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BIOGRAPHICAL SKETCH

NAME Hernani Cualing, MD POSITION TITLEAssociate Professor Of Oncology and HematologyMedical Director, IHCFLOW, Inc.

EDUCATION/TRAINING

INSTITUTION AND LOCATION DEGREE(if applicable) YEAR(s) FIELD OF STUDY

University of the Philippines B.S. 1974 Premedical SciencesUniversity of the Philippines M.D. 1978 M.D.A. Positions

1979 Medicine Internship, University of the Philippines1980 Residency, Surgery1985.87 Research Staff fellow, Dana-Farber Cancer Inst., Boston,MA1987.90 AP/CP Pathology Residency,NCMC, SUNY Stony Brook, ,East Meadow, New York,1990-91 Surgical Pathology fellowship, Nassau County Medical Center , SUNY Stony Brook;

1991.92 Hematopathology Fellow, Indiana University Medical Center, Riley Childrens Hospital, Indianapolis,Indiana1994-02 Medical Student Elective Director Diagnostic Hematopathology, Year IV UC-1501461992-01 Assistant Professor of Pathology, University of Cincinnati Medical Center, Cincinnati, Ohio2000-01 Associate Director, Hematopathology Fellowship Program, University of Cincinnati, 2001-02

Associate Professor, University of Cincinnati Medical Center, Cincinnati, Ohio

2002- Associate Professor of Oncology and Hematology, University of South Florida, Moffitt Cancer Center andResearcInstitute2002-20010 Medical Director of Immunohistochemistry/Histology, Program of Pathology, Department of

Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida2006- 2010 Director, Hematopathology fellowship Training Program, University of South Florida, Moffitt Cancer Center andRes Institute2010 - Univ of South Florida College of Medicine Dept of Pathology and Cell Biology, Consultant Medical Director, IHCFLOW,Inc.

B. HONORS AND AWARDS

1969-1971 Scholastic Delegate to Physics, Chemistry, Biology Interscholastic Competi tionsAcademic Excellence-Top Academic Rank, Section Class # 1 of 26 classesSenior High School, Ramon Magsaysay High School,Manila, Philippines

1976-1977 President of Medical Student Society

University of the Philippines College of MedicineManila, Philippines

1989-1990 First Prize Award, Resident Presentation in Pathology,Zell Ballen Patterns in Organ of ZukerkandlNassau Society of PathologistEast Meadow, NY

1990-1991 Chief Resident, Department of PathologyNassau County Medical Center, SUNY at Stony Brook Teaching HospitalEast Meadow, NY

1992-1993 Finalist, American Society of Clinical PathologistSpring Resident Presentation CompetitionBoston, MA

1994-1995 Biomedical Engineering Seed Grant Award-Top Score

I Image Analysis of LymphomasUniversity of Cincinnati University- Seed Grant CompetitionCincinnati, Ohio

1997.2000 Biomedical Engineering Grant Award

High Order Decision Theoretic Diagnosis of LymphomasWhitaker Foundation ( National )

2000-2001 Stratmores Whos Who of North America

2003-2010 Marquis Whos Who, 58th, 60th, 61st, in North America


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H.Cualing Editor in Chief, Non-Neoplastic Hematopathology and Infections, Wiley: NJ, USA, 2011 in preparation.Alekshun TJ, Rezania D, Ayala E, Cualing H, Sokol L.Skeletal muscle peripheral T-cell lymphoma. J Clin Oncol.2008 Jan 20;26(3):501-3. IF: 13.598 PMID: 18202425J Pidala, Cualing H, Pinilla J. Acute basophilic leukemia arising from chronic myelogenous leukemia withdevelopment of t(7;8)(q34, q13) Cancer Genet Cytogenet. 2008 Apr 1;182 (1):46-9.Raychaudhuri J, Cualing H, Haider K, Kharfan-Dabaja MA.Granulocytic sarcoma and chronic lymphocytic leukemiaof the gastrointestinal tract after allogeneic hematopoietic cell transplantation mimicking graft-versus-host disease.

