown Cycle SummaryStartup Cycle SummaryRunning Latron SummaryRunning COULTER5Cand Retic-C Cell Controls Summary

LH SlideMakerSM Keypad Display Map

Suggested Daily Setup for LH SlideMakerLH SlideStainer

Suggested Daily Setup for LH SlideMaker with LH SlideStainer

Calibration

Reproducibility and Carryover

Host/LIS Down

Setting Up the LH WorkstationCreating the ToDo List

IQAP/eIQAP Information

General InformationIQAP Download ProcedureHow to Register and Enroll in eIQAP


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How to Access eIQAPUpload File Messages

Parts Diagrams

LH 700 Series

Diluter Front PanelsDiluter Front Cover (top view)

LH SlideMakerSample Access and Reservoir ModuleDispense ModuleDispense Probe Mechanism and Rinse BlockSlide Transport Module

Common Troubleshooting Fixes

Common Orderable PartsLH 700 SeriesInstrument Power Off/OnLow Vacuum Low MessageLow Vacuum Drifted MessageFluid LeaksLevel Sense MessageBackwash Tank Not Full MessageProbe MessagesRocker Bed Messages

Aspiration MessagesNo Diff ResultsNo Retic Results

LH SlideMakerDispense Probe MessagesLow Vacuum 1 or 2 MessagesShuttle or Truck Vacuum Messages

LH SlideStainerBath Not Draining Messages

Bath Not Filling MessagesAs Needed Cleaning Procedures

LH 700 SeriesLH SlideMakerLH SlideStainer

Optimizing Stain Protocols


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Hematopoiesis: The Original Family Tree

Courtesy Johns Hopkins University Press,

The Johns Hopkins Atlas of Human Functional Anatomy,Leon Schlossberg, illustrator


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Normal Histograms LH 780

RBC Histogram

Platelet Histogram

WBC Histogram

Range IndicatorsUpper and lower limitsdefined by the lab or

can be turned off.

Normally has two curves.Green curve is the smoothor raw data. Black curve is

the fitted data.

Black line indicates the35 fL lower threshold.Area checked for

presence of interference.


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Normal Histograms LH 750

RBC Histogram

Platelet Histogram

WBC Histogram

Normally has two curves.Green curve is the smoothor raw data. Black curve isthe fitted data.

Black line indicates the

35 fL lower threshold.Area checked for

resence of interference.


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Normal Data Plots

2D Data Plot (WBC Differential)

2D Data Plot (WBC Differential) (Lymphs removed)

Monocytes

Lymphocytes

Non-white cells

Neutrophils

Eosinophils

Basophils

Basophils


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3D Data Plots (WBC Differential)

3D Data Plot (WBC Differential) (default view)

3D Data Plot (WBC Differential) (rotated 90 degrees)

Point of origin(0, 0, 0) xyzcoordinate

y-axisVolume

z-axisConductivity

x-axis

Light Scatter



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2D and 3D Data Plots (Reticulocytes)

2D Data Plot (Reticulocytes)

3D Data Plot (Reticulocytes)

Mature RBCs

Platelets/Non-red cells

Reticulocytes

WBCs



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Log Off/Log On LH Workstation

To Log Off the LH Workstation

Step Action

1 Select from the Command Center. The Log Off the System windowappears.

The default selection is Log Off Current User.

2Select to confirm that you want to log off the current user name.

To Log On to the LH Workstation

Step Action

Type your user name or verify the current name displayed.1

User names are defined by those with Lab Admin (Level 3) access.

Type your password.

2 Remember that passwords are case-sensitive.

If you forget your password, the Lab Admin access can reset it for you.

3Select at the bottom of the logon screen. The Workstation checks your

password and starts the LH Workstation.


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The Command Center

The Command Center is the green bar located across the bottomof the desktop area.

When you want to change the area you are viewing, just select anew button from the Command Center. The program automatically

closes what was open and then opens the new selection.

There are three exceptions:

If the Run Configuration screen is open, you exit by selecting

either (OK, save and close) or the red to cancel any changes

you made.

If the Helpscreen is open, you exit by selecting the small x in the upper

right corner of the Help screen.

If the History Logsare open, exit by selecting the small x in the upperright corner of the History Logs screen.

The user currentlylogged on.

Body Fluid Option

Eight LHApplication buttons

Status Indicatorsfor Analyzer orSlideMaker and

PC

Barcode entryScanned or Typed

Predilute Optionwith

dilution factor entry

Process TypeUsually has AutoAnalysis selected.

Default TypeSelects the sample testmode if no barcode or

Random Access

Enable/DisableRandom Access

Traffic-light iconInstrument Status


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The Eight LH Application Buttons

Run Configuration

Takes you to the screen to view/change Print, LIS and LHSlideMaker run choices. Also access to AutoStop choices.

Patient Results

Takes you to the screen to view patient results from either theResults/Graphics view or the Database/ToDo view.

Quality Assurance

Takes you to the screens associated with various QA data:

Daily Checks, Quality Control, Extended QC, XB, XM,Reproducibility, Carryover, Calibration.

Help

Provides access to an extensive multimedia help system builtinto the software.

History Logs Viewer

Provides access to the various electronic logs for review, toadd comments, print or archive.

System Status

View current details about the system , such as softwarerevision.

System Setup

Provides access to the various setup functions to customizeyour workflow: General Settings, Quality Assurance, Patientetc.

Log Off (Exit) the System

Choose between Log off current (default) or Shutdown theWorkstation.


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Special HELP Icons

These icons are found within the HELP system on the Workstation.

Help button on Main Command Centeror a screens tool bar.

Help Mode(Context Sensitive Help)button.

In a HELP topic, shows that the topic

was changed in the current revision ofthe software and HELP.

Shows that you can find a list of topicsrelatedto the current HELP topic on thescreen.

Indicates that you can find a sub-procedure with a graphicby clicking onthe Pictureicon.

Indicates that you can go to a video clipof maintenance or other procedure byclicking on the Projectoricon.


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COULTER

LHSlideStainerIcons

From System Set Up, go to .Select tab.

Save Protocol

Saves newly created or modified staining protocol.

Delete Protocol

Deletes selected protocol. You cannot delete a protocolwhile it is in use.

Download SlideStainer Protocol (LabAdmin only)

Downloads a saved or modifiedstain protocol to theSlideStainer. The icon will only be available when the

SlideStainer is in Standby mode.New Protocol

Provides blank Staining Protocol data fields to prepareto set up a new protocol or to modify an existingprotocol.

Open SlideStainer Arm Dialog Box (LabAdmin only)

Arm is aligned in response to error situations.

