custom assay development services · 2019. 6. 14. · hdac6 activity assay kit (f) k466 cl, pp, tl...

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Custom Assay Development Services

Ordering: Email: [email protected] | Tel:408.493.1800 | Web: www.BioVision.com

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ORDE R METHOD: Phone: 1-800-891-9699 Email: [email protected] Internet: www.BioVision.com Mail to: BioVision, Inc., 155 South Milpitas Blvd., Milpitas, CA 95035 USA

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TERMS:Net 30 days in U.S. dollars after credit approval.

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WARRANTY:BioVision’s products are warranted to meet our product specifications in effect at the time of shipment. Notice of nonconforming products must be made to BioVision within 30 days of receipt of the product. This product warranty limits BioVision’s liability to the replacement of the product only. In no event, shall BioVision be liable for any kind of loss or damage resulting from the use of BioVision’s products.

Note: If the above terms are not acceptable to you, promptly return any products in the package in which they were received. Failure to promptly return the product or any use of the product constitutes acceptance of the above terms.

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RESEARCH USE ONLY: All products described in this catalog and on our web site are for research purposes only, not to be used for diagnostic or therapeutic purposes. They must not be used with human subjects.

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Order Instructions & Warranty Information

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Tech Help: Email: [email protected] | Fax: 408.493.1801 |

Having the right people working in your laboratory with the right equipment at the right time can be a challenge. As scientists, our goals are not to run assays but rather to get results. Scientists at BioVision have decades of experience developing and running cell-based and biochemical assays.

At the request of many of our customers, we are pleased to offer custom assay services. Simply send us your samples and we will deliver the results.

Choose from over nine hundred assay kits. Protocols for all of these assays are conveniently available on our website. There is no need to spend your valuable time developing SOPs for these assays. If you choose to bring the work back in-house, our assay kits are commercially available to maintain consistency and reproducibility within your projects.

This brochure lists some of our most popular assays, but there are many more available. If you are looking for an assay or do not know what assay(s) are best for your work, our team of experienced Ph.D. scientists can assist you with that too.

• Non Radioactive, homogeneous assays• Specific and convenient: Minimal sample preparation, Fast protocols (1-2 hours)• Cost effective: 100 assays, High throughput screening compatible• Validated: Using mammalian tissues, cells, and biological fluids• Excellent personalized support throughout in project

Key Features of Our Assays:

Project Inquiry & Initial Discussion

Optimization with Our Specialists

Custom Proposal for Your Project

Your Project Starts

Online Updates

High Quality Products

Inquiry CustomRequirements

QuoteProject

InitiationUpdate Deliverables

Contact us by email or by telephone to discuss your assay requirements. We will get back to you within 48 hrs with reason-able quotes. Your project will start after contract is in place with an agreement. You will get regular project updates. You will be happy to receive high quality and reliable service.

Don't wait, Call us & save your time. Here is how the process works.

Contact us at: [email protected] Number: 408.493.1800

Custom Assay Services

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Biochemical Assays

Drug Metabolism AssaysThe disposition of a drug or xenobiotic (any foreign chemical) within a living organism encompasses all reactions/physiological mechanisms resulting in Absorption, Distribution, Metabolism and Excretion (ADME) of the molecule. Here we present a collection of kits to study various aspects of ADME.

Product Name Cat. No. Sample TypeAromatase (CYP19A) Activity Assay Kit (F) K983 CL, O, TL

Aromatase (CYP19A) Inhibitor Screening Kit (F) K984 IC

Cytochrome P450 1A2 (CYP1A2) Activity Assay Kit (F) K893 CL, O, PP, TL

Cytochrome P450 1A2 (CYP1A2) Inhibitor Screening Kit (F) K894 NA

Cytochrome P450 2C19 (CYP2C19) Activity Assay Kit (F) K848 CL, O, PP, TL

Cytochrome P450 2C19 (CYP2C19) Inhibitor Screening Kit (F) K849 NA

Figure 1: Adapted from https://www.intechopen.com/books/risk-management-trends/system-building-for-safe-medication

(C)=Colorimetric | (CB)=Cell Based | (F)=Fluorometric | (FC)=Flow CytometryAB=Antibody | CB-Intact Cells | CL=Cell Lysate | IC=Inhibitor Compound | O=Other | P=Plasma | PP=Protein Preparation | S=Serum | TL=Tissue Lysate | U=Urine

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The development and maintenance of a multicellular organism is orchestrated by a set of chemical reactions that switch parts of the genome off and on at strategic times and locations. The non-genetic cellular memory, which records developmental and environmental cues, is the basis of epi-(above)–genetics. BioVision has an extensive portfolio of assay kits geared towards unraveling the mysteries of the epigenetic system.

Click here for a complete list of Drug Metabolism Assays.

Epigenetic Assays

Product Name Cat. No. Sample TypeCytochrome P450 2C9 (CYP2C9) Activity Assay Kit (F) K895 CL, O, PP, TL

Cytochrome P450 2C9 (CYP2C9) Inhibitor Screening Kit (F) K896 NA

Cytochrome P450 2D6 (CYP2D6) Activity Assay Kit (F) K703 CL, O, PP, TL

Cytochrome P450 2D6 (CYP2D6) Inhibitor Screening Kit (F) K704 NA

Cytochrome P450 Activity Assay Kits (F) - 3A4, 2D6, 2C19, 1A2, 2C9 K701 CL, PP, TL

Cytochrome P450 Inhibitor Screening Kits (F) - 3A4, 2D6, 2C19, 1A2, 2C9 K702 NA

Cytochrome P450 Reductase (CPR) Activity Kit (C) K700 CL, O, PP, TL

Multidrug Efflux Transporter (MDR1/P-gp) Ligand Screening Kit K507 CL

UGT Activity Assay / Ligand Screening Kit (F) K692 CL, PP, TL


(A) (B) (C)

