Abstracts of the Articles Printed in Hakkokogaku Kaishi 577

Abstracts of the Articles Printed in Hakkokogaku Kaishi

Vol. 64, No. 6 (1986)

Cultural Condition for Cell Mass Production of Aureobasidium pullulans from Solvolysis pulp- ing Waste Liquor. JUN HOSOKAWA, KAZUTOSHI

Y0SHIHARA, M.A~HI NXSHPgA~tn, and HIROSHI K A m S H ~ (Government Industrial Research Institute Sikoku, 2--3-3 Hananomiya-cho, Takamatsu-shi 761, Japan) Hakkokogaku 64: 473-477. 1986. The optimum cultural conditions were investigated

for the cell mass production of strain A. pullulans IFO 6353 which has found to be the most promising organism that uses xylan in the solvolysis pulping waste liquor.

The optimum cultural conditions were 29°C, initial pH 6.0-6.5. Ammonium sulfate and L- asparagine were suitable as supplementary nitrogen sources. The optimum concentrations of supplemental nutrients were 0.5% ammonium sulfate, 0.5-1.0% potassium dihydrogenphosphate, and 0. 1% magnesium sulfate. Further, the addition of yeast extract greatly increased the cell mass production.

The cell mass reached 7.5 g per l of medium after 3 days under the optimum conditions (yeast extract, 0.25%). After 3 days of cultivation, A. pullulans IFO 6353 had used about 70% of the mono-, oligo-, and poly-saccharides in the waste liquor as one of the carbon sources.

Production of Fungal Cell Wall Lytlc Enzymes by Oerskovia sp. CK and Some Propertics o f the Crude Enzymes . KATSVYA GOMX, DAUmO

HOSAKA, NAOTO OKAZAKI*, TOSHIO TANAKA*, CHIEKO KU/~AGAI, YUZURU IIMURA, and SHODO HARA (National Research Institute of Brewing, 2-6-30, Takinogawa, Kita-ku, Tokyo 114; *Office of Technical Officers, Kanto Shin-etsu Regional Taxation Bureau, 1-3-2, Ohtemachi, Chooda-ku, Tokyo 100, Japan) Hakkokogaku 64: 479-485. 1986. Oerskovia sp. CK was cultivated with the mycelia

of koji mold (Aspergillus oryzae) and produced fungal cell wall lytic enzymes inducibly. When chitin and dry yeast were used as substrates instead of fungal mycetia, the productivity of the lyric enzyme increased greatly. When the culture conditions for enzyme production were investigated, the maximum lytic activity was found at 30°C for 40 h in a medium containing 1% chitin powder and 0.5% dry yeast (pH 8.0). I t was proved that chitlnasc was the main

contributor to the lytic activity on ftmgal cell walls. Optimum temperature and pH of the lytic activity

were 45°C and 6.0-7.0, respectively. The lytie activity was stable at pH 5.0-8.0, but completely inactivated at 60°C for 15 rain.

The enzyme had high lytic activity toward the cell walls of the various fungi tested except for the Zygomycetes group. This enzyme could be useful for protoplasting of filamentous fungi.

Ethanol Fermentatlon System Combined with Membrane Separator o f Pervaporation. KANJI

]V[_ATSUMOTO, TOSHIHIRO SUGIYAMA, and HARtrmKO Om~'A (Department of Material Science and Chemical Engineering, Faculty of Engineering, Yokohama National University, Tokiwadai, Hodogaya-ku, Yokohama 240, Japan) Hakkokogaku 64: 487492. 1986. The characteristics of continuous ethanol fer-

mentation system with membrane separator of pervaporatlon were investigated. The membrane used is of hydrophobic microporous hollow fibers of polypropylene. An ethanol-water mixture of high ethanol concentration was recovered continuously and directly from the fermentation broth. Intermittent backflnshing by compressed nitrogen gas prevented degradation of the permeation rate. The selectivity of the membrane for ethanol was a little bit smaller than that of distillation at normal pressure. The permeation rate of ethanol was almost llnearly pro- portional to the ethanol concentration of feed broth. The carbon dioxide gas generated by fermentation promoted the permeation rate. The ethanol con- centration of permeation was estimated by the fermentation dynamic equations.

Stimulation of f i lamentous Bulking of Activated Sludge by Lactic Acid, Acetic Acid, and Glucose.

RIKIYA TAKAHASHX, AKIHIKO SUZUKI, MASAHARU Suz~x, and HITOSHI YONEYAMA (Department of Brewing and Fermentation, Tokyo University of Agri- culture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156, Japan) Hakkokogaku 64: 493--498. 1986. An artificial sewage which contained small

amounts of organic acids, such as acetic acid or lactic acid stimulated filamentous bulking of normal sewage activated sludge as well as glucose-containing artificial