SlideStainer Status

Provides information about Serial # and cycle countamong others. Buttons to open and close gripper andreinitialize arm.

SlideStainer Input Queue Status (LabAdmin only)

Displays SlideStainer input/output queue configurationfor configuring the parking lot / empty basket areas.

Drain SlideStainer Reagent Lines

In preparation for shutting down SlideStainer orpreventing spillage during maintenance procedures.

Monitor Sensor Status

To troubleshoot error messages. Also monitor valuesfor temperatures, pressure sensors, and battery VDCinformation.

Download SlideStainer Software (LabAdmin only)

To upgrade software or respond to error situations


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LHWorkstation (computer) Shutdown

The LH Workstation (computer) should go through this procedure at least once per week.

You may have to shut down and restart the Workstation in response to certain error

situations or if the computer doesnt respond. Be sure to read any Help message for error

recovery carefully for this indication.

In the event of a communication problem between the Workstation and the LH 780

Analyzer, you may need to reset the Analyzer using the RESETbutton on the upper right

corner of the Analyzer cover as well as selecting to shut down and restart the Workstation.

Step Action

1Select from the Command Center.

2 Select Shutdown the Workstation, then Select OK

The Workstation will save any unsaved data, close all

applications and display a window notifying you it is shuttingdown the workstation. This may take a minute.

Next, a window is displayed that states you may now power

off the workstation.

3

Turn off the computer workstation power using the power

button on the front of the computer tower.

Always verify that the power actually went off. Sometimes,you need to hold in the button until all power is off.

4 After one minute, turn the power back on.

5Log on to the workstation when the Log On window

reappears.


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Analyzer CRT and Numeric Keypad

LH 780

LCD Display

Numeric Keypad

Always return to this

screen to run theinstrument.

Use this button toaccess the Main Menu


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Analyzer CRT and Numeric Keypad

LH 750

LCD Display

Numeric Keypad

Always return to thisscreen to run the

instrument.

Use this button to

access the Main Menu


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Shutdown Cycle Summary

Shutdown (Cleaning Cycle)

Step Action

1Verify pneumatics are On. (Look at the Power Supplydisplay. If off, press the key on the Analyzer NumericKeypad.)

2aIf you have an LH SlideMaker, select Shutdown from theSlideMaker keypad menu. Wait until cycle completesbefore proceeding to next step.

2b

When the Analyzer CRT and the LCD display both

indicate Ready, press the key on the AnalyzerNumeric Keypad. This pulls the enzymatic cleaning agentinto the Diluter.

Shutdown must be performed once every 24 hoursthat the instrument is in use.

The cleaning agent must remain in the Diluter for aminimum of 30 minutes.

Note Cleaning agent removal cycles are performedautomatically at the end of the programmed cleaning

time. (30 minutes to 24 hours)No Background Checks or Electronic Tests areperformed at this time.

3 Proceed to Startup Summary


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Startup Cycle Summary

Startup (Daily Checks)

Step Action

1 Press the key on the Analyzer Numeric Keypad.

After the cleaning agent removal cycles, this is theonly key you can use.

The instrument automatically performs the followingchecks:

Background counts (CBC, DIFF, RETIC)Ramp & Precision electronic checksVarious voltage, pressure and temperature checks

Note If you have an LH SlideMaker, it will startup automaticallyif it was in Shutdown. If not, run Startup from the SMkeypad.

2

Review the Daily Checks results:Look at the Workstation Daily Checks screen(clickon the Command Center, then click from thecommon toolbar) or look at the automatic printout of

results.If any background or main system checks show FAILthen repeat the required test from the Analyzer CRTscreen:

Select Main Menu,Analyzer Functions, StartupTests.

As required select Backgroundor RamporPrecision.


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Running Latron Summary

COULTERLATRON Primer and ControlRun this check once every 24 hours.LATRONPrimer and Control - always run as a pair.Always run the Patron Primer first to clean the pathway through the

flowcell.

Follow with the Latron Control to check and verify the VCS gains, CVsand laser alignment.

Step Action

1

At the Workstation:

Choose Auto Analysis in the Process Type field on

the Command Center.

Open the LATRON folder on the QC screen.

2At the Analyzer Numeric Keypad, press (Diff &Retic) or (Diff). Then press .

3

Press the key on the Analyzer Numeric Keypad to runthe Primer.

Present the Primer vial to the manual aspiration tip

and remove when you hear the beep.

If the results are 500, continue with the Control.

If the results are > 500, repeat the Primer.

4

Press to continue (do not pressthe key again)

Very gently, invert the Control vial 5-8 times, beingcareful to avoid bubbles or foam.

Present the Control vial to the manual aspiration tipand remove when you hear the beep.

If any results are out of range, repeat the Primer, thenrepeat the Control.

5Press the key twiceto return to READY at the

Analyzer Numeric Keypad.


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Running 5C and Retic-C Cell Controls Summary

COULTER5CCell Control and RETIC-C Cell ControlThe frequency of running these controls depends upon

regulatory and laboratory policy for quality assuranceprograms.

Step Action

1Allow vials to equilibrate at room temperature for 10-15minutes.

Mix each vial individually, by hand, using the 8 x 8 x 8mixing method as described in the control package insert:

a)

Begin with the vial upright between the palms of your

hands and roll the full length, forward and backwardseight times. (first eight)

b)Next turn vial upside down between the palms of yourhands and roll the full length, forward and backwardseight times. (second eight)

2

c)Next hold vial in one hand and invert eight times. (thirdeight)

Note

1

Perform the above steps twicefor 5C Cell Control vials.

For RETIC-C Cell Control vials perform the above stepsonce.

3Visually check each vial after mixing to ensure cells aresuspended. If cells are not suspended, then repeat onemore set.

4Place the control vials, barcode face up, into a regularcassette and place the cassette into the loading bay.

5When analysis is complete, remove control vials from the

cassette and store them in the refrigerator.Note

2

The entire process, steps 1-5 must be completed within 30minutes to preserve control stability.

6Review control results according to your laboratoryspolicy.