Figure 2: Aromatase (CYP19A) Activity Assay Kit (Fluorometric) (Cat. No. K983) (A) Standard curve of Aromatase Substrate metabolite fluorescence. One mole of fluorescence standard corresponds to the metabolism of one mole of Aromatase Substrate. (B) Reaction kinetics of fluorogenic substrate metabolism in donor-pooled human placental microsomes (0.5 mg/mL) at 37°C in the presence and absence of the competitive aromatase inhibitor letrozole (the no inhibitor condition contained assay buffer with 0.2% acetonitrile as a solvent control). (C) Specific activity of aromatase in human placental microsome samples (mean ± SEM of four independent replicates). Assays were performed according to the kit protocol.

https://www.biovision.com/products/drug-metabolism-and-disposition.html/

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(A) (B) (C)

Click here for a complete list of Epigenetic Assays.

Figure 3: HAT Activity Fluorometric Assay Kit (Cat. No. K334): (A) Co A Standard Curve. (B) HAT Activity in HeLa Nuclear Extract. (C) Specific Activity of HeLa Nuclear Extract and purified recombinant pCAF. Assay were performed following the kit protocol.

Product Name Cat. No. Sample TypeHAT (H4) Activity Assay Kit (F) K336 CL, PP, TL

HAT Activity Assay Kit (C) K332 CL

HAT Activity Assay Kit (F) K334 CL, PP, TL

HDAC Activity Assay Kit (C) K331 CL, PP, TL

InSitu HDAC Activity Assay Kit (F) K339 CL

HDAC Activity Assay Kit (F) K330 CL, PP, TL

HDAC Inhibitor Drug Screening Kit (F) K340 IC

HDAC-5 Inhibitor Screening Kit (F) K171 CL, TL

HDAC6 Activity Assay Kit (F) K466 CL, PP, TL

HDAC6 Inhibitor Screening Kit (F) K465 IC

HDAC8 Activity Assay Kit (F) K348 CL, PP, TL

N'-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay K822 NA

p300 Inhibitor Screening Kit (F) K346 NA

pCAF Inhibitor Screening Kit (F) K345 IC

SIRT1 Inhibitor/Activator Screening Kit (F) K325 NA

SIRT2 Inhibitor Screening Assay Kit (F) K322 NA

SIRT6 Inhibitor Screening Kit (F) K323 NA

Sirtuin Activity Assay Kit (F) K324 CL, O, PP, TL

https://www.biovision.com/products/epigenetics/other-epigenetics-products/assay-kits.html

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Popular Kits Cat. No. Sample TypeAmino Acid Assays (No. of assays 46)

Arginase Assay K755 CL, TL

Asparaginase Assay K754 CL, S, TL

Hydroxyproline Assay K555 S, TL, U

Serine Assay & more K743 CL, O, P, S, TL

Carbohydrates Assays (No. of assays 75)

Glucose Assay K606 O, P, S, U

Glucose Uptake Assay K676 CB

Glycogen Assay K646 CL, TL

Lactate Assay & more K607 CL, O

Coenzymes & Cofactors Assays (No. of assays 23)

ADP Assay K355 CL, TL

ADP/ATP Ratio Assay K255 CB

ATP Assay K354 CL, TL

Coenzyme A (CoA) Assay & more K367 CL, TL

Glycolysis Assays (No. of assays 55)

Glucose Assay K606 O, P, S, U

Glucose Assay II K686 O, P, S, U

Glucose Uptake Assay K676 CB

Lactate Assay & more K627 CL, S

Glyoxylate Assays (No. of assays 18)

α-Ketoglutarate Assay K677 TL

Glutathione Assay K261 CL, O, P, TL

Glyoxalase I Assay K591 CL, P, S, TL

Lactate Assay & more K667 CL, S, TL

Metabolism AssaysMetabolism is a vital process for all life forms. It is broadly defined as the sum of all the biochemical processes involved in maintaining the living state of the cells, and thus the organism. Metabolism plays a key role in disparate areas of physiology, cell biology, and medicine.

BioVision offers a wide range of Metabolism Assays. The table in the following pages illustrate the information on our most popular assays under various categories of Metabolism.


Popular Kits Cat. No. Sample TypeInorganic Ions Assays (No. of assays 23)

Calcium Assay K380 S, U

Iron Assay K390 CL, O, TL

Phosphate Assay K410 P, S

Zinc Assay & more K387 O, P, S, U

Intermediary Metabolism Assays (No. of assays 110)

Acetate Assay K658 CL, O, P, S, TL

Aconitase Assay K716 TL

Alcohol Dehydrogenase Assay K787 CL, TL

Amylase Assay & more K711 S, TL, U

Kidney Biomarker Assays (No. of assays 29)

Aldolase Assay K665 CL, P, S, TL

Carnitine Assay K642 CL

Creatinine Assay K625 O, S, U

Glucose Assay & more K666 CB

Kreb's Cycle Assays (No. of assays 21)

Citrate Assay K655 CL, TL

Citrate Synthase Assay K318 CL, TL

Pyruvate Assay K609 CL, O, S, TL

Succinate Assay & more K649 CL, O, TL

Lipid Metabolism Assays (No. of assays 58)

Cholesterol Efflux Assay K582 CB, PP, S

Free Fatty Acid Assay K612 CL, TL

Glycerol Assay K977 CB

Triglyceride Assay & more K622 CL, P, S, TL

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Figure 5: Glucose Colorimetric/Fluorometric Assay Kit (Cat. No. K606) Gluclose Standard Curve; (A) Colorimetric (B) Fluorometric, (C) Quantitation of Gluclose in human urine & serum. Urine & serum samples were deproteinized using a 10kDa Spin Column (10000xg, 10 min, 4 C). Urine filtrate (20 ul) & serum filtrate (1 ul) were spiked with a known amount of gluclose as internal standard (4nmol). Assays were performed according to the kit protocol. Calculated concentrations: Urine: 3.00 +/_ 0.4 mg/dl; Serum: 44.2 +/_ 6.7 mg/dl.

