Tissue Culture

Tissue CultureBiotechnologyFeb 2012The GuideFunFruitNutritionBy:Afida Zahara AdzkiyaAlifah Farah GusmaniarIndah Puspita AzizRidha Astri IndryanaBalanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Definition of tissue culture

FunFruitNutritionTissue culture is an activity to cultivate a tissue of plant vegetatively to be a plant that has similar property to its parent in a short time. Plants can be multiplied using this technique as plants have a totipotency ability, meaning that from a tissue they can grow and develop into new individuals. Tissue culture is used to solve food shortage problems.

Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Tissue culture was found and well known by F.C Steward, a plant physiologist from United States. Who did his research in 1989. Steward try to make tissue culture of carrot and he is success. Tissue culture well because the work of Muer, Hildebrant, and Riker in 1954.

Information Layout Slide with TableFunFruitNutrition

Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.The Various Uses of Tissue CultureObtaining a lot of germs in a short time with similar property to the parent.Making plants with properties we want.Tissue culture is economical from aspect of time , space, and energy.Produce plants free from virus and disease.FunFruitNutrition

Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.The Procedure of Tissue Culture TechniqueBalanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.1Preparing growth medium.Substance that must exist in growth medium consist of minerals (macro element, such as C, H, O, N and micro element such as Zn, Mo), amino acid, vitamin, sugar, and hormone in certain amount.

Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.2Preparing tissue.The tip part of a young shoot, such as root and leaf tips, are cut into 1-1.5 mm. Next, the tissue is sterilized using 5% hypochlorite calcium and is washed off several times with sterile aquadest.

Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Cultivating tissue (explants) in liquid growth medium.Explants are placed in a mixer table (shaker) that shakes at 60-70 times per minute. The mixing is performed for 6 hours a day for 1.5 2 months . Shaking is meant for faster distribution of substance and food absorption as well as process of gas exchange. In about 2 months, callus will grow in this liquid medium.

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Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Moving plant whose root bud and leaf bud have grown to another solid growth medium.

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Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Cultivating plant in pot containing soil.The plants whose rot bud and leaf bud have grown are removed to another solid medium to be separated, so bigger plants can grow.

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Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.6

Cultivating plant in actual land.Before being cultivated in actual land, the plant from solid medium is moved or cultivated first in a pot that contains soil that has been given fertilizer. If the condition of plant has been strong, it can be moved to actual land.

Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Question Time

Apakah objek yang dikultur oleh pempopuler tekhnik kultur jaringan (Muer, Hildebrant, and Riker) sama dengan objek yang dikultur oleh F. C. Steward?Tidak sama.Hildebrant berhasil mengembangkan tekhnik kultur jaringan pada sel-sel rambut dari jaringan mesofil daun tanaman monokotil.Muer berhasil menumbuhkan tanaman lengkap dari kultur sel tunggal.Kalau Riker kami belum mengetahui apa objek yang dikulturnya, namun pada logikanya itu pasti berbeda dengan objek yang dikultur oleh F. C. Steward.Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.Question TimeQuestion from Sabila:What kind of plant that usually used for tissue culture?

Pada dasarnya semua jenid tumbuhan dapat dikultur, karena semua tumbuhan memiliki sifat totipotensi di jaringan meristem (ujung akar dan batang). Namun tumbuhan yang biasanya dikultur adalah:Yang pertumbuhannya lambat atau susahMemiliki nilai jual yang tinggiBanyak bergunan bagi masyarakatContohnya adalah tanaman anggrek, jati dan tebu.Balanced Nutrition Coming to Fruitionwww.funFruit.com1987 Street Ave City, State60623, US.