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COULTERLH SlideMaker Keypad Display Map

MAIN MENU

ROUTINE FUNCTIONS*

SERVICE FUNCTIONS

SPECIAL FUNCTIONS

RUN MODE

SPECIAL CYCLES

MAIN MENU ROUTINE FUNCTIONS*

PRINTER LABELFUNCTIONS

Save Printer Label IntensityIncrement Intensity

Decrement Intensity

SYSTEM SETTINGSDisplays Software/Hardware Rev

ROUTINE FLUIDICS*

PNEUMATIC/POWERSUPPLIES

Displays Pressures, Vacuums,Voltages

ADVANCE BASKETManually advance basket oneposition

UNLOCK CASSETTEManually unlocks slide cassette

LOCK CASSETTEManually locks slide cassette

Prime ReagentReservoirs

Drain

Run STARTUP*

Backwash Tubing

Drain VacAccumulator

Run SHUTDOWN*

MAIN MENU SERVICE FUNCTIONSService Use Only needs Service Password

MAIN MENU SPECIAL FUNCTIONS

DEFINE SMEAR SPEED

DRYER TEMP

DISPLAY TEST

DRYER FUNCTION TEST

REBOOT PRINTER

SENSOR STATUS

KEYPAD TEST

MAIN MENU RUN MODE

VIEW ERROR

MAIN MENU SPECIAL CYCLESPRINT LABEL

Prints test Dummy label after replacing Labels or Ribbon

BLEACH SYSTEM

The choices in green are your daily functions.


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LH SlideMaker Suggested Daily Setup

These checks/setups may be done during the Shutdown

cycle, or at any time appropriate for your lab.

LH SlideMaker - only

Verify that the Slide Cassette is full.

Check Label and Ribbon supply, replace ifnecessary. (refer to Help)

Place up to twelve empty slide baskets in thefront and rear tracks. (if no LH SlideStainer)

Inspect dispense probe and rinse cup and cleanif any traces of blood are seen. (refer to Help)

Once the system is operating automatically, do not grabslides prematurely, this can cause jams and resets. Letthe SlideMaker do its job.


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LH SlideMaker With LH SlideStainerSuggested Daily Setup

These checks/setups may be done during the Shutdown cycle, or

at any time appropriate for your lab. Depending on workload,SlideStainer baths may need attention more than once a day.

LH SlideMaker

Verify that the Slide Cassette is full.

Check Label and Ribbon supply, replace if necessary.

Verify there are no more than six slide baskets in theSlideMaker and locate them in the rear track. Thereshould be no baskets in the front track.

Inspect dispense probe and rinse cup and clean if any

traces of blood are seen. (refer to Help)

LH SlideStainer

Place the SlideStainer in Standby state.

Place six slide baskets in the Empty Basket area.

Verify bath reagent container levels. Replace/Refill asnecessary.

Drain all baths.

Check Waste Containers. Empty if necessary.(Waste 1 = Aqueous, Waste 2 = Methanol)

Check the stain baths for excess sediment and cleanwith methanol if necessary.

Fill all baths (according to your labs protocol)

Place the SlideStainer in Autostate. Once the majorworkload finishes, empty baskets are stained if left in

Auto. You may change between Standby and Auto state.

Once the system is operating automatically, do not grabslides prematurely, this can cause jams and resets. Let theSlideMaker and SlideStainer do their jobs.

Note: Your Applications Specialist may help you to revise

these setups to better suit your labs protocols and needs.


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Calibration Summary

Note: to be used by Beckman Coulter trained personnel only

1 Shutdown for 30 minutesto ensure apertures are clean.

2Check Reagent Levels:

Diluent (1/2 to 2/3 full)

CBC Lytic Reagent ( full 1L or 5L)

3 Run Startup and verify Daily Checks results.

4 Analyze COULTER5CCell Controls.

5 Disable Automatic Print on the Run Configuration screen.

6Prepare one S-CALCalibrator tube according to the packageinsert directions.

7

Run S-CAL Calibrator:

Set up Workstation screen as described in HELP anddelete previous results from the Calibration table.

Set up S-CAL Calibrator reference values required foryour reagent system.

Remember to choose the correct S-CAL Calibrator lot #from the box on the left of the Calibration screen.

At the Analyzer CRT screen, turn Blood Detector ON, setthe # of aspirations/tube to 11, verify Default Type is C.

8

After calibration runs are complete, review the calibration dataand transmit new cal factors if necessary:

Prepare Analyzer to RECEIVE new values:

From the Analyzer CRT screen, select the following:Main Menu, Analyzer Functions, Calibration,Receive Average Cal Factors.

SelectAdjust Calibrationbutton on the specific toolbarfrom the Workstation calibration run screen.

9When finished, be sure to return the Workstation and AnalyzerCRT screen to your labs default settings.

10If any calibration factor changes were made, rerun 5C Cell controls toverify the calibration.


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Reproducibility and Carryover Summary

Note: to be used by Beckman Coulter trained personnel only

Disable Automatic Print on the Run Configuration screen.

1Run Reproducibility(Default Type set to C or CD) with the selected normalwhole blood specimen. Refer to HELP for sample requirements.

aSet up the Workstation screen as described in HELP and deleteprevious results from the table.

bSet the # aspirations/tube to 1 and cycle a separate sample collectedin a salt of EDTA.

cUsing 2-6 tubes from the same donor (5 mL EDTA), pool, mix andaliquot the sample into 2-6 clean EDTA tubes.

d Set the # of aspirations/tube to 5 on the Analyzer CRT screen.

e Place 2 of the tubes in the same cassette in the right loading bay.

f After the samples are analyzed, review the results.

Note 1The procedure begins with an N=10. If all parameters pass, you maystop processing. Otherwise, you may need more data. See Note 2below.

gVerify that the %CV (Coefficient of Variation) does not exceed theestablished limits.

Note 2

Reproducibility Limits for CBC

Parameter %CV

WBC 1.7

RBC 0.8

Hgb 0.8

MCV 0.8

Plt 3.3

MPV 2.2

If any limit is exceeded, repeat with two more tubes of the prepared sampleblood, until at least 11 but not more than 31 whole blood samples havebeen analyzed. You may stop the analysis at any point between 11 and 31runs if the reproducibility established limits have been met. You may notpick and choose which runs are included. Runs included for theassessment need to be consecutive runs.

If any instrument results exceed these limits, repeat with another donorsample. If you still have a failure, an instrument problem may exist. Callyour Beckman Coulter Representative. (Note: the above limits are alsoavailable in HELP)


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2Run Carryover study(Default Type set to C) with the selected bloodsamples and 3 separate diluent tubes.

aSet up Workstation screen as described in HELP and deleteprevious results from the table.

b

Set # aspirations/tube to 1 and turn the Blood Detector OFFat

theAnalyzer CRT screen.

c Run the samples and diluent tubes.

d Check results.


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What To Do If Your Host/LIS Goes Down

Setting up the LH Workstation

1

At the LH Workstation, from the Command Center, go to:

(System Set Up)

(General Settings)

2 Select Cass/Pos or Sample ID as applicable to your situation:

aSelect Cass/Pos as the only Positive Identifier if you do not havebar code labels.

bSelect Sample ID as the only Positive Identifier if you are using bar

code labels.