(A) (B) (C)

Click here for a complete list of Metabolism Assays.

(A) (B)

Figure 4: Hydroxyproline Colorimetric Assay Kit (Cat. No. K555) (A) Hydroxyproline Standard Curve. (B) Determination of hydroxyproline (Hyp) concentration in human urine. Briefly, samples were hydrolyzed with 12 N Hcl for 3 hrs at 120°C and clarified using activated charcoal. Activated charcoal and precipitates were removed by centrifugation (10000 x g, 3 min.). 10 µl of samples were spiked with 0.4 µg Hydroxyproline Standard. Samples were collected during different diet conditions. For an example, sample A was collected after strenuous exercise followed by fasting for 12 hrs. Sample B was collected 7 hours after sample A following normal uptake of food (900 calories). Assays were performed according to the kit protocol.

Popular Kits Cat. No. Sample TypeLiver Biomarker Assays (No. of assays 25)

Alanine Aminotransferase Assay K752 CL, S, TL

Cholesterol/Cholesteryl Ester Assay K603 CL, S, TL

GGT Activity Assay (C/F) K785, K784 CL, P, S

Lactate Dehydrogenase Assay & more K726 TL, CL

Oxidative Stress Assays (No. of assays 51)

Ethanol Assay K620 O

Glutathione (GSH/GSSG/Total) Assay K264 CL, S, TL

Lipid Peroxidation (MDA) Assay K739 CL, P, S, TL

TAC Assay & more K274 CL, S, U

Pentose Assays (No. of assays 10)

Glucose-6-Phosphate Assay K657 CL, O, TL

G6PDH Assay K757 CL, O, TL

G6PDH Assay (PicoProbe™) K687 CL, O, TL

6-PGDAssay & more K540 CL, TL

Serum Components Assays (No. of assays 50)

ATPase Assay K417 CL, TL

Bilirubin (Total and Direct) Assay K553 S

Ceruloplasmin K669 S

Creatine Kinase (CK) Assay & more K777 CL, P, S, TL

Popular Kits Cat. No. Sample TypeUrea Cycle Assays (No. of assays 31)

Ammonia Assay K370 CL, TL

Aspartate Aminotransferase Assay K753 S, TL

Aspartate Assay K552 CL, S, TL

Urea Assay & more K375 CL, O, P, S, TL, U

https://www.biovision.com/products/metabolism-assays/metabolism-assays-a-z.html

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Oxidative stress is a state when the normal reduced environment of a cell changes due to the accumulation of reactive oxygen species (ROS). BioVision has a number of kits to help researchers study and understand the detailed mechanisms of oxidative stress.

Oxidative Stress Assays

Product Name Cat. No. Sample TypeAscorbic Acid Assay Kit (C/F) K661 CL, S, TL, U

Total Antioxidant Capacity (TAC) Assay Kit (C) K274 CL, S, U

Catalase Activity Assay Kit (C/F) K773 CL, TL

DNA Damage Quantification Kit (C) K253 O

Ethanol Assay Kit (C/F) K620 O

Glutathione Assay Kit (C) K261 CL, O, P, TL

Glutathione Assay Kit (F) K251 CL, O, P, TL

Glutathione Assay Kit (GSH, GSSG and Total) (F) K264 CL, S, TL

Glutathione Detection Kit (Blue Fluorescence) (EZCell™) K504 CB

Glutathione Peroxidase Activity Assay Kit (C) K762 CL, TL

GST Activity Assay Kit (C) K263 CL, O, P, TL

GST Activity Assay Kit (F) K260 CL, O, P, TL

Intracellular Hydrogen Peroxide Detection Kit (CB) K204 CB

Lipid Peroxidation (MDA) Assay Kit (C/F) K739 CL, P, S, TL

Lipid Peroxidation Assay Kit (C) K454 S, P, TL

Myeloperoxidase (MPO) Activity Assay Kit (C) K744 CL, O, TL

Myeloperoxidase (MPO) Activity Assay Kit (F) K745 CL, O, TL

Myeloperoxidase (MPO) Inhibitor Screening Kit (F) K746 IC

Peroxidase Activity Assay Kit (C/F) K772 CL, O, P, S, U

Reactive Oxygen Species (ROS) Detection Assay Kit K936 CB

Reduced Glutathione (GSH) Assay Kit (F) (PicoProbe™) K740 CB, CL, P, TL

Superoxide Dismutase (SOD) Activity Assay Kit K335 CL, O, TL

Thioredoxin Reductase Activity Assay Kit (C) K763 CL, S, TL

Xanthine Oxidase Activity Assay Kit (C/F) K710 CL, S, TL


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Figure 6: Glutathione Peroxidase Activity Colorimetric Assay Kit (Cat. No. K762) (A) NADPH Standard Curve. (B) Measurement of GPx activity using purified enzyme. (C) GPx Activity was measured using rat liver lysate (23 ug), human serum (1 ul) and HeLa cell lysate (16 ug). Assays were performed following the kit protocol.

(A) (B) (C)

Click here for a complete list of Cell Damage and Oxidative Stress Assays.