Leuk Lymphoma. 2008 Feb;49(2):350-2. IF: 1.559 PMID: 18231925Calder K, Khalil F, Schlauder S, Cualing H, Morgan M. Immunohistochemical expression of survivin in cutaneoussebaceous lesions. Am J Dermatopathol. 2008 Dec;30(6):545-548. Pubmedid: 19033926.Cualing H. Pathology of the Spleen. In Handbook of Hematologic Pathology, Schumacher H, Stass S. Eds. 53-73,Marcel Dekker, Inc., New York, April 2000.: Book ChapterMojdeh Naghashpour, Hernani Cualing* Splenomegaly with sea-blue histiocytosis, dyslipidemia, and nephropathy ina patient with lecithin-cholesterol acyltransferase deficiency: a clinicopathologic correlation, in press 2009Metabolism Clinical and ExperimentalMojdeh Naghashpour, MD, PhD; Hernani D Cualing, MD; Margaret Szabunio, MD;Marilyn M Bui, MD, PhD Hyaline-Vascular Castleman Disease: A Rare Cause of Solitary Subcutaneous Soft Tissue,in press, 2009 The American Journal of DermatopathologyManuscript Number: AJD-D-09-00143 *Condarco T, Sagatys E, Prakash A, Rezania D, Cualing H. Primary cutaneous B-cell lymphoma in a child. FetalPediatr Pathol. 2008 Dec;27(4):206-214. Pubmedid: 18800263.Cualing H,Moscinski LC, Zhong E. Virtual Flow Cytometry of Immunostained Lymphocytes on Microscopic TissueSlides, Clinical Cytometry part B, vol 72B, p 63-76, Jan 15, 2007.Cualing H, Khalil F, Heekin J, Lili Miles.The rate and morphometric pattern of bone marrow recovery post-

myelosuppressive therapy for acute leukemia: a quantitative study-in press Archives of Pathology and Lab Medicine2007Yoshinori Takahashi, Domenico Coppola, Norimasa Matsushita, Hernani D. Cualing, Yuya Sato, James J. Mul,Hong-Gang Wang.Regulation of autophagy-dependent cell death and tumorigenesis by Bif-1. in press Nature CellBiology 2007.Droc C*, Cualing H., Kadin M. The Need for an Improved Classification For Therapy of CD30 + lymphomas. CancerControl Journal, vol 14, no. 2, pp 124-132, April 2007.Rezania D*, Cualing H. Aggressive Peripheral T cell Neoplasms and subtypes with high predilection in the Skin.Cancer Control Journal, vol 14, no. 2, pp 112-123, April 2007Rezania D*, Cualing H, Ayala E. Diagnosis, Management, And The Role Of Hematopoietic Stem CellTransplantation in Aggressive Peripheral T Cell Neoplasms Cancer Control Journal, vol 14, no. 2, pp 151-159, April2007Connie Keehn*, Cualing H., Marginal Zone Lymphoma with a Dual Cutaneous and Leukemic Presentation and anAberrant MyeloidMonocytic Phenotype. Journal of Cutaneous PathologyVolume 33: 6, 236-241, March 2006.Breneman DL, Raju US, Breneman JC, Steele PE, McFadden DW, Cualing HD, Nussbaum MS, Swerdlow SH.Replyto Letters to the Editor: Lymph node grading for staging of mycosis fungoides may benefit from examination ofmultiple excised lymph nodes. J Am Acad Dermatol. 2004 vol51:Sept 2004, 483-484.Colvin JH, Lamerson CL, Cualing H, Mutasim DF. Cutaneous lymphoplasmacytoid lymphoma (immunocytoma) withWaldenstrom's macroglobulinemia mimicking rosacea.J Am Acad Dermatol. 2003 Dec;49(6):1159-62.Breneman DL, Raju US, Breneman JC, Steele PE, McFadden DW, Cualing HD, Nussbaum MS, SwerdlowSH.Lymph node grading for staging of mycosis fungoides may benefit from examination of multiple excised lymphnodes. J Am Acad Dermatol. 2003 May;48(5):702-6.Goldman A*, Bedolla G, Gebrail F, Cualing H, Bronchus-Associated Lymphoid Tissue Lymphoma (BALTlymphoma). Vol 127, 115-116, Jan 2003, Archives of Pathol and Lab Med. (Direct supervision of medical studentelective in Diagnostic Hematopathology)Kulow G, Breneman D, Cualing H, Steele P, Breneman J, Van Horn J, Mutasim D. The Progression of Cutaneous