3 Click on (OK) to save.

4 Go to (Patient Results) (Database / ToDo List)

5 On the Common Toolbar, select (Add Sample Requests)

NOTE

Before adding entries on the ToDo List, do you want to set up theCass/Pos # to Autosequence?This makes preassigning more efficient.

IfYES, go to step 6.

If NO, go to Processing Preassigned Samples.6 On the Add Sample Requestswindow, select (AutoSequence)

NOTESelect HELPfrom the Setting Up AutoSequencingwindow to learn moreabout available options.

7 Set AutoSequence Control At Test Level.

8 Select to autosequence only Cass/Pos for the test modes you are running.

9 Enter the starting Cass/Pos number for each test mode.

10 Select (OK) to continue.


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What To Do If Your Host/LIS Goes Down

Creating the ToDo List

1On the Add Sample Requestwindow, select Diff (for CBC/Diff), CBC(for CBC only), for example.

Tubes without bar code labels Tubes with bar code labels

2

If you saidYes toAutosequencing of Cass/Pos #,you should see the firstCass/Pos # in the Cass/Posfield.

2

Place the cursor in the Sample IDfield.

3

If you said No toAutosequencing of Cass/Pos#,type the first Cass/Pos# into theCass/Pos field.

3

Use the hand-held bar codescanner to scan the Sample IDin the Sample ID field. Verify IDscanned.

4 Use the key to move the cursor to the Patient ID field.Patient ID is the unique identifier for each sample. You must assign aPatient IDwhen you create a ToDo List. This can be any number that isunique to the patient, e.g., accession #, Medical Records #, Social Security#.

5[Optional] You may enter additional demographic information, includingPatient name, Age, Gender, Physician and/or Location.

6 Select to save an entry and make additional entries on the ToDo List.

7 After making your final entry, select again.

8 Select (Close) to exit the Add Sample Requestwindow.

9Go to the ToDo List CBC/Diff (or other Test Mode) to verify that yourentries are correct.

NOTEIf the entries are not immediately visible, you may need to double click onthe folder for the appropriate Test Mode to refresh the screen.

10 Remember to check the following!

In (Run Configuration)

Select options according to your lab protocol

While the Host/LIS is down, you may want to disableit. Remember

to change your settings when the Host/LIS comes back on-line.Select (SlideMaker) options, if necessary.

Select (OK) to save.

NOTE

It is also possible to print two sample reports during a down time, oneChartable and one Laboratory. Go to System Setup>>General>>ReportingOptions>>Printout Options. Save.Then go to Run Configurationand set print to All Samples. Save.


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IQAP/eIQAP General Information

Important Points to Remember When Setting Up eIQAP

Make note of the following:Instrument IQAP Participant # Serial Number System ID

Number to Call for Help: 800-526-7694 (USA and Canada)


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IQAP Download Procedure

Step Action

1 Select on the Command Center.

2 Select to display the CONTROLS window.

3 Select . An IQAP Disk Storage window appears.

4Ensure the removable media (formatted CD, flash drive, or floppy disk) isinserted in the appropriate drive or connected to the computer.

5 Select the lot numbers you want copied to the removable media.

6 Select to begin copying the control information.

7 The "Save as" window appears.

8 Specify the location of your removable media and select "Save".

9After the download process is complete, retrieve the removable media from theappropriate drive or USB location

10Use the files downloaded to submit the data through the eIQAP website. Filesmay also be submitted via e-mail or as a last resort mailed to IQAP.


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How to Register and Enroll in eIQAPYou must be registered on the Beckman Coulter website to gain access to the ElectronicQuality Assurance Programs. In order to access the Hematology eIQAP program, youneed to set up your account (i.e. obtain your instruments IQAP number).

How to Access eIQAP

Step Action

1 Go towww.beckmancoulter.com

2 Hover over the words Customer Support on the menu bar.

3 From the drop down box choose QA & Service Programs

4 Select the link Enroll in IQAP near the middle of the page

5On the Quality Assurance Programs window there are links to register andenroll.

NoteIf you already have a login ID and password, proceed to "Enroll in eIQAP".Skip the registration.

Step Action

1 Go towww.beckmancoulter.com.

2 Hover over the words Customer Support on the menu bar.

3 From the drop-down box choose QA & Service Programs.

4 Select the link Go to eIQAP near the middle of the page.

5On the Quality Assurance Programs window, select the link Go to

Hematology eIQAP..

6 On the next window enter your Beckman Coulter username and password.

7On the following page look for Hematology Resources and select eIQAPHelp. This begins a breeze presentation designed to walk you through thefunctionality provided in the Hematology eIQAP application.


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Upload File Messages

Message Interpretation

Upload successful The files have been successfully uploaded.

ID on diskettedoesnt matchinstruments ID

The IQAP ID on the diskette, obtained from the instrument setup,does not match the IQAP ID displayed for the selected instrument.

1. Ensure the data on the diskette is from the selectedinstrument.

2. Ensure the IQAP ID is entered correctly in the instrumentsetup.

Not matching serialnumber on diskette

The diskette serial number, obtained from the instrument setup,

does not match the serial number displayed for the selectedinstrument.

1. Ensure the data is from the selected instrument.2. Ensure the serial number is entered correctly in the

instrument setup.3. Ensure there are no spaces or symbols within the serial

number.

Drive not ready or

unreadable diskette

The drive cannot access or read the diskette.1. Ensure the diskette is inserted into drive A.

2. Repeat download and upload process with a new diskette.3. Ensure diskette drive is functional.

Windows temporaryfolder missing

The Windows operating system temporary folder is missing.

Invalid datacollection software

Coulter STKS, MAXM, or HMX data was collected with anunacceptable software version.

1. Reconfigure diskette at the Resources page.2. Repeat download and upload process with newly

configured diskette.

Not an IQAPdiskette orincompletedownload

Diskette is not configured as an IQAP diskette.1. Ensure diskette is from IQAP download.2. Ensure instrument provided a successful download

message.3. Repeat download and upload process with a new diskette.


37/71


25 of 59

Message Interpretation

Incomplete controldata file

The diskette does not contain a complete control file.1. Ensure instrument provided a successful download

message.

2. Repeat download and upload process with a new diskette.

Failed to uploadIQAP files

An interruption occurred during the transfer of data through theinternet.

1. Repeat upload process at a later time using the samediskette.

Insufficient privilege

to access filesystem

Data on the diskette cannot be accessed as the applet certificatethat is required to upload the IQAP files from a diskette was notaccepted.

1. Logout of eIQAP and out of the Beckman Coulter website.Reenter eIQAP and accept the certificate at the UploadFiles page by selecting either Yes orAlways.