Phosphatase & Protease Assays

Product Name Cat. No. Sample TypeAcid Phosphatase Activity Assay Kit (C) K411 CL, P, S, U

Acid Phosphatase Cell Cytotoxicity Assay Kit K937 CB

Alkaline Phosphatase Activity Assay Kit (C) K412 CL, P, S

Alkaline Phosphatase Activity Assay Kit (F) K422 CL, O, P, S, U

Caspase (1 - 10 & 12) Assay Kit (F/C) K110 - K119, K105, K106, K122 - K127 CL, TL

Caspase (1 - 10) Inhibitor Drug Screening Kit (F) K151 - K160 IC

Cathepsin (B/H/E/S/K/L/D) Inhibitor Screening Kit (F) K147 - K150, K161, K164, K166 IC

Cathepsin (E/B/K/L/D/S/H) Activity Assay Kit (F) K140 - K145, K165 CL, TL

Cathepsin G Activity Assay Kit (C) K146 CL, TL

Cathepsin G Inhibitor Screening Kit (C) K162 IC

Chymotrypsin Activity Assay Kit (F) K352 CL, P, TL

Factor IXa Activity Assay Kit (F) K364 P, PP, S

Factor VIIIa Activity Assay Kit (F) K358 P, PP, S(C)=Colorimetric | (CB)=Cell Based | (F)=Fluorometric | (FC)=Flow Cytometry

AB=Antibody | CB-Intact Cells | CL=Cell Lysate | IC=Inhibitor Compound | O=Other | P=Plasma | PP=Protein Preparation | S=Serum | TL=Tissue Lysate | U=Urine

Proteases control a variety of biological processes including apopotosis and angiogenesis. They are responsible in many activities like in regulation of localization, modification of protein-protein interactions, participate in processing of cellular information, and amplify molecular signals. They regulate the activation of many signaling proteins. Phosphorylation is a common post-translational modification of proteins that can be used for reversibly controlling or maintaining signal transduction cascades and physiology of cells.

https://www.biovision.com/products/cell-damage-oxidative-stress/assay-kits.html

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Product Name Cat. No. Sample TypeFactor Xa Activity Assay Kit (F) K361 P, PP

Factor Xa Inhibitor Screening Kit (F) K362 IC

Factor XIa Activity Assay Kit (C) K973 P, PP

Factor XIIa Activity Assay Kit (C) K994 P, PP

Factor XIIIa Activity Assay Kit (C) K522 P, PP

HIV-1 Protease Activity Assay Kit (F) K825 NA

HIV-1 Protease Inhibitor Screening Kit (F) K826 NA

HIV-2 Protease Activity Assay Kit (F) K845 NA

HRV 3C Protease Activity Assay Kit (C) K214 NA

MMP-(1/3/9/14) Inhibitor Screening Kit (F) K794, K793, K844, K846 IC

MMP-3 Activity Assay Kit (F) K783 CL, TL

Neutrophil Elastase Activity Assay Kit (F) K383 O, P, PP

Neutrophil Elastase Inhibitor Screening Kit (F) K782 IC

Phosphate Assay Kit (F) K420 O, S

Protease Activity Assay Kit (F) K781 NA

Proteasome Activity Fluorometric Assay Kit K245 CL

TEV Protease Activity Assay Kit (F) K842 NA

Thrombin Activity Assay Kit (F) K373 P, PP

Thrombin Cleavage Kit K377 PP

Thrombin Inhibitor Screening Kit (F) K374 IC

Trypsin Activity Assay Kit (C) K771 CL, TL

Figure 7: Factor XIIa Activity Assay Kit (Colorimetric) (Cat. No. K994): Kinetic progressive curves for different amounts of FXIIa Enzyme (A) and Activated Plasma Samples (B) are shown. Standard curve for pNA (n = 3) (C) was used to estimate FXIIa activity in Normal Pooled Human Plasma and FXII-deficient Human Plasma (n = 3) (D). Assays were performed according to the kit protocol.

Click here for a complete list of Phosphatase Assays and click here for a complete list of Protease Assays.

(A)

(C)

(B)

(D)

https://www.biovision.com/catalogsearch/result/index/?product_type=16&q=phosphatase

https://www.biovision.com/catalogsearch/result/index/?product_type=16&q=protease

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Cell-Based AssaysBioVision is proud to offer an expanding selection of assays to study some fundamental processes governing cellular biology. Assays to study different aspects of cellular behavior (progression through the cell cycle, migration, invasion) and measure the content of key building blocks in the cell are commonly used to monitor the health of cells in culture and their response to various stimuli (migration inducers, cell cycle arrest drugs, cholesterol transport inhibitors, etc.).

