Your Peptides International Custom Synthesis Team LeadersTwo key advantages, support and expertise, are exemplified by the custom peptide team backing your project. These specialists

have decades of cumulative knowledge, combined with a sense of ownership and dedication to each custom project.

Production

Michael Pennington, Ph.D.President, Chief Executive Officer

Dr.Penningtonhasover25yearsinresearchanddevelopmentmanagement.Hecontinuestoactivelypublishwithmorethan115publications.Hisactiveprojectinvolvementincomplexpeptidesespeciallythosewithmultipledisulfidebondskeepshisfingersonthepulseofthecompany.Hehasservedoneditorialboardsoffourpeptidesciencerelatedjournalsandparticipatesonthescientificadvisoryboardsofseveralbiotechcompanies.

Dr. Satendra Chauhan, Ph.D.Chief Scientific Officer

Dr.Chauhanhasextensiveexpertiseinretrosyntheticanalysis,synthesis,purificationandanalysisofcomplexorganic molecules, peptides andpeptidomimetics as well as totalsynthesis of natural products. Dr.Chauhanhasoverthreedecadesofpeptidelaboratoryexperience.

Andrzej Czerwinski, Ph.D.Senior Chemist

Dr. Czerwinski’s career in peptidechemistryspansover40yearsandhe hasbeena vital PImember forwelloveradecade.Withnumerouspeer-reviewed papers and articlesto his credit,Dr.Czerwinski bringsliterallyalifetimeofexperienceandinsighttoeachproject.

Rosendo Estrada, Ph.D.Production Manager

Dr. Estrada oversees all of theproductioneffortsinthesolid-phaselaboratories. He manages ourproductionchemists fromthesmallscale up through the pilot scaleproduction campaigns which areconductedatPI.Heutilizesourvastarray of state-of-the-art equipmenttoquicklyandefficientlysynthesizeyourproducts.

Vikas Dharwan Ph.D.Purification Manager

Dr.Dhawanhasvastexperienceinboth research peptide purificationas well as commercial drugproduction under cGMP. Vikasbrings this wealth of knowledge tohisteamtoexpertlypurifypeptidesto the most exacting standardswhichyoumayrequest.

Quality Assurance Quality Control Customer Care

Patricia Coxon Ph.D. Director of Quality Management SystemsDr. Coxon assures a strict adherance toquality standards while expediting smoothproject management. She serves as liasonbetween the customer and PI laboratory,securing a stress-free realization for eachcustom project, and that the ISO 9001standardsaremet.

Yingjun Zhang, M.S.Quality Control Manager

YingjunZhangisthefinalauthorityforqualitycontrol,certifyingthatthedatamatchestherequested specifications, for each custompeptideproducedinthePIlaboratory.

Timothy Morgan, M.S.Director of Sales

Withextensiveexperienceineveryaspectof Peptides International’s laboratories,Timothy Morgan is uniquely qualified toshepherd your project throughout theprocesssinaneconomicalandexpeditedmanner,withtheperspectiveofsomeonewhotrulyknows“howit’sdone.”

Custom Peptide & Chemistry Services

Certified to ISO 9001:2008

Peptides International has made a major commitment in personnel and equipment that significantly increases the breadth of its technology in peptide science. These resources, together with the company’s broad background in solving custom peptide challenges , now offers researchers a COMPLEX CUSTOM PEPTIDE TECHNOLOGY PLATFORM from which to develop new projects. The platform assures an integrated company-wide effort that not only meets researcher’s immediate synthetic needs, but can offer additional services in areas such as analytical testing, technology/process development, and scale-up. Examples of several elements of the platform are described below. Our team of scientists (see back) has expertise and experience in each of the areas of the platform and welcomes the opportunity to consult with researchers at any point in the development of their projects.