2. If unable to accept the certificate, enter data manually.

Drive writeprotected

Data on the diskette cannot be accessed as the diskette is writeprotected.

1. Ensure the window in the lower left corner of the diskette isclosed.

Couldnt create files

in the floppy, pleasecheck its writableand has xxxx bytesor more free in thefloppy

The diskette does not have sufficient space to create theIQAPUPLOAD file.


Corrupted IQAP fileon MAXM, STKS, orHmXdiskette

The file has been corrupted.



38/71

PN

A86683AA(October2009)

26

of59

COULT

ER

LH700Series

Dilu

ter

Fron

tPanel


39/71

PN


27

of59

COULT

ER

LH700Series

Dilu

terFron

tCover

(topv

iew

)

SAMPLINGSTATION(A)

RAND

OMACCESSMODULE(B)

DIFFMODULE

(C)

1

NeedleAsse

mbly

4

RearBloodDetector(underandbe

hind)

16

FlowCell

2

FrontBlood

Detector

9

DiffShe

arValve(CVL85/126)

17

TripleTransdu

cer

3

BloodSamp

lingValve

10

ReticSh

earValve(CVL87/127)

18

DiffMixingCh

amber

(VC25)

5

NeedleVent

Chamber

11

StainPu

mp(PM5)

6

ManualAspirationTip

12

Stained

BloodShearValve(CVL93

/128)

7

AspirationP

ump(PM9)

13

ReticMixingChamber(VC17)

8

ProbeWipe

Assembly

14

StainMixingChamber(VC24)

15

Blood/S

tainAspirationPump(PM11)

(behind)


40/71

PN


28

of59

COULTER

LHSLIDEMAK

ER

SampleAccessandReservoir

Module

(undertheDiluterfrontcoverontheleft)


41/71

PN


29

of59

COULTER

LHSLIDEMAK

ER

DispenseModule

(intheLHS

lideMakeronthefront

right)

SOL1

SOL2

SOL3

SOL4

SOL5

SOL6

SOL7

SOL8

SOL9

SOL10

Dispense2

Manifold

card

Dispense1

Manifold

card

SOL14

SOL15

SOL16

SOL17

SOL18

SOL19

SOL20

SOL21

SOL22

SOL23

FD5

VL4B

FD3VL3B

VL10

VL14

VL15

VL16

VL17

VL18

VL19

VL20

FD

6

VL8

FD8

DP1

VL7

6985076D

DP2

VL8

DP1

VL6

NewC

onfigura

tion


42/71

PN


30

of59

COULTER

LHSLIDEMAK

ER

DispenseProb

eMechanismandRinseBlock


43/71

PN


31

of59

COULTER

LHSLIDEMAK

ER

SlideTransportModule


44/71


32 of 59

Common Troubleshooting Fixes

Common Orderable Parts

FluidicsLow Vacuum Low Message

Low Vacuum Drifted Message

Fluid Leaks

Level Sense Message

Backwash Tank Not Full Errors

Mechanical

Probe Errors

Aspiration Errors

Rockerbed Errors

Results

No Diff Results

No Retic Results

LH SlideMaker

Dispense Probe Errors

Vacuum Errors

LH SlideStainer

Bath Not Draining Errors

Bath Not Filling Errors


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33 of 59

Common Orderable Parts

Part Number Part Description Quantity P.O.6807145 Needle Pierce Cartridge Assembly 1 Ea Y

3203015 Tubing, silicone, round, 0.062 id., black stripe 10 Ft. N

3213135 Tubing, silicone, I-beam, 0.062 id., black stripe 10 Ft. N

3213163 Tubing, silicone, round, 0.062 id x .047W, brown 10 Ft. N

3203016 Tubing, silicone, round, 0.079 id, blue stripe 10 Ft. N

3213137 Tubing, silicone, I-beam, 0.079 id, blue stripe 10 Ft. N

3213176 Tubing, silicone, round, 0.079 id x .039W, yellow 10 Ft. N

6706295 Pick-up tubing, universal, 10-20L, Clenz 1 Ea Y

6706296 Pick-up tubing, for 5L Lyse S-III 1 Ea Y

6706297 Pick-up tubing, 0.5-1.9L for 1L Lyse S-III and Pak 1 Ea Y

6805636 Waste line tubing 1 Ea Y

178453 Diluent pick-up tube with float sensor, 20L 1 Ea Y

5120248 Tubing, Waste Level Sensor Assembly 1 Ea Y

1022450 Air Filer (under Power Supply) 1 Ea Y

1022868 Air Filter (rear cover Analyzer) 1 Ea Y

2517119 Screw cap for large pick-up tubes 1 Ea N

2523574 Screw cap for small pick-up tubes 1 Ea N

6232263 Y connector, .050 id x .130 od 2 Ea N

6232259 Fitting, hose barb, Y, .093 id x .093 od 2 Ea N

3202036 Tubing, polyurethane, .082 id x .144 od 14 Ft N

6855763 Pull apart std. pinch valve, single 2 Ea N

5415206 Pinch valve removal tool 1 Ea Y

6605505 Glass slides, 1 gross=2 packages SM 5-10 Gr Y

2016733 Slide Labels, 1500/roll SM 1 Roll Y

2016732 SM Printer Ribbon SM 1 Roll Y

6806470 Slide Cassette SM 1 Ea Y

6806894 Slide Basket SM 1 Ea Y

2603047 Air filter SM 1 Ea Y

1024888 Bath, Liquid ST 1 Ea Y

6807094 Reagent Rack, round opening (1 gal.) ST 1 Ea Y

6807093Reagent Rack, 2 rectangular openings(2L)

ST 1 Ea Y

1024889 Tray, bath, plastic (holds five baths) ST 1 Ea Y

1024915 Tray, divider ST 1 Ea Y

6706296 Tubing, pickup assembly, reagent ST 1 Ea Y

6806976 Filter, Liquid, Waste / Reagent, w/tubing ST 1 Ea Y

5120249Pickup tubing, Di water, level senseassy.

ST 1 Ea Y

A35473 Accuvette (200/bx) 1 Bx Y


46/71


34 of 59

Instrument Power Off/On

Power Off

Step Action

1 Press on the Analyzer Control keypad.

2 Turn off the main breaker switch on the left side of the Power Supply.

WARNINGRisk of personal injury. A shock hazard exists if the power cord isconnected. Unplug the primary power cord before performing theseprocedures.

3Disconnect the primary power cord at the source (not at the powersupply).

Power On

Step Action

1 Reconnect primary power cord.

2 Turn on the main breaker switch on the left side of the Power Supply

3 Press on the Analyzer Control keypad.

LH SlideMaker

There is a power button on the front of the LH SlideMaker to turn poweroff and on. If required by a procedure, there is a Main Power switch onthe rear of the SlideMaker.