Product Name Cat. No. Sample TypeCell Viability and Proliferation Assays

Autophagy/Cytotoxicity Dual Staining Kit (CB) K449 CB

BrdU Cell Proliferation Assay Kit K306 CB

Calcein AM Cell Viability Assay Kit (F) (EZViable™) K305 CB

3D Cell Culture HTS Cell Viability Complete Assay Kit K948 CB

Cell Proliferation Assay Kit (F) K307 CB

Cell Viability Assay Kit (ApoSENSOR™) K254 CB

MTS Cell Proliferation Assay Kit (C) K300 CB

MTT Cell Proliferation Assay Kit (C) K299 CB

Quick Cell Proliferation Assay Kit & Assay Kit Plus (C) K301 CB

Quick Cell Viability Assay Kit (F) (VisionBlue™) K303 CB

Cell Invasion and Migration Assays

Cell Invasion Assay (Collagen IV), 12-well, 8 µm (EZCell™) K919 CB

Cell Invasion Assay Kit (Basement Membrane), 12-well, 8 µm (EZCell™) K913 CB

Cell Invasion Assay Kit (Collagen I), 12-well, 8 µm (EZCell™) K917 CB

Cell Invasion Assay Kit (Laminin), 24-well, 8 µm (EZCell™) K915 CB

Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) (EZCell™) K909 CB




EZClick Assays

TUNEL - in situ DNA Fragmentation/Apoptosis Assay Kit (EZClick™) K191 CB, CL, TL

EdU Cell Proliferation/DNA Synthesis Kit (EZClick™) K946 CB

Fucose Modified Glycoprotein Assay Kit (EZClick™) K592 CB

Global Phospholipid Synthesis Assay Kit (EZClick™) K717 CB

Global Protein Synthesis Assay Kit (EZClick™) K467 CB


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Product Name Cat. No. Sample TypeGlobal RNA Synthesis Assay Kit (EZClick™) K718 CB

Global RNA Synthesis Assay Kit (EZClick™) K462 CB

Global RNA Synthesis Assay Kit (EZClick™) K497 CB

O-GalNAc Modified Glycoprotein Assay Kit (EZClick™) K560 CB

Palmitoylated Protein Assay Kit (EZClick™) K416 CB

Total Phospholipid Assay Kit (CB) (EZClick™) K537 CB

Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (EZClick™) K441 CB

Stearoylated Protein Assay Kit (EZClick™) K453 CB

Click here for a complete list of Cell Based Assay Kits.

Cell Apoptosis AssaysApoptosis, or programmed cell death, characterized by nuclear condensation, cell shrinkage, membrane blebbing, and DNA fragmentation, plays an important role in many physiological and diseased conditions. BioVision has developed various assays and products that can detect apoptosis at the early, middle, and late stages in an apoptotic cascade.


(A) (B)

Figure 8: EZClick™ Global RNA Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence (K718) Analysis of RNA biosynthesis in presence of Actinomycin D. (A) Jurkat cells (1X106 cells/ml) were pre-treated with vehicle (black line) or 1 X Actinomycin D (blue line) for 4 hours at 37°C prior to 1 hour incubation with EZClick™ RNA Label (red line). Cells were then processed for detection of de novo synthesized RNA according to the included protocol. Fluorescence measured in FL-2 channel clearly shows the inhibitory effect of Actinomycin D on RNA synthesis. (B) HeLa cells (105 cells/ ml) were pre-treated either with vehicle (top) or 1 Actinomycin D (bottom) for 4 hours at 37°C prior to 1 hour incubation with EZClick ™ RNA Label. De novo synthesized RNA was detected by Fluorescence Microscope. Reduced red fluorescence in panel B confirms the inhibitory effect of Actinomycin D on RNA biosynthesis. Nuclear staining in both panels confirms that red fluorescence is the result of EZClick™ RNA Label incorporation.

https://www.biovision.com/products/cell-based-assays.html/

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Click here for a complete list of Cell Apoptosis Assays.

Figure 9: DNAse I Activity Assay Kit (Fluorometric) (Cat. No.K429) (A) DNA Probe to Product conversion standard curve; (B) representative activity curve for purified DNAse I (orange), serum sample (green), and background control (blue) at 37°C; (C) comparative analysis of DNAse I activity from 25 µl undiluted single donor normal vs. Systematic Lupus Erythematosus (SLE) patient serum sample.

(A) (B) (C)

Product Name Cat. No. Sample TypeADP/ATP Ratio Bioluminescent Assay Kit (ApoSENSOR™) K255 CB, CL

Annexin V- (Cy3, FITC, or PE) Apoptosis Detection Kit Plus K201 CB

Annexin V-Cy3 Apoptosis Kit K102 CB

Annexin V-Cy3 Apoptosis Kit Plus K202 CB

Annexin V-Cy5 Apoptosis Kit K103 CB

Annexin V-EGFP Apoptosis Kit K104 CB

Annexin V-FITC Apoptosis Kit K101 CB

Annexin V-PE Apoptosis Detection Kit K128 CB

Annexin V-PE Apoptosis Kit Plus K203 CB

Annexin V-PE-Cy5 Apoptosis Detection Kit K129 CB

Caspase Staining Kit (CaspGLOW™ Fluorescein Active) K180 CB

Caspase Staining Kit (CaspGLOW™ Red Active) K190 CB

Caspase-2 Staining Kit (CaspGLOW™ Fluorescein Active) K182 CB

Caspase-3 Inhibitor Drug Screening Kit (F) K153 IC





Cytochrome c Releasing Apoptosis Assay Kit K257 CB

Cytochrome Oxidase Activity Assay Kit (C) K287 CB, CL, TL

DNA Fragmentation Assay Kit (ApoBrdU) K401 CB

DNA Fragmentation Assay Kit (ApoBrdU-IHC) K403 CB

In Situ DNA Fragmentation Assay Kit (ApoDIRECT) K402 CB

In Situ DNA Fragmentation Assay Kit (Apo-BrdU-Red) K404 CB

Enhanced Apoptotic DNA Ladder Detection Kit K130 CB

Mitochondrial Apoptosis Detection Kit, MitoCapture (F) K250 CB

Quick Apoptotic DNA Ladder Detection Kit K120 CB


https://www.biovision.com/catalogsearch/result/index/?product_type=16&q=Apoptosis

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Click here for a complete list of EZScreen™ 384-well Assays.

EZScreen™ 384-well AssaysEZScreen™ 384-well colorimetric assays generate stable chromophores that are directly proportional to the analyte of interest. This method is quantitative, rapid, simple, sensitive, and designed to be used in high throughput setting. The following table includes all of the EZScreen™ 384-well assays from BioVision.