Product Quality and Value • Strict Confidentiality • Proactive Project Management • Friendly Service

The Complex Custom Peptide Technology Platform

Multi-disulfide Peptides

Lys-22

Shk

Arg-1

Linker

FITC

Biotin

TMR

Peptides International was established in 1983 and is located in Louisville, Kentucky. The PI laboratories, updated and expanded in 2007 and again in 2015/2016, consist of well-equipped R&D labs, a 100-liter reactor system, in-process control instrumentation, purification systems and high-capacity freeze dryers. Incoming raw materials, as well as finished goods, are analyzed using state-of-the-art methods including HPLC, MS, AAA, UV and FTIR spectroscopy, polarimetry, and traditional organic analytical techniques. With these capabilities and the experience of its staff, Peptides International is able to offer effiecient and cost effective solutions to serve the challenging needs of modern peptide-based drug discovery and development.

Interested in mini-proteins with multiple disulfide bonds? PI re-searchers have extensive experience folding these highly com-plicated molecules to their biologically active forms. Examples include native molecules and analogs of conotoxins, defensins, hepcidins, chemokines, ion channel blockers (ShK, HwTX4, charybdotoxin, iberiotoxin, margatoxin, ProTx-II, mu-conotoxins, etc). They can also customize with labels such as TAMRA, FITC or Biotin using a mini-PEGTM spacer (example at right).

• Hydroxyethylene Isostere

• Reduced Peptide Bonds

• Aldehydes

• Chloromethylketones

• Depsipeptides

• Phosphonates

• Glycosylation

• Phosphorylation

• Fatty Acylation

• Pegylation

• Cyclization (head-to-tail, actam, thioether)

• Disulfide Bonds

• Stapling

• Novel Amino Acid Derivatives

• Stereo Selective Synthesis

• Special Protecting Groups

• Natural Product Synthesis

• Chromogenic / Fluorogenic Substrates

Peptide-protein conjugates have a variety of applications, including the stimulation of antibodies for immunization KLH (Keyhole Limpet Hemo-cyanin) (see example at right) or BSA (Bovine Serum Albumin). These conjugates can be customized as needed, including combinations with proteins such as Ovalbumin or Ferritin. Selective heterobifunctional crosslinkers allow for selective attachment of a Cys-containing peptide to the protein of your choice. Using KLH-MCC or Maleimide-Activated BSA will help streamline this reaction, and the products (KLH-6000-PI and BSA-6001-PI, respectively) are conveniently kept in stock at all times.

Peptide-Protein ConjugatesNH

O

N

O

O

NH

O

N

O

O

S

SH Peptide

Peptide

KLH

KLH

MALEIMIDE-ACTIVATED CARRIER PROTEIN

CARRIER-PEPTIDE CONJUGATE

SULFHYDRYL-CONTAINING HAPTEN (PEPTIDE WITH

TERMINAL CYSTEINE)

Fluorescence resonance energy transfer (FRET) occurs when excitation energy is transferred from an excited fluorescent donor to a quenching acceptor in a distance-dependent manner. Cleavage of a scissile peptide bond within a fluorescence quenched substrate leads to separation of the intramolecular donor-acceptor pair, thus allowing an increase in fluorescence. The fluorescence increase is proportional to the amount of peptide hydrolyzed. If you need to design a substrate for a newly discovered enzyme or application, contact our specialists to begin your Substrates by DesignTM project today.

Peptide Derivatives

Post Translational Modification

• Classical/Solution

• Solid-Phase

• Native Chemical Ligation

• Dendrimeric RGD Platforms

Peptidomimetics

Multi-step Organic SynthesisPeptide Synthesis

hν

QUENCHER

DABCYL

ENZYME

EDANS

DONOR

GENERATION OF FLUORESCENT SIGNAL

EFFICIENT QUENCHING OF DONOR FLUORESCENCE

DABCYLEDANS

Contact us for additional examples in these areas

CATALOG PRODUCTS• RGDPlatform• ProTx-II&OtherToxins• BiologicallyActivePeptides• Substrates&Inhibitors• dPEGsandLinkersFulllist@PEPNET.COM