LH SlideStainer

There is a power button on the left side of the LH SlideStainer to turnpower on and off. If required by a procedure, there is a Main Powerswitch on the rear of the SlideStainer.


47/71


35 of 59

Low Vacuum Low MessageWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.

Low

VacuumLow?

Start Yes

1. Empty/Clean jar**.

2. Ensure float is down.

3. Reattach jar without

cross-threading.

Verify Low Vacuum

reading is 6.000 .010using F92.

Adjust if necessary.

Is Vac Trap

Full?

Call BCI

Yes

YesNo

No

Done

Yes

Look at your

Vacuum Isolator

Chambers (VICs)

Yes

No

No

Are one or both

of the VICs full

of liquid?

No

Yes

Yes

Are one or both

VICs cracked?

Call BCI

Did VICs

drain?

Drain the VICs.

Press F05, then

16,

then Enter

Press Stop

three times.

Verify Low Vacuum

reading is 6.000 .010

using F92.


Are all lines

connected to top

of VICs?

Are there

holes in any of

the lines?

Reconnect

Replace the

tubing.

No

Verify Low Vacuum


using F92.


Yes Yes

**Power Off instrument

before performing

procedure.

Follow HELP.

Able toadjust?

Verify.

Run aspecimen

Verify Low Vacuum


using F92.


YesAble to

adjust?

Flush Low Vac Line

(see attached

procedure)

Able to

adjust?

Done

No

No

No

Resolved?

Yes

No


48/71


36 of 59

Check the Vacuum Overflow Tank (VC22). If

over half full stop and call BCI. If empty or less

than half full, proceed to next step.

Remove the right side panel.

Open the lower door.

Remove the clear plastic shield.

Locate the low vacuum tubing connected to the

area labeled COUNT VACUUM on the right rear

panel. Disconnect the smaller tubing from the

front of the fitting.

Dip the disconnected end of the tubing into a

container with no more than 20 mL of DI water

until it is all aspirated.

Leave the tubing disconnected for ten (10)

minutes while it draws air to dry the vacuum line.

Reconnect the tubing, replace the plastic shield,

close the door and replace the right side panel.

Locate the Vacuum Overflow Tank (VC22) and

disconnect the float sensor connector, P-13/J-

13.

Press STOP on the keypad and allow VC22 todrain.

Once VC22 is empty, reconnect P-13/J-13. (If

alarm is triggered, VACUUM OVERFLOW

TANK STILL FULL press ALARM RESET on

the keypad and disregard error message.)

Flush Low Vacuum Line

Return toLow Vac Low

flowchart


49/71


37 of 59

Low Vacuum Drifted MessageWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.

Low

VacuumDrifted?

No

Yes

Wont adjust?Sluggish adjustment?

Drifting?

Yes

Yes

Yes

All Pass &Message gone?

PerformStartup

Start

Verify Low Vacuumreading is 6.000 .010

using F92.Adjust if necessary.

Perform F92.Verify low vac

reading 6.000 .010

Call BCIDone

Check all count tubings. Verifytubing not cut through pinch

valves. Verify RBC sweepflowtubings secure.

Call BCI

Found issue withtubing?

Yes

Able toadjust?

Flush Low Vac Line(see attached

procedure)

Repair andreplace tubing.

Able to adjust?Verify Low Vacuum

reading is 6.000 .010using F92.


No

No No

PerformReset

Messagereoccurs?

Yes

No

Yes

RunSpecimen

No


50/71


38 of 59

Check the Vacuum Overflow Tank (VC22). If

over half full stop and call BCI. If empty or less

than half full, proceed to next step.

Remove the right side panel.

Open the lower door.

Remove the clear plastic shield.

Locate the low vacuum tubing connected to the

area labeled COUNT VACUUM on the right rearpanel. Disconnect the smaller tubing from the

front of the fitting.

Dip the disconnected end of the tubing into a

container with no more than 20 mL of DI water

until it is all aspirated.

Leave the tubing disconnected for ten (10)

minutes while it draws air to dry the vacuum line.

Reconnect the tubing, replace the plastic shield,

close the door and replace the right side panel.

Locate the Vacuum Overflow Tank (VC22) and

disconnect the float sensor connector, P-13/J-13.

Press STOP on the keypad and allow VC22 to

drain.

Once VC22 is empty, reconnect P-13/J-13. (If

alarm is triggered, VACUUM OVERFLOW

TANK STILL FULL press ALARM RESET on

the keypad and disregard error message.)

Flush Low Vacuum Line

Return to

Low Vac Drifted

flowchart


51/71


39 of 59

Fluid LeaksReview information in HELP and in the TS3-Replacing Components Module.Whenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.

I-beamtubing leak?

Round, red stripe

silicon in Random

Access Module?

Other

tubing leak?

**Find correct

replacementI-beam tubing with

same color stripe.

Cut new piece

of tubing tosame length as

original.

Disconnect leakytubing* and pull

out of pinch valve.

Thread new piece

of tubing carefully

through tracks in

pinch valve.

Yes

Yes

No

**Find correct

replacement

tubing with same color

stripe or part number.

Carefully

reconnect the

two ends.

Start

Done

Cut new piece

of tubing to

same length as

original.

Disconnect leaky

tubing*.

Carefully connect

new tubingDone

No

**Find correct

replacement

tubing with same

color stripe.

Cut new piece

of tubing to

same length as

original.

Yes

No

Disconnect leaky

tubing*.

Carefully connect

new tubingDone

Carefully thread

new tubing

through blue

spacer sleeve

(Fig. 1)


before performing

procedure.

Follow HELP.

Fig. 1

*Be sure to mark

the exact places

where tubing is

removed.


52/71


40 of 59

Level Sense MessageWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.

Start

Done

Call BCI

Is

container

empty?

Yes

Change container

and update

reagent log.

No

Verify all tubing

connections from

reagent toinstrument.

If diluent, check float

sensor in container

and sensorconnection (Fig. 1).

Any tubing

disconnected/

pinched?

No

Yes

Reconnect/

straightentubing

Reagent/

Level out

message

Press Startup

and observe.

Level out

message?No Yes

**Look at reservoirsin back of unit.

(Open door on right

side, back)

Check the I-beam

tubing fromreservoir thru

pinch valve. (Fig. 2)

YesReplace I-

beam tubing**

No

Any bubbles

or leaks?

NoYes

Reconnect or

replace leakytubing

Problem

continues?

Yes

No Done


before performing

procedure.

Follow HELP.

Perform F16 to

Prime Diluent.

Did system

prime diluent?

Replace

Diluent pickup

tube.