Product Name Cat. No. Sample TypeATP Assay Kit (384-well) (C) (EZScreen™) K959 CL, TL

Free Fatty Acid Assay Kit (384-well) (C) (EZScreen™) K956 CL, O, P, S, TL

Glucose Assay Kit (384-well) (C) (EZScreen™) K950 S

Glycogen Assay Kit (384-well) (C) (EZScreen™) K960 TL, CL

β-Lactamase Activity Assay Kit (384-well) (C) (EZScreen™) K954 O

Lactate Assay Kit (384-well) Format (C) (EZScreen™) K951 CL, O, P, S

Lactate Dehydrogenase Activity Assay Kit (384-well) (C) (EZScreen™) K953 O, P, S, TL

NAD+ /NADH Assay Kit (384-well) (C) (EZScreen™) K958 CL, P, S, TL

Total Cholesterol & Cholesteryl Ester Assay Kit (384-well) (C) (EZScreen™) K957 CL, S, TL

Triglyceride Quantification Assay Kit (384-well) (C) (EZScreen™) K952 CL, P, S, TL

Figure 10: EZScreen™ Free Fatty Acid Colorimetric Assay Kit (384-well) (Cat. No. K956) (A) Palmitic Acid Standard Curve. (B) Determination of Free Fatty Acid in pooled normal human serum. Undiluted serum sample (2 µl, untreated) was assayed directly according to the kit protocol. Calculated Free Fatty Acid concentration: 312 ± 4.2 μM.

(A) (B)


https://www.biovision.com/catalogsearch/result/?q=EZScreen%E2%84%A2

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Inhibitor Screening Assays

Product Name Cat. No. Sample TypeAHCY Inhibitor Screening Kit (F) K326 IC

Carbonic Anhydrase (CA) Inhibitor Screening Kit (C) K473 IC

Cathepsin B (CTSB) Inhibitor Screening Kit (F) K147 IC

Cathepsin K Inhibitor Screening Kit (F) K150 IC

Cathepsin L Inhibitor Screening Kit (F) K161 IC

CETP Inhibitor Screening Kit II (F) K594 IC

COX-2 Inhibitor Screening Kit (F) K547 IC

Cyclooxygenase-1 (COX-1) Inhibitor Screening Kit (F) K548 IC

DPP4 Inhibitor Screening Kit (F) K780 IC

Granzyme B Inhibitor Screening Kit (F) K169 IC

HDAC-5 Inhibitor Screening Kit (F) K171 IC

HDAC8 Inhibitor Screening Kit (F) K368 IC

HMG-CoA Reductase Activity/Inhibitor Screening Kit (C) K588 IC

MMP-1 Inhibitor Screening Kit (F) K794 IC

Monoamine Oxidase A & B (MAO-A & B) Inhibitor Screening Kit (F) K796, K797 IC

Neutrophil Elastase Inhibitor Screening Kit (F) K782 IC

p300 Inhibitor Screening Kit (F) K346 IC

pCAF Inhibitor Screening Kit (F) K345 IC

Plasma Kallikrein Inhibitor Screening Kit (C) K989 IC

Plasmin Inhibitor Screening Kit (F) K382 IC(C)=Colorimetric | (CB)=Cell Based | (F)=Fluorometric | (FC)=Flow Cytometry

AB=Antibody | CB-Intact Cells | CL=Cell Lysate | IC=Inhibitor Compound | O=Other | P=Plasma | PP=Protein Preparation | S=Serum | TL=Tissue Lysate | U=Urine

Click here for a complete list of Inhibitor Screening Assays.

Figure 11: (A) p300 Inhibitor Screening Assay Kit (Fluorometric) (Cat. No. K346) Inhibition of p300 activity by the p300 Inhibitor [Anacardic Acid] (Cat. No. 1849). Assays were performed following the kit protocol. (B) COX-2 Inhibitor Screening Kit (Fluorometric) (Cat. No. K547) Inhibition of COX-2 Activity with Celecoxib. IC50 of Celecoxib was determined to be 0.45 μM. Assay was performed following the kit protocol.

(A) (B)

BioVision's inhibitor screening kits provide sensitive, quantitative readouts to test and refine your inhibitor candidates.

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Product Name Cat. No.Acetate K658Acetoacetate K650Acetylcholinesterase K197, K764Acetyl-CoA (PicoProbe™) K317N-Acetylglucosaminidase K733Acid Phosphatase K411, K421, K937Acid Sphingomyelinase K192Acidic Chitinase K513, K693Aconitase K716Adenosine K327Adenosine Deaminase (ADA) K321, K328, K993Adenosylhomocysteinase (AHCY) K326, K807Adenylate Kinase (AK) K350Adipogenesis K610ADP (PicoProbe™) K211 & moreAdvanced Glycation End Products (AGEs) K929Akt K435Alanine K652Alanine Aminotransferase (ALT or SGPT) (EZScreen™) K941 & moreAlbumin (Albuminuria) K550Albumin (BCG) K554Albumin-to-Creatinine Ratio (ACR) K551Alcohol Dehydrogenase K787Aldehyde Dehydrogenase (PicoProbe™) K741Aldo-keto Reductase (EZDetect™) K847Aldolase K665Aldose Reductase K174, K369Alkaline Phosphatase K412, K422Alkaline Sphingomyelinase K987Amino Acids K445, K559, K639Aminopeptidase N K523Ammonia K370, K470Amylase K482, K711β Amyloid 1- 42 (Aβ42) Ligand K570Angiogenesis (Tube Formation) K905Angiotensin I Converting Enzyme (ACE1) K227, K228Angiotensin II Converting Enzyme (ACE2) K310, K8971,5-Anhydroglucitol Uptake K684Annexin v K101, K102, K103 & moreArginase K567, K755Arginine K384, K749Aromatase K983, K984Ascorbic Acid K661, K671Asparaginase K754Asparagine K736Aspartate K552Aspartate Aminotransferase K753ATP (EZScreen™) K959 & moreATPase K417Total Bile Acids (TBA) K209Bilirubin (Total and Direct) K553Branched Chain Amino Acid (Leu/Ile/Val) K564Butyrylcholinesterase K516Calcium (EZCell™) K432 & moreCalpain K240, K244cAMP K371Carbohydrate K645Carbonic Anhydrase K472, K473Cardiolipin K944