B-cell Pseudolymphoma (CBPL) to Cutaneous B-cell lymphoma (CBCL). Vol 6(6), 519-528, Nov 2002, J ofCutaneous Med and Surg.Cualing H, Schroder L Perme I. Allergic Granulomatosis secondary to a Limited Churg-Strauss Lymphadenopathy:Case Report with Histologic and Immunophenotypic analysis- 125:954-957, 2001 Arch of Pathol Lab Med July 2001Cualing H. Perspectives: Automated Analysis in Flow Cytometry.Communications in Clin Cytometry, 42:110-113,

April 2000.*Cualing H, Zellner D, Steele P. Blastic transformation of splenic marginal zone B-cell lymphoma.. Arch Pathol LabMedicine. 124(3):748-752 March 2000*Marcelo A, Fontelo P, Farolan M, Cualing H. Effect of Image Compression on Telepathology: A RandomizedControlled Trial. Arch Pathol Lab Med, 124:1653-1656, Nov 2000.Cualing H, Balachander T, Kothari R. Immunophenotypic diagnosis of acute leukemia using decision tree analysis.Laboratory Investigation , 79(2): 205-212, Feb 1999.*Craig A*, Cualing H, Thomas G, Lamerson C, Smith R. Cytophagic Histiocytic Panniculitis- a syndromeassociated with benign and malignant panniculitis: Case comparison and review of the literature. J Amer AcadDermatol 39(5):721-736, part 1 Nov 1998.*Massela F, Kowal-Vern A, Shrit A, Wibowo A, Rector J, Cotelingham J, Collier J, Michael A, Cualing H,Schumacher H. Acute Erythroleukemia. Am J Clin Pathol 110(5):590-598, Nov 1998.


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Cualing H, Vasireddy R. Adult T-cell leukemia and Cutaneous pathology: An overview. J Geriat Dermatol 5(8): 377-382, 1997.*Balachander T, Kothari R, Cualing H. An empirical comparison of dimensionality reduction techniques for PatternClassification. Gerstner W et al eds. Artificial Neural Networks ICANN 97, Lausanne, Switzerland. Lecture Notes inComputer Science1327: 589-594, 1997.Cualing H, Povoski S, Fenoglio-Preiser C, Yassin R, Bell R. Characterization of Hematologic malignancies in SyrianGolden Hamsters, Carcinogenesis 17(9):1983-1988,1996.*Kothari R, Cualing H. , Balachander T. Neural Network Analysis of Flow Cytometric Immunophenotype

Data. IEEE Transactions in Biomedical Engineering, 43(8):803-810,Aug 1996.Orazi A, Neiman R, Cualing H, Heerema N, John K. , CD34 Immunostaining of Bone marrow biopsy specimens is areliable way to classify the phases of chronic myeloid leukemia. Am J Clin Pathol, 101(4):426-428, Apr 1994.Book Chapters:Cualing H. Pathology of the Spleen. In Handbook of Hematologic Pathology, Schumacher H, Stass S. Eds. 53-73,Marcel Dekker, Inc., New York, April 2000.Book: Cualing Hernani, Editor: NonNeoplastic Hematopathology and Infections, Wiley Blackwell, NY, manuscript inpreparationC. Past Research Support

