custom dna oligonucleotide synthesis services

The world leader in serving science

Custom DNA Oligonucleotide Synthesis Services

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invested in R&D$1B

R&D scientists/engineers5,000

employees>75,000

in revenue>$25B

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DNA Synthesis

Capabilities

Security of supply

ISO 9001 and ISO 13485 QMS

Access to manufacturing resources and expertise

CustomizationWorkflow solutions

Commercial supply process development

Partnership

DNA Synthesis Capabilities – Bringing Value to Our Partners

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At Thermo Fisher Scientific, we have over 25 years of experience in providing a complete range of custom-synthesized oligonucleotide primers, probes, and genes, all built to your specifications. With trusted Applied Biosystems™ TaqMan® Assays and innovative Invitrogen™ GeneArt™ Gene Synthesis services, you can expect quality, reliability, and convenience.

Capability Our Value

• Natural DNA

• End modifications

• Base modifications

• Aptamers

• Large-scale synthesis

• Custom developed modifications

• Unique process development and methods

• Specialty project teams for large scale or volume needs

• Consultation, partnership, and expertise

• Scale and resources of Thermo Fisher Scientific

DNA Oligonucleotide Product and Service Offering

A solid worldwide infrastructure helps ensure on-time delivery, long-term sustainability, and superior service and support. Our team works with you on every step of your research journey.

5 Proprietary & Confidential

25 years of experience in oligo synthesis with global manufacturing footprint and infrastructure to fulfill your needs for any application. Choose from a wide range of purification scales, synthesis scales, and delivery formats to meet your specific needs.

DNA Oligonucleotide Capability

Tube/ plate Primer length (bases) 25 nmol 50 nmol 200 nmol 1 µmol 10 µmol

Desalted 10-100 ● ● ● ● ●

Cartridge ≥75% purity 7-55 ● ● ● ●

HPLC ≥ 85% purity 10-55 ● ● ● ●

PAGE ≥ 85% purity 7-100 ● ● ● ●

Large scale synthesisSpecialty project teams for transition to large scale production for true end-to-end service: • 5 mg to multigram

• Process development for kg

• Purification methods to achieve ≥ 85% purity

• Analytical QC, QC testing for residual solvents, endotoxins and microbial enumeration

• ISO 9001, ISO 13485 Compliance


Modification and Purification Options Available

Purification method Description Benefit

Desalt(25 nm: 10–100 nt;50 nm: 5–100 nt)

Oligos are processed through normal phase chromatography column which removes salts but not failure sequences.

A salt-free DNA solution, ready-to-use; suitable for many PCR and sequencing applications without further purification.

Cartridge(50 nm–1 µm, 7–55 nt)

Based on reverse phase chromatography; removes failure sequences from the completed synthesis.

Provides full-length sequences needed in some applications.

HPLC(50 nm+, 10–55 nt)*Long oligo HPLC available

Reverse Phase High Performance Liquid Chromatography (HPLC) removes failure sequences or unincorporated label the same way as cartridge purification.

Guarantees highly purified primer required in some applications (>=85% full length).

PAGE(200 nm+, 7–100 nt)

Method used to differentiate full-length product from failure sequences based on size and conformation.

Provides the highest percentage of full-length oligos (>=85%) required for certain demanding applications such as mutagenesis or adapter production.

Modification Options: Generic linkers for attachment chemistry, fluorescent dyes, molecular probe dyes, alternative internal bases, phosphorothioates, mixed bases, spacers, phosphorylation, biotinylation, and custom modification development

Purification Options: Standard desalting, Cartridge, HPLC, and PAGE. For large scale synthesis and specialty oligo projects, RP and Ion Exchange HPLC is available


Recommended Purity by Application

Application Desalted (baseline) Cartridge ≥ 75% purity HPLC ≥ 85% purity PAGE ≥ 85% purity

PCR ●

PCR using oligos with critical 5’ sequences

● ●

Cloning adapters ● ●

NGS adapters ● ●

Fluorescent sequencing ●

First-strand cDNA synthesis for generation of libraries

● ●

Microarrays ●

AFLP analysis ●

Antisense ●

Gel shift assays ● ●

Isothermal sequencing ●

Site-directed mutagenesis ● ●


Modification Options AvailableThe table below shows the most common modifications available. If the modification you are looking for is not listed, our customer service team will work with you to address any unique requirements. Email us at [email protected]