Product Name Cat. No.Carnitine K642Caspase-1 K110, K111, K151Caspase-2 K116, K117, K152Caspase-3 K105, K106, K533Caspase-4 K126, K127, K154Caspase-5 K122, K123, K155Caspase-6 K114, K115, K156Caspase-7 K157Caspase-8 K112, K113, K158Caspase-9 K118, K119, K159Caspase-10 K124, K125, K160Caspase-12 K139Catalase K773Cathepsin B K140, K147Cathepsin D K143, K148Cathepsin E K165, K166Cathepsin G K146, K162Cathepsin H K145, K164Cathepsin K K141, K150Cathepsin L K142, K161Cathepsin S K144, K149Cell Cycle (EZCell™) K920Cell Invasion (EZCell™) K912, K913, K917 & moreCell Migration (EZCell™) K906, K911, K908 & moreCell Proliferation K299, K300, K304 & moreCell Viability (ApoSENSOR™/EZViable™/VisionBlue™) K254, K303, K305 & moreCell-Mediated Cytotoxicity K315Ceruloplasmin K669CETP K594, K595cGMP K372Chitotriosidase K512, K513Chloride K530Cholesterol & Cholesteryl Ester (EZScreen™) K957Cholesterol Efflux K582Choline K476, K615Cholinesterase K975Chymotrypsin K352Citrate K655Citrate Synthase K318Cobalt K505Coenzyme A (CoA) K367Collagen K107, K148, K169 & moreCollagenase K490, K792, K833Copper K899COX 1 & 2 K547, K548, K549Creatine K635Creatine Kinase (CK) K777Creatinine K625CYP1A2 K893CYP2C19 K848, K849CYP2C9 K895, K896CYP2D6 K703, K704CYP3A4 K701, K702Cystathionine β Synthase K695, K998Cysteine K558Cytidine Deaminase K451Cytochrome Oxidase K287Cytochrome P450 Reductase K700Cytotoxicity K311, K313, K314

Target List


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Product Name Cat. No.Deubiquitinase K485Diamine Oxidase K496Dihydrofolate Reductase K246, K247Dihydroxyacetone Phosphate (DHAP) (PicoProbe™) K673DNA Damage K253DPP4 K779, K780Endothelin Converting Enzyme 1 K536Enolase K691Enteropeptidase/Enterokinase K758, K759Ethanol K526, K620Factor IXa K364Factor VIIIa K358Factor Xa K361, K362Factor XIa K973Factor XIIa K994Factor XIIIa K522FAD K357Fatty Acid Amide Hydrolase (FAAH) K434Fatty Acid Uptake K408Ferric Reducing Antioxidant Power (FRAP) K515Formaldehyde (PicoProbe™) K805Formate K653Free Fatty Acid (EZScreen™) K956Free Glycerol (PicoProbe™) K643 & moreFructosamine K450Fructose (PicoProbe™) K611 & moreFructose-1,6-Bisphosphatase (PicoProbe™) K399 & moreFructose-6-Phosphate (PicoProbe™) K689Fucosidase K224, K542Fumarase K596Fumarate K633Galactose K621Galactose and Lactose K617α Galactosidase (α-Gal) K407β Galactosidase (β-Gal) K821GAPDH K680Gelatinase (Gelatin Degradation/Zymography) K444Glucokinase (PicoProbe™) K969Gluconate (D-Gluconic Acid) K683Gluconokinase (GntK) K319Glucose (EZScreen™/PicoProbe™) K688 & moreGlucose and Sucrose K616Glucose Isomerase K491Glucose Oxidase K788Glucose Uptake (EZCell™/GluTracker™) K681 & moreGlucose-1-Phosphate K697Glucose-6-Phosphate (PicoProbe™) K751 & moreGlucosidase K690, K938Glucuronidase K514Glutamate (PicoProbe™) K413 & moreGlutamate Carboxypeptidase II (PicoProbe™) K738 & moreGlutamate Dehydrogenase K185, K729Glutaminase (GLS) (PicoProbe™) K455 & moreGlutamine K556γ Glutamyl Transferase (GGT) K784, K785Glutathione K251, K261, K762 & moreGlycerol K977Glycerol-3-Phosphate (PicoProbe™) K196 & moreGlycerophosphorylcholine K433Glycine K589Glycogen (EZScreen™) K960 & more

