CultivationCultivation

To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.

Cultivation

Bacteria

Plant

AnimalInsect

Methods of animal virus Methods of animal virus cultivationcultivation

1.Organized tissue.2.Cell culture.3.Laboratory animals.

Chick embryo technique Chick embryo technique

Methods of animal virus Methods of animal virus cultivationcultivation

1.Organized tissue.2.Cell culture.3.Laboratory animals.

Cell cultureCell culture

1. Removal.2. Homogenization.3. Washing.4. Trypsinization.5. Washing.6. Growing.7. Inoculation.8. Incubation.

Methods of animal virus Methods of animal virus cultivationcultivation

1.Organized tissue.2.Cell culture.3.Laboratory animals.

IntracerebrallyIntracerebrally

IntraperitoneallyIntraperitoneally

IntramuscularlyIntramuscularly

IntravenouslyIntravenously

IntranasallyIntranasally

Types of cell culture

Primary

Diploid

Suspension Permanent

Primary cell culturePrimary cell culture

A culture derived directly from a tissueA culture derived directly from a tissue

Diploid cell cultureDiploid cell culture

Subsequent cultures derived from primary cell culture.Subsequent cultures derived from primary cell culture.

Suspension cell cultureSuspension cell culture

A culture in it the dispersed cells are not allowed to settle down, but A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.they are kept floating by continuous shaking of the flasks.

Permanent cell linePermanent cell line

A culture derived from single separated cell i.e. diploid culture it can be A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely. propagated indefinitely.

Cultivation of plant Cultivation of plant virusesviruses

• Protoplast and callus method.• Mechanical method.• Biological method.

Cultivation of plant Cultivation of plant virusesviruses

• Protoplast and callus method.• Mechanical method.• Biological method.

InoculationInoculation

IncubationIncubation

InoculationInoculation

IncubationIncubation

Cultivation of plant Cultivation of plant virusesviruses

• Protoplast and callus method.• Mechanical method.• Biological method.

Cultivation of plant Cultivation of plant virusesviruses

• Protoplast and callus method.• Mechanical method.• Biological method.

IncubationIncubation

Cultivation of bacterial Cultivation of bacterial virusesviruses

1.1. On broth mediaOn broth media 2. On solid medium2. On solid medium

Bacteria + solid agarBacteria + solid agar

Virus + semisolid agarVirus + semisolid agar

Cultivation of Insect virusesCultivation of Insect viruses

Criteria of purityCriteria of purity

Chemical purityChemical purity

Physical purityPhysical purity

Biological purityBiological purity