No

Verify.

Run a

specimen.

Yes

Level out

message?

Yes

Press Startup

and observe.

No

ReticClear

ReticStain

PackPrsrv

PackLyse Cleaner

Fig. 2

CBCLyse

Fig. 2

Sensor Connector

Fig. 1

Is I-beam

tubing

crimped?


53/71


41 of 59

Backwash Tank Not Full MessageWhenever performing any troubleshooting procedures, always wear all required personal protective

equipment.


54/71


42 of 59

Ensure all materials are on hand including a 20 mL

syringe with a piece of yellow-striped tubing (5-inch)

attached to the tip and some distilled water.

Remove the left diluter panel by lifting up and out on

the panel.

Locate VL-107A, the white pinch valve located

above the Backwash Tank. The top fitting above VL-

107A is a feed-through fitting marked FF-5.

Disconnect the I-beam tubing from FF-5.Fill the syringe with distilled water and attach the

syringe tubing to FF-5.

Vacuum draws the water out of the syringe. If

needed, you may push on the plunger if the water

does not pull on its own.

The water fills the Vacuum Overflow Tank (VC 22)

Use enough water so that VC 22 is half full.

Disconnect the syringe tubing and reconnect the I-

beam tubing to FF-5.

Press the Reset button on the Analyzer. VC 22

drains.Perform Diluter Function F-30 to activate the

Backwash Tank Check to confirm resolution.

Run a blood sample to verify Instrument

performance.

Flush Backwash Tank Vacuum Supply Line

Return to

Backwash Tank

Not Full flowchart


55/71


43 of 59

Probe MessagesWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.


56/71


44 of 59

Rocker Bed MessagesWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.

Start

Done

Call BCI

Any physical

obstruction?

Message:Bed not Forward

Bed not BackwardBed not Level

F95 release

all solenoids

Manually rockthe bed to seeif there is any

physicalobstruction.

Remove it/Fix it

Yes

Clean

sensors withcanned air.

No

Locate the

sensors at the

right side of the

rocker bed (seeFig. 1 & 2)

Message

gone?

Yes

No

Start

Message:

Bed Not

Advanced

Place a sample

cassette with tubes

on the bed and

slide under tubedetector.

Any

obstruction?Call BCIYes

Perform the As-needed

Rocker Belt Procedure

Wipe belt with moistgauze (DI water) top andbottom. Pull belt from right

to left to clean all.Dry thoroughly, top and

bottom.

No

Use F96 toverify beltmovement.

MovementOK?

Done

Call BCI

Yes

No

Fig. 1 Top view

Fig. 2 Side view

Flag in

sensorBed Not

Backward

Bed Not

Level

Bed NotForward


57/71


45 of 59

Aspiration MessagesWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.

Start

Done

Call BCI

Aspiration

message and

alarm received

Check theHelp

information for the

particular aspiration

message (N, B, P, C)

received

Is the message

occurring on more

than one sample?

Check the sample tube

for correct blood

volume (at least 1mL of

properly collected

blood)

No

Clean and

exercise shear

valve 85/126

Yes

Perform the as needed Clean Shear

Valves Procedure

1. Locate shear valve 85/126.

2. While watching the ceramic in the left

side, activate Sol 126. Observe the space

created between the ceramic and the

metal.

3. Clean this space with DI water and dry

with lint-free tissues.

4. Press Stop three times to exit the

solenoid function.

5. Rerun sample(s).

Check the

sample tube

for clots.

Check if Hgb

result


58/71


46 of 59

Read all the instructions before beginning the procedure.

Needed:

COULTER cleaning agent

10-mL syringe (PN 2523145)

5 (12.7 cm) yellow-striped tubing (PN 3213176)

Cassette

Three (3) previously analyzed samples

1. Ensure that all safety precautions are followed while servicing BCI equipment. This

includes (but is not limited to) using proper lab protocol, identifying potential blood borne

pathogen exposure, using personal protective equipment, and properly disposing of parts.

2. Power off the instrument and power supply.

3. Push the aspiration tip for the manual mode all the way back in.

4. Fill syringe with 4 mL COULTER cleaning agent (more than 4 mL will result in spillage).

5. Connect 5 (12.7 cm) yellow-striped tubing to syringe.6. Remove tubing at fitting FF160 (refer to FIGURE 1). FF160 is located approximately five

inches (12.7 cm) to the left of the blood sampling valve and five inches (12.7 cm) from the

front of the diluter.

7. Connect yellow-striped tubing from syringe to fitting FF160.

8. Inject the COULTER cleaning agent into FF160. The fluid will fill needle bellows (rubber

sleeve surrounding the needle) pressure should be minimal.

9. Exercise the syringe (push plunger back and forth) to ensure that pathway is clean.

10. Remove yellow-striped tubing from FF160 and reattach brown-striped tubing from VL-9B

to fitting FF160.

11. Power up system.

12. Perform Start-up.

13. Run bloods to verify problem resolution.

Flush Aspiration Pathway Between the Needle and Aspirate Pump

Return to Aspiration

Messages Flowchart

Fig. 1


59/71


47 of 59

No Diff ResultsWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.


60/71


48 of 59

No Retic ResultsWhenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.


61/71


49 of 59

Dispense Probe MessagesOn LH SlideMaker

Use the diagrams for Dispense Probe Mechanism and Slide Transport Mechanismon pages 34 and 35 of this guide to help locate the sensors.

Whenever performing any troubleshooting procedures, always wear all required personal protectiveequipment.


62/71


50 of 59

Low Vacuum 1 or 2Messages on LH SlideMaker



63/71


51 of 59

Shuttle or Truck VacuumMessages on LH SlideMaker



64/71


52 of 59

Bath Not Draining MessagesOn LH SlideStainer



65/71


53 of 59

Bath Not Filling MessagesOn LH SlideStainer



66/71


54 of 59

As Needed Cleaning Procedures

LH 700 Series

Air FiltersAspiration Probe Wipe

BSV Drip Plate

BSV Outside

Clogged Flow Cell

Needle Assembly

Rocker Bed Belt

Stripper Plate

Shear Valves

LH SlideMaker

Air filter

Blood Spills and Broken Slides

Catch Tray

Dispense Probe and Rinse Cup

Priming/Backwashing

Flushing the SlideMaker

LH SlideStainer

Basket Holders

Basket Tray (s)

Bath Liquid Level Sensor

Bath Tray

Bath Tray Overflow Sensor

Drain and Fill Tubes

Dryer and Adjacent PlateHolding Position Tray

Plastic Baths

Tubing Tray

Flushing Stain Baths and Tubing


67/71


68/71


56 of 59

LH SlideMaker

As Needed Cleaning Procedures

To access the following cleaning procedures in HELP:

select Contents>LH SlideMaker>Cleaning Procedures

COMPONENT PROCEDURE TITLE

Air filter Cleaning and Replacing Air Filter (SlideMaker)

Blood Spills and Broken Slides Cleaning Blood Spills and Broken Slides

Catch Tray Cleaning a Catch Tray

Dispense Probe and Rinse Cup Cleaning the Dispense Probe and Rinse Cup

Priming/Backwashing Priming/Backwashing SlideMaker

Flushing the SlideMaker Flushing the SlideMaker (Bleach System)


69/71


57 of 59

LH SlideStainerAs Needed Cleaning Procedures

To access the following cleaning procedures in HELP:

select Contents>LH SlideStainer>Cleaning Procedures.