Account #: MCC 13840 (Hernani Cualing MD)Dates: 2005-2008Source: Lymphoma Foundation of AmericaTitle: A Phase II Trial Using a Universal GM-CSF-Producing and CD40L-Expressing Bystander Cell Line(GM.CD40L) in the Formulation of Autologous Tumor Cell-Based Vaccines for Patients with Mantle Cell Lymphoma.% Effort:2 % Goal: Translational investigation of the clinical and histopathologic factors associated with responders:Role in the Study: Hematopathologist-Tissue Quantification of tumor infiltrating lymphocytes in mantle cell

lymphoma.Clinical Trial 2006-Medarex ALCL phase I trial using Anti-CD30, Moffitt Cancer Center, Coinvestigator, 2 % effort.2005-2007 Translational study on immunomodulating monoclonal antibody treatment for peripheral T celllymphomas. Role: Hematopathologist and quantification of target antigenClinical Trial 2006-Medimmune anti CD2, Coinvestigator, 2 % effort. Moffitt Cancer Center,2006-2007Translationalstudy on immunomodulating monoclonal antibody treatment for peripheral T cell lymphomas. Role:Hematopathologist quantifying target antigen.

2007 Division of Hematopathology Internal Research Funding for T regulatory tumor infiltrating lymphocytes inlymphomas. Role: Principal investigator 5 % effort.PENDINGApplication PI/George Blanck, Ph.D. Co Investigator/Hernani Cualing, MDSource: National Institute of HealthTitle:Mouse modeling of a CD30+ lymphoproliferative disorder: Role of IRF-1 defects in ALCLs% Effort 10%Role in Study: Co PI: Hematopathologist comparing human CD30+ lymphomas with mouse CD30+ lymphomasNew Multiinstitutional Grant 9: Exploratory/Developmental Research Grant (R21) via American Recovery &Reinvestment Act of 2009 as RFA to Translational Science and specific Challenge Topic 15-CA-105, The Biology ofCancer in Adolescents and Young Adults.: using tissue blocks from SEER tissue banks from Hawaii, Iowa, RI, USCSamples consist of diagnostic tumors from 527 Hodgkin lymphoma patients in the National Cancer InstituteSurveillance, Epidemiology and End-Results (SEER) program Residual Tissue Repository (RTR). In Los Angeles,Hawaii and Iowa.% effort: 3 %Role: To provide hematopathology support and know-how for automated virtual flow analysis of T cell subsetsimmunostains on these blocks.

PATENTS and TRADEMARKS1. USPTO/Virtual flow cytometry on immunostained tissue-Tissue cytometer -awarded2. USPTO/ International PCT :Automated Cytomics: Bone Marrow Cellularity Determination-pending3. USPTO/ Automated Cytomics: Bone Marrow Reticulin Fibrosis Determination4. USPTO Registered Trademark: VirtualFlow IHCFLOW

D. Teaching and LecturesApril 28, 2007 Co-director of 1st Annual Cutaneous Lymphoma Seminar, Moffitt Cancer Center, Tampa,

Sponsored by Moffitt Cancer Center Foundation and jointly with Cutaneous LymphomaFoundation- Update on Skin Lymphomas

April 19, 20 2008 Director of 2nd Annual Cutaneous Lymphoma Seminar,Sponsored by Cutaneous Lymphoma Foundation, USF Health, Moffitt Cancer Institute

and Funded by Eisai Corporation, and other National Pharmaceutical CorporationsLake Buena Vista Resort, Orlando FloridaThis two day seminar included three patients as speakers and Faculty from International CutaneousLymphoma Study Group, Harvard Medical School Faculty and attended nationally by Dermatologist,Pathologists, Oncologists and some attendees from out of the country.

Teaching Series

1992-2010 Hematopathology Lecture Series for Residents and Fellows


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Hematopathology Fellow Teaching -Slide Weekly Slide Workshop SeriesHematopathology Fellow Teaching -Slide Weekly Slide Workshop Series

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