Product Name Cat. No.Glyoxalase I K460, K591Granzyme B K168, K169GST K260, K263HAT K332, K334, K336HDACs K330, K331, K466 & moreHDL and LDL/VLDL K613HDL Uptake K586Heme K672Hemoglobin K219Hepatic Steatosis K584Hexokinase (PicoProbe™) K769 & moreHexokinase (HK) K713, K828Histamine K386, K506Histone Acetyltransferase K334, K336HIV-1 & 2 K825, K826, K845HMG-CoA K588Homocysteine K531HRV 3C K214, K215Hydrogen Peroxide K204, K26515-Hydroxy prostaglandin dehydrogenase (PicoProbe™) K562Hydroxybutyrate (PicoProbe™) K651 & more2-Hydroxyglutarate (D2HG) (PicoProbe™) K248 & moreHydroxyproline K226, K555IDH1 R132H Mutant K493IMPDH K495Indoleamine 2,3-Dioxygenase 1 K972, K976Inosine K712Inulin (PicoProbe™) K737Invertase K674Iron K390Total Iron-Binding Capacity and Serum Iron K392Isocitrate K656, K756, K985Isopropanol Dehydrogenase K698JNK K430, K431Kallikrein K989, K997α-Ketoglutarate K677, K678β-Lactamase Activity (EZScreen™) K954 & moreLactate K627, K667, K726 & moreLactose K624Lactulose (PicoProbe™) K662Leucine Aminopeptidase K534Lipase K722, K723, K724Lipid K216, K580Lipid Peroxidation K454, K739Lipolysis (PicoProbe™) K578 & moreLipoprotein Lipase K721Lipoxygenase K978, K980Lithium K545Luciferase K801Lysosomal α-Glucosidase (GAA) K187Lysosome K235, K448, K458Lysyl Oxidase K928Magnesium K385Malate (PicoProbe™) K538 & moreMaltose and Glucose K618Mannitol K644Matrix Metalloproteinase (MMP) K783, K793, K794 & moreMethanol K898Methionine K442Methylglyoxal (PicoProbe™) K461Methyltransferase K521, K822, K986

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Product Name Cat. No.Mitochondria Complex 1, 3 K520, K968Mitochondria Permeability Transition Pore K239Mitochondrial DNA K280Mitochondrial Membrane Protential K238Monoacylglycerol Lipase K474, K561Monoamine Oxidase (Total MAO/MAO-A/MAO-B) K795, K796, K797Multidrug Efflux Transporter (MDR) K507Myeloperoxidase (MPO) K744, K745, K746, K747myo-Inositol (PicoProbe™) K469NAD/NADH/NADPH (EZScreen™/PicoProbe™) K338, K958 & moreNAFLD K489Neprilysin K487, K996Neuraminidase K524, K732Neutrophil Elastase K383, K782Nickel K510Nicotinamide Methyltransferase K822Nitric Oxide K205, K252, K262 & moreN'-Nicotinamide Methyltransferase (NNMT) K822Non-Alcoholic Fatty Liver Disease (NAFLD) K4895’-Nucleotidase (CD73) K992Ornithine K176, K939Oxalate (Oxalic Acid) K509, K663, K664Oxaloacetate K659p300 K346p53 K923, K961, K966PAF Acetylhydrolase K765, K766PAH (p-Aminohippuric Acid) K860Palmitoylated Proteins (EZClick™) K416, K452Paraoxonase 1 K999pCAF K345PEG Virus K904PEP K365Peroxidase K772Phenolic Compounds K527Phenylalanine K481, K572Phosphate (PicoProbe™) K419 & morePhosphatidylcholine K576Phosphodiesterase K927Phosphoenolpyruvate K359, K443Phospho-ERK1/2 (Thr202/Tyr204) K696Phosphofructokinase (PFK) K776Phosphoglucomutase (PicoProbe™) K770 & morePhosphogluconate Dehydrogenase K4886-Phosphogluconate Dehydrogenase K5406-Phosphogluconic Acid (6-PGA) K217Phosphoglucose Isomerase K775Phosphoglycerate K194, K569, K778Phospholipase K400, K725Phospholipid K351Phospho-p38 MAPK (Thr180+Tyr182) K965Plant Tissue K296Plasma Kallikrein K997PLTP K593, K605Total Polyamine K475PON1 K999Potassium (Serum) K940PPARγ K437Preadipocyte Isolation K583Protease K781, K841, K843 & moreProtein Carbonyl Content K563, K830

Product Name Cat. No.Protein Disulfide Isomerase K840, K949Protein Labeling (EZLabel™) K832, K835, K837, K839Protein Tyrosine Phosphatase K829PSA K962Purine Nucleoside Phosphorylase K767, K768Pyrophosphate (PPi) K568Pyruvate K456, K609, K679, K709Pyruvate Dehydrogenase K679Pyruvate Kinase K709Reactive Oxygen Species (ROS) K936Reduced Glutathione (GSH) (PicoProbe™) K740 & moreRenin K798, K799, K800, K806RNA Synthesis (EZClick™) K462, K718Sarcosine K636Secretase K360, K388, K720Senescence K320, K991Serine K743Sialic Acid (NANA) K566SIRT K323, K325Sirtuin K324Sodium K391Soluble Epoxide Hydrolase K477, K480Sorbitol K631, K935Sphingomyelin K600Sphingomyelinase K574, K599Starch K647Stearoylated Protein (EZClick™) K418, K453Succinate K649, K660Succinyl-CoA Synthetase K597Sucrose K626Sulfate K415Total Sulfite K699Superoxide Dismutase (SOD) K3353T3-L1 K579TAC K274TACE K366Taurine K988TEV Protease K842, K843Thioredoxin K535, K763Threonine (PicoProbe™) K463Thrombin K373TMRE K238Transglutaminase K508, K571Triglyceride (EZScreen™/PicoProbe™) K614, K952 & moreTriose Phosphate Isomerase (TPI) K670Trypsin K771Tryptophan K557Tyrosinase K575, K742Tyrosine K573Tyrosine Phosphatase K829UGT K692Universal Kinase (ADPsensor™) K212Urea K375, K376Urease K378Uric Acid K608Uricase K734Urokinase Activity K727, K728Xanthine K685, K710Xylosidase K981Zinc (EZCell™) K525 & more

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