COMPONENT PROCEDURE TITLE

Basket Holders Basket Holders

Basket Tray Basket Tray

Bath Liquid Level Sensor Bath Liquid Level Sensor

Bath Tray Bath Tray

Bath Tray Overflow Sensor Bath Tray Overflow Sensor

Drain and Fill Tubes Drain and Fill Tubes

Dryer and Adjacent Plate Dryer and Adjacent Plate

Holding Position Tray Holding Position Tray

Plastic Baths Plastic Baths

Tubing Tray Tubing Tray

Flushing Stain Baths and Tubing Flushing Stain Baths and Tubing


70/71

Optimiz

ingStainProtoc

ols

OverallStainQuality

BeckmanCoulterhasdevelopedBCITruColorreagentsa

nddefaultprotocolsforuseonthe

LHSlideStainer.TheBeckmanCo

ulterdefault

protocolsaretoapredefinedcolorationspecification(Seeta

blesonnextpage).Sincecoloratio

nissubjective,optimizationofthe

protocolsand/or

reagentsmaybenecessa

rytomeetyourindividualcoloratio

ncriteria.Ensurethatboththemethanolandwateryouusemeetthe

specifications

detailedinyoursystems

onlineHelp.

Typicaltimeadjustm

entsincrements

Stain30seconds

Buffer1minute

Coloration(red/blu

e)isprimarilycontrolledbythepHofthebuffer.

Stainintensityanddifferentialstainingcharacteristicscanarecontrolled

by:

thelengthofstaining

times(longer=moreintense)

decreasingthebuffer

strength

concentrationofstain

inbuffer(morestain=darker,moreintense)

Symptom

Ca

use

Action

Bu

ffertooacidic

a)Adjustwithalkalinesolutionuntilyouachievethedesiredcolor.

b)

Trya

bufferwithahigherpH.

Excessivewashing

a)Shortenrinsetime(Bath5)untilyouachievethedesiredcolor.

b)AdjustfrequencyofBath5drainandfilluntilyouachievethedesiredcolor.

All

reagentstooold

Drainand

fillallbaths.

Staintimetooshort

Prolongstainingtimeuntilyouachievethedesiredcolor.

Toored

Bu

ffertimetoolong

Shortenb

uffertimeuntilyouachievethedes

iredcolor.

Bu

ffertooalkaline

a)Adjustwithacidsolutionuntilyouachieve

thedesiredcolor.

b)TryabufferwithalowerpH.

Ins

ufficientwashing

Lengthen

rinsetimeuntilyouachievethedesiredcolor.

Staintimetoolong

Decrease

staintimeuntilyouachievethede

siredcolor.

Tooblue

Bu

ffertimetooshort

Lengthen

buffertimeuntilyouachievethedesiredcolor.

Staintimetoolong

Shortenstaintimeuntilyouachievethedesiredcolor.

Toodark

Bu

ffertimetoolong

Shortenb

uffertimeuntilyouachievethedes

iredcolor.

Rinsetimetoolong

Decrease

rinsetimeuntilyouachievethede

siredcolor.

Po

orqualityrinsewater

a)Changewater.

b)Verifyw

atermeetsspecificationsofType

IINCCLSwater

Staintimetooshort

Increasestaintimeuntilyouachievethedes

iredcolor

Toolight

Bu

ffertimetooshort

Increasebuffertimeuntilyouachievethede

siredcolor


71/71

Bu

ffertooconcentrated

Dilutebuffersolutionwithdeionizedordistilledwateruntilyouachievethedesiredcolor

Ca

use

Action

Waterhascontaminated

the

methanol

a)Drain/F

illthemethanolbath.

b)Dipem

ptybasketinmethanolpriortore-e

ntryintosystem.

Staintooold

Drain/fillS

tainandStain/Bufferbaths.

Excessstainleftonslide

Addquick

rinsesteptoprotocol(primarilyforWGandMGG).SeehelptopicQuickRinse

Method.

StainorStain/buffertoo

od

Drain/Fillallbaths.

Rnse(Bath5)dirty

a)Drain/F

illBath5.

b)Increas

efrequencyofBath5drain/fill.

c)AddQu

ickRinsesteptoprotocol(primarilyforWGorMGG).SeehelptopicQuickRinse

Method

.

Stainbathsdirty(Stain

andStain/Buffer)

PerformtheBathFlushingProcedure.

Bo

odsmearmorethan

2h

oursoldpriortofixing

Makenew

bloodsmear.

res

entthestaincharacteristicsaga

instwhichtheBeckmanCoulter

andBCIdefaultprotocolswereeva

luated.

Refer

ence

ogy,

9th

edition,

Vol.1,page23.

olume12,

Number1.

ReferenceLeukocyteDifferentialCount(Proportional)andEvaluatio

nof

Man

ualofPeripheralCellMorphology,OConno

r,BarbaraH.,

1984,page1618.Copiedfrom:PN

4276448D

(November2003)

ColoringofRomanowskyStains

Structure

Cell

Color

Nuclei

All

Purple

Erythrocyte

Pink

Lymphocyte

Blue

Monocyte

Grayblue

Neutrophil

Pink

Metamyelocyte

Pink

Myelocyte

Pink

Cytoplasm

Promyelocyte

Blue

Granules

Basophil

Purple,

black

Eosinophil

Orange

Neutrophil

Purple

Platelet

Purple

HumanBloodCellInclusions

Description

Ce

ll

Color

S

picule-likeformation

Myeloblas

t

Pink

S

mallspots

Redblood

cells

Purple

Inclusions

Redblood

cells

Purple/blue

M

ediumsizedspot

Redblood

cells

Purple

T

hreadlikefilaments

Redblood

cells

Purple

S

mallgranules

Redblood

cells

Purple

C

oarseGranules

WBC

DarkPurple

S

pots

WBC

Sky-Blue

coulter lh 700 series

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