CUSTOM CHEMISTRY SERVICESMadeinUSA• DesignConsultation• ChemistryDevelopment• ProcessScale-up• Pilot-ScaleSynthesis• Solution&SolidPhase• Substrates by DesignTM

TM

Keyliteraturecitationsandpatentsreferring to Peptides Internationalproductsandservices

EXCELLENCE COMPOUNDED• HighPurity,QualityandValue• StrictConfidentiality• FriendlyService• ProactiveProjectManagement

PEPNET.

Your Peptides International Custom Synthesis Team LeadersTwo key advantages, support and expertise, are exemplified by the custom peptide team backing your project. These specialists

have decades of cumulative knowledge, combined with a sense of ownership and dedication to each custom project.

Production

Michael Pennington, Ph.D.President, Chief Executive Officer

Dr.Penningtonhasover25yearsinresearchanddevelopmentmanagement.Hecontinuestoactivelypublishwithmorethan115publications.Hisactiveprojectinvolvementincomplexpeptidesespeciallythosewithmultipledisulfidebondskeepshisfingersonthepulseofthecompany.Hehasservedoneditorialboardsoffourpeptidesciencerelatedjournalsandparticipatesonthescientificadvisoryboardsofseveralbiotechcompanies.

Dr. Satendra Chauhan, Ph.D.Chief Scientific Officer

Dr.Chauhanhasextensiveexpertiseinretrosyntheticanalysis,synthesis,purificationandanalysisofcomplexorganic molecules, peptides andpeptidomimetics as well as totalsynthesis of natural products. Dr.Chauhanhasoverthreedecadesofpeptidelaboratoryexperience.

Andrzej Czerwinski, Ph.D.Senior Chemist

Dr. Czerwinski’s career in peptidechemistryspansover40yearsandhe hasbeena vital PImember forwelloveradecade.Withnumerouspeer-reviewed papers and articlesto his credit,Dr.Czerwinski bringsliterallyalifetimeofexperienceandinsighttoeachproject.

Rosendo Estrada, Ph.D.Production Manager

Dr. Estrada oversees all of theproductioneffortsinthesolid-phaselaboratories. He manages ourproductionchemists fromthesmallscale up through the pilot scaleproduction campaigns which areconductedatPI.Heutilizesourvastarray of state-of-the-art equipmenttoquicklyandefficientlysynthesizeyourproducts.

Vikas Dharwan Ph.D.Purification Manager

Dr.Dhawanhasvastexperienceinboth research peptide purificationas well as commercial drugproduction under cGMP. Vikasbrings this wealth of knowledge tohisteamtoexpertlypurifypeptidesto the most exacting standardswhichyoumayrequest.

Quality Assurance Quality Control Customer Care

Patricia Coxon Ph.D. Director of Quality Management SystemsDr. Coxon assures a strict adherance toquality standards while expediting smoothproject management. She serves as liasonbetween the customer and PI laboratory,securing a stress-free realization for eachcustom project, and that the ISO 9001standardsaremet.

Yingjun Zhang, M.S.Quality Control Manager

YingjunZhangisthefinalauthorityforqualitycontrol,certifyingthatthedatamatchestherequested specifications, for each custompeptideproducedinthePIlaboratory.

Timothy Morgan, M.S.Director of Sales

Withextensiveexperienceineveryaspectof Peptides International’s laboratories,Timothy Morgan is uniquely qualified toshepherd your project throughout theprocesssinaneconomicalandexpeditedmanner,withtheperspectiveofsomeonewhotrulyknows“howit’sdone.”

Peptides International, Inc.11621ElectronDriveLouisville,KY40299USA

phone:+1-502-266-8787tollfreephone:+1-800-777-4779(USandCanada)

fax:+1-502-267-1329email:peptides@pepnet.com