Chemical Modification 3’ 5’ Internal 25 nmol 50 nmol 200 nmol 1 µmol 10 µmol

Aldehyde ● ● ● ●

Alexa Fluor 350* ● ● ● ●















Alexa Fluor 750* ● ● ● ●* These product require HPLC purification




Amino/amine ● ● ● ● ● ●

Amino C12 ● ● ● ●

Biotin ● ● ● ● ● ● ●

Biotin TEG ● ● ● ●

BODIPY 530/550 ● ● ● ●

BODIPY 581/591 ● ● ● ●

BODIPY FL ● ● ● ●

BODIPY FL-X ● ● ● ●

BODIPY R6G ● ● ● ●

BODIPY R6G-X ● ● ● ●

BODIPY 630/650 ● ● ● ●

BODIPY 493/503 ● ● ● ●

BODIPY 558/569 ● ● ● ●

BODIPY 564/570 ● ● ● ●

BODIPY 576/589 ● ● ● ●

BODIPY 650/660 ● ● ● ●

BODIPY TMR ● ● ● ●




BODIPY TR-X ● ● ● ●

Cascade Blue ● ● ● ●

DeoxyInosine ● ● ● ● ●

Deoxyuracil ● ● ● ● ●

FAM ● ● ● ● ●

Fluorescein ● ● ● ●

Gateway forward ● ● ● ●

Gateway reverse ● ● ● ●

HEX ● ● ● ●

Marina Blue ● ● ● ●

Oregon Green 488 ● ● ● ●

Oregon Green 488-X ● ● ● ●

Oregon Green 514 ● ● ● ●

Phosphorothioate ● ● ● ● ● ● ●

Pacific Blue ● ● ● ●

Pacific Orange ● ● ● ●

Phosphate ● ● ● ● ● ● ●




Rhodal Green ● ● ● ●

Rhodamine Green-X ● ● ● ●

Rhodamine Red-X ● ● ● ●

ROX* ● ● ● ● ●

TAMRA* ● ● ● ● ●

TET ● ● ● ● ●

Texas Red-X ● ● ● ●

Thiol ● ● ● ● ●

* These product require HPLC purification


Delivery Lead Times

Tube Delivery Schedule *Carrier transit times may impact delivery date

Type of oligonucleotide Ships within Delivery on*

Desalted, unmodified, ≤ 45 bases ordered before 5 pm Easter Time (US) 1 business day 2nd business day

Desalted, 46-100 bases, phosphorylated, phosphorothioate modifications, cartridge purification

2 business days 3rd business day

3’ and 5’ modifications 5 business days 6th business day

HPLC and PAGE purifications, 1 and 10 µmol scales 6 business days 7th business day

Plate Delivery Schedule

Type of oligonucleotide Ships within Delivery on*1-4 plates 3 business day 4th business day

5-8 plates 4 business days 5th business day

9-14 plates 6 business days 7th business day

15-35 plates 8-10 business days 9th business day+

36+ Inquire

Domestic Shipping:• Standard, overnight shipping• Dried oligo, ambient Monday through Saturday• Resuspended oligo, frozen Monday through Thursday


Ordering Options

Easy, fast and simple online ordering portal:

Our intuitive Invitrogen™ ordering portal enables you to order multiple oligos on the same page with a batch editing function, dynamic selection menus, and real-time shipping and price updates.

Easy order functions are available for bulk uploads:• Upload single, multiple of bulk requests up to 200 oligos • Save designs for quick reorder

Order from thermofisher.com/oligo

Free, online primer design tool We offer a free Primer3-based, online primer designer for your PCR, CE sequencing, and cloning success. • Save time when designing your primers – up to 50 genes at the same time for PCR or CE design• Store your data – ability to save your projects • Worker smarter – recognizes .txt and .fasta file types • Order with ease – seamless integration with the ordering portal

Try OligoPerfect™ 2.0 primer designer at thermofisher.com/oligoperfect-designer


Global Manufacturing, Support, and Service

Well-established global manufacturing footprint and infrastructure

• Multiple levels of regulated manufacturing and advanced quality systems – ISO 9001 and ISO 13485

• Center for Oligo Excellence – Pleasanton, CA, USA

• Consistent product performance

• Dedicated OEM and contract manufacturing if needed

Pleasanton, CA

Beijing, Suzhou,

Guangzhou, China

Inchinnan, UK

Sao Paulo, Brazil

Haneda, Japan

Auckland, New Zealand

Technical and Customer Service teams worldwide


For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals. © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. COL111873 0720

custom dna oligonucleotide